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Relevant bibliographies by topics / Enzyme kinetics / Dissertations / Theses

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Dissertations / Theses on the topic 'Enzyme kinetics'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Zaman, Flora. "Kinetics of enzyme models." Thesis, University of Kent, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263701.

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2

Qian, Yuhui. "Study of Basic Wood Decay Mechanisms and Their Biotechnological Applications." Fogler Library, University of Maine, 2008. http://www.library.umaine.edu/theses/pdf/QianY2008.pdf.

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3

Moore, Robert Goodwin Douglas C. "Towards the understanding of complex biochemical systems the significance of global protein structure and thorough parametric analysis /." Auburn, Ala, 2009. http://hdl.handle.net/10415/1766.

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4

Ekici, Ozlem Dogan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Diss., Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/5333.

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5

Ekici, Özlem Doğan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Available online, Georgia Institute of Technology, 2004:, 2003. http://etd.gatech.edu/theses/available/etd-04062004-164633/unrestricted/ekici%5Fozlem%5Fd%5F200312%5Fphd.pdf.

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6

Astier, Yann. "Enzyme kinetics and electrochemical polymer transistor detection of enzyme reactions." Thesis, University of Southampton, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273800.

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7

Kakkar, Tarundeep Singh. "Theoretical studies on enzyme inhibition kinetics." Diss., The University of Arizona, 1999. http://hdl.handle.net/10150/289017.

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Enzyme inhibition studies are conducted to characterize enzymes and to examine drug-drug interactions. To characterize the inhibitory process (competitive, non-competitive and uncompetitive) and to determine the inhibitory constant (Kᵢ), data analysis techniques (e.g., Dixon, Lineweaver-Burk, etc.) are used to linearize the inherently non-linear rate of substrate metabolism vs. substrate concentration data. These techniques were developed before the general use of computers. However, many investigators still rely on these techniques in spite of the easy availability of non-linear regression fi

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8

Bayram, Mustafa. "Computer algebra approaches to enzyme kinetics." Thesis, University of Bath, 1993. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357810.

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9

Epstein, Todd Matthew. "Structural and kinetic studies of two enzymes catalyzing phospholipase A2 activity." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file 2.39 Mb., 186 p, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3200538.

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10

Tenney, Joel David. "The kinetics of the chlorine dioxide generation reaction." Thesis, Georgia Institute of Technology, 1988. http://hdl.handle.net/1853/10020.

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11

Fisher, Oriana. "Subcloning, enzymatic characterization, and in silico docking of transglutaminase 2." Waltham, Mass. : Brandeis University, 2009. http://dcoll.brandeis.edu/handle/10192/23253.

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12

Lai, Chung-Jeng. "Fumarate Activation and Kinetic Solvent Isotope Effects as Probes of the NAD-Malic Enzyme Reaction." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc278864/.

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The kinetic mechanism of activation of the NAD-malic enzyme by fumarate and the transition state structure for the oxidation malate for the NAD-malic enzyme reaction have been studied. Fumarate exerts its activating effect by decreasing the off-rate for malate from the E:Mg:malate and E:Mg:NAD:malate complexes. The activation by fumarate results in a decrease in K_imalate and an increase in V/K_malate by about 2-fold, while the maximum velocity remains constant. A discrimination exists between active and activator sites for the binding of dicarboxylic acids. Activation by fumarate is proposed

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13

Finnigan, William John Andrew. "The exploitation of thermophiles and their enzymes for the construction of multistep enzyme reactions from characterised enzyme parts." Thesis, University of Exeter, 2016. http://hdl.handle.net/10871/27323.

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Biocatalysis is a field rapidly expanding to meet a demand for green and sustainable chemical processes. As the use of enzymes for synthetic chemistry becomes more common, the construction of multistep enzyme reactions is likely to become more prominent providing excellent cost and productivity benefits. However, the design and optimisation of multistep reactions can be challenging. An enzyme toolbox of well-characterised enzyme parts is critical for the design of novel multistep reactions. Furthermore, while whole-cell biocatalysis offers an excellent platform for multistep reactions, we are

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14

Williams, Simon-Peter. "Studies of enzyme kinetics and aspects of enzyme structure in vivo using NMR and molecular genetics." Thesis, University of Oxford, 1992. http://ora.ox.ac.uk/objects/uuid:d8baa574-a5d4-45a2-95a2-c141fbf8d277.

