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1

Lee, C. H., A. Khalina, S. H. Lee, and Ming Liu. "A Comprehensive Review on Bast Fibre Retting Process for Optimal Performance in Fibre-Reinforced Polymer Composites." Advances in Materials Science and Engineering 2020 (July 13, 2020): 1–27. http://dx.doi.org/10.1155/2020/6074063.

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Natural fibres are a gift from nature that we still underutilise. They can be classified into several groups, and bast natural fibre reinforcement in polymer composites has the most promising performance, among others. However, numerous factors have reported influences on mechanical properties of the fibre-reinforced composite, including natural fibre retting processes. In this review, bast fibre retting process and the effect of enzymatic retting on the fibre and fibre-reinforced polymer composites have been discussed and reviewed for the latest research studies. All retting methods except chemical and mechanical retting processes are involving secretion of enzymes by bacteria or fungi under controlled (enzymatic retting) or random conditions (water and dew retting). Besides, enzymatic retting is claimed to have more environmentally friendly wastewater products, shorter retting period, and controllable fibre biochemical components under mild incubation conditions. This review comprehensively assesses the enzymatic retting process for producing high-quality bast fibre and will become a reference for future development on bast fibre-reinforced polymer composites.
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2

Majumdar, B., A. R. Saha, S. Sarkar, et al. "An insight into the sequential changes in enzymatic activities during retting of jute (Corchorus spp. L.)." Journal of Environmental Biology 42, no. 3 (2021): 636–43. http://dx.doi.org/10.22438/jeb/42/3/mrn-1604.

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Aim: To study the dynamics of enzymes involved in biochemical process of jute (Corchorus spp.) retting with and without microbial retting consortium. Methodology: Two large scale retting trials were conducted with and without microbial retting consortium in triplicate. The retting water samples were collected every day at 24 hrs interval from both the trials. Polygalcturonase (PG), pectin lyase (PNL) and xylanase activities along with the pH were measured from the collected retting water samples following standard procedure. Fibre quality parameters were also studied from the resultant fibre obtained from both the retting trials. Results: There was a sharp decrease in pH of retting liquor by 1.35 units and that of pectin lyase activity by 97.9 Uml-1 within 24 hrs of inoculation of microbial retting consortium. Thereafter, higher pectin lyase (123.1 Uml-1), polygalacturonase (3.56 Iuml-1) and xylanase (0.818 IUml-1) activities were recorded during middle stage of retting. The enzyme activities were lower and non-significant at last stage of retting (11-14 days). The completion of retting without microbial consortium took longer time due to lower enzymatic activities as compared to microbial consortium mediated retting. Interpretation: The PG, PNL and xylanase enzymes released by the microbial consortium during retting of jute helped in faster biodegradation of pectin and xylan compared to control retting. Hence, the pre retting treatment of jute with microbial consortium is suggested for quick retting.
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3

Kawahara, Yutaka, Tomoyuki Tsuda, Hideaki Minami, Shigenori Nishiuchi, and Rie Endo. "Enzymatic retting of kudzu fibers." Journal of Applied Polymer Science 106, no. 4 (2007): 2759–62. http://dx.doi.org/10.1002/app.25752.

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4

Majumdar, S., A. B. Kundu, S. Dey, and B. L. Ghosh. "Enzymatic retting of jute ribbons." International Biodeterioration 27, no. 3 (1991): 223–35. http://dx.doi.org/10.1016/0265-3036(91)90051-r.

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5

侯, 立波. "Analysis of Bacterial Community Structure in Water Retting and Enzymatic Retting Liquid." Bioprocess 02, no. 03 (2012): 116–22. http://dx.doi.org/10.12677/bp.2012.23019.

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6

De Prez, Jana, Aart Willem Van Vuure, Jan Ivens, Guido Aerts, and Ilse Van de Voorde. "Effect of enzymatic treatment of flax on chemical composition and the extent of fiber separation." BioResources 14, no. 2 (2019): 3012–30. http://dx.doi.org/10.15376/biores.14.2.3012-3030.

