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Journal articles on the topic 'Enzyme'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Nixon, Andrew E., Marc Ostermeier, and Stephen J. Benkovic. "Hybrid enzymes: manipulating enzyme design." Trends in Biotechnology 16, no. 6 (1998): 258–64. http://dx.doi.org/10.1016/s0167-7799(98)01204-9.

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2

Johnson, Eachan, and Luet-Lok Wong. "Partial fusion of a cytochrome P450 system by carboxy-terminal attachment of putidaredoxin reductase to P450cam (CYP101A1)." Catalysis Science & Technology 2016, no. 6 (2016): 7549–60. https://doi.org/10.1039/C6CY01042C.

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Cytochrome P450 (CYP) enzymes catalyze the insertion of oxygen into carbon–hydrogen bonds and have great potential for enzymatic synthesis. Application development of class I CYPs is hampered by their dependence on two redox partners (a ferredoxin and ferredoxin reductase), slowing catalysis compared to self-sufficient CYPs such as CYP102A1 (P450BM3). Previous attempts to address this have fused all three components in several permutations and geometries, with much reduced activity compared to the native system. We report here the new approach of fusing putidaredoxin reductase (PdR) to the car

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3

Gaziola, S. A., C. M. Teixeira, A. Ando, L. Sodek, and R. A. Azevedo. "Enzyme isolation and regulation with lysine biosynthesis and degradation in developing seeds of rice." International Rice Research Notes 21, no. 1 (1996): 27–28. https://doi.org/10.5281/zenodo.6999483.

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This article 'Enzyme isolation and regulation with lysine biosynthesis and degradation in developing seeds of rice' appeared in the International Rice Research Notes series, created by the International Rice Research Institute (IRRI) to expedite communication among scientists concerned with the development of improved technology for rice and rice-based systems. The series is a mechanism to help scientists keep each other informed of current rice research findings. The concise scientific notes are meant to encourage rice scientists to communicate with one another to obtain details on the resear

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Pasaribu, Tiurma, A. P. Sinurat, T. Purwadaria, and P. P. Ketaren. "Improving the nutritive values of solid heavy phase to substitute corn in laying hens diet." Jurnal Ilmu Ternak dan Veteriner 14, no. 3 (2013): 167–76. https://doi.org/10.14334/jitv.v14i3.338.

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Solid heavy phase (SHP), a by product material of palm oil factory obtained by ceramic filtration from liquid waste could be used as a feedstuff to replace corn in poultry diet. A series of experiment was carried out to improve nutrient value of the SHP by supplementation of enzymes and amino acids in order to increase the proportion of SHP to substitute corn in layer diet. There are three enzymes i.e.: Balitnak production (BS4), a commercial single enzyme (consist of mannanase) and comercial multienzymes were tested. All the enzymes were mixed with fresh SHP in different dose, dried and groun

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5

Achmadi, Evita Riviani. "Enzymes as Potencial Source for Clean Label Bakery Product: Part 1, Mechanism and Application Single Enzym." Journal of Food and Agricultural Product 2, no. 2 (2022): 57. http://dx.doi.org/10.32585/jfap.v2i2.2708.

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Background: Increased public awareness of consuming healthy food has driven bakery industry to applied production methods and components of food products that are tailored to the market needs. Food product can be positioned as natural, organic, or free from additives/ preservatives which often referred to clean label trend. Bakery industry commonly using chemical emulsifier as component which improve characteristic and quality baked goods. Usage of chemical component is not appropriate with perception of clean label, although it is not yet clear what a clean label exactly means. Chemical emuls

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Jovov, B., N. K. Wills, P. J. Donaldson, and S. A. Lewis. "Vectorial secretion of a kallikrein-like enzyme by cultured renal cells. I. General properties." American Journal of Physiology-Cell Physiology 259, no. 6 (1990): C869—C882. http://dx.doi.org/10.1152/ajpcell.1990.259.6.c869.

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Urinary kallikreins are proteolytic enzymes known to be secreted by distal nephron tubules. In this study, we demonstrate (using the chromogenic tripeptide substrate S 2266) that the renal cell line A6 from Xenopus laevis secretes a kallikrein-like enzyme. Secretion is present only when the cells are grown on filters, and enzyme is secreted only into the apical membrane bathing solution. Enzyme secretion consists of two components, one soybean trypsin inhibitor (SBTI) sensitive (SSBTI) and the other insensitive to SBTI (ISBTI). Both enzymes were inhibited by aprotinin, a kallikrein-like enzyme

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7

Mao, Shucan, Jiawen Jiang, Ke Xiong, et al. "Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry." Foods 13, no. 23 (2024): 3846. http://dx.doi.org/10.3390/foods13233846.