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A quantitative understanding of metabolic control depends on a knowledge of the enzymes involved. The extrapolation of studies in vitro to the intact cell is controversial because the intracellular environment is relatively poorly characterised, particularly with respect to the interactions between weakly-associated enzymes. There is a clear need to study enzymes directly in the cell, yet there are few suitable techniques. Metabolites have been very successfully studied in cells by the non-invasive technique of nuclear magnetic resonance (NMR). NMR studies of enzymes in the cell have, however,

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15

Fisher, Amanda Kaye. "Raman Chemometrics and Application to Enzyme Kinetics and Urinalysis." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/92590.

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Raman spectroscopy records the inelastic scattering of photons originating from striking a sample with monochromatic light. Inelastic, or Raman, scattered photons shift in wavelength due to excitation of the vibrational modes of molecules struck by the incident light. The Raman scattered photons are representative of all of the covalent bonds contained within a sample. Raman spectra taken of biological systems such as proteins, bacterial colonies, and liquid waste, are difficult to interpret due to the complexity of their covalent bond landscape and mixtures of molecules in highly variable con

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16

Welsche, Mathias Markus [Verfasser], and Roland [Akademischer Betreuer] Zengerle. "Determination of enzyme kinetics in micro concentration gradients = Bestimmung von Enzym-Kinetiken in Mikro-Konzentrationsgradienten." Freiburg : Universität, 2013. http://d-nb.info/1123475792/34.

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17

Cho, Yong Kweon. "Kinetic and Chemical Mechanism of Pyrophosphate-Dependent Phosphofructokinase." Thesis, University of North Texas, 1988. https://digital.library.unt.edu/ark:/67531/metadc332128/.

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Data obtained from isotope exchange at equilibrium, exchange of inorganic phosphate against forward reaction flux, and positional isotope exchange of 18O from the (βγ-bridge position of pyrophosphate to a (β-nonbridge position all indicate that the pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii has a rapid equilibrium random kinetic mechanism. All exchange reactions are strongly inhibited at high concentrations of the fructose 6-phosphate/Pi and MgPPi/Pi substrate-product pairs and weakly inhibited at high concentrations of the MgPPi/fructose 1,6-bisphosphate

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18

Åström, Nina. "NADH/NAD⁺ analogues and cyclodextrins in enzyme mimicking systems an experimental and computational investigation /." Lund : Organic Chemistry 1, Lund University, 1995. http://catalog.hathitrust.org/api/volumes/oclc/39781586.html.

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19

Olsen, Greta A. "Characterization and modification of fluorogenic substrate coated particles for use as enzyme probes." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/27553.

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20

Pirinccioglu, Necmettin. "Modification of reactivity by supramolecular complex formation." Thesis, University of Kent, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309749.

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21

Morillas, Manuel. "Catalytic pathway and conformational stability of penicillin G acylase." Thesis, University of Newcastle Upon Tyne, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360276.

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22

Motiu, Stefan. "A theoretical study for the reactivation of O2 inhibited [Fe-Fe]-hydrogenase." Cleveland, Ohio : Cleveland State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1234364653.

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Thesis (Ph.D.)--Cleveland State University, 2008.<br>Title from PDF t.p. (viewed on Apr. 6, 2009). Abstract. Includes bibliographical references (p. 99-109). Available online via the OhioLINK ETD Center. Also available in print.

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23

Dogaru, Daniela. "Hydrogenase inhibition by O2 density functional theory/molecular mechanics investigation /." Cleveland, Ohio : Cleveland State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1231721611.

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Thesis (Ph.D.)--Cleveland State University, 2008.<br>Abstract. Title from PDF t.p. (viewed on Apr. 13, 2009). Includes bibliographical references (p. 102-109). Available online via the OhioLINK ETD Center. Also available in print.