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Enzymatic treatment of flax is gaining more interest as a promising alternative for dew retting, which is known for its dependence on weather and climate. Therefore, the effect of enzymatic treatments of flax on the effectiveness of fiber separation from each other and chemical fiber composition was investigated in this study. Chemical composition was determined by a gravimetric method, while ease of separation (in the composites society, the process to obtain natural fibers from the plant is usually defined as extraction) was determined based on the amount of long fibers obtained as well as total time needed to release this fiber fraction, providing necessary insights in the extent to which fibers are loosened from the stem. Flax treatment with pectate lyase and polygalacturonase resulted in purified fibers with a cellulose content of 78 and 79% w/w and promising yield values of 24 and 17%, respectively. Besides these pectinases, xylanase activity also showed high potential for enzymatic retting. Hence, pectate lyase, polygalacturonase, and xylanase are promising enzymes to successfully replace the dew retting process.
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7

Hossain, Mohammad Munir, Shafiquzzaman Siddiquee, and Vijay Kumar. "Critical Factors for Optimum Biodegradation of Bast Fiber’s Gums in Bacterial Retting." Fibers 9, no. 8 (2021): 52. http://dx.doi.org/10.3390/fib9080052.

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Bast fiber plants require a post-harvest process to yield useable natural cellulosic fibers, denoted as retting or degumming. It encompasses the degradation of the cell wall’s non-cellulosic gummy substances (NCGs), facilitating fibers separations, setting the fiber’s quality, and determining downstream usages. Due to the inconvenience of traditional retting practices, bacterial inoculum and enzyme applications for retting gained attention. Therefore, concurrent changes of agroclimatic and socioeconomic conditions, the conventional water retting confront multiple difficulties, bast industries become vulnerable, and bacterial agents mediated augmented bio-retting processes trying to adapt to sustainability. However, this process’s success demands a delicate balance among substrates and retting-related biotic and abiotic factors. These critical factors were coupled to degrade bast fibers NCGs in bacterial retting while holistically disregarded in basic research. In this study, a set of factors were defined that critically regulates the process and requires to be comprehended to achieve optimum retting without failure. This review presents the bacterial strain characteristics, enzyme potentials, specific bast plant cell wall’s structure, compositions, solvents, and interactions relating to the maximum NCGs removal. Among plants, associated factors pectin is the primary biding material that determines the process’s dynamics, while its degree of esterification has a proficient effect through bacterial enzymatic degradation. The accomplished bast plant cell wall’s structure, macerating solvents pH, and temperature greatly influence the bacterial retting process. This article also highlights the remediation process of water retting pollution in a biocompatible manner concerning the bast fiber industry’s endurance.
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8

Ashari, Muhamad Fadzli, Mohd Danial Ibrahim, Awang Husaini, and Azham Zulkharnain. "Accelerated Production of White Pepper Using Integrated Mechanical and Enzymatic Solutions in an Automated Machine." Key Engineering Materials 572 (September 2013): 304–7. http://dx.doi.org/10.4028/www.scientific.net/kem.572.304.

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The enzyme from Aspergillus versicolor A6 under solid state fermentation using agricultural waste as substrates were successfully produced by our group. Laboratory scale enzymatic retting for white pepper production was conducted using crude pectinase enzyme preparation found that decortications of green matured pepper berries were achieved after five days which is comparable to other commercial enzymes. Microbial specification and quality analyses found that retted berries meet the standards set by the International Pepper Community. This study presents a design and development of a machine which integrated mechanical and enzymatic solutions in the decortications of pepper pericarp to improve efficiency the production of white pepper berries as compared to conventional water retting method.
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9

Henriksson, Gunnar, Danny E. Akin, Luanne L. Rigsby, Nirav Patel, and Karl-Erik L. Eriksson. "Influence of Chelating Agents and Mechanical Pretreatment on Enzymatic Retting of Flax." Textile Research Journal 67, no. 11 (1997): 829–36. http://dx.doi.org/10.1177/004051759706701107.