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This review summarizes the latest progress in enzyme preparation, including enzyme design and modification technology, exploration of new enzyme sources, and application of enzyme preparation in food processing, detection, and preservation. The directed evolution technology improved the stability and catalytic efficiency of enzymes, while enzyme immobilization technology enhanced reusability and industrial applicability. Extremozymes and biomimetic enzymes exhibit excellent performance under harsh conditions. In food processing, enzyme preparation can improve food quality and flavor. In food d

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8

Achmadi, Evita Riviani. "Enzymes as Potencial Source for Clean Label Bakery Product: Part 2, Mechanism, Application and Optimization Combination Enzymes." Journal of Food and Agricultural Product 2, no. 2 (2022): 82. http://dx.doi.org/10.32585/jfap.v2i2.2709.

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Background: Food enzyme is important ingredient for bakery industry to improve production process, functional properties and characteristic bakery product. Food enzym can be applied as single or combination enzym based on purpose which is need on processing. Based on several literatures, application combination enzymes more effective than single enzym, due to synergism effect between enzym which combine. Combination enzymes can be mixed from same or different class of enzym, it is depended on selected specification and characteristic enzym. Application of combination enzymes commonly act as pr

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9

March, John B., and Jason Clark. "Enzymes by post—restriction enzyme stability." Nature Biotechnology 18, no. 3 (2000): 243. http://dx.doi.org/10.1038/73590.

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10

Städler, Brigitte, and Alexander N. Zelikin. "Enzyme prodrug therapies and therapeutic enzymes." Advanced Drug Delivery Reviews 118 (September 2017): 1. http://dx.doi.org/10.1016/j.addr.2017.10.006.

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11

Woggon, Wolf-Dietrich. "Lessons from Enzymes and Enzyme Models." CHIMIA 53, no. 5 (1999): 234. https://doi.org/10.2533/chimia.1999.234.

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Results from our laboratory are presented demonstrating the significance of synthetic active-site analogs of metalloproteins to accomplish catalytic enzyme-like reactions and to identify key intermediates of the reaction cycles.

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12

Siregar, Benedicta Lamria, Rexi Sebastian Siallagan, Suwarnita Butar Butar, Bambang Mahmudi, and Elisabeth Sri Pujiastuti. "The Nutrient Content of Eco-enzymes from Mixture of Various Fruit Peels." Agro Bali : Agricultural Journal 7, no. 2 (2024): 475–87. http://dx.doi.org/10.37637/ab.v7i2.1646.

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Today, many institutions and individuals are paying attention to the development of technologies used in sustainable agriculture. One of the technologies is eco-enzyme that can be used as organic fertilizer. Several researchers have studied the use of eco-enzymes in agriculture, but studies on the nutrient content of eco-enzymes are still very limited. This research was conducted to investigate the nutrient content of two eco-enzyme preparations. The eco-enzymes were produced through the fermentation process of water, fruit peels, and molasses with a weight ratio of 10 : 3 : 1. Fruit peels use

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13

R, Kumaravelrajan, Swetha M та Suba V. "Characterization of Immobilized β-Amylase Enzyme Isolated from Sweet Potato and prepared by Entrapment Method". International Journal of Pharmaceutical Sciences and Nanotechnology(IJPSN) 15, № 6 (2022): 6196–203. http://dx.doi.org/10.37285/ijpsn.2022.15.6.2.

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Aim: This study attempted to isolate β-amylase from sweet potato and enzyme immobilized by encapsulation method, and characterized with various parameters.  Methods: The enzyme β-amylase was isolated with phosphate-buffered saline and purified by centrifugation with ammonium sulfate. The purified enzyme was immobilized on chitosan (0.25 g) and sodium alginate (0.25 g) polymers by entrapment method in the presence of calcium chloride (0.5 M). The immobilized enzyme was characterized by a starch hydrolysis test, the optimal pH and temperature were studied and the stability of the immobiliz

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14

Aliyev, Tofiq, and Səbrin Abdullayeva. "The Role of Enzymes in Modern Medicine: Advances, Applications, and Future Directions." Luminis Applied Science and Engineering 2, no. 1 (2025): 72–76. https://doi.org/10.69760/lumin.20250001012.

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Enzymes play a critical role in modern medicine, serving as essential biological catalysts in therapeutic and diagnostic applications. This article explores the use of enzymes in enzyme replacement therapy (ERT), pharmaceutical drug development, and clinical diagnostics. The advancements in biotechnology have led to the development of engineered enzymes with improved stability and efficiency, addressing challenges such as enzyme degradation, immunogenicity, and production costs. Recent innovations, including enzyme immobilization, nanotechnology-based delivery systems, and CRISPR-engineered en

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15

Zhang, Song, Changping Wang, Hong Chang, Qiang Zhang, and Yiyun Cheng. "Off-on switching of enzyme activity by near-infrared light-induced photothermal phase transition of nanohybrids." Science Advances 5, no. 8 (2019): eaaw4252. http://dx.doi.org/10.1126/sciadv.aaw4252.

