Relevant bibliographies by topics / Enzymatic bioremediation / Journal articles
To see the other types of publications on this topic, follow the link: Enzymatic bioremediation.

Journal articles on the topic 'Enzymatic bioremediation'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Enzymatic bioremediation.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Scott, Colin, Gunjan Pandey, Carol J. Hartley, Colin J. Jackson, Matthew J. Cheesman, Matthew C. Taylor, Rinku Pandey, et al. "The enzymatic basis for pesticide bioremediation." Indian Journal of Microbiology 48, no. 1 (March 2008): 65–79. http://dx.doi.org/10.1007/s12088-008-0007-4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Sutherland, TD, I. Horne, KM Weir, CW Coppin, MR Williams, M. Selleck, RJ Russell, and JG Oakeshott. "ENZYMATIC BIOREMEDIATION: FROM ENZYME DISCOVERY TO APPLICATIONS." Clinical and Experimental Pharmacology and Physiology 31, no. 11 (November 2004): 817–21. http://dx.doi.org/10.1111/j.1440-1681.2004.04088.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Ruggaber, Timothy P., and Jeffrey W. Talley. "Enhancing Bioremediation with Enzymatic Processes: A Review." Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management 10, no. 2 (April 2006): 73–85. http://dx.doi.org/10.1061/(asce)1090-025x(2006)10:2(73).

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Lovley, Derek R., and Elizabeth J. P. Phillips. "Bioremediation of uranium contamination with enzymatic uranium reduction." Environmental Science & Technology 26, no. 11 (November 1992): 2228–34. http://dx.doi.org/10.1021/es00035a023.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Cheriyan, Soly, and Emilia T. Abraham. "Enzymatic bioremediation of cashew nut shell liquid contamination." Journal of Hazardous Materials 176, no. 1-3 (April 15, 2010): 1097–100. http://dx.doi.org/10.1016/j.jhazmat.2009.11.091.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Kapoor, Mukesh, and R. Rajagopal. "Enzymatic bioremediation of organophosphorus insecticides by recombinant organophosphorous hydrolase." International Biodeterioration & Biodegradation 65, no. 6 (September 2011): 896–901. http://dx.doi.org/10.1016/j.ibiod.2010.12.017.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Semenenko, S. Y., and N. V. Morozova. "Salinity alterations in response to soil bioremediation by enzymatic biostimulation." Agrarian Scientific Journal, no. 1 (January 19, 2018): 35–38. http://dx.doi.org/10.28983/asj.v0i1.325.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Jiménez-T, R. Gómez, E. Moliterni, L. Rodríguez, F. J. Fernández, and J. Villaseñor. "Feasibility of mixed enzymatic complexes to enhanced soil bioremediation processes." Procedia Environmental Sciences 9 (2011): 54–59. http://dx.doi.org/10.1016/j.proenv.2011.11.010.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Rocha, George Jackson de Moraes, Viviane Marcos Nascimento, and Vinicius Fernandes Nunes da Silva. "Enzymatic Bioremediation of Effluent from Sugarcane Bagasse Soda Delignification Process." Waste and Biomass Valorization 5, no. 6 (July 19, 2014): 919–29. http://dx.doi.org/10.1007/s12649-014-9316-5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Ávila-Pozo, Paloma, Juan Parrado, Pablo Caballero, Marta Díaz-López, Felipe Bastida, and Manuel Tejada. "Use of Slaughterhouse Sludge in the Bioremediation of an Oxyfluorfen-Polluted Soil." International Journal of Environmental Research 15, no. 4 (June 25, 2021): 723–31. http://dx.doi.org/10.1007/s41742-021-00351-z.

Full text
Abstract:
AbstractThe use of organic matter is a highly accepted environmental practice among scientists for the bioremediation of polluted soils. In this manuscript we study under laboratory conditions the bioremediation capacity of a new biostimulant obtained from slaughterhouse sludge in a soil polluted by the oxyfluorfen at a rate of 4 l ha−1 (manufacturer’s rate recommended) over a 90-day period. We determined its effects on dehydrogenase, urease, β-glucosidase and phosphatase activities, the soil microbial community structure and the evolution of the herbicide in soil. Possibly due to the high content of low molecular weight proteins in the biostimulant, the enzymatic activities were stimulated mainly at the beginning of the experiment. Soil biological parameters were inhibited in oxyfluorfen-polluted soil. At the end of the experiment and compared with the control soil, dehydrogenase, urease, β-glucosidase, and phosphatase activities significantly decreased by 47.8%, 50.5%, 36.4%, and 45.5% in the oxyfluorfen-polluted soil. At 5 days into the experiment, the use of the biostimulant in oxyfluorfen-polluted soils decreased soil enzymatic activities and microbial community inhibition. At the end of the incubation period the oxyfluorfen concentration had decreased by 60% in the polluted soil and amended with biostimulants. These results suggested that the use of this biostimulant with higher amounts of low molecular weight proteins and peptides had a positive effect on the remediating oxyfluorfen-polluted soils. Therefore, this study provides the use of a new biostimulant obtained from slaughterhouse sludge by enzymatic hydrolysis processes used in the bioremediation of a soil polluted by the oxyfluorfen herbicide.
APA, Harvard, Vancouver, ISO, and other styles
11

Peixoto, R. S., A. B. Vermelho, and A. S. Rosado. "Petroleum-Degrading Enzymes: Bioremediation and New Prospects." Enzyme Research 2011 (July 24, 2011): 1–7. http://dx.doi.org/10.4061/2011/475193.

Full text
Abstract:
Anthropogenic forces, such as petroleum spills and the incomplete combustion of fossil fuels, have caused an accumulation of petroleum hydrocarbons in the environment. The accumulation of petroleum and its derivatives now constitutes an important environmental problem. Biocatalysis introduces new ways to improve the development of bioremediation strategies. The recent application of molecular tools to biocatalysis may improve bioprospecting research, enzyme yield recovery, and enzyme specificity, thus increasing cost-benefit ratios. Enzymatic remediation is a valuable alternative as it can be easier to work with than whole organisms, especially in extreme environments. Furthermore, the use of free enzymes avoids the release of exotic or genetically modified organisms (GMO) in the environment.
APA, Harvard, Vancouver, ISO, and other styles
12

Sanchez-Hernandez, Juan C., Ximena Andrade Cares, and Jorge Domínguez. "Exploring the potential enzymatic bioremediation of vermicompost through pesticide-detoxifying carboxylesterases." Ecotoxicology and Environmental Safety 183 (November 2019): 109586. http://dx.doi.org/10.1016/j.ecoenv.2019.109586.

