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Journal articles on the topic 'Enzymatic detachment'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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1

Kaplan, Jeffrey B., Chandran Ragunath, Kabilan Velliyagounder, Daniel H. Fine, and Narayanan Ramasubbu. "Enzymatic Detachment of Staphylococcus epidermidis Biofilms." Antimicrobial Agents and Chemotherapy 48, no. 7 (2004): 2633–36. http://dx.doi.org/10.1128/aac.48.7.2633-2636.2004.

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ABSTRACT The gram-positive bacterium Staphylococcus epidermidis is the most common cause of infections associated with catheters and other indwelling medical devices. S. epidermidis produces an extracellular slime that enables it to form adherent biofilms on plastic surfaces. We found that a biofilm-releasing enzyme produced by the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans rapidly and efficiently removed S. epidermidis biofilms from plastic surfaces. The enzyme worked by releasing extracellular slime from S. epidermidis cells. Precoating surfaces with the enzyme p

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Salzig, Denise, Alexandra Schmiermund, Pablo P. Grace, Christiane Elseberg, Christian Weber, and Peter Czermak. "Enzymatic Detachment of Therapeutic Mesenchymal Stromal Cells Grown on Glass Carriers in a Bioreactor." Open Biomedical Engineering Journal 7, no. 1 (2013): 147–58. http://dx.doi.org/10.2174/1874120701307010147.

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Cell therapies require the in vitro expansion of adherent cells such as mesenchymal stromal cells (hMSCs) in bioreactor systems or other culture environments, followed by cell harvest. As hMSCs are strictly adherent cells, cell harvest requires cell detachment. The use of hMSCs for cell therapy requires GMP production in accordance with the guidelines for advanced therapeutic medical products. Therefore, several GMP-conform available proteolytic enzymes were investigated for their ability to promote hMSC detachment. An allogeneic hMSC cell line (hMSC-TERT) that is used in clinical trials in th

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Inoue, Kanako, Takatoshi Onoe, Pyoyun Park, and Kenichi Ikeda. "Enzymatic detachment of spore germlings in Magnaporthe oryzae." FEMS Microbiology Letters 323, no. 1 (2011): 13–19. http://dx.doi.org/10.1111/j.1574-6968.2011.02353.x.

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4

Wan, Ran, Thomas Hong, Yasser Tariq, and Andrew Chang. "Pharmacotherapy of Vitreomacular Traction." Current Pharmaceutical Design 24, no. 41 (2019): 4874–81. http://dx.doi.org/10.2174/1381612825666190124102148.

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Vitreomacular traction occurs due to incomplete or anomalous posterior vitreous detachment. Over time, the vitreous pulls anteriorly and causes retinal distortion and eventually reduced vision. Traditionally, vitreomacular traction was treated with vitrectomy surgery. In the past few years, there is a paradigm shift towards pharmacologic vitreolysis, which involves the intravitreal injection of enzymatic and non-enzymatic agents that facilitate posterior vitreous detachment. Many agents have been investigated and trialled including plasmin, microplasmin (Ocriplasmin), hyaluronidase, nattokinas

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Tultseva, Svetlana Nikolaevna. "The enzymatic vitreolysis — a new trend in the treatment of retinal vein occlusions." Ophthalmology journal 6, no. 1 (2013): 35–43. http://dx.doi.org/10.17816/ov2013135-43.

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Vitreoretinal relationships exert a significant influence on the course of retinal vein occlusions (RVO) and post-thrombotic retinopathy. A single intravitreal injection of Actilyse 50 mkg in the period from 1 till 21 days from the disease onset stimulates a posterior vitreous detachment in 30.4% patients with non-ischemic and 24.8% patients with the type of ischemic RVO. The process of transition from the partial posterior hyaloid detachment to the complete one, and complete posterior hyaloid detachment in patients who did not have it from the beginning, was accompanied by a visual acuity inc

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Kim, Yu-Jin, Tae-Jin Lee, Gun-Jae Jeong, et al. "Development of pH-Responsive Polymer Coating as an Alternative to Enzyme-Based Stem Cell Dissociation for Cell Therapy." Materials 14, no. 3 (2021): 491. http://dx.doi.org/10.3390/ma14030491.