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24

Barnes, John Ashley. "Theoretical modelling of transition states for chemical processes : application to enzymic and non-enzymic glycosidic hydrolysis." Thesis, University of Bath, 1994. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261066.

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25

Suharto, Adrian Rinaldi Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Structural studies of giardial arginine deiminase." Awarded by:University of New South Wales. Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/26293.

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Recombinant giardial arginine deiminase (rADI) was characterized. The enzyme was found to have a specific activity of 12 U (mg protein)-1under at pH 7.4 and 1 mM arginine. The maximum velocity was 14.75 U (mg protein-1) and the KM was 0.167 mM. The specific activity and maximum velocity values are significantly lower than the values reported previously for giardial rADI, while the KM value is quite similar. The optimum pH for the giardial rADI was 6-9, a broad range compared to other arginine deiminases. Recombinant ADI is very specific in its binding specificity, with canavanine (KI 2.4 mM)

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26

Guo, Qi. "Enzyme dynamics and their role in formate dehydrogenase." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/2216.

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How the fast (femtosecond-picosecond, fs-ps) protein dynamics contribute to enzymatic function has gained popularity in modern enzymology. With multiple experimental and theoretical studies developed, the most challenging part is to assess both the chemical step kinetics and the relevant motions at the transition state (TS) on the fast time scale. Formate dehydrogenase (FDH), which catalyzes a single hydride transfer reaction, is a model system to address this specific issue. I have crystallized and solved the structure of FDH from Candida boidinii (CbFDH) in complex with NAD+ and azide. With

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27

Lee, Charles Kai-Wu. "Eurythermalism of a deep-sea symbiosis system from an enzymological aspect." The University of Waikato, 2007. http://hdl.handle.net/10289/2588.

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The recently proposed and experimentally validated Equilibrium Model provides the most detailed description of temperature's effect on enzyme catalytic activity to date. By introducing an equilibrium between Eact, the active form of enzyme, and Einact, a reversibly inactivated form of enzyme, the Equilibrium Model explains apparent enzyme activity loss at high temperatures that cannot be accounted for by irreversible thermal denaturation. The Equilibrium Model describes enzyme behavior in the presence of substrates and under assay conditions; thus its associated parameters, deltaHeq and Teq,

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28

Matosevic, S. "Design and characterisation of a prototype immobilised enzyme microreactor for the quantification of multi-step enzyme kinetics." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/18918/.

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The large number of novel biocatalyst candidates available due to advances in protein engineering and evolution has driven research on automated microwell techniques for rapid catalyst evaluation and quantification of enzyme kinetics and stability. Interest in the further reduction in volume to the microfluidic scale has complemented these microwell approaches for the development of bioprocess operations due to their potential as inexpensive analytical tools with minute volumes and high throughput as well as for their potential for mass replication. This project involves the design and charact

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29

Mallick, Sushanta. "Kinetic mechanism of NAD-malic enzyme from Ascaris suum in the direction of reductive carboxylation of pyruvate." Thesis, University of North Texas, 1990. https://digital.library.unt.edu/ark:/67531/metadc332658/.

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For this pseudoquadreactant enzymatic reaction (Mn2+ is a psuedoreactant), initial velocity patterns were obtained under conditions in which two substrates were maintained saturating while one reactant was varied at several fixed concentrations of the other.

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30

Jones, J. "Kinetic studies on NADsup(+):ADPribosyltransferase." Thesis, University of Reading, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372662.

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31

Hau, Stephen S. "Frequency-domain enzyme kinetics in the context of artificial calcium oscillations." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/39073.

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32

Avis, Johanna M. "Studies on tRNA charging by tyrosyl-tRNA synthetase." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239594.

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33

Yelloly, Julia M. "Ecological role of surface phosphatase activities of Rivulariaceae." Thesis, Durham University, 1996. http://etheses.dur.ac.uk/5086/.

