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Journal articles on the topic 'Enzymatic cleaning'

Author: Grafiati

Published: 7 September 2024

Last updated: 31 July 2025

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1

Bdiri, Bensghaier, Chaabane, Kozmai, Baklouti, and Larchet. "Preliminary Study on Enzymatic-Based Cleaning of Cation-Exchange Membranes Used in Electrodialysis System in Red Wine Production." Membranes 9, no. 9 (2019): 114. http://dx.doi.org/10.3390/membranes9090114.

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The use of enzymatic agents as biological solutions for cleaning ion-exchange membranes fouled by organic compounds during electrodialysis (ED) treatments in the food industry could be an interesting alternative to chemical cleanings implemented at an industrial scale. This paper is focused on testing the cleaning efficiency of three enzyme classes (β-glucanase, protease, and polyphenol oxidase) chosen for their specific actions on polysaccharides, proteins, and phenolic compounds, respectively, fouled on a homogeneous cation-exchange membrane (referred CMX-Sb) used for tartaric stabilization

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2

Graßhoff, A. "Enzymatic Cleaning of Milk Pasteurizers." Food and Bioproducts Processing 80, no. 4 (2002): 247–52. http://dx.doi.org/10.1205/096030802321154736.

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3

Gonzalez, Jonathan, Thomas Vanzieleghem, Axelle Dumazy, et al. "On-site comparison of an enzymatic detergent and a non-enzymatic detergent-disinfectant for routine manual cleaning of flexible endoscopes." Endoscopy International Open 07, no. 04 (2019): E412—E420. http://dx.doi.org/10.1055/a-0838-4995.

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Abstract Background and study aims Flexible endoscopes are potential vectors of pathogen transmission to patients that are subjected to cleaning and high-level disinfection after each procedure. Efficient manual cleaning is a prerequisite for effective high-level disinfection. The goal of this study was to demonstrate the impact of the cleaning chemistry in the outcome of the manual cleaning of endoscopes. Materials and methods Twelve endoscopes were included in this study: four colonoscopes, four gastroscopes, two duodenoscopes and two bronchoscopes. This study was designed with two phases; i

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Persson, Mette, K. Bilgrav, Lone Jensen, and F. Gottrup. "Enzymatic Wound Cleaning and Absorbable Sutures." European Surgical Research 18, no. 2 (1986): 122–28. http://dx.doi.org/10.1159/000128514.

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Bárbara Guandalini, Ivana Vendramini, Denise Piotto Leonardi, Flávia Sens Fagundes Tomazinho1, and Paulo Henrique Tomazinho. "Comparative analysis of four cleaning methods of endodontic files." RSBO 11, no. 2 (2015): 154–8. http://dx.doi.org/10.21726/rsbo.v11i2.837.

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Due to the size and design of endodontic files, these instruments have been considered one of the most difficult to clean among all dental instruments. The debris maintenance within the sulcus prevents the effective sterilization and may compromise the disinfection of root canal systems in endodontic therapy. However, there is neither a method nor technique that standardized the cleaning of these instruments. Objective: To evaluate the cleaning ability of four techniques used in dentistry. Material and methods: For this purpose, 30 new size #40 Flexofile were used for the preparation of the ca

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Chernobai, V. T., P. I. Kabachnyi, V. F. Rudyuk, et al. "Asperase ? An enzymatic preparation for cleaning wounds." Pharmaceutical Chemistry Journal 25, no. 1 (1991): 60–61. http://dx.doi.org/10.1007/bf00766368.

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7

Walker, Natalie, FJ Trevor Burke, and Charles J. Palenik. "Comparison of Ultrasonic Cleaning Schemes: A Pilot Study." Primary Dental Care os13, no. 2 (2006): 51–56. http://dx.doi.org/10.1308/135576106776337904.