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The off-on manipulation of enzyme activity is a challenging task. We report a new strategy for reversible off-on control of enzyme activity by near-infrared light. Enzymes acting on macromolecular substrates are embedded with an ultrasmall platinum nanoparticle and decorated with thermoresponsive copolymers, which exhibit upper critical solution temperature (UCST) behavior. The polymer-enzyme nanohybrids form microscale aggregates in solution below the UCST to prevent macromolecular substrates from approaching the enzymes and thus inhibit the enzyme activity, and they disassemble above the UCS

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16

Vovk, H., and T. Nosenko. "Influence of enzymatic treatment parameters on the press pumkin oil yeild and its properties." Scientific Works of National University of Food Technologies 29, no. 1 (2023): 108–18. http://dx.doi.org/10.24263/2225-2924-2023-29-1-10.

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The influence of parameters of pumpkin seeds pretreatment with enzyme preparations of proteolytic and cellulolytic activity on the yield of press oil, its composition and quality parameters were studied in this work. The enzyme preparation PENICILOPEPSIN (Enzym Biotech, Ukraine) with proteolytic activity and CELLULAD (Enzym Biotech, Ukraine) with cellulolytic, hemicellulase and xylanase activity were used for pretreatment. Enzymatic treatment of seeds was carried out with a mixture of these preparations at a ratio of 7:3 for 2 h at a pH 5.2 and 48—54°C. The following parameters of enzymatic pr

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17

Fadlilla, Thanya, MTh Sri Budiastuti, and MMA Retno Rosariastuti. "Potential of Fruit and Vegetable Waste as Eco-enzyme Fertilizer for Plants." Jurnal Penelitian Pendidikan IPA 9, no. 4 (2023): 2191–200. http://dx.doi.org/10.29303/jppipa.v9i4.3010.

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This study aims to determine the source of eco-enzyme raw materials and the most appropriate dilution volume to produce quality eco-enzyme fertilizer for plant growth. 1 (sugar): 3 (fruit and vegetable waste): 10 (water). The resulting eco-enzyme liquid is processed into fertilizer through the dilution process. Eco-enzyme fertilizers from each raw material source contain different organic N, P, K, and C nutrients. Besides that, some enzymes are beneficial to plants. This research is experimental in the laboratory. The experimental design was factorial design using a completely randomized desig

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18

Ginting, N., and R. E. Mirwandhono. "Productivity of Turi (Sesbania grandiflora) as a multi purposes plant by eco enzyme application." IOP Conference Series: Earth and Environmental Science 912, no. 1 (2021): 012023. http://dx.doi.org/10.1088/1755-1315/912/1/012023.

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Abstract Turi (Sesbania grandiflora) is a multi-purpose plant, including leaves for animal feed, flowers for human food and wood for wood pellets. Eco enzyme is produced from the fermentation of fruits containing enzymes and organic acids. The application of Eco enzyme, among others as biocatalist to improve plant productivity. This paper aims to study dilution of Eco Enzym which was applied to Turi plants to increase its productivity This study used a completely randomized design, namely: T1: Eco enzyme dilution 1: 100; T2: 1: 200; T3: 1: 300 whereas there were 5 replications. Parameters were

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19

Chang, Antje, Lisa Jeske, Sandra Ulbrich, et al. "BRENDA, the ELIXIR core data resource in 2021: new developments and updates." Nucleic Acids Research 49, no. D1 (2020): D498—D508. http://dx.doi.org/10.1093/nar/gkaa1025.

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Abstract The BRENDA enzyme database (https://www.brenda-enzymes.org), established in 1987, has evolved into the main collection of functional enzyme and metabolism data. In 2018, BRENDA was selected as an ELIXIR Core Data Resource. BRENDA provides reliable data, continuous curation and updates of classified enzymes, and the integration of newly discovered enzymes. The main part contains &gt;5 million data for ∼90 000 enzymes from ∼13 000 organisms, manually extracted from ∼157 000 primary literature references, combined with information of text and data mining, data integration, and predic

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20

Li, Can, Zhishang Shi, Jinxing Cai, et al. "Synthesis of Phenylboronic Acid-Functionalized Magnetic Nanoparticles for Sensitive Soil Enzyme Assays." Molecules 27, no. 20 (2022): 6883. http://dx.doi.org/10.3390/molecules27206883.

