Academic literature on the topic 'Enzyme immunoassay techniques'

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Journal articles on the topic "Enzyme immunoassay techniques"

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Porstmann, T., and S. T. Kiessig. "Enzyme immunoassay techniques an overview." Journal of Immunological Methods 150, no. 1-2 (1992): 5–21. http://dx.doi.org/10.1016/0022-1759(92)90061-w.

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Kaufman, Bennett M., and Marion Clower. "Immunoassay of Pesticides: An Update." Journal of AOAC INTERNATIONAL 78, no. 4 (1995): 1079–90. http://dx.doi.org/10.1093/jaoac/78.4.1079.

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Abstract Measurement of levels of pesticide residues in foods and crops most often requires extensive cleanup and instrumental techniques such as gas chromatography. Immunoassay measurement techniques, on the other hand, may be used directly on the test portion or require only minimal cleanup. Further refinements of the common antibody–enzyme-based solid-phase assays, such as use of coated magnetic particles, antibody-coated crystals, and continuous-flow devices, have extended the measurement range and applicability of these assays. Likewise, new immunoassays for pesticides have been developed
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Dinter, Z. "Enzyme Immunoassay Techniques, ELISA, in animal and plant diseases." Veterinary Microbiology 21, no. 3 (1990): 294–95. http://dx.doi.org/10.1016/0378-1135(90)90043-u.

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Siqueira, Marilda M., Vanja Ferreira, and Jussara P. Nascimento. "RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay." Memórias do Instituto Oswaldo Cruz 81, no. 2 (1986): 225–32. http://dx.doi.org/10.1590/s0074-02761986000200013.

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Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolaion in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory iliness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortu
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Li, Li, Wenbo Lu, Chang Liu, Ying Wang, Jian Dong, and Weiping Qian. "Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres." Nano 12, no. 08 (2017): 1750092. http://dx.doi.org/10.1142/s1793292017500928.

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The sandwich-type immunoassays have been developed by using electrochemical and surface-enhanced Raman scattering (SERS) techniques for the detection of carcinoembryonic antigen (CEA). Nile blue as a kind of Raman dye has been decorated on nanospheres with polydopamine resin (PDR) via [Formula: see text]-stacking interaction. The Nile blue displays the strong SERS signals as well as a characteristic electrochemical reduction peak at [Formula: see text]0.33[Formula: see text]V (versus Ag/AgCl). The implementation of the PDR nanospheres mixing with Au nanoparticles (AuNPs/PDR) exhibits a better
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Usleber, Ewald, Richard Dietrich, Christine Bürk, Elisabeth Schneider, and Erwin Märtlbauer. "Immunoassay Methods for Paralytic Shellfish Poisoning Toxins." Journal of AOAC INTERNATIONAL 84, no. 5 (2001): 1649–56. http://dx.doi.org/10.1093/jaoac/84.5.1649.

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Abstract The current status of immunochemical techniques for analysis of paralytic shellfish poisoning (PSP) toxins is summarized. Important aspects regarding production of the biological reagents necessary for immunochemical methods, the characteristics of polyclonal and monoclonal antibodies against saxitoxin and neosaxitoxin, and the importance of test sensitivity and specificity are discussed. Applications of immunochemical techniques for PSP toxins include microtiter plate enzyme immunoasays and enzyme-linked immunofiltration assays for toxin detection, and immunoaffinity chromatography (
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Créminon, Christophe, and Frédéric Taran. "Enzyme immunoassays as screening tools for catalysts and reaction discovery." Chemical Communications 51, no. 38 (2015): 7996–8009. http://dx.doi.org/10.1039/c5cc00599j.

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This feature article summarizes the development and use of immunoassay techniques (ELISA) as screening tools for fast identification of efficient catalysts in libraries and for the discovery of new chemical reactions.
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Henderson, D. R., S. B. Friedman, J. D. Harris, W. B. Manning, and M. A. Zoccoli. "CEDIA, a new homogeneous immunoassay system." Clinical Chemistry 32, no. 9 (1986): 1637–41. http://dx.doi.org/10.1093/clinchem/32.9.1637.

