Academic literature on the topic 'Environmental marine biotechnology'

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Journal articles on the topic "Environmental marine biotechnology"

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Pomponi, Shirley A., Daniel G. Baden, and Yonathan Zohar. "Marine Biotechnology: Realizing the Potential." Marine Technology Society Journal 41, no. 3 (September 1, 2007): 24–31. http://dx.doi.org/10.4031/002533207787442132.

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Marine biotechnology is an applied science, the goal of which is to develop goods and services from marine organisms and processes. The new wave of marine biotechnology research began in the early 1980s and includes some significant success stories. A new drug to manage pain is commercially available, and a new cancer drug has been recommended for approval, the first from a fish-eating snail and the second from a mangrove tunicate. Enzymes from hydrothermal vent microbes are routinely used in PCR reactions, and marine-derived molecular probes are helping understand the molecular basis of disease processes. Advances in aquaculture biotechnology have resulted in more efficient production of finfish and shellfish for human consumption, and polyunsaturated fatty acids from marine microalgae are used as nutritional supplements for adults and infants. Rapid diagnostic tools have been developed to monitor toxins in the environment and in seafood, and genetic fingerprinting techniques are helping to control illegal trade of threatened marine species. In the future, multidisciplinary programs in oceans and human health should focus not only on microbial pathogens and harmful algal bloom toxins but also on discovery of new chemicals to prevent or treat human diseases. And the development of biological and biochemical sensors to detect pathogens, contaminants, and toxins and to monitor human and environmental health indicators in the marine environment should be a very high priority in the establishment of U.S. coastal ocean observing systems.
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Goddard, Stephen. "Marine biotechnology: Emerging opportunities and future perspectives." Journal of Agricultural and Marine Sciences [JAMS] 20 (January 1, 2015): 7. http://dx.doi.org/10.24200/jams.vol20iss0pp7-7.

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The rapid growth of genetic, cellular and molecular technologies is enabling scientists to explore and develop marine resources for widespread applications in the food, medical, pharmaceutical, environmental and energy industries. Marine biotechnology products and services were estimated at 2.8 billion euros in 2010, with a cumulative annual growth rate of 4-5% (Marine Board-European Science Foundation, 2010) The Sultanate of Oman occupies a strategic geographical position and has a coastline in excess of 3000km, with the Arabian Sea located to the south and the Sea of Oman and Arabian Gulf to the north. These oceans have rich biodiversity and potential resources which we are only beginning to explore. Based on its marine resources, coupled with a rapidly-growing educational and research infrastructure, the Sultanate of Oman is well positioned to take advantage of the commercial opportunities presented by marine biotechnology. In recognition of potential development an international symposium was organized by the Center of Excellence in Marine Biotechnology, Sultan Qaboos University. One hundred and forty eight delegates attended the meeting, representing 15 countries. In planning the symposium three major themes were identified viz. Marine Biofouling and its Prevention, Fisheries and Aquaculture Biotechnology and Marine Biodiversity and Natural Products. The topics were selected on the basis of current and planned research activity in Oman and elsewhere in the GCC region. Three keynote addresses were presented, 23 oral presentations made and a poster exhibition held. A final session reviewed national and regional issues and the delegates agreed formally on a number of future actions. Full details of the symposium and the results and analysis of a post-symposium survey on the future development of marine biotechnology are given in Goddard et al. (2015).The symposium was supported logistically and financially by Sultan Qaboos University, The Research Council, and the
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Tian, Limei, Yue Yin, Wei Bing, and E. Jin. "Antifouling Technology Trends in Marine Environmental Protection." Journal of Bionic Engineering 18, no. 2 (March 2021): 239–63. http://dx.doi.org/10.1007/s42235-021-0017-z.

