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Dissertations / Theses on the topic 'Enzymes – Classification'

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Published: 4 June 2021

Last updated: 6 February 2022

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1

Wang, Yang. "Exploring glycoside hydrolase family 5 (GH5) enzymes." Licentiate thesis, KTH, Glykovetenskap, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-121537.

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In 1990, the classification of carbohydrate-active enzymes (CAZymes) was introduced by the scientist Bernard Henrissat. According to sequence similarity, these enzymes were separated into families with conserved structures and reaction mechanisms. One interesting class of CAZymes is the group of glycoside hydrolases (GHs) containing more than 138000 modules divided into 131 families as of February 2013. One of the most versatile and the largest of these GH families, containing enzymes with numerous biomass-deconstructing activities, is glycoside hydrolase family 5 (GH5). However, for large and

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2

Wende, Sandra, Sonja Bonin, Oskar Götze, Heike Betat, and Mario Mörl. "The identity of the discriminator base has an impact on CCA addition." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172120.

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CCA-adding enzymes synthesize and maintain the C-C-A sequence at the tRNA 3'-end, generating the attachment site for amino acids. While tRNAs are the most prominent substrates for this polymerase, CCA additions on non-tRNA transcripts are described as well. To identify general features for substrate requirement, a pool of randomized transcripts was incubated with the human CCA-adding enzyme. Most of the RNAs accepted for CCA addition carry an acceptor stem-like terminal structure, consistent with tRNA as the main substrate group for this enzyme. While these RNAs show no sequence conservation,

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3

Garet, Gaëlle. "Classification et caractérisation de familles enzymatiques à l'aide de méthodes formelles." Thesis, Rennes 1, 2014. http://www.theses.fr/2014REN1S082/document.

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Cette thèse propose une nouvelle approche de découverte de signatures de familles (et superfamilles) d'enzymes. Dans un premier temps, étant donné un échantillon aligné de séquences appartenant à une même famille, cette approche infère des grammaires algébriques caractérisant cette famille. Pour ce faire, de nouveaux principes de généralisation et de nouvelles classes de langages ont été introduites sur la base de la substituabilité locale. Un algorithme a également été développé à cet effet qui produit une grammaire réduite, conservant la structuration des exemples, d'un langage substituable.

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4

Wei, Ren, and Wolfgang Zimmermann. "Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we?" Universität Leipzig, 2017. https://ul.qucosa.de/id/qucosa%3A21103.

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Petroleum-based plastics have replaced many natural materials in their former applications. With their excellent properties, they have found widespread uses in almost every area of human life. However, the high recalcitrance of many synthetic plastics results in their long persistence in the environment, and the growing amount of plastic waste ending up in landfills and in the oceans has become a global concern. In recent years, a number of microbial enzymes capable of modifying or degrading recalcitrant synthetic polymers have been identified. They are emerging as candidates for the developme

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5

Krause, Udo. "Protein phosphorylation in yeast mitochondria: enzymes, substrates and function." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-123158.

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Protein phosphorylation is one of the major post-translational modifications to allow for signal transmission and fine tuning of metabolism on the cellular proteomic level. As such it is “one of the last instances” to modulate the activity of enzymes and hence to impact the cellular life irrespective of the basic conditions provided by the genome – and epigenome– controlled gene expression. The evolutionary increase in cellular complexity is reflected by highly sophisticated regulatory networks in multicellular eukaryotes based on the transfer of phosphate mostly onto the side chains of serine

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6

Zhang, Yafei, Zhenye Tang, Hui Liu, Cem Emre Ferah, and Keyong Tang. "Effects of different salt-enzymes on opening up of collagen fiber bundles for leather making - 230." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34320.

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Content: Traditional leather industry involves mechanical, chemical and biological processes, and a lot of leather chemicals are widely used annually. In the beamhouse, especially in liming and re-liming, enormous pollution is usually released because of the traditional use of Na2S and lime. Many researchers have devoted to clean production for leather making. In this study, salt-enzyme liming process was studied in modern leather process to remove the inter-fibrillary matter. Three such salts as Na2SO4, NaCl, and MgCl2 were used with such enzymes as neutral protease and cellulase. The enzyme

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7

Mössinger, Christine. "Identification, regulation and physiological role of enzymes involved in triacylglycerol and phosphatidylcholine synthesis on lipid droplets." Doctoral thesis, Max Planck Institute of Molecular Cell Biology and Genetics, 2009. https://tud.qucosa.de/id/qucosa%3A25375.