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Adding chelating agents, i.e., oxalic acid and ethylenediamine-tetra-acetic acid (edta), substantially increases the retting effect on flax by the commercial enzyme products Ultrazym and Flaxzyme (Novo Nordisk), as shown by scanning electron microscopy, release of reducing sugars, and the Fried test. Degradation of pectin-rich citrus peel by these enzymes also increases with the addition of oxalic acid and edta, while citric acid has a low or insignificant effect. Oxalic acid at 50 mmol concentration reduces the amount of Flaxzyme required to effectively ret flax stems, according to the Fried test, by a factor of about 50. Retting with Flaxzyme and 50 mmol oxalic acid is completed in approximately half the time at 45°C, compared with that at 22°C. A mechanical pretreatment that crushes flax stems by pulling them over a surface at a 90° angle opens the flax structure and further increases the efficiency of enzymatic retting. These procedures appear to modify both the chemical and structural features of flax, and they reduce the time as well as the amount of enzyme required to ret flax, therefore improving technical efficiency and economic attractiveness at the commercial level.
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10

De Prez, Jana, Aart W. Van Vuure, Jan Ivens, Guido Aerts, and Ilse Van de Voorde. "Flax treatment with strategic enzyme combinations: Effect on fiber fineness and mechanical properties of composites." Journal of Reinforced Plastics and Composites 39, no. 5-6 (2019): 231–45. http://dx.doi.org/10.1177/0731684419884645.

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The application of natural fibers as reinforcement material in composites can make an important contribution toward a more sustainable bio-based economy. To use these natural fibers, they need to be released from the stem. Traditionally, this is realized by dew retting. However, there are some disadvantages to this retting procedure. Due to changing weather conditions, it is quite difficult to guarantee a good and consistent fiber quality. Replacing the traditional retting process by a biocatalytic process may resolve these issues through application of a combination of pectinase and hemicellulase activities. In this context, several strategic pectinase and hemicellulase activities were blended. Flax fiber fineness and mechanical properties of resulting unidirectional fiber–epoxy composites were studied after treatment of flax stems with these strategic enzyme combinations. Characterization of fiber fineness showed a higher percentage of fiber widths smaller than 100 µm after combined treatment with two enzymes (44.7%), and the percentage was even higher after some combinations with three enzymes (51.2%). Assessment of mechanical properties of composites showed no significant differences in tensile or transverse properties for combined enzymatic treatments. Fiber properties were back-calculated from unidirectional flax fiber–epoxy composites and a Young’s modulus of 88 GPa and a tensile strength of 865 MPa were observed. Enzymatic extraction resulted in the achievement of similar results as dew retting concerning mechanical composite performance and fiber fineness.
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11

Zheng, Laijiu, Bing Du, and Weiwei Xue. "Screening and identification ofAcinetobacter juniiforApocynum vernetumL. fiber enzymatic retting." Journal of the Textile Institute 102, no. 8 (2011): 675–80. http://dx.doi.org/10.1080/00405000.2010.514726.

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12

Hossain, Munir, Shafiq Siddiquee, and Vijay Kumar. "Isolation of Alkalophilic Pectinolytic Bacteria and their Bio Retting Effect on Kenaf Fiber Compositions." Alinteri Journal of Agriculture Sciences 36, no. 2 (2021): 156–65. http://dx.doi.org/10.47059/alinteri/v36i2/ajas21129.

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Retting is the most limiting process of high-quality cellulosic kenaf bast fiber production which facilitating the separation of useable fiber from the plants' cell wall matrix. Existing traditional water retting approach confronts ineptitude and eutrophication related complications. Aiming to enhance the kenaf bio-retting process, sixty-seven alkalophilic bacterial colonies were isolated from paddy land soil sediments and kenaf retting water. These isolates were subsequently screened, of that two isolates were selected based on hyper qualitative and quantitative pectinolytic enzymatic measures. 16s rDNA gene sequence analysis revealed that both two strains were closely related to Bacillus pumilus species and designated as KRB56 and KRB22. These strains were applied in augmented non-sterile kenaf tank retting to investigate their kenaf retting efficiency and yielded fiber were analyzed for chemical compositions. Results revealed that, stains KRB56 and KRB22 significantly improve the retting process by degradation of 82.78% and 75.28% non-cellulosic gums, respectively comparing with uninoculated treatment niche (62.12%). Based on un retted raw kenaf fiber maximum fiber bundle weight was reported in MTW with 16.04% material losses, while the SW, and FW treatments showed 24.38%, and 21.03% material losses, respectively. These bacterial treated fiber samples showed thinner, smooth, and cleaner fibers surface morphology by SEM indicates sufficient non cellulosic gums (NCGs) removal comparing with URKF. Moreover, yielded fibers were examined for chemical composition, FTIR, XRD test. Results revealed that compare to un retted and un inoculated kenaf fiber, bacterial treated kenaf fiber increases cellulose portions, and their crystallinity index increases 35.50-41.30 % due to sufficient NCGs removal. This study's findings indicate that isolated alkalophilic bacterial strains KRB56 and KRB22 were effectively to be used as kenaf bio retting agents to produce quality kenaf fiber.
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13