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The literature suggests that the cyanobacterium Rivularia is found at sites where organic phosphorus (P), at times forms a high proportion of total P in the environment. Its ability to utilise organic P through "surface" phosphatase activity may be important in its success. The aim of this thesis was to investigate this in detail using both field material (from a freshwater stream and from a marine intertidal zone) and axenic isolates of Rivulariaceae. At both sites inorganic P concentrations peaked: in March/May (1992-4) at the freshwater site, and in June (1992-3) at the marine site (althoug

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34

Davies, Elizabeth. "The effects of pulsed magnetic fields on the adenylate cyclase enzyme system in aggregating Dictyostelium discoideum." Thesis, University of Brighton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239721.

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35

Howard, Bruce Riley. "The crystal structure of malate synthase and mechanistic implications /." view abstract or download file of text, 1999. http://wwwlib.umi.com/cr/uoregon/fullcit?p9948022.

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Thesis (Ph. D.)--University of Oregon, 1999.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 67-71). Also available for download via the World Wide Web; free to University of Oregon users. Address: http://wwwlib.umi.com/cr/uoregon/fullcit?p9948022.

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36

Borda, Emily J. "Investigation of ribozyme structure and dynamics through photochemical crosslinking and metal ion cleavage /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/11616.

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37

Sharma, Narayan Prasad. "STRUCTURE/FUNCTION STUDIES ON METALLO-B- LACTAMASE ImiS FROM Aeromonas bv. sobria." Oxford, Ohio : Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1181583976.

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38

Lin, Man Guang 1966. "Continuous flow microwave heating : evaluation of system efficiency and enzyme inactivation kinetics." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82281.

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A continuous flow microwave heating system was set up by using one domestic microwave oven (1000W nominal output at 2450MHz). Water was run through the coil centrally located inside the oven cavity for microwave heating. Microwave absorption efficiency was evaluated by measuring inlet and outlet temperatures of coil as a function of system variables. In order to optimize the coil configuration, the influence of tube diameter (6.4, 7.9 and 9.7mm); initial temperature (10, 20 and 30 ºC); number of turns (3.5, 4.5 and 5.5); coil diameter (61.5, 88, 102, 121 and 153 mm) and pitch (16, 18, 2

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39

Rostami-Hodjegan, Amin. "Kinetics of metabolites : implications in the assessment of bioequivalence and enzyme activity." Thesis, University of Sheffield, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245578.

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40

Mehta, Bhavya Chandrakant. "Optimization of enzyme dissociation process based on reaction diffusion model to predict time of tissue digestion." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1142575553.

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41

Scotney, Pierre David. "The catalytic mechanism of Bacillus stearothermophilus pyruvate kinase." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266959.

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42

Downey, Aaron. "Synthesis and MAO activity of a series of benzimidazolyl and indazolyl prodrugs." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/35612.

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Parkinson's disease (PD) is a chronic, progressive disorder of the central nervous system that affects approximately 1.5 million Americans. One of the principal pathological features of PD is dopamine deficiency in the substantia nigra of the brain. A key enzyme that has been associated with the neurodegeneration seen in PD is monoamine oxidase-B (MAO-B). Several inhibitors of this enzyme have resulted in neuroprotection in the mouse model of PD. One such compound is 7-nitroindazole (1). This thesis describes the synthesis and MAO activity of several indazolyl and benzimidazolyl prodrugs tha

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43

Jennings, Natalie A. "Acetylcholinesterase in the sea urchin Lytechinus variegatus characterization and developmental expression in larvae /." Birmingham, AL : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2008r/jennings.pdf.

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44

Collett, Michael. "An allosteric network within dynamin." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/15871.

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Dynamins are large enzymes that catalyse the hydrolysis of GTP (GTPase activity). They assemble through oligomerisation into helical polymers during endocytosis to facilitate membrane scission and vesicle release in all known eukaryotic cell types. Dynamin oligomerisation is associated with an increase in GTPase activity. This drives constriction of the dynamin polymer to induce membrane fission at the neck of endocytic vesicles, releasing the new vesicles into the cytoplasm. This requires GTP hydrolysis at multiple G domains within the helix to be coordinated. The underlying mechanisms that r

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45

Stockert, Amy L. "Spectroscopic and kinetic studies of bovine xanthine oxidase and Rhodobacter capsulatus xanthine dehydrogenase." Connect to this title online, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1089910515.

