Log in

Relevant bibliographies by topics / Enzyme-degradable / Journal articles

To see the other types of publications on this topic, follow the link: Enzyme-degradable.

Journal articles on the topic 'Enzyme-degradable'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Enzyme-degradable.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Chen, Ginger, Shadi Taghavi, Dale Marecak, and Brian G. Amsden. "Tough and Enzyme-Degradable Hydrogels." Macromolecular Materials and Engineering 303, no. 1 (July 24, 2017): 1700162. http://dx.doi.org/10.1002/mame.201700162.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Rypáček, František, and Václav Škarda. "Self-Degradable Hydrogel with Covalently Bound Proteolytic Enzyme." Collection of Czechoslovak Chemical Communications 60, no. 11 (1995): 1986–94. http://dx.doi.org/10.1135/cccc19951986.

Full text

Abstract:

Biodegradable hydrogel as a self-degradable system for controlled release of biologically active macromolecules was prepared from the biodegradable poly[N5-(2-hydroxyethyl)-L-glutamine-stat -N5-(2-methacryloyloxyethyl)-L-glutamine -stat-L-glutamic acid], to which a proteolytic enzyme, papain, was covalently bound. The polymer-enzyme conjugate was crosslinked by radical copolymerization with acrylamide. Significantly improved thermal stability and increased pH optimum of the catalytic activity of conjugated papain was observed. Self-degradation of the hydrogel by the action of encapsulated papain could be triggered by the addition of a low-molecular-weight enzyme activator, such as dithioerythritol.

APA, Harvard, Vancouver, ISO, and other styles

3

Murase, S. K., L. P. Lv, A. Kaltbeitzel, K. Landfester, L. J. del Valle, R. Katsarava, J. Puiggali, and D. Crespy. "Amino acid-based poly(ester amide) nanofibers for tailored enzymatic degradation prepared by miniemulsion-electrospinning." RSC Advances 5, no. 68 (2015): 55006–14. http://dx.doi.org/10.1039/c5ra06267e.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Lévesque, Stéphane G., and Molly S. Shoichet. "Synthesis of Enzyme-Degradable, Peptide-Cross-Linked Dextran Hydrogels." Bioconjugate Chemistry 18, no. 3 (May 2007): 874–85. http://dx.doi.org/10.1021/bc0602127.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Bacinello, Daniel, Elisabeth Garanger, Daniel Taton, Kam Chiu Tam, and Sébastien Lecommandoux. "Enzyme-Degradable Self-Assembled Nanostructures from Polymer–Peptide Hybrids." Biomacromolecules 15, no. 5 (April 10, 2014): 1882–88. http://dx.doi.org/10.1021/bm500296n.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Whang, Minji, Hyeonji Yu, and Jungwook Kim. "Superabsorbent Polymer Network Degradable by a Human Urinary Enzyme." Polymers 13, no. 6 (March 17, 2021): 929. http://dx.doi.org/10.3390/polym13060929.

Full text

Abstract:

Owing to its superior water absorption capacity, superabsorbent polymer (SAP) based on a poly (acrylic acid) network is extensively used in industrial products such as diapers, wound dressing, or surgical pads. However, because SAP does not degrade naturally, a massive amount of non-degradable waste is discarded daily, posing serious environmental problems. Considering that diapers are the most widely used end-product of SAP, we created one that is degradable by a human urinary enzyme. We chose three enzyme candidates, all of which have substrates that were modified with polymerizable groups to be examined for cleavable crosslinkers of SAP. We found that the urokinase-type plasminogen activator (uPA) substrate, end-modified with acrylamide groups at sufficient distances from the enzymatic cleavage site, can be successfully used as a cleavable crosslinker of SAP. The resulting SAP slowly degraded over several days in the aqueous solution containing uPA at a physiological concentration found in human urine and became shapeless in ~30 days.

APA, Harvard, Vancouver, ISO, and other styles

7

Donskyi, Ievgen S., Ying Chen, Philip Nickl, Guy Guday, Haishi Qiao, Katharina Achazi, Andreas Lippitz, et al. "Self-degrading graphene sheets for tumor therapy." Nanoscale 12, no. 26 (2020): 14222–29. http://dx.doi.org/10.1039/d0nr02159h.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Tsao, Nadia H., and Elizabeth A. H. Hall. "Enzyme-Degradable Hybrid Polymer/Silica Microbubbles as Ultrasound Contrast Agents." Langmuir 32, no. 25 (June 16, 2016): 6534–43. http://dx.doi.org/10.1021/acs.langmuir.6b01075.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Wu, Haiyan, Huifeng Wang, Fang Cheng, Fujian Xu, and Gang Cheng. "Synthesis and characterization of an enzyme-degradable zwitterionic dextran hydrogel." RSC Advances 6, no. 37 (2016): 30862–66. http://dx.doi.org/10.1039/c6ra00550k.

Full text

Abstract:

A matrix metalloproteinase peptide cross-linked dextran hydrogel was synthesized. Dextran was modified with carboxybetaine to resist nonspecific protein adsorption and cell attachment. The degradable hydrogel is a good candidate for soft tissue engineering applications.

