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Journal articles on the topic 'Ephedra extract'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Szymborska, Karolina, Rafał Frański, Błażej Gierczyk, and Monika Beszterda-Buszczak. "Extremely Rare Flavonoid Glycosides Identified in the Stems of Ephedra gerardiana by HPLC-MS and Their Antioxidant Activity." International Journal of Molecular Sciences 26, no. 7 (2025): 3097. https://doi.org/10.3390/ijms26073097.

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The plants of the genus Ephedra are mainly known for the contents of alkaloids; however, it has recently been found that they also contain phenolic constituents that show interesting bioactivities, e.g., antioxidative or antimicrobial. From among the Ephedrae herba, the Ephedra gerardiana seems to be relatively poorly researched in terms of flavonoid presence. In this study, on the basis of the results of high-pressure liquid chromatography–mass spectrometry (HPLC-MS) with cone voltage-induced fragmentation analysis, which are discussed in detail, the flavonoid glycosides present in Ephedra ge

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Sanjana, Walwante* Nandkishor Deshmukh Dr. Swati Deshmukh. "Formulation And Evaluation of Acne Gel Using Ephedra Extract." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 2361–68. https://doi.org/10.5281/zenodo.15415798.

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Ephedra  Herba (Ephedra), known as “MaHuang” in China, is the dried straw stem . At present, more than 60 species of Ephedra plants have been identified, which contain more than 100 compounds, including alkaloids, flavonoids, tannins, sugars, and organic phenolic acids. This herb has long been used to treat , skin disease, and other diseases, and has shown unique efficacy in the treatment of COVID-19 infection. Because alkaloids are the main components causing toxicity, the safety of Ephedra must be considered. However, the nonalkaloid components of Ephedra can be effectively

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Hajleh, Maha N. Abu, Khaled M. Khleifat, Moath Alqaraleh, et al. "Antioxidant and Antihyperglycemic Effects of Ephedra foeminea Aqueous Extract in Streptozotocin-Induced Diabetic Rats." Nutrients 14, no. 11 (2022): 2338. http://dx.doi.org/10.3390/nu14112338.

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Background: Ephedra foeminea is known in Jordan as Alanda and traditionally. It is used to treat respiratory symptoms such as asthma and skin rashes as an infusion in boiling water. The purpose of this study was to determine the antidiabetic property of Ephedra foeminea aqueous extract in streptozotocin-induced diabetic rats. Methods: The aqueous extract of Ephedra foeminea plant was used to determine the potential of its efficacy in the treatment of diabetes, and this extract was tested on diabetic rats as a model. The chemical composition of Ephedra foeminea aqueous extract was determined us

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Suhera, Aburawi. "Effect of Sub Toxic Dose of Ephedra Altissima Methanolic Extract on Reproductive System of Male Albino Mice." AlQalam Journal of Medical and Applied Sciences 3, no. 1 (2020): 13–22. https://doi.org/10.5281/zenodo.3929897.

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Background and objectives: Ephedra is nonflowering plants belonging to Ephedraceae family. There is little information available concerning toxicity. The aim of this work is to carry out phytochemical screening, sperm parameters, and histological study. Materials and Methods: Fresh aerial parts of Ephedra collected, identified, then air-dried. Phytochemical screening of methanolic extract of Ephedra powder using standard procedures were carried out. Twelve male albino mice (25-30 g), were divided into two groups. Mice were treated (i.p.) for 14 days with 1% T80 (5mL/kg), and extract (3000mg/kg

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Salima, Seddiki Lamia, Attoui Naouel, Aichaoui Fatiha, and Terich Chrifa Nour El Houda. "Effect of Ephedra alanda Extract against Alternaria alternata." Journal of Biomedical Research & Environmental Sciences 4, no. 1 (2023): 098–103. http://dx.doi.org/10.37871/jbres1653.

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Background: Some fungi have double host attacks for animals and plants, among them Alternaria alternata. Using a local medicinal plant such as Ephedra alanda is one way to fight its spreading. Aim: Evaluate the effect of the different extracts of Ephedra alanda against the mold Alternaria alternata. Material and Methods: This work used a different solvent by successive extraction of Ephedra alanda (Hexane, Chloroform, Ethyl acetate, and Methanol) and total alkaloids to evaluate the Anti- Alternaria alternata growth, besides assessing radical scavenging activity used the DPPH. Result: The resul

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Seddiki, Lamia Salima. "Eff ect of Ephedra alanda Extract against Alternaria alternata." J Biomed Res Environ Sci 4, no. 1 (2023): 098–103. https://doi.org/10.37871/jbres1653.

