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Journal articles on the topic 'Enzyme-assisted extraction'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Liang, Li, Yang Xiaoyu, Pan Lei, Su Yue, and Wang Yan. "Comparing three Methods of Extraction of Auricularia Auricula Polysaccharides." Current Topics in Nutraceutical Research 17, no. 2 (2017): 7–10. http://dx.doi.org/10.37290/ctnr2641-452x.17:7-10.

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Auricularia auricula are rich source of bioactive polysaccharides. Three methods of extraction of polysaccharide from these fungi was studied. These methods included, water-based, ultrasonic-assisted, and enzyme-based extractions. The results showed that the enzyme-based extraction was better than ultrasonic-assisted extraction and water extraction. While abundance of polysaccharide concentration in A. auricula-judae and A. delicata were similar, their yield following enzyme-based extractions were higher than that of A. polytricha. The maximum A. auricula-judae polysaccharide concentration in

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2

Divković, Laura, Aida Smajlagić, Emina Ahmetović, et al. "Comparative Analysis of Soxhlet and Ultrasound-assisted Extraction of Bioactive Components from Fig Leaves (lat. Ficus carica): Impact of the Method on Extraction Yield and Latex Preservation." International Research Journal of Pure and Applied Chemistry 26, no. 4 (2025): 14–26. https://doi.org/10.9734/irjpac/2025/v26i4924.

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This research investigates the efficiency of two methods for extracting bioactive compounds from fig leaves (Ficus carica) using different solvents. Dried fig leaves were used as extraction material. The focus is on Soxhlet extraction and Ultrasound-assisted extraction (UAE) methods. Also, two different solvents, methanol and ethanol, were used for extractions. Given that the enzyme ficin is one of the significant bioactive compounds in fig leaf latex, the aim was to explore and determine which method and solvent gives a higher extraction yield and better preservation of thermolabile component

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3

Liu, Longlong, and Hua Zhang. "Milk Thistle Oil Extracted by Enzyme-Mediated Assisted Solvent Extraction Compared with n-Hexane and Cold-Pressed Extraction." Molecules 28, no. 6 (2023): 2591. http://dx.doi.org/10.3390/molecules28062591.

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Silymarin and milk thistle oil have unique biological benefits; however, applying silymarin to milk thistle oil remains a challenge. In this research, the content of silymarin in milk thistle oil conditions using enzyme-mediated solvent extraction was investigated and optimized by response surface methodology. The optimal extraction conditions using enzyme-mediated solvent extraction were as follows: the enzyme-added content was 3.06 mg/mL, the enzymatic hydrolysis temperature was 55.09 °C, and the enzymatic hydrolysis time was 66.28 min. Oil extracted by the enzyme-mediated assisted solvent w

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4

Muhammad, Dimas Rahadian Aji, Siham Ayouaz, Annisa Noor Rachmawati, et al. "Advanced and Potential Methods for Extraction of Bioactive Compounds from Avocado Peel—A Review." Applied Sciences 14, no. 14 (2024): 6018. http://dx.doi.org/10.3390/app14146018.

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Extraction techniques are continuously developed by the scientific community. Meanwhile, avocado peel is a by-product of avocado processing and a source of bioactive compounds. The purpose of this review was to summarize the use of advanced techniques for extracting bioactive compounds from avocado peel to help understand which techniques have and have not been applied to avocado peel. Three primary databases were used to collect the information, including Google Scholar, Scopus, and Web of Science, by using the keywords “avocado”, “peel”, and “extraction”. Additional keywords related to the e

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Singh, Gurpreet, Mohit Kumar, Ruchika Zalpouri, Pratik Pandit Potdar, Kamalpreet Singh, and Kulwinder Kaur. "Effects of different aqueous extraction techniques on physicochemical quality and oil recovery of sesame oil." Environment Conservation Journal 24, no. 1 (2023): 136–42. http://dx.doi.org/10.36953/ecj.11892309.

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Sesame is the oldest oilseed crop in agriculture, and it produces more oil than any other crop on the planet. This research aimed to investigate the impact of different oil extraction procedures on sesame seed oil physicochemical quality and oil recovery. The oil was extracted from the clean and healthy seeds using four extraction methods: aqueous, enzyme-assisted aqueous, ultrasound-assisted aqueous and solvent extraction using the Soxhlet apparatus. It was observed that ultrasound-assisted aqueous extracted oil had maximum saponification value and minimum acid value, refractive index, and lo

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Qadir, Rahman, Farooq Anwar, Mazhar Amjad Gilani, Sadaf Zahoor, Muhammad Misbah ur Rehman, and Muhammad Mustaqeem. "RSM/ANN based optimized recovery of phenolics from mulberry leaves by enzyme-assisted extraction." Czech Journal of Food Sciences 37, No. 2 (2019): 99–105. http://dx.doi.org/10.17221/147/2018-cjfs.