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Cell therapy usually accompanies cell detachment as an essential process in cell culture and cell collection for transplantation. However, conventional methods based on enzymatic cell detachment can cause cellular damage including cell death and senescence during the routine cell detaching step due to an inappropriate handing. The aim of the current study is to apply the pH-responsive degradation property of poly (amino ester) to the surface of a cell culture dish to provide a simple and easy alternative method for cell detachment that can substitute the conventional enzyme treatment. In this

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Christwardana, Marcelinus, and Domenico Frattini. "Electrochemical Study of Enzymatic Glucose Sensors Biocatalyst: Thermal Degradation after Long-Term Storage." Chemosensors 6, no. 4 (2018): 53. http://dx.doi.org/10.3390/chemosensors6040053.

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The thermal degradation related to stability in long-term storage of a carbon nanotube-based biosensor has been investigated. The effect of storage temperature on detachment and denaturation of glucose oxidase (GOx) biocatalyst has been proved. The carbon nanotubes (CNTs) coated with polyethyleneimine (PEI) as entrapping polymer to attract more GOx to form a durable and layered CNT/PEI/GOx structure is used for long-term storage to minimize GOx detachment from the structure and minimize the possibility of enzyme and protein denaturation. After 120 days, the glucose response of the CNT/PEI/GOx

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8

Lee, S. F., Y. H. Li, and G. H. Bowden. "Detachment of Streptococcus mutans biofilm cells by an endogenous enzymatic activity." Infection and immunity 64, no. 3 (1996): 1035–38. http://dx.doi.org/10.1128/iai.64.3.1035-1038.1996.

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9

Rovere, Marie-Rose, Patricia Rousselle, Marek Haftek, et al. "Preserving Basement Membranes during Detachment of Cultivated Oral Mucosal Epithelial Cell Sheets for the Treatment of Total Bilateral Limbal Stem Cell Deficiency." Cell Transplantation 27, no. 2 (2018): 264–74. http://dx.doi.org/10.1177/0963689717741140.

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Total bilateral limbal stem cell deficiency leading to loss of corneal clarity, potential vision loss, pain, photophobia, and keratoplasty failure cannot be treated by autologous limbal transplantation, and allogeneic limbal transplantation requires subsequent immunosuppressive treatment. Cultured autologous oral mucosal epithelial cells have been shown to be safe and effective alternatives. These cells can be transplanted on supports or without support after detachment from the culture dishes. Dispase, known for epidermal sheet detachment, is reported as not usable for oral mucosa. The object

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10

Böckelmann, Uta, Ulrich Szewzyk, and Elisabeth Grohmann. "A new enzymatic method for the detachment of particle associated soil bacteria." Journal of Microbiological Methods 55, no. 1 (2003): 201–11. http://dx.doi.org/10.1016/s0167-7012(03)00144-1.

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11

Uzdensky, Anatoly, Asta Juzeniene, Li-Wei Ma, and Johan Moan. "Photodynamic inhibition of enzymatic detachment of human cancer cells from a substratum." Biochimica et Biophysica Acta (BBA) - General Subjects 1670, no. 1 (2004): 1–11. http://dx.doi.org/10.1016/j.bbagen.2003.10.013.

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12

Laurent, Patrick, and Pierre Servais. "Fixed bacterial biomass estimated by potential exoproteolytic activity." Canadian Journal of Microbiology 41, no. 8 (1995): 749–52. http://dx.doi.org/10.1139/m95-103.

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A method is proposed to estimate the bacterial biomass fixed on solid supports colonized in water without detachment of the cells from the supports. The method consists of measuring an enzymatic activity of the fixed bacteria: the potential exoproteolytic activity, which has been shown to be proportional to the bacterial biomass.Key words: fixed bacterial biomass, support colonization, exoproteolytic activity.

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13

HAROONI, MARK, TOD McMILLAN, and MIGUEL REFOJO. "EFFICACY AND SAFETY OF ENZYMATIC POSTERIOR VITREOUS DETACHMENT BY INTRAVITREAL INJECTION OF HYALURONIDASE." Retina 18, no. 1 (1998): 16–22. http://dx.doi.org/10.1097/00006982-199818010-00004.

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14

HAROONI, MARK, TOD McMILLAN, and MIGUEL REFOJO. "EFFICACY AND SAFETY OF ENZYMATIC POSTERIOR VITREOUS DETACHMENT BY INTRAVITREAL INJECTION OF HYALURONIDASE." Retina 18, no. 1 (1998): 16–22. http://dx.doi.org/10.1097/00006982-199801000-00004.

