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Journal articles on the topic 'Enzymaticly derived extraction'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Petrov, Ivanković Anja, Ana Milivojević, Marija Ćorović, et al. "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives." Chemical and Biological Technologies in Agriculture 10, no. 2023 (2023): 125. https://doi.org/10.1186/s40538-023-00502-8.

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Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by e

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2

Chaudhary, Sonal, Anjali Bhardwaj, and Shalini Juneja. "Innovations and Challenges in Bast Fibre Extraction for Sustainable Development." Advances in Research 26, no. 1 (2025): 257–73. https://doi.org/10.9734/air/2025/v26i11251.

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Bast fibres, derived from the inner bark of plants such as jute, flax, hemp, and kenaf, play a significant role in various industries, including textiles, composites, and bioplastics. The extraction of these fibres is a crucial step that affects their quality and sustainability. However, traditional extraction methods, such as water and dew retting, while cost-effective, pose environmental challenges and yield inconsistent fibre quality. More advanced methods, including enzymatic, biological, and mechanical extractions, offer promising alternatives but come with their own set of challenges, su

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3

Le Strat, Yoran, Margaux Mandin, Nicolas Ruiz, et al. "Quantification of Xylanolytic and Cellulolytic Activities of Fungal Strains Isolated from Palmaria palmata to Enhance R-Phycoerythrin Extraction of Palmaria palmata: From Seaweed to Seaweed." Marine Drugs 21, no. 7 (2023): 393. http://dx.doi.org/10.3390/md21070393.

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R-phycoerythrin (R-PE) can be enzymatically extracted from red seaweeds such as Palmaria palmata. This pigment has numerous applications and is notably known as an antioxidant, antitumoral or anti-inflammatory agent. Enzymes secreted by P. palmata associated fungal strains were assumed to be efficient and adapted for R-PE extraction from this macroalga. The aim of the present study was to quantify both xylanolytic and cellulolytic activities of enzymatic extracts obtained from six Palmaria palmata derived fungal strains. Degradation of P. palmata biomass by fungal enzymatic extracts was also i

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4

Kim, Naeun, Jihyeon Yu, Yoonjeong Kim, Eunji Park, Youngmin Choi, and Younghwa Kim. "Enzymatic hydrolysis for biotin extraction from animal-derived foods." Food Science and Preservation 31, no. 5 (2024): 837–45. http://dx.doi.org/10.11002/fsp.2024.31.5.837.

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Biotin exists as free or covalently bound to protein through a lysine residue. Therefore, the extraction of free biotin form is difficult in foodstuffs. Thus, the aim of this study was to investigate the extraction method of biotin in animal-derived foods by enzymatic hydrolysis (pepsin) using autoclave extraction or sonication extraction. Biotin contents of animal origin foods such as meat (beef, foreshank), fish and shells (mackerel), and egg (yolk, raw) were significantly higher in the autoclave extraction method with pepsin treatment. Therefore, these methods were selected for further appl

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Gordalina, Mariana, Helena M. Pinheiro, Marília Mateus, M. Manuela R. da Fonseca, and M. Teresa Cesário. "Macroalgae as Protein Sources—A Review on Protein Bioactivity, Extraction, Purification and Characterization." Applied Sciences 11, no. 17 (2021): 7969. http://dx.doi.org/10.3390/app11177969.

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The increased demand for protein sources combined with a decrease in the available land and water resources have led to a growing interest in macroalgae as alternative protein sources. This review focuses on strategies for macroalgae protein extraction, enrichment and characterization. To date, the protein extraction methods applied to algae include enzymatic hydrolysis, physical processes and chemical extraction. Novel methods, such as pulsed electric field, microwave-assisted, pressurized liquid and supercritical fluid extractions, and the application of smart solvents are discussed. An over

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6

Badfar, Narjes, Ali Jafarpour, Federico Casanova, et al. "Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste." Marine Drugs 23, no. 3 (2025): 122. https://doi.org/10.3390/md23030122.

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This study explored how combining supercritical fluid extraction (SFE) and enzymatic hydrolysis influences the structure and functionality of peptides recovered from filter-pressed shrimp waste. Freeze-dried press cake (PC) was defatted via SFE and hydrolyzed using Alcalase (ALC) and trypsin (TRYP). ALC-treated PC achieved the highest protein recovery (63.49%), extraction yield (24.73%), and hydrolysis degree (18.10%) (p < 0.05). SFE-treated hydrolysates showed higher zeta potential (−47.23 to −49.93 mV) than non-SFE samples (−25.15 to −38.62 mV) but had larger droplet sizes, indicating low

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7

Ha, Si-Young, Hyeon-Cheol Kim, and Jae-Kyung Yang. "Enzymatic Hydrolysis-Derived Water-Soluble Carbohydrates from Cacalia firma: Evaluation of Antioxidant Properties." Foods 14, no. 8 (2025): 1326. https://doi.org/10.3390/foods14081326.

