Academic literature on the topic 'Environmental aspects of Microbial biotechnology'

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Journal articles on the topic "Environmental aspects of Microbial biotechnology"

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Leonhäuser, J., M. Röhricht, I. Wagner-Döbler, and W. D. Deckwer. "Reaction Engineering Aspects of Microbial Mercury Removal." Engineering in Life Sciences 6, no. 2 (April 2006): 139–48. http://dx.doi.org/10.1002/elsc.200620904.

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Ermakov, V. V., T. V. Guseva, Yu V. Kovalsky, and V. I. Panfilov. "Applied aspects of the geochemical ecology of microorganisms used for solving ecobiotechnological tasks." Biotekhnologiya 36, no. 6 (2020): 107–14. http://dx.doi.org/10.21519/0234-2758-2020-36-6-107-114.

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The article presents a new view on opportunities on using microorganisms for achieving a wide range of biotechnological and environmental objectives. Examples of recent innovations in applying bio-products based on living cells of microorganisms for remediating oil polluted soils and enhancing soil fertility are given. The significance of microbiological solutions with regards of achieving objectives of the National Project "Environment/Ecology" is underlined. Perspectives and effectiveness of technologies applying microbial biomass enriched in microelements for correcting microelementoses and preventing biogeochemical endemic diseases is demonstrated. biogeochemistry, biotechnology, geochemical ecology, microelementosis, effectiveness of application, microbial bio-products.
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Mutere, Dr Olga. "Editorial: Managing the Microbial Activity in Food and Environmental Biotechnologies." Open Biotechnology Journal 9, no. 1 (June 26, 2015): 30. http://dx.doi.org/10.2174/1874070701509010030.

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The present issue embraces a broad spectrum of studies focused on applied or potentially applied aspects of microbiology and biotechnology. The papers reflect current scientific and technological investigations, which were performed in Latvia, Lithuania, Estonia, Sweden. Most of them are interdisciplinary, i.e., the expertises in microbiology, biochemistry, chemistry, veterinary, physics, engineering and other fields were applied. Probiotic lactic acid bacteria were tested for their antagonistic activity against mastitis causing bacteria. Three papers are focused on optimization of fermentation processes, using maize silage and acid whey in anaerobic processes, barley and pea fibers as well as lupine seeds for obtaining biologically active compounds. Wastewater treatment processes are represented by studies on the use of pink bark as a sorbent of nitroaromatic compounds; as well as biodegradation of phenols in pharmaceutical wastewaters and combination of nitrification and phosphorous accumulation processes. Newly developed inorganic carriers for solid state and submerged fermentations were tested, e.g., ceramic and alkali-activated beads. The review paper covers basic processes of phytoremediation with special emphasis on rhizoremediation and plant-microbe interactions in a plant–assisted biodegradation in soils and treatment wetlands. Methodological aspects were discussed, in particular, the use of Fourier transform infrared spectroscopy in monitoring the sorption and degradation products in wastewaters. A new yeast based test system for rapid evaluation of the effects of various compounds on eukaryotic cells was suggested. Hopefully, you will find the papers included in this issue, interesting and useful.
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Morasch, Barbara, Hans H. Richnow, Bernhard Schink, and Rainer U. Meckenstock. "Stable Hydrogen and Carbon Isotope Fractionation during Microbial Toluene Degradation: Mechanistic and Environmental Aspects." Applied and Environmental Microbiology 67, no. 10 (October 1, 2001): 4842–49. http://dx.doi.org/10.1128/aem.67.10.4842-4849.2001.