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15

Kudo, H., K. J. Cheng, and J. W. Costerton. "Electron microscopic study of the methylcellulose-mediated detachment of cellulolytic rumen bacteria from cellulose fibers." Canadian Journal of Microbiology 33, no. 3 (1987): 267–72. http://dx.doi.org/10.1139/m87-045.

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The presence of methylellulose prevents the attachment of cellulolytic rumen bacteria to cellulose fibers. The addition of methylcellulose to pure cultures of these organisms in which the cells are already adherent to cellulose causes their detachment from this insoluble substrate and the inhibition of their growth. Methylcellulose is not used as a carbon source by these organisms and has no effect on their growth when glucose and cellobiose are the carbon sources. Attached cells of Bacteroides succinogenes orient themselves in the plane of the individual cellulose fibers and their methylcellu

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16

Büks, Frederick, and Martin Kaupenjohann. "Enzymatic biofilm digestion in soil aggregates facilitates the release of particulate organic matter by sonication." SOIL 2, no. 4 (2016): 499–509. http://dx.doi.org/10.5194/soil-2-499-2016.

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Abstract. The stability of soil aggregates against shearing and compressive forces as well as water-caused dispersion is an integral marker of soil quality. High stability results in less compaction and erosion and has been linked to enhanced water retention, dynamic water transport and aeration regimes, increased rooting depth, and protection of soil organic matter (SOM) against microbial degradation. In turn, particulate organic matter is supposed to support soil aggregate stabilization. For decades the importance of biofilm extracellular polymeric substances (EPSs) regarding particulate org

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17

Sit, K. H., K. P. Wong, and B. H. Bay. "An efficient and simple method of non-enzymatic detachment of monolayer cultures into single cell suspension." Journal of Tissue Culture Methods 13, no. 4 (1991): 257–59. http://dx.doi.org/10.1007/bf02388258.

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18

Aryal, Manish, Preetty Pranatharthiharan, and Peter M. Muriana. "Optimization of a Microplate Assay for Generating Listeria Monocytogenes, E. Coli O157:H7, and Salmonella Biofilms and Enzymatic Recovery for Enumeration." Foods 8, no. 11 (2019): 541. http://dx.doi.org/10.3390/foods8110541.

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Biofilms enable the persistence of pathogens in food processing environments. Sanitizing agents are needed that are effective against pathogens entrapped in biofilms that are more difficult to inactivate than planktonic cells that are displaced and found on equipment surfaces. We examined conditions to develop, analyze, and enumerate the enhanced biofilms of three different foodborne pathogens assisted by fluorescence adherence assay and enzymatic detachment. We compared three different isomeric forms of fluorescent substrates that are readily taken up by bacterial cells based on carboxy-fluor

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19

Arroyo, C. M., R. J. Schafer, E. M. Kurt, C. A. Broomfield, and A. J. Carmichael. "Response of normal human keratinocytes to sulfur mustard (HD): cytokine release using a non-enzymatic detachment procedure." Human & Experimental Toxicology 18, no. 1 (1999): 1–11. http://dx.doi.org/10.1177/096032719901800101.

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Cytokines play a major role in both acute and chronic inflammatory processes, including those produced by sulfur mustard (HD). This study describes responses of normal human epidermal keratinocyte (NHEK) cells to 2,2′-dichlorodiethyl sulfide, sulfur mustard (HD), defined by interleukin-1 beta (IL-1β), interleukin-6 (IL-6), inter-leukin-8 (IL-8), and tumor necrosis factor-alpha (TNF-α) release. A new method for detaching cell to cell adhesion between keratinocytes has been applied. This method permits the characterization of endogenous fluid from cellular content that could be applied for the d

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20

Arroyo, C. M., R. J. Schafer, E. M. Kurt, C. A. Broomfield, and A. J. Carmichael. "Response of normal human keratinocytes to sulfur mustard (HD): cytokine release using a non-enzymatic detachment procedure." Human & Experimental Toxicology 18, no. 1 (1999): 1–11. http://dx.doi.org/10.1191/096032799678839329.

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21

WANG, DANHUI, ZIYUAN WANG, FEI HE, AMANDA J. KINCHLA, and SAM R. NUGEN. "Enzymatic Digestion for Improved Bacteria Separation from Leafy Green Vegetables." Journal of Food Protection 79, no. 8 (2016): 1378–86. http://dx.doi.org/10.4315/0362-028x.jfp-15-581.