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Metabolic energy is most efficiently stored as triacylglycerol (TAG). This neutral lipid accumulates mainly within adipose tissues, but it can be stored and used in all types of cells. Within cells it is packed in organelles called lipid droplets (LDs). They consist of a core of neutral lipids like TAG and cholesterol esters, which is surrounded by a phospholipid monolayer that mainly consists of phosphatidylcholine (PC). Attached to or inserted into this monolayer are various proteins, mainly LD specific structural proteins or lipid metabolic enzymes. Though excess uptake of nutrition leads t

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8

Galarza, Betina, M. L. Garro, N. Ferreri, L. Elíades, and R. Hours. "A new fungal isolates applicated to bovine skin in beamhouse processes - 307." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34210.

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Content: Beamhouse area is an important step in leather technology, either in the final quality of the leather or in the contribution to the contamination of effluents. In unhairing process, the use of enzymes as sulphide assistants can reduce sulfhidric gas emissions to reach permitted levels for health. The characterization of enzymatic extracts allows controlling the proteolysis mechanism so that its action does not attack the reticular structure. A new fungal enzyme extracts were applied on submerged bovine skin in the soaking and unhairing steps. Fungal strains were isolated from alkaline

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9

Farnet, Anne-Marie. "Variabilité phénotypique et génétique chez Marasmius quercophilus, basidiomycète colonisant une litière de chêne vert." Aix-Marseille 3, 1998. http://www.theses.fr/1998AIX30037.

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Cette etude constitue une premiere approche de la taxonomie de marasmius quercophilus, basidiomycete de la litiere du chene vert (quercus ilex l. ), en utilisant des criteres phenotypiques et genotypiques. Dans l'analyse phenotypique, les 36 souches recoltees sur le site de la gardiole de rians (var, france) ont ete regroupees en 11 phenons apres traitements statistiques (dendrogramme et analyse factorielle de correspondence). La nature du substratum (feuille de chene vert ou blanc, gland, bois) et la saison de recolte n'ont pas influence leur repartition. L'analyse genetique a mis en evidence

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10

Schlüter, Nadine, Carolina Ganß, Sandra Pötschke, Joachim Klimek, and Christian Hannig. "Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial." Karger, 2012. https://tud.qucosa.de/id/qucosa%3A27494.

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Patients with bulimia nervosa are at high risk for dental erosion. However, not all bulimic patients suffer from erosion, irrespective of the severity of their eating disorder. It is often speculated that differences in the saliva are important, however, little is known about salivary parameters in bulimic patients, particularly directly after vomiting. The aim of the clinical trial was to compare different salivary parameters of subjects suffering from bulimia with those of healthy controls. Twenty-eight subjects participated (14 patients with bulimia nervosa, 7 of them with erosion; 14 match

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11

Delhom, Bernard. "Potentialités de la cutinase de Fusarium solani pisi pour la transformation chimio-enzymatique de polyols : un exemple de l'utilisation d'hydrolases dans les bioconversions." Compiègne, 1994. http://www.theses.fr/1994COMPD691.

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L'utilisation d'enzymes dans les réactions de synthèse organique devient de plus en plus importante. Les biocatalyseurs s'avèrent particulièrement intéressants en tant que compléments dans la chimie des composés complexes tels que les polyols, en permettant une régio et une stéréospécificité directe sur des substrats protégés ou non. Nous avons utilisé l'estérase recombinante de Fusarium solani pisi dans cette optique. Dans une première partie, nous avons plus particulièrement étudié la réaction de désacétylation d'oses simples et de diols. Les résultats obtenus montrent que cette enzyme n'a p

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12

Al, Qasem Hala. "Molecular Characterization of Carbapenemases and Quinolone Resistance Determining Region Enzymes-Producing Isolates in an Outbreak at the University Hospital of Leipzig." Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-154710.

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Beta lactam resistance producing isolates of Enterobacteriacea and non-Enterobacteriacea have emerged since more than seventy years ago (Abraham and Chain, 1940). They are known to cause both community and hospital-acquired infections. Resistance against carbapenem is primarily mediated by the production of enzymes that destroy the beta lactam antimicrobials, which are produced by these isolates involving the expression of serine and metalobetalactamase genes KPC, IMP, VIM, NDM-1 and OXA-48. Quinolone resistance is predominanty mediated by mutations in the qnrA, qnrB, qnrS, and aac-6-Ib genes.