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AbstractMarine fouling is a worldwide problem, which is harmful to the global marine ecological environment and economic benefits. The traditional antifouling strategy usually uses toxic antifouling agents, which gradually exposes a serious environmental problem. Therefore, green, long-term, broad-spectrum and eco-friendly antifouling technologies have been the main target of engineers and researchers. In recent years, many eco-friendly antifouling technologies with broad application prospects have been developed based on the low toxicity and non-toxicity antifouling agents and materials. In this review, contemporary eco-friendly antifouling technologies and materials are summarized into bionic antifouling and non-bionic antifouling strategies (2000–2020). Non-bionic antifouling technologies mainly include protein resistant polymers, antifoulant releasing coatings, foul release coatings, conductive antifouling coatings and photodynamic antifouling technology. Bionic antifouling technologies mainly include the simulated shark skin, whale skin, dolphin skin, coral tentacles, lotus leaves and other biology structures. Brief future research directions and challenges are also discussed in the end, and we expect that this review would boost the development of marine antifouling technologies.
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Nicoletti, Rosario, and Francesco Vinale. "Bioactive Compounds from Marine-Derived Aspergillus, Penicillium, Talaromyces and Trichoderma Species." Marine Drugs 16, no. 11 (October 26, 2018): 408. http://dx.doi.org/10.3390/md16110408.

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The impact of bioactive compounds from natural sources on human life, particularly in pharmacology and biotechnology, has challenged the scientific community to explore new environmental contexts and the associated microbial diversity. [...]
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de la Calle, Fernando. "Marine Genetic Resources. A Source of New Drugs The Experience of the Biotechnology Sector." International Journal of Marine and Coastal Law 24, no. 2 (2009): 209–20. http://dx.doi.org/10.1163/157180809x421743.

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AbstractThis article provides an overview of the conversion of marine genetic resources into new drugs. Three marine organisms suitable for application in human health and steps in the drug discovery process are described. Specific supply problems resulting from the minute concentration of required compounds for medicine in the natural marine source are examined. Three case studies illustrate different strategies enabling an industrial-scale production: chemical synthesis, biotechnology and fermentation. Future concepts for marine scientific research which could lead to new medical applications are considered. Besides research in unexplored deep sea areas, the “metagenomic approach” particularly might lead to significant new achievements. This DNA analysis of marine organisms facilitates the drug discovery process because it requires significantly less marine material than current approaches. Because this process, starting with the search for bioactive molecules and continuing with the production of drug-like molecules before finally reaching the status of medicine, can take up to 20 years, the development of medicine is a very long and risky venture.
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Schmidt, Eric W. "From chemical structure to environmental biosynthetic pathways: navigating marine invertebrate–bacteria associations." Trends in Biotechnology 23, no. 9 (September 2005): 437–40. http://dx.doi.org/10.1016/j.tibtech.2005.07.002.

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Kalogerakis, Nicolas, Johanne Arff, Ibrahim M. Banat, Ole Jacob Broch, Daniele Daffonchio, Torgeir Edvardsen, Harkaitz Eguiraun, et al. "The role of environmental biotechnology in exploring, exploiting, monitoring, preserving, protecting and decontaminating the marine environment." New Biotechnology 32, no. 1 (January 2015): 157–67. http://dx.doi.org/10.1016/j.nbt.2014.03.007.

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Nielsen, Michael, Lars Hauer Larsen, Mike S. M. Jetten, and Niels Peter Revsbech. "Bacterium-Based NO2− Biosensor for Environmental Applications." Applied and Environmental Microbiology 70, no. 11 (November 2004): 6551–58. http://dx.doi.org/10.1128/aem.70.11.6551-6558.2004.