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This research focused on producing water-soluble carbohydrates extracts from the leaves of the wild plant Cacalia firma using commercial enzymatic processes. Different enzymes and conditions were applied to the leaves to determine the optimal method for extracting carbohydrates. Enzymes used were Cellic CTec3 HS, Celluclast 1.5 L, Viscozyme L, Pectinex ultraSP-L, and Amylase AG. Pectinase, cellulase, and other enzymes are isolated from yeast, bacteria, or some higher plants and are commonly used to break down pectin, which is the cell wall or intercellular connective tissue in plant tissues, t

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8

Mostashari, Parisa, Krystian Marszałek, Aynura Aliyeva, and Amin Mousavi Khaneghah. "The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg." Molecules 28, no. 6 (2023): 2658. http://dx.doi.org/10.3390/molecules28062658.

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This review article discusses advanced extraction methods to enhance the functionality of egg-derived peptides while reducing their allergenicity. While eggs are considered a nutrient-dense food, some proteins can cause allergic reactions in susceptible individuals. Therefore, various methods have been developed to reduce the allergenicity of egg-derived proteins, such as enzymatic hydrolysis, heat treatment, and glycosylation. In addition to reducing allergenicity, advanced extraction methods can enhance the functionality of egg-derived peptides. Techniques such as membrane separation, chroma

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9

Velyamov, Massimzhan, Zhumatay Urazbayev, Shukhrat Velyamov, Turar Bakytzhan, and Aelina Abitbekova. "Development of technology for producing protein hydrolysates from leguminous plants (peas) for sports nutrition." Scifood 19 (January 1, 2025): 79–95. https://doi.org/10.5219/scifood.6.

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In line with emerging trends, the sports nutrition industry actively seeks cost-effective and health-promoting protein ingredients to replace animal-derived products and transition toward more sustainable practices. Protein concentrates derived from legumes, including peas, have attracted consumer interest due to their rich amino acid profiles and favourable functional properties. Our research aimed to develop and optimise an enzymatic technology for producing protein hydrolysates from locally adapted pea varieties in Kazakhstan. Based on the research findings, optimal technological conditions

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Kruschitz, Andreas, and Bernd Nidetzky. "Reactive extraction of fructose for efficient separation of sucrose-derived glucosides produced by enzymatic glycosylation." Green Chemistry 22, no. 15 (2020): 4985–94. http://dx.doi.org/10.1039/d0gc01408g.

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Jirarat, Wilasinee, Tanyawat Kaewsalud, Kamon Yakul, Pornchai Rachtanapun, and Thanongsak Chaiyaso. "Sustainable Valorization of Coffee Silverskin: Extraction of Phenolic Compounds and Proteins for Enzymatic Production of Bioactive Peptides." Foods 13, no. 8 (2024): 1230. http://dx.doi.org/10.3390/foods13081230.

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Coffee silverskin (CS), a by-product of the coffee roasting process, has high protein content (16.2−19.0%, w/w), making it a potential source for plant protein and bioactive peptide production. This study aims to develop innovative extraction methods for phenolic compounds and proteins from CS. The conditions for hydrothermal (HT) extraction of phenolic compounds from CS were optimized by varying CS loading (2.5−10%, w/v), temperature (110−130 °C), and time (5−30 min) using a one-factor-at-a-time (OFAT) approach. The highest TPC of 55.59 ± 0.12 µmole GAE/g CS was achieved at 5.0% (w/v) CS load

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Lampakis, Dimitrios, Prodromos Skenderidis, and Stefanos Leontopoulos. "Technologies and Extraction Methods of Polyphenolic Compounds Derived from Pomegranate (Punica granatum) Peels. A Mini Review." Processes 9, no. 2 (2021): 236. http://dx.doi.org/10.3390/pr9020236.

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The interest in using plant by-product extracts as functional ingredients is continuously rising due to environmental and financial prospects. The development of new technologies has led to the achievement of aqueous extracts with high bioactivity that is preferable due to organic solvents nonuse. Recently, widely applied and emerging technologies, such as Simple Stirring, Pressure-Applied Extraction, Enzymatic Extraction, Ultrasound-Assisted Extraction, Pulsed Electric Fields, High Hydrostatic Pressure, Ohmic Heating, Microwave Assistant Extraction and the use of “green” solvents such as the

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13

Lyon, Frank L., and Judith E. Domer. "Chemical and enzymatic variation in the cell walls of pathogenic Candida species." Canadian Journal of Microbiology 31, no. 7 (1985): 590–97. http://dx.doi.org/10.1139/m85-112.

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Cell walls, isolated from seven pathogenic species of Candida, were lipid extracted and fractionated by treatment with ethylenediamine or enzymatically hydrolyzed using chitinase and laminarinase. Two different chitinase preparations were used, one from Streptomyces sp. which had some β-1,3-glucanase activity, and another from Serratia marcescens which did not have glucanase activity. Laminarinase was a commercial preparation. The monosaccharide constituents of whole cell walls and the fractions derived from them were determined qualitatively and quantitatively by gas–liquid chromatography of

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14

Turner, Charlotta, Jerry W. King, and Thomas McKeon. "Selected Uses of Enzymes with Critical Fluids in Analytical Chemistry." Journal of AOAC INTERNATIONAL 87, no. 4 (2004): 797–810. http://dx.doi.org/10.1093/jaoac/87.4.797.