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ABSTRACT Primary features of hydrogen and carbon isotope fractionation during toluene degradation were studied to evaluate if analysis of isotope signatures can be used as a tool to monitor biodegradation in contaminated aquifers. D/H hydrogen isotope fractionation during microbial degradation of toluene was measured by gas chromatography. Per-deuterated toluene-d 8 and nonlabeled toluene were supplied in equal amounts as growth substrates, and kinetic isotope fractionation was calculated from the shift of the molar ratios of toluene-d 8 and nondeuterated toluene. The D/H isotope fractionation varied slightly for sulfate-reducing strain TRM1 (slope of curve [b] = −1.219), Desulfobacterium cetonicum(b = −1.196), Thauera aromatica(b = −0.816), and Geobacter metallireducens (b = −1.004) and was greater for the aerobic bacterium Pseudomonas putidamt-2 (b = −2.667). The D/H isotope fractionation was 3 orders of magnitude greater than the13C/12C carbon isotope fractionation reported previously. Hydrogen isotope fractionation with nonlabeled toluene was 1.7 and 6 times less than isotope fractionation with per-deuterated toluene-d 8 and nonlabeled toluene for sulfate-reducing strain TRM1 (b = −0.728) andD. cetonicum (b = −0.198), respectively. Carbon and hydrogen isotope fractionation during toluene degradation by D. cetonicum remained constant over a growth temperature range of 15 to 37°C but varied slightly during degradation by P. putida mt-2, which showed maximum hydrogen isotope fractionation at 20°C (b = −4.086) and minimum fractionation at 35°C (b = −2.138). D/H isotope fractionation was observed only if the deuterium label was located at the methyl group of the toluene molecule which is the site of the initial enzymatic attack on the substrate by the bacterial strains investigated in this study. Use of ring-labeled toluene-d 5 in combination with nondeuterated toluene did not lead to significant D/H isotope fractionation. The activity of the first enzyme in the anaerobic toluene degradation pathway, benzylsuccinate synthase, was measured in cell extracts of D. cetonicum with an initial activity of 3.63 mU (mg of protein)−1. The D/H isotope fractionation (b = −1.580) was 30% greater than that in growth experiments with D. cetonicum. Mass spectroscopic analysis of the product benzylsuccinate showed that H atoms abstracted from the toluene molecules by the enzyme were retained in the same molecules after the product was released. Our findings revealed that the use of deuterium-labeled toluene was appropriate for studying basic features of D/H isotope fractionation. Similar D/H fractionation factors for toluene degradation by anaerobic bacteria, the lack of significant temperature dependence, and the strong fractionation suggest that analysis of D/H fractionation can be used as a sensitive tool to assess degradation activities. Identification of the first enzyme reaction in the pathway as the major fractionating step provides a basis for linking observed isotope fractionation to biochemical reactions.
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Cui, Yang, Bin Lai, and Xinhua Tang. "Microbial Fuel Cell-Based Biosensors." Biosensors 9, no. 3 (July 23, 2019): 92. http://dx.doi.org/10.3390/bios9030092.

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The microbial fuel cell (MFC) is a promising environmental biotechnology that has been proposed mainly for power production and wastewater treatment. Though small power output constrains its application for directly operating most electrical devices, great progress in its chemical, electrochemical, and microbiological aspects has expanded the applications of MFCs into other areas such as the generation of chemicals (e.g., formate or methane), bioremediation of contaminated soils, water desalination, and biosensors. In recent decades, MFC-based biosensors have drawn increasing attention because of their simplicity and sustainability, with applications ranging from the monitoring of water quality (e.g., biochemical oxygen demand (BOD), toxicants) to the detection of air quality (e.g., carbon monoxide, formaldehyde). In this review, we summarize the status quo of MFC-based biosensors, putting emphasis on BOD and toxicity detection. Furthermore, this review covers other applications of MFC-based biosensors, such as DO and microbial activity. Further, challenges and prospects of MFC-based biosensors are briefly discussed.
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Rao, Mala B., Aparna M. Tanksale, Mohini S. Ghatge, and Vasanti V. Deshpande. "Molecular and Biotechnological Aspects of Microbial Proteases." Microbiology and Molecular Biology Reviews 62, no. 3 (September 1, 1998): 597–635. http://dx.doi.org/10.1128/mmbr.62.3.597-635.1998.

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SUMMARY Proteases represent the class of enzymes which occupy a pivotal position with respect to their physiological roles as well as their commercial applications. They perform both degradative and synthetic functions. Since they are physiologically necessary for living organisms, proteases occur ubiquitously in a wide diversity of sources such as plants, animals, and microorganisms. Microbes are an attractive source of proteases owing to the limited space required for their cultivation and their ready susceptibility to genetic manipulation. Proteases are divided into exo- and endopeptidases based on their action at or away from the termini, respectively. They are also classified as serine proteases, aspartic proteases, cysteine proteases, and metalloproteases depending on the nature of the functional group at the active site. Proteases play a critical role in many physiological and pathophysiological processes. Based on their classification, four different types of catalytic mechanisms are operative. Proteases find extensive applications in the food and dairy industries. Alkaline proteases hold a great potential for application in the detergent and leather industries due to the increasing trend to develop environmentally friendly technologies. There is a renaissance of interest in using proteolytic enzymes as targets for developing therapeutic agents. Protease genes from several bacteria, fungi, and viruses have been cloned and sequenced with the prime aims of (i) overproduction of the enzyme by gene amplification, (ii) delineation of the role of the enzyme in pathogenecity, and (iii) alteration in enzyme properties to suit its commercial application. Protein engineering techniques have been exploited to obtain proteases which show unique specificity and/or enhanced stability at high temperature or pH or in the presence of detergents and to understand the structure-function relationships of the enzyme. Protein sequences of acidic, alkaline, and neutral proteases from diverse origins have been analyzed with the aim of studying their evolutionary relationships. Despite the extensive research on several aspects of proteases, there is a paucity of knowledge about the roles that govern the diverse specificity of these enzymes. Deciphering these secrets would enable us to exploit proteases for their applications in biotechnology.
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Paredes, D., P. Kuschk, T. S. A. Mbwette, F. Stange, R. A. Müller, and H. Köser. "New Aspects of Microbial Nitrogen Transformations in the Context of Wastewater Treatment – A Review." Engineering in Life Sciences 7, no. 1 (February 2007): 13–25. http://dx.doi.org/10.1002/elsc.200620170.