APA, Harvard, Vancouver, ISO, and other styles

10

Shalaby, Waleed S. W., Garnet E. Peck, and Kinam Park. "Release of dextromethorphan hydrobromide from freeze-dried enzyme-degradable hydrogels." Journal of Controlled Release 16, no. 3 (August 1991): 355–63. http://dx.doi.org/10.1016/0168-3659(91)90013-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Radchicov, V., V. Tzai, A. Kot, T. Sapsaleva, G. Besarab, S. Razumovskyi, and O. Shulko. "Rumen cannulation of young cattle depending on protein diet." Tehnologìâ virobnictva ì pererobki produktìv tvarinnictva, no. 2(150) (December 17, 2019): 93–104. http://dx.doi.org/10.33245/2310-9289-2019-150-2-93-104.

Full text

Abstract:

An experiment has been carried to determine the degradable and non-degradable protein content in grass and concentrated feed and its influence on operated sire rumen cannulation of black-and-white breed (with body weight of 120–160 kg). The grass chemical composition and concentrated feed research shows that there is a wide range of crude protein content and its degradable and non-degradable fractions. The level of crude protein in concentrated feed varies from 96 g (barley grain) to 380–383 g (extruded lupine grain and rapeseed meal). The amount of degradable protein is 81 (barley grain), 303 g (rapeseed meal). Degradability of crude protein ranges from 57 % (extruded rapeseed) to 84–86 % (barley and wheat grain). The crude protein content in the presented samples of grass feed is 27 (corn silage) – 93 g (cereal hay), degradable protein – from 17–19 (cereal hay and corn silage), up to 38 g (mixed grass), non-degradable – 8 (corn silage, 76 g (cereal hay). The degradable and non-degradable protein ration was 2:7. The crude protein increase in summer and winter calve diet up to 70 % contributes a lower accumulation of ammonia in the rumen fluid (by 19.6–20.6 % ) and activation of VFA synthesis (by 16.5–18.2). It also contributes the increase of the ciliate number (by15,7–15,9), total and protein nitrogen (by 7.2–7.4 and 8.0–12.3 %). Feeding on protein degradability (of 65–60 %) lets the rumen metabolism processes slow down, reduce the microbiota enzyme activity, the protein nitrogen proportion and the ammonia level increasing. According to the analysis results of economic diet indices with different protein fractional composition it has been determined that the ration use with protein degradability of 70 %, and metabolizable energy costs – by 4.0–5.0 % are economically reasonable in summer and winter periods. Key words: concentrated feeds, grain, degradable protein, non-degradable protein, calves.

APA, Harvard, Vancouver, ISO, and other styles

12

Yang, Cheng, Xin Shu, Shujuan Hou, and Yiqi Mao. "Chemo-thermomechanical behaviors of Enzyme-degradable shape memory composite and its heat-enzyme triggered shape memory properties." Computational Materials Science 193 (June 2021): 110382. http://dx.doi.org/10.1016/j.commatsci.2021.110382.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Wachiralarpphaithoon, Chookaet, Yasuhiko Iwasaki, and Kazunari Akiyoshi. "Enzyme-degradable phosphorylcholine porous hydrogels cross-linked with polyphosphoesters for cell matrices." Biomaterials 28, no. 6 (February 2007): 984–93. http://dx.doi.org/10.1016/j.biomaterials.2006.10.024.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Castelletto, V., R. J. Gouveia, C. J. Connon, I. W. Hamley, J. Seitsonen, J. Ruokolainen, E. Longo, and G. Siligardi. "Influence of elastase on alanine-rich peptide hydrogels." Biomater. Sci. 2, no. 6 (2014): 867–74. http://dx.doi.org/10.1039/c4bm00001c.

Full text

Abstract:

The self-assembly of the alanine-rich amphiphilic peptides Lys(Ala)₆Lys (KA₆K) and Lys(Ala)₆Glu (KA₆E) with homotelechelic or heterotelechelic charged termini respectively has been investigated in aqueous solution. The latter forms enzyme-degradable hydrogels.

APA, Harvard, Vancouver, ISO, and other styles

15

Moss, Jason A., Shula Stokols, Mark S. Hixon, Fawn T. Ashley, Jason Y. Chang, and Kim D. Janda. "Solid-Phase Synthesis and Kinetic Characterization of Fluorogenic Enzyme-Degradable Hydrogel Cross-linkers." Biomacromolecules 7, no. 4 (April 2006): 1011–16. http://dx.doi.org/10.1021/bm051001s.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Li Volsi, Anna, Francesca Tallia, Haffsah Iqbal, Theoni K. Georgiou, and Julian R. Jones. "Enzyme degradable star polymethacrylate/silica hybrid inks for 3D printing of tissue scaffolds." Materials Advances 1, no. 9 (2020): 3189–99. http://dx.doi.org/10.1039/d0ma00674b.