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Soil enzymes, such as invertase, urease, acidic phosphatase and catalase, play critical roles in soil biochemical reactions and are involved in soil fertility. However, it remains a great challenge to efficiently concentrate soil enzymes and sensitively assess enzyme activity. In this study, we synthesized phenylboronic acid-functionalized magnetic nanoparticles to rapidly capture soil enzymes for sensitive soil enzyme assays. The iron oxide magnetic nanoparticles (MNPs) were firstly prepared by the co-precipitation method and then functionalized by (3-aminopropyl)triethoxysilane, polyethylene

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21

Sunaga, H., H. Sugimoto, Y. Nagamachi, and S. Yamashita. "Purification and properties of lysophospholipase isoenzymes from pig gastric mucosa." Biochemical Journal 308, no. 2 (1995): 551–57. http://dx.doi.org/10.1042/bj3080551.

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Two lysophospholipases, named gastric lysophospholipases I and II (enzymes I and II), were purified 3730- and 2680-fold from pig gastric mucosa. The preparations showed 22 and 23 kDa single protein bands on SDS/PAGE respectively. Both enzymes lacked transacylase activity and appeared to exist as monomers. Their activities were not affected by Ca2+, Mg2+ or EDTA. Enzyme I was most active at pH 8.5 and hydrolysed a variety of lysophospholipids including acidic lysophospholipids and the acyl analogue of platelet-activating factor, whereas enzyme II was most active at pH 8 and its activity was con

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22

Dzulqaidah, Intan, Regina Brigita Zanuba, Andi Siti Fatimah Alwi, Arista Rizkika Putri Salsabila, Siswandi Mursidi, and Handa Muliasari. "Ekstraksi dan Uji Aktivitas Enzim Bromelin Kasar dari Buah Nanas." Journal of Agritechnology and Food Processing 1, no. 2 (2021): 80. http://dx.doi.org/10.31764/jafp.v1i2.6974.

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Pineapple (Ananas comosus) is a source of protease enzymes. The protease enzyme present in pineapple is the bromelain enzyme. Bromelain enzymes are widely used in various industrial fields. The purpose of this experiment was to isolate the bromelain enzyme from pineapple plants, to test the activity of the enzyme, and to determine the optimum temperature of the enzyme. Isolation of the bromelain enzyme from pineapple was carried out by precipitating the pineapple fruit filtrate using table salt (NaCl). Then the crude extract of the enzyme obtained was tested on pieces of meat with variations i

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23

Jun, Jin-Sung, Ye-Lim You, Ha-Jun Byun, et al. "Enzyme Activity and Lipogenesis Inhibition by Fermented Grain Using Natural Enzymes." Molecules 28, no. 21 (2023): 7285. http://dx.doi.org/10.3390/molecules28217285.

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This study aims to compare the effects of three enzyme-rich foods, including one fermented (grain enzyme) and two non-fermented foods (enzyme foods 1 and 2), by investigating their antioxidant, anti-inflammatory, and anti-adipogenic properties. Grain enzyme exhibited the highest radical scavenging activity and was rich in antioxidant components, including total polyphenol and total flavonoid contents. Grain enzyme and enzyme foods 1 and 2 inhibited nitric oxide production by 27, 34, and 17%, respectively, at a concentration of 200 μg/mL in LPS-stimulated macrophages. Among the tested enzymes,

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Høst, Amalie Vang, Roberto Morellon-Sterling, Diego Carballares, John M. Woodley, and Roberto Fernandez-Lafuente. "Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions." Catalysts 12, no. 12 (2022): 1570. http://dx.doi.org/10.3390/catal12121570.

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Enzymes have several excellent catalytic features, and the last few years have seen a revolution in biocatalysis, which has grown from using one enzyme to using multiple enzymes in cascade reactions, where the product of one enzyme reaction is the substrate for the subsequent one. However, enzyme stability remains an issue despite the many benefits of using enzymes in a catalytic system. When enzymes are exposed to harsh process conditions, deactivation occurs, which changes the activity of the enzyme, leading to an increase in reaction time to achieve a given conversion. Immobilization is a w

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Kopetzki, E., K. Lehnert, and P. Buckel. "Enzymes in diagnostics: achievements and possibilities of recombinant DNA technology." Clinical Chemistry 40, no. 5 (1994): 688–704. http://dx.doi.org/10.1093/clinchem/40.5.688.