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<strong>Background: </strong>Some fungi have double host attacks for animals and plants, among them Alternaria alternata. Using a local medicinal plant such as Ephedra alanda is one way to fight its spreading. <strong>Aim: </strong>Evaluate the effect of the different extracts of Ephedra alanda against the mold Alternaria alternata. <strong>Material and Methods: </strong>This work used a different solvent by successive extraction of Ephedra alanda (Hexane, Chloroform, Ethyl acetate, and Methanol) and total alkaloids to evaluate the Anti- Alternaria alternata growth, besides asse

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Al-Trad, Bahaa, Mahmoud A Al –Qudah, Mazhar Al Zoubi, et al. "In-vitro and in-vivo Antioxidant Activity of the Butanolic Extract from the Stem of Ephedra alte." Biomedical and Pharmacology Journal 11, no. 3 (2018): 1239–45. http://dx.doi.org/10.13005/bpj/1485.

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Previous studies indicated that the extracts from different Ephedra species have antibacterial, antifungal and antioxidant activities. However, none of the published report described the phytochemical components and the antioxidant capacities of Ephedra alte belonging to the family Ephedraceae. To evaluate the in-vitro and in-vivo antioxidant activities of the butanolic extract from stems of Ephedra alte from northern Jordan. Graded concentrations of butanolic extracts from stems of E. alte plant were subjected to four different in-vitro antioxidant assays (DPPH, ABTS, ferrous ion chelating an

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8

Sioud, Fairouz, Aida Lahmer, Mouna Selmi, Fadwa Chaabane, and Leila Chekir-Ghedira. "Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo." Asian Pacific Journal of Tropical Biomedicine 14, no. 4 (2024): 154–61. http://dx.doi.org/10.4103/apjtb.apjtb_2_24.

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Objective: To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in vivo. Methods: The effects of the methanolic extract of Ephedra alata on the viability, migration as well as apoptosis of breast cancer 4T1 cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay, and annexin V-FITC staining assay, respectively. Histological examination was also carried out. Moreover, a murine breast cancer model was established to evaluate the inhibitory effect of the extract. Biochem

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Danciu, Corina, Delia Muntean, Ersilia Alexa, et al. "Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line." Molecules 24, no. 1 (2018): 13. http://dx.doi.org/10.3390/molecules24010013.

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Ephedra alata Decne. belongs to the Ephedraceae family. It is a species of Ephedra that grows mostly in the desert. Today, the main importance of Ephedra species in the medical field is due to the presence of the alkaloids derived from phenyl-alanine, which act on the sympathetic nervous system as a sympathomimetic. The aim of this study was to conduct a phytochemical characterization of the hydroalcoholic extract of the aerial part of Ephedra alata Decne., which is indigenous to Tunis, that involves the total phenolic content, individual phenolic content, and antioxidant activity as well as a

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Wafa, Zouari Ahmed Meriem Chadou Wissal Ben Ghalia and Samir Derouiche. "Phytochemical Profile, Antioxidant and Anti-inflammatory Activities of Algerian Ephedra alata." Environmental Science Archives 3, no. 2 (2024): 59–70. https://doi.org/10.5281/zenodo.13742808.

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The aim of this study was to investigate phytochemicals, the antioxidant, anti-inflammatory, hemolysis assay of Ephedra alata Qualitative analysis of phytochemicals and quantitative analysis of total phenolics and flavonoids were prepared by using standard protocols. Qualitative phytochemical analysis revealed that the aqueous extract show richness in flavonoids, terpenoids, saponins, steroids, reducing compound, tannins, phenols and alkaloids. Total phenol and flavonoid content show highest concentration in aqueous extract of E. alata (68.009mg GA Eq/gm, 4mg QEq/gm). In vitro, the DPPH and FR

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Boussena, Abdelhadi abdelhadi, Fouad Bahri, Ahmed Bouyahyaoui, Mohamed Kouidri, and Malika Meziane. "SCREENING OF PHYTOCHEMICAL, EVALUATION OF PHENOLIC CONTENT, ANTIBACTERIAL AND ANTIOXYDANT ACTIVITIES OF EPHEDRA ALATA FROM THE ALGERIAN SAHARA." Journal of Applied Biological Sciences 16, no. 2 (2022): 220–29. https://doi.org/10.71336/jabs.999.

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Ephedra alata is a medicinal plant that has a long history of usage in folk medicine for the treatment of several diseases such as bronchial asthma, digestive system disorders and cancer. The present study aimed to elucidate the chemical constituents of the crude methanolic and hydro-methanolic extracts of Ephedra alata aerial part and to assess their antibacterial and antioxidant activities. In this study, the percentage yield of crude methanolic and hydro-methanolic extracts was 12.92% and 15.01% respectively. Phytochemical screening investigations using standard method showed that E. alata

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Kobayashi, Yoshinori. "Analgesic Effects and Side Effects of Ephedra Herb Extract and Ephedrine Alkaloids-free Ephedra Herb Extract." YAKUGAKU ZASSHI 137, no. 2 (2017): 187–94. http://dx.doi.org/10.1248/yakushi.16-00233-5.