Full text

Abstract:

We report the first enzyme cleavable inorganic–organic hybrid “inks” that can be 3D printed as scaffolds for bone regeneration and investigate the effect of star polymer architecture on their properties.

APA, Harvard, Vancouver, ISO, and other styles

17

Thornton, Paul D., Shah M. Reduwan Billah, and Neil R. Cameron. "Enzyme-Degradable Self-Assembled Hydrogels From Polyalanine-Modified Poly(ethylene glycol) Star Polymers." Macromolecular Rapid Communications 34, no. 3 (January 3, 2013): 257–62. http://dx.doi.org/10.1002/marc.201200649.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Wen, Zhi Guo, Chun Yan Li, Cong Cong Hu, Jian Hua Huang, and Sheng Xiong Dong. "Study on the Degradation in Vitro of Ciprofloxacin-Polyurethane Materials." Advanced Materials Research 239-242 (May 2011): 963–67. http://dx.doi.org/10.4028/www.scientific.net/amr.239-242.963.

Full text

Abstract:

Bacterial induced infection is a major complication associated with the use of medical implants. Degradable antibacterial ciprofloxacin-polyurethanes (CFPU) have been synthesized in attempts to address this problem. It is supposed that the material may be sensitively hydrolyzed by inflammatory enzyme, cholesterol esterase (CE), and the drug could be released according to the state of infection. The enzyme biodegradation experiments showed an extra release of ciprofloxacin when CFPU was incubated by enzyme solutions than by phosphate-buffer saline (PBS). Results showed that the drug release was enhanced as the concentration of the enzyme increased. The antimicrobial activities of degradation solutions were tested by broth dilution assay. The enzyme degradation solutions exhibited an ability to kill bacteria. The cell cytotoxicity assay indicated that the degradation products were hypotoxicity to human beings according to the cytotoxicity grade of United States Pharmacopoeia (USP).

APA, Harvard, Vancouver, ISO, and other styles

19

Keen, Imelda, Lynette Lambert, Traian V. Chirila, Stefan M. Paterson, and Andrew K. Whittaker. "Degradable Hydrogels for Tissue Engineering – Part I: Synthesis by RAFT Polymerization and Characterization of PHEMA Containing Enzymatically Degradable Crosslinks." Journal of Biomimetics, Biomaterials and Tissue Engineering 6 (September 2010): 67–85. http://dx.doi.org/10.4028/www.scientific.net/jbbte.6.67.

Full text

Abstract:

A nonapeptide, which is sensitive to enzymatic digestion by collagenase, was modified by the covalent attachment of an acrylamido group at the terminal positions. The functionalized peptide was used as a crosslinking agent during polymerization of 2-hydroxyethyl methacrylate (HEMA). Reversible addition-fragmentation chain transfer (RAFT) method was used to obtain a polymer (PHEMA) with an average theoretical molecular weight of 4000 Da, containing enzymatically labile peptide crosslinks. The functionalized peptide was analyzed in detail by 1H and 13C nuclear magnetic resonance (NMR) spectrometry. The polymerization reaction was monitored by near infrared spectrometry, while the resulting polymer was analyzed by size exclusion chromatography and solid NMR spectrometry. The peptide-crosslinked PHEMA was subjected to an in-vitro degradation assay in the presence of collagenase. At the highest concentration of enzyme used in the study, a weight loss of 35% was recorded after 60 days of incubation in the collagenolytic medium. This suggests that crosslinking with enzymatically degradable peptides is a valid method for inducing biodegradability in polymers that otherwise are not degradable.

APA, Harvard, Vancouver, ISO, and other styles

20

Zhou, Jianhua, Dong Choon Hyun, Hang Liu, Hongkai Wu, and Younan Xia. "Protein Capsules with Cross-Linked, Semipermeable, and Enzyme-Degradable Surface Barriers for Controlled Release." Macromolecular Rapid Communications 35, no. 16 (June 24, 2014): 1436–42. http://dx.doi.org/10.1002/marc.201400201.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Taghavi, Shadi, Amanda Brissenden, and Brian G. Amsden. "High modulus, enzyme-degradable poly(trimethylene carbonate)–peptide biohybrid networks formed from triblock prepolymers." Journal of Materials Chemistry B 7, no. 17 (2019): 2819–28. http://dx.doi.org/10.1039/c8tb02195c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Su, Chia-Wei, San-Yuan Chen, and Dean-Mo Liu. "Polysaccharide-lecithin reverse micelles with enzyme-degradable triglyceride shell for overcoming tumor multidrug resistance." Chemical Communications 49, no. 36 (2013): 3772. http://dx.doi.org/10.1039/c3cc40836a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Zhou, Jianhua, Sung-Wook Choi, Haijun Yu, Younan Xia, and Hongkai Wu. "Uniform protein microcapsules with semi-permeable, and enzyme-degradable walls for drug delivery applications." Journal of Controlled Release 172, no. 1 (November 2013): e106. http://dx.doi.org/10.1016/j.jconrel.2013.08.259.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Ahmed, Marya, and Ravin Narain. "Intracellular Delivery of DNA and Enzyme in Active Form Using Degradable Carbohydrate-Based Nanogels." Molecular Pharmaceutics 9, no. 11 (September 25, 2012): 3160–70. http://dx.doi.org/10.1021/mp300255p.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Cai, Ji Hua, Sui Gu, and Hao Liu. "Gel Breaking Affecting Factors Analysis on Polymer Bio-Degradation." Advanced Materials Research 550-553 (July 2012): 1369–73. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.1369.