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Abstract We discuss, from an industrial point of view, the scope and possibilities of recombinant DNA technology for "diagnostic enzyme" production and application. We describe the construction of enzyme-overproducing strains and show how to simplify downstream processing, increase product quality and process profitability, improve diagnostic enzyme properties, and adjust enzymes to harsh assay conditions. We also consider some safety and environmental aspects of enzyme production. Other aspects of diagnostic enzymes that we cover are the facilitation of enzyme purification by attachment of sh

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Ariga, Kotoko, Seiya Tsujimura, and Tsutomu Mikawa. "Evaluation of Enzyme Cascade Electrode Reactions at Nanoscale Area." ECS Meeting Abstracts MA2024-02, no. 54 (2024): 3675. https://doi.org/10.1149/ma2024-02543675mtgabs.

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Introduction Enzymes are promising catalysts with high selectivity and low environmental load. Especially, redox enzymes are attracting attentions because of their applicability to electrodes for biosensors and biofuel cells. For example, wearable lactate biosensors which can detect lactate in sweat and use the reaction to drive itself have been developed recently[1]. Various biosensors are already available today. However, most of them use single-enzyme electrodes. Compared to single-enzyme electrodes, multi-enzyme electrodes are expected to improve the performance of conventional biosensors

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27

Pancapalaga, Wehandaka, and Endang Sri Hartati. "PELATIHAN PEMBUATAN ECO ENZYME DI PONDOK PESANTREN DAARUL FIKRI MALANG." Jurnal Pengabdian Masyarakat Bumi Raflesia 5, no. 1 (2022): 777–81. http://dx.doi.org/10.36085/jpmbr.v5i1.3190.

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Eco enzim adalah cairan serba guna hasil fermentasi selama 90 hari dari sisa buah dan sayuran yang di campur dengan gula dan air. Adapun manfaatnya untuk kesehatan manusia,Â pertanian dan kesehatan lingkungan.Â Oleh karena ituÂ tujuan pengabdian ini adalahÂ untuk memperkenalkanÂ dan mensosialisasikan eco enzymeÂ kepadaÂ siswa siswi Â pondok pesantren Daarul Fikri Â dalam memanfaatkan limbah disekitar pondok pesantren, selain itu untuk melatih siswa siswi pondok pesantren Â membuat eco enzyme. Metode pengabdian yang digunakan berupa sosialisasi dan pelatihan pembuatan eco enzym. Metode yang dig

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Antoun, G. R., I. Brglez та D. G. Williamson. "A 17 β-hydroxysteroid dehydrogenase of female rabbit liver cytosol. Purification and characterization of multiple forms of the enzyme". Biochemical Journal 225, № 2 (1985): 383–90. http://dx.doi.org/10.1042/bj2250383.

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Multiple forms of the soluble 17 beta-hydroxysteroid dehydrogenase of female rabbit liver were identified. NAD-dependent and NADP-dependent enzyme activities were separated by affinity chromatography on agarose-immobilized Procion Red HE3B, and three forms of the NADP-dependent enzyme activity were purified by chromatofocusing. These three enzyme forms are charge isomers and have no quaternary structure. The enzymes catalysed the C-17 oxidoreduction of oestrogens and androgens; with all enzyme forms the activity towards androgens was higher than that toward oestrogens. The enzymes also exhibit

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Zhu, Zheng, Song Ling, Qi-Heng Yang, and Lin Li. "The Difference in the Carboxy-Terminal Sequence Is Responsible for the Difference in the Activity of Chicken and Rat Liver Fructose-2,6-Bisphosphatase." Biological Chemistry 381, no. 12 (2000): 1195–202. http://dx.doi.org/10.1515/bc.2000.147.

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Abstract The fructose-2,6-bisphosphatase domain of the bifunctional chicken liver enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase shares approximately 95% amino acid sequence homology with that of the rat enzyme. However, these two enzymes are significantly different in their phosphatase activities. In this report, we show that the COOH-terminal 25 amino acids of the two enzymes are responsible for the different enzymatic activities. Although these 25 amino acids are not required for the phosphatase activity, their removal diminishes the differences in the activities between the tw

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Datta, Rahul, Swati Anand, Amitava Moulick, et al. "How enzymes are adsorbed on soil solid phase and factors limiting its activity: A Review." International Agrophysics 31, no. 2 (2017): 287–302. http://dx.doi.org/10.1515/intag-2016-0049.

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Abstract A majority of biochemical reactions are often catalysed by different types of enzymes. Adsorption of the enzyme is an imperative phenomenon, which protects it from physical or chemical degradation resulting in enzyme reserve in soil. This article summarizes some of the key results from previous studies and provides information about how enzymes are adsorbed on the surface of the soil solid phase and how different factors affect enzymatic activity in soil. Many studies have been done separately on the soil enzymatic activity and adsorption of enzymes on solid surfaces. However, only a

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Li, Jie, Xin Jin, Yang Liu, et al. "Robust enzyme–silica composites made from enzyme nanocapsules." Chemical Communications 51, no. 47 (2015): 9628–31. http://dx.doi.org/10.1039/c5cc02053k.