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Recovery of phenolics from Morus alba leaves (MAL) and extraction into the solvent was optimized using enzyme-assisted extraction. The influence of four parameters, including enzyme concentration (EC), temperature (T), incubation time (t) and pH were investigated using rotatable central composite design (RCCD). Two factors, namely enzyme concentration and pH, exhibited significant effect on extraction efficacy yield of extractable phenolics from MAL. Furthermore, artificial neural network (ANN) model was executed to predict the relationship between dependent and independent variables. Among en

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Wirajana, I. N., N. M. T. Juliasari, A. A. I. A. M. Laksmiwati, and N. W. Bogoriani. "SUHU DAN WAKTU OPTIMUM PROSES EKSTRAKSI ANTOSIANIN DALAM UBI JALAR UNGU (Ipomoea batatas L.) DENGAN ?-L-ARABINOFURANOSIDASE." Jurnal Kimia 13, no. 1 (2019): 88. http://dx.doi.org/10.24843/jchem.2019.v13.i01.p14.

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Enzyme-assisted extraction (EAE) method is one of the most environmentally friendly methods of enzyme application in the extraction of bioactive compounds. The purpose of this study was to determine the optimum temperature and time required in the extraction of anthocyanin compounds from purple sweet potato (Ipomoea batatas L.) with and without ?-L-arabinofuranosidase (AbfA) - assisted. The AbfA enzyme was obtained from Saccharomyces cerevisiae recombinant strain BJ1824 contain pYHMI-Af plasmid. The optimum temperature and time in the extraction of anthocyanin compound with and without ?-L-ara

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8

Nguyen, Hoang Chinh, Kim Ngan Ngo, Hoai Khang Tran, and Colin J. Barrow. "Enzyme-Assisted Coextraction of Phenolics and Polysaccharides from Padina gymnospora." Marine Drugs 22, no. 1 (2024): 42. http://dx.doi.org/10.3390/md22010042.

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Brown seaweed is a promising source of polysaccharides and phenolics with industrial utility. This work reports the development of a green enzyme-assisted extraction method for simultaneously extracting polysaccharides and phenolics from the brown seaweed Padina gymnospora. Different enzymes (Cellulast, Pectinex, and Alcalase), individually and in combination, were investigated, with Alcalase alone showing the highest efficiency for the simultaneous extraction of polysaccharides and phenolics. Yields from Alcalase-assisted aqueous extraction were higher than those obtained using either water a

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9

Phan Van, Man, Duy Tran Duc, Hai Dam Thi Thanh, and Hai Tran Chi. "Comparison of ultrasound assisted extraction and enzyme assisted extraction of betacyanin from red dragon fruit peel." E3S Web of Conferences 187 (2020): 04004. http://dx.doi.org/10.1051/e3sconf/202018704004.

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This article was intended to extract betacyanin from the peel of red dragon fruit (Hylocereus polyrhizus) and used it as a natural colorant. In this study, enzyme and ultrasound techniques for the extraction of betacyanin from dried dragon fruit peel were compared. The ultrasonic power and sonication time levels were varied between 0.5, 1.5, 2.5, 3.5, and W/g; and 2.5, 5.0, 7.5, 10.0, and 12.5 min. The enzyme concentrations were 0.25, 0.75, 1.25, 1.75, 2.25, and 2.75 %v/w. The results revealed that the maximum betacyanin content obtained by the optimal UAE condition (3.5 W/g and 7.5 min) was 0

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10

Damar, İrem. "COMPARATIVE EVALUATION OF DIFFERENT EXTRACTION METHODS FOR ANTHOCYANIN RECOVERY FROM RED ONION PEEL: ENZYME-ASSISTED, ULTRASOUND-ASSISTED, AND ULTRASOUND-ASSISTED ENZYMATIC METHODS." Gıda 50, no. 3 (2025): 389–405. https://doi.org/10.15237/gida.gd25040.

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In this study, enzyme-assisted extraction (EAE), bath and probe type ultrasound-assisted extraction (UAE-B, UAE-P), and ultrasound-assisted enzymatic extraction using bath and probe-type ultrasound (UAEE-B, UAEE-P) were compared to conventional extraction (CE) for recovering anthocyanins from red onion peel. Extracts were analyzed for total phenolic (TPC), total flavonoid (TFC), total monomeric anthocyanin (TMA), antioxidant activities (AA-ABTS, AA-DPPH), individual anthocyanins, and colour parameters. UAEE-B achieved 33.12% higher extraction yield than CE. UAEE-B showed more TPC compared to o

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Tamang, Bunu, Qi Liang, Biju Balakrishnan, Su Peng, and Wei Zhang. "Astragalus Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line." Nutrients 14, no. 11 (2022): 2333. http://dx.doi.org/10.3390/nu14112333.