Full text

Abstract:

Recent years have witnessed a renewed interest in the development of coalbed methane (CBM) reservoirs. However, horizontal drilling in unconsolidated and soft coal seam for CBM drainage and exploitation often results in wellbore instability. This paper proposed degradable drilling fluid in CBM horizontal drilling which not only can maintain wellbore stability, but also minimize formation damages caused by drilling fluid. Its factors of gel breaking ratio were systematically studied from the aspects as substrate concentration, pH, temperature, enzyme concentration and reaction time. We found that in a condition when enzyme concentration is abundant, increasing of substrate concentration would result in higher gel breaking ratio. Furthermore, nearly neutral pH (pH≈7), moderate temperature (40°C~60°C), enzyme with higher concentration and longer action time (more than 3 hours) could enhance gel breaking ratio.

APA, Harvard, Vancouver, ISO, and other styles

26

Oh, Hyun Deok, Min Seo Cho, Joong-Su Kim, Min-Soo Kim, Chul Ho Kim, and Ji Young Kang. "Identification and Characterization of a Cocoon Degradable Enzyme from the Isolated Strain Bacillus subtilis Bs5C." Biotechnology and Bioprocess Engineering 25, no. 3 (June 2020): 442–49. http://dx.doi.org/10.1007/s12257-019-0399-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Engels, F. M., and N. A. Schalk. "Ultrastructural investigations of enzyme treated cell wall material from industrial by-products." Netherlands Journal of Agricultural Science 40, no. 2 (June 1, 1992): 137–46. http://dx.doi.org/10.18174/njas.v40i2.16520.

Full text

Abstract:

Ultrastructural investigations of glycanase treatments of cell wall material from industrial by-products was carried out with the enzyme-thiocarbohydrazide-silver protein technique (ETAg). Driselase, gamanase and an experimental enzyme preparation with a broad spectra of hemicellulase, cellulase and pectolytic activities were used. Carbohydrate hydrolysis was found in cell walls and cell contents in 1-2 cell layers at the surface of 0.2-mm plant particles. Enzyme activity was detected to some extent inside unlignified and lignified cell walls. The absence of any reaction in some sublayers of the cell wall was indicative for the absence of polysaccharide substrate or a very tied interaction of cell wall components preventing enzymic activity or the absence of typical enzymes in crude preparations. It was concluded that the ETAg-technique can be useful to provide information about structural limitations of poorly degradable plant materials. (Abstract retrieved from CAB Abstracts by CABI’s permission)

APA, Harvard, Vancouver, ISO, and other styles

28

Sher, Hassan, Muhammad Faheem, Abdul Ghani, Rashid Mehmood, Hamza Rehman, and Syed A. I. Bokhari. "OPTIMIZATION OF CELLULASE ENZYME PRODUCTION FROM Aspergillus oryzae FOR INDUSTRIAL APPLICATIONS." World Journal of Biology and Biotechnology 2, no. 2 (August 15, 2017): 155. http://dx.doi.org/10.33865/wjb.002.02.0088.

Full text

Abstract:

Cellulases are the hydrolytic group of enzymes, responsible for release of sugars in the bioconversion of the cellulosic biomass into a variety of value added industrial products. Fungal isolated cellulases are well studied and playing a significant role in various industrial processes. Enzymatic depolymerisation of cellulosic material has been done by the various fungal isolated enzymes. In the present study, the cultivation conditions for cellulase production from Aspergillus species were optimized. Optimization of scarification conditions such as time course, inoculum size, carbon source and concentration, nitrogen source, various pH levels were performed for the production of extracellular carboxymethyl cellulase and endoglucanase enzyme. The result exhibited, 15 % inoculums size, corncobs 2 % concentration, Urea and medium pH 7 at 30oC supported high yield of carboxymethyl cellulase (38.80 U/ml/min) and exoglucanase enzyme (10.94 U/ml/min) through a submerged fermentation (SmF). In future biotechnological applications in cellulase enzyme production attain a vital role to obtain high degradable yield.

APA, Harvard, Vancouver, ISO, and other styles

29

Zhang, Wendi, Qiang Cheng, Shutao Guo, Daoshu Lin, Pingsheng Huang, Juan Liu, Tuo Wei, et al. "Gene transfection efficacy and biocompatibility of polycation/DNA complexes coated with enzyme degradable PEGylated hyaluronic acid." Biomaterials 34, no. 27 (September 2013): 6495–503. http://dx.doi.org/10.1016/j.biomaterials.2013.04.030.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Wedegärtner, David, and Oliver I. Strube. "Site-Specific Addressing of Particles and Coatings via Enzyme-Mediated Destabilization." Catalysts 9, no. 4 (April 12, 2019): 354. http://dx.doi.org/10.3390/catal9040354.