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Objective Ultrasonic cleaning is an effective method for cleaning dental instruments prior to sterilisation. However, there are few studies that directly compare precleaning and ultrasonic cleaning solutions. This study evaluated the efficacy of different ultrasonic cleaning schemes. Method and Materials Twenty representative dental instruments, five of which were soiled with a mixture of blood and hydroxyapatite, were used in a series of cleaning runs. Cleaning employed a presoaking agent, ultrasonic cleaning, or a combination of both. Two presoaking agents (Non-ionic Ultrasonic Cleaning Solu

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Rudolph, Gregor, Herje Schagerlöf, Kristian Morkeberg Krogh, Ann-Sofi Jönsson, and Frank Lipnizki. "Investigations of Alkaline and Enzymatic Membrane Cleaning of Ultrafiltration Membranes Fouled by Thermomechanical Pulping Process Water." Membranes 8, no. 4 (2018): 91. http://dx.doi.org/10.3390/membranes8040091.

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The pulp and paper industry is one of the most important industrial sectors worldwide, and has considerable potential for the sustainable fractionation of lignocellulosic biomass to provide valuable compounds. Ultrafiltration (UF) is a suitable separation technique for the profitable production of hemicelluloses from process water from thermomechanical pulping (ThMP), but is limited by membrane fouling. Improvements in cleaning protocols and new alternative cleaning agents are required to ensure a long membrane lifetime, and thus a sustainable process. This study, therefore, focuses on the cle

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Allie, Z., E. P. Jacobs, A. Maartens, and P. Swart. "Enzymatic cleaning of ultrafiltration membranes fouled by abattoir effluent." Journal of Membrane Science 218, no. 1-2 (2003): 107–16. http://dx.doi.org/10.1016/s0376-7388(03)00145-5.

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10

te Poele, Sandy, and Jaap van der Graaf. "Enzymatic cleaning in ultrafiltration of wastewater treatment plant effluent." Desalination 179, no. 1-3 (2005): 73–81. http://dx.doi.org/10.1016/j.desal.2004.11.056.

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11

Lawson, Victoria A., James D. Stewart, and Colin L. Masters. "Enzymatic detergent treatment protocol that reduces protease-resistant prion protein load and infectivity from surgical-steel monofilaments contaminated with a human-derived prion strain." Journal of General Virology 88, no. 10 (2007): 2905–14. http://dx.doi.org/10.1099/vir.0.82961-0.

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The unconventional nature of the infectious agent of prion diseases poses a challenge to conventional infection control methodologies. The extraneural tissue distribution of variant and sporadic Creutzfeldt–Jakob disease has increased concern regarding the risk of prion disease transmission via general surgical procedures and highlighted the need for decontamination procedures that can be incorporated into routine processing. In this study, the ability of preparations of enzymatic medical instrument cleaners to reduce the infectivity associated with a rodent-adapted strain of human prion disea

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12

Fagerlund, Annette, Even Heir, Trond Møretrø, and Solveig Langsrud. "Listeria Monocytogenes Biofilm Removal Using Different Commercial Cleaning Agents." Molecules 25, no. 4 (2020): 792. http://dx.doi.org/10.3390/molecules25040792.

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Effective cleaning and disinfection (C&D) is pivotal for the control of Listeria monocytogenes in food processing environments. Bacteria in biofilms are protected from biocidal action, and effective strategies for the prevention and removal of biofilms are needed. In this study, different C&D biofilm control strategies on pre-formed L. monocytogenes biofilms on a conveyor belt material were evaluated and compared to the effect of a conventional chlorinated, alkaline cleaner (agent A). Bacterial reductions up to 1.8 log were obtained in biofilms exposed to daily C&D cycles with norm

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Vanangamudi, Anbharasi, Ludovic Dumée, Mikel Duke, and Xing Yang. "Dual Functional Ultrafiltration Membranes with Enzymatic Digestion and Thermo-Responsivity for Protein Self-Cleaning." Membranes 8, no. 3 (2018): 85. http://dx.doi.org/10.3390/membranes8030085.

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Controlling surface–protein interaction during wastewater treatment is the key motivation for developing functionally modified membranes. A new biocatalytic thermo-responsive poly vinylidene fluoride (PVDF)/nylon-6,6/poly(N-isopropylacrylamide)(PNIPAAm) ultrafiltration membrane was fabricated to achieve dual functionality of protein-digestion and thermo-responsive self-cleaning. The PVDF/nylon-6,6/PNIPAAm composite membranes were constructed by integrating a hydrophobic PVDF cast layer and hydrophilic nylon-6,6/PNIPAAm nanofiber layer on to which trypsin was covalently immobilized. The enzyme

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Bilad, M. R., M. Baten, A. Pollet, et al. "A novel In-situ Enzymatic Cleaning Method for Reducing Membrane Fouling in Membrane Bioreactors (MBRs)." Indonesian Journal of Science and Technology 1, no. 1 (2016): 1. http://dx.doi.org/10.17509/ijost.v1i1.2211.

