Academic literature on the topic 'Enzyme-assisted extraction'

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Journal articles on the topic "Enzyme-assisted extraction"

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Liang, Li, Yang Xiaoyu, Pan Lei, Su Yue, and Wang Yan. "Comparing three Methods of Extraction of Auricularia Auricula Polysaccharides." Current Topics in Nutraceutical Research 17, no. 2 (2017): 7–10. http://dx.doi.org/10.37290/ctnr2641-452x.17:7-10.

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Auricularia auricula are rich source of bioactive polysaccharides. Three methods of extraction of polysaccharide from these fungi was studied. These methods included, water-based, ultrasonic-assisted, and enzyme-based extractions. The results showed that the enzyme-based extraction was better than ultrasonic-assisted extraction and water extraction. While abundance of polysaccharide concentration in A. auricula-judae and A. delicata were similar, their yield following enzyme-based extractions were higher than that of A. polytricha. The maximum A. auricula-judae polysaccharide concentration in
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Divković, Laura, Aida Smajlagić, Emina Ahmetović, et al. "Comparative Analysis of Soxhlet and Ultrasound-assisted Extraction of Bioactive Components from Fig Leaves (lat. Ficus carica): Impact of the Method on Extraction Yield and Latex Preservation." International Research Journal of Pure and Applied Chemistry 26, no. 4 (2025): 14–26. https://doi.org/10.9734/irjpac/2025/v26i4924.

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This research investigates the efficiency of two methods for extracting bioactive compounds from fig leaves (Ficus carica) using different solvents. Dried fig leaves were used as extraction material. The focus is on Soxhlet extraction and Ultrasound-assisted extraction (UAE) methods. Also, two different solvents, methanol and ethanol, were used for extractions. Given that the enzyme ficin is one of the significant bioactive compounds in fig leaf latex, the aim was to explore and determine which method and solvent gives a higher extraction yield and better preservation of thermolabile component
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Liu, Longlong, and Hua Zhang. "Milk Thistle Oil Extracted by Enzyme-Mediated Assisted Solvent Extraction Compared with n-Hexane and Cold-Pressed Extraction." Molecules 28, no. 6 (2023): 2591. http://dx.doi.org/10.3390/molecules28062591.

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Silymarin and milk thistle oil have unique biological benefits; however, applying silymarin to milk thistle oil remains a challenge. In this research, the content of silymarin in milk thistle oil conditions using enzyme-mediated solvent extraction was investigated and optimized by response surface methodology. The optimal extraction conditions using enzyme-mediated solvent extraction were as follows: the enzyme-added content was 3.06 mg/mL, the enzymatic hydrolysis temperature was 55.09 °C, and the enzymatic hydrolysis time was 66.28 min. Oil extracted by the enzyme-mediated assisted solvent w
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Muhammad, Dimas Rahadian Aji, Siham Ayouaz, Annisa Noor Rachmawati, et al. "Advanced and Potential Methods for Extraction of Bioactive Compounds from Avocado Peel—A Review." Applied Sciences 14, no. 14 (2024): 6018. http://dx.doi.org/10.3390/app14146018.

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Extraction techniques are continuously developed by the scientific community. Meanwhile, avocado peel is a by-product of avocado processing and a source of bioactive compounds. The purpose of this review was to summarize the use of advanced techniques for extracting bioactive compounds from avocado peel to help understand which techniques have and have not been applied to avocado peel. Three primary databases were used to collect the information, including Google Scholar, Scopus, and Web of Science, by using the keywords “avocado”, “peel”, and “extraction”. Additional keywords related to the e
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Singh, Gurpreet, Mohit Kumar, Ruchika Zalpouri, Pratik Pandit Potdar, Kamalpreet Singh, and Kulwinder Kaur. "Effects of different aqueous extraction techniques on physicochemical quality and oil recovery of sesame oil." Environment Conservation Journal 24, no. 1 (2023): 136–42. http://dx.doi.org/10.36953/ecj.11892309.