Full text

Abstract:

Enzyme mediated addressing (EMA) is a highly specific and easy-to-apply technology for direction and deposition of particles and coatings on surfaces. Key feature of this process is an enzymatic reaction in direct proximity to the surface, which induces the deposition. The technique has previously shown great success in the handling of biological particles. In this study, addressing of non-biological nanoparticles, in particular plastics and metals, is presented. The respective particles are stabilized by an amphiphilic, enzyme-degradable block copolymer, consisting of poly(ethylene glycol) and poly(caprolactone). After contact with the enzyme pseudomonas lipase, the particles are destabilized, due to the loss of the hydrophilic part of the block copolymer. The lipase is therefore immobilized on glass supports. Immobilization is performed via adsorption or covalent bonding to epoxide groups. All deposition experiments show that addressing of individual particles occurs precisely within the predefined areas of enzyme activity. Depending on the material and reaction conditions, intact nanoparticles or coatings from such can be gained. The quintessence of the study is the indifference of the EMA regarding particle materials. From this rationale, the technique offers near unlimited materials compatibility within a precise, easy-to-apply, and upscalable process.

APA, Harvard, Vancouver, ISO, and other styles

31

Subrahmaniyan, K., and Weijun Zhou. "Soil Temperature Associated with Degradable, Non-Degradable Plastic and Organic Mulches and Their Effect on Biomass Production, Enzyme Activities and Seed Yield of Winter Rapeseed (Brassica napus L.)." Journal of Sustainable Agriculture 32, no. 4 (October 15, 2008): 611–27. http://dx.doi.org/10.1080/10440040802394927.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Connon, Che J., and Ricardo M. Gouveia. "Autogenous Biofabrication of Nativelike, Scaffold-Free Human Skin Equivalents Using a Smart, Enzyme-Degradable Tissue Templating Coating." ACS Applied Bio Materials 2, no. 2 (December 31, 2018): 838–47. http://dx.doi.org/10.1021/acsabm.8b00685.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Yao, Mengqun, Yinchu Ma, Hang Liu, Malik Ihsanullah Khan, Song Shen, Shuya Li, Yangyang Zhao, et al. "Enzyme Degradable Hyperbranched Polyphosphoester Micellar Nanomedicines for NIR Imaging-Guided Chemo-Photothermal Therapy of Drug-Resistant Cancers." Biomacromolecules 19, no. 4 (March 7, 2018): 1130–41. http://dx.doi.org/10.1021/acs.biomac.7b01793.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Fude, Cui, Yang Lei, Jin Jie, Piao Hongze, Lin Wenhui, and Cun Dongmei. "Preparation and In Vitro Evaluation of pH, Time-Based and Enzyme-Degradable Pellets for Colonic Drug Delivery." Drug Development and Industrial Pharmacy 33, no. 9 (January 2007): 999–1007. http://dx.doi.org/10.1080/03639040601150393.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Taghavi, Shadi, and Brian G. Amsden. "In vivo degradation behavior of enzyme-degradable poly(trimethylene carbonate)-based biohybrid networks of varying water content." Biomedical Materials 15, no. 2 (February 17, 2020): 025001. http://dx.doi.org/10.1088/1748-605x/ab62ff.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Elkhodairy, Kadria A., Hanna A. Elsaghir, and Amal M. Al-Subayiel. "Formulation of Indomethacin Colon Targeted Delivery Systems Using Polysaccharides as Carriers by Applying Liquisolid Technique." BioMed Research International 2014 (2014): 1–17. http://dx.doi.org/10.1155/2014/704362.

Full text

Abstract:

The present study aimed at the formulation of matrix tablets for colon-specific drug delivery (CSDD) system of indomethacin (IDM) by applying liquisolid (LS) technique. A CSDD system based on time-dependent polymethacrylates and enzyme degradable polysaccharides was established. Eudragit RL 100 (E-RL 100) was employed as time-dependent polymer, whereas bacterial degradable polysaccharides were presented as LS systems loaded with the drug. Indomethacin-loaded LS systems were prepared using different polysaccharides, namely, guar gum (GG), pectin (PEC), and chitosan (CH), as carriers separately or in mixtures of different ratios of 1 : 3, 1 : 1, and 3 : 1. Liquisolid systems that displayed promising results concerning drug release rate in both pH 1.2 and pH 6.8 were compressed into tablets after the addition of the calculated amount of E-RL 100 and lubrication with magnesium stearate and talc in the ratio of 1 : 9. It was found that E-RL 100 improved the flowability and compressibility of all LS formulations. The release data revealed that all formulations succeeded to sustain drug release over a period of 24 hours. Stability study indicated that PEC-based LS system as well as its matrix tablets was stable over the period of storage (one year) and could provide a minimum shelf life of two years.