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The chemical and nutritional constituents of mushrooms can alter significantly when grown on different substrates. Based on this fact, an approach was made to cultivate a new type of mushroom, Hengshan Astragalus Shiitake, by growing Shiitake mushrooms on beds supplemented with the roots of an edible herbal plant, Astragalus membranaceus. In this study, three green extraction techniques, including microwave-enzyme assisted (MEA), ultrasound-enzyme assisted (UEA) and microwave-ultrasound-enzyme assisted (MUEA) extractions, were used to compare both the yield and antiproliferative activity of th

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Výbohová, Veronika, and Katarína Hroboňová. "Isolation of Natural Compounds with Organoleptic Properties Using Selected Advanced Extraction Techniques." Chemické listy 117, no. 6 (2023): 365–72. http://dx.doi.org/10.54779/chl20230365.

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This review presents an overview of various advanced solid–liquid extraction techniques used for extraction of compounds with organoleptic properties from natural sources. These compounds are important additives to food, cosmetic, and pharmaceutical products. The most frequently used techniques are ultrasound assisted extraction and microwave assisted one, but there are other efficient techniques, such as pressurized liquid extraction, supercritical fluid extraction, pulsed electric field and enzyme assisted extraction. These techniques reduce or eliminate the volume of toxic solvents required

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13

Tran Chi Hai, Mai Tien Hung, Chu Thi Nga, et al. "ENZYME-ASSISTED EXTRACTION OF TRITERPENOID AND PHENOLIC COMPOUNDS FROM Rubus alceaefolius Poir LEAVES." Tạp chí Khoa học Đại học Công Thương 24, no. 3 (2024): 3–13. http://dx.doi.org/10.62985/j.huit_ojs.vol24.no3.81.

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The leaves of Rubus alceaefolius Poir primarily consist of phenolic and triterpenoid compounds, which possess a variety of biological activities beneficial to human health. This research aimed to explore the impact of enzyme-assisted extraction conditions on the recovery of triterpenoid and phenolic compounds from Rubus alceaefolius Poir leaves. Factors considered in the enzymatic treatment process with Viscozyme L cellulase included the solid-to-water ratio (1:15-1:45 w/v), enzyme concentration (0.5-3.5%), temperature (40-60 °C), and extraction duration (30-180 min). The results suggested tha

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Stanek-Wandzel, Natalia, Alicja Krzyszowska, Magdalena Zarębska, et al. "Evaluation of Cellulase, Pectinase, and Hemicellulase Effectiveness in Extraction of Phenolic Compounds from Grape Pomace." International Journal of Molecular Sciences 25, no. 24 (2024): 13538. https://doi.org/10.3390/ijms252413538.

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Grape pomace, the solid residue from winemaking, is a rich source of polyphenolic compounds with significant antioxidant properties. However, the efficient extraction of these valuable compounds remains a challenge. This study focuses on optimizing the conditions for the enzyme-assisted extraction of polyphenolic compounds from red grape pomace using cellulase, hemicellulase, and pectinase. The key variables investigated in this study were enzyme concentration, extraction time, and solid/liquid ratio. The results highlight the importance of selecting enzymes based on target compounds, as diffe

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15

Li, Yang, Lian Zhou Jiang, Chen Wang, and Xiao Nan Sui. "The Comparison of Oil Quality from Different Processes." Applied Mechanics and Materials 66-68 (July 2011): 598–607. http://dx.doi.org/10.4028/www.scientific.net/amm.66-68.598.

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In this study, different oil extraction processes, such as extrusion pretreatment, ultrasound-ethanol assisted demulsification and traditional hexane extraction of oil, were employed to extract oil from soybean in order to compare their different effects on oil quality, fatty acid distribution and VE content of oil. The result shows that the quality of oil from extrusion pretreatment aqueous enzyme extraction (EAEP) of oil and ultrasound-ethanol assisted demulsification aqueous enzyme extraction of oil were same. The raw oil quality from the above two processes was better than the hexane extra

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16

Chen, Yang, Gonglin Cai, Hufu Chen, Huabin Zhou, Hang Qu, and Hailong Yang. "Physicochemical Properties and Biological Activities of Polysaccharides from Dendrobium officinale Leaves in Response to Different Extraction Methods." Foods 14, no. 12 (2025): 2029. https://doi.org/10.3390/foods14122029.