Full text
APA, Harvard, Vancouver, ISO, and other styles
13

Shukla, Awadhesh Kumar, and Amit Kishore Singh. "Exploitation of Potential Extremophiles for Bioremediation of Xenobiotics Compounds: A Biotechnological Approach." Current Genomics 21, no. 3 (July 10, 2020): 161–67. http://dx.doi.org/10.2174/1389202921999200422122253.

Full text
Abstract:
Microorganisms that are capable of live and adapt in hostile habitats of different environmental factors such as extremes temperature, salinity, nutrient availability and pressure are known as extremophiles. Exposure to xenobiotic compounds is global concern influencing the world population as a health hazard. Hence their removal is warranted using biological means that is very sustainable, potentially cost-effective and eco-friendly. Due to adaptation in extreme environments and unique defense mechanisms, they are receiving more attention for the bioremediation of the xenobiotic compounds. They possess robust enzymatic and biocatalytic systems that make them suitable for the effective removal of pollutants from the contaminated environment. Additionally, the extremophiles act as microfactories having specific genetic and biotechnological potential for the production of biomolecules. This mini review will provide an overview of microbial degradation metabolic pathways for bioremediation along with the molecular and physiological properties of diverse extremophiles from variety of habitats. Furthermore, the factors affecting the bioremediation process is also summarized.
APA, Harvard, Vancouver, ISO, and other styles
14

Wołejko, Elżbieta, Urszula Wydro, and Tadeusz Łoboda. "The ways to increase efficiency of soil bioremediation." Ecological Chemistry and Engineering S 23, no. 1 (March 1, 2016): 155–74. http://dx.doi.org/10.1515/eces-2016-0011.

Full text
Abstract:
Abstract The aim of this paper was to present possibilities of using different substrates to assist the bioremediation of soils contaminated with heavy metals, pesticides and other substances. Today's bioengineering offers many solutions that enable the effective conduct of biological remediation, including both biostimulation and bioaugmentation. For this purpose, they are used to enrich various organic substances, sorbents, microbiological and enzymatic preparations, chemical substances of natural origin or nanoparticles. The use of genetic engineering as a tool to obtain microorganisms and plants capable of efficient degradation of pollutants may cause the risks that entails the introduction of transgenic plants and microorganisms into the environment. In order to determine the efficacy and possible effects of the various bioremediation techniques, it is required to conduct many studies and projects on a larger scale than only in the laboratory. Furthermore, it should be emphasized that bioremediation involves interdisciplinary issues and therefore, there is a need to combine knowledge from different disciplines, such as: microbiology, biochemistry, ecology, environmental engineering and process engineering.
APA, Harvard, Vancouver, ISO, and other styles
15

Viswanath, Buddolla, Bandi Rajesh, Avilala Janardhan, Arthala Praveen Kumar, and Golla Narasimha. "Fungal Laccases and Their Applications in Bioremediation." Enzyme Research 2014 (May 15, 2014): 1–21. http://dx.doi.org/10.1155/2014/163242.

Full text
Abstract:
Laccases are blue multicopper oxidases, which catalyze the monoelectronic oxidation of a broad spectrum of substrates, for example, ortho- and para-diphenols, polyphenols, aminophenols, and aromatic or aliphatic amines, coupled with a full, four-electron reduction of O2 to H2O. Hence, they are capable of degrading lignin and are present abundantly in many white-rot fungi. Laccases decolorize and detoxify the industrial effluents and help in wastewater treatment. They act on both phenolic and nonphenolic lignin-related compounds as well as highly recalcitrant environmental pollutants, and they can be effectively used in paper and pulp industries, textile industries, xenobiotic degradation, and bioremediation and act as biosensors. Recently, laccase has been applied to nanobiotechnology, which is an increasing research field, and catalyzes electron transfer reactions without additional cofactors. Several techniques have been developed for the immobilization of biomolecule such as micropatterning, self-assembled monolayer, and layer-by-layer techniques, which immobilize laccase and preserve their enzymatic activity. In this review, we describe the fungal source of laccases and their application in environment protection.
APA, Harvard, Vancouver, ISO, and other styles
16

Dias, João CT, Rachel P. Rezende, Carlos A. Rosa, Marc-André Lachance, and Valter R. Linardi. "Enzymatic degradation of nitriles by aCandida guilliermondiiUFMG-Y65." Canadian Journal of Microbiology 46, no. 6 (June 1, 2000): 525–31. http://dx.doi.org/10.1139/w00-027.

Full text
Abstract:
Candida guilliermondii UFMG-Y65, isolated from a gold mine, was able to utilize different nitriles and the corresponding amides as sole source of nitrogen, at concentrations up to 2 M. Resting cells cultivated on YCB-acetonitrile medium showed nitrile hydrolyzing enzyme activities against acrylonitrile and benzonitrile. These enzymes were inducible and intracellular; the optimum pH was 7.0-8.0, and the optimum temperature 25°C-30°C. Liquid chromatographic analysis indicated that C. guilliermondii UFMG-Y65 metabolized 12 mM benzonitrile to 11 mM benzoic acid and 10 mM acrylonitrile to 7.9 mM acrylic acid. The results suggest that C. guilliermondii UFMG-Y65 may be useful for the bioproduction of amides and acids, and for the bioremediation of environments contaminated with nitriles.Key words: Candida guilliermondii, nitrile hydrolyzing enzyme, amidase, nitriles, amides.
APA, Harvard, Vancouver, ISO, and other styles
17

Marchut-Mikolajczyk, O. "Enzymatic preparation from Mucor racemosus enhancing bioremediation of soil contaminated with petroleum hydrocarbons." New Biotechnology 25 (September 2009): S277. http://dx.doi.org/10.1016/j.nbt.2009.06.625.

Full text
APA, Harvard, Vancouver, ISO, and other styles
18

Dixit, Mandeep, Guddu Kumar Gupta, Zeba Usmani, Minaxi Sharma, and Pratyoosh Shukla. "Enhanced bioremediation of pulp effluents through improved enzymatic treatment strategies: A greener approach." Renewable and Sustainable Energy Reviews 152 (December 2021): 111664. http://dx.doi.org/10.1016/j.rser.2021.111664.

Full text
APA, Harvard, Vancouver, ISO, and other styles
19

Singh, M. P., S. K. Vishwakarma, and A. K. Srivastava. "Bioremediation of Direct Blue 14 and Extracellular Ligninolytic Enzyme Production by White Rot Fungi:PleurotusSpp." BioMed Research International 2013 (2013): 1–4. http://dx.doi.org/10.1155/2013/180156.