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Abdelhadi, Boussena, Bahri Fouad, Bouyahyaoui Ahmed, Kouidri Mohamed, and Meziane Malika. "SCREENING OF PHYTOCHEMICAL, EVALUATION OF PHENOLIC CONTENT, ANTIBACTERIAL AND ANTIOXYDANT ACTIVITIES OF EPHEDRA ALATA FROM THE ALGERIAN SAHARA." Journal of Applied Biological Sciences 16, no. 2 (2022): 220–29. https://doi.org/10.5281/zenodo.6590258.

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<em>Ephedra alata </em>is<em> </em>a<strong> </strong>medicinal plant that has a long history of usage in folk medicine for the treatment of several diseases such as bronchial asthma, digestive system disorders and cancer. The present study aimed to elucidate the chemical constituents of the crude methanolic and hydro-methanolic extracts of <em>Ephedra alata</em> aerial part and to assess their antibacterial and antioxidant activities. In this study, the percentage yield of crude methanolic and hydro-methanolic extracts was 12.92% and 15.01% respectively. Phytochemical screening investigations

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14

Roman, Mark C., D. Gray, G. Luo, et al. "Determination of Ephedrine Alkaloids in Botanicals and Dietary Supplements by HPLC-UV: Collaborative Study." Journal of AOAC INTERNATIONAL 87, no. 1 (2004): 1–14. http://dx.doi.org/10.1093/jaoac/87.1.1.

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Abstract An international collaborative study was conducted of a high-performance liquid chromatography (HPLC)-UV method for the determination of the major (ephedrine [EP] and pseudoephedrine [PS]) and minor (norephedrine [NE], norpseudoephedrine [NP], methylephedrine [ME], and methylpseudoephedrine [MP]) alkaloids in selected dietary supplements representative of the commercially available products. Ten collaborating laboratories determined the ephedrine-type alkaloid content in 8 blind replicate samples. Five products contained ephedra ground herb or ephedra extract. These 5 products include

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15

Azal Hamoody Juma, Montadher Mahdi, Kawakeb Abdulla, and Yasser Dawood. "Evaluate the utility of Iraqi Ephedra Transitoria methyl extract on the proliferation of cervical cancer cell line." Iraqi Journal of Cancer and Medical Genetics 17, no. 2 (2024): 114–21. https://doi.org/10.29409/ijcmg.v17i2.370.

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Objective: This study aimed to assess the inhibitory effect of a methanolic extract of Iraqi Ephedra transitoria on the proliferation of cervical cancer cells.Materials and methods: To assess the anticancer properties of Ephedra transitoria, a HeLa cell line (derived from cervical cancer) was utilized. The concentration of the evaluated agent was varied between 0.1 and 1000 µg/ml, and the cells were incubated for 24 and 72 hours. The IC50 value was used to identify the potency of the examined substance.Results: The study results demonstrated that the extraction ratio of E. transitoria was 8%.

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Castro, Joaudimir, M. V. Balarama Krishna, and R. Kenneth Marcus. "Liquid Chromatography-Particle Beam Electron Ionization Mass Spectrometry Method for Analysis of Botanical Extracts: Evaluation of Ephedrine Alkaloids in Standard Reference Materials." Journal of AOAC INTERNATIONAL 93, no. 6 (2010): 1788–97. http://dx.doi.org/10.1093/jaoac/93.6.1788.

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Abstract The preliminary validation of a high-performance liquid chromatography particle beam mass spectrometry method (HPLC-PB/MS) with electron impact ionization source for analysis of botanical extracts is presented. The LC-PB/MS system was evaluated for the analysis of ephedrine alkaloids using ephedra-containing National Institute of Standards and Technology dietary supplement standard reference materials (SRMs) 3241 Ephedra Sinica Stapf Native Extract and 3242 Ephedra Sinica Stapf Commercial Extract. The ephedrine alkaloids were separated by reversed-phase chromatography using a phenyl c

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Khan, Aman, Gul Jan, Afsar Khan, Farzana Gul Jan, Ali Bahadur, and Muhammad Danish. "In Vitro Antioxidant and Antimicrobial Activities of Ephedra gerardiana (Root and Stem) Crude Extract and Fractions." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/4040254.