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Adriaenssens, Evelien M., and Don A. Cowan. "Using Signature Genes as Tools To Assess Environmental Viral Ecology and Diversity." Applied and Environmental Microbiology 80, no. 15 (May 16, 2014): 4470–80. http://dx.doi.org/10.1128/aem.00878-14.

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ABSTRACTViruses (including bacteriophages) are the most abundant biological entities on the planet. As such, they are thought to have a major impact on all aspects of microbial community structure and function. Despite this critical role in ecosystem processes, the study of virus/phage diversity has lagged far behind parallel studies of the bacterial and eukaryotic kingdoms, largely due to the absence of any universal phylogenetic marker. Here we review the development and use of signature genes to investigate viral diversity, as a viable strategy for data sets of specific virus groups. Genes that have been used include those encoding structural proteins, such as portal protein, major capsid protein, and tail sheath protein, auxiliary metabolism genes, such aspsbA,psbB, andphoH, and several polymerase genes. These marker genes have been used in combination with PCR-based fingerprinting and/or sequencing strategies to investigate spatial, temporal, and seasonal variations and diversity in a wide range of habitats.
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Kieliszek, Marek, Kamil Piwowarek, Anna M. Kot, and Katarzyna Pobiega. "The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry." Open Life Sciences 15, no. 1 (October 22, 2020): 787–96. http://dx.doi.org/10.1515/biol-2020-0099.

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AbstractCellular biomass of microorganisms can be effectively used in the treatment of waste from various branches of the agro-food industry. Urbanization processes and economic development, which have been intensifying in recent decades, lead to the degradation of the natural environment. In the first half of the 20th century, problems related to waste management were not as serious and challenging as they are today. The present situation forces the use of modern technologies and the creation of innovative solutions for environmental protection. Waste of industrial origin are difficult to recycle and require a high financial outlay, while the organic waste of animal and plant origins, such as potato wastewater, whey, lignin, and cellulose, is dominant. In this article, we describe the possibilities of using microorganisms for the utilization of various waste products. A solution to reduce the costs of waste disposal is the use of yeast biomass. Management of waste products using yeast biomass has made it possible to generate new metabolites, such as β-glucans, vitamins, carotenoids, and enzymes, which have a wide range of industrial applications. Exploration and discovery of new areas of applications of yeast, fungal, and bacteria cells can lead to an increase in their effective use in many fields of biotechnology.
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Kumar, Shashank, and Abhay K. Pandey. "Chemistry and Biological Activities of Flavonoids: An Overview." Scientific World Journal 2013 (2013): 1–16. http://dx.doi.org/10.1155/2013/162750.

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There has been increasing interest in the research on flavonoids from plant sources because of their versatile health benefits reported in various epidemiological studies. Since flavonoids are directly associated with human dietary ingredients and health, there is need to evaluate structure and function relationship. The bioavailability, metabolism, and biological activity of flavonoids depend upon the configuration, total number of hydroxyl groups, and substitution of functional groups about their nuclear structure. Fruits and vegetables are the main dietary sources of flavonoids for humans, along with tea and wine. Most recent researches have focused on the health aspects of flavonoids for humans. Many flavonoids are shown to have antioxidative activity, free radical scavenging capacity, coronary heart disease prevention, hepatoprotective, anti-inflammatory, and anticancer activities, while some flavonoids exhibit potential antiviral activities. In plant systems, flavonoids help in combating oxidative stress and act as growth regulators. For pharmaceutical purposes cost-effective bulk production of different types of flavonoids has been made possible with the help of microbial biotechnology. This review highlights the structural features of flavonoids, their beneficial roles in human health, and significance in plants as well as their microbial production.
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Dissertations / Theses on the topic "Environmental aspects of Microbial biotechnology"

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Yeh, Daniel H. "Influence of nonionic surfactants on the bioavailability and microbial reductive dechlorination of hexachlorobenzene." Diss., Georgia Institute of Technology, 2000. http://hdl.handle.net/1853/20200.

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Booker, Randall Sulter Jr. "Microbial reductive dechlorination of hexachloro-1,3-butadiene." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/20921.

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Jackson, Vanessa A. (Vanessa Angela). "Microbial response to oxidising biocides." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53499.