Akin, Danny E., Roy B. Dodd, Warren Perkins, Gunnar Henriksson, and Karl-Erik L. Eriksson. "Spray Enzymatic Retting: A New Method for Processing Flax Fibers." Textile Research Journal 70, no. 6 (2000): 486–94. http://dx.doi.org/10.1177/004051750007000604.

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14

Zhang, Jing, Gunnar Henriksson, and Gunnar Johansson. "Polygalacturonase is the key component in enzymatic retting of flax." Journal of Biotechnology 81, no. 1 (2000): 85–89. http://dx.doi.org/10.1016/s0168-1656(00)00286-8.

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15

Akin, D. E. "UV Absorption Microspectrophotometry and Histochemistry of Flax and Kenaf." Microscopy and Microanalysis 4, S2 (1998): 846–47. http://dx.doi.org/10.1017/s1431927600024351.

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Flax (Linum usitatissimum L.) and kenaf (Hibiscus cannabinus L.) are the sources of fibers used for textiles and other industrial applications. Both flax and kenaf produce fibers in the bast region (Fig. 1, 2) which must be separated from other tissues by retting. Although both flax and kenaf are bast fibers, their properties are vastly different. UV absorption microspectrophotometry and histochemistry elucidate their chemistry and structure related to enzymatic retting.Aromatics such as lignins are produced by plants for protection and strength, but their presence inhibits microbial degradation, which is necessary in retting. Histochemical tests indicated variations in the site and type of aromatics within these two plants (1,2). In flax, acid phloroglucinol but not chlorine-sulfite gave positive reactions occasionally in fiber cell walls in the bast. The other cell walls in the bast did not contain aromatics by these tests, although aromatics occurred in the cuticle.
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16

Barrett, Timothy. "Enzymatic Pretreatment during 15th to 18th Century Papermaking in Europe." BioResources 11, no. 2 (2016): 2964–67. http://dx.doi.org/10.15376/biores.11.2.2964-2967.

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Enzymatic pretreatment of cellulose is generally considered to be a new area of research, but in fact it was a standard step in European pre-industrial papermaking between 1300 and 1800. Specialized handmade papers are routinely prepared in our Center for use in the care and conservation of rare books and manuscripts. Our attempts to replicate some of the early papers have led us to an investigation of retting (or fermentation) of hemp and cotton fiber as a pre-beating step. Results of twenty-two production runs show that increased fermentation time gives increased Canadian Standard Freeness and improved formation quality while permitting a decrease in beating time.
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17

Liu, Ming, Diogo Alexandre Santos Silva, Dinesh Fernando, et al. "Controlled retting of hemp fibres: Effect of hydrothermal pre-treatment and enzymatic retting on the mechanical properties of unidirectional hemp/epoxy composites." Composites Part A: Applied Science and Manufacturing 88 (September 2016): 253–62. http://dx.doi.org/10.1016/j.compositesa.2016.06.003.

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18

Kalisz, Grzegorz, Barbara Gieroba, Olga Chrobak, Magdalena Suchora, Agata L. Starosta, and Anna Sroka-Bartnicka. "Vibrational Spectroscopic Analyses and Imaging of the Early Middle Ages Hemp Bast Fibres Recovered from Lake Sediments." Molecules 26, no. 5 (2021): 1314. http://dx.doi.org/10.3390/molecules26051314.