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The utilization of medicinal plants to treat infectious disease is a common practice in developing countries worldwide. The present study was aimed at evaluating the crude extracts of Ephedra gerardiana (root and stem) with different chemicals for antioxidant and antimicrobial (fungal and bacterial) potential. The results revealed that the ethyl acetate fractions of E. gerardiana (root and stem) have significant free radical scavenging potential with values 2.96±0.39 and 2.73±0.84 while n-butanol and aqueous fractions showed IC502.69±0.26 and 3.44±0.69 µg/ml in stem. Furthermore, crude extract

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Sharpless, Katherine E., David L. Anderson, Joseph M. Betz, et al. "Preparation and Characterization of a Suite of Ephedra-Containing Standard Reference Materials." Journal of AOAC INTERNATIONAL 89, no. 6 (2006): 1483–95. http://dx.doi.org/10.1093/jaoac/89.6.1483.

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Abstract The National Institute of Standards and Technology, the U.S. Food and Drug Administration, Center for Drug Evaluation and Research and Center for Food Safety and Applied Nutrition, and the National Institutes of Health, Office of Dietary Supplements, are collaborating to produce a series of Standard Reference Materials (SRMs) for dietary supplements. A suite of ephedra materials is the first in the series, and this paper describes the acquisition, preparation, and value assignment of these materials: SRMs 3240 Ephedra sinica Stapf Aerial Parts, 3241 E. sinica Stapf Native Extract, 324

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Bouafia, Waffa, Soumia Mouffouk, and Hamada Haba. "Quantification of total bioactive contents and evaluation of the antioxidant and antibacterial activities of crude extracts from Ephedra altissima Desf." Acta Scientiarum. Biological Sciences 43 (March 30, 2021): e52123. http://dx.doi.org/10.4025/actascibiolsci.v43i1.52123.

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The present work describes the in vitro biological activities of the crude extracts (petroleum ether, ethyl acetate and n-butanol) prepared from the species Ephedra altissima Desf. The estimation of total phenolic, flavonoid and tannin contents were carried out using the Folin-Ciocalteu, trichloroaluminum and acidified vanillin methods, respectively. The evaluation of the in vitro antioxidant activities were performed by three different methods namely: scavenging of the free radical ABTS, permanganate reducing antioxidant capacity, and potentiometric assay. In addition, the antibacterial activ

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El-Zayat, Mustafa Mohsen, Mostafa M. Eraqi, Hani Alrefai, et al. "The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract." Biomolecules 11, no. 3 (2021): 470. http://dx.doi.org/10.3390/biom11030470.

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The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of Ephedra aphylla as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and tannin contents were reduced in the case of the prepared samples of nanoparticles than the original values in the aqueous extract. The prepared extract of Ephedra aphylla and its selenium and zinc nanoparticles showed high potency as antioxidant agents as a result of the DPPH• assay. The samples were

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Nakamori, Shunsuke, Jun Takahashi, Sumiko Hyuga, et al. "Analgesic Effects of Ephedra Herb Extract, Ephedrine Alkaloids–Free Ephedra Herb Extract, Ephedrine, and Pseudoephedrine on Formalin-Induced Pain." Biological and Pharmaceutical Bulletin 42, no. 9 (2019): 1538–44. http://dx.doi.org/10.1248/bpb.b19-00260.

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Al-saraireh, Yousef M., Ahmed M. M. Youssef, Fatemah O. F. O. Alshammari, et al. "Phytochemical characterization and anti-cancer properties of extract of Ephedra foeminea (Ephedraceae) aerial parts." Tropical Journal of Pharmaceutical Research 20, no. 8 (2022): 1675–81. http://dx.doi.org/10.4314/tjpr.v20i8.18.

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Purpose: To evaluate the phytochemical profile of methanol extract of Ephedra foeminea and assess its anti-carcer effect on a large set of normal and cancerous cell lines. Methods: Extraction of air-dried powder of aerial parts of E. foeminea was carried out with methanol. The bioactive compounds in the extract were determined using gas chromatography/mass spectrometry (GC-MS). The anti-cancer effect of the extract was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay against various types of normal and cancer cell lines. Serial concentrations of plan

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Xu, Jiajia, Congfen He, and Rui Tian. "Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation." Current Issues in Molecular Biology 47, no. 1 (2025): 68. https://doi.org/10.3390/cimb47010068.