APA, Harvard, Vancouver, ISO, and other styles

37

Herting, Scott M., Mary Beth B. Monroe, Andrew C. Weems, Sam T. Briggs, Grace K. Fletcher, Samuel E. Blair, Christopher J. Hatch, and Duncan J. Maitland. "In vitro cytocompatibility testing of oxidative degradation products." Journal of Bioactive and Compatible Polymers 36, no. 3 (March 18, 2021): 197–211. http://dx.doi.org/10.1177/08839115211003115.

Full text

Abstract:

Implantable medical devices must undergo thorough evaluation to ensure safety and efficacy before use in humans. If a device is designed to degrade, it is critical to understand the rate of degradation and the degradation products that will be released. Oxidative degradation is typically modeled in vitro by immersing materials or devices in hydrogen peroxide, which can limit further analysis of degradation products in many cases. Here we demonstrate a novel approach for testing the cytocompatibility of degradation products for oxidatively-degradable biomaterials where the materials are exposed to hydrogen peroxide, and then catalase enzyme is used to convert the hydrogen peroxide to water and oxygen so that the resulting aqueous solution can be added to cell culture media. To validate our results, expected degradation products are also synthesized then added to cell culture media. We used these methods to evaluate the cytocompatibility of degradation products from an oxidatively-degradable shape memory polyurethane designed in our lab and found that the degradation of these polymers is unlikely to cause a cytotoxic response in vivo based on the guidance provided by ISO 10993-5. These methods may also be applicable to other biocompatibility tests such as tests for mutagenicity or systemic toxicity, and evaluations of cell proliferation, migration, or gene and protein expression.

APA, Harvard, Vancouver, ISO, and other styles

38

Zhang, Shan, Zhitao Zhou, Junjie Zhong, Zhifeng Shi, Ying Mao, and Tiger H. Tao. "Body‐Integrated, Enzyme‐Triggered Degradable, Silk‐Based Mechanical Sensors for Customized Health/Fitness Monitoring and In Situ Treatment." Advanced Science 7, no. 13 (May 11, 2020): 1903802. http://dx.doi.org/10.1002/advs.201903802.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Chaudhry, A. S. "Estimation of in vitro degradation of dietary proteins in ruminants by using enzymes." BSAP Occasional Publication 22 (1998): 285–87. http://dx.doi.org/10.1017/s0263967x00032882.

Full text

Abstract:

Protein degradation in the rumen determines the supply of dietary nitrogen (N) to microbes and undegraded amino acids for direct absorption. The current systems of feeding ruminants identify rumen degradable protein (RDP) from undegraded protein (UDP) which is digestible post ruminally (Agricultural and Food Research Council (AFRC), 1992; Huntington and Givens 1995). The in sacco method has been useful to estimate RDP, UDP and rate of degradation of proteins in ruminants. However, its use is constrained by the fact that it requires surgically modified animals, is laborious, inconsistent and does not differentiate between proteins that disappear from the bag or which are digested by microbes. It is therefore important to develop rapid, robust and reproducible alternatives (in vitro) to simulate ruminal digestion of proteins. Chaudhry (1998) has shown a potential in Streptomyces griseus protease (enzyme) to estimate ruminal digestion in vitro given its consistently high proteolytic activity with purified proteins. This study examined the validity of this enzyme to estimate degradability of food proteins.Buffer, enzyme and foods A potassium phosphate buffer (buffer) with a pH 6-8 was used to prepare protein suspensions and a solution of the protease (P5147, SIGMA). Samples of sunflower meal (SF), maize-gluten meal (MG), distillers’ dark grains (DG) and field beans (FB) were dried and ground through 1 or 4 mm sieves for in vitro enzyme and in sacco studies respectively.

APA, Harvard, Vancouver, ISO, and other styles

40

Adamski, J., B. Husen, H. H. Thole, U. Groeschel-Stewart, and P. W. Jungblut. "Linkage of 17 β-oestradiol dehydrogenase to actin by ɛ-(γ-glutamyl)-lysine in porcine endometrial cells." Biochemical Journal 296, no. 3 (December 15, 1993): 797–802. http://dx.doi.org/10.1042/bj2960797.

Full text

Abstract:

We report on the discovery of interactions of porcine endometrial 17 beta-oestradiol dehydrogenase with actin. The 17 beta-oestradiol dehydrogenase of porcine uteri is an essentially unidirectional enzyme compounded in specialized organelles. The enzyme activity in Brij 35 extracts of the particulate fraction of epithelial cells sedimenting between 1800 and 11,000 g(av). was collected by immunoadsorption and eluted at low pH. The eluate contained three proteins of 32, 45 and 80 kDa as shown by SDS/PAGE and silver staining. They were identified by amino acid sequencing and immunotyping as oestradiol dehydrogenase (32 kDa), actin (45 kDa) and a covalent dehydrogenase-actin complex (80 kDa). Disulphides, aldimines, periodate-degradable bonds and hydrophobic interactions were excluded as linkages in the 80 kDa protein. The epsilon-(gamma-glutamyl)-lysine nature of the covalent cross-link was recognized by narrow-bore h.p.l.c. analysis of enzymic digests of electro-eluted 80 kDa material. An involvement of the actin anchor in positioning of the oestradiol dehydrogenase-containing organelles according to metabolic requirements is discussed.