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Sesame is the oldest oilseed crop in agriculture, and it produces more oil than any other crop on the planet. This research aimed to investigate the impact of different oil extraction procedures on sesame seed oil physicochemical quality and oil recovery. The oil was extracted from the clean and healthy seeds using four extraction methods: aqueous, enzyme-assisted aqueous, ultrasound-assisted aqueous and solvent extraction using the Soxhlet apparatus. It was observed that ultrasound-assisted aqueous extracted oil had maximum saponification value and minimum acid value, refractive index, and lo
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Qadir, Rahman, Farooq Anwar, Mazhar Amjad Gilani, Sadaf Zahoor, Muhammad Misbah ur Rehman, and Muhammad Mustaqeem. "RSM/ANN based optimized recovery of phenolics from mulberry leaves by enzyme-assisted extraction." Czech Journal of Food Sciences 37, No. 2 (2019): 99–105. http://dx.doi.org/10.17221/147/2018-cjfs.

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Recovery of phenolics from Morus alba leaves (MAL) and extraction into the solvent was optimized using enzyme-assisted extraction. The influence of four parameters, including enzyme concentration (EC), temperature (T), incubation time (t) and pH were investigated using rotatable central composite design (RCCD). Two factors, namely enzyme concentration and pH, exhibited significant effect on extraction efficacy yield of extractable phenolics from MAL. Furthermore, artificial neural network (ANN) model was executed to predict the relationship between dependent and independent variables. Among en
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Wirajana, I. N., N. M. T. Juliasari, A. A. I. A. M. Laksmiwati, and N. W. Bogoriani. "SUHU DAN WAKTU OPTIMUM PROSES EKSTRAKSI ANTOSIANIN DALAM UBI JALAR UNGU (Ipomoea batatas L.) DENGAN ?-L-ARABINOFURANOSIDASE." Jurnal Kimia 13, no. 1 (2019): 88. http://dx.doi.org/10.24843/jchem.2019.v13.i01.p14.

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Enzyme-assisted extraction (EAE) method is one of the most environmentally friendly methods of enzyme application in the extraction of bioactive compounds. The purpose of this study was to determine the optimum temperature and time required in the extraction of anthocyanin compounds from purple sweet potato (Ipomoea batatas L.) with and without ?-L-arabinofuranosidase (AbfA) - assisted. The AbfA enzyme was obtained from Saccharomyces cerevisiae recombinant strain BJ1824 contain pYHMI-Af plasmid. The optimum temperature and time in the extraction of anthocyanin compound with and without ?-L-ara
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Nguyen, Hoang Chinh, Kim Ngan Ngo, Hoai Khang Tran, and Colin J. Barrow. "Enzyme-Assisted Coextraction of Phenolics and Polysaccharides from Padina gymnospora." Marine Drugs 22, no. 1 (2024): 42. http://dx.doi.org/10.3390/md22010042.

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Brown seaweed is a promising source of polysaccharides and phenolics with industrial utility. This work reports the development of a green enzyme-assisted extraction method for simultaneously extracting polysaccharides and phenolics from the brown seaweed Padina gymnospora. Different enzymes (Cellulast, Pectinex, and Alcalase), individually and in combination, were investigated, with Alcalase alone showing the highest efficiency for the simultaneous extraction of polysaccharides and phenolics. Yields from Alcalase-assisted aqueous extraction were higher than those obtained using either water a
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Phan Van, Man, Duy Tran Duc, Hai Dam Thi Thanh, and Hai Tran Chi. "Comparison of ultrasound assisted extraction and enzyme assisted extraction of betacyanin from red dragon fruit peel." E3S Web of Conferences 187 (2020): 04004. http://dx.doi.org/10.1051/e3sconf/202018704004.