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13

Ngendahayo, Liboire Désiré. "Identification spécifique des Schistosomes à oeufs à éperon terminal d'Afrique occidentale et centrale." Montpellier 2, 1989. http://www.theses.fr/1989MON20092.

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Des lots, des isolats ou souches de schistosoma bovis, s. Curassoni, s. Haematobium et s. Intercalatum provenant de quinze pays d'afrique occidentale et centrale et de deux pays europeens (espagne et italie-sardaigne) ont ete determines par l'analyse isoenzymatique et/ou par la morphometrie des ufs. Deux methodes ont ete ensuite utilisees en vue d'une identification specifique des quatre especes de schistosomes: les teguments des vers males observes au microscope electronique a balayage et la chetotaxie des cercaires. La premiere methode apporte quelques caracteres differentiels des quatre esp

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14

Jacob, Mandy. "Milchgerinnungsenzyme verschiedener Herkunft und ihr Einfluss auf Käseausbeute und Käsequalität." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-73883.

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Die Dicklegung der Milch, ausgehend von der hydrolytischen Spaltung des κ-Caseins, stellt den ersten essentiellen Schritt in der Käseherstellung dar. Dabei finden Gerinnungsenzyme verschiedener Herkunft Anwendung, deren neueste Varianten auf Grundlage des aktuellen Forschungsstandes umfassend charakterisiert werden. Verschiedene Kälberlabpräparate, mikrobielle Gerinnungsenzyme aus Rhizomucor miehei und mit Hilfe von gentechnisch modifizierten Mikroorganismen gewonnenes Chymosin (FPC) aus Rind und Kamel werden mittels HPLC und Elektrophorese hinsichtlich ihrer Zusammensetzung analysiert. Die ne

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15

Karich, Alexander, Sebastian B. Kleeberg, René Ullrich, and Martin Hofrichter. "Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)—A Substitute of Terephthalic Acid—By the Joined Action of Three Fungal Enzymes." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-234705.

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Enzymatic oxidation of 5-hydroxymethylfurfural (HMF) and its oxidized derivatives was studied using three fungal enzymes: wild-type aryl alcohol oxidase (AAO) from three fungal species, wild-type peroxygenase from Agrocybe aegerita (AaeUPO), and recombinant galactose oxidase (GAO). The effect of pH on different reaction steps was evaluated and apparent kinetic data (Michaelis-Menten constants, turnover numbers, specific constants) were calculated for different enzyme-substrate ratios and enzyme combinations. Finally, the target product, 2,5-furandicarboxylic acid (FDCA), was prepared in a mult

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16

Deumer, Claudia D. "RNA-binding proteins in yeast mitochondria." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2002. http://nbn-resolving.de/urn:nbn:de:swb:14-1035897639531-83407.

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This work focused on the further characterisation of Idhp and of the Krebs cycle enzymes citrate synthase 1 (Cit1p) and malate dehydrogenase 1 (Mdh1p) both of which have been identified as RNA-binding proteins without known RNA recognition motifs. Besides analysing their effects on mitochondrial translation and their organisation in protein complexes the work focused on the characterisation of the RNA-binding properties of recombinant Cit1p and Mdh1p: · Cit1p and Mdh1p play no essential role in mitochondrial protein synthesis. · Idhp is in a complex of molecular weight larger than the cytochro

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17

Fischer, J. D. "Characterisation, classification and conformational variability of organic enzyme cofactors." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599034.

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In order to better understand the chemistry of life, it is important to understand the properties, evolutionary context and functional roles of organic enzymes cofactors. The aim of this work is to investigate this important group of compounds. Which molecules are organic enzyme cofactors? What is their molecular function and enzymatic mechanism? What are their physiochemical properties? How are they different from other metabolites in the cell? Are there intrinsic groupings among them? Which enzyme reactions use them? How variable is their conformation in crystallographic protein structures a

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18

Zhang, Yan. "Chemoenzymatic Resolution in Dynamic Systems : Screening, Classification and Asymmetric Synthesis." Doctoral thesis, KTH, Organisk kemi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-123089.

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This thesis is divided into four parts, all centered around Constitutional Dynamic Chemistry (CDC) and Dynamic Kinetic Resolution (DKR) using biocatalysts for selective transformations, and their applications in screening of bioactive compounds, organic synthesis, and enzyme classification. In part one, an introduction to CDC and DKR is presented, illustrating the basic concepts, practical considerations and potential applications of such dynamic systems, thus providing the background information for the studies in the following chapters. In part two,

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19

Reitz, Martin Johann. "Classification of metabolic reactions based on physicochemical properties and search for enzyme inhibitors." [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=984307923.