APA, Harvard, Vancouver, ISO, and other styles

41

Haas, Simon, Nicole Hain, Mohammad Raoufi, Stephan Handschuh-Wang, Tao Wang, Xin Jiang, and Holger Schönherr. "Enzyme Degradable Polymersomes from Hyaluronic Acid-block-poly(ε-caprolactone) Copolymers for the Detection of Enzymes of Pathogenic Bacteria." Biomacromolecules 16, no. 3 (February 19, 2015): 832–41. http://dx.doi.org/10.1021/bm501729h.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Kim, In-Sook, and In-Joon Oh. "Drug release from the enzyme-degradable and pH-sensitive hydrogel composed of glycidyl methacrylate dextran and poly(acrylic acid)." Archives of Pharmacal Research 28, no. 8 (August 2005): 983–87. http://dx.doi.org/10.1007/bf02973887.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Saha, Pradip, MR Khan, TK Deb, S. Majumdar, F. Alam, and NC Sarker. "Enzymatic Hydrolysis of Rice Straw to Fermentable Sugar: Kinetic Study." Journal of Chemical Engineering 27, no. 2 (January 28, 2014): 20–24. http://dx.doi.org/10.3329/jce.v27i2.17778.

Full text

Abstract:

As the gradual up-growing trend of industrialization and urbanization leading steady increment of demand of energy; eco-friendly, bio degradable, cost competitive and promising source of energy with high sustainability is toughly needed for the new era of modern world. Hydrolysis of cellulose by cellulase enzymes is a vital candidate for this option. It is a solid-liquid heterogeneous reaction; strongly affected by the non-reaction resistances caused most notably by the crystalline structure; reaction environment parameters as temperature, pH, characteristics of enzyme, cell & substrate loading and hence must have to be defined for specific enzyme-substrate amalgamation. In this present investigation, glucose was produced from rice straw using cellulytic enzyme pseudomonas sp., isolated from municipal solid waste. Glucose yield was found to increase as the rice straw particle size decreased from 0.5 mm to 45 ?m, while the optimal temperature and pH were found within the range of 30°C and 7.0 respectively. The concentration and rate of glucose production was observed to depend on pretreatment of rice straw, substrate concentration and enzyme loading. A kinetic model rate expression has been developed for such a process based on the Michaelis Mentens and Line weaverBurk approach. Comparison between the experimental data and those predicted from the rate model indicate good agreement with a mean absolute deviation of about 0.304916. DOI: http://dx.doi.org/10.3329/jce.v27i2.17778 Journal of Chemical Engineering, IEB Vol. ChE. 27, No. 2, December 2012: 20-24

APA, Harvard, Vancouver, ISO, and other styles

44

Amiji, Mansoor, Rakhee Tailor, Mai-Ki Ly, and Joseph Goreham. "Gelatin-Poly(Ethylene Oxide) Semi-interpenetrating Polymer Network with pH-Sensitive Swelling and Enzyme-Degradable Properties for Oral Drug Delivery." Drug Development and Industrial Pharmacy 23, no. 6 (January 1997): 575–82. http://dx.doi.org/10.3109/03639049709149822.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Ghaffar, A., G. J. J. Draaisma, G. Mihov, A. A. Dias, P. J. Schoenmakers, and Sj van der Wal. "Monitoring the in Vitro Enzyme-Mediated Degradation of Degradable Poly(ester amide) for Controlled Drug Delivery by LC-ToF-MS." Biomacromolecules 12, no. 9 (September 12, 2011): 3243–51. http://dx.doi.org/10.1021/bm200709r.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Ahmad, Norlaily, Burcu Colak, Martin John Gibbs, De-Wen Zhang, C. Remzi Becer, Michael Watkinson, Julien E. Gautrot, and Steffi Krause. "Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films." Proceedings 2, no. 13 (November 30, 2018): 961. http://dx.doi.org/10.3390/proceedings2130961.

Full text

Abstract:

Peptide cross-linked poly(ethylene glycol) hydrogel is a promising biomaterial that has been used widely for drug delivery and tissue engineering. However, the use of this material as a biosensor material for the detection of collagenase has not been explored. Collagenase play a key role in rheumatoid arthritis and osteoarthritis. Detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals and their degradation in the presence of collagenase was monitored using a Quartz Crystal Microbalance (QCM). The biosensor was shown to respond to concentrations between 2 nM to 2000 nM with a lower detection limit of 2 nM.

APA, Harvard, Vancouver, ISO, and other styles

47

Clegg, John R., Afshan S. Irani, Eric W. Ander, Catherine M. Ludolph, Abhijeet K. Venkataraman, Justin X. Zhong, and Nicholas A. Peppas. "Synthetic networks with tunable responsiveness, biodegradation, and molecular recognition for precision medicine applications." Science Advances 5, no. 9 (September 2019): eaax7946. http://dx.doi.org/10.1126/sciadv.aax7946.