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ABSTRACT An effective and rapid method for the separation of bacteria from food matrix remains a bottleneck for rapid bacteria detection for food safety. Bacteria can strongly attach to a food surface or internalize within the matrix, making their isolation extremely difficult. Traditional methods of separating bacteria from food routinely involve stomaching, blending, and shaking. However, these methods may not be efficient at removing all the bacteria from complex matrices. Here, we investigate the benefits of using enzyme digestion followed by immunomagnetic separation to isolate Salmonella

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22

Canavan, Heather E., Xuanhong Cheng, Daniel J. Graham, Buddy D. Ratner, and David G. Castner. "Cell sheet detachment affects the extracellular matrix: A surface science study comparing thermal liftoff, enzymatic, and mechanical methods." Journal of Biomedical Materials Research Part A 75A, no. 1 (2005): 1–13. http://dx.doi.org/10.1002/jbm.a.30297.

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23

Mulder, AB, KS Hegge-Paping, CP Magielse, et al. "Tumor necrosis factor alpha-induced endothelial tissue factor is located on the cell surface rather than in the subendothelial matrix." Blood 84, no. 5 (1994): 1559–66. http://dx.doi.org/10.1182/blood.v84.5.1559.1559.

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Abstract Because there is no consensus regarding the precise distribution of induced endothelial tissue factor (TF), we studied TF activity in and on tumor necrosis factor alpha-stimulated cultured human umbilical vein endothelial cells (ECs) and their underlying matrix. TF was mainly expressed on the cell surface. Only small traces were found on the apical surface suggesting that TF is predominantly located on the basolateral side of the cell membrane. The presence of TF on the cell surface was confirmed by flow cytometry. Subendothelial TF activity appeared to be dependent upon the procedure

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24

Mulder, AB, KS Hegge-Paping, CP Magielse, et al. "Tumor necrosis factor alpha-induced endothelial tissue factor is located on the cell surface rather than in the subendothelial matrix." Blood 84, no. 5 (1994): 1559–66. http://dx.doi.org/10.1182/blood.v84.5.1559.bloodjournal8451559.

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Because there is no consensus regarding the precise distribution of induced endothelial tissue factor (TF), we studied TF activity in and on tumor necrosis factor alpha-stimulated cultured human umbilical vein endothelial cells (ECs) and their underlying matrix. TF was mainly expressed on the cell surface. Only small traces were found on the apical surface suggesting that TF is predominantly located on the basolateral side of the cell membrane. The presence of TF on the cell surface was confirmed by flow cytometry. Subendothelial TF activity appeared to be dependent upon the procedure used to

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25

Koivulehto, Marianne, Natalia Battchikova, Saara Korpela, Elvira Khalikova, Anton Zavialov, and Timo Korpela. "Comparison of kinetic and enzymatic properties of intracellular phosphoserine aminotransferases from alkaliphilic and neutralophilic bacteria." Open Chemistry 18, no. 1 (2020): 149–64. http://dx.doi.org/10.1515/chem-2020-0014.

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AbstractIntracellular pyridoxal 5´-phosphate (PLP) -dependent recombinant phosphoserine aminotransferases (PSATs; EC 2.6.1.52) from two alkaliphilic Bacillus strains were overproduced in Escherichia coli, purified to homogeneity and their enzymological characteristics were compared to PSAT from neutralophilic E. coli. Some of the enzymatic characteristics of the PSATs from the alkaliphiles were unique, showing high and sharp pH optimal of the activity related to putative internal pH inside the microbes. The specific activities of all of the studied enzymes were similar (42-44 U/mg) as measured

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Rennert, Knut, Mirko Nitschke, Maria Wallert, et al. "Thermo-responsive cell culture carrier: Effects on macrophage functionality and detachment efficiency." Journal of Tissue Engineering 8 (January 1, 2017): 204173141772642. http://dx.doi.org/10.1177/2041731417726428.

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Harvesting cultivated macrophages for tissue engineering purposes by enzymatic digestion of cell adhesion molecules can potentially result in unintended activation, altered function, or behavior of these cells. Thermo-responsive polymer is a promising tool that allows for gentle macrophage detachment without artificial activation prior to subculture within engineered tissue constructs. We therefore characterized different species of thermo-responsive polymers for their suitability as cell substrate and to mediate gentle macrophage detachment by temperature shift. Primary human monocyte- and TH

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Heruye, Segewkal H., Leonce N. Maffofou Nkenyi, Neetu U. Singh, et al. "Current Trends in the Pharmacotherapy of Cataracts." Pharmaceuticals 13, no. 1 (2020): 15. http://dx.doi.org/10.3390/ph13010015.