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A novel in-situ enzymatic cleaning method was developed for fouling control in membrane bioreactors (MBRs). It is achieved by bringing the required enzymes near the membrane surface by pulling the enzymes to a magnetic membrane (MM) surface by means of magnetic forces, exactly where the cleaning is required. To achieve this, the enzyme was coupled to a magnetic nanoparticle (MNP) and the membrane it self was loaded with MNP. The magnetic activity was turned by means of an external permanent magnet. The effectiveness of concept was tested in a submerged membrane filtration using the model enzym

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Argüello, Maria A., Silvia Álvarez, Francisco A. Riera, and Ricardo Álvarez. "Enzymatic Cleaning of Inorganic Ultrafiltration Membranes Fouled by Whey Proteins." Journal of Agricultural and Food Chemistry 50, no. 7 (2002): 1951–58. http://dx.doi.org/10.1021/jf0107510.

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16

PETRUS, H., H. LI, V. CHEN, and N. NORAZMAN. "Enzymatic cleaning of ultrafiltration membranes fouled by protein mixture solutions." Journal of Membrane Science 325, no. 2 (2008): 783–92. http://dx.doi.org/10.1016/j.memsci.2008.09.004.

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17

Stiefel, Philipp, Stefan Mauerhofer, Jana Schneider, Katharina Maniura-Weber, Urs Rosenberg, and Qun Ren. "Enzymes Enhance Biofilm Removal Efficiency of Cleaners." Antimicrobial Agents and Chemotherapy 60, no. 6 (2016): 3647–52. http://dx.doi.org/10.1128/aac.00400-16.

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Efficient removal of biofilms from medical devices is a big challenge in health care to avoid hospital-acquired infections, especially from delicate devices like flexible endoscopes, which cannot be reprocessed using harsh chemicals or high temperatures. Therefore, milder solutions such as enzymatic cleaners have to be used, which need to be carefully developed to ensure efficacious performance.In vitrobiofilm in a 96-well-plate system was used to select and optimize the formulation of novel enzymatic cleaners. Removal of the biofilm was quantified by crystal violet staining, while the disinfe

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Lucimara Albrecht, Erica Lopes Ferreira, Maria Luiza Minuzzi Passos, and Rossana Tais Cecchetti. "Teeth processing in human teeth bank – proposal of protocol." RSBO 10, no. 4 (2014): 386–93. http://dx.doi.org/10.21726/rsbo.v10i4.955.

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Dentistry courses conduct preclinical laboratory trainings and research using extracted human teeth. For a safe usage of those teeth, it is necessary to subject them to cleaning, disinfection and/or sterilization and proper storage to ensure they are free of biological residues. Each of these steps should be properly described in standard operating procedures (SOPs) and in the processing protocol of the Human Tooth Bank (HTB). Objective:To create a processing protocol for Human Tooth Bank based on a literature review on cleaning, disinfection and/or sterilization and storage methods for extrac

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19

Mishra, A. A., R. N. Shukla, and L. K. Rawat. "Enzymatic Wet milling and Dry milling Process of Corn." European Journal of Food Science and Technology 12, no. 2 (2024): 62–69. https://doi.org/10.37745/ejfst.2013/vol12n26269.

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Enzymatic corn wet milling (E-Milling) is a method derived from conventional wet milling for the recuperation and purification of starch and co-merchandise. The usage of enzymes in wet milling process eliminates the needs of SO2 and also reduces the steeping time. The E-Milling method consists of grain cleaning, pretreatment, enzymatic treatment, germ separation and recovery, fiber separation and restoration, gluten separation and restoration and starch separation. The E-Milling process turned into discovered the price aggressive with the conventional method for the duration of excessive corn

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20

Lopes, Cristiane de Lion Botero Couto, Kazuko Uchikawa Graziano, and Terezinha de Jesus Andreoli Pinto. "Evaluation of single-use reprocessed laparoscopic instrument sterilization." Revista Latino-Americana de Enfermagem 19, no. 2 (2011): 370–77. http://dx.doi.org/10.1590/s0104-11692011000200020.