Full text

Abstract:

Formulations and devices for precision medicine applications must be tunable and multiresponsive to treat heterogeneous patient populations in a calibrated and individual manner. We engineered modular poly(acrylamide-co-methacrylic acid) copolymers, cross-linked into multiresponsive nanogels with either a nondegradable or degradable disulfide cross-linker, that were customized via orthogonal chemistries to target biomarkers of an individual patient’s disease or deliver multiple therapeutic modalities. Upon modification with functional small molecules, peptides, or proteins, these nanomaterials delivered methylene blue with environmental responsiveness, transduced visible light for photothermal therapy, acted as a functional enzyme, or promoted uptake by cells. In addition to quantifying the nanogels’ composition, physicochemical characteristics, and cytotoxicity, we used a QCM-D method for characterizing nanomaterial degradation and a high-throughput assay for cellular uptake. In conclusion, we generated a tunable nanogel composition for precision medicine applications and new quantitative protocols for assessing the bioactivity of similar platforms.

APA, Harvard, Vancouver, ISO, and other styles

48

Han, Wenjun, Jingyan Gu, Yuanyuan Cheng, Huihui Liu, Yuezhong Li, and Fuchuan Li. "Novel Alginate Lyase (Aly5) from a Polysaccharide-Degrading Marine Bacterium, Flammeovirga sp. Strain MY04: Effects of Module Truncation on Biochemical Characteristics, Alginate Degradation Patterns, and Oligosaccharide-Yielding Properties." Applied and Environmental Microbiology 82, no. 1 (October 30, 2015): 364–74. http://dx.doi.org/10.1128/aem.03022-15.

Full text

Abstract:

ABSTRACTAlginate lyases are important tools for oligosaccharide preparation, medical treatment, and energy bioconversion. Numerous alginate lyases have been elucidated. However, relatively little is known about their substrate degradation patterns and product-yielding properties, which is a limit to wider enzymatic applications and further enzyme improvements. Herein, we report the characterization and module truncation of Aly5, the first alginate lyase obtained from the polysaccharide-degrading bacteriumFlammeovirga. Aly5 is a 566-amino-acid protein and belongs to a novel branch of the polysaccharide lyase 7 (PL7) superfamily. The protein rAly5 is an endolytic enzyme of alginate and associated oligosaccharides. It prefers guluronate (G) to mannuronate (M). Its smallest substrate is an unsaturated pentasaccharide, and its minimum product is an unsaturated disaccharide. The final alginate digests contain unsaturated oligosaccharides that generally range from disaccharides to heptasaccharides, with the tetrasaccharide fraction constituting the highest mass concentration. The disaccharide products are identified as ΔG units. While interestingly, the tri- and tetrasaccharide fractions each contain higher proportions of ΔG to ΔM ends, the larger final products contain only ΔM ends, which constitute a novel oligosaccharide-yielding property of guluronate lyases. The deletion of the noncatalytic region of Aly5 does not alter its M/G preference but significantly decreases the enzymatic activity and enzyme stability. Notably, the truncated protein accumulates large final oligosaccharide products but yields fewer small final products than Aly5, which are codetermined by its M/G preference to and size enlargement of degradable oligosaccharides. This study provides novel enzymatic properties and catalytic mechanisms of a guluronate lyase for potential uses and improvements.

APA, Harvard, Vancouver, ISO, and other styles

49

Ahmad, Norlaily, Burcu Colak, De-Wen Zhang, Martin John Gibbs, Michael Watkinson, C. Remzi Becer, Julien E. Gautrot, and Steffi Krause. "Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase." Sensors 19, no. 7 (April 8, 2019): 1677. http://dx.doi.org/10.3390/s19071677.

Full text

Abstract:

Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.

APA, Harvard, Vancouver, ISO, and other styles

50

Amir, Roey, Assaf Harnoy, Nitsan Papo, and Gadi Slor. "Mixing End Groups in Thiol-Ene/Yne Reactions as a Simple Approach toward Multienzyme-Responsive Polymeric Amphiphiles." Synlett 29, no. 19 (November 16, 2018): 2582–87. http://dx.doi.org/10.1055/s-0037-1611340.

Full text

Abstract:

Taking advantage of the high fidelity of thiol-ene and thiol-yne chemistries, we used mixtures of thiols to prepare degradable PEG-dendron amphiphiles functionalized with two different types of enzymatically cleavable end groups. By tuning the feed ratios of the two thiols, we achieved mixtures of hybrids with statistically different ratios of end groups. Studies of the disassembly of statistically mixed hybrids showed that these amphiphiles have higher degrees of response when incubated with each of the activating enzymes, whereas a greater degree of selectivity was observed for a control mixture of two distinct amphiphiles, which required the presence of both types of enzyme to undergo complete disassembly. The potential to introduce different end groups by using a mixture of thiols in an efficient single thiol-ene or thiol-yne step opens the way for simple modification of various ene- or yne-containing polymers and tailoring of their structural and functional properties.

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!