Full text
Abstract:
In the present investigation, four species of white rot fungi (Pleurotus), that is,P. flabellatus, P. florida, P. ostreatusandP. sajor-cajuwere used for decolorization of direct blue 14 (DB14). Among all four species ofPleurotus,P. flabellatusshowed the fastest decolorization in petri plates on different concentration, that is, 200 mg/L, 400 mg/L, and 600 mg/L. All these four species were also evaluated for extracellular ligninolytic enzymes (laccase and manganese peroxidase) production and it was observed that the twelve days old culture ofP. flabellatusshowed the maximum enzymatic activity, that is, 915.7 U/mL and 769.2 U/mL of laccase and manganese peroxidase, respectively. Other threePleurotusspecies took more time for dye decolorization and exhibited less enzymatic activities. The rate of decolorization of DB14 dye solution (20 mg/L) by crude enzymes isolated fromP. flabellatuswas very fast, and it was observed that up to 90.39% dye solution was decolorized in 6 hrs of incubation.
APA, Harvard, Vancouver, ISO, and other styles
20

Nguyen, Luong N., Minh T. Vu, Md Abu Hasan Johir, Nirenkumar Pathak, Jakub Zdarta, Teofil Jesionowski, Galilee U. Semblante, Faisal I. Hai, Hong Khanh Dieu Nguyen, and Long D. Nghiem. "A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation." Processes 8, no. 6 (May 29, 2020): 648. http://dx.doi.org/10.3390/pr8060648.

Full text
Abstract:
Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.
APA, Harvard, Vancouver, ISO, and other styles
21

Germain, Joaquim, Muriel Raveton, Marie-Noëlle Binet, and Bello Mouhamadou. "Potentiality of Native Ascomycete Strains in Bioremediation of Highly Polychlorinated Biphenyl Contaminated Soils." Microorganisms 9, no. 3 (March 16, 2021): 612. http://dx.doi.org/10.3390/microorganisms9030612.

Full text
Abstract:
Polychlorinated biphenyls (PCBs) are organic pollutants that are harmful to environment and toxic to humans. Numerous studies, based on basidiomycete strains, have reported unsatisfactory results in the mycoremediation of PCB-contaminated soils mainly due to the non-telluric origin of these strains. The abilities of a five-Ascomycete-strain consortium in the mycoremediation of PCB-polluted soils and its performance to restore their sound functioning were investigated using mesocosm experiments associated with chromatography gas analysis and enzymatic activity assays. With the soil H containing 850 ppm PCB from which the strains had been isolated, a significant PCB depletion of 29% after three months of treatment was obtained. This led to an important decrease of PCBs from 850 to 604 ppm. With the soil L containing 36 ppm PCB, biodegradation did not occur. In both soils, the fungal biomass quantified by the ergosterol assay, did not increase at the end of the treatment. Biodegradation evidenced in the soil H resulted in a significantly improved stoichiometry of N and P acquiring enzymatic activities. This unprecedented study demonstrates that the native Ascomycetes display remarkable properties for remediation and restoration of functioning of the soil they originated from paving the way for greater consideration of these strains in mycoremediation.
APA, Harvard, Vancouver, ISO, and other styles
22

Asgher, Muhammad, Qamar Yasmeen, and Hafiz Muhammad Nasir Iqbal. "Development of novel enzymatic bioremediation process for textile industry effluents through response surface methodology." Ecological Engineering 63 (February 2014): 1–11. http://dx.doi.org/10.1016/j.ecoleng.2013.09.042.

Full text
APA, Harvard, Vancouver, ISO, and other styles
23

Kumar, Mohit, Kushneet Kaur Sodhi, and Dileep Kumar Singh. "Bioremediation of Penicillin G by Serratia sp. R1, and enzymatic study through molecular docking." Environmental Nanotechnology, Monitoring & Management 12 (December 2019): 100246. http://dx.doi.org/10.1016/j.enmm.2019.100246.

Full text
APA, Harvard, Vancouver, ISO, and other styles
24

Gospodarek, Janina, Milena Rusin, Gabriela Barczyk, and Aleksandra Nadgórska-Socha. "The Effect of Petroleum-Derived Substances and Their Bioremediation on Soil Enzymatic Activity and Soil Invertebrates." Agronomy 11, no. 1 (January 2, 2021): 80. http://dx.doi.org/10.3390/agronomy11010080.

Full text
Abstract:
Petroleum-derived substances (PDSs) as main pollutants of the natural environment can negatively affect the microbiological, biochemical, and biological properties of agricultural soils and, consequently, plant production. The present study aimed to determine the after-effect of PDSs such as petrol, used engine oil, and diesel fuel on the activity of selected soil enzymes (phosphatase, dehydrogenase, and urease) and on the occurrence of soil invertebrates. Moreover, changes in the analyzed parameters in response to bioremediation of the polluted soil by using ZB-01 preparation were investigated. The field experiments were performed four- and five-years post contamination. The results showed that even after five years, PDSs significantly modified the activity of soil enzymes; however, this effect was often varied, depending on the pollutant, enzyme, and time after soil contamination. Dehydrogenase seems to be a good indicator of soil contamination with PDSs, particularly diesel fuel. Engine oil and diesel fuel limited still the occurrence of soil invertebrates, particularly Collembola from the families Hypogastruidae, Isotomidae, and Entomobryidae, even after four and five years of contamination. This finding suggests the usefulness of these organisms in assessing soil pollution and in monitoring the progress of bioremediation. The effect of ZB-01 biopreparation on the activity of selected enzymes was varied. Its effect on the occurrence of soil invertebrates was usually beneficial, which was evident in diesel fuel-contaminated soil.
APA, Harvard, Vancouver, ISO, and other styles
25

Ianieva, O. D. "Halotolerant Yeasts: Biodiversity and Potential Application." Mikrobiolohichnyi Zhurnal 82, no. 5 (October 17, 2020): 65–87. http://dx.doi.org/10.15407/microbiolj82.05.065.

Full text
Abstract:
Halotolerant yeasts represent a heterogeneous group of unicellular fungi able to survive and thrive under hypersaline conditions. This review examines the biodiversity of halotolerant yeasts in various habitats with high salt content and the potential practical applications of this group of microorganisms in industry and agriculture. Halotolerant yeasts are found in various habitats with elevated salt content, including seawater, hypersaline ponds and salterns, saline soils and wastewaters, salt-containing foods. Habitats with moderate salinity, e.g. seawater, food products, olive fermentation wastewaters can boast a comparatively large biodiversity of yeasts both ascomycetes and basidiomycetes. Hypersaline niches are mostly inhabited by pigmented and melanized yeasts and yeast-like fungi. The adaptability and robustness of halotolerant yeasts could be exploited in several biotechnological fields, mainly the food industry and bioremediation. Yeasts isolated from food products with elevated salt content are studied as potential starter cultures in the corresponding fermenting products due to their enzymatic and antimicrobial activity and probiotic characteristics. Marine yeasts are of an increasing interest due to their production of various hydrolytic enzymes, biofuel production using seawater, bioremediation of saline wastewaters and the probiotic potential in aquaculture. Halotolerant yeasts found in various saline wastewaters could be used in bioremediation of wastewaters with high salinity containing various organic pollutants. However more research is required to achieve practical utilization of this group of microorganisms.
APA, Harvard, Vancouver, ISO, and other styles
26

Dunoyer, Arnulfo Tarón, Rafael Emilio González Cuello, and Rosangela Perez Salinas. "Biodegradation of dairy wastes using crude enzymatic extract of Yarrowia lipolytica ATCC 9773." Ambiente e Agua - An Interdisciplinary Journal of Applied Science 15, no. 1 (February 14, 2020): 1. http://dx.doi.org/10.4136/ambi-agua.2448.