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This experimental, comparative, laboratory study evaluated the effectiveness of the sterilization of single-use laparoscopic instruments - SULIs (grasper, dissector, scissors, Veress needle and electrosurgical probe system), after contamination-challenge with bacterial spores and sheep blood, and compared the results of the sterilization tests with those of the equivalent reusable instruments. The cleaning methods used were; ultrasonic washer with pulsatile water jet and enzymatic detergent, manual cleaning, cleaning with pressurized water and rinsing. The SULIs were sterilized with ethylene o

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21

Penna, Thereza Christina Vessoni, and Carlos Augusto M. Ferraz. "Cleaning of Blood-Contaminated Reprocessed Angiographic Catheters and Spinal Needles." Infection Control & Hospital Epidemiology 21, no. 8 (2000): 499–504. http://dx.doi.org/10.1086/501793.

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Objectives:To evaluate the efficacy of a multistep cleaning method using a cleaner and a chemical disinfectant on blood-contaminated angiographic catheters and spinal needles intended to be sterilized by hydrogen peroxide gas plasma.Method:A mixture of radiopaque iodine contrast, bovine blood (plus ethylenediaminetetraacetic acid), and a suspension of Bacillus subtilis spores was used to simulate catheterization and needle use. The mixture was a 1:1 proportion of contrast and blood, inoculated so that there was a final concentration of B subtilis spores of 1.0×106 colony-forming units (CFU)/mL

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22

Whitworth, Christine L., Karen Davies, and Nikolaus OA Palmer. "Can Protein Contamination be Removed from Hand Endodontic Instruments?" Primary Dental Care os16, no. 1 (2009): 7–12. http://dx.doi.org/10.1308/135576109786994569.

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Aim The aim of this study was to quantify total protein adhering to hand endodontic files and to measure and compare the efficacy of ultrasonic cleaning and washer-disinfectors, with and without presoaking, in protein removal from clinically contaminated endodontic files. Method Total protein contamination of the endodontic files was quantified using an assay reagent colorimetric method. Twelve general dental practitioners were recruited to collect clinically contaminated files. One hundred and fifty clinically contaminated files were allowed to air-dry in sterile plastic containers and a furt

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23

Phalalo, Betty Lame, James Hungo Kimotho, and Naomi Maina. "An enzymatic based formulation for cleaning and disinfection of medical devices." Advanced Studies in Biology 13, no. 1 (2021): 45–59. http://dx.doi.org/10.12988/asb.2021.91297.

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24

Argüello, M. "Enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation." Journal of Membrane Science 216, no. 1-2 (2003): 121–34. http://dx.doi.org/10.1016/s0376-7388(03)00064-4.

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25

Onaizi, Sagheer A., Lizhong He, and Anton P. J. Middelberg. "The construction, fouling and enzymatic cleaning of a textile dye surface." Journal of Colloid and Interface Science 351, no. 1 (2010): 203–9. http://dx.doi.org/10.1016/j.jcis.2010.07.030.

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26

Costa, Dayane, Roel Castillo, Lillian Kelly Lopes, Anaclara Tipple, Honghua Hu, and Karen Vickery. "Efficacy of Double Manual Cleaning Versus Automated Cleaning for Removal of Biofilm of Hinged Surgical Instruments." Infection Control & Hospital Epidemiology 41, S1 (2020): s518—s519. http://dx.doi.org/10.1017/ice.2020.1200.

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Objectives: To evaluate the efficacy of double manual cleaning (DMC) with enzymatic followed by alkaline detergent for removing biofilm on hinged surgical instruments compared to automated cleaning by the washer-disinfector. Methods: Biofilm of Staphylococcus aureus (ATCC 25923) was formed in vitro on hemostatic forceps (Fig. 1). Biofilm-covered forceps were rinsed in distilled water and subjected to one of the following cleaning regimes (n = 5 forceps each): Group 1 forceps were soaked in sterile water for 5 minutes. Group 2-DMC forceps were soaked in enzymatic detergent, brushed 5 times on e

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Wang, Yi-Fan, Yu Wu, Xiao-Wei Liu, et al. "Effect of a disposable endoscope precleaning kit in the cleaning procedure of gastrointestinal endoscope: A multi-center observational study." World Journal of Gastrointestinal Endoscopy 15, no. 12 (2023): 705–14. http://dx.doi.org/10.4253/wjge.v15.i12.705.