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Hair loss is one of the skin conditions that can affect people’s mental health. Plant raw material extracts are of great interest due to their safety. In this study, we utilize reverse network pharmacology to screen for key targets of the Wnt/β-catenin signaling pathway and the TGFβ/BMP signaling pathway, as well as key differential lipids, for plant raw materials selection. The aim is to identify plant raw materials that may have anti-hair loss properties and to validate these findings through cell experiments. Licorice, salvia miltiorrhiza, mulberry leaf, ephedra and curcumae radix were foun

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Cheng, Yezhe, Yu Zhang, Hang Xing, Kun Qian, Longshan Zhao, and Xiaohui Chen. "Comparative Pharmacokinetics and Bioavailability of Three Ephedrines in Rat after Oral Administration of Unprocessed and Honey-Fried Ephedra Extract by Response Surface Experimental Design." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/2802193.

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Ephedra have been used as a common traditional Chinese medicine for thousands of years. However, the perspiration effect of the unprocessed ephedra was too strong. Clinical trials have shown that processing methods play a critical role in moderating the perspiration property of ephedra according to the needs. A LC-MS/MS method was developed and validated to compare the pharmacokinetic properties of the three ephedrines after oral administration of unprocessed and honey-fried ephedra extract. The contents of honey, frying temperature, and frying time were set at 20%, 116°C, and 7 min by the Box

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Hassan, Athraa AbdulJabbar, and LH Ali. "Therapeutic Potential of Ephedra alata Alcoholic Extract Against Lead Acetate-Induced Nephrotoxicity in Male Albino Rats: Biochemical, Histological, and Antioxidant Insights." Babcock University Medical Journal 8, no. 1 (2025): 291–305. https://doi.org/10.38029/babcockuniv.med.j..v8i1.828.

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Objective: This study investigated the nephroprotective potential of Ephedra alata alcoholic extract against lead acetate-induced toxicity in rats. Methods: Forty male albino rats were assigned to five groups: control, lead acetate only, E. alata only, therapeutic (lead + E. alata), and preventive (E. alata + lead). Kidney function, oxidative stress, cytokine levels, and tissue morphology were evaluated. Results: Lead acetate significantly increased serum urea, creatinine, MDA, IL-1β, and TNF-α levels (p<0.05), while reducing GPx. Co-administration of E. alata reversed these alterations. Hi

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Amakura, Yoshiaki, Morio Yoshimura, Saori Yamakami, et al. "Characterization of Phenolic Constituents from Ephedra Herb Extract." Molecules 18, no. 5 (2013): 5326–34. http://dx.doi.org/10.3390/molecules18055326.

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Amakura, Yoshiaki. "Characterization of Phenolic Constituents from Ephedra Herb Extract." YAKUGAKU ZASSHI 137, no. 2 (2017): 167–71. http://dx.doi.org/10.1248/yakushi.16-00233-2.

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Yoshimura, Morio, Yoshiaki Amakura, Sumiko Hyuga, et al. "Quality Evaluation and Characterization of Fractions with Biological Activity from Ephedra Herb Extract and Ephedrine Alkaloids-Free Ephedra Herb Extract." Chemical and Pharmaceutical Bulletin 68, no. 2 (2020): 140–49. http://dx.doi.org/10.1248/cpb.c19-00761.

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Askar, Mahmood Razzaq Mashar. "Bacterial responses to Ephedra aphylla stem extract and green-synthesized Ag-TiO₂ and Ag-SeO₂ core/shell nanocomposites: unveiling antimicrobial and antioxidant properties." RSC Advances 15, no. 17 (2025): 13152–71. https://doi.org/10.1039/d5ra00936g.

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Yang, Lin, Wei Juan Li, Jing Long, et al. "Isolation of Endophytic Fungi from Ephedra intermedia and Research Antibacterial Activity of Secondary Metabolite Produced by the Fungi." Advanced Materials Research 881-883 (January 2014): 488–92. http://dx.doi.org/10.4028/www.scientific.net/amr.881-883.488.

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20 strains of endophytic fungi were isolated from roots and stems of Ephedra intermedia. After fermentation these strains, fermentation broth and mycelium were extracted by ethyl acetate, n-butanol and methyl alcohol, producing 60 extracts. Then the antibacterial activity of these extracts was researched, the result found that 33 extracted samples could evidently inhibit the growth of six different bacteria, accounted for 55%. The MIC of ethyl acetate extract of J109 was 0.15625mg/mL to five test bacterial expect Streptococcus uberi.

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Muna Hasson Saoudi. "Evaluation of Phytochemical, proximate analysis, mineral composition and polyphenolic contents of aqueous extract of (Ephedra (Alata) leaves." Journal of Pharmaceutical Negative Results 13, no. 4 (2022): 715–19. http://dx.doi.org/10.47750/pnr.2022.13.04.096.