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ABSTRACT A sensitive NO2 − biosensor that is based on bacterial reduction of NO2 − to N2O and subsequent detection of the N2O by a built-in electrochemical N2O sensor was developed. Four different denitrifying organisms lacking NO3 − reductase activity were assessed for use in the biosensor. The relevant physiological aspects examined included denitrifying characteristics, growth rate, NO2 − tolerance, and temperature and salinity effects on the growth rate. Two organisms were successfully used in the biosensor. The preferred organism was Stenotrophomonas nitritireducens, which is an organism with a denitrifying pathway deficient in both NO3 − and N2O reductases. Alternatively Alcaligenes faecalis could be used when acetylene was added to inhibit its N2O reductase. The macroscale biosensors constructed exhibited a linear NO2 − response at concentrations up to 1 to 2 mM. The detection limit was around 1 μM NO2 −, and the 90% response time was 0.5 to 3 min. The sensor signal was specific for NO2 −, and interference was observed only with NH2OH, NO, N2O, and H2S. The sensor signal was affected by changes in temperature and salinity, and calibration had to be performed in a system with a temperature and an ionic strength comparable to those of the medium analyzed. A broad range of water bodies could be analyzed with the biosensor, including freshwater systems, marine systems, and oxic-anoxic wastewaters. The NO2 − biosensor was successfully used for long-term online monitoring in wastewater. Microscale versions of the NO2 − biosensor were constructed and used to measure NO2 − profiles in marine sediment.
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Murray, Shauna A., Maria Wiese, Anke Stüken, Steve Brett, Ralf Kellmann, Gustaaf Hallegraeff, and Brett A. Neilan. "sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters." Applied and Environmental Microbiology 77, no. 19 (August 12, 2011): 7050–57. http://dx.doi.org/10.1128/aem.05308-11.

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ABSTRACTThe recent identification of genes involved in the production of the potent neurotoxin and keystone metabolite saxitoxin (STX) in marine eukaryotic phytoplankton has allowed us for the first time to develop molecular genetic methods to investigate the chemical ecology of harmful algal bloomsin situ. We present a novel method for detecting and quantifying the potential for STX production in marine environmental samples. Our assay detects a domain of the genesxtAthat encodes a unique enzyme putatively involved in thesxtpathway in marine dinoflagellates,sxtA4. A product of the correct size was recovered from nine strains of four species of STX-producingAlexandriumandGymnodinium catenatumand was not detected in the non-STX-producingAlexandriumspecies, other dinoflagellate cultures, or an environmental sample that did not contain known STX-producing species. However,sxtA4was also detected in the non-STX-producing strain ofAlexandrium tamarense, Tasmanian ribotype. We investigated the copy number ofsxtA4in three strains ofAlexandrium catenellaand found it to be relatively constant among strains. Using our novel method, we detected and quantifiedsxtA4in three environmental blooms ofAlexandrium catenellathat led to STX uptake in oysters. We conclude that this method shows promise as an accurate, fast, and cost-effective means of quantifying the potential for STX production in marine samples and will be useful for biological oceanographic research and harmful algal bloom monitoring.
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Labonté, Jessica M., Karen E. Reid, and Curtis A. Suttle. "Phylogenetic Analysis Indicates Evolutionary Diversity and Environmental Segregation of Marine Podovirus DNA Polymerase Gene Sequences." Applied and Environmental Microbiology 75, no. 11 (April 10, 2009): 3634–40. http://dx.doi.org/10.1128/aem.02317-08.

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ABSTRACT The distribution of viral genotypes in the ocean and their evolutionary relatedness remain poorly constrained. This paper presents data on the genetic diversity and evolutionary relationships of 1.2-kb DNA polymerase (pol) gene fragments from podoviruses. A newly designed set of PCR primers was used to amplify DNA directly from coastal sediment and water samples collected from inlets adjacent to the Strait of Georgia, British Columbia, Canada, and from the northeastern Gulf of Mexico. Restriction fragment length polymorphism analysis of 160 cloned PCR products revealed 29 distinct operational taxonomic units (OTUs), with OTUs within a site typically being more similar than those among sites. Phylogenetic analysis of the DNA pol gene fragments demonstrated high similarity between some environmental sequences and sequences from the marine podoviruses roseophage SIO1 and cyanophage P60, while others were not closely related to sequences from cultured phages. Interrogation of the CAMERA database for sequences from metagenomics data demonstrated that the amplified sequences were representative of the diversity of podovirus pol sequences found in marine samples. Our results indicate high genetic diversity within marine podovirus communities within a small geographic region and demonstrate that the diversity of environmental polymerase gene sequences for podoviruses is far more extensive than previously recognized.
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Dissertations / Theses on the topic "Environmental marine biotechnology"

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Bhedi, Chinmayee D. "Quorum Sensing Signals Produced by Heterotrophic Bacteria in Black Band Disease (BBD) of Corals and Their Potential Role in BBD Pathogenesis." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3367.