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Abstract The use of enzymes coupled with supercritical fluid (SF)-based analytical techniques, such as supercritical fluid extraction (SFE), provides a safer environment platform for the analytical chemist and reduces the use of organic solvents. Incorporation of such techniques not only reduces the use of solvent in analytical laboratories, but it can also lead to overall method simplification and time savings. In this review, some of the fundamental aspects of using enzymes in the presence of SF media are discussed, particularly the influence of extraction (reaction) pressure, temperature, a

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15

Yan, Pei Sheng, Li Ya Ma, and Li Xin Cao. "Optimization of Enzymatic Hydrolytic Extraction Conditions for High Yield Polysaccharide Manufacturing from Liquid Fermented Materials of Hypsizigus Marmoreus." Applied Mechanics and Materials 145 (December 2011): 154–58. http://dx.doi.org/10.4028/www.scientific.net/amm.145.154.

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Polysaccharides extracted from fruiting body and mycelia of higher fungi have been found to show various biological activities. Traditionally, these polysaccharides are manufactured by the hot water extraction method. Low polysaccharide yield is the disadvantage of this method. This paper reports the optimal conditions to manufacture polysaccharide with high-yield from Hypsizigus marmoreus mycelia using enzymatic hydrolytic extraction method. Neutral protease was selected as the most cost efficient among six enzymes. The uniform design was further conducted to optimize the enzymatic hydrolytic

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16

Chauhan, Ajeet Singh, Chiu-Wen Chen, Reeta Rani Singhania, et al. "Valorizations of Marigold Waste for High-Value Products and Their Industrial Importance: A Comprehensive Review." Resources 11, no. 10 (2022): 91. http://dx.doi.org/10.3390/resources11100091.

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The municipal authorities in developing nations face serious challenges in marigold flower garbage management. The primary issue is that they never are reused after prayers. Flower waste of Tagetes erecta, T. patula, and Calendula officinalis L. are commonly used for carotenoid and flavonoid extractions and, subsequently, used for incense stick and biogas production. Marigold plants are also used for phytoremediation during their growth stage. The lutein industry is booming due to its increasing market demand, expected to reach ~2121.2 billion tons by 2022, where marigolds are a major contribu

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17

Chavan, Shivanand, and Ashwin Gaikwad. "Extraction and recovery of lignin derived phenolic inhibitors to enhance enzymatic glucose production." Biomass and Bioenergy 144 (January 2021): 105897. http://dx.doi.org/10.1016/j.biombioe.2020.105897.

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18

Masoumifeshani, Baran, Abdolmohammad Abedian Kenari, Ignacio Sottorff, Max Crüsemann, and Jamshid Amiri Moghaddam. "Identification and Evaluation of Antioxidant and Anti-Aging Peptide Fractions from Enzymatically Hydrolyzed Proteins of Spirulina platensis and Chlorella vulgaris." Marine Drugs 23, no. 4 (2025): 162. https://doi.org/10.3390/md23040162.

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Microalgae are a promising source of bioactive compounds, particularly proteins and peptides, with potential applications in skin health and the cosmetic industry. This study investigated the antioxidant and anti-aging properties of peptide fractions derived from Spirulina platensis and Chlorella vulgaris. Both microalgae were cultivated, and their proteins were subsequently extracted, enzymatically hydrolyzed with alcalase, and fractionated through ultrafiltration. Alkaline extraction yielded 82% protein from S. platensis and 72% from C. vulgaris. Enzymatic hydrolysis predominantly yielded &l

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19

Mohamad, S. A., and Z. T. Sedrah. "Studying the Effectiveness of Bromelain Enzyme Extracted from Pineapple Against Anti-Inflammatory and Anti-Cancer Cells." IOP Conference Series: Earth and Environmental Science 1252, no. 1 (2023): 012158. http://dx.doi.org/10.1088/1755-1315/1252/1/012158.

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Abstract The experiment was carried out in the laboratories of the College of Agricultural Engineering Sciences, University of Baghdad, Bromelain enzyme was extracted from pineapple fruit crown and pineapple peel. An extraction solution was used with different mixing ratios from 1:1 to 5:1. The mixing ratio of 2:1 was the best extraction ratio, as it reached the highest enzymatic activity among the other mixing ratios, which was 33 units. The enzymatic activity of the crown extract was higher than the enzymatic activity of the peel extract, reaching 9.8 units/ml and 1.1 units/ml, respectively,

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Shahidi, Fereidoon, and Abu Saeid. "Bioactivity of Marine-Derived Peptides and Proteins: A Review." Marine Drugs 23, no. 4 (2025): 157. https://doi.org/10.3390/md23040157.