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This article was intended to extract betacyanin from the peel of red dragon fruit (Hylocereus polyrhizus) and used it as a natural colorant. In this study, enzyme and ultrasound techniques for the extraction of betacyanin from dried dragon fruit peel were compared. The ultrasonic power and sonication time levels were varied between 0.5, 1.5, 2.5, 3.5, and W/g; and 2.5, 5.0, 7.5, 10.0, and 12.5 min. The enzyme concentrations were 0.25, 0.75, 1.25, 1.75, 2.25, and 2.75 %v/w. The results revealed that the maximum betacyanin content obtained by the optimal UAE condition (3.5 W/g and 7.5 min) was 0
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Damar, İrem. "COMPARATIVE EVALUATION OF DIFFERENT EXTRACTION METHODS FOR ANTHOCYANIN RECOVERY FROM RED ONION PEEL: ENZYME-ASSISTED, ULTRASOUND-ASSISTED, AND ULTRASOUND-ASSISTED ENZYMATIC METHODS." Gıda 50, no. 3 (2025): 389–405. https://doi.org/10.15237/gida.gd25040.

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In this study, enzyme-assisted extraction (EAE), bath and probe type ultrasound-assisted extraction (UAE-B, UAE-P), and ultrasound-assisted enzymatic extraction using bath and probe-type ultrasound (UAEE-B, UAEE-P) were compared to conventional extraction (CE) for recovering anthocyanins from red onion peel. Extracts were analyzed for total phenolic (TPC), total flavonoid (TFC), total monomeric anthocyanin (TMA), antioxidant activities (AA-ABTS, AA-DPPH), individual anthocyanins, and colour parameters. UAEE-B achieved 33.12% higher extraction yield than CE. UAEE-B showed more TPC compared to o
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Dissertations / Theses on the topic "Enzyme-assisted extraction"

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Campbell, Kerry Alan. "Protein and oil recoveries from enzyme-assisted aqueous extraction of soybeans and sunflower seed." [Ames, Iowa : Iowa State University], 2010. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3403074.

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Kapasakalidis, Petros G. "Enzyme assisted extraction of antioxidant phenols and anthocyanins from blackcurrant (Ribes nigrum L.) press residues." Thesis, University of Reading, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433443.

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Li, Yuxiu. "Technology of enzyme extraction of Gynostemma pentaphyllum saponins and studies on its hypoglycemic activity." Магістерська робота, Kyiv National University of Technology and Design, 2021. https://er.knutd.edu.ua/handle/123456789/19271.

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In this master's thesis, the composition of saponins in extracts of Gynostemma pentaphyllum obtained by enzymatic extraction has been determined. The HPLC method was used for the simultaneous analysis of hypenosides in medicinal plant materials of different periods and places of growth. The results showed that the highest content of hypenosides was noted in August, the lowest in May; the composition and content of hypenosides from different production zones in the same growing season were different. The optimal parameters of extraction using hypenosidase are as follows: the time of enzymatic h
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Fournière, Mathilde. "Extraction et évaluation des propriétés biologiques des ulvanes et oligo-ulvanes de l'algue verte Ulva sp. : actions sur le métabolisme de cellules cutanées et sur le microbiote cutané." Electronic Thesis or Diss., Lorient, 2021. http://www.theses.fr/2021LORIS593.

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Les ulvanes sont des polysaccharides matriciels sulfatés constitutifs de la paroi de l’algue verte Ulva sp.. Bien que proliférative et quantitativement disponible, Ulva sp. est peu valorisée avec des applications dans des domaines à faible valeur ajoutée. Ce projet de thèse a pour objectif la production de fractions enrichies en ulvanes et en oligo-ulvanes dans le but d’évaluer leurs propriétés biologiques sur le système cutané. Les fractions d’ulvanes ont été produites par macération et extraction assistée par enzyme suivie de différents procédés de purification : précipitation éthanolique et
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Hardouin, Kévin. "Production d'extraits aqueux à partir d'Ulva sp, au moyen de procédés d'hydrolyse enzymatique : caractérisatin, valorisation et perspectives de développement." Thesis, Lorient, 2015. http://www.theses.fr/2015LORIS373.