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20

Le, Xuan Thinh, Xuan Hien Dang, and Ngoc Trinh Truong. "Effect of phytase on the water quality of Vietnamese pangasius ponds." Technische Universität Dresden, 2018. https://tud.qucosa.de/id/qucosa%3A33314.

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Striped catfish (Pangasianodon hypophthalmus) is freshwater fish that is raising mainly in the Mekong Delta. The research was implemented at the Model Farm (College of Aquaculture and Fisheries), Can Tho University during 6 months from May to November of 2016 and aimed to evaluate the water quality between two treatments of feed in the pangasius production for proposing a better environmental management method. The treatment 1 (control treatment, CT) has two ponds used feed without adding phytase and treatment 2 (experiment treatment, ET) includes two ponds used feed with adding phytase. The r

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21

Ansorge-Schumacher, Marion, and Christoph Loderer. "Enzyme-catalysed regio- and enantioselective preparative scale synthesis of (S)-2-hydroxy alkanones." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-188786.

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α-Hydroxy alkanones were synthesised with high enantiomeric purity by stereoselective enzyme-catalysed diketone reduction. Both diketone reduction and cofactor regeneration were accomplished with purified carbonyl reductase from Candida parapsilosis (CPCR2). The reaction products were isolated by column chromatography and analysed by chiral GC measurements, 1H-NMR spectroscopy and determination of optical rotations. Preparative-scale biotransformations yielded 350–600 mg of pure aliphatic α-hydroxy ketones including the difficult to obtain (S)-2-hydroxypentane-3-one. For all the products good

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22

Werner, Anett, and Susanne Steudler. "Technische Nutzung von Enzymen aus Pilzkulturen." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-202439.

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23

Heindl, Mario, Jan Tuennemann, Ines Sommerer, Joachim Mössner, and Albrecht Hoffmeister. "Loss of Bace1 in mice does not alter the severity of caerulein induced pancreatitis." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-170593.

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Context: Beta-site alpha-amyloid protein cleaving enzyme1 (BACE1) plays a key role in the pathogenesis of Alzheimer’s disease. Additional to its moderate expression in the brain, high levels of BACE1 mRNA were found in the pancreas. Murine Bace1 has been immunohistochemicaly detected at the apical pole of acinar cells within the exocrine pancreas of mice and Bace1 activity was observed in pancreatic juice. In vitro experiments revealed enteropeptidase as a putative substrate for Bace1 suggesting a role in acute pancreatitis.

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24

Yaman, Ayse Gul. "Prediction Of Enzyme Classes In A Hierarchical Approach By Using Spmap." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/2/12610969/index.pdf.

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Enzymes are proteins that play an important role in biochemical reactions as catalysts. They are classified based on the reaction they catalyzed, in a hierarchical scheme by International Enzyme Commission (EC). This hierarchical scheme is expressed as a four-level tree structure and a unique number is assigned to each enzyme class. There are six major classes at the top level according to the reaction they carried out and sub-classes at the lower levels are further specific reactions of these classes. The aim of this thesis is to build a three-level classification model based on the hierarchi

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25

Martin, Melanie. "Inhibitoren des Angiotensin Converting Enzyme (ACE) in hypoallergenen Säuglingsnahrungen." Doctoral thesis, Technische Universität Dresden, 2008. https://tud.qucosa.de/id/qucosa%3A23695.

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Als Schlüsselenzym im Renin-Angiotensin System übernimmt das Angiotensin Converting Enzyme (ACE) eine wichtige Rolle bei der Blutdruckregulierung. ACE spaltet das biologisch inaktive Angiotensin I zum vasokonstriktorisch wirksamen Angiotensin II, was zu einem Anstieg des Blutdruckes führt. Tier- und Humanstudien zeigten, dass die Aufnahme bekannter, aus dem Proteinabbau stammender ACE-Inhibitoren eine Absenkung des Blutdruckes bewirkte. In der Lebensmittelindustrie finden Hydrolysate von Milchproteinen, im speziellen von Molkenproteinen, Einsatz in hypoallergenen (HA) Säuglingsnahrungen. Obwoh

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26

Cao, Shan, J. Song, Y. Shen, Q. Xin, Y. C. Li, and Y. Li. "Development and Investigation of Low Collagen Degradability Unhairing Enzyme by Gene Modification - 21." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34408.