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The marine environment, covering over 70% of the Earth's surface, serves as a reservoir of bioactive molecules, including peptides and proteins. Due to the unique and often extreme marine conditions, these molecules exhibit distinctive structural features and diverse functional properties, making them promising candidates for therapeutic applications. Marine-derived bioactive peptides, typically consisting of 3 to 40 amino acid residues—though most commonly, 2 to 20—are obtained from parent proteins through chemical or enzymatic hydrolysis, microbial fermentation, or gastrointestinal digestion

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21

Kyriakou, Sotiris, Dimitrios T. Trafalis, Maria V. Deligiorgi, Rodrigo Franco, Aglaia Pappa, and Mihalis I. Panayiotidis. "Assessment of Methodological Pipelines for the Determination of Isothiocyanates Derived from Natural Sources." Antioxidants 11, no. 4 (2022): 642. http://dx.doi.org/10.3390/antiox11040642.

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Isothiocyanates are biologically active secondary metabolites liberated via enzymatic hydrolysis of their sulfur enriched precursors, glucosinolates, upon tissue plant disruption. The importance of this class of compounds lies in their capacity to induce anti-cancer, anti-microbial, anti-inflammatory, neuroprotective, and other bioactive properties. As such, their isolation from natural sources is of utmost importance. In this review article, an extensive examination of the various parameters (hydrolysis, extraction, and quantification) affecting the isolation of isothiocyanates from naturally

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22

Cheong, Kit-Leong, Amanullah Sabir, Min Wang, Saiyi Zhong, and Karsoon Tan. "Advancements in the Extraction, Characterization, and Bioactive Potential of Laminaran: A Review." Foods 14, no. 10 (2025): 1683. https://doi.org/10.3390/foods14101683.

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Laminaran, a bioactive β-glucan derived from brown algae, has garnered significant attention due to its diverse pharmacological properties, including antioxidant, immunomodulatory, and mucosal protective effects. Despite promising research highlighting its potential applications in functional foods, nutraceuticals, and pharmaceuticals, the commercial utilization of laminaran remains limited, primarily due to challenges in extraction efficiency, structural complexity, and a lack of standardized methodologies. This review critically examines recent advancements in the extraction, purification, s

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Ortizo, Rhessa Grace Guanga, Vishal Sharma, Mei-Ling Tsai, et al. "Extraction of Novel Bioactive Peptides from Fish Protein Hydrolysates by Enzymatic Reactions." Applied Sciences 13, no. 9 (2023): 5768. http://dx.doi.org/10.3390/app13095768.

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Bioactive peptides derived from fish the byproduct protein hydrolysate have wide potential as functional food ingredients. The preparation of bioactive peptides is commonly achieved via enzymatic hydrolysis; this is the most preferred method because it has high specificity, fewer residual organic solvents in the product, and it is usually carried out in mild conditions. The use of various enzymes such as proteases is widely practiced in the industry, yet there are various limitations as it is of high cost and there is a limited availability of food-grade enzymes in the market. Moreover, high-t

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Siriwat, Warin, Sunisa Ungwiwatkul, Kridsada Unban, et al. "Extraction, Enzymatic Modification, and Anti-Cancer Potential of an Alternative Plant-Based Protein from Wolffia globosa." Foods 12, no. 20 (2023): 3815. http://dx.doi.org/10.3390/foods12203815.

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The global plant-based protein demand is rapidly expanding in line with the increase in the world’s population. In this study, ultrasonic-assisted extraction (UAE) was applied to extract protein from Wolffia globosa as an alternative source. Enzymatic hydrolysis was used to modify the protein properties for extended use as a functional ingredient. The successful optimal conditions for protein extraction included a liquid to solid ratio of 30 mL/g, 25 min of extraction time, and a 78% sonication amplitude, providing a higher protein extraction yield than alkaline extraction by about 2.17-fold.

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Chen, Yang, Jinying Shi, Huamai Qiu, et al. "Characterization of Three Polysaccharide-Based Hydrogels Derived from Laminaria japonica and Their Hemostatic Properties." Marine Drugs 22, no. 4 (2024): 188. http://dx.doi.org/10.3390/md22040188.

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Three Laminaria japonica polysaccharides (LJPs) extracted via water extraction (LJP-W), acid extraction (LJP-A), and enzymatic extraction (LJP-E) were used as raw materials to be cross-linked with chitosan and polyvinyl alcohol to prepare hydrogels. Compared with conventional hydrogel systems, all three types of LJP-based polysaccharide hydrogels exhibited better swelling properties (14 times their original weight) and the absorption ability of simulated body fluid (first 2 h: 6–10%). They also demonstrated better rigidity and mechanical strength. Young’s modulus of LJP-E was 4 times that of t

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Li, Tao, Wenhua Ji, Hongjing Dong, et al. "A Comprehensive Review on the Isolation, Bioactivities, and Structure–Activity Relationship of Hawthorn Pectin and Its Derived Oligosaccharides." Foods 13, no. 17 (2024): 2750. http://dx.doi.org/10.3390/foods13172750.