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Thesis (MSc)--University of Stellenbosch, 2003.
ENGLISH ABSTRACT: Biofouling of water systems is a problem extensively experienced in industry. Although this subject is the focus of many studies, the ability of microorganisms to survive exposure to biocides is still poorly understood. This study aimed to assess the biocidal effect of ozone on planktonic cells and biofilm communities, to evaluate different ozone generation techniques, and to follow population shifts within the biofilm community. Specific objectives included determining the effect of different ozone concentrations, the effect of different exposure times, and an assessment of microbial responses after exposure to sub-lethal ozone concentrations. Typically, 300 ml of an ovemight bacterial culture was exposed to ozone that was generated by anodic oxidation (0.3% wt or 18- 20% wt, respectively) or silent electric discharge (3.5% wt 03). The ozone was purged into the culture for 5-, 7-, 10- and 15 min., respectively. Enumeration of cells following ~10 min. exposure to 18-20% wt ozone showed a significant reduction in viable cell numbers. In contrast, when exposed to the two lower 03 concentrations, there was little change in the viable cell numbers even after prolonged exposure (30- and 60 min.). To evaluate biofilms, ozone was bubbled into the irrigation that was pumped through replicate flow cell channels. Response to ozone exposure was evaluated after staining the biofilms with the Baclight Viability probe, observation with fluorescence microscopy, and image analysis. The higher ozone concentration (18-20% wt 03) more effectively disrupted the biofilm structure of denser biofilms than the lower concentration, especially after 90 min. exposure. When compared to the controls, the 90 min. exposure resulted in a notable reduction in viable cells from 69% to 38% and a corresponding increase in nonviable cells from 29% to 62%. The lower concentration ozone (3.5% wt 03) was effective against the less dense, thinner biofilms evaluated, but not effective against the thicker biofilm. An analysis of the differences between continuous culture biofilms and batch culture biofilms showed that the biofilms in the batch system were less rigid. To evaluate microbial response to biocides, techniques such as Biolog whole-community metabolic profiles and terminal restriction fragment length polymorphisms (T-RFLP) were used. Biolog analysis of planktonic cells revealed changes following exposure to sub-lethal biocide concentrations, however carbon utilisation profiles resembled that of the controls after 24-48 hours. For biofilm communities, no carbon utilization differences could be detected under these conditions. There was, however differences in T-RFLP patterns between treated and untreated biofilm communities.
AFRIKAANSE OPSOMMING: Biobevuiling van watersisteme is 'n probleem wat algemeen in industriëe ervaar word. Alhoewel hierdie onderwerp die fokus van vele studies is, word die vermoëns van mikroorganismes om blootstelling aan biosiede te weerstaan steeds swak verstaan. Die doel van hierdie studie was om die biosidiese effek van osoon op planktoniese selle en biofilm gemeenskappe waar te neem, om die verskillende osoon generasie tegnieke te evalueer, asook om verskuiwings in die samestelling van die biofilm gemeenskap waar te neem. Spesifieke doelwitte sluit in die bepaling van die effek van verskillende osoon konsentrasies, die blootstellingtye, en 'n waarneming van mikrobiese reaksies na blootstelling aan sub-dodings osoon konsentrasies. Drie honderd ml van 'n oornag bakteriese kultuur was aan osoon, wat deur anodiese oksidasie (0.3% wt of 18% - 20% wt) of geluidlose elektriese ontlading (3.5% wt), gegenereer is, blootgestel. Tye van blootstelling was 5-, 7-, 10-, of 15 min., onderskeidelik. Bepaling van selgetalle na :2:10 min. blootstelling aan 18 - 20% wt osoon, het 'n betekenisvolle verlaging in die getal lewensvatbare mikrobeselle getoon. In teenstelling hiermee, het blootstelling aan twee laer osoon konsentrasies min verskil in die lewensvatbare selgetalle, selfs na verlengde blootstellingstye (30- en 60 min.), getoon. Om biofilms te evalueer is osoon in die medium geborrel wat deur replikaat vloeisel kanale gepomp is. Na osoon blootstelling, was die vloeisel onderwerp aan beeld analise deur gebruik te maak van die Baclight lewensvatbare peiler en fluoressensie mikroskopie. Die hoër osoon konsentrasie (18 - 20% wt 03) het die struktuur van dikker biofilms meer effektiefuiteengeskeur as die laer konsentrasie, veral na 90 min. blootstelling. In vergelyking met die onderskeie kontroles, het die getalle van lewensvatbare selle na 90 min. blootstelling drasties verlaag vanaf 69% tot 38% en 'n ooreenstemmende toename in die nie-lewensvatbare selgetalle vanaf 29% tot 62%. Die laer osoon konsentrasie (3.5% wt 03) was meer effektief teenoor die minder digte en dunner biofilms wat ge-evalueer was, maar nie so effektief teenoor die dikker biofilms nie. 'n Analise van die verskille tussen kontinue-kultuur biofilms en lotkultuur biofilms het getoon dat die lot-kultuur biofilms minder rigied is. Vir die evaluering van mikrobiese reaksies na biosied blootstelling, is tegnieke soos Biolog gemeenskap metaboliese profiele en eind-restriksie-fragment-lengte polimorfisme (TRFLP) gebruik. Biolog analise van planktoniese selle het verskille getoon na blootstelling aan sub-dodelike biosied konsentrasies. Koolstof benutting het wel na 24 - 48 ure met dit van die kontrole ooreengestem. Vir biofilm gemeenskappe was daar geen noemenswaardige verskille in koolstof benutting nie. Daar was wel verskille in T-RFLP patrone tussen die onbehandelde en biosied-behandelde biofilm gemeenskappe.
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Prytula, Mark Taras. "Bioavailability and microbial dehalogenation of chlorinated benzenes sorbed to estuarine sediments." Diss., Georgia Institute of Technology, 1998. http://hdl.handle.net/1853/32835.