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Ces travaux de thèse CIFRE, réalisés au sein du Laboratoire de Biotechnologie et Chimie Marines de l’Université de Bretagne-Sud et du Groupe Olmix, s’inscrivent dans le cadre d’un projet national de valorisation de biomasses d’algues vertes, le projet ULVANS. Ce projet est le fruit d’une collaboration entre cinq industriels bretons et deux laboratoires universitaires. Les objectifs de ces travaux de thèse concernent i) la caractérisation approfondie de la matière première, en particulier des polysaccharides matriciels solubles (ulvanes) et des protéines, ii) la mise au point d’un procédé d’ext
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Davidson, Morag. "Éco-extraction de composés bioactifs à partir de marcs de fruits rouges & étude de leur impact sur l'homéostasie intestinale." Electronic Thesis or Diss., Limoges, 2024. http://www.theses.fr/2024LIMO0020.

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Depuis 2010, 100 millions de tonnes de fruits rouges sont produits chaque année dans le monde. 20% de déchets résultent de leur 1ère transformation industrielle, comprenant les graines et les peaux, appelés marcs. L’épandage et l’alimentation animale constituent leurs principales voies de valorisation, alors qu’ils peuvent être une source potentielle de composés bioactifs hydrophiles (fibres, protéines, polyphénols, oligo-éléments) et lipophiles (acides gras polyinsaturés, phytostérols, tocols). Par conséquent, il devient intéressant de concevoir des procédés d’extraction simultanée de l’ensem
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Yu, Dajun. "Ultrasound-assisted enzymatic extraction of protein hydrolysates from brewer's spent grain." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/97875.

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Brewer's spent grain (BSG) is the most abundant by-product of the brewing industry and its main application is limited to low-value cattle feed. Since BSG contains 20 to 25% of proteins, it has the potential to provide a new protein source to the food industry. In this research, an ultrasound-assisted enzymatic extraction was designed to extract protein hydrolysates from BSG. Original BSG and ultrasound pretreated BSG were hydrolyzed under different enzyme (Alcalase) loadings and incubation times. Centrifugation was applied to separate solubilized proteins from insoluble BSG residue. When the
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ZENG, JING-FEN, and 曾靜芬. "Enzyme-assisted Extraction of Phenolic Compounds From Kumquat and Its Biological Activities." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/z7f89b.

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碩士<br>國立宜蘭大學<br>食品科學系碩士班<br>106<br>Kumquat is one of the important agricultural crops in Yilan. It is well known that kumquat have anti-microbial, antioxidant and tyrosinase inhibitory activities, which might because of the presence of phenolic compounds. Therefore, effective extraction of phenolic compounds from kumquat could promote the application and its additional commercial value. Enzymes-assisted extraction method can increase the extraction yield of phenolic compounds, and also reduce the solvent usage and lowering environment risk. Thus, the aim of this study was to develop extraction
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NAIR, ADITHYA. "ENZYME ASSISTED AQUEOUS EXTRACTION OF VEGETABLE OIL AND ENZYMATIC TREATMENT OF VEGETABLE OIL MILL EFFLUENT." Thesis, 2018. http://dspace.dtu.ac.in:8080/jspui/handle/repository/16422.

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Vegetable oil represents a major share in the global consumable fats and oil produced. The major types of vegetable oil produced are Palm oil, Soybean oil, Canola oil etc. with palm accounting for more than 30% of the entire production. Conventional oil extraction steps include mechanical and solvent extraction. An alternative for these conventional steps of oil extraction is the aqueous enzymatic extraction, here the solvent used for extracting the oil is water and thus it minimises the many environmental hazards posed by solvent extraction. The enzymatic extraction involves use of a combinat
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Yeh, Yu-Cheng, and 葉祐成. "Studies on the physicochemical properties of mucilage from the seed of Citrus grandis Osbeck with enzyme or enzyme combined with ultrasonic assisted extraction and its application in edible film." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5253035%22.&searchmode=basic.

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碩士<br>國立中興大學<br>食品暨應用生物科技學系所<br>107<br>Mucilage is a hydrocolloid, mainly composed of polysaccharide. It is applied to the modification of food properties. In previous studies, there is an abundant pectin-like mucilage in seed coat of Citrus grandis Osbeck. Therefore, the purpose of this study is to characterize the physicochemical properties of mucilage of Citrus grandis Osbeck seed through enzymatic and ultrasonic extraction methods. The results showed that the yield increased after enzymatic or ultrasonic treatment. Especially celluclast combined with ultrasound, it could significantly incr
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Book chapters on the topic "Enzyme-assisted extraction"

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Maled, Sadhana B., Ajay R. Bhat, Subrahmanya Hegde, Yuvaraj Sivamani, Arunachalam Muthuraman, and Sumitha Elayaperumal. "Enzyme-Assisted Extraction." In Bioactive Extraction and Application in Food and Nutraceutical Industries. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3601-5_8.