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Black band disease (BBD) of corals is a temperature dependent, highly virulent, polymicrobial disease affecting reef-building corals globally. The microbial consortium of BBD is primarily comprised of functional physiological groups that include photosynthetic cyanobacteria, sulfate reducers, sulfide oxidizers and a vast repertoire of heterotrophic bacteria. Quorum sensing (QS), the cell-density dependent communication phenomenon in bacteria, is known to induce expression of genes for a variety of virulence factors in diseases worldwide. Microbes capable of QS release signals such as acyl homoserine lactones (AHLs) and autoinducer-2 (AI-2), which coordinate microbial interaction. The focus of the present study was to investigate the presence and potential role of QS in BBD pathogenicity, utilizing culture dependent and independent methodologies. Isolates across coral health states including BBD, were screened for production of QS signals, and AHL and AI-2 production capabilities were analyzed via LC-MS/MS. The effect of temperature on AHLs was also examined. Additionally, antimicrobial production capabilities of isolates were tested. BBD metagenomes were utilized to screen for sequences related to QS, antimicrobial synthesis, and antimicrobial resistance genes. BBD isolates represented a significantly higher proportion of isolates capable of producing QS signals in comparison to healthy coral isolates. Several AHLs produced by coral derived bacterial cultures were identified, and three AHLs, specifically 3OHC4, 3OHC5 and 3OHC6, showed a significant increase in production at an elevated temperature of 30 °C, which correlates with increased BBD incidence on reefs with increasing water temperature. Most of the BBD cultured isolates were identified as vibrios. Several sequences related to QS, antimicrobial synthesis and resistance genes were detected in the BBD metagenomes. Based on the findings of this study, a model for potential microbial interactions amongst BBD heterotrophs, centered around QS, is proposed. Taken together, the findings from this study provide a clearer understanding of the potential role of QS in BBD, and serve as the basis for further studies aimed at elucidating the pathogenesis of an intricate coral disease.
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Sandström, Victoria. "NanoplastEn litteraturstudie om mänsklig exponering för nanoplast." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-86274.

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År 2015 hade cirka 6 300 miljoner ton plastavfall genererats globalt, varav 79% ansamlades på deponier eller i den naturliga miljön. I dagsläget återfinns plastskräp i både terrestra och marina miljöer och större delen av plastavfallet är persistent. Då naturen inte kan bryta ned plasten sönderdelas den istället till mikroplast (<5 000 µm) och sedan till nanoplast (1–1 000 nm). I denna litteraturstudie undersöktes människors exponering för nanoplast, vilka marina livsmedel som kan innehålla nanoplast samt nanoplastens påverkan på mänskliga celler. Resultaten visar att människor riskerar att exponeras för nanoplast genom luften, huden samt vid konsumtion av marina livsmedel såsom fisk, musslor och ostron. I experimentella miljöer visar resultaten att mänskliga celler tar upp nanoplastpartiklar av polystyren i olika storlekar, varav mindre partiklar internaliseras snabbare och i större mängd. Även ytladdningen kan ha en påverkan på mänskliga celler där aminomodifierade (PS-NH2) samt karboxylmodifierade (PS-COOH) visade högre cytotoxicitet än polystyrenpartiklar utan modifierad yta (PS-NP). Vidare indikerar resultaten att höga doser av polystyrenpartiklar reducerar cellvibiliteten samt inducerar morfologiska förändringar och oxidativ stress i cellerna. I dagsläget är det oklart i vilken mängd nanoplast återfinns i miljön, därav är det svårt att beräkna den totala exponeringen som människor kan utsättas för. I experimentella miljöer utsätts cellerna för höga doser under kort tid, medan den verkliga mänskliga exponeringen troligtvis sker med små doser under lång tid.
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Al, Mallah Maha. "Biodegradation des hydrocarbures dans les milieux sursales." Aix-Marseille 2, 1988. http://www.theses.fr/1988AIX22040.