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Content: Unhairing process brought serious pollution, and enzyme application for replacing polluting chemicals in unhairing process attracted much attention in recent years. However, the unhairing enzymes haven’t been accepted widely in actual production due to low purity, complex composition and poor stability. To solve these problems, unhairing enzyme is suggested to be improved by genetic modification in this research. The High-keratinase-producing gene (KerT), which was extracted from B. amyloliquefaciens TCCC11319, was introduced into the B.subtilis WB600 by heterologous expression. Becau

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27

Roth, Christian, Nicole Weizenmann, Nicola Bexten, et al. "Amylose recognition and ring-size determination of amylomaltase." Universität Leipzig, 2017. https://ul.qucosa.de/id/qucosa%3A21099.

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Starch is a major carbon and energy source throughout all kingdoms of life. It consists of two carbohydrate polymers, branched amylopectin and linear amylose, which are sparingly soluble in water. Hence, the enzymatic breakdown by glycoside hydrolases (GHs) is of great biological and societal importance. Amylomaltases (AMs) are GHs specialized in the hydrolysis of a-1,4–linked sugar chains such as amylose. They are able to catalyze an intramolecular transglycosylation of a bound sugar chain yielding polymeric sugar rings, the cycloamyloses (CAs), consisting of 20 to 100 glucose units. Despite

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28

Shen, Yiming, Jinzhi Song, Yanchun Yanchun, and Shan Cao. "Study of the application of wb600-kert in unhairing process - 74." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34270.

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Content: Unhairing process is usually considered as the most polluted process in leather production. The conventional method of unhairing which using lime and sodium sulfide produces a large amount of sludge and waste water. In order to reduce pollution, we developed a novel unhairing enzyme and named as WB600-KerT, which possesses low collagen-degrading ability and high keratin-degrading ability in previous study. The objective of this study is to study the properties and effect of WB600-Kert to replace traditional chemicals in unhairing process. It found that the protease of WB600-Kert exhib

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29

Seele, Jana, Andreas Beineke, Lena-Maria Hillermann, et al. "The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is involved in complement evasion." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-169801.

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Streptococcus (S.) suis is one of the most important pathogens in pigs causing meningitis, arthritis, endocarditis and serositis. Furthermore, it is also an emerging zoonotic agent. In our previous work we identified a highly specific IgM protease in S. suis, designated IdeSsuis. The objective of this study was to characterize the function of IdeSsuis in the host-pathogen interaction. Edman-sequencing revealed that IdeSsuis cleaves the heavy chain of the IgM molecule between constant domain 2 and 3. As the C1q binding motif is located in the C3 domain, we hypothesized that IdeSsuis is involved

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30

Gründel, Anne. "Funktion glykolytischer Enzyme von Mycoplasma pneumoniae in der Wirt-Erreger-Interaktion." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-213500.

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Mycoplasma pneumoniae ist ein parasitär lebendes Bakterium, das eine atypische Pneumonie beim Menschen verursacht. Aufgrund seiner geringen Genomgröße besitzt dieser Organismus einen eingeschränkten Metabolismus sowie eine limitierte Zahl an Pathogenitätsfaktoren. Dennoch ist dieser Mikroorganismus perfekt an seinen Wirt angepasst und es war zu vermuten, dass neben dem komplexen Adhäsionsapparat von M. pneumoniae auch glykolytische Enzyme eine Rolle bei der Interaktion mit humanen Zellen spielen. Diese Enzyme sind maßgeblich bei intrazellulär ablaufenden Stoffwechselprozessen beteiligt. Es wur

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Li, Hao, Deyi Zhu, Yanchun Li, Shan Cao, Changhua Jiang, and Tianping Yu. "Analysis of the Functional Components of Acid Protease and Investigation of Bating Mechanism of Wet-blue - 36." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34266.

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Content: In this study, different acid proteases, which were produced from Aspergillus and Bacillus, were applied for wet-blue bating and their properties and bating effects were observed. The results showed that the acid protease produced Aspergillus had better bating effect and higher chromium tolerance than that of produced by Bacillus. Furthermore, how the acid protease influenced wet-blue microstructure was analyzed by SEM and Micro-CT. The enzymatic properties of acid protease was studied firstly.Zeta potential analysis showed that the isoelectric point (pI) of the protease was consisten

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Gräfe, David, Jens Gaitzsch, Dietmar Appelhans, and Brigitte Voit. "Cross-linked polymersomes as nanoreactors for controlled and stabilized single and cascade enzymatic reactions." Royal Society of Chemistry, 2014. https://tud.qucosa.de/id/qucosa%3A36304.