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Abstract Genetic engineering of beta-galactosidase (EC 3.2.1.23) has led to the development of a new homogeneous assay system, CEDIA. The Z gene of the lac operon of Escherichia coli encodes a large enzymatically inactive polypeptide that spontaneously aggregates and folds to form active beta-galactosidase. Using recombinant DNA techniques, we have been able to engineer beta-galactosidase protein into a large polypeptide (an enzyme acceptor, EA) and a small polypeptide (an enzyme donor, ED). The EAs and EDs are both enzymatically inactive, but spontaneously associate to form enzymatically acti
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Cao, Zhimin (Tim), Thomas A. Swift, Clint A. West, Thomas G. Rosano, and Robert Rej. "Immunoassay of Estradiol: Unanticipated Suppression by Unconjugated Estriol." Clinical Chemistry 50, no. 1 (2004): 160–65. http://dx.doi.org/10.1373/clinchem.2003.023325.

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Abstract Background: Accurate measurement of estradiol is important in clinical settings. The quality of laboratory estimations of estradiol may be assessed through external quality-assurance surveys. Methods: Estradiol was measured by microparticle enzyme immunoassay (MEIA) and other immunoassays. Proficiency testing of medical laboratories was conducted using samples prepared from normal male human serum supplemented with exogenous estradiol and other steroid and nonsteroid hormones, and participant laboratories measured estradiol by a variety of commonly used immunoassay techniques. Results
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Chang, Zhu, Zhi Wu Shang, Zhen Wu Liu, and Tai Yong Wang. "A Small Automated Enzyme Immunoassay Analyzer System Design and Implementation Based on Immunoenzymatic Techniques." Key Engineering Materials 693 (May 2016): 1308–13. http://dx.doi.org/10.4028/www.scientific.net/kem.693.1308.

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A small automated enzyme immunoassay analyzer system based on immunoenzymatic techniques is designed and implemented in this paper. The working instrument process is determined according to immunoenzymatic techniques, and then enzyme analysis system is designed. Institutional innovation is implemented. Several forms institutions of absorbing and discharging and mobile are designed, according working principle instrument and the forms of the analyzer at home and abroad. A new photoelectric detection institution based on micro spectrometer is designed. Using fuzzy adaptive algorithm controls con
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Dissertations / Theses on the topic "Enzyme immunoassay techniques"

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Lövgren, Ulf. "Enzyme immunoassay in combination with liquid chromatography for sensitive and selective determination of drugs in biosamples." Lund : Dept. of Analytical Chemistry, Lund University, 1997. http://books.google.com/books?id=Ju1qAAAAMAAJ.

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Jones, Owen G. "Comparison of analytical techniques for quantification of 8-iso-PGF2[alpha] using HPLC-MS-MS and enzyme immunoassay." Connect to this title online, 2005. http://hdl.handle.net/1811/347.

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Thesis (Honors)--Ohio State University, 2005<br>Title from first page of PDF file. Document formattted into pages: contains, 16 p.; also includes graphics. Includes bibliographical references (p. 15-16) Available online via Ohio State University's Knowledge Bank.
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Nording, Malin. "Rapid sample preparation and bioanalytical techniques for efficient screening of organic pollutants in the environment." Doctoral thesis, Umeå universitet, Kemi, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-842.

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Large numbers of samples often need to be prepared and analysed in surveys of organic pollutants in the environment, but while the methods commonly used in such surveys can provide abundant detail they are generally costly, time-consuming and require large amounts of resources, so there is a need for simpler techniques. The work underlying this thesis assessed the potential utility of more convenient sample preparation and bioanalytical techniques for rapidly screening various environmental matrices that could be useful complements to higher resolution methods. Initially, the utility of a simp
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Chiu, May Leung. "Investigation and characterization of analyte-reporter enzyme conjugates for the enzyme-multiplied immunoassay technique." Diss., Restricted to subscribing institutions, 2009. http://proquest.umi.com/pqdweb?did=1930910331&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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KATO, KANEFUSA, HIDEO YAMADA, and AKIHITO HIRANO. "Quantitation of Red-Cell-Bound IgG in Normal and Pathologic States by an Enzyme Immunoassay (EIA) Technique." Nagoya University School of Medicine, 1985. http://hdl.handle.net/2237/17472.

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Zrein, Maan. "Applications de nouvelles methodes immuno-enzymatiques de diagnostic en virologie humaine et vegetale." Strasbourg 1, 1986. http://www.theses.fr/1986STR13088.

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Dekker, Elise. "Utilisation des anticorps monoclonaux pour l'etude de quelques tobamovirus et geminivirus." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13143.