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Extraction techniques play a crucial role in determining the structural attributes and biological functions of polysaccharides. This study aimed to evaluate the physicochemical and biological properties of Dendrobium officinale leaf polysaccharides (DLPs) extracted using various methods, including hot water, acidic, alkali, ultrasound-assisted, and enzyme-assisted extraction. The results indicated that the extraction methods significantly influenced the yield, content, molar ratios of monosaccharides, molecular weight, and structural features of the polysaccharides, as well as their in vitro a

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17

TAKAHASHI, Minako, Naoki AZUMA, and Yoshitsugu KOSUGI. "Enzyme-Assisted Extraction of Rice Bran Oil." Journal of Japan Oil Chemists' Society 48, no. 2 (1999): 143–46. http://dx.doi.org/10.5650/jos1996.48.143.

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18

Puri, Munish, Deepika Sharma, and Colin J. Barrow. "Enzyme-assisted extraction of bioactives from plants." Trends in Biotechnology 30, no. 1 (2012): 37–44. http://dx.doi.org/10.1016/j.tibtech.2011.06.014.

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Sharma, Aparna, S. K. Khare, and M. N. Gupta. "Enzyme-assisted aqueous extraction of peanut oil." Journal of the American Oil Chemists' Society 79, no. 3 (2002): 215–18. http://dx.doi.org/10.1007/s11746-002-0463-0.

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20

Xiong, Jun Juan, and Li Jun Ding. "Optimised Aspergillus Niger Enzyme-Assisted Extraction of Flavonoids from Dicranopteris and Evaluation of Antioxidant Activity In Vitro." Advanced Materials Research 396-398 (November 2011): 1436–39. http://dx.doi.org/10.4028/www.scientific.net/amr.396-398.1436.

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The orthogonal experimental design was used to optimise Aspergillus niger enzyme-assisted extraction for flavonoids-enriched extract from Dicranopteris. The results indicate that the highest extraction ratio of flavonoids by Aspergillus niger enzyme-assisted extraction could arrive 13.67% using 30ml Aspergillus niger enzyme solution at 70°C,pH5.0 for 2h extraction, which was more efficient than that by without enzymes heating extraction.The flavonoids extract exhibited quite excellent inhibitions of hydroxyl radical. Meanwhile, it was found that the bioactivities appeared to be dose-dependent

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21

Nam, Dong-Geon, Mina Kim, Jeong-Sook Choe, and Ae-jin Choi. "Effects of High-Pressure, Hydrothermal, and Enzyme-Assisted Treatment on the Taste and Flavor Profile of Water-Soluble Ginger (Zingiber officinale) Extract." Foods 11, no. 4 (2022): 508. http://dx.doi.org/10.3390/foods11040508.

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Ginger, a plant widely consumed worldwide, is used as a spice or to enhance the flavor of foods. In this study, the taste characteristics (gingerol, shogaol, and amino acid) of extracts treated with various solubilizing methods were objectively compared. In addition, an E-nose confirmed the flavor pattern combined with principal component analysis (PCA) between each extract gas chromatogram-tandem mass spectrometry was performed to compare and analyze volatile compounds between extraction methods. As a result, high-pressure enzyme-assisted extraction (HPE) and hydrothermal enzyme-assisted extr

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22

Oanh, Doan Thi Yen, Ly Thi Minh Hien, Nguyen Kha Duyen, Nguyen Huu Hieu, and Dong Thi Anh Dao. "Effect of enzyme – assisted extraction on total polyphenol content and antioxidant capacity from fresh tea leaves (Camellia sinensis)." Vietnam Journal of Chemistry 61, no. 5 (2023): 551–62. http://dx.doi.org/10.1002/vjch.202300109.

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AbstractTea leaves contain high concentration of polyphenols, known as bioactive compounds. In order to increase the extraction yield of polyphenol and the antioxidant capacity of the extract from tea leaves, the combination of cellulase, pectinase and protease in enzyme‐assisted extraction was investigated. By using fractional factorial experimental design to study the two‐enzyme assisted extraction of cellulase and pectinase, three factors including solvent/material ratio, pH and hydrolysis time showed significant effects on the extract properties. The highest total polyphenol content obtain

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Thirugnanasambandham, K. "Mathematical Modeling and Simulation of Enzyme Assisted Bioactive Compound Extraction from Allium Cepa Using Response Surface Methodology." Asian Journal of Engineering and Applied Technology 7, no. 1 (2018): 43–48. http://dx.doi.org/10.51983/ajeat-2018.7.1.979.

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In this present study, an attempt was made to investigate the enzyme assisted extraction process to extract the phenolic compounds from onion (Allium cepa) under various operating conditions such as temperature, extraction time and enzyme concentration. Three factors three level Box-Behnken response surface design (BBD) coupled with desired function methodlogy was used to optimize and model the extraction process. Optimum extracting conditions for the maximum phenloic compounds were determined and it was found to be: temperature of 40 0C, extraction time of 16 hour and enzyme concentration of

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Usman, Muhammad, Mayuko Nakagawa, and Shuo Cheng. "Emerging Trends in Green Extraction Techniques for Bioactive Natural Products." Processes 11, no. 12 (2023): 3444. http://dx.doi.org/10.3390/pr11123444.