Full text
Abstract:
Effluents generated by the food industry have become a serious environmental concern. Bioremediation is a biological process developed as an alternative for the treatment of contaminated areas. In current research, the biodegradation of fat, Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD) and total solids were evaluated in dairy waste employing enzymatic extract of Yarrowia lipolytica ATCC 9773 as biological agents. All the variables were determined following the specifications of the Standard Methods of the American Water Works Association. Enzymatic extract of Y. lipolytica at different concentrations (8, 12 and 16.0%) was used in a fermentative medium at two pHs (5.0 and 6.5) for 32 h. The highest percentages (%) of fat (82.88), BOD (43.32), COD (44.3) and total solids (13.58) removal were obtained using an inoculum concentration of 16% at pH 5.0 for 32 h of fermentation. These results may have industrial relevance for the reduction of contamination of industrial effluents with high levels of fat and other contaminants.
APA, Harvard, Vancouver, ISO, and other styles
27

Arriel Torres, Juliana, Pricila Maria Batista Chagas, Maria Cristina Silva, Custódio Donizete dos Santos, and Angelita Duarte Corrêa. "Enzymatic oxidation of phenolic compounds in coffee processing wastewater." Water Science and Technology 73, no. 1 (September 4, 2015): 39–50. http://dx.doi.org/10.2166/wst.2015.332.

Full text
Abstract:
Peroxidases can be used in the treatment of wastewater containing phenolic compounds. The effluent from the wet processing of coffee fruits contains high content of these pollutants and although some studies propose treatments for this wastewater, none targets specifically the removal of these recalcitrant compounds. This study evaluates the potential use of different peroxidase sources in the oxidation of caffeic acid and of total phenolic compounds in coffee processing wastewater (CPW). The identification and quantification of phenolic compounds in CPW was performed and caffeic acid was found to be the major phenolic compound. Some factors, such as reaction time, pH, amount of H2O2 and enzyme were evaluated, in order to determine the optimum conditions for the enzyme performance for maximum oxidation of caffeic acid. The turnip peroxidase (TPE) proved efficient in the removal of caffeic acid, reaching an oxidation of 51.05% in just 15 minutes of reaction. However, in the bioremediation of the CPW, the horseradish peroxidase (HRP) was more efficient with 32.70% ± 0.16 of oxidation, followed by TPE with 18.25% ± 0.11. The treatment proposed in this work has potential as a complementary technology, since the efficiency of the existing process is intimately conditioned to the presence of these pollutants.
APA, Harvard, Vancouver, ISO, and other styles
28

Cilerdzic, Jasmina, Jelena Vukojevic, and Mirjana Stajic. "Dynamics of ligninolytic enzyme production in Ganoderma applanatum depending on cultivation type." Zbornik Matice srpske za prirodne nauke, no. 120 (2011): 327–31. http://dx.doi.org/10.2298/zmspn1120327c.

Full text
Abstract:
Ganoderma applanatum belongs to the group of white-rot fungi, due to a well-developed ligninolytic enzyme system. White-rot fungi have attracted great scientific attention in recent years, especially with respect to their enzymatic potential for the bioremediation of persistent pollutants. Contrary to G. lucidum, which medicinal properties, as well as ligninolytic enzyme system have been extensively studied, enzymatic system of G. applanatum has not been studied yet. Thus, the aim of this study was to analyze the dynamics of laccase, Mn-dependent peroxidase, and versatile peroxidase activity during submerged and solid state cultivation on two selected plant raw materials. Enzyme activity was determined spectrophotometrically after 7, 10 and 14 days of cultivation. The peak of laccase activity (220.14 Ul-1) was noted after 14 days of submerged wheat straw fermentation. Maximum level of Mn-dependent peroxidase (110.91 Ul-1) and versatile peroxidase (116.20 Ul-1) activity was obtained in the medium with oak sawdust after 14 days of submerged cultivation.
APA, Harvard, Vancouver, ISO, and other styles
29

Banach, Artur, Agnieszka Kuźniar, Anna Marzec-Grządziel, Anna Gałązka, and Agnieszka Wolińska. "Phenotype Switching in Metal-Tolerant Bacteria Isolated from a Hyperaccumulator Plant." Biology 10, no. 9 (September 7, 2021): 879. http://dx.doi.org/10.3390/biology10090879.

Full text
Abstract:
As an adaptation to unfavorable conditions, microorganisms may represent different phenotypes. Azolla filiculoides L. is a hyperaccumulator of pollutants, but the functions of its microbiome have not been well recognized to date. We aimed to reveal the potential of the microbiome for degradation of organic compounds, as well as its potential to promote plant growth in the presence of heavy metals. We applied the BiologTM Phenotypic Microarrays platform to study the potential of the microbiome for the degradation of 96 carbon compounds and stress factors and assayed the hydrolytic potential and auxin production by the microorganisms in the presence of Pb, Cd, Cr (VI), Ni, Ag, and Au. We found various phenotype changes depending on the stress factor, suggesting a possible dual function of the studied microorganisms, i.e., in bioremediation and as a biofertilizer for plant growth promotion. Delftia sp., Staphylococcus sp. and Microbacterium sp. exhibited high efficacy in metabolizing organic compounds. Delftia sp., Achromobacter sp. and Agrobacterium sp. were efficient in enzymatic responses and were characterized by metal tolerant. Since each strain exhibited individual phenotype changes due to the studied stresses, they may all be beneficial as both biofertilizers and bioremediation agents, especially when combined in one biopreparation.
APA, Harvard, Vancouver, ISO, and other styles
30

SADIQ, S., M. MAHMOOD-UL-HASSAN, S. SABA, K. AHAD, J. ASAD, R. T. MEHMOOD, and T. TAJ. "ENZYMATIC BIOREMEDIATION OF ENDOSULFAN IN SOIL USING LIGNINOLYTIC EXTRACT OF SPENT MUSHROOM COMPOST OF PLEUROTUS OSTREATUS." Applied Ecology and Environmental Research 17, no. 2 (2019): 3251–67. http://dx.doi.org/10.15666/aeer/1702_32513267.