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Hawthorn (Crataegus pinnatifida Bunge) has been highlighted as an excellent source of a variety of bioactive polymers, which has attracted increasing research interest. Pectin, as a kind of soluble dietary fiber in hawthorn, is mainly extracted by hot water extraction and ultrasonic or enzymatic hydrolysis and is then extensively used in food, pharmaceutical, and nutraceutical industries. Numerous studies have shown that hawthorn pectin and its derived oligosaccharides exhibit a wide range of biological activities, such as antioxidant activity, hypolipidemic and cholesterol-reducing effects, a

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Singh Rawat, Gandharv, Satish Mohabe, Bindu Nahar, Neha Paliwal, and Jitendra Malviya. "Exploring Protease Enzymes from Extremophiles Novel Solutions for Sustainable Leather Processing." International Journal of Current Microbiology and Applied Sciences 13, no. 7 (2024): 281–310. http://dx.doi.org/10.20546/ijcmas.2024.1307.028.

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This review article highlights the industrial relevance of extremophile proteases and examines methods for isolating and characterizing them. Microorganisms known as extremophiles, which flourish in harsh conditions, have special enzymatic properties that may have important biotechnological uses. The methodical procedure includes gathering samples from extreme environments, isolating extremophiles, enrichment culture, and screening for protease activity. In order to predict the structure and function of proteins, promising strains go through gene sequencing, PCR amplification, genomic DNA extr

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Fguiri, Imen, Amel Sboui, Nahed Yousfi, et al. "Usefulness of <i>Moringa oleifera</i> seed extract as Coagulant for the production of fresh camel cheese." Italian Journal of Food Science 36, no. 3 (2024): 243–53. http://dx.doi.org/10.15586/ijfs.v36i3.2517.

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The current study aimed to enhance the coagulation properties of camel milk using enzymatic extracts derived from Moringa oleifera seeds. Experiments were conducted to evaluate the clotting activities of Moringa extract on camel milk and compare them with cow milk. Our results regarding the characterisation of the enzymatic extract showed an extraction yield of 54.3±1.8%. The optimum coagulation conditions were determined to be pH 5 and a temperature of 55°C. In addition, the enzymes exhibited substantial clotting activity (2.54 RU) on both camel and cow milk. The cheese samples showed signifi

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Gómez, Belén, Remedios Yáñez, Juan Carlos Parajó, and José Luis Alonso. "Production of pectin-derived oligosaccharides from lemon peels by extraction, enzymatic hydrolysis and membrane filtration." Journal of Chemical Technology & Biotechnology 91, no. 1 (2014): 234–47. http://dx.doi.org/10.1002/jctb.4569.

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Taher, Hanifa, Sulaiman Al-Zuhair, Ali H. Al-Marzouqi, Yousef Haik, and Mohammed M. Farid. "A Review of Enzymatic Transesterification of Microalgal Oil-Based Biodiesel Using Supercritical Technology." Enzyme Research 2011 (September 11, 2011): 1–25. http://dx.doi.org/10.4061/2011/468292.

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Biodiesel is considered a promising replacement to petroleum-derived diesel. Using oils extracted from agricultural crops competes with their use as food and cannot realistically satisfy the global demand of diesel-fuel requirements. On the other hand, microalgae, which have a much higher oil yield per hectare, compared to oil crops, appear to be a source that has the potential to completely replace fossil diesel. Microalgae oil extraction is a major step in the overall biodiesel production process. Recently, supercritical carbon dioxide (SC-CO2) has been proposed to replace conventional solve

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Sedlar, Tea, Igor Pasković, Danka Dragojlović, et al. "Enzyme-Assisted Extraction of Proteins from Cauliflower and Broccoli Waste Leaves." Horticulturae 11, no. 2 (2025): 148. https://doi.org/10.3390/horticulturae11020148.

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This research presents a novel approach to protein extraction from cauliflower (CL) and broccoli (BL) waste leaves by using enzymatic pretreatment, demonstrating its effectiveness on protein yield. Enzymatic pretreatment (pH 4.5, 10 h, t = 35 °C), was performed using commercial enzyme preparations, Viscozyme® L and Vinozyme®, in three different enzyme-to-substrate ratios E/S (0.2%, 2.5%, and 4.8%) of each enzyme. Leaf proteins (LPs) were obtained with alkaline extraction at pH 10–11 and their isoelectric precipitation at pH 4, following the control sample (extraction without enzymes). Protein

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Banik, Swarnali, Shrutidhara Biswas, and Srabani Karmakar. "Extraction, purification, and activity of protease from the leaves of Moringa oleifera." F1000Research 7 (July 30, 2018): 1151. http://dx.doi.org/10.12688/f1000research.15642.1.

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Background: Proteases cleave proteins, thereby providing essential amino acids for protein synthesis, and degrade misfolded and damaged proteins to maintain homeostasis. Proteases also serve as signaling molecules, therapeutic agents and find wide applications in biotechnology and pharmaceutical industry. Plant-derived proteases are suitable for many biomedical applications due to their easy availability and activity over a wide range of pH, temperature, and substrates. Moringa oleifera Lam (Moringaceae) is a very common food plant with medicinal property and geographically distributed in trop

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Schiavi, Daniele, Riccardo Ronchetti, Veronica Di Lorenzo, et al. "Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato." Plants 12, no. 4 (2023): 939. http://dx.doi.org/10.3390/plants12040939.