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Fourier Transform Infrared (FT-IR) spectroscopy and imaging combined with hierarchical cluster analysis (HCA) was applied to analyse biochemical properties of Early Middle Ages hemp (Cannabis sativa L.) bast fibres collected from lake bottom sediment of lake Słone. The examined plant macrofossil material constitutes residues of the hemp retting process that took place in the 7th–8th century. By comparison of three samples: untreated isolated bast fibres, and fibres incubated overnight at 4 and 37 °C, we were able to mimic the retting conditions. Using FT-IR qualitative and semi-quantitative assessment of the primary polysaccharides content, total protein content, and their spatial distribution was performed within the hemp fibres. The concentration of cellulose remained vastly unchanged, while the concentration of lignin and pectin was the highest in the untreated sample. The spatial distributions of compounds were heterogeneous in the untreated and 4 °C-incubated samples, and homogenous in the specimen processed at 37 °C. Interestingly, a higher amide content was detected in the latter sample indicating the highest degree of enzymatic degradation. In this study, we show that the spectroscopic methods allow for a non-destructive evaluation of biochemical composition of plant fibres without preparation, which can be an appropriate approach for studying ancient plant remains.
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19

Ruan, Peiying, Vijaya Raghavan, Jianmin Du, Yvan Gariepy, Darwin Lyew, and Hongyan Yang. "Effect of radio frequency pretreatment on enzymatic retting of flax stems and resulting fibers properties." Industrial Crops and Products 146 (April 2020): 112204. http://dx.doi.org/10.1016/j.indcrop.2020.112204.

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20

Ruan, Peiying, Jianmin Du, Vijaya Raghavan, Darwin Lyew, Yvan Gariepy, and Hongyan Yang. "Microwave pretreated enzymatic retting of flax stems and comparison with the effect of radio frequency pretreatment." Industrial Crops and Products 151 (September 2020): 112312. http://dx.doi.org/10.1016/j.indcrop.2020.112312.

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21

Kazimierczak, Janusz, Justyna Wietecha, Ewa Kopania, Marek Szadkowski, and Lilia Infelise. "Utilization of Post-Maceration Liquid from [i]Spartium junceum[/i] Enzymatic Retting in Biosynthesis of Bacterial Nanocellulose." Fibres and Textiles in Eastern Europe 24, no. 6(120) (2016): 45–50. http://dx.doi.org/10.5604/12303666.1221736.

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22

Tanner, Robert D., Aleš Prokop, and Rakesh K. Bajpai. "Removal of fiber from vines by solid state fermentation/enzymatic degradation: A comparison of flax and kudzu retting." Biotechnology Advances 11, no. 3 (1993): 635–43. http://dx.doi.org/10.1016/0734-9750(93)90032-i.

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23

Himmelsbach, David S., Sadia Khalili, and Danny E. Akin. "The use of FT-IR microspectroscopic mapping to study the effects of enzymatic retting of flax (Linum usitatissimum L) stems." Journal of the Science of Food and Agriculture 82, no. 7 (2002): 685–96. http://dx.doi.org/10.1002/jsfa.1090.

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24

Archibald, Douglas D., and Danny E. Akin. "Use of spectral window preprocessing for selecting near-infrared reflectance wavelengths for determination of the degree of enzymatic retting of intact flax stems." Vibrational Spectroscopy 23, no. 2 (2000): 169–80. http://dx.doi.org/10.1016/s0924-2031(99)00090-9.

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25

Obi, C. N., and U. B. Agu. "Enzymatic Potentials of Microorganisms Associated With Cassava Retting." Journal of Advances in Microbiology, August 9, 2019, 1–9. http://dx.doi.org/10.9734/jamb/2019/v17i430147.

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The enzymatic potentials of microorganisms isolated from retting cassava were evaluated for the purpose of pectinase, cellulase and amylase activities. Cassava tubers of 12 months old were collected from 3 different sources: Ahieke, Ndoru and Umuariga in Abia State. They were hand peeled, cut into cylinders, washed, submerged into water and allowed to ret. After retting a dilution of the retted tubers was inoculated into different media plates: De-Man Rogosa Sharp agar, Sabouraud Dextrose agar, Nutrient agar, Mannitol salt agar and MacConkey agar and incubated at 30°C for 3-5 days. 35 isolates were identified in the retting cassava samples which are bacteria 25(71.43%) and fungi 10(28.57%). The bacterial isolates identified include Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Lactobacillus spp and Proteus spp while fungi isolated were Aspergillus niger, Rhodotorula spp and Saccharomyces cerevisiae. The total viable counts of the isolates increased as the retting hours increased. Lactobacillus spp and Saccharomyces cerevisiae dominated the later stage of the fermentation. Bacillus subtilis has the highest percentage occurrence 8(22.9%) while Rhodotorula spp has the lowest percentage occurrence 2(5.7%). The production of amylase enzyme was recorded with all the isolates with the exception of Staphylococcus aureus. Production of cellulase and pectinase enzymes was selective among the isolates. Results from this study shows that enzymes like amylase, pectinase and cellulase from microorganisms played an important role in retting of cassava tubers.
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26