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Marathe, Sandesh J., Swati B. Jadhav, Sandip B. Bankar, and Rekha S. Singhal. "Enzyme-Assisted Extraction of Bioactives." In Food Bioactives. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-51639-4_8.

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Kleekayai, Thanyaporn, Mohammadreza Khalesi, Miryam Amigo-Benavent, Maria Cermeño, Pádraigín Harnedy-Rothwell, and Richard J. FitzGerald. "Enzyme-Assisted Extraction of Plant Proteins." In Green Protein Processing Technologies from Plants. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-16968-7_6.

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Das, Srija, Shamraja S. Nadar, and Virendra K. Rathod. "Chapter 7. Enzyme-assisted Extraction of Bioactive Ingredients." In Green Chemistry Series, 2nd ed. Royal Society of Chemistry, 2022. http://dx.doi.org/10.1039/9781839165894-00269.

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Chaminda Lakmal, H. H., Kalpa W. Samarakoon, and You-Jin Jeon. "Enzyme-Assisted Extraction to Prepare Bioactive Peptides from Microalgae." In Marine Algae Extracts. Wiley-VCH Verlag GmbH & Co. KGaA, 2015. http://dx.doi.org/10.1002/9783527679577.ch18.

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Jeon, You-Jin, W. A. J. P. Wijesinghe, and Se-Kwon Kim. "Enzyme-assisted Extraction and Recovery of Bioactive Components from Seaweeds." In Handbook of Marine Macroalgae. John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9781119977087.ch10.

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Lakmal, H. H. Chaminda, Ji-Hyeok Lee, and You-Jin Jeon. "Enzyme-Assisted Extraction of a Marine Algal Polysaccharide, Fucoidan and Bioactivities." In Polysaccharides. Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-03751-6_46-1.

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Mohamed, N. A., W. Y. Wan Ahmad, K. Ngalib, M. R. Ahmad, M. I. Ab Kadir, and A. Ismail. "Microwave-Enzyme-Assisted Extraction and Dyeing of Lichen Species: Parmotrema praesorediosum." In Proceedings of the International Colloquium in Textile Engineering, Fashion, Apparel and Design 2014 (ICTEFAD 2014). Springer Singapore, 2014. http://dx.doi.org/10.1007/978-981-287-011-7_16.

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Lakmal, H. H. Chaminda, Ji-Hyeok Lee, and You-Jin Jeon. "Enzyme-Assisted Extraction of a Marine Algal Polysaccharide, Fucoidan and Bioactivities." In Polysaccharides. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16298-0_46.

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Casas, María Parada, and Herminia Domínguez González. "Enzyme-Assisted Aqueous Extraction Processes." In Water Extraction of Bioactive Compounds. Elsevier, 2017. http://dx.doi.org/10.1016/b978-0-12-809380-1.00013-9.

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Conference papers on the topic "Enzyme-assisted extraction"

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Kim, Youngmi, and Virginia Lee. "Enzyme-assisted Protein Extraction from Alfalfa Leaves." In 2021 ASABE Annual International Virtual Meeting, July 12-16, 2021. American Society of Agricultural and Biological Engineers, 2021. http://dx.doi.org/10.13031/aim.202100024.

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Cheng, Xian, Liangwu Bi, Zhendong Zhao, and Yuxiang Chen. "Advances in Enzyme Assisted Extraction of Natural Products." In 3rd International Conference on Material, Mechanical and Manufacturing Engineering (IC3ME 2015). Atlantis Press, 2015. http://dx.doi.org/10.2991/ic3me-15.2015.72.