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BACKGROUND Precleaning is a key step in endoscopic reprocessing. AIM To develop an effective and economic endoscope cleaning method by using a disposable endoscope bedside precleaning kit. METHODS Altogether, 228 used gastrointestinal endoscopes were selected from five high-volume endoscopy units and precleaned by a traditional precleaning bucket (group T) or a disposable endoscope bedside precleaning kit (group D). Each group was further subdivided based on the replacement frequency of the cleaning solution, which was replaced every time in subgroups T1 and D1 and every several times in subgr

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Khan, Mohiuddin Md Taimur, William Mickols, Steffen Danielsen, Keith Thomsen, and Anne Camper. "Cryosectioning diagnosis and enzymatic cleaning of reverse osmosis membrane biofouling: Part I." Membrane Technology 2020, no. 8 (2020): 5–10. http://dx.doi.org/10.1016/s0958-2118(20)30142-7.

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Khan, Mohiuddin Md Taimur, William Mickols, Steffen Danielsen, Keith Thomsen, and Anne Camper. "Cryosectioning diagnosis and enzymatic cleaning of reverse osmosis membrane biofouling: Part II." Membrane Technology 2020, no. 9 (2020): 5–8. http://dx.doi.org/10.1016/s0958-2118(20)30158-0.

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Tsiaprazi-Stamou, Artemis, Irene Ylla Monfort, Anna M. Romani, Serafim Bakalis, and Konstantinos Gkatzionis. "The synergistic effect of enzymatic detergents on biofilm cleaning from different surfaces." Biofouling 35, no. 8 (2019): 883–99. http://dx.doi.org/10.1080/08927014.2019.1666108.

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31

Portes Canongia, Ana Carolina, Daniela Sales Alviano Moreno, Leida Gomes Abraçado, Matheus Melo Pithon, and Mônica Tirre Araújo. "Effectiveness of methods for cleaning arch wire: an in vitro study." Bioscience Journal 37 (February 25, 2021): e37017. http://dx.doi.org/10.14393/bj-v37n0a2021-55339.

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The aim of this study was to evaluate various methods of removing bacterial and fungus biofilm, to simulate orthodontic arch wires cleaning before reinsertion in the patients appliance. Rectangular Nickel Titanium (NiTi), Stainless Steel (SS) and Titanium Molybdenum (TMA) wires were divided into five groups, then contaminated with strains of Streptococcus mutans and Candida albicas. Four segments of each group served as control and were not contaminated. Six cleanings methods were used to remove the biofilm: cotton roll and a chemical agent (chlorhexidine, sodium hypochlorite, 70% alcohol), co

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Kim, Beomsoo, Young-Sin Choi, Jun-Koo Kang, et al. "Outbreak of Cystoscopy-Related Urinary Tract Infections With Pseudomonas aeruginosa in South Korea, 2022: A Case Series." Urogenital Tract Infection 19, no. 3 (2024): 97–103. https://doi.org/10.14777/uti.2448028014.

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Purpose: This study conducted an epidemiological investigation of <i>Pseudomonas aeruginosa</i> urinary tract infections (UTIs) following cystoscopy at Chilgok Kyungpook National University Hospital.Materials and Methods: From May 16 to July 15, 2022, among 353 patients who underwent cystoscopy, 6 patients reported febrile UTIs following cystoscopy. They were admitted to the urology department of the hospital after visiting the Emergency Department. <i>P. aeruginosa</i> was found in the urine cultures of 4 of the 6 hospitalized patients. During the epidemiological inves

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Bryndina, L. V., O. V. Baklanova, and N. M. Il’ina. "Biopreparats for Soil Cleaning from Pollution Based on Organic Waste." Ecology and Industry of Russia 23, no. 10 (2019): 20–23. http://dx.doi.org/10.18412/1816-0395-2019-10-20-23.