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Cataracts, one of the leading causes of preventable blindness worldwide, refers to lens degradation that is characterized by clouding, with consequent blurry vision. As life expectancies improve, the number of people affected with cataracts is predicted to increase worldwide, especially in low-income nations with limited access to surgery. Although cataract surgery is considered safe, it is associated with some complications such as retinal detachment, warranting a search for cheap, pharmacological alternatives to the management of this ocular disease. The lens is richly endowed with a complex

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28

Suttorp, N., C. Galanos, and H. Neuhof. "Endotoxin alters arachidonate metabolism in pulmonary endothelial cells." American Journal of Physiology-Cell Physiology 253, no. 3 (1987): C384—C390. http://dx.doi.org/10.1152/ajpcell.1987.253.3.c384.

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Endotoxin and lipid A dose dependently (1 ng/ml to 10 micrograms/ml) and time dependently (6-24 h) stimulated the generation of large amounts of prostacyclin in cultured pig pulmonary artery endothelial cells. This effect occurred in the absence of cell detachment and overt cell damage. The presence of at least 1% serum was required but the activation of the complement cascade was not. Endotoxin-treated endothelial cells generated increased amounts of prostacyclin upon stimulation with A23187 and arachidonic acid. Endotoxin-induced activation of arachidonate metabolism could be reduced by 10(-

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Niederhafner, Petr, Martin Šafařík, Jaroslav Šebestík, Vladimír Gut, Petr Maloň, and Jan Hlaváček. "Synthesis of protected peptides from the human IgG1 hinge region on PEG support using disulfide bond synthons and alkaline or enzymatic detachment." Tetrahedron Letters 47, no. 6 (2006): 1023–25. http://dx.doi.org/10.1016/j.tetlet.2005.11.135.

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30

Yang, Lei, Xiaoguang Fan, Jing Zhang, and Jia Ju. "Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications." Polymers 12, no. 2 (2020): 389. http://dx.doi.org/10.3390/polym12020389.

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Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote “on–off” behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by

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31

Jankiewicz, Leszek S. "Machinism of abscission of leaves and reproductive parts of plants - a model." Acta Societatis Botanicorum Poloniae 54, no. 3 (2014): 285–322. http://dx.doi.org/10.5586/asbp.1985.027.

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Abscission of leaves and reproductive parts of plants is presented in the form of a model which allows the reader to see the process as a whole. The process of abscission was divided into 2 parts: the first of which may be inhibited or reversed by auxin, and the second, which is mostly irreversible. Increased ethylene (ET) production links the earlier phases of senescence with abscission. ET has a double role in the initiation of the abscission process: (1) acting on the leaf blade, it withholds the export of auxin from a leaf, in consequence the abscission zone (AZ) is scarcely supplied with

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Garay-Canales, Claudia A., Ileana Licona-Limón, and Enrique Ortega. "Distinct Epitopes on CD13 Mediate Opposite Consequences for Cell Adhesion." BioMed Research International 2018 (2018): 1–17. http://dx.doi.org/10.1155/2018/4093435.

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CD13 is a membrane glycoprotein with aminopeptidase activity, expressed on several cell types, including myeloid cells (dendritic cells, monocytes, macrophages, neutrophils, etc.). CD13 participates in several functions such as proteolytic regulation of bioactive peptides, viral receptor, angiogenesis, and tumor metastasis. CD13 has also been proposed to participate in cell adhesion, as crosslinking of CD13 by certain CD13-specific antibodies induces homotypic aggregation of monocytes and heterotypic adhesion of monocytes to endothelial cells. We generated two monoclonal antibodies (mAbs C and

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Zhang, Qingsong, Rui Xue, Xuewei Li, Zheng Wang, Zhiyi Zhou, and Li Chen. "Silk sericin/poly (NIPAM/LMSH) nanocomposite hydrogels: Rapid thermo-responsibility and good carrier for cell proliferation." Pure and Applied Chemistry 86, no. 5 (2014): 721–31. http://dx.doi.org/10.1515/pac-2013-1214.