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Polymeric vesicles or polymersomes are one of the supramolecular entities at the leading edge of synthetic biology. These small compartments have shown to be feasible candidates as nanoreactors, especially for enzymatic reactions. Once cross-linked and equipped with a pH sensitive material, the reaction can be switched off (pH 8) and on (pH 6) in accordance with the increased permeability of the polymersome membranes under acidic conditions. Thus cross-linked and pH sensitive polymersomes provide a basis for pH controlled enzymatic reactions where no integrated transmembrane protein is needed

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33

Sattler, Tatjana, Eveline Wodak, Sandra Revilla-Fernández, and Friedrich Schmoll. "Comparison of different commercial ELISAs for detection of antibodies against porcine respiratory and reproductive syndrome virus in serum." BioMed Central, 2014. https://ul.qucosa.de/id/qucosa%3A13068.

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Background: In recent years, several new ELISAs for the detection of antibodies against the porcine reproductive and respiratory disease virus (PRRSV) in pig serum have been developed. To interpret the results, specificity and sensitivity data as well as agreement to a reference ELISA must be available. In this study, three commercial ELISAs (INgezim PRRS 2.0 - ELISA II, Priocheck® PRRSV Ab porcine – ELISA III and CIVTEST suis PRRS E/S PLUS - ELISA IV, detecting PRRSV type 1 antibodies) were compared to a standard ELISA (IDEXX PRRS X3 Ab Test - ELISA I). The serum of three pigs vaccinated with

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34

Vu, Van Hanh, and Kim Keun. "Hyper-production of raw-starch-digesting enzyme by mutant fungal strain and optimisation of solid by-products." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-99492.

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Selected fungal strain for production of raw-starch-digesting enzyme by solid state fermentation was improved by sequential exposures to γ-irradiation of Co60, ultraviolet and treatments with Nmethyl-N'-nitrosoguanidine. Mutant Aspergillus sp. CXN2-3A was chosen and its production of raw-starch-digesting enzyme (RSDE) was improved 2 folds higher than that of wild type. Optimal condition for the production of the enzyme using wheat bran as the substrate was accomplished for the CXN2-3A. With the optimal fermentation condition and the solid medium supplemented with urea and NH4NO3, CoSO4, Tween

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Franz, Paul, Heike Betat, and Mario Mörl. "Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-204790.

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Background: To allow an immediate treatment of an infection with suitable antibiotics and bactericides or fungicides, there is an urgent need for fast and precise identification of the causative human pathogens. Methods based on DNA sequence comparison like 16S rRNA analysis have become standard tools for pathogen verification. However, the distinction of closely related organisms remains a challenging task. To overcome such limitations, we identified a new genomic target sequence located in the single copy gene for tRNA nucleotidyltransferase fulfilling the requirements for a ubiquitous, yet

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Wolf, Antje. "Einsatz kalorimetrischer Methoden auf Basis integrierter Schaltkreise (IC-Kalorimeter) zur Untersuchung enzymatischer Reaktionen." Doctoral thesis, [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=971623708.

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Dubey, Nidhi Chandrama. "Smart hydrogels based platforms for investigation of biochemical reactions." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-184082.

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Polyketides are natural products with complex chemical structures and immense pharmaceutical potential that are synthesized via secondary metabolic pathways. The in-vitro synthesis of these molecules requires high supply of building blocks such as acetyl Co-enzyme A, and cofactors (adenosine triphosphate (ATP). These precursor and cofactor are synthesized from respective soluble enzymes. Owing to the expensive nature of the enzymes, it is important to immobilize enzymes to improve the process economics by enabling multiple uses of catalyst and improving overall productivity and robustness. The

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Strahler, Jana. "Salivary alpha-amylase: More than an enzyme Investigating confounders of stress-induced and basal amylase activity." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-60778.

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Summary: Salivary alpha-amylase: More than an enzyme - Investigating confounders of stress-induced and basal amylase activity (Dipl.-Psych. Jana Strahler) The hypothalamus-pituitary-adrenal (HPA) axis and the autonomic nervous system (ANS) are two of the major systems playing a role in the adaptation of organisms to developmental changes that threaten homeostasis. The HPA system involves the secretion of glucocorticoids, including cortisol, into the circulatory system. Numerous studies have been published that introduced salivary cortisol to assess HPA axis activity and therefore strengthens i

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Schob, Stefan, Donald Lobsien, Benjamin Friedrich, et al. "The cerebral surfactant system and its alteration in hydrocephalic conditions." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-213883.