Full text
APA, Harvard, Vancouver, ISO, and other styles
31

Waghmare, Pankajkumar R., Avinash A. Kadam, Ganesh D. Saratale, and Sanjay P. Govindwar. "Enzymatic hydrolysis and characterization of waste lignocellulosic biomass produced after dye bioremediation under solid state fermentation." Bioresource Technology 168 (September 2014): 136–41. http://dx.doi.org/10.1016/j.biortech.2014.02.099.

Full text
APA, Harvard, Vancouver, ISO, and other styles
32

Raffa, Carla Maria, and Fulvia Chiampo. "Bioremediation of Agricultural Soils Polluted with Pesticides: A Review." Bioengineering 8, no. 7 (July 2, 2021): 92. http://dx.doi.org/10.3390/bioengineering8070092.

Full text
Abstract:
Pesticides are chemical compounds used to eliminate pests; among them, herbicides are compounds particularly toxic to weeds, and this property is exploited to protect the crops from unwanted plants. Pesticides are used to protect and maximize the yield and quality of crops. The excessive use of these chemicals and their persistence in the environment have generated serious problems, namely pollution of soil, water, and, to a lower extent, air, causing harmful effects to the ecosystem and along the food chain. About soil pollution, the residual concentration of pesticides is often over the limits allowed by the regulations. Where this occurs, the challenge is to reduce the amount of these chemicals and obtain agricultural soils suitable for growing ecofriendly crops. The microbial metabolism of indigenous microorganisms can be exploited for degradation since bioremediation is an ecofriendly, cost-effective, rather efficient method compared to the physical and chemical ones. Several biodegradation techniques are available, based on bacterial, fungal, or enzymatic degradation. The removal efficiencies of these processes depend on the type of pollutant and the chemical and physical conditions of the soil. The regulation on the use of pesticides is strictly connected to their environmental impacts. Nowadays, every country can adopt regulations to restrict the consumption of pesticides, prohibit the most harmful ones, and define the admissible concentrations in the soil. However, this variability implies that each country has a different perception of the toxicology of these compounds, inducing different market values of the grown crops. This review aims to give a picture of the bioremediation of soils polluted with commercial pesticides, considering the features that characterize the main and most used ones, namely their classification and their toxicity, together with some elements of legislation into force around the world.
APA, Harvard, Vancouver, ISO, and other styles
33

Ayla, Sridevi, Monika Kallubai, Suvarnalatha Devi Pallipati, and Golla Narasimha. "Enzymatic Textile Dyes Decolorization by In vitro and In silico Studies." Recent Patents on Biotechnology 13, no. 4 (October 25, 2019): 268–76. http://dx.doi.org/10.2174/1872208313666190625123847.

Full text
Abstract:
Background:Laccase, a multicopper oxidoreductase (EC: 1.10.3.2), is a widely used enzyme in bioremediation of textile dye effluents. Fungal Laccase is preferably used as a remediating agent in the treatment and transformation of toxic organic pollutants. In this study, crude laccase from a basidiomycetes fungus, Phanerochaete sordida, was able to decolorize azo, antroquinone and indigoid dyes. In addition, interactions between dyes and enzyme were analysed using molecular docking studies.Methods:In this work, a white rot basidiomycete’s fungus, Phanerochaete sordida, was selected from forest soil isolates of Eastern Ghats, and Tirumala and lignolytic enzymes production was assayed after 7 days of incubation. The crude enzyme was checked for decolourisation of various synthetic textile dyes (Vat Brown, Acid Blue, Indigo, Reactive Blue and Reactive Black). Molecular docking studies were done using Autodock-4.2 to understand the interactions between dyes and enzymes.Results:Highest decolourisation efficiency was achieved with the crude enzyme in case of vat brown whereas the lowest decolourisation efficiency was achieved in Reactive blue decolourisation. Similar results were observed in their binding affinity with lignin peroxidase of Phanerochaete chrysosporium through molecular docking approach.Conclusion:Thus, experimental results and subsequent in silico validation involving an advanced remediation approach would be useful to reduce time and cost in other similar experiments.
APA, Harvard, Vancouver, ISO, and other styles
34

Czerwonka, Grzegorz, Iwona Konieczna, Paulina Żarnowiec, Artur Zieliński, Agnieszka Malinowska-Gniewosz, Agnieszka Gałuszka, Zdzisław Migaszewski, and Wiesław Kaca. "Characterization of Microbial Communities in Acidified, Sulfur Containing Soils." Polish Journal of Microbiology 66, no. 4 (December 4, 2017): 509–17. http://dx.doi.org/10.5604/01.3001.0010.7043.

Full text
Abstract:
Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil microbial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified by cultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established. Ureolytic activity, detected in soil extracts as well as in isolated Bacillus sp. strains may be considered as a tool for the bioremediation of acidified soils with high sulfate content.
APA, Harvard, Vancouver, ISO, and other styles
35

Pedroza-Rodríguez, Aura M., and Refugio Rodríguez-Vázquez. "Optimization of C/N Ratio and Inducers for Wastewater Paper Industry Treatment Using Trametes versicolor Immobilized in Bubble Column Reactor." Journal of Mycology 2013 (December 31, 2013): 1–11. http://dx.doi.org/10.1155/2013/536721.

Full text
Abstract:
C/N ratio and MnSO4 and CuSO4 concentrations were optimized for decolorization and chemical oxygen demand (COD) removal of bleached Kraft pulp mill effluent by Trametes versicolor immobilized in polyurethane foam. Statistical differences (P<0.0001) at high C/N ratios (169), 2 mM CuSO4, and 0.071 mM MnSO4 were determined. Decolorization of 60.5%, COD removal of 55%, laccase (LAC) 60 U/L, and manganese peroxidase (MnP) 8.4 U/L were obtained. Maximum of decolorization (82%), COD removal (83%), LAC (443.5 U/L), and MnP (18 U/L) activities at C/N ratio of 405 (6.75 mM CuSO4 and 0.22 mM MnSO4) was achieved in step 7 at 4 d. Positive correlation between the decolorization, COD removal, and enzymatic activity was found (P<0.0001). T. versicolor bioremediation capacity was evaluated in bubble column reactor during 8 d. Effluent was adjusted according to optimized parameters and treated at 25°C and air flow of 800 mL/min. Heterotrophic bacteria growth was not inhibited by fungus. After 4 d, 82% of COD reduction and 80% decolorization were recorded. Additionally, enzymatic activity of LAC (345 U/L) and MnP (78 U/L) was observed. The COD reduction and decolorization correlated positively (P<0.0001) with enzymatic activity. Chlorophenol removal was 98% of pentachlorophenol (PCP), 92% of 2,4,5-trichlorophenol (2,4,5-TCP), 90% of 3,4-dichlorophenol (3,4-DCP), and 99% of 4-chlorophenols (4CP).
APA, Harvard, Vancouver, ISO, and other styles
36

Avelino, Katielle Vieira, Marisangela Isabel Wietzikoski Halabura, Renan Alberto Marim, Nelma Lopes Araújo, Maria Graciela Iecher Faria Nunes, Dayane Lilian Gallani Silva, Giani Andrea Linde Colauto, Nelson Barros Colauto, and Juliana Silveira do Valle. "Coculture of white rot fungi enhance laccase activity and its dye decolorization capacity." Research, Society and Development 9, no. 11 (December 6, 2020): e88191110643. http://dx.doi.org/10.33448/rsd-v9i11.10643.