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This review explores eco-friendly methods for extracting bioactive natural products from diverse sources. The introductory exploration emphasizes the increasing demand for sustainable extraction methods, with a focus on the environmental impact of conventional approaches. Addressing existing knowledge gaps, this review outlines the key objectives of evaluating various green extraction technologies, including supercritical fluid extraction, pressurized liquid extraction, ultrasound-assisted extraction, enzyme-assisted extraction, and others. The primary findings underscore the remarkable potent

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Nguyen, Thuan Thi, Maria Dalgaard Mikkelsen, Vy Ha Nguyen Tran, et al. "Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima." Marine Drugs 18, no. 6 (2020): 296. http://dx.doi.org/10.3390/md18060296.

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Fucoidans from brown macroalgae (brown seaweeds) have different structures and many interesting bioactivities. Fucoidans are classically extracted from brown seaweeds by hot acidic extraction. Here, we report a new targeted enzyme-assisted methodology for fucoidan extraction from brown seaweeds. This enzyme-assisted extraction protocol involves a one-step combined use of a commercial cellulase preparation (Cellic®CTec2) and an alginate lyase from Sphingomonas sp. (SALy), reaction at pH 6.0, 40 °C, removal of non-fucoidan polysaccharides by Ca2+ precipitation, and ethanol-precipitation of crude

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Van Hung, Pham, Nguyen Hai Yen Nhi, Ling Yu Ting, and Nguyen Thi Lan Phi. "Chemical Composition and Biological Activities of Extracts from Pomelo Peel By-Products under Enzyme and Ultrasound-Assisted Extractions." Journal of Chemistry 2020 (February 26, 2020): 1–7. http://dx.doi.org/10.1155/2020/1043251.

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Enzyme-assisted extraction (EAE) and ultrasound-assisted extraction (UAE) were popular methods used to extract bioactive compounds from citrus peels, by-products of fruit processing industry. In this study, the total phenolic content (TPC), total flavonoid content (TFC), naringin and hesperidin contents, and antioxidant and antimicrobial activities of the extracts from pomelo peels using the combined enzyme and ultrasound-assisted extraction (E-UAE) or ultrasound and enzyme-assisted extraction (U-EAE) technique were investigated and compared with those extracted using the EAE and UAE. The opti

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Meghwal, Murlidhar. "Novel Method of Essential oil Extraction from Spices." Nutrition and Food Processing 6, no. 5 (2023): 01–11. http://dx.doi.org/10.31579/2637-8914/141.

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Essential oils have a wide range of industrial and pharmaceutical applications hence different novel technologies are recently being investigated to study the extraction of essential oils, enzyme assisted extraction is one of the recent green technology which is being studied extensively to see its effect on the yield of various components like essential oils and bioactive compounds. This review article summarises the spices in which enzyme-assisted extraction has been employed and its effect on the yield of the essential oil as well as the effect on the quality parameters of the oil, various

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Hooda, Ashish, Dorcus Masih, and Vaibav. "Conventional and Enzyme-Assisted Extraction of Total Flavonoids from Rotten Onion (Allium cepa L.)." Ecology, Environment and Conservation 29, no. 04 (2023): 1643–47. http://dx.doi.org/10.53550/eec.2023.v29i04.030.

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One of the richest sources of dietary flavonoids is the bulb of the onion (Allium cepa L.), which greatly contributes to the daily intake of flavonoids. This research aimed to optimize the yield of Total flavonoids from Onion Solid Waste (OSW). A comparison was drawn between Conventional and Enzyme Assisted extraction methods in terms of the yield of total flavonoids present in OSW extract. The results showed that the highest TFC were obtained at Time/Temp 60 °C for 180 minutes and Pectinase Conc./Time 2g for 120 minutes for Conventional and Enzyme assisted extraction respectively. The yield o

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Sitorus, Randi, Tania Surya Utami, Yuswan Muharam, Rita Arbianti, and Naufal Azrizal Prasetyo. "Ekstraksi Daun Kejibeling (Strobilanthes crispus) dengan Metode Continuous Ultrasound-Assisted Enzymatic Extraction: Pengaruh Temperatur dan Konsentrasi Enzim." Jurnal Teknik Kimia USU 13, no. 2 (2024): 138–45. http://dx.doi.org/10.32734/jtk.v13i2.17151.