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Thesis (Ph. D.)--Ohio State University, 2004.<br>Title from first page of PDF file. Document formatted into pages; contains xv, 172 p.; also includes graphics. Includes bibliographical references (p. 165-172).

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46

Yu, Peng. "Allosteric regulation of glycerol kinase: fluorescence and kinetics studies." Diss., Texas A&M University, 2003. http://hdl.handle.net/1969.1/1537.

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Glycerol kinase (GK) from Escherichia coli is allosterically controlled by fructose 1,6-bisphosphate (FBP) and the glucose-specific phosphocarrier protein IIAGlc of the phosphotransferase system. These controls allow glucose to regulate glycerol utilization. Fluorescence spectroscopic and enzyme kinetic methods are applied to investigate these allosteric controls in this study. The linkage between FBP binding and GK tetramer assembly is solved by observation of homo-fluorescence energy transfer of the fluorophore Oregon Green (OG) attached specifically to an engineered surface cysteine in GK.

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47

Loftus, Katherine Marie. "Studies of the Structure and Function of E.coli Aspartate Transcarbamoylase." Thesis, Boston College, 2006. http://hdl.handle.net/2345/580.

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Thesis advisor: Evan R. Kantrowitz<br>E.coli Aspartate transcarbamoylase (ATCase) is the allosteric enzyme that catalyzes the committed step of the de novo pyrimidine biosynthesis pathway. ATCase facilitates the reaction between L-aspartate and carbamoyl phosphate to form N-carbamoyl-L-aspartate and inorganic phosphate. The holoenzyme is a dodecamer, consisting of two trimers of catalytic chains, and three dimers of regulatory chains. ATCase is regulated homotropically by its substrates, and heterotropically by the nucleotides ATP, CTP, and UTP. These nucleotides bind to the regulatory chains,

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48

Hamberg, Anders. "Serine Hydrolase Selectivity : Kinetics and applications in organic and analytical chemistry." Doctoral thesis, KTH, Biokemi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-12831.

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The substrate selectivities for different serine hydrolases were utilized in various applications, presented in papers I-VI. The articles are discussed in the thesis in view of the kinetics of the enzyme catalysis involved. In paper I the enantioselectivities towards a range of secondary alcohols were reversed for Candida antarctica lipase B by site directed mutagenesis. The thermodynamic components of the enantioselectivity were determined for the mutated variant of the lipase. In papers II-III Candida antarctica lipase B was engineered for selective monoacylation using two different approach

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49

Apperley, Kim Yang-Ping. "Reversible and Photolabile Inhibitors for Human Tissue Transglutaminase." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36593.

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Tissue transglutaminase (TG2) is a calcium-dependent enzyme that natively catalyses the formation of isopeptidic bonds between protein- or peptide-bound glutamine and lysine residues. Physiologically, it is ubiquitously expressed in tissues, with roles in cellular differentiation, extracellular matrix stabilisation, and apoptosis, among others. However, its unregulated activity has been associated with various pathologies including fibrosis, cancer and celiac disease. Since most pathologies are associated with an increased transamidation activity, efforts have been directed towards the develo

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50

Baloyi, Thembekile Feonah. "Effects of exogenous fibrolytic enzymes on in vitro fermentation kinetics of forage and mixed." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/19895.

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Thesis (MScAgric)--Stellenbosch University, 2008.<br>ENGLISH ABSTRACT: Two in vitro experiments were conducted to evaluate the effect of exogenous fibrolytic enzyme application on dry matter (DM) and neutral detergent fibre (NDF) degradation and gas production (GP) of mature forages and forage-concentrate mixtures. The forages used in the first experiment were lucerne hay (LH), oat hay (OH) and wheat straw (WS). The same forages were used in the second experiment, but they were mixed with a concentrate feed to make three mixtures consisting of 80% (HC), 50% (MC) or 20% (LC) concentrate. The ex

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