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(9878000), SJ Fox. "A conceptual framework for integrated coastal management in Australia." Thesis, 2009. https://figshare.com/articles/thesis/A_conceptual_framework_for_integrated_coastal_management_in_Australia/13433555.

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Books on the topic "Environmental marine biotechnology"

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United States. Agricultural Biotechnology Research Advisory Committee. Working Group on Aquatic Biotechnology and Environmental Safety. Minutes, Agricultural Biotechnology Research Advisory Committee, Working Group on Aquatic Biotechnology and Environmental Safety: November 17, 1994. Washington, D.C.?]: U.S. Dept. of Agriculture, Office of Agricultural Biotechnology, 1994.

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United States. Agricultural Biotechnology Research Advisory Committee. Working Group on Aquatic Biotechnology and Environmental Safety. Minutes, Agricultural Biotechnology Research Advisory Committee, Working Group on Aquatic Biotechnology and Environmental Safety: October 15, 1992. Washington, D.C.]: U.S. Dept. of Agriculture, Office of Agricultural Biotechnology, 1992.

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Tebo, Bradley MacLean. Environmental applications of marine biotechnology: Summary of a Program Development Workshop sponsored by the California Sea Grant College System. La Jolla, Calif: California Sea Grant College System, University of California, 1995.

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A, Zilinskas Raymond, and Balint Peter J. 1950-, eds. Genetically engineered marine organisms: Environmental and economic risks and benefits. Boston: Kluwer Academic Publishers, 1998.

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1943-, Fusetani Nobuhiro, and Clare Anthony S, eds. Antifouling compounds. Berlin: Springer, 2006.

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Environment, United States Congress House Committee on Merchant Marine and Fisheries Subcommittee on Fisheries and Wildlife Conservation and the. Opportunities for growth in marine aquaculture and marine biotechnology industries: Hearing before the Subcommittee on Fisheries and Wildlife Conservation and the Environment of the Committee on Merchant Marine and Fisheries, House of Representatives, One Hundred Second Congress, second session ... September 22, 1992. Washington: U.S. G.P.O., 1992.

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United States. Congress. House. Committee on Science, Space, and Technology. Subcommittee on Natural Resources, Agriculture Research, and Environment. Future application of aquaculture and marine biotechnology to the nation's estuaries and coastal waters: Hearing before the Subcommittee on Natural Resources, Agriculture Research, and Environment of the Committee on Science, Space, and Technology, U.S. House of Representatives, One Hundred First Congress, first session, May 15, 1989. Washington: U.S. G.P.O., 1989.

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Thompson, Mary-Frances, and Rachakonda Nagabhushanam. Environmental Marine Biotechnology (Recent Advances in Marine Biotechnology , Vol 2). Science Publishers, 1999.

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Opportunities for Environmental Applications of Marine Biotechnology. Washington, D.C.: National Academies Press, 2000. http://dx.doi.org/10.17226/9988.

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(US), National Research Council, Board on Biology, and Oceans Studies Board. Opportunities for Environmental Application of Marine Biotechnology. National Academies Press, 2000.

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Book chapters on the topic "Environmental marine biotechnology"

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Healy, M. G., R. O. Bustos, S. E. Solomon, C. Devine, and A. Healy. "Biotreatment of Marine Crustacean and Chicken Egg Shell Waste." In Environmental Biotechnology, 302–19. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-017-1435-8_27.

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Deepika, K. V., G. Mohana Sheela, and Pallaval Veera Bramhachari. "Marine Microbial Biosurfactants: Ecological and Environmental Applications." In Environmental Biotechnology Vol. 3, 221–32. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-48973-1_8.

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Garcés-Fiallos, Felipe R., Felipe M. de Quadros, and Mateus B. de Freitas. "Marine Resources with Potential in Controlling Plant Diseases." In Environmental and Microbial Biotechnology, 703–19. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8999-7_24.

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Pabbathi, Ninian Prem Prashanth, Neelam M. Nathani, Indra Ramjibhai Gadhvi, and Mootapally Chandrashekar. "Environmental Metabolomics: With the Perspective of Marine Toxicology Assessment." In Environmental Biotechnology Vol. 1, 197–225. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-38192-9_8.