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Goumas, Dimitrios. "Possibilites de detection d'erwinia chrysanthemi pv. Dianthicola (hellmers) dickey 1979-agent de la bacteriose du dahlia sp. Evaluation des methodes immunoenzymatiques pour le controle sanitaire du materiel de propagation." Paris 6, 1987. http://www.theses.fr/1987PA066405.

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La bacteriose a e. Chrysanthemi pv. Dianthicola (echr) facteur limitant de la production du dahlia est transmise par la multiplication vegetative. Afin de proposer une methode de diagnostic plus precise que la detection visuelle, les methodes immunoenzymatiques ont ete etudiees et adaptees pour la detection d'echr dans les tissus du dahlia. La methode das-elisa (double antibody sandwich) est evaluee par rapport aux methodes de diagnostic de reference (isolement et immunofluorescence). Son utilisation, pour l'analyse sanitaire du materiel de propagation vis-a-vis d'echr seul et associe eventuel
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Books on the topic "Enzyme immunoassay techniques"

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M, Cambra Alvarez, International Office of Epizootics, and Instituto Nacional de Investigaciones Agrarias., eds. Enzyme immunoassay techniques, ELISA, in animal and plant diseases. 2nd ed. Office international des épizooties, 1987.

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Nkohkwo, Asa'ah Tazanu. Development of a membrane-based amperometric detection technique for measuring conjugate levels in enzyme-labelled immunoassays. University of Manchester, 1993.

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Ishikawa, E. Ultrasensitive and Rapid Enzyme Immunoassay (Laboratory Techniques in Biochemistry and Molecular Biology). Elsevier Science, 1999.

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Ishikawa, E. Ultrasensitive and Rapid Enzyme Immunoassay (Laboratory Techniques in Biochemistry and Molecular Biology). Elsevier Science, 1999.

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1944-, Ngo T. T., and Lenhoff Howard M, eds. Enzyme-mediated immunoassay. Plenum Press, 1985.

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Wimpy, Thomas Harold. An enzyme immunoassay for milk progesterone: Development of a flow-through technique. 1988.

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Giovanni, Di Sabato, ed. Immunochemical techniques. Academic Press, 1988.

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Burdon. Practice and Theory of Enzyme Immunoassays (Laboratory Techniques in Biochemistry and Molecular Biology, Vol 15). Elsevier Science Ltd, 1985.

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Burdon. Practice and Theory of Enzyme Immunoassays (Laboratory Techniques in Biochemistry and Molecular Biology, Vol 15). Elsevier Science Ltd, 1985.

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Methods in Enzymology, Volyme 163: Immunochemical Techniques, Part M: Chemotaxis and Inflammation. Academic Press, 1988.

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Book chapters on the topic "Enzyme immunoassay techniques"

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Hsu, Su-Ming. "Immunoperoxidase Techniques Using the Avidin-Biotin System." In Enzyme-Mediated Immunoassay. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5012-5_26.

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Alhabbab, Rowa Yousef. "Enzyme Immunoassay (EIAs) and Enzyme-Linked Immunosorbent Assay (ELISA)." In Techniques in Life Science and Biomedicine for the Non-Expert. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-77694-1_12.

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Maclouf, J., J. Grassi, and P. Pradelles. "Development of Enzyme-Immunoassay Techniques for Measurement of Eicosanoids." In Prostaglandin and Lipid Metabolism in Radiation Injury. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-5457-4_37.

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Deshpande, S. S. "Conjugation Techniques." In Enzyme Immunoassays. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-1169-0_4.

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Monroe, Dan. "Enzyme Channelling Immunoassay (ECIA): A Unique and Rapid Quantitative Technique." In Reviews on Immunoassay Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11009-4_6.

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Yokota, Sadaki. "Immunocytochemical Localization of Peroxisomal Enzymes in Rat Liver and Kidney revealed by Immunoenzyme and Immunogold Techniques." In Reviews on Immunoassay Technology. Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-11009-4_10.

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Nieuwenhuizen, W. "Quantitative enzyme immunoassays for degradation products of fibrinogen (FgDP), fibrin (FbDP) and total of FgDP and FbDP (TDP)." In ECAT Assay Procedures A Manual of Laboratory Techniques. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2992-3_24.

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Maier, R. D. "Praxisorientierte Erfahrungen beim Cannabinoidnachweis im Urin mit der „Enzyme multiplied Immunoassay Technique“ (EMIT-st) und HPLC-Absicherung." In Medizin und Recht. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71456-6_75.