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Studies have been carried out to obtain combined biopreparats obtained on the basis of sewage sludge (WWS) and activated carbon (AC) from plant materials for cleaning soils from contaminants with herbicides. The content of organic matter in the settled sludge is 57.3 %. The organic matter of sewage sludge activates its enzymatic activity. Catalase activity in samples treated with combined sorbents, 2.5 to 2.9 times higher than in control soil samples. The combined use of WWS and activated carbons from plant residues significantly accelerates the decomposition of the herbicide. The presence of

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ALVAREZ PENEDO, Berta, Sandra FORSTNER, and Alexandru RUSU. "ENTHALPY EU PROJECT: ENABLING THE DRYING PROCESS TO SAVE ENERGY AND WATER, REALISING PROCESS EFFICIENCY IN THE DAIRY CHAIN." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology 73, no. 2 (2016): 157. http://dx.doi.org/10.15835/buasvmcn-fst:12299.

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The EU funded ENTHALPY project aims to significantly reduce the consumption of water and energy in milk powder production to increase efficiency in the dairy production chain. Using a systematic approach, ENTHALPY project focusses on innovations within the post-harvest chain representing the highest energy and water consumption such as RF heating, solar thermal energy, mono-disperse atomising, dryer modelling, inline monitoring, enzymatic cleaning and membrane technology,

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Arpan Choudhary, Manas Sharma, Deep Kumar Jain, and Vishal Kirti Jain. "An insight into outbreak of atypical mycobacterial infection following percutaneous nephrolithotomy - Role of cleaning and sterilization techniques inspected." Asian Journal of Medical Sciences 14, no. 11 (2023): 202–8. http://dx.doi.org/10.3126/ajms.v14i11.56419.

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Background: Atypical wound infections following minimal access surgery continue to affect smooth recovery. Percutaneous nephrolithotomy (PCNL) for renal stones involves various small-sized nephroscopes and accessories. Improper cleaning and disinfection of which may result in bacterial biofilms formation and infections. Aims and Objectives: We inspected an outbreak of atypical wound infection after PCNL to find the responsible factor. Materials and Methods: This retrospective observational study had three groups based on cleaning and sterilization methods. In group A, manual cleaning of instru

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Maartens, A., P. Swart, and E. P. Jacobs. "An enzymatic approach to the cleaning of ultrafiltration membranes fouled in abattoir effluent." Journal of Membrane Science 119, no. 1 (1996): 9–16. http://dx.doi.org/10.1016/0376-7388(96)00015-4.

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Irfan, Sarah, Seema Irfan, Mubassar Fida, and Israr Ahmad. "Contamination assessment of orthodontic bands after different pre-cleaning methods at a tertiary care hospital." Journal of Orthodontics 46, no. 3 (2019): 220–24. http://dx.doi.org/10.1177/1465312519855402.

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Introduction: Infection control in dentistry is a major concern due to risk of transmission of communicable diseases. The aim of this study is to evaluate and compare the efficacy of various pre-cleaning methods for the tried-in orthodontic bands. Material and methods: An in-vitro experimental study was conducted at the Central Sterilization Services Department (Dental Clinic) and the Microbiology lab at our university hospital. A total of 130 bands were included in our study which comprised 10 controls and the rest were equally divided into three groups according to the pre-cleaning methods,

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Smith, Edward, Q. Zhang, B. Farrand, V. Kokol, and Jin Song Shen. "The Development of a Bio-Scouring Process for Raw Wool Using Protease." Advanced Materials Research 441 (January 2012): 10–15. http://dx.doi.org/10.4028/www.scientific.net/amr.441.10.

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The use of protease in the raw wool scouring process was investigated. Both native protease and an enlarged protease prepared by chemical modification were used. It was demonstrated that enzymatic treatment with protease in the scouring process (bio-scouring) can achieve cleaning of the fibre and modification of the cuticle layer leading to shrink-resistance. A reduction of lipid content was found and led to an improvement in dyeability of the fibre.

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Alfa, Michelle J., Harminder Singh, Zoann Nugent, et al. "Simulated-Use Polytetrafluorethylene Biofilm Model: Repeated Rounds of Complete Reprocessing Lead to Accumulation of Organic Debris and Viable Bacteria." Infection Control & Hospital Epidemiology 38, no. 11 (2017): 1284–90. http://dx.doi.org/10.1017/ice.2017.215.