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Nanotechnology is rapidly gaining ground in crop protection, with the growing quest for sustainable nanopesticides and nanocarriers for plant pathogen management. Among them, cellulose nanocrystals (CNC) are emerging as innovative agrofood-waste-derived antimicrobial materials. In this work, new chemical and enzymatic CNC extraction methods from tomato harvest residues were evaluated. The obtained nanomaterials were characterized and tested for their antimicrobial properties on Pseudomonas syringae pv. tomato (Pto), the causal agent of bacterial speck disease on tomato. Both protocols were eff

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Ahmmed, Mirja Kaizer, Mayssa Hachem, Fatema Ahmmed, et al. "Marine Fish-Derived Lysophosphatidylcholine: Properties, Extraction, Quantification, and Brain Health Application." Molecules 28, no. 7 (2023): 3088. http://dx.doi.org/10.3390/molecules28073088.

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Long-chain omega-3 fatty acids esterified in lysophosphatidylcholine (LPC-omega-3) are the most bioavailable omega-3 fatty acid form and are considered important for brain health. Lysophosphatidylcholine is a hydrolyzed phospholipid that is generated from the action of either phospholipase PLA1 or PLA2. There are two types of LPC; 1-LPC (where the omega-3 fatty acid at the sn-2 position is acylated) and 2-LPC (where the omega-3 fatty acid at the sn-1 position is acylated). The 2-LPC type is more highly bioavailable to the brain than the 1-LPC type. Given the biological and health aspects of LP

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Mateos-Aparicio, Inmaculada, Elena Pérez-López, and Pilar Rupérez. "Valorisation Approach for the Soybean By-Product Okara Using High Hydrostatic Pressure." Current Nutrition & Food Science 15, no. 6 (2019): 548–50. http://dx.doi.org/10.2174/1573401314666180516092837.

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Okara is a perishable, cheap and abundant by-product derived from soybean after extracting the soluble fraction for tofu or soybean drink, mainly known as soymilk, production. Nowadays, Okara is mostly discarded: landfill and incineration, but a useful alternative for valorisation would be to use it as a valuable source of dietary fibre. However, it presents low soluble dietary fibre (SDF) content responsible for prebiotic and anti-carcinogenic effects, so an easy industrial transformation to maximize its SDF content would be most interesting for this purpose. Different approaches can be used

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Snyman, Carla, Julie Mekoue Nguela, Nathalie Sieczkowski, Matteo Marangon, and Benoit Divol. "Optimised Extraction and Preliminary Characterisation of Mannoproteins from Non-Saccharomyces Wine Yeasts." Foods 10, no. 5 (2021): 924. http://dx.doi.org/10.3390/foods10050924.

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The exogenous application of yeast-derived mannoproteins presents many opportunities for the improvement of wine technological and oenological properties. Their isolation from the cell wall of Saccharomycescerevisiae has been well studied. However, investigations into the efficiency of extraction methods from non-Saccharomyces yeasts are necessary to explore the heterogeneity in structure and composition that varies between yeast species, which may influence wine properties such as clarity and mouthfeel. In this study, nine yeast strains were screened for cell wall mannoprotein content using f

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Amid, Mehrnoush, Mohd Yazid Abdul Manap, and Norkhanani Zohdi. "Optimization of Processing Parameters for Extraction of Amylase Enzyme from Dragon (Hylocereus polyrhizus) Peel Using Response Surface Methodology." Scientific World Journal 2014 (2014): 1–12. http://dx.doi.org/10.1155/2014/640949.

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The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1 : 2 to 1 : 6, w/w), temperature (−18°C to 25°), mixing time (60 to 180 seconds), and the pH of the buffer (2.0 to 8.0). The results indicate that the enzyme extraction conditions exhibited the least significant (P<0.05) effect on temperature stability. Conversely, the extraction conditions had the most significant (P<0.05) effect on the specific acti

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Andri Cahyo Kumoro, Andri Cahyo Kumoro, Shinta Mariana Shinta Mariana, Tri Hanly Maurice Tri Hanly Maurice, and Jefri Pandu Hidayat Jefri Pandu Hidayat. "Pectin Derived from Hydrolysis of Ripe Kepok Kuning Banana Peel Powder Employing Crude Pectinases Produced by Aspergillus niger." Sains Malaysiana 51, no. 7 (2022): 2047–59. http://dx.doi.org/10.17576/jsm-2022-5107-09.

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Banana fruits consumption generates about 35% weight of peel waste containing approximately 10.61 to 24 w/w% of pectin. Hence, improper banana peel waste management may induce various environmental and health issues. The objectives of this work were to study the effect of substrate concentration, pH, temperature, and duration on the yield of pectin extracted from enzymatic hydrolysis of banana peel powder. In this work, the crude enzymes were obtained via submerged fermentation of Kepok Kuning banana peel powder utilising Aspergillus niger and directly used without prior purification. Pectin e

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Luterbacher, Jeremy, Jacqueline Rand, Alonso David Martin та ін. "Nonenzymatic sugar production from biomass using biomass-derived γ-valerolactone". SCIENCE 343, № 6168 (2014): 277–80. https://doi.org/10.1126/science.12467.