Ray, Syandan Sinha, Suparna Das, Md Nasim Ali, and Lucina Yeas. "Biochemical characterization and Biolog based identification of efficient Jute retting bacterial isolates from retting water." Journal of Phytology 7 (November 26, 2015). http://dx.doi.org/10.19071/jp.2015.v7.2849.

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Jute is considered as one of the cheapest natural fibre after cotton in terms of its production and uses. Retting is the most important post-harvest operation to yield high quality jute fibre and is solely carried out by various types of retting microorganisms. The present study was undertaken to screen and characterize the efficient retting microbes isolated from retting water based on their enzymatic activity followed by biolog based idenfication of those efficient microbes. These isolates were characterized on the basis of qualitative and quantitative estimation of Pectinolytic, Xylanase and Cellulase activity. Out of 40 isolated strains only 3 were finally identified as efficient jute retting microorganism having high pectinolytic and Xylanase activity coupled with less Cellulase activity. These identified three micro organisms may provide a suitable means to develop a new retting technique especially under water stress condition.
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27

Wong, Li Yin, Wan Zuhainis Saad, Rosfarizan Mohamad, and Paridah Md Tahir. "Efficacy of Aspergillus fumigatus R6 Pectinase in Enzymatic Retting of Kenaf." BioResources 11, no. 4 (2016). http://dx.doi.org/10.15376/biores.11.4.10030-10041.

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28

Kumar, Amit, Mukesh Yadav, and Workinesh Tiruneh. "Debarking, pitch removal and retting: Role of microbes and their enzymes." Physical Sciences Reviews 5, no. 10 (2020). http://dx.doi.org/10.1515/psr-2019-0048.

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AbstractMicrobial enzymes are green and clean alternatives for several processes in the pulp and paper industry. Enzyme treatment decreases the energy requirement and minimizes the wood losses during drum debarking. Lipophilic wood extractives are known as pitch. Pitch deposition adversely affects the pulp quality and increases equipment maintenance and operating costs during paper manufacturing. Several chemical additives have been used to remove pitch deposits. Natural seasoning of wood is used to minimize pitch content in wood, but it has some disadvantages including yield losses and decreased brightness. Controlled seasoning with white-rot fungi or albino strains of sapstain fungi is an effective tool for degradation and removal of wood extractives. Enzymes including lipase, laccase, sterol esterase, and lipooxygenase have also been used to minimize pitch-related problems. Enzymatic retting has been proved an eco-friendly and economical solution for chemical degumming and traditional retting.
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29

Werchefani, Mouna, Catherine Lacoste, Hafedh Belguith, and Chedly Bradai. "Alfa fibers for Cereplast bio-composites reinforcement: Effects of chemical and biological treatments on the mechanical properties." Polymers and Polymer Composites, April 5, 2021, 096739112110060. http://dx.doi.org/10.1177/09673911211006067.

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The present work is a comparative study of the impact of Alfa fiber modifications on the Cereplast composites mechanical behavior. Various treatments have been employed, including mechanical, soda, saltwater-retting, hot-water treatments and enzymatic treatment using xylanase. Chemical and morphological analyses were carried out in order to determine the changes of the biochemical composition and the dimensions of fibers. Cereplast composites reinforced with Alfa fibers were fabricated using a twin-screw extrusion followed by an injection molding technique with a fiber load of 20 wt. %. Resulting materials were assessed by means of tensile, flexural and Charpy impact testing. Scanning Electron Microscopy analysis was carried out to investigate the interfacial properties of the composites. The results have shown a significant enhancement of mechanical strengths and rigidities for the xylanase-treated fiber composites, owing to the increase of cellulose content, the enhancement of defibrillation level and the improvement of matrix-fiber adhesion. The data proved that the technology of enzymes can be used as a powerful and eco-friendly approach to modify fiber surfaces and to increase their potential of reinforcement.
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