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OBJECTIVEBiofilm has been implicated in bacterial persistence and survival after endoscope reprocessing. In this study, we assessed the impact of different methods of reprocessing on organic residues and viable bacteria after repeated rounds of biofilm formation when each was followed by full reprocessing.METHODSATS-2015, an artificial test soil containing 5–8 Log10 colony-forming units (CFU) of Enterococcus faecalis and Pseudomonas aeruginosa, was used to form biofilm in polytetrafluroethylene channels overnight on 5 successive days. Each successive day, full pump-assisted cleaning using bris

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Song, Ji Eun, Su Mi Kim, and Hye Rim Kim. "Improvement of dye affinity in natural dyeing using Terminalia chebula retzius (T. chebula) applied to leather." International Journal of Clothing Science and Technology 29, no. 5 (2017): 610–26. http://dx.doi.org/10.1108/ijcst-03-2017-0029.

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Purpose The purpose of this paper is to improve the dye affinity of natural dye of Terminalia chebula retzius (T. chebula) using the dye substrate of leather. Design/methodology/approach The dyeing conditions such as temperature, concentration of dye, and time are controlled by measuring the dye affinity. The effect of enzymatic post-tanning process on dye affinity is evaluated by using different type of proteases such as flavourzyme, alcalase, and bromelain. The optimum conditions for enzymatic post-tanning process are evaluated depending on different pH, temperature, and concentration of enz

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Wehner, F., L. Garretson, K. Dawson, Y. Segal, and L. Reuss. "A nonenzymatic preparation of epithelial basolateral membrane for patch clamp." American Journal of Physiology-Cell Physiology 258, no. 6 (1990): C1159—C1164. http://dx.doi.org/10.1152/ajpcell.1990.258.6.c1159.

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A preparation has been developed that permits patch clamping of the basolateral membrane of Necturus gallbladder epithelial cells with a high success rate. The epithelium is separated from the underlying tissues mechanically, without enzymatic treatment. Its apical surface is attached to a plastic cover slip, and the basolateral surface, facing up, is cleaned with a suction pipette under microscopic observation. With this cleaning procedure, the success rate in obtaining gigaohm seals increases from less than 1% to approximately 10% of the attempts. The cells appear to retain their structural

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Nadjafova, S. I., and F. S. Keyserukhskaya. "Investigation of the Number and Enzymatic Activity of Microorganisms in Soils Contaminated with Pesticides." Агрохимия, no. 3 (June 16, 2024): 61–65. http://dx.doi.org/10.31857/s0002188124030086.

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The results of a study of the number and enzymatic activity of microorganisms in the soils of the Zardob district (Azerbaijan), from the territory of the pesticide storage base, are presented. It was revealed that the soils of the base were polluted to a very strong extent, and the content of pesticides (including DDT) exceeded the maximum permissible concentration (MPC) by tens to hundreds of times. Microbiological studies of soil samples showed that, unlike pure soil, in all soil samples contaminated with pesticides, the number of microorganisms and enzymatic activity decreased, which indica

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Allen, George. "Enzymatic detergent use for gastroscope cleaning; surgical hand disinfectants; instrument decontamination; residual protein levels." AORN Journal 84, no. 5 (2006): 863–68. http://dx.doi.org/10.1016/s0001-2092(06)63973-2.

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Muñoz-Aguado, M. J., D. E. Wiley, and A. G. Fane. "Enzymatic and detergent cleaning of a polysulfone ultrafiltration membrane fouled with BSA and whey." Journal of Membrane Science 117, no. 1-2 (1996): 175–87. http://dx.doi.org/10.1016/0376-7388(96)00066-x.

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Lyu, Bin, Kun Cheng, Jianzhong Ma, et al. "A cleaning and efficient approach to improve wet-blue sheepleather quality by enzymatic degreasing." Journal of Cleaner Production 148 (April 2017): 701–8. http://dx.doi.org/10.1016/j.jclepro.2017.01.170.