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Widespread production of biomass-derived fuels and chemicals will require cost-effective processes for breaking down cellulose and hemicellulose into their constituent sugars. Here, we report laboratory-scale production of soluble carbohydrates from corn stover, hardwood, and softwood at high yields (70 to 90%) in a solvent mixture of biomass-derived γ-valerolactone (GVL), water, and dilute acid (0.05 weight percent H<sub>2</sub>SO<sub>4</sub>). GVL promotes thermocatalytic saccharification through complete solubilization of the biomass, including the lignin fraction. The carbohydrates c

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Kim, Tae Hoon, Hyun Kwak, Tae Hyun Kim, and Kyeong Keun Oh. "Extraction Behaviors of Lignin and Hemicellulose-Derived Sugars During Organosolv Fractionation of Agricultural Residues Using a Bench-Scale Ball Milling Reactor." Energies 13, no. 2 (2020): 352. http://dx.doi.org/10.3390/en13020352.

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Ethanol organosolv fractionation combined with ball milling was conducted on three major agricultural residues: Rice husk (RH), rice straw (RS), and barley straw (BS). The highest lignin extraction yields of RH, RS, and BS were 55.2%, 53.1%, and 59.4% and the purity of lignin recovered was 99.5% for RH and RS, and 96.8% for BS, with similar chemical characteristics, i.e., low molecular weight distributions (1453–1817 g/mol) and poly dispersity index (1.15–1.28). However, considering the simultaneous production of hemicellulose-derived sugars, distinctive fractionation behaviors were shown for

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Nikolic, Milos, and Ljiljana Mojovic. "Characterization and degradation of pectin derived from Budimka apple." Journal of the Serbian Chemical Society 73, no. 2 (2008): 157–67. http://dx.doi.org/10.2298/jsc0802157n.

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The characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG) and pectin lyase (PL) from Aspergillus niger and then fractionated by ion-exchange column chromatography on Dowex 1X-8 (200-400 mesh). Saturated oligo?galacturonic acids, obtained by controlled hydrolysis with PG, were efficiently separated by elution with a gradient of Na acetate buffer (pH 6.0), wh

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Cattier, Bettina, Rina Guignard, Israël Martel, et al. "Bulge-Derived Epithelial Cells Isolated from Human Hair Follicles Using Enzymatic Digestion or Explants Result in Comparable Tissue-Engineered Skin." International Journal of Molecular Sciences 26, no. 5 (2025): 1852. https://doi.org/10.3390/ijms26051852.

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Hair follicle stem cells, located in the bulge region of the outer root sheath, are multipotent epithelial stem cells capable of differentiating into epidermal, sebaceous gland, and hair shaft cells. Efficient culturing of these cells is crucial for advancements in dermatology, regenerative medicine, and skin model development. This investigation aimed to develop a protocol for isolating enriched bulge-derived epithelial cells from scalp specimens to produce tissue-engineered substitutes. The epithelium, including hair follicles, was separated from the dermis using thermolysin, followed by mic

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Franca-Oliveira, Giselle, Tiziana Fornari, and Blanca Hernández-Ledesma. "A Review on the Extraction and Processing of Natural Source-Derived Proteins through Eco-Innovative Approaches." Processes 9, no. 9 (2021): 1626. http://dx.doi.org/10.3390/pr9091626.

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In addition to their nutritional and physiological role, proteins are recognized as the major compounds responsible for the rheological properties of food products and their stability during manufacture and storage. Furthermore, proteins have been shown to be source of bioactive peptides able to exert beneficial effects on human health. In recent years, scholarly interest has focused on the incorporation of high-quality proteins into the diet. This fact, together with the new trends of consumers directed to avoid the intake of animal proteins, has boosted the search for novel and sustainable p

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Ramos-Zambrano, Emilia, Pedro Herrera-Serrano, Jorge García-Dávila, Gabriel Ríos-Cortés, Antonio Ruperto Jiménez-Aparicio, and Alma Leticia Martínez-Ayala. "Cochineal Waxy Residues as Source of Policosanol: Chemical Hydrolysis and Enzymatic Transesterification." Journal of Chemistry 2019 (February 10, 2019): 1–10. http://dx.doi.org/10.1155/2019/4547378.

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The aim of this study was to obtain and characterise the long-chain alcohols present in policosanol derived from waste from the production of carminic acid, a natural colouring agent widely used in the food industry. The effectiveness of different methods designed for extraction of policosanol from waxy waste was investigated and its content and composition was determined. Triacontanol was the main component in policosanol produced by chemical processes, and it yields up to 13% by alkaline hydrolysis in water and chloroform extraction. Regarding enzymatic transesterification, policosanol was o

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GANAN, M., A. V. CARRASCOSA, S. de PASCUAL-TERESA, and A. J. MARTINEZ-RODRIGUEZ. "Inhibition by Yeast-Derived Mannoproteins of Adherence to and Invasion of Caco-2 Cells by Campylobacter jejuni." Journal of Food Protection 72, no. 1 (2009): 55–59. http://dx.doi.org/10.4315/0362-028x-72.1.55.