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AbstractTo overcome the fragility of chemical cross-linked hydrogel and improve the cell compatibility of poly(N-isopropylacrylamide) hydrogel, physical cross-linker lithium magnesium silicate hydrate (LMSH) and silk sericin (SS) created by Bombyx mori (silkworms) were adopted as physical cross-linker and biocompatible monomer to prepare SS/poly(NIPAM/LMSH) nanocomposite hydrogels. The temperature sensitivity and cytocompatibility like mouse ﬁbroblasts (L929) cell proliferation and desorption were investigated. Depending on SS content, the resultant nanocomposite hydrogels present bigger volum

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Panicker, Neena, Sameera Balhamar, Shaima Akhlaq, et al. "Identification and Characterization of the Caspase-Mediated Apoptotic Activity of Teucrium mascatense and an Isolated Compound in Human Cancer Cells." Molecules 24, no. 5 (2019): 977. http://dx.doi.org/10.3390/molecules24050977.

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Plants of the genus Teucrium (Lamiaceae or Labiatae family) are known historically for their medicinal value. Here, we identify and characterize the anticancer potential of T. mascatense and its active compound, IM60, in human cancer cells. The anti-proliferative effect of a T. mascatense methanol extract and its various fractions were analyzed in MCF-7 and HeLa cells in a dose- and time dependent manner. The dichloromethane fraction (TMDF) was observed to be the most effective with cytotoxicity against a more expanded series of cell lines, including MDA-MB-231. A time and dose-dependent toxic

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Vutukuru, S., Ch Suma, K. Madhavi, et al. "Studies on the Development of Potential Biomarkers for Rapid Assessment of Copper Toxicity to Freshwater Fish using Esomus danricus as Model." International Journal of Environmental Research and Public Health 2, no. 1 (2005): 63–73. http://dx.doi.org/10.3390/ijerph2005010063.

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Living in an environment that has been altered considerably by anthropogenic activities, fish are often exposed to a multitude of stressors including heavy metals. Copper ions are quite toxic to fish when concentrations are increased in environmental exposures often resulting in physiological, histological, biochemical and enzymatic alterations in fish, which have a great potential to serve as biomarkers. Esomus danricus was chosen as model in the present study and the metabolic rate, gill morphology, total glycogen, total protein, superoxide dismutase and catalase were critically evaluated. T

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Faut, Mónica, Carmen Rodríguez de Castro, Florencia M. Bietto, José A. Castro, and Gerardo D. Castro. "Metabolism of ethanol to acetaldehyde and increased susceptibility to oxidative stress could play a role in the ovarian tissue cell injury promoted by alcohol drinking." Toxicology and Industrial Health 25, no. 8 (2009): 525–38. http://dx.doi.org/10.1177/0748233709345937.

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It is known that drinking alcohol can lead to reproductive problems in women. In this study, we analyzed the possibility that part of those effects were mediated through alterations of ovarian function related to ethanol oxidation to acetaldehyde occurring in situ. Biotransformation in the rat ovary cytosolic fraction was partially inhibited by allopurinol, suggesting the participation of xanthine oxidoreductase in the process. Microsomal pathway was of enzymatic nature, requiring nicotinamide adenine dinucleotide phosphate-oxidase (NADPH), sensitive to oxygen and significantly inhibited by so

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Holbrook, K. A. "Structure, compositon and function of the dermal-epidermal junction: Relevance in cutaneous disease and diagnosis." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 868–69. http://dx.doi.org/10.1017/s0424820100156328.

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The dermal-epidermal junction (DEJ), or basement membrane rone, is the boundary between the epithelial and mesenchymal compartments of the skin; epidermal and fibroblastic cells in these two regions collaborate to synthesire its components. Ultrastructural studies (TEM and SEM) have defined a series of planes or layers (basal epidermal, lamina lucida, lamina densa, sublamina densa) and the morphology and density of attachment structures (hemidesmosomes, anchoring filaments, anchoring fibrils and anchoring plaques) in this region of normal skin. Change in structure of the DEJ provides informati

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Terao, Yusuke, Yuta Kurashina, Shugo Tohyama, et al. "An effective detachment system for human induced pluripotent stem cells cultured on multilayered cultivation substrates using resonance vibrations." Scientific Reports 9, no. 1 (2019). http://dx.doi.org/10.1038/s41598-019-51944-w.