Full text
Abstract:
Fungal cocultures can promote complex interactions that result in physiological and biochemical alterations that favor the synergic and more efficient action of extracellular enzymes such as laccase. Thus, coculture can be used as a strategy to increase enzymatic activity, dye degradation, and bioremediation of textile effluents. This study aimed to evaluate the coculture effect of Lentinus crinitus, Pleurotus ostreatus, Pycnoporus sanguineus, and Trametes polyzona on laccase activity, mycelial biomass production, and in vitro decolorization of azo, anthraquinone, and triphenylmethane dyes. The species were cultivated in liquid medium in monoculture and coculture in paired combinations for 15 days to determine the laccase activity and produced mycelial biomass. The enzymatic extracts of fungal cultivations were used in decolorization tests of reactive blue 220 (RB220), malachite green (MG), and remazol brilliant blue R (RBBR). Pleurotus-Trametes, Lentinus-Pleurotus, and Lentinus-Trametes cocultures increase laccase activity compared to respective monocultures. Lentinus-Pycnoporus, Lentinus-Trametes, Lentinus-Pleurotus, and Pleurotus-Trametes cocultures stimulate mycelial biomass production in relation to their respective monocultures. The enzymatic extracts of monocultures and cocultures promoted the decolorization of all dyes. RB220 dye presented fast decolorization. In 24 h, all extracts reached maximum decolorization and the greatest color reduction percentage was 90% for Pleurotus-Trametes coculture extract. Pleurotus-Trametes extract also increased the decolorization of MG and RBBR dyes when compared to their respective monocultures in 48 h and 72 h, respectively. However, RBBR dye presented the greatest resistance to decolorization.
APA, Harvard, Vancouver, ISO, and other styles
37

Daccò, Chiara, Lidia Nicola, Marta Elisabetta Eleonora Temporiti, Barbara Mannucci, Federica Corana, Giovanna Carpani, and Solveig Tosi. "Trichoderma: Evaluation of Its Degrading Abilities for the Bioremediation of Hydrocarbon Complex Mixtures." Applied Sciences 10, no. 9 (April 30, 2020): 3152. http://dx.doi.org/10.3390/app10093152.

Full text
Abstract:
Hydrocarbons can have very harmful effects on organisms and the environment, and conventional techniques for their removal are expensive and require the use of chemicals and long-term actions. Trichoderma is an ascomycete genus known to be active on different recalcitrant substrates, since it can produce a set of nonspecific extracellular enzymes generally involved in the degradation of lignin. However, the literature concerning the use of Trichoderma to degrade hydrocarbons is still limited. In this work we aimed to investigate the ability of Trichoderma to exploit used engine oil as its sole carbon source for prospective bioremediation of contaminated substrates. Four different strains belonging to Trichoderma asperellum and Trichoderma harzianum species were tested. The fungi were inoculated in direct contact with used engine oil, and after 45 days the samples were analyzed by gas chromatography/mass spectrometry (GC/MS). The results showed that all strains (except Trichoderma asperellum F1020) significantly changed the oil composition, decreasing the aromatic fraction in favor of the aliphatic one. T. harzianum F26, especially, showed a significant reduction of the BTEX (benzene, toluene, ethylbenzene, and the three xylene isomers) and alkylbenzenes fraction and an increase in short-chain aliphatics C1–C20. Enzymatic tests for laccase and peroxidase were also carried out, demonstrating that every strain seems to express a different mode of action.
APA, Harvard, Vancouver, ISO, and other styles
38

Dzionek, Anna, Jolanta Dzik, Danuta Wojcieszyńska, and Urszula Guzik. "Fluorescein Diacetate Hydrolysis Using the Whole Biofilm as a Sensitive Tool to Evaluate the Physiological State of Immobilized Bacterial Cells." Catalysts 8, no. 10 (October 2, 2018): 434. http://dx.doi.org/10.3390/catal8100434.

Full text
Abstract:
Due to the increasing interest and the use of immobilized biocatalysts in bioremediation studies, there is a need for the development of an assay for quick and reliable measurements of their overall enzymatic activity. Fluorescein diacetate (FDA) hydrolysis is a widely used assay for measuring total enzymatic activity (TEA) in various environmental samples or in monoculture researches. However, standard FDA assays for TEA measurements in immobilized samples include performing an assay on cells detached from the carrier. This causes an error, because it is not possible to release all cells from the carrier without affecting their metabolic activity. In this study, we developed and optimized a procedure for TEA quantification in the whole biofilm formed on the carrier without disturbing it. The optimized method involves pre-incubation of immobilized carrier in phosphate buffer (pH 7.6) on the orbital shaker for 15 min, slow injection of FDA directly into the middle of the immobilized carrier, and incubation on the orbital shaker (130 rpm, 30 °C) for 1 h. Biofilm dry mass was obtained by comparing the dried weight of the immobilized carrier with that of the unimmobilized carrier. The improved protocol provides a simple, quick, and more reliable quantification of TEA during the development of immobilized biocatalysts compared to the original method.
APA, Harvard, Vancouver, ISO, and other styles
39

Handrianto, Prasetyo. "MIKROORGANISME PENDEGRADASI TPH (TOTAL PETROLEUM HYDROCARBON) SEBAGAI AGEN BIOREMEDIASI TANAH TERCEMAR MINYAK BUMI (Review Article)." Jurnal SainHealth 2, no. 2 (November 27, 2018): 35. http://dx.doi.org/10.51804/jsh.v2i2.287.35-42.