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The main objective of the present work was to study the influence of yeast-derived mannoproteins on the adherence to and invasion of Caco-2 cells by Campylobacter jejuni. Mannoprotein fractions were prepared by enzymatic and thermal extraction methods. The method used to prepare the mannoprotein extracts influenced their composition and determined the efficacy of the extract against C. jejuni adherence and/or invasion. The availability of mannose in the mannoprotein fraction seemed to be important for inhibiting effective adherence and invasion of Caco-2-cells by C. jejuni, although protein mo

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Melkonian, K. I., T. V. Rusinova, E. S. Zaporozhskaya-Abramova, et al. "Comprehensive Evaluation of Decellularized Dental Pulp as a New Biomaterial for Regeneration of the Pulp-Dentin Complex." Innovative Medicine of Kuban, no. 4 (November 15, 2024): 93–100. http://dx.doi.org/10.35401/2541-9897-2024-9-4-93-100.

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Objective: To develop a detergent-enzymatic method and evaluate the quality of a decellularized pulp scaffold for regenerative endodontics.Materials and methods: Biomaterial and mesenchymal stem cells (MSCs) were derived from dental pulp that was obtained following third molar extraction indicated for orthodontic reasons in patients aged 14-18 years. The detergent-enzymatic method enabled to obtain a decellularized scaffold from pulp samples. The proliferative activity and viability of dental pulp-derived MSCs were assessed using trypan blue staining and XTT assay. To assess tissue response, W

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De Rinaldis, Gianluca, Antonella Leone, Stefania De Domenico, et al. "Biochemical Characterization of Cassiopea andromeda (Forsskål, 1775), Another Red Sea Jellyfish in the Western Mediterranean Sea." Marine Drugs 19, no. 9 (2021): 498. http://dx.doi.org/10.3390/md19090498.

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Increasing frequency of native jellyfish proliferations and massive appearance of non-indigenous jellyfish species recently concur to impact Mediterranean coastal ecosystems and human activities at sea. Nonetheless, jellyfish biomass may represent an exploitable novel resource to coastal communities, with reference to its potential use in the pharmaceutical, nutritional, and nutraceutical Blue Growth sectors. The zooxanthellate jellyfish Cassiopea andromeda, Forsskål, 1775 (Cnidaria, Rhizostomeae) entered the Levant Sea through the Suez Canal and spread towards the Western Mediterranean to rea

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Hetti Arachchillage Chathuri Jithmini Hettiarachchi and Darshika Pathiraje. "Harnessing dietary fiber from yellow passion fruit peel in emulsified chicken meat model." World Journal of Biology Pharmacy and Health Sciences 21, no. 1 (2025): 043–55. https://doi.org/10.30574/wjbphs.2025.21.1.1092.

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This study investigated the extraction and application of dietary fiber (DF) from yellow passion fruit peel (YPFP) to enhance the nutritional and functional properties of meat products. The experiments focused on analyzing the physicochemical and functional properties of DF-rich powders extracted using different methods: water bath heating-assisted acidic extraction, ultrasonication-assisted acidic extraction, and enzymatic extraction. The results showed that the total dietary fiber (TDF) content increased by 15.25–22.33% compared to whole peel powder, with the highest TDF content observed

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Cwalina, B., A. Turek, J. Nozynski, M. Jastrzebska, and Z. Nawrat. "Structural Changes in Pericardium Tissue Modified with Tannic Acid." International Journal of Artificial Organs 28, no. 6 (2005): 648–53. http://dx.doi.org/10.1177/039139880502800614.

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Introduction Structural modification of proteins, mainly collagen in connective tissues, is important in the manufacture of tissue-derived biomaterials. Natural compounds like genipin or tannic acid (TA) have been proposed instead of glutaraldehyde which shows cytotoxic effects on the processed tissue. Furthermore, calcification of glutaraldehyde-treated tissue limits the functional lifetime of bioprostheses. TA is known to form numerous hydrogen bonds with proteins. The purpose of our study was to investigate structural changes in porcine pericardium upon chemical modification with tannic aci

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Sato, Kenji, Koji Iwai, and Misako Aito-Inoue. "Identification of Food-Derived Bioactive Peptides in Blood and Other Biological Samples." Journal of AOAC INTERNATIONAL 91, no. 4 (2008): 995–1001. http://dx.doi.org/10.1093/jaoac/91.4.995.

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Abstract Recent studies have demonstrated the presence of food-derived peptides in human blood after ingestion of enzymatic hydrolysates of food proteins, while most peptides in food are degraded into amino acids during digestion and absorption. To capture and clarify the food-derived peptides in blood, solid-phase extraction (SPE) using a mini-spin column packed with a strong cation exchanger was developed. This technique allows the use of a nonvolatile acid such as trichloroacetic acid, a strong protein denaturant, for the deproteinizing procedure. To improve resolution of hydrophilic peptid

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