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This study was conducted to determine the mineral, phytochemical constituents and nutritive worth of (leaf Ephedra )which grows in AL –Salman desert, south of Iraq. The nutritional properties and elemental values of this (medicinal plant) were evaluated by leaves aqueous extract that showed high amounts of proteins 12% and carbohydrates 41%, with low amounts of fats. The Results of the phyto-composition in mg/100 g ((DW)) revealed variable contents of TPC, TFC and (TAC )in Ephedra extract leaves obtaining a yield of (48.15±0.6,33.3±0.1,8.8±0.5) respectively. The mineral investigation indicated

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Lee, Doseung, and Dong-Sun Lee. "Antiviral activity of methanol extract from Ephedra sinica Stapf." Korean Journal of Food Preservation 21, no. 5 (2014): 735–39. http://dx.doi.org/10.11002/kjfp.2014.21.5.735.

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Nam, Nguyen-Hai, Chang-Woo Lee, Dong-Ho Hong, Hwan-Mook Kim, Ki-Hwan Bae, and Byung-Zun Ahn. "Antiinvasive, antiangiogenic and antitumour activity of Ephedra sinica extract." Phytotherapy Research 17, no. 1 (2003): 70–76. http://dx.doi.org/10.1002/ptr.901.

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Odaguchi, Hiroshi, Sumiko Hyuga, Mariko Sekine, et al. "Safety and Efficacy of Ephedrine Alkaloids-Free Ephedra Herb Extract (EFE) for Mild COVID-19: A Double-Blind, Placebo-Controlled, Randomized Comparative Trial." Microorganisms 13, no. 3 (2025): 641. https://doi.org/10.3390/microorganisms13030641.

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Several Ephedra Herb-containing Kampo medicines are common initial treatments for various infections; however, the ephedrine alkaloids in Ephedra Herb can cause side effects by stimulating adrenergic receptors. Accordingly, an ephedrine alkaloids-free Ephedra Herb Extract (EFE) has been developed. This study aimed to evaluate whether EFE can be used effectively and safely in patients with mild coronavirus disease 2019 (COVID-19). We randomized patients with mild COVID-19 to receive EFE equivalent to 6 g of Ephedra Herb per day or a placebo for 14 days. The primary efficacy endpoint was the non

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Bennici, Giulia, Inas Al Younis, Abeer Sharfalddin, et al. "Medicinal effects of Ephedra foeminea aqueous extracts: Metabolomic characterization, biological evaluation, and molecular docking." PLOS One 20, no. 7 (2025): e0328995. https://doi.org/10.1371/journal.pone.0328995.

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Ephedra foeminea Forssk. is a medicinal plant traditionally used across various cultures and recognized for its historical significance in herbal medicine. It has been used as an herbal infusion to treat multiple respiratory diseases, headaches, and nasal congestion, as well as to prevent and manage breast cancer. In order to evaluate the validity of this ancestral knowledge, we aimed to investigate hot aqueous extracts of E. foeminea branches and fruits firstly by using untargeted metabolomic analysis, characterizing the chemical profiles of hot aqueous extracts from E. foeminea branches and

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Mimi, Nassiba, Leila Madani, Djamila Kerrouche, et al. "Comparison of Antioxidant and Antibacterial Properties of Five Plants with Anti-Diabetes and Anti-Cancer Potential." Microbiology Research 16, no. 6 (2025): 108. https://doi.org/10.3390/microbiolres16060108.

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Polyphenols and flavonoids are bioactive organic compounds extracted from medicinal plants. They exhibit significant antioxidant and antibacterial properties, which help fight several chronic diseases, such as diabetes and cancer. Numerous therapeutic effects and a broad spectrum of biological activities are exhibited by the following five medicinal plants traditionally utilized in medicine for the treatment of diabetes and cancer: Ginger, ephedra alata, ajuga iva, nettle, and graviola (annona muricata). The objective of the present study is to examine ethanolic and aqueous extracts exhaustive

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Odaguchi, Hiroshi, Sumiko Hyuga, Mariko Sekine, et al. "The Adverse Effects of Ephedra Herb and the Safety of Ephedrine Alkaloids-free Ephedra Herb Extract (EFE)." YAKUGAKU ZASSHI 139, no. 11 (2019): 1417–25. http://dx.doi.org/10.1248/yakushi.19-00122.

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Omar, Ghadeer, Lubna Abdallah, Aseel Sawafta, Huda Bani-Odeh, Jamil El Araj, and Osaid Marie. "Cytostatic potential of Ephedra aphylla Forssk. and Ephedra foeminea Forssk. different extract types against HeLa cell line." Journal of Medicinal Plants Studies 12, no. 4 (2024): 333–40. http://dx.doi.org/10.22271/plants.2024.v12.i4d.1726.