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Continuous ultrasound-assisted enzymatic extraction (CUAEE) method has become a promising technique for extracting bioactive compounds from plant materials. In this study, the extraction of kejibeling (Strobilanthes crispus) leaves was carried out using the CUAEE method to optimize the extract yield. The experimental design involved extraction parameters including leaf particle size (d ≤0,177 mm), ethanol concentration (50%), extraction time (180 minutes), and varying parameters such as temperature (30 °C, 40 °C, 50 °C, and 60 °C), as well as cellulase enzyme concentration of 3% and 5%. The re

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Serea, Daniela, Gabriela Râpeanu, Oana Emilia Constantin, Gabriela Elena Bahrim, Nicoleta Stănciuc, and Constantin Croitoru. "Ultrasound and enzymatic assisted extractions of bioactive compounds found in red grape skins Băbească Neagră (Vitis vinifera) variety." Annals of the University Dunarea de Jos of Galati. Fascicle VI - Food Technology 45, no. 1 (2021): 9–25. http://dx.doi.org/10.35219/foodtechnology.2021.1.01.

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The phenolic composition of the skin of the Băbească neagră grapes grown in south-eastern Romania was studied by two extraction techniques, i. e., ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE). This study aimed to analyze the grape skin bioactive compounds and the antioxidant potential using ethanol of three different concentrations 50%, 70%, and 96% and acidified with acetic, citric acid, or hydrochloric acid. This research aimed to maximize the extraction of bioactive compounds from red grape skin by using commercially available oenological enzyme preparations, va

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Liang, Kehong, Qinghua Zhang, and Wei Cong. "Enzyme-Assisted Aqueous Extraction of Lipid from Microalgae." Journal of Agricultural and Food Chemistry 60, no. 47 (2012): 11771–76. http://dx.doi.org/10.1021/jf302836v.

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Domínguez, H. "Enzyme-assisted hexane extraction of soya bean oil." Food Chemistry 54, no. 2 (1995): 223–31. http://dx.doi.org/10.1016/0308-8146(95)00018-e.

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Sharma, Aparna, S. K. Khare, and M. N. Gupta. "Enzyme-assisted aqueous extraction of rice bran oil." Journal of the American Oil Chemists' Society 78, no. 9 (2001): 949–51. http://dx.doi.org/10.1007/s11746-001-0369-x.

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Özkan, Gülay, and Seda Ersus Bilek. "Enzyme-assisted extraction of stabilized chlorophyll from spinach." Food Chemistry 176 (June 2015): 152–57. http://dx.doi.org/10.1016/j.foodchem.2014.12.059.

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Fathollahy, Isa, Akram Pezeshki, and Saeed Mohammad Sorouraddin. "Effect of different extraction methods on trace metal elements content of Persian lime (Citrus latifolia) seed oil." OCL 28 (2021): 44. http://dx.doi.org/10.1051/ocl/2021031.

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The oil of Persian lime (Citrus latifolia) seed was extracted using three methods (cold-pressing, solvent extraction and enzyme-assisted aqueous extraction using Protamex and Celluclast) to compare the effect of extraction method on oil quality through trace metal elements (Fe, Cu, Ni, Co, Pb and As) content. The results demonstrated that extraction method significantly (P < 0.05) influences the trace metal elements content of oil. The reduction pattern of Fe > Cu > Ni > Co > Pb > As was observed in Persian lime seed and extracted oil samples. The highest concentration (mg/kg

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Mac, Hoa Xuan, and Triet Duy Anh Ngo. "ENZYME-ASSISTED AND ULTRASOUND-ASSISTED EXTRACTION OF SAPONIN FROM DANGSHEN (Codonopsis pilosula (Franch) Nannf) ROOTS." Scientific Journal of Tra Vinh University 1, no. 40 (2020): 83–91. http://dx.doi.org/10.35382/18594816.1.40.2020.619.

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This study was carried out to survey the effects of viscozyme L and ultrasound on the efficiency ofsaponin extraction with distilled water from Dangshen (Codonopsis pilosula (Franch) Nannf) roots. Ratio of materialto solvent, hydrolysis time, enzyme concentration, ultrasonic power were investigated. Results showed that the extraction efficiency was significantly increased when viscozyme L and ultrasound wereapplied. The highest content of saponin was 7,75 g/g of dry matter at the ratio of raw materials:to solvent of 1 : 15; hydrolysis time of 1,5 h; viscozyme L concentration of 0,15% (ml/g dry

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Zuorro, Antonio, and Roberto Lavecchia. "Optimization of Enzyme-Assisted Lycopene Extraction from Tomato Processing Waste." Advanced Materials Research 800 (September 2013): 173–76. http://dx.doi.org/10.4028/www.scientific.net/amr.800.173.