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Scanferlato, Vjera Sostarec. "Environment risk assessment for toxic chemicals and genetically-engineered microorganisms : a microcosm approach /." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-07282008-135357/.

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Krige, Adolf. "Microbial Fuel cells, applications and biofilm characterization." Licentiate thesis, Luleå tekniska universitet, Kemiteknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-73938.

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Since the 1900’s it has been known that microorganisms are capable of generating electrical power through extracellular electron transfer by converting the energy found organic compounds (Potter, 1911). Microbial fuel cells (MFCs) has garnered more attention recently, and have shown promise in several applications, including wastewater treatment (Yakar et al., 2018), bioremediation (Rosenbaum & Franks, 2014), biosensors (ElMekawy et al., 2018) desalination (Zhang et al., 2018) and as an alternative renewable energy source in remote areas (Castro et al., 2014). In MFCs catalytic reactions of microorganisms oxidize an electron donor through extracellular electron transfer to the anode, under anaerobic conditions, with the cathode exposed to an electron acceptor, facilitating an electrical current (Zhuwei, Haoran & Tingyue, 2007; Lovley, 2006). For energy production in remote areas a low cost and easily accessible feed stock is required for the MFCs. Sweet sorghum is a drought tolerant feedstock with high biomass and sugar yields, good water-use efficiency, established production systems and the potential for genetic improvements. Because of these advantages sweet sorghum stalks were proposed as an attractive feedstock (Rooney et al., 2010; Matsakas & Christakopoulos, 2013). Dried sweet sorghum stalks were, therefore, tested as a raw material for power generation in a MFC, with anaerobic sludge from a biogas plant as inoculum (Sjöblom et al., 2017a). Using sorghum stalks the maximum voltage obtained was 546±10 mV, the maximum power and current density of 131±8 mW/m2 and 543±29 mA/m2 respectively and the coulombic efficiency was 2.2±0.5%. The Ohmic resistances were dominant, at an internal resistance of 182±17 Ω, calculated from polarization data. Furthermore, hydrolysis of the dried sorghum stalks did not improve the performance of the MFC but slightly increased the total energy per gram of substrate. During the MFC operation, the sugars were quickly fermented to formate, acetate, butyrate, lactate and propionate with acetate and butyrate being the key acids during electricity generation. Efficient electron transfer between the microorganisms and the electrodes is an essential aspect of bio-electrochemical systems such as microbial fuel cells. In order to design more efficient reactors and to modify microorganisms, for enhanced electricity production, understanding the mechanisms and dynamics of the electron transport chain is important. It has been found that outer membrane C-type cytochromes (OMCs) (including omcS and omcZ discussed in this study) play a key role in the electron transport chain of Geobacter sulfurreducens, a well-known, biofilm forming, electro-active microorganism  (Millo et al., 2011; Lovley, 2008). It was found that Raman microscopy is capable of providing biochemical information, i.e., the redox state of c-type cytochromes (cyt-C) without damaging the microbial biofilm, allowing for in-situ observation. Raman microscopy was used to observe the oxidation state of OMCs in a suspended culture, as well as in a biofilm of an MFC. First, the oxidation state of the OMCs of suspended cultures from three G. sulfurreducens strains (PCA, KN400 and ΔpilA) was analyzed. It was found that the oxidation state can also be used as an indicator of the metabolic state of the cells, and it was confirmed that PilA, a structural pilin protein essential for long range electron transfer, is not required for external electron transfer. Furthermore, we designed a continuous, anaerobic MFC enabling in-situ Raman measurements of G. sulfurreducens biofilms during electricity generation, while poised using a potentiostat, in order to monitor and characterize the biofilm. Two strains were used, a wild strain, PCA, and a mutant, ΔOmcS. The cytochrome redox state, observed through the Raman spectra, could be altered by applying different poise voltages to the electrodes. This change was indirectly proportional to the modulation of current transferred from the cytochromes to the electrode. This change in Raman peak area was reproducible and reversible, indicating that the system could be used, in-situ, to analyze the oxidation state of proteins responsible for the electron transfer process and the kinetics thereof.
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Penfield, Tyler. "Microbial communities in an anaerobic membrane bioreactor (AnMBR) treating domestic wastewater at ambient temperatures in a temperate climate." Thesis, Kansas State University, 2017. http://hdl.handle.net/2097/38197.