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Hossain, Jakir, and Roksana Jahan. "Biofuel: Marine Biotechnology Securing Alternative Sources of Renewable Energy." In Environmental and Microbial Biotechnology, 161–94. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8999-7_7.

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Kavitha, G., and D. Inbakanadan. "Ecofriendly Synthesis of Biopolymer Nanocomposites and Its Application as a Potent Marine Antifouling Agent." In Environmental Biotechnology Vol. 1, 181–95. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-38192-9_7.

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Bulgariu, Laura, and Dumitru Bulgariu. "Sustainable Utilization of Marine Algae Biomass for Environmental Bioremediation." In Prospects and Challenges in Algal Biotechnology, 179–217. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-1950-0_6.

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Olanrewaju, Oladokun Sulaiman, Giuseppina Tommonaro, Giulia Guerriero, Chiara Fogliano, Carmine Iodice, Gennaro Velotto, and Annabella Tramice. "New Insight into Marine Biotechnology: Carrageenans Chemical Features and Acetylcholinesterase (AChE) Inhibition Activity of Two Edible Seaweeds of the Genus Kappaphycus." In Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition), 2203–7. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-51210-1_345.

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Lozada, Mariana, and Hebe M. Dionisi. "Microbial Bioprospecting in Marine Environments." In Hb25_Springer Handbook of Marine Biotechnology, 307–26. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-53971-8_11.

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Vaksmaa, Annika, Victor Hernando-Morales, Emna Zeghal, and Helge Niemann. "Microbial Degradation of Marine Plastics: Current State and Future Prospects." In Biotechnology for Sustainable Environment, 111–54. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-1955-7_5.

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Conference papers on the topic "Environmental marine biotechnology"

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Hosoda, Ryusuke, Shigemochi Murota, Masahiko Yao, and Yuki Tamura. "ECO-PLATFORM: A Solution for Solving Environmental Problems in Coastal Area." In ASME 2005 24th International Conference on Offshore Mechanics and Arctic Engineering. ASMEDC, 2005. http://dx.doi.org/10.1115/omae2005-67296.

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The authors proposed a concept of “ECO-PLATFORM” for solving environmental problems that people have been facing and will face in and around the coastal area, setting their sights on the contribution from the viewpoint of the sea [1]. The Eco-Platform is a complex on a floating platform and has functions for solving social and environmental problems. The functions to be installed are generation of heat energy and electric power from combustible waste without introducing fossil energy, cascade use of heat energy, application of marine biotechnology for processing the sewage and for purifying eutrophic seawater, reproduction/regeneration of material and energy resources from manmade waste and sewage. All the functions to be installed are integrated systematically and efficiently. To ensure the realization of the concept, it is necessary to study/investigate the feasibility of systematic combination of functions and facilities installed on the Eco-Platform. The authors have been studying the required functions and size of each facility, probable waste/sewage/energy/resource input and output relations. After the studies on the desirable combination and arrangement of installed facilities, they also studied the energy and material balance of the total system from the technical standpoints to implement the Eco-Platform system. They will show as the results of the study that the Eco-Platform is technically feasible introducing technologies mainly developed in the latter half of the 20th century and will show possible combination of functions/facilities supposing waste/sewage disposal in Sakai-city, Osaka, Japan.
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Yang, Dongfang, Yu Chen, Xiancheng Qu, Danfeng Yang, and Yinjiang Zhang. "Seasonal-vertical variations of Pb and their mechanisms in marine bay." In 2016 6th International Conference on Machinery, Materials, Environment, Biotechnology and Computer. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/mmebc-16.2016.396.

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Reports on the topic "Environmental marine biotechnology"

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Robinson, Larry. Marine Biotechnology and Marine Environmental Science Research Program. Office of Scientific and Technical Information (OSTI), January 2003. http://dx.doi.org/10.2172/900248.

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Sowers, Kevin R. Graduate and Undergraduate Training in Marine Environmental Biotechnology. Fort Belvoir, VA: Defense Technical Information Center, November 2002. http://dx.doi.org/10.21236/ada408279.

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