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We have investigated the recovery of lycopene from tomato processing waste (TPW) by treating the peel fraction of the waste with pectinolytic and cellulolytic enzyme preparations. To optimize the enzyme-assisted extraction, we used a two-level factorial design with five factors: extraction temperature (T), pretreatment time (P), extraction time (E), enzyme solution-to-solid ratio (R), enzyme load (L). A 7-to 16-fold increase was observed in lycopene recovery, compared to the untreated TPW. From a response surface analysis of the data, a second-degree polynomial equation was developed which pro

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Nova, Paulo, Sara A. Cunha, Ana R. Costa-Pinto, and Ana Maria Gomes. "Chemical and Antioxidant Properties of Solvent and Enzyme-Assisted Extracts of Fucus vesiculosus and Porphyra dioica." Marine Drugs 22, no. 7 (2024): 319. http://dx.doi.org/10.3390/md22070319.

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Extraction strategies impact the efficiency and nature of extracted compounds. This work assessed the chemical composition and antioxidant capacity of ethanolic, hydroethanolic, and aqueous versus enzyme-assisted extracts (isolated or with the sequential use of alcalase®, cellulase®, and viscozyme®) of the macroalgae Fucus vesiculosus (brown, Phaeophyceae) and Porphyra dioica (red, Rhodophyta. For both macroalgae, enzyme-assisted extraction (EAE) was the most efficient process compared to solvent-assisted extraction (SAE), independent of solvent. Fucus vesiculosus extraction yields were higher

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Hien, Tran Thi, and Nguyen Thi Minh. "Enhancing the extraction of pumpkin seed (Cucurbita pepo L) for increasing oil yield and its phytosterol content." Food Science and Applied Biotechnology 4, no. 1 (2021): 6. http://dx.doi.org/10.30721/fsab2021.v4.i1.104.

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Pumpkin seeds (Cucurbita pepo) were harvested in 2018 containing 41.23±1.23% oil; 33.18±1.61% protein; and 1575.97 ±67.79 mg phytosterol in 100g seeds (dried weight). Solvent (hexane) extraction (SE), Ultrasound assisted solvent (hexane) extraction (UAE), Enzyme Alcalase assisted extraction (EAE) and Enzyme Alcalase_Ultrasound assisted extraction (E_UAE) were studied. The oil’s yields and phytosterol in oil obtained were 90.07±0.17% and 1657.6±82.9 mg/100ml oil; 95.46±0.06% and 2017.5±100.1 mg/100ml oil; 89.65±0.11% and 1992.7±99.5 mg/100ml oil; 91.87± 0.03% and 2327.7 ±110.4 mg/100ml oil, res

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Kalcheva-Karadzhova, Krasimira, Vasil Shikov, Kiril Mihalev, Georgi Dobrev, Danka Ludneva, and Nikolai Penov. "Enzyme-Assisted Extraction of Polyphenols From Rose (Rosa Damascena Mill.) Petals." Acta Universitatis Cibiniensis. Series E: Food Technology 18, no. 2 (2014): 65–72. http://dx.doi.org/10.2478/aucft-2014-0015.

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Abstract : The efficiency of enzyme-assisted extraction for the recovery of polyphenols from rose (Rosa damascena Mill.) petals was evaluated performing a simplex centroid experimental design for mixture with three components (pectinolytic, cellulolytic and hemicellulolytic preparation). The ternary enzyme combinations leaded to the highest contents of total polyphenols, reaching 43% higher average value as compared to the control (without enzymatic treatment) sample. Enzymatic treatments also enhanced (9−25%) the extractability of total anthocyanins. The results obtained demonstrate that enzy

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Fu, Xiao-Qian, Gen-Lin Zhang, Long Deng, and Yan-Yan Dang. "Simultaneous extraction and enrichment of polyphenol and lutein from marigold (Tagetes erecta L.) flower by an enzyme-assisted ethanol/ammonium sulfate system." Food & Function 10, no. 1 (2019): 266–76. http://dx.doi.org/10.1039/c8fo01865k.

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Julai, Kanokporn, Pimnapanut Sridonpai, Chitraporn Ngampeerapong, et al. "Effects of Extraction and Evaporation Methods on Physico-Chemical, Functional, and Nutritional Properties of Syrups from Barhi Dates (Phoenix dactylifera L.)." Foods 12, no. 6 (2023): 1268. http://dx.doi.org/10.3390/foods12061268.

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Date fruits (Phoenix dactylifera L.) are rich in sugar and also contain a substantial amount of phenolic compounds. Therefore, date fruits can be used to produce an alternative sweetener, having lower glycemic index than sucrose. This study investigated the effects of extraction and evaporation methods on various properties of the syrups prepared from Barhi dates. Extraction of date juice with the aid of pectinase or cellulase significantly enhanced the production yield, total phenolic content, and antioxidant activities determined by Ferric Reducing Antioxidant Power and Oxygen Radical Absorb

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Nguyen Tram Anh, Mai, Pham Van Hung, and Nguyen Thi Lan Phi. "Optimized Conditions for Flavonoid Extraction from Pomelo Peel Byproducts under Enzyme- and Ultrasound-Assisted Extraction Using Response Surface Methodology." Journal of Food Quality 2021 (January 15, 2021): 1–10. http://dx.doi.org/10.1155/2021/6666381.