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Introduction: Pulmonary Surfactant reduces surface tension in the terminal airways thus facilitating breathing and contributes to host's innate immunity. Surfactant Proteins (SP) A, B, C and D were recently identified as inherent proteins of the CNS. Aim of the study was to investigate cerebrospinal fluid (CSF) SP levels in hydrocephalus patients compared to normal subjects. Patients and methods: CSF SP A-D levels were quantified using commercially available ELISA kits in 126 patients (0±84 years, mean 39 years). 60 patients without CNS pathologies served as a control group. Hydrocephalus pati

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Liu, Xiaoling. "Polymeric Multicompartmentalized Systems Mimicking Artificial Cells for Controllable Multiple Enzymatic Cascade Reactions." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-230515.

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Engineering artificial cells is currently an emerging area of research that involves constructing mimics of biological cells. These biomimetic cellular systems hold tremendous promise for the different biomedical applications (diagnostics, therapy, tissue engineering, gene transfection, bioactive coatings) as well as aspects of synthetic biology. A key architectural principle of the cell is a multicompartmentalized assembly, which is one of the features of biological cells that enable the performance of multiple complex biochemical reactions within confined environments. For this purpose, this

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Sonnendecker, Christian, Ren Wei, Elisabeth Kurze, Jinpeng Wang, Thorsten Oeser, and Wolfgang Zimmermann. "Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli." Unievrsität Leipzig, 2017. https://ul.qucosa.de/id/qucosa%3A21101.

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Background: Cyclodextrin glucanotransferases (CGTases) catalyze the synthesis of cyclodextrins, cyclic oligosaccharides composed of glucose monomers that find applications in the pharmaceutical, food, and cosmetic industries. An economic application of these industrially important enzymes requires their efficient production and recovery. In this study, the effect of Sec-type signal peptides on the recombinant expression of a CGTase derived from Bacillus sp. G825-6 was investigated in Escherichia coli BL21(DE3) using a codon-adapted gene. In addition, a novel purification method for the CGTase

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Sattler, Tatjana, Jutta Pikalo, Eveline Wodak, and Friedrich Schmoll. "Ability of ELISAs to detect antibodies against porcine respiratory and reproductive syndrome virus in serum of pigs after inactivated vaccination and subsequent challenge." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-216172.

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Background: In this study, six enzyme-linked immunosorbent assays (ELISA), intended for routine porcine reproductive and respiratory syndrome virus (PRRSV) herd monitoring, are tested for their ability to detect PRRSV specific antibodies in the serum of pigs after vaccination with an inactivated PRRSV type 1 vaccine and subsequent infection with a highly pathogenic (HP) PRRSV field strain. For this reason, ten piglets (group V) from a PRRSV negative herd were vaccinated twice at the age of 2 and 4 weeks with an inactivated PRRSV vaccine. Ten additional piglets (group N) from the sameherd remai

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Rütten, Simon, Gerald F. Schusser, Getu Abraham та Wieland Schrödl. "Release kinetics of tumor necrosis factor-α and interleukin-1 receptor antagonist in the equine whole blood". Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205268.

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Background: Horses are much predisposed and susceptible to excessive and acute inflammatory responses that cause the recruitment and stimulation of polymorphnuclear granulocytes (PMN) together with peripheral blood mononuclear cells (PBMC) and the release of cytokines. The aim of the study is to develop easy, quick, cheap and reproducible methods for measuring tumor necrosis factor alpha (TNF-α) and interleukin-1 receptor antagonist (IL-1Ra) in the equine whole blood cultures ex-vivo time- and concentration dependently. Results: Horse whole blood diluted to 10, 20 and 50 % was stimulated with

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Zehner, Susanne. "Molekulargenetische und biochemische Untersuchungen zur Tryptophan-5-Halogenase aus der Biosynthese von Pyrroindomycin B in Streptomyces rugosporus." Doctoral thesis, Technische Universität Dresden, 2003. https://tud.qucosa.de/id/qucosa%3A24338.

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Regioselektive Halogenasen sind Enzyme, die für die Biosynthese verschiedener halogenierter Metaboliten verantwortlich sind. Der Stamm Streptomyces rugosporus produziert die bioaktive halogenierte Verbindung Pyrroindomycin B. In dieser Arbeit wurde ein Gen einer Tryptophan-5-Halogenase aus dem Pyrroindomycinproduzenten Streptomyces rugosporus isoliert. Durch gezielte Mutation des Gens konnte die Beteiligung der Halogenase an der Biosynthese von Pyrroindomycin B nachgewiesen werden. Das Tryptophan-5-Halogenase-Gen wurde heterolog exprimiert. In einem spezifischen Enzymtest konnte die Aktivität

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Tischler, Dirk, Michel Oelschlägel, Juliane Zimmerling, and Michael Schlömann. "Neue Wege in der Weißen Biotechnologie." TU Bergakademie Freiberg, 2015. https://tubaf.qucosa.de/id/qucosa%3A23081.