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Novel enzyme composites are synthesized first by in situ polymerization around enzymes and a subsequent sol–gel process. Both the polymer shell and the silica shell with desired functional moieties provide not only great enzyme protection but also a favorable microenvironment, resulting in significantly enhanced activity and stability.

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Galperin, Michael Y., D. Roland Walker, and Eugene V. Koonin. "Analogous Enzymes: Independent Inventions in Enzyme Evolution." Genome Research 8, no. 8 (1998): 779–90. http://dx.doi.org/10.1101/gr.8.8.779.

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Lieberman, Jack. "Enzymes in Sarcoidosis: Angiotensin-Converting-Enzyme (ACE)." Clinics in Laboratory Medicine 9, no. 4 (1989): 745–56. http://dx.doi.org/10.1016/s0272-2712(18)30602-4.

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Maeda, Masako. "New label enzymes for bioluminescent enzyme immunoassay." Journal of Pharmaceutical and Biomedical Analysis 30, no. 6 (2003): 1725–34. http://dx.doi.org/10.1016/s0731-7085(02)00514-9.

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Sree Kumar, K., Yashesh N. Vaishnav, and Joseph F. Weiss. "Radioprotection by antioxidant enzymes and enzyme mimetics." Pharmacology & Therapeutics 39, no. 1-3 (1988): 301–9. http://dx.doi.org/10.1016/0163-7258(88)90076-9.

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Mebs, D., S. Mieseler, U. Rimpau, C. Vossius, B. König, and S. Benesch. "Enzymes and enzyme inhibitors from marine sponges." Toxicon 33, no. 3 (1995): 304. http://dx.doi.org/10.1016/0041-0101(95)99364-9.

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37

Lee, Hyeryeong, Yuna Bang, and In Seop Chang. "Facilitated Enzymatic Chain Reaction-Based Bioelectrocatalysis via Solid Binding Peptide-Guided Bienzyme Co-Immobilization Strategy." ECS Meeting Abstracts MA2023-01, no. 42 (2023): 2357. http://dx.doi.org/10.1149/ma2023-01422357mtgabs.

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The multienzyme complex in biological systems are highly ordered so that intermediate molecules occurred during enzymatic sequential reaction are efficiently delivered to downstream enzymes without diffused to bulk phase. Recently, the cascadic multienzymes have been adopted to enzymatic electrocatalytic platform to be applied for enzyme-based bioelectronics such as enzyme fuel cell, biosensors, and electrosynthetic system. This cascadic enzyme-electrode, in which interfacial electron transfer (ET) occurs concurrently with inter-enzyme chain reaction, has been regarded promising for the advanc

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Mirzaei, Mitra, та Per Berglund. "Engineering of ωTransaminase for Effective Production of Chiral Amines". Journal of Computational and Theoretical Nanoscience 17, № 6 (2020): 2827–32. http://dx.doi.org/10.1166/jctn.2020.8947.

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ωTransaminases are pyridoxal-5-phosphat (PLP) dependent enzymes having the ability to catalyze the transference of an amino group to a keto compound. These enzymes are used for production of chiral amines which are important building blocks in pharmaceutical industry. There is often a need to improve enzyme properties such as enzyme stability, enzyme specificity and to decrease substrate-product inhibition. Here, protein engineering was applied to improve the enzyme activity of the enzyme from Chromobacterium violaceum Rational-design and site-directed mutagenesis were applied on position of (

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39

Wakano, Fahruddin. "POTENSI ECO-ENZYME DALAM MENINGKATKAN PERTUMBUHAN DAN PRODUKSI TANAMAN." Journal Gallus Gallus 2, no. 3 (2024): 38–44. https://doi.org/10.51978/gallusgallus.v2i3.489.

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ABSTRAK Eco-enzyme merupakan produk hasil fermentasi bahan-bahan organik yang berasal dari limbah-limbah pertanian dan rumah tangga. Eco-enzyme memiliki beragam kegunaan mulai dari sebagai pupuk organik cair hingga sebagai pestisida nabati.eco-enzyme juga dapat digunakan dalam beberapa keperluan kebersihan rumah tangga. Dengan segudang manfaat tersebut penelitian mengenai potensi eco-enzyme telah dilakukan oleh beberapa peneliti dalam kurun waktu beberapa tahun ini, mulai dari pemanfaatan berbagai jenis jenis limbah seperti kulit jeruk, kulit kentang, kulit wortel, kulit bawang hingga limbah-l

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Szczepanowski, Roman H., Renata Filipek, and Matthias Bochtler. "Crystal Structure of a Fragment of Mouse Ubiquitin-activating Enzyme." Journal of Biological Chemistry 280, no. 23 (2005): 22006–11. http://dx.doi.org/10.1074/jbc.m502583200.