Full text
Abstract:
Exploitation and exploration activities will produce sewage sludge and crude oil spills that cause pollution to the environment and upgrading to the environment, biology and soil chemistry. Monitoring of oil pollution conditions on the soil can be done by detection of all hydrocarbon components, or what is called the total petroleum hydrocarbon (TPH). According to its components, this total petroleum hydrocarbon (TPH) can be classified into 3 points, aliphatic, alicyclic, and aromatic. One of the biological efforts that can be used to overcome petroleum pollution is by using bioremediation technology. There are several methods in bioremediation, one of which is the biostimulation method, where the growth of the original hydrocarbon decomposers is stimulated by adding nutrients, oxygen, pH optimization and temperature. Hydrocarbonoclastic microorganisms have characteristic not possessed by other microorganisms, namely their ability to excrete hydroxylase enzymes, which are hydrocarbon oxidizing enzymes, so that these bacteria can degrade petroleum hydrocarbons. Biodegradation can be formed if there is a structural transformation so that cahnges in molecular integrity occur. This process is a series of enzymatic or biochemical reaction that require ideal environmental conditions with the growth and proliferation of microorganisms. Something that need to be known before remediation are pollutants (organic or inorganic), degraded/ not, dangerous/ not, how many pollutants pollute the soil, the ratio of carbon (C), Nitrogen (N), and phophorus (P), soil type, soil conditions (wet dry), and how long pollutants have been deposited in these locations
APA, Harvard, Vancouver, ISO, and other styles
40

Tortella, G. R., O. Rubilar, L. Gianfreda, E. Valenzuela, and M. C. Diez. "Enzymatic characterization of Chilean native wood-rotting fungi for potential use in the bioremediation of polluted environments with chlorophenols." World Journal of Microbiology and Biotechnology 24, no. 12 (July 19, 2008): 2805–18. http://dx.doi.org/10.1007/s11274-008-9810-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
41

Akhter, Mursheda, Lolo Wal Marzan, Yasmin Akter, and Kazuyuki Shimizu. "Microbial Bioremediation of Feather Waste for Keratinase Production: An Outstanding Solution for Leather Dehairing in Tanneries." Microbiology Insights 13 (January 2020): 117863612091328. http://dx.doi.org/10.1177/1178636120913280.

Full text
Abstract:
In leather industries and tanneries, large amount of wastes has been disposed; which polluting water, soil, and atmosphere and causing serious human health problems. In particular, chemical dehairing process of leather industries produces fair amount of toxic wastes. It is, thus, urgently needed to use alternative processes free from pollution. As more than 90% of keratin is contained in feather, it is desirable to develop bioremediation process using keratinolytic microorganisms. In the present investigation, therefore, we first identified Bacillus cereus and Pseudomonas sp. to be able to produce keratinase. Then, the optimization was performed to maximize the keratinase activity with respect to cultivation temperature, pH, and incubation time. Moreover, the effects of metal ions and various substrates on keratinase activity were also investigated. The result indicates that keratinase activity became maximum at 50°C for both strains, whereas the optimal pH was 10.0 for B. cereus and 7.0 for Pseudomonas sp. The highest keratinase activity of 74.66 ± 1.52 U/mL was attained by B. cereus, whereas 57.66 ± 2.52 U/mL was attained by Pseudomonas sp. Enzymatic dehairing efficiency of leathers was also compared with chemical dehairing (Na2S and CaO), where complete dehairing was achieved by treating them with crude keratinase. Partial enzyme purification was performed by acetone precipitation. Batch cultivation of B. cereus using 1 L fermentor indicates a potential candidate for large-scale keratinase production. Thus, keratinase enzyme by degrading poultry wastes (feather) can be an alternative approach to chemical dehairing in leather industries, thus preventing environmental pollution through bioremediation.
APA, Harvard, Vancouver, ISO, and other styles
42

Vimalnath, S., H. Ravishankar, C. Schwandt, R. V. Kumar, and S. Subramanian. "Mechanistic studies on the biosorption of Pb(II) by Pseudomonas aeruginosa." Water Science and Technology 78, no. 2 (July 3, 2018): 290–300. http://dx.doi.org/10.2166/wst.2018.296.

Full text
Abstract:
Abstract The biosorption of Pb(II) ions from aqueous solution has been studied using both the intact and thermolyzed cells of Pseudomonas aeruginosa. Further, the role of the major cell wall components, namely DNA, protein, polysaccharide, and lipid, in Pb(II) binding has been assessed using an enzymatic treatment method. The Pb(II) bioremediation capability of P. aeruginosa cells has been investigated by varying the parameters of pH, time of interaction, amount of biomass, and concentration of Pb(II). The complete bioremoval of Pb(II) using intact cells has been achieved for an initial Pb(II) concentration of 12.4 mg L−1 at pH 6.2 and temperature 29 ± 1 °C. The biosorption isotherm follows Langmuirian behavior with a Gibbs free energy of −30.7 kJ mol−1, indicative of chemisorption. The biosorption kinetics is consistent with a pseudo-second-order model. The possible Pb(II) binding mechanisms of P. aeruginosa cells are discussed based on characterization using zeta potential measurements, Fourier transform infra-red spectroscopy, and energy dispersive X-ray spectroscopy. The results confirm that among the major cell wall components studied, polysaccharide shows the highest contribution towards Pb(II) binding, followed by DNA, lipid, and protein. Similar studies using thermolyzed cells show higher Pb(II) uptake compared to the intact cells both before and after enzymatic treatment.
APA, Harvard, Vancouver, ISO, and other styles
43

Perini, Brayam Luiz Batista, Rodrigo Luiz Bitencourt, Naionara Ariete Daronch, Andréa Lima dos Santos Schneider, and Débora de Oliveira. "Surfactant-enhanced in-situ enzymatic oxidation: A bioremediation strategy for oxidation of polycyclic aromatic hydrocarbons in contaminated soils and aquifers." Journal of Environmental Chemical Engineering 8, no. 4 (August 2020): 104013. http://dx.doi.org/10.1016/j.jece.2020.104013.

Full text
APA, Harvard, Vancouver, ISO, and other styles
44

Shushkova, T. V., D. O. Epiktetov, S. V. Tarlachkov, I. T. Ermakova, and A. A. Leontievskii. "Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems." Biotekhnologiya 36, no. 4 (2020): 126–35. http://dx.doi.org/10.21519/0234-2758-2020-36-4-126-135.

Full text
Abstract:
The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.
APA, Harvard, Vancouver, ISO, and other styles
45

Moreira Neto, Sérgio Luiz, Dácio Roberto Matheus, and Kátia Maria Gomes Machado. "Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes." Brazilian Archives of Biology and Technology 52, no. 5 (October 2009): 1075–82. http://dx.doi.org/10.1590/s1516-89132009000500003.