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Pomelo (Citrus maxima (Burm.) Merrill) peel, which has been considered as a byproduct, is a potential source of flavonoids. In this study, the extraction conditions of total flavonoids from peels of Da Xanh pomelo were optimized using the response surface methodology with the Box–Behnken design. The combination of two novel extraction methods, including enzyme-assisted and ultrasound-assisted extractions, was used for the optimization. The results indicated that the optimal extraction condition was a liquid-to-solid ratio of 142.99 mL/g, enzyme concentration of 3.45%, and the time of 65.23 min

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Nguyen, Thi Kim Ngan, Thi Thuy Linh Ngo, Do Dat Tran, et al. "Effects of extraction technology on bioactivities from polysaccharide-richGanoderma lucidum." Ministry of Science and Technology, Vietnam 64, no. 11 (2022): 32–37. http://dx.doi.org/10.31276/vjst.64(11).32-37.

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In this study, the polysaccharide-riched extract from Ganoderma lucidum was obtained by different extraction methods including hot water extraction (HWE), ultrasonic-assisted hot water extraction (UHWE), enzyme-assisted extraction (EAE), and ultrasonic-assisted enzyme extraction (UAEE). The total polysaccharide content in UAEE (3.721±0.134%) was higher than HWE (1.783±0.156%), UHWE (1.886±0.148%), and EAE (2.133±0.139%). The G. lucidum extract was then evaluated for antioxidant activity by 2,2-diphenyl-1-1picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The antibacte

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Šalić, Anita, Lina Šepić, Iva Turkalj, Bruno Zelić, and Dunja Šamec. "Comparative Analysis of Enzyme-, Ultrasound-, Mechanical-, and Chemical-Assisted Extraction of Biflavonoids from Ginkgo Leaves." Processes 12, no. 5 (2024): 982. http://dx.doi.org/10.3390/pr12050982.

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The biflavonoid extraction from ginkgo (Ginkgo biloba L.) leaves using solvent-based extraction with 70% ethanol, alone and in combination with enzyme-assisted, ultrasound-assisted, mechanical-assisted, and chemically assisted methods was investigated and the influence of extraction duration was explored. The total content of polyphenols, flavonoids, and phenolic acids in the extracts was determined spectrophotometrically, while individual biflavonoids were identified and quantified using HPLC-DAD. Amentoflavone, bilobetin, ginkgetin, isoginkgetin, and sciadopitysin were identified in all our

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Haque, Shaikh Manirul, Abuzar Kabir, Elaref Ratemi, et al. "Greener Pectin Extraction Techniques: Applications and Challenges." Separations 12, no. 3 (2025): 65. https://doi.org/10.3390/separations12030065.

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Pectin is a complex and versatile polysaccharide crucial for various industries. It functions as a thickener, gelling agent, emulsifier, and low-calorie food. Its anti-inflammatory and immunomodulatory properties have attracted biomedical interest, while its biodegradability and biocompatibility make it valuable for biomaterial applications. The effectiveness of these applications depends on the quality of pectin extraction procedures. While traditional extraction methods exist, green methodologies and alternative techniques have improved pectin’s physicochemical properties—a significant advan

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Mattusch, Jürgen, Maria Cimpean, and Rainer Wennrich. "Enzyme-assisted extraction of arsenic species from plant material." International Journal of Environmental Analytical Chemistry 86, no. 9 (2006): 629–40. http://dx.doi.org/10.1080/03067310600557489.

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Chung, Soo-Hyun, Dojin Ryu, Eun-Kyung Kim, and Lloyd B. Bullerman. "Enzyme-Assisted Extraction of Moniliformin from Extruded Corn Grits." Journal of Agricultural and Food Chemistry 53, no. 13 (2005): 5074–78. http://dx.doi.org/10.1021/jf0580014.

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Zuorro, Antonio, Gianluca Maffei, and Roberto Lavecchia. "Optimization of enzyme-assisted lipid extraction from Nannochloropsis microalgae." Journal of the Taiwan Institute of Chemical Engineers 67 (October 2016): 106–14. http://dx.doi.org/10.1016/j.jtice.2016.08.016.

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Zuorro, Antonio, Marcello Fidaleo, and Roberto Lavecchia. "Enzyme-assisted extraction of lycopene from tomato processing waste." Enzyme and Microbial Technology 49, no. 6-7 (2011): 567–73. http://dx.doi.org/10.1016/j.enzmictec.2011.04.020.

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