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Master of Science
Department of Civil Engineering
Prathap Parameswaran
The ever-increasing demand for water, food, and energy and the simultaneous diminishment of our planets’ ecosystems wrought by humans have prompted a more sustainable approach to engineering the built environment. Wastewater treatment systems stand at the interface that connects the built and natural environment where potential solutions for resource and environmental issues exist. Wastewater treatment technologies can address issues involving water, food, energy, and environmental regulation when resources are properly captured from the wastewater while it’s being treated. This way of thought allows wastewater to be perceived as a source of valuable products rather than an obligate waste stream. For this reason, anaerobic wastewater treatment is progressively being considered because of its ability to improve energy and resource recovery, while reducing costs and environmental impacts associated with conventional domestic wastewater treatment. More specifically, anaerobic membrane bioreactors (AnMBRs) hold promise to effectively treat wastewater at low temperatures with low energy and nutrient requirements, low sludge production, while having the benefit of generating methane-rich biogas suitable as an energy source and the potential to capture nutrients used to fertilize cropland. But, at low temperatures the microbial communities that control anaerobic digestion (AD) face biochemical obstacles. Elucidating the microbial community dynamics within AnMBRs with respect to seasonal temperatures will give insight on how to efficiently operate AnMBRs with the goal of energy-neutral wastewater treatment. DNA based tools such as advanced high-throughput sequencing was coupled with AnMBR process data to explicate the mechanism of methane production in the suspended biomass of an AnMBR from a mesophilic startup leading into psychrophilic conditions, and then returning to mesophilic temperatures.
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Godow, Bratt Tora, Mathilda Stigenberg, Andreas Elenborg, Sarah Ågren, and Andreas Medhage. "To monitor the microbial biodiversity in soil within Uppsala." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-444210.

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This is an exploration of the potential for a citizen science project, with the goal to get the general public involved in microbial soil biodiversity around Uppsala, Sweden. Biodiversity serves an important role in how an ecosystem performs and functions. A large part of Earth's biodiversity exists below ground in soil, where microorganisms interact with plants. It would be beneficial to analyse the abundance and spread of some microorganisms in order to gain a better understanding of soil biodiversity. We suggest that one species family to study could be Phytophthora. Phytophthora is a genus of oomycetes that often are pathogenic, causing disease in various trees and other plants. It is unknown exactly how widespread the genus is today, making it extra interesting for the proposed study. For the general public to be able to do this a device needs to be developed that is easy to use and preferably could be used directly in the field. An isothermal amplification method is suitable for identifying the microorganism under these conditions. Many isothermal amplification methods are expensive, perhaps too expensive for a citizen science study, but have great potential for easy field testing. We propose a device utilizing RPA and lateral flow strips. RPA - Recombinase Polymerase Amplification is a method for amplification that might be suitable since it is simple, sensitive, and has a short run time. It is however expensive, which is an issue, but isothermal amplifications are expensive across the board. Lateral flow strips can be used to visualize the results. They utilize antibodies to detect the previously amplified amplicons, and give a positive or negative test answer that would be understandable to even untrained study participants. One of the biggest obstacles identified in this project concerns amplifying DNA from a soil sample, because an extraction step is necessary. The methods we have identified for extraction are not performable in the field, since they require centrifugation. In the proposition for a device a possible work-around for this is proposed, but since it has yet to be tested it is not yet known whether it will work or not.
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Mutambanengwe, Cecil Clifford Zvandada. "The biotechnology of hard coal utilization as a bioprocess substrate." Thesis, Rhodes University, 2010. http://hdl.handle.net/10962/d1003993.