Full text
Abstract:
The basidiomycete fungi Lentinus crinitus and Psilocybe castanella are being evaluated in a bioremediation process of soils contaminated with organochlorine industrial residues in the Baixada Santista, São Paulo. The aim of the present study was to determine the influence of pH on the fungal growth, in vitro decolorization of anthraquinonic dye Remazol Brilliant Blue R (RBBR) and laccase activity. The pH of the culture medium influenced the growth of L. crinitus and P. castanella, which presented less growth at pH 5.9 and pH 2.7, respectively. The fungi were able to modify the pH of the culture medium, adjusting it to the optimum pH for growth which was close to 4.5. Decolorization of the RBBR was maximal at a pH of 2.5 to 3.5. Higher laccase activity was observed at pH 3.5 and pH 4.5 for L. crinitus and P. castanella, respectively. pH was found to be an important parameter for both the growth of these fungi and the enzymatic system involved in RBBR decolorization.
APA, Harvard, Vancouver, ISO, and other styles
46

Padilla-Crespo, Elizabeth, Jun Yan, Cynthia Swift, Darlene D. Wagner, Karuna Chourey, Robert L. Hettich, Kirsti M. Ritalahti, and Frank E. Löffler. "Identification and Environmental Distribution ofdcpA, Which Encodes the Reductive Dehalogenase Catalyzing the Dichloroelimination of 1,2-Dichloropropane to Propene in Organohalide-Respiring Chloroflexi." Applied and Environmental Microbiology 80, no. 3 (November 15, 2013): 808–18. http://dx.doi.org/10.1128/aem.02927-13.

Full text
Abstract:
ABSTRACTDehalococcoides mccartyistrains KS and RC grow with 1,2-dichloropropane (1,2-D) as an electron acceptor in enrichment cultures derived from hydrocarbon-contaminated and pristine river sediments, respectively. Transcription, expression, enzymatic, and PCR analyses implicated the reductive dehalogenase genedcpAin 1,2-D dichloroelimination to propene and inorganic chloride. Quantitative real-time PCR (qPCR) analyses demonstrated aD. mccartyicell increase during growth with 1,2-D and suggested that bothD. mccartyistrains carried a singledcpAgene copy per genome.D. mccartyistrain RC and strain KS produced 1.8 × 107± 0.1 × 107and 1.4 × 107± 0.5 × 107cells per μmol of propene formed, respectively. ThedcpAgene was identified in 1,2-D-to-propene-dechlorinating microcosms established with sediment samples collected from different geographical locations in Europe and North and South America. Clone library analysis revealed two distinctdcpAphylogenetic clusters, both of which were captured by thedcpAgene-targeted qPCR assay, suggesting that the qPCR assay is useful for site assessment and bioremediation monitoring at 1,2-D-contaminated sites.
APA, Harvard, Vancouver, ISO, and other styles
47

Enguita, Francisco J., and Ana Lúcia Leitão. "Hydroquinone: Environmental Pollution, Toxicity, and Microbial Answers." BioMed Research International 2013 (2013): 1–14. http://dx.doi.org/10.1155/2013/542168.

Full text
Abstract:
Hydroquinone is a major benzene metabolite, which is a well-known haematotoxic and carcinogenic agent associated with malignancy in occupational environments. Human exposure to hydroquinone can occur by dietary, occupational, and environmental sources. In the environment, hydroquinone showed increased toxicity for aquatic organisms, being less harmful for bacteria and fungi. Recent pieces of evidence showed that hydroquinone is able to enhance carcinogenic risk by generating DNA damage and also to compromise the general immune responses which may contribute to the impaired triggering of the host immune reaction. Hydroquinone bioremediation from natural and contaminated sources can be achieved by the use of a diverse group of microorganisms, ranging from bacteria to fungi, which harbor very complex enzymatic systems able to metabolize hydroquinone either under aerobic or anaerobic conditions. Due to the recent research development on hydroquinone, this review underscores not only the mechanisms of hydroquinone biotransformation and the role of microorganisms and their enzymes in this process, but also its toxicity.
APA, Harvard, Vancouver, ISO, and other styles
48

Korniłłowicz-Kowalska, Teresa, and Kamila Rybczyńska-Tkaczyk. "Growth conditions, physiological properties, and selection of optimal parameters of biodegradation of anticancer drug daunomycin in industrial effluents by Bjerkandera adusta CCBAS930." International Microbiology 23, no. 2 (November 12, 2019): 287–301. http://dx.doi.org/10.1007/s10123-019-00102-3.

Full text
Abstract:
Abstract The study characterizes the anamorphic Bjerkandera adusta strain CCBAS 930, including growth conditions, physiological properties, and enzymatic activities related to basic metabolism and specific properties coupled with the fungal secondary metabolism. It was established that the fungus grows in a wide pH range (3.5–7.5), up to 3% of salt concentration and a temperature of 5–30 °C. Media rich in natural organic components (potato, maize extracts, whey) are optimal for biomass propagation. Minimal media, containing mineral salts and glucose as well as static growth conditions, are required to obtain idiophasic mycelium, equivalent to the secondary metabolism of the fungus. Of the 7 complex C, N, and energy sources tested, the strain did not utilize only fibrous cellulose. Lipolytic activity reached the highest values of the enzymatic activities corresponding to those capabilities. The specific properties of strain B. adusta CCBAS 930 determined by the production of HRP-like peroxidase were related to the decolorization and biodegradation of anthraquinone derivative daunomycin. The decolorization of 30% of daunomycin effluents occurred most rapidly in iso-osmotic medium and non-enriched with nitrogen, containing 0.25% glucose, pH = 5.0–6.0, and 25–30 °C. In agitated cultures, the strain decolorized solutions of daunomycin by biosorption, which coincided with the inhibition of aerial mycelium production and HRP-like biosynthesis. Based on knowledge, potential and real possibilities of using the strain in bioremediation of colored industrial sewage were discussed.
APA, Harvard, Vancouver, ISO, and other styles
49

Esterhuizen-Londt, Maranda, Katrin Schwartz, and Stephan Pflugmacher. "Using aquatic fungi for pharmaceutical bioremediation: Uptake of acetaminophen by Mucor hiemalis does not result in an enzymatic oxidative stress response." Fungal Biology 120, no. 10 (October 2016): 1249–57. http://dx.doi.org/10.1016/j.funbio.2016.07.009.

Full text
APA, Harvard, Vancouver, ISO, and other styles
50

Adnan, Liyana Amalina, Palanivel Sathishkumar, Abdull Rahim Mohd Yusoff, Tony Hadibarata, and Fuad Ameen. "Rapid bioremediation of Alizarin Red S and Quinizarine Green SS dyes using Trichoderma lixii F21 mediated by biosorption and enzymatic processes." Bioprocess and Biosystems Engineering 40, no. 1 (September 23, 2016): 85–97. http://dx.doi.org/10.1007/s00449-016-1677-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography