Relevant bibliographies by topics / Eosin-nigrosin staining

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Academic literature on the topic 'Eosin-nigrosin staining'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "Eosin-nigrosin staining"

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Kondracki, Stanisław, Anna Wysokińska, Magdalena Kania, and Krzysztof Górski. "Application of two staining methods for sperm morphometric evaluation in domestic pigs." Journal of Veterinary Research 61, no. 3 (2017): 345–49. http://dx.doi.org/10.1515/jvetres-2017-0045.

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Abstract Introduction: The effect of two smear staining methods on the dimensions and shape of sperm cells in the semen of domestic pigs was evaluated. Material and Methods: The studies were carried out on 30 ejaculates collected from 15 boars, which included five Duroc boars, five Pietrain boars, and five hybrid Duroc × Pietrain boars. Each ejaculate was next sampled to make two microscopic slides, of which one was stained with eosin-nigrosin and the other with eosin-gentian dye. In total, 600 measurements of sperm cells were made. Each sperm was measured for the following morphometric parameters: head length, head width, head area, head perimeter, tail length, and the total sperm length. Results: Sperms measured on slides stained with eosin-nigrosin showed lower dimensions as compared with those stained with the eosin-gentian dye method. Sperm stained with eosin-nigrosin had shorter and narrower heads than sperm stained with eosin-gentian dye. The method of staining, therefore, affected not only the dimensions of the sperm, but also the proportions of the dimensions defining the shape of the sperm. Conclusions: The size and shape parameters in porcine sperm may take on different values depending on the method of semen staining. Sperm cells stained with eosin-nigrosin are smaller than the sperm stained with eosin-gentian dye. The sensitivity of the sperm to the type of dye used for the fixation may be associated with genetic factors.
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Gacem, Sabrina, Jaime Catalán, Iván Yánez-Ortiz, Carles Soler, and Jordi Miró. "New Sperm Morphology Analysis in Equids: Trumorph® Vs Eosin-Nigrosin Stain." Veterinary Sciences 8, no. 5 (2021): 79. http://dx.doi.org/10.3390/vetsci8050079.

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The evaluation of the male fertility potential is based on the analysis of the basic spermatic characteristics of concentration, motility and morphology. Thus, the study of sperm morphology is a fundamental element in the seminal analysis, but its real meaning has been biased by the techniques used for its evaluation. These techniques involve dehydration phases and subsequent staining, which involves the production of artifacts. The aim of the study is to compare two methods for equid semen morphology evaluation, Trumorph® using living sperm vs. eosin-nigrosine stain. A total of 49 ejaculates from stallions and donkeys were used. After semen collection and dilution, an aliquot was placed on the slide and introduced in the Trumorph® device. Then observation was made with a 40x objective and negative phase-contrast microscope. Another aliquot was stained using eosin-nigrosine stain and viewed using 100× magnification. Well-formed sperm were observed, and different abnormalities were identified using Trumorph®. The use of eosin-nigrosin staining method and Trumorph® led to the same results and both techniques can be used for stallion and donkey sperm morphological analysis. However, considering the fact that Trumorph® uses living sperm helps prevent sperm cell alteration during sample preparation. Therefore, Trumorph® can be a good alternative to the conventional staining method, which provides a quick test on live sperm.
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Root Kustritz, MV, PN Olson, SD Johnston, and TK Root. "The effects of stains and investigators on assessment of morphology of canine spermatozoa." Journal of the American Animal Hospital Association 34, no. 4 (1998): 348–52. http://dx.doi.org/10.5326/15473317-34-4-348.

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Percentage and types of morphological abnormalities found in canine spermatozoa were evaluated by three investigators using three stains (Giemsa-Wright stain [Diff-Quik], eosin Y/nigrosin [Hancock], and eosin B/nigrosin [Society for Theriogenology morphology stain] with conventional light microscopy, compared to phase contrast microscopy on unstained samples. The percentage of spermatozoa with abnormal heads, midpieces, and tails varied by technique and by investigator. Average percentages of morphologically normal spermatozoa were significantly higher in samples stained with Diff-Quik and samples examined by phase contrast microscopy than in samples stained with Hancock or Society for Theriogenology morphology stains. No effect of investigator on the percentage of morphologically normal spermatozoa was assessed. Results suggest that staining or preparation technique may alter the morphology of canine spermatozoa artifactually.
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Łącka, Katarzyna, Stanisław Kondracki, Maria Iwanina, and Anna Wysokińska. "Assessment of stallion semen morphology using two different staining methods, microscopic techniques, and sample sizes." Journal of Veterinary Research 60, no. 1 (2016): 99–104. http://dx.doi.org/10.1515/jvetres-2016-0014.

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Abstract Introduction: The aim of this study was to propose the optimal methodology for stallion semen morphology analysis while taking into consideration the staining method, the microscopic techniques, and the workload generated by a number of samples. Material and Methods: Ejaculates from eight pure-bred Arabian horses were tested microscopically for the incidence of morphological defects in the spermatozoa. Two different staining methods (eosin-nigrosin and eosin-gentian dye), two different techniques of microscopic analysis (1000× and 400× magnifications), and two sample sizes (200 and 500 spermatozoa) were used. Results: Well-formed spermatozoa and those with major and minor defects according to Blom’s classification were identified. The applied staining methods gave similar results and could be used in stallion sperm morphology analysis. However, the eosin-nigrosin method was more recommendable, because it allowed to limit the number of visible artefacts without hindering the identification of protoplasm drops and enables the differentiation of living and dead spermatozoa. Conclusion: The applied microscopic techniques proved to be equally efficacious. Therefore, it is practically possible to opt for the simpler and faster 400x technique of analysing sperm morphology to examine stallion semen. We also found that the number of spermatozoa clearly affects the results of sperm morphology evaluation. Reducing the number of spermatozoa from 500 to 200 causes a decrease in the percentage of spermatozoa identified as normal and an increase in the percentage of spermatozoa determined as morphologically defective.
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Sousa, Patrícia C., Erika A. A. Santos, Ana L. P. Souza, et al. "Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)." Pesquisa Veterinária Brasileira 33, no. 7 (2013): 924–30. http://dx.doi.org/10.1590/s0100-736x2013000700014.

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The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.
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Mortimer, David. "A technical note on the assessment of human sperm vitality using eosin–nigrosin staining." Reproductive BioMedicine Online 40, no. 6 (2020): 851–55. http://dx.doi.org/10.1016/j.rbmo.2020.03.002.

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Bjorndahl, L. "Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment." Human Reproduction 18, no. 4 (2003): 813–16. http://dx.doi.org/10.1093/humrep/deg199.

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Přinosilová, Petra, Věra Kopecká, Jaroslava Hlavicová, and Monika Kunetková. "Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation." Acta Veterinaria Brno 83, no. 4 (2014): 313–19. http://dx.doi.org/10.2754/avb201483040313.

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Routine methods for the evaluation of sperm quality are not sufficiently useful to determine the sensitivity of sperm cells to cold shock. The aim of our preliminary study was to determine whether the sperm plasma membrane integrity evaluated by modified hypoosmotic swelling test based on simple hypoosmotic swelling test (HOS test) and eosin-nigrosin staining could be helpful in predicting the degree of boar sperm survivability during semen cryopreservation. Ejaculates collected from 24 boars and 20 bulls were used in the experiment. Fresh ejaculates were evaluated by routine sperm analysis and a modified HOS test, and subsequently frozen. Sperm cryosurvivability was defined as the percentage of motile spermatozoa that survived the freezing process. A higher percentage of sperm was recovered after the thawing of semen with a higher percentage of HOS-positive and eosin-negative sperm (P < 0.01). Both indicators were found to be correlated (r = 0.707 and r = 0.705, respectively; P < 0.01). Moreover, the percentage of HOS-positive and eosin-negative sperm was similar to the percentage of viable sperm after thawing as determined by traditional eosin-nigrosin staining in boars (50.90 ± 9.88% and 49.31 ± 11.63%, respectively) and bulls (55.91 ± 10.34% and 55.63 ± 6.64%, respectively) and both indicators showed a positive correlation (r = 0.558 and r = 0.504, respectively; P < 0.05). In conclusion, based on the obtained results, we can assume that the modified HOS test can detect differences in sperm membrane resistance which allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation.
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Ani, N. V., T. P. Ogundunmade, D. Daniel, K. A. Raheem, E. O. Odirichukwu, and U. I. Osuagwuh. "Comparative Durability of Common Stains Used for Exfoliative Vaginal ‎Cytology." Sahel Journal of Veterinary Sciences 18, no. 2 (2021): 9–16. http://dx.doi.org/10.54058/saheljvs.v18i2.220.

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In a study to compare the durability of commonly used stains (Giemsa, Leishman, Wright, Eosin, Nigrosin and Gentian violet) for exfoliative vaginal cytology, vaginal smear was obtained from eleven apparently healthy West African Dwarf (WAD) female Goats and processed according to standard technique. Scores (0-3) were given on four parameters namely background of smears, overall staining pattern, cytoplasmic staining and nuclear staining. Quality index one (QI-I) was calculated from the ratio of score achieved to the maximum score possible (12), immediately post staining while quality index–II (QI-II) was obtained 35 days after. Calculation for durability index (DI) was self-derived and equalled to ratio of QI-II to QI-I. The data were presented as mean ± SEM. Multinomial logistic regression model was generated for the QI-I and QI-II using durability index as reference category. Giemsa, Leishman and Wright stains were more durable than others with their mean DI values significantly (P < 0.05) higher than Gentian violet, Nigrosin and Eosin.The model showed 89.2% overall model accuracy for the multinomial logistic regression model and 81.5% for the multinomial Bayes Naïve regression model. In conclusion, Giemsa, Leishman and Wright stains were more reliable and durable for exfoliative vaginal cytology compared to the other stains.
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Björndahl, Lars, Inger Söderlund, Sofia Johansson, Majid Mohammadieh, Mohammad Reza Pourian, and Ulrik Kvist. "Why the WHO Recommendations for Eosin-Nigrosin Staining Techniques for Human Sperm Vitality Assessment Must Change." Journal of Andrology 25, no. 5 (2004): 671–78. http://dx.doi.org/10.1002/j.1939-4640.2004.tb02839.x.

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Dissertations / Theses on the topic "Eosin-nigrosin staining"

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Foster, Mary L. "Comparison of Methods for Assessing Viability of Equine Spermatozoa and Effects of Seminal Plasma on Viability and Motion Characteristics of Equine Spermatozoa." 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7469.

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Assessment of sperm viability is an important component for evaluating stallion sperm quality. The flow cytometer is considered the standard in the assessment of sperm plasma membrane integrity (viability); however, this instrument is costly to purchase and use, and it requires an experienced technician to operate it. The growing practice of assisted reproductive technologies (ARTs) in the equine industry has increased the need for an accurate but cost-effective means of determining sperm membrane viability. The NucleoCounter® SP-100TM is reported to be an accurate, easy-to-perform, and an efficient stallion-side test for sperm membrane viability. To evaluate usefulness of the NucleoCounter® SP-100TM for assessing sperm membrane integrity, neat semen was subjected to four treatments with varying seminal plasma volumes and sperm concentrations. Sperm membrane viability was assessed immediately, and at 24 and 48 hours after cooled-storage using three methods: 1) flow cytometer utilizing the fluorescent vital stains SYBR-14/propidium iodide; 2) NucleoCounter® SP-100TM utilizing the fluorescent vital stain propidium iodide; 3) eosin-nigrosin stained air-dried smears of semen. Sperm motion characteristics (total and progressive motility) were assessed using a computer assisted sperm motion analyzer (CASMA) and results were compared to sperm membrane viability to determine the relationship between sperm membrane viability and motion characteristics. Results were compared statistically by: 1) analysis of variance (ANOVA); 2) linear regression analysis; 3) coefficient of variation on untransformed and transformed data (arc sine square root); and 3) the agreement of two instruments, by means of which the difference between measurements of the two instruments were plotted on the y-axis and the average of measurements from the two instruments were plotted on the x-axis. Results obtained with the NucleoCounter® SP-100TM agreed best with the flow cytometer, and least with eosin-nigrosin staining. Coefficients of variation were ≤ 5% for the three methods (transformed data). Sperm motion characteristics and sperm viability were similar among treatments at Time 0. At Times 24 and 48, sperm motion characteristics decreased at a more significant rate compared to viability in the treatments containing ≥ 50% seminal plasma, whereas differences among treatments were only significant at seminal plasma concentrations above 50% when only sperm membrane viability was considered.
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Book chapters on the topic "Eosin-nigrosin staining"

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Agarwal, Ashok, Sajal Gupta, and Rakesh Sharma. "Eosin-Nigrosin Staining Procedure." In Andrological Evaluation of Male Infertility. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-26797-5_8.

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Cecere, Julie T. "Eosin-Nigrosin Staining in the Evaluation of Sperm." In Equine Reproductive Procedures. John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118904398.ch114.

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