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The development of coal biotechnology, using hard coal as a substrate, has been impeded by its low reactivity in biological processes. As a result, the more successful application studies have focused on lignitic soft coals. However, new studies have reported using biologically or geologically oxidized hard coal as a functional substrate option for bioprocess applications on a large scale. This study undertook a preliminary investigation into the feasibility of environmental applications of coal biotechnology using oxidized hard coal substrates in both anaerobic and aerobic processes with carbon dioxide, sulfate and oxygen as terminal electron acceptors. A preliminary characterization of the oxidized hard coal substrates was undertaken to determine and predict their viability and behavior as electron donors and carbon sources for environmental bioprocess applications of direct interest to the coal mining industry. Both biologically and geologically oxidized coal substrates showed loss of up to 17% and 52% carbon respectively and incorporation of oxygen ranging from 0.9 – 24%. The latter substrate showed greater loss of carbon and increased oxygenation. The biologically and geologically oxidized hard coal substrates were shown to partition readily into 23% and 32% organic humic acid, a 0.1% fulvic acid fraction and 65% and 59% inorganic and humin fractions respectively. These organic components were shown to be potentially available for biological consumption. In the unmodified hard coal substrate, partitioning was not observed and it did not perform as a functional substrate for any of the bioprocesses investigated. Where carbon dioxide was used as a terminal electron acceptor, methane production ranging from 9 – 26 mg CH4.g substrate-1 was demonstrated from both oxidized coal substrates. Geologically oxidized coal produced 30% more methane than biologically oxidized coal. Methane yields from the geologically oxidized coal in the presence and absence of a co-substrate were 5 – 13-fold higher than previous studies that used hard coal for methanogenesis. Based on these results, and that the development and optimization of the biological oxidation process is currently ongoing, further applications investigated in this study were undertaken using geologically oxidized coal. It was shown using pyrolysis gas chromatography mass spectrometry that the methanogenic system was dependent on the presence of an effective co-substrate supporting the breakdown of the complex organic structures within the oxidized hard coal substrate. Also that the accumulation of aromatic intermediate breakdown compounds predominantly including toluene, furfural, styrene and 2-methoxy vinyl phenol appeared to become inhibitory to both methanogenic and sulfidogenic reactions. This was shown to be a more likely cause of reactor failure rather than substrate exhaustion over time. Evidence of a reductive degradation pathway of the complex organic structures within the oxidized hard coal substrates was shown through the production, accumulation and utilization of volatile fatty acids including acetic, formic, propionic, butyric and valeric acids. Comparative analysis of the volatile fatty acids produced in this system showed that geologically oxidized coal produced 20% more of the volatile fatty acids profiled and double the total concentration compared to the biologically oxidized coal. The use of geologically oxidized hard coal as a functional substrate for biological sulfate reduction was demonstrated in the neutralization of a simulated acid mine drainage wastewater in both batch and continuous process operations. Results showed an increase in pH from pH 4.0 to ~ pH 8.0 with sulfide production rates of ~ 86 mgL-1.day-1 in the batch reactions, while the pH increased to pH 9.0 and sulfide production rates of up to 450 mgL-1.day-1 were measured in the continuous process studies using sand and coal up-flow packed bed reactors. Again, the requirement for an effective co-substrate was demonstrated with lactate shown to function as a true co-substrate in this system. However, a low cost alternative to lactate would need to emerge if the process was to function in large-scale commercial environmental treatment applications. In this regard, the aerobic growth and production of Neosartorya fischeri biomass (0.64 g.biomass.g SOC-1) was demonstrated using oxidized hard coal and glutamate as a co-substrate. Both can be produced from wastes generated on coal mines, with the fungal biomass generated in potentially large volumes. Preliminary demonstration of the use of the fungal biomass as a carbon and electron donor source for biological sulfate reduction was shown and thus that this could serve as an effective substrate for anaerobic environmental treatment processes. Based on these findings, an Integrated Coal Bioprocess model was proposed using oxidized hard coal as a substrate for environmental remediation applications on coal mines. In this approach, potential applications included methane recovery from waste coal, use of waste coal in the treatment of acid mine drainage waste waters and the recovery and use of humic acids in the rehabilitation of open cast mining soils. This study provided a first report demonstrating the use of biologically and geologically oxidized hard coals as bioprocess substrates in environmental bioremediation applications. It also provided an indication that follow-up bioengineering studies to investigate scaled-up applications of these findings would be warranted.
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10

Cluff, Maryam Ansari. "Microbial Aspects of Shale Flowback Fluids and Response to Hydraulic Fracturing Fluids." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366292190.

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Books on the topic "Environmental aspects of Microbial biotechnology"

1

Biodiversity and environmental biotechnology. Jodhpur: Scientific Publishers (India), 2007.

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Reducing Risks from Environmental Chemicals through Biotechnology (Conference) (1987 Seattle). Environmental biotechnology: Reducing risks from environmental chemicals through biotechnology. New York: Plenum, 1988.

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J, Gauthier Michel, ed. Gene transfers and environment: Proceedings of the Third European Meeting on Bacterial Genetics and Ecology (BAGECO-3), 20-22 November 1991, Villefranche-sur-Mer, France. Berlin: Springer-Verlag, 1992.

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Anton, Blažej, and Prívarová V, eds. Environmental biotechnology: Proceedings of the International Symposium on Biotechnology, Bratislava, Czecho-Slovakia, June 27-29, 1990. Amsterdam: Elsevier, 1991.

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International Symposium on Biotechnology. (1991 Oostende, Belgium). Environmental biotechnology: International symposium, 22-25 April 1991, Oostende, Belgium. Oostende, Belgium: Royal Flemish Society of Engineers, 1991.

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Pelmont, Jean. Biodégradations et métabolismes: Les bactéries pour les technologies de l'environment. Les Ulis: EDP Science, 2005.

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Sukla, Lala Behari, Nilotpala Pradhan, Sandeep Panda, and Barada Kanta Mishra, eds. Environmental Microbial Biotechnology. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-19018-1.

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Sytze, Keuning, and Janssen Dick B, eds. Handbook on biodegradation and biological treatment of hazardous organic compounds. Dordrecht: Kluwer Academic Publishers, 1998.

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Microbial biotechnology: Energy and environment. Wallingford, Oxfordshire: CAB International, 2012.

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Fulekar, M. H. Environmental biotechnology. Enfield, N.H: Science Publishers, 2010.

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Book chapters on the topic "Environmental aspects of Microbial biotechnology"

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Putatunda, Chayanika, Abhishek Walia, Rashmi Sharma, and Preeti Solanki. "Current Trends and Aspects of Microbiological Biogas Production." In Environmental and Microbial Biotechnology, 265–97. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2817-0_12.