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Uema, Masashi, Masashi Hyuga, Kenzo Yonemitsu, et al. "Antiviral Effect of Ephedrine Alkaloids-Free Ephedra Herb Extract against SARS-CoV-2 In Vitro." Microorganisms 11, no. 2 (2023): 534. http://dx.doi.org/10.3390/microorganisms11020534.

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We report for the first time that ephedrine alkaloids-free Ephedra Herb extract (EFE) directly inhibits the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro and that the addition of EFE to the culture medium before viral infection reduces virus titers in the culture supernatant of SARS-CoV-2, including those of variant strains, by more than 99%, 24 h after infection. The addition of Ephedra Herb macromolecule condensed-tannin, which is the main active ingredient responsible for the anticancer, pain suppression, and anti-influenza effects of EFE, similarly su

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Al-Nemi, Ruba, Arwa A. Makki, Khaled Sawalha, Dina Hajjar, and Mariusz Jaremko. "Untargeted Metabolomic Profiling and Antioxidant Capacities of Different Solvent Crude Extracts of Ephedra foeminea." Metabolites 12, no. 5 (2022): 451. http://dx.doi.org/10.3390/metabo12050451.

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Ephedra foeminea is a traditional medicinal plant used in the Eastern Mediterranean region. This study aims to investigate the chemical profiles of different solvent extracts of E. foeminea via an untargeted metabolomics approach, alongside determining their antioxidant capacities. E. foeminea samples collected from Jordan were macerated in solvents of varying polarities; dichloromethane/methanol, methanol, ethanol, ethyl acetate, and acetone. The crude extracts were subjected to comprehensive chemical profiling and metabolomics study using Gas chromatography–Mass spectrometry (GC–MS), Liquid

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Khanal, Lekha Nath, Khaga Raj Sharma, Yuba Raj Pokharel, and Surya Kant Kalauni. "Characterization of Essential Oil, Estimation of Phenolic and Flavonod Content and Biological Activities of Ephedra pachyclada BOISS." Journal of Institute of Science and Technology 27, no. 1 (2022): 27–35. http://dx.doi.org/10.3126/jist.v27i1.40590.

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The northern areas of Nepal are rich in biodiversity and contain a large number of medicinal plant species including the Genus Ephedra of evergreen gymnosperm, belonging to the family Ephedraceae. The plants have been used by the peoples of the Himalayan region for the treatment of asthma, blood pressure, and gastritis for many years. This study was aimed for the evaluation of phytochemical, antioxidant, and antimicrobial activities of methanol, ethyl acetate, dichloromethane, and n-hexane extracts, and gas chromatography-mass spectrometry (GCMS) profiling of the essential oil of the aerial pa

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Cheriti, Abdelkrim, Noureddine Gherraf, Elyacout Chebouat, Belkhir Dadamoussa, Samer Gharabli, and Messaouda Allaoui. "Acid Content of the Dichloromethane Extract of Ephedra Alata Leaves." حوليات العلوم و التكنولوجيا 6, no. 1 (2014): 40–43. http://dx.doi.org/10.12816/0010625.

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Nishi, Akinori, Sumiko Hyuga, Masashi Hyuga, et al. "The Antiviral Effect of Ephedrine Alkaloids-Free Ephedra Herb Extract, EFE, on Murine Coronavirus Growth in the Lung and Liver of Infected Mice." Microorganisms 13, no. 4 (2025): 830. https://doi.org/10.3390/microorganisms13040830.

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Ephedrine alkaloids-free Ephedra Herb extract (EFE) was developed to reduce the adverse effects of Ephedra Herb, a constituent drug in Kampo medicines. It is produced by decocting Ephedra Herb with hot water and excluding the ephedrine alkaloids. EFE has analgesic and anti-cancer effects and inhibits respiratory viruses in vitro. To assess the pharmacological action of EFE in vivo, we evaluated its effect on the replication of murine hepatitis virus (MHV), a coronavirus that causes hepatitis, pneumonia, and severe acute respiratory syndrome-like symptoms, within infected mice. On Day 0, MHV wa

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Hyuga, Sumiko, Masashi Hyuga, Naohiro Oshima, et al. "Ephedrine alkaloids-free Ephedra Herb extract: a safer alternative to ephedra with comparable analgesic, anticancer, and anti-influenza activities." Journal of Natural Medicines 70, no. 3 (2016): 571–83. http://dx.doi.org/10.1007/s11418-016-0979-z.

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YOUNES, Kareem M., Fawaz ALHEIBSHY, Amal M. ALRASHIEDI, et al. "Insight into possible therapeutic applications of Ephedra transitoria: in-vitro biological, toxicological activities and GC-MS analysis of aerial parts’ extract." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 52, no. 1 (2024): 13442. http://dx.doi.org/10.15835/nbha52113442.