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Abstract Clinical application of human induced pluripotent stem cells (hiPSCs) has been hampered by the lack of a practical, scalable culture system. Stacked culture plates (SCPs) have recently attracted attention. However, final cell yields depend on the efficiency of cell detachment, and inefficient cell recovery from SCPs presents a major challenge to their use. We have developed an effective detachment method using resonance vibrations (RVs) of substrates with sweeping driving frequency. By exciting RVs that have 1–3 antinodes with ultra-low-density enzyme spread on each substrate of SCPs,

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van Luyn, M. J. A., P. B. van Wachem, L. H. H. Olde Damink, P. J. Dijkstra, J. Feijen, and P. Nieuwenhuis. "Enzyme-Pretreatment Removes In Vitro Cytoxic Effects of Dermal Sheep Collagen." MRS Proceedings 252 (1991). http://dx.doi.org/10.1557/proc-252-167.

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ABSTRACTWe investigated the in vitro cytotoxicity of commercially available hexamethylenediisocyanate-crosslinked dermal sheep collagen (HDSC). HDSC was found to induce medium cytotoxic effects, as measured with methylcellulose cell culture. Apart from primary cytotoxicity, due to direct release of (extractable) cytotoxic products, HDSC was found to contain secondary cytotoxicity, possibly released by enzymatic interactions. In this study we found proof for this hypothesis, by exposing extracted HDSC to enzyme-containing medium. Furthermore we observed, that enzymatic pre-treatment can remove

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Khan, Mohammad Imran, Mazin A. Zamzami, Aftab Ahmad, and Hani Choudhry. "Molecular profiling of epigenetic landscape of cancer cells during extracellular matrix detachment." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-021-82431-w.

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AbstractDuring cancer, a major challenge faced by oncologists is the treatment of metastasis; a leading cause of cancer-related deaths around the world. Metastasis involves a highly ordered sequence of events starting with the detachment of tumor cells from the extracellular matrix (E.C.M.). In normal cells, detachment from E.C.M. triggers programmed cell death, termed anoikis. However, tumor cells dodge their way to anoikis and spread to distant sites for initiating the metastatic program. In this work, we explored the impact of E.C.M. detachment on the expression of some major oncogenic hist

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Griffiths, Helen Jane, John G. Harvey, James Dean, James A. Curran, Athina E. Markaki, and T. William Clyne. "Characterisation of Cell Adhesion to Substrate Materials and the Resistance to Enzymatic and Mechanical Cell-Removal." MRS Proceedings 1097 (2008). http://dx.doi.org/10.1557/proc-1097-gg03-05.

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AbstractCell-implant adhesive strength is important for prostheses. In this paper, an investigation is described into the adhesion of bovine chondrocytes to Ti6Al4V-based substrates with different surface roughnesses and compositions. Cells were cultured for 2 or 5 days, to promote adhesion. The ease of cell removal was characterised, using both biochemical (trypsin) and mechanical (accelerated buoyancy and liquid flow) methods. Computational fluid dynamics (CFD) modelling has been used to estimate the shear forces applied to the cells by the liquid flow. A comparison is presented between the

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Santra, Mithun, Maryada Sharma, Deeksha Katoch, et al. "Induction of posterior vitreous detachment (PVD) by non-enzymatic reagents targeting vitreous collagen liquefaction as well as vitreoretinal adhesion." Scientific Reports 10, no. 1 (2020). http://dx.doi.org/10.1038/s41598-020-64931-3.

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Santra, Mithun, Maryada Sharma, Deeksha Katoch, et al. "Author Correction: Induction of posterior vitreous detachment (PVD) by non-enzymatic reagents targeting vitreous collagen liquefaction as well as vitreoretinal adhesion." Scientific Reports 10, no. 1 (2020). http://dx.doi.org/10.1038/s41598-020-69093-w.

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Nakao, Mitsuyoshi, Kyungsook Kim, Kenichi Nagase, David W. Grainger, Hideko Kanazawa, and Teruo Okano. "Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets." Stem Cell Research & Therapy 10, no. 1 (2019). http://dx.doi.org/10.1186/s13287-019-1431-6.

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Abstract Background In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inconsistent, largely attributed to transplanted MSC difficulties both in engrafting at tissue sites and in retaining their therapeutic functions from suspension formulations. MSC functional components, including cell adhesion and cell–cell junction proteins, and ECM that contribute to essential cellular therapeutic effects, are damaged or removed by proteolytic enzymes us

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