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Protein ubiquitination requires the sequential activity of three enzymes: a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin-ligase (E3). The ubiquitin-transfer machinery is hierarchically organized; for every ubiquitin-activating enzyme, there are several ubiquitin-conjugating enzymes, and most ubiquitin-conjugating enzymes can in turn interact with multiple ubiquitin ligases. Despite the central role of ubiquitin-activating enzyme in this cascade, a crystal structure of a ubiquitin-activating enzyme is not available. The enzyme is thought to consist of a

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Hickey, A. M., L. Marle, T. McCreedy, P. Watts, G. M. Greenway, and J. A. Littlechild. "Immobilization of thermophilic enzymes in miniaturized flow reactors." Biochemical Society Transactions 35, no. 6 (2007): 1621–23. http://dx.doi.org/10.1042/bst0351621.

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The exploitation of enzymes for biotransformation reactions for the production of new and safer drug intermediates has been the focus of much research. While a number of enzymes are commercially available, their use in an industrial setting is often limited to reactions that are cost-effective and they are rarely investigated further. However, the development of miniaturized flow reactor technology has meant that the cost of such research, once considered cost- and time-inefficient, would be much less prohibitive. The use of miniaturized flow reactors for enzyme screening offers a number of ad

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Ahmad, Raneem, Jordan Shanahan, Sydnie Rizaldo, Daniel S. Kissel, and Kari L. Stone. "Co-immobilization of an Enzyme System on a Metal-Organic Framework to Produce a More Effective Biocatalyst." Catalysts 10, no. 5 (2020): 499. http://dx.doi.org/10.3390/catal10050499.

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In many respects, enzymes offer advantages over traditional chemical processes due to their decreased energy requirements for function and inherent greener processing. However, significant barriers exist for the utilization of enzymes in industrial processes due to their limited stabilities and inability to operate over larger temperature and pH ranges. Immobilization of enzymes onto solid supports has gained attention as an alternative to traditional chemical processes due to enhanced enzymatic performance and stability. This study demonstrates the co-immobilization of glucose oxidase (GOx) a

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Antoun, G. R., та D. G. Williamson. "Age-dependent changes in the multiple forms of the soluble 17 β-hydroxysteroid dehydrogenase of female rabbit liver". Biochemical Journal 225, № 2 (1985): 391–98. http://dx.doi.org/10.1042/bj2250391.

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The soluble NADP-dependent 17 beta-hydroxysteroid dehydrogenase activity of female rabbit liver increases with the age of the animal, the specific activity of the enzyme in the 56-day-old rabbit being 3 times that of the 28-day-old animal. The increase in activity is accompanied by a change in the molecular heterogeneity of the enzyme. Three forms (enzymes I, II and III) were identified in the liver cytosol of the 56-day-old female rabbit, whereas only one major form (enzyme IIIY) was present in the 28-day-old animal. Peptide maps of the four purified enzymes showed that there were minor diffe

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Gakhar, Lokesh, Zulfiqar A. Malik, Christopher C. R. Allen, David A. Lipscomb, Michael J. Larkin, and S. Ramaswamy. "Structure and Increased Thermostability of Rhodococcus sp. Naphthalene 1,2-Dioxygenase." Journal of Bacteriology 187, no. 21 (2005): 7222–31. http://dx.doi.org/10.1128/jb.187.21.7222-7231.2005.

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ABSTRACT Rieske nonheme iron oxygenases form a large class of aromatic ring-hydroxylating dioxygenases found in microorganisms. These enzymes enable microorganisms to tolerate and even exclusively utilize aromatic compounds for growth, making them good candidates for use in synthesis of chiral intermediates and bioremediation. Studies of the chemical stability and thermostability of these enzymes thus become important. We report here the structure of free and substrate (indole)-bound forms of naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038. The structure of the Rhodococcus enzym

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Pratiwi, Nurma, and Sigit Ardiansyah. "UTILIZATION OF AGRICULTURAL WASTE AS A SUBSTRATE FOR PRODUCING CELLULASE ENZYME BY ASPERGILLUS NIGER." Jurnal Pengembangan Agroindustri Terapan 1, no. 1 (2022): 23–30. http://dx.doi.org/10.25181/jupiter.v1i1.2655.