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"Techno-Economic Analysis of Integrated Enzyme Assisted Aqueous Extraction of Soybean Oil." In 2016 ASABE International Meeting. American Society of Agricultural and Biological Engineers, 2016. http://dx.doi.org/10.13031/aim.20162459761.

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"Environmental Impact Analysis of Soybean Oil Production from Expelling, Hexane Extraction and Enzyme Assisted Aqueous Extraction." In 2016 ASABE International Meeting. American Society of Agricultural and Biological Engineers, 2016. http://dx.doi.org/10.13031/aim.20162459781.

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Sarasini, F., J. Tirillò, G. Maffei, A. Zuorro, R. Lavecchia, and M. Abdelhak. "Enzyme-assisted extraction of fibres from Ampelodesmos mauritanicus and mechanical characterization of their composites." In 9TH INTERNATIONAL CONFERENCE ON “TIMES OF POLYMERS AND COMPOSITES”: From Aerospace to Nanotechnology. Author(s), 2018. http://dx.doi.org/10.1063/1.5045882.

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Yang, Jasmin, Fernanda Furlan Goncalves Dias, and Juliana M. Leite Nobrega De Moura Bell. "Sequential Fractionation as a Tool for Understanding the Physicochemical and Thermal Properties of Aqueous and Enzyme-assisted Aqueous Extracted Black Bean Proteins." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/qivq4253.

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Black bean proteins were sequentially extracted and characterized to understand the contribution of different protein classes to the physicochemical and thermal properties of black bean protein extracts produced with commercially-feasible extraction processes (i.e., aqueous extraction process, AEP, and enzyme-assisted aqueous extraction process, EAEP). The Osborne fractionation method was used to produce protein fractions rich in albumins (56%), globulins (22%), prolamins (0.65%), and glutelins (16%). AEP (pH 9.0, 50 °C, 1:10 solids-to-liquid ratio, 60 min) and EAEP (same conditions as AEP, ex
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Chang, Shu-Wei, and Tsai-Wei Huang. "Study on the Effect of Enzyme Assisted Method on Extraction Efficiency of Polysaccharides from Seaweed." In Virtual 2021 AOCS Annual Meeting & Expo. American Oil Chemists’ Society (AOCS), 2021. http://dx.doi.org/10.21748/am21.512.

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Alpiger, Simone Bleibach, and Milena Corredig. "Influence of Process Conditions on Enzyme Assisted Aqueous Extraction of Protein from Whole Rapeseed Using Pectinase." In Virtual 2021 AOCS Annual Meeting & Expo. American Oil Chemists’ Society (AOCS), 2021. http://dx.doi.org/10.21748/am21.51.

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Lamsal, Buddhi, and Md Mahfuzur Rahman. "Conventional and novel technologies for extraction of protein and their impact on structure and functionality as ingredient." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/dhxf1174.

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Proteins possess their techno-functionalities by virtues of their state of being, i.e., their molecular makeup and structure, which in turn, is affected by the technologies employed to extract them from the matrices they belong to. This is true for both plant proteins and cell-based proteins. While pH-modulated solubility based aqueous extraction, followed by isolation, is the overwhelming method for plant protein preparations, other technologies, for example dry fractionation (separation based on density, air drag or electrostatic charges), enzyme-, microwave-, ultrasound-, pulsed electric en
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Hallstrom, Jessica, Fernanda Furlan Goncalves Dias, and Juliana Leite Nobrega De Moura Bell. "Impact of Almond Roasting and Particle Size on the Simultaneous Extraction of Lipids and Proteins for Almond Milk Production." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/jcmm2165.

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Almond milk consumption has increased as an alternative to dairy milk. However, its low nutrient density and presence of emulsifiers have negatively impacted its consumer perception. We evaluated two sustainable approaches: aqueous (AEP) and enzyme-assisted extraction processes (EAEP), to produce a more nutrient-dense and functional milk. The effects of almond roasting, particle size, and extraction methods (AEP vs. EAEP) were evaluated on protein and oil extractability and in vitro protein digestibility. Unroasted and “light” and “dark” roasted almonds were ground to different particle sizes
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