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Spickett, Gavin P. "Autoantibodies." In Oxford Handbook of Clinical Immunology and Allergy. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199603244.003.0018.

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Introduction Techniques: overview Particle agglutination assays Immunoprecipitation assays Indirect immunofluorescence Direct immunofluorescence Radio-immunoassay (RIA) Enzyme-linked (EIA) and fluorescent immunoassays (FIA) Immunoblotting Acetylcholine-receptor antibodies (AChRAb) Actin antibodies Adrenal cortex autoantibodies Amphiphysin antibodies Anti-nuclear antibodies (ANA) and ANCA Aquaporin antibodies Auerbach’s plexus antibodies β‎2-GPI antibodies C1q antibodies...
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"Chapter 14 Quantitative enzyme immunoassay techniques." In Laboratory Techniques in Biochemistry and Molecular Biology. Elsevier, 1985. http://dx.doi.org/10.1016/s0075-7535(08)70144-x.

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Conference papers on the topic "Enzyme immunoassay techniques"

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Caragea, Genica, Margarita Forje, Ruxandra Avram, et al. "An optoelectronic biomedical method for amphetamine detection by enzyme multiplied immunoassay technique." In Advanced Topics in Optoelectronics, Microelectronics and Nanotechnologies 2020, edited by Marian Vladescu, Ionica Cristea, and Razvan D. Tamas. SPIE, 2020. http://dx.doi.org/10.1117/12.2571711.

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Moake, J. L., M. A. Harris, C. E. Whitley, and C. P. Alfrey. "RAPID, SENSITIVE N0N-RADI0ACTIVE QUANTIFICATION AND ANALYSIS OF PLASMA VON WILLEBRAND FACTOR (vWF) MULTIMERS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644085.

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Assessment of plasma vWF abnormalities by clinical coagulation laboratories is difficult because the available test systems for vWF antigen quantification and multimer analysis are expensive, laborious, and require days, radioactive anti-vWF antibodies and autoradiographic methods. We have devised simple, rapid, sensitive alternative techniques for vWF quantification and multimer analysis that can be readily installed in clinical laboratories. Plasma vWF antigen quantification is by a 2 hour enzyme immunoassay that accurately detects levels as low as 0.23% of normal. Plasma vWF to be quantifie
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Lateef, Mehreen. "Determination of Serum C-reactive Protein Levels in Breast Cancer by Enzyme Linked Immunoassay Technique." In IBRAS 2021 INTERNATIONAL CONFERENCE ON BIOLOGICAL RESEARCH AND APPLIED SCIENCE. Juw, 2021. http://dx.doi.org/10.37962/ibras/2021/13.

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Stüber, W., H. Pelzer, and N. Heimburger. "INDUCTION OF ANTITHROMBIN III (AT III) ANTIBODIES BY IMMUNIZATION WITH SYNTHETIC PEPTIDES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644355.

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The primary structure of AT III was examined in respect of potential antigenic sites. The topics were the determination of the hydrophilicity, hydropathy, acrophilicity and the propensities for alpha-helices, B-turns and 13-sheets. The peptides AT III 21-34, 21-42, 129 - 140, 226 - 240 and 343 -363 were synthesized using the solid phase peptide synthesis methode. The subsequent purification of the crude peptides was achieved by h.p.I.e. or by ion exchange chromatography. The peptides were coupled to keyhole limpet hemocyanine (KLH) via thioether bonds. Antisera against KLH-peptides were raised
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Tanaka, I., A. Yoshioka, T. Fujiwara, H. Nakai, and H. Fukui. "THE CHANGES OF FACTOR VIII ANTIGEN DURING THE COAGULATION PROCESS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644038.

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The changes of factor VIII (F. VIII) during blood coagulation process is still controversial. We analyzed the F. VIII antigen (F. VIII:Ag) at various intervals of in vitro blood clotting by immunoassays using polyclonal and different kinds of monoclonal antibodies to F. VIII.We used two immunoassays, an immunoradiometric assay (IRMA) and an enzyme-linked immunosorbent assay (ELISA). The IRMA was performed by the method of Peake et al. using high-titer allo-antibodies to F. VIII. The ELISA was performed by two-site solid phase system consisting of alloantibodies as the first and one of three ki
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