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Most Arab nations have arid regions where Ephedra transitoria, a plant, flourishes. It has a variety of medical benefits. It belongs to the Ephedraceae family. The plant's aerial portions were procured from the Hail region of Saudi Arabia, methanol was used to extract bioactive phytochemicals with variable solubility in an efficient manner, and the resulting extract was tested on several cancer cell lines. The extract's cytotoxic potential was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay method against standard vinblastine sulfate and for each cell l

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Nasar, Khalil, Ali, Shah, Ayaz, and Shinwari. "Phytochemical Analysis, Ephedra Procera C. A. Mey. Mediated Green Synthesis of Silver Nanoparticles, Their Cytotoxic and Antimicrobial Potentials." Medicina 55, no. 7 (2019): 369. http://dx.doi.org/10.3390/medicina55070369.

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: Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) w

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Odaguchi, Hiroshi, Mariko Sekine, Sumiko Hyuga, et al. "A Double-Blind, Randomized, Crossover Comparative Study for Evaluating the Clinical Safety of Ephedrine Alkaloids-Free Ephedra Herb Extract (EFE)." Evidence-Based Complementary and Alternative Medicine 2018 (August 5, 2018): 1–8. http://dx.doi.org/10.1155/2018/4625358.

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Ephedra Herb is an important crude drug; it is used in various Traditional Japanese Medicine (Kampo) formulations. Its significant pharmacological effects have been believed to be attributed to ephedrine and pseudoephedrine, which sometimes induce adverse effects. On the other hand, it has been reported that some of these pharmacological effects are not dependent on ephedrine or pseudoephedrine. Ephedrine alkaloids-free Ephedra Herb extract has been newly developed. It has been reported to have analgesic, anti-influenza, and antimetastatic effects. This clinical trial was aimed at verifying th

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Singh, Mahabir, Minakshi Rajput, Kuldeep Yadav, and Narender Singh. "Evaluation of antimicrobial activity and phytochemical qualitative analysis of Ephedra foliata Boiss. ex C.A. Mey." Herba Polonica 68, no. 2 (2022): 70–75. http://dx.doi.org/10.2478/hepo-2022-0007.

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Summary Introduction: Ephedra foliata Boiss. ex C.A. Mey of the Ephedraceae family is an evergreen shrub distributed throughout North Africa and Southwest Asia. It has been a characteristic source of alkaloids like ephedrine, pseudoephedrine and other related mixes, which are of great importance for their biological and pharmacological potential. Objective: This study is aimed to evaluate the antimicrobial potential and phytochemical constituents studies on stem, leaves and flowers extracts of E. foliata. Method: The air-dried plant sample was powdered with an electric grinder, then extracted

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Mpingirika, Eric Zadok, Ahmed El Hosseiny, Sheri Magdy Saleeb Bakheit, Rami Arafeh, and Asma Amleh. "Potential Anticancer Activity of Crude Ethanol, Ethyl Acetate, and Water Extracts of Ephedra foeminea on Human Osteosarcoma U2OS Cell Viability and Migration." BioMed Research International 2020 (July 9, 2020): 1–16. http://dx.doi.org/10.1155/2020/3837693.

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Medicinal plants are potential sources for a wide range of complex compounds with probable anticancer activity. Ephedra foeminea Forssk. (E. foeminea), a medicinal plant found in the Eastern Mediterranean, has recently been gaining popularity as a cancer remedy; there is, however, a paucity of empirical evidence supporting this claim. In this study, the effect of E. foeminea ethyl acetate, ethanol, and water crude extracts on viability, migratory ability, and the steady-state mRNA levels of genes involved in these processes was, respectively, examined using MTT assay, wound healing assay, and

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Matsuo, Yukiko, Mayu Sasaki, Haruhiko Fukaya та ін. "Chemical Constituents of the Terrestrial Stems of Ephedra sinica and their PPAR-γ Ligand-Binding Activity". Planta Medica International Open 7, № 01 (2020): e12-e16. http://dx.doi.org/10.1055/a-1094-9229.

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AbstractBioassay-guided fractionation of the MeOH extract of Ephedra sinica terrestrial stems, using a PPAR-γ ligand binding assay, resulted in the isolation of 10 compounds, including one new bisabolane-type sesquiterpenoid (10). The structure of the new compound was determined by extensive spectroscopic analysis, including two-dimensional (2D) NMR. Among the isolated compounds, the sitosterol derivatives (1 and 2), flavonoid glucoside (7), and the new sesquiterpenoid (10), showed significant PPAR-γ ligand-binding activity.

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