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Liu, Jinwei, Weichang Xu, Feng Yan, Xinqi Liu, and Yuan Wang. "Cleaning Process of Diosgenin from Dioscorea nipponica." Journal of Biobased Materials and Bioenergy 18, no. 1 (2024): 134–41. http://dx.doi.org/10.1166/jbmb.2024.2335.

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This investigation employed HPLC and LC-MS techniques to elucidate the enzymolysis and acid hydrolysis mechanisms of diosgenin obtained through a cleaning process. The findings revealed that the enzymolysis led to the cleavage and subsequent recombination of the glycosidic bond at the C-26 position of protodioscin, resulting in the formation of dioscin present in the enzymatic hydrolysis filter residue. Leveraging this observation, a streamlined and eco-friendly method for diosgenin extraction was devised. Incorporating the Box-Behnken response surface methodology alongside wastewater assessme

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Klein, Allan E., John Freiberg, Steven Same, and Maryanne Carroll. "Rapid Colorimetric Determination of Activity of Subtilisin Enzymes in Cleaning Products." Journal of AOAC INTERNATIONAL 72, no. 6 (1989): 881–82. http://dx.doi.org/10.1093/jaoac/72.6.881.

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Abstract A new colorimetric method is described for the determination of enzymatic activity of subtilisin in cleaning products. The procedure is more rapid and precise than the casein digestion methods commonly used to assay protease activity. The principle of the colorimetric method depends on the determination rate of p-nitrophenol released on hydrolysis of N-CBZ-L-leucine-/Miitrophenyl ester at pH 8.0 by subtilisin, with correction for any nonenzymatic (spontaneous) hydrolysis of the substrate. Because of the broad range of hydrolytic activity of this enzyme, and the difficulties in predict

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Collins, William O. "A Review of Reprocessing Techniques of Flexible Nasopharyngoscopes." Otolaryngology–Head and Neck Surgery 141, no. 3 (2009): 307–10. http://dx.doi.org/10.1016/j.otohns.2009.05.027.

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To provide assistance to otolaryngologists to decide the best manner in which to reprocess flexible nasopharyngoscopes, a review of existing English language medical literature regarding the methods of flexible endoscope reprocessing was performed, including previously published guidelines from other medical disciplines. Multiple steps were confirmed to be critical to effectively reprocess flexible nasopharyngoscopes. High-level disinfection has been determined to be the minimum level of disinfection required for reprocessing of flexible nasopharyngoscopes. Several steps are important in all r

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Alekseyev, Andrey A., Alexander E. Bobrovnikov, Natalia B. Malyutina, Vitaly V. Bogdanov, Nikolai M. Chilikin, and Arnold E. Markarov. "Effectiveness of Enzymatic Necrolysis in Patients with Burns." Journal of Experimental and Clinical Surgery 17, no. 4 (2024): 183–91. https://doi.org/10.18499/2070-478x-2024-17-4-183-191.

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Rationale. Currently, there has been developed a technique for managing enzymatic necrolysis of II-III degree burn wounds using the two-component drug NexoBrid; this treatment option is possible to apply only in the early terms, up to 72 hours, after injury. There are no indications for the use of the technology in later terms after the injury. The aim of research was to study the potential and effectiveness of enzymatic necrolysis when using the drug NexoBrid in patients with burns of II-III degree at various stages after injury (before and after 72 hours). Methods. The study included 23 pati

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Colantonio, Claudia, Luca Lanteri, Ramona Bocci, Valeria Valentini, and Claudia Pelosi. "“A Woman Clothed with the Sun”: The Diagnostic Study and Testing of Enzyme-Based Green Products for the Restoration of an Early 17th Century Wall Painting in the Palazzo Gallo in Bagnaia (Italy)." Applied Sciences 13, no. 23 (2023): 12884. http://dx.doi.org/10.3390/app132312884.

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A 17th century wall painting, representing the Virgin between two Saints, in a noble Italian renaissance palace, the Palazzo Gallo in Bagnaia (Viterbo, Italy), was restored in 2021 in the context of a wider restoration campaign involving the main room of the palace built by cardinal Sansoni Riario. Diagnostic analyses performed using traditional characterization techniques (optical microscopy on micro-stratigraphic sections, X-ray fluorescence spectroscopy and Fourier transform infrared spectroscopy) provided the identification of both the original painting and its restoration materials, while

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