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Mikroorganismen sind in der Lage, zahlreiche Xenobiotika abzubauen. Dazu nutzen sie unter aeroben Bedingungen oft einleitend Oxygenasen. Durch diese kann molekularer Luftsauerstoff aktiviert und auf organische Moleküle übertragen werden. Danach können die Verbindungen in den Metabolismus der Mikroorganismen eingeschleust und teils oder vollständig abgebaut werden. Am Beispiel des Styrols zeigen wir hier eine solche Abbauroute und wie wir diese biotechnologisch nutzen können, um interessante Verbindungen zu synthetisieren. Zielmoleküle der gesamten Enzymkaskade sind dabei diverse Phenylessigsäu

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Raak, Norbert, Raffaele Andrea Abbate, Albena Lederer, Harald Rohm, and Doris Jaros. "Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-234862.

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Casein is the major protein fraction in milk, and its cross-linking has been a topic of scientific interest for many years. Enzymatic cross-linking has huge potential to modify relevant techno-functional properties of casein, whereas non-enzymatic cross-linking occurs naturally during the storage and processing of milk and dairy products. Two size separation techniques were applied for characterisation of these reactions: gel electrophoresis and size exclusion chromatography. This review summarises their separation principles and discusses the outcome of studies on cross-linked casein from the

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von, Buttlar Heiner, Sabine Siegemund, Matthias Büttner, and Gottfried Alber. "Identification of toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells." Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-151442.

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Parapoxvirus ovis (PPVO) is known for its immunostimulatory capacities and has been successfully used to generate vector vaccines effective especially in non-permissive host species. Murine conventional and plasmacytoid dendritic cells (cDC and pDC) are able to recognize PPVO. The PPVO-sensing receptor on pDC is hitherto unknown. In this study we aimed to define the pattern recognition receptor responsible for the activation of murine pDC by inactivated and replication-competent PPVO. We show that PPVO-induced expression of type I and type III interferons, pro-inflammatory cytokines, and costi

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Erfkamp, Jan. "Entwicklung von piezoresistiven Chemo- und Biosensoren auf der Basis von stimuliresponsiven Hydrogelen." TUDpress, 2019. https://tud.qucosa.de/id/qucosa%3A72438.

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Ohne zuverlässige Chemo- und Biosensoren wären beispielsweise die Überwachung von Prozessparametern in der chemischen und biotechnologischen Industrie, die Detektion von geringsten Analytkonzentrationen in der biomedizinischen Analytik oder die Spurenanalyse von Schadstoffen undenkbar. Neue Sensormaterialien wie stimuliresponsive Hydrogele spielen bei der Entwicklung neuer chemischer und biochemischer Sensoren eine immer größere Rolle. Hydrogele sind „intelligente“ hydrophile Polymernetzwerke, die in Abhängigkeit von spezifischen Stimuli quellen und entquellen können. In Kombination mit piezor

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Linning, Philipp, Ute Haussmann, Isaak Beyer, et al. "Optimisation of BACE1 inhibition of tripartite structures by modification of membrane anchors, spacers and pharmacophores – development of potential agents for the treatment of Alzheimer's disease." Royal Society of Chemistry, 2012. https://tud.qucosa.de/id/qucosa%3A27800.

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Systematic variation of membrane anchor, spacer and pharmacophore building blocks leads to an optimisation of the inhibitory effect of tripartite structures towards BACE1-induced cleavage of the amyloid precursor protein (APP).<br>Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.

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Tasso, Mariana Patricia. "Bioactive coatings to control marine biofouling." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-25187.

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The colonization of immersed surfaces by a myriad of marine organisms is a complex, multi-stage, species-specific process giving rise to economic and environmental costs. This unwanted accumulation of organisms in the marine environment, called biofouling, has been attacked from different fronts, going from the ‘problem-elimination-as-problem-solving’ strategy (essentially through the use of biocides) to more elaborated and environmentally-friendly options based on the principle of ‘non-stick’ or ‘easy foul-release’ surfaces, which do not jeopardize marine life viability. Several marine organi

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