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Fresh Fruit Bunches (FFB) of oil palm, bran, straw, and bagasse are agricultural wastes whose availability is very abundant in Indonesia. The agricultural waste is lignocellulosic waste which still has economic value if further processing is carried out, namely as a substrate in the production of cellulase enzymes. Cellulase enzymes are commonly used in various industries such as food biotechnology, textiles, animal feed, paper, and agriculture to degrade cellulose with its main products, namely glucose, cellobiose, and cellooligosaccharides. In producing cellulase enzymes, it is necessary to

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Lin, Peng, Hui Yang, Eiji Nakata, and Takashi Morii. "Mechanistic Aspects for the Modulation of Enzyme Reactions on the DNA Scaffold." Molecules 27, no. 19 (2022): 6309. http://dx.doi.org/10.3390/molecules27196309.

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Cells have developed intelligent systems to implement the complex and efficient enzyme cascade reactions via the strategies of organelles, bacterial microcompartments and enzyme complexes. The scaffolds such as the membrane or protein in the cell are believed to assist the co-localization of enzymes and enhance the enzymatic reactions. Inspired by nature, enzymes have been located on a wide variety of carriers, among which DNA scaffolds attract great interest for their programmability and addressability. Integrating these properties with the versatile DNA–protein conjugation methods enables th

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Lee, Sun Hyung, Ji Sook Lim, and Han Seung Kim. "Decomposition of Chlorinated Hydrocarbons Using the Biocatalyst Immobilized by Clay Minerals." Advanced Materials Research 356-360 (October 2011): 1089–92. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.1089.

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Biochemical decomposition of catechol and 4-chlorocatechol, degradation intermediate products of 4-chlorophenol, was investigated using enzymes and their immobilized forms by clay minerals. An oxygenase that can initiate oxidative ring-fission of aromatic compounds was obtained via cloning of its gene (cphA-1) encoding hydroxyquinol dioxygenase contained in Arthrobacter chlorophenolicus A6 and overexpression and purification of the enzyme. The enzyme expressed in vitro was then immobilized onto the clay mineral (montmorillonite). Michaelis-Menten kinetic analysis was conducted to compare the e

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Oro, Carolina E. Demaman, Bruna M. Saorin Puton, Luciana D. Venquiaruto, Rogério Marcos Dallago, and Marcus V. Tres. "Immobilization of Enzymes in Polymeric Materials Based on Polyamide: A Review." Processes 13, no. 1 (2025): 200. https://doi.org/10.3390/pr13010200.

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The immobilization of enzymes in polyamide-based polymeric materials through covalent bonding is an established technique to stabilize and reuse biocatalysts in industrial processes. Traditionally, enzymes are immobilized using crosslinking agents that activate functional groups on both the support and the enzyme, creating strong bonds that securely anchor the enzyme to the surface. While effective for maintaining enzyme activity over multiple cycles, this method can reduce catalytic efficiency due to rigid binding and involves complex activation steps. Recently, in situ immobilization approac

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Vanderstocken, Gilles, Nicholas L. Woolf, Giuseppe Trigiante, Jessica Jackson, and Rory McGoldrick. "Harnessing the Potential of Enzymes as Inhaled Therapeutics in Respiratory Tract Diseases: A Review of the Literature." Biomedicines 10, no. 6 (2022): 1440. http://dx.doi.org/10.3390/biomedicines10061440.

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Respiratory tract diseases (RTDs) are a global cause of mortality and affect patient well-being and quality of life. Specifically, there is a high unmet need concerning respiratory tract infections (RTIs) due to limitations of vaccines and increased antibiotic resistance. Enzyme therapeutics, and in particular plant-based enzymes, represent an underutilised resource in drug development warranting further attention. This literature review aims to summarise the current state of enzyme therapeutics in medical applications, with a focus on their potential to improve outcomes in RTDs, including RTI

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O'Keefe, S. J., W. M. Bennet, A. R. Zinsmeister, and M. W. Haymond. "Pancreatic enzyme synthesis and turnover in human subjects." American Journal of Physiology-Gastrointestinal and Liver Physiology 266, no. 5 (1994): G816—G821. http://dx.doi.org/10.1152/ajpgi.1994.266.5.g816.

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Animal studies have shown that pancreatic enzyme secretion is independent of enzyme synthesis. To investigate this relationship in humans, we have coinfused 14C-labeled leucine tracer with cholecystokinin octapeptide in nine healthy adults for 4 h and measured the rate of appearance of secreted and newly labeled enzymes in the duodenum. Enzyme secretion was well maintained throughout, but newly labeled enzymes only appeared in juice between 75 and 101 min (median time, 86 min), indicating that initial secretion was dependent on the release of zymogen stores and that the median production time

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