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Journal articles on the topic 'Epididymis tail'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Matson, P. L., P. A. Eden, S. D. Vitali, C. A. Lawrence, and H. M. Robertson. "222. Sperm morphology within the testis and cauda epididymis of a Tasmanian devil (Sarcophilus harrisii)." Reproduction, Fertility and Development 17, no. 9 (2005): 86. http://dx.doi.org/10.1071/srb05abs222.

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A male Tasmanian devil, aged 6.5 