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Wattiau, Pierre. "Microbial Aspects in Bioremediation of Soils Polluted by Polyaromatic Hydrocarbons." In Biotechnology for the Environment: Strategy and Fundamentals, 69–89. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-010-0357-5_5.

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Oren, Aharon. "Microbial Systematics." In Environmental Biotechnology, 81–120. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60327-140-0_3.

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Panikov, Nicolai S. "Microbial Ecology." In Environmental Biotechnology, 121–91. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60327-140-0_4.

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Jørgensen, Claus, Jens Aamand, Bjørn K. Jensen, Steffen D. Nielsen, and Carsten Suhr Jacobsen. "Microbial Properties Governing the Microbial Degradation of Polycyclic Aromatic Hydrocarbons." In Environmental Biotechnology, 178–92. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-017-1435-8_17.

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Saunders, Venetia A., and Jon R. Saunders. "Environmental Biotechnology." In Microbial Genetics Applied to Biotechnology, 384–406. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9796-4_9.

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Korzhenevich, V. I., E. V. Volchenko, I. N. Singircev, A. Yu Feodorov, and G. M. Shoob. "Microbial Treatment of Phenolic Wastes." In Environmental Biotechnology, 498–503. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-017-1435-8_43.

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Kynadi, Anna S., and T. V. Suchithra. "Bacterial Degradation of Phenol to Control Environmental Pollution." In Microbial Biotechnology, 245–63. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6847-8_11.

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Sahoo, Sabuj, Sarmistha Sarangi, and Rout George Kerry. "Bioprospecting of Endophytes for Agricultural and Environmental Sustainability." In Microbial Biotechnology, 429–58. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6847-8_19.

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Karlson, U., and W. T. Frankenberger. "Microbial Volatilization of Selenium from Soils." In Environmental Biotechnology, 449. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4899-0824-7_42.

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Conference papers on the topic "Environmental aspects of Microbial biotechnology"

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Sengupta, Debanjan. "Application of Biotechnology in Petroleum Industry - Microbial Enhanced Oil Recovery." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0088.

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"Analysis of Biofilter and Microbial community change under the treatment of Ammonia and Toluene." In International Conference on Biotechnology, Nanotechnology and Environmental Engineering. International Academy of Arts, Science & Technology, 2015. http://dx.doi.org/10.15242/iaast.a0415055.

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Wellman, Dawn M., Shas V. Mattigod, Susan Hubbard, Ann Miracle, Lirong Zhong, Martin Foote, Yuxin Wu, and Danielle Jansik. "Advanced Remedial Methods for Metals and Radionuclides in Vadose Zone Environments." In ASME 2010 13th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2010. http://dx.doi.org/10.1115/icem2010-40235.

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Abstract:
Functionally, the methods for addressing contamination must remove and/or reduce transport or toxicity of contaminants. This problem is particularly challenging in arid environments where the vadose zone can be up to hundreds of feet thick, rendering transitional excavation methods exceedingly costly and ineffective. Delivery of remedial amendments is one of the most challenging and critical aspects for all remedy-based approaches. The conventional approach for delivery is through injection of aqueous remedial solutions. However, heterogeneous vadose zone environments present hydrologic and geochemical challenges that limit the effectiveness. Because the flow of solution infiltration is dominantly controlled by gravity and suction, injected liquid preferentially percolates through highly permeable pathways, by-passing low-permeability zones which frequently contain the majority of the contamination. Moreover, the wetting front can readily mobilize and enhance contaminant transport to underlying aquifers prior to stabilization. Development of innovative, in-situ technologies may be the only way to meet remedial action objectives and long-term stewardship goals. Shear-thinning fluids (i.e., surfactants) can be used to lower the liquid surface tension and create stabile foams, which readily penetrate low permeability zones. Although surfactant foams have been utilized for subsurface mobilization efforts in the oil and gas industry, so far, the concept of using foams as a delivery mechanism for transporting reactive remedial amendments into deep vadose zone environments to stabilize metal and long-lived radionuclide contaminants has not been explored. Foam flow can be directed by pressure gradients, rather than being dominated by gravity; and, foam delivery mechanisms limit the volume of water (< 20% vol.) required for remedy delivery and emplacement, thus mitigating contaminant mobilization. We will present the results of a numerical modeling and integrated laboratory-/intermediate-scale investigation to simulate, develop, demonstrate, and monitor (i.e. advanced geophysical techniques and advanced predictive microbial markers) foam-based delivery of remedial amendments to remediate metals and radionuclides in vadose zone environments.
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Reports on the topic "Environmental aspects of Microbial biotechnology"

1

Zylstra, Gerben, and Jan Roelof van der Meer. Environmental Shortcourse Final report [Joint US-EC Short Course on Environmental Biotechnology: Microbial Catalysts for the Environment]. Office of Scientific and Technical Information (OSTI), March 2013. http://dx.doi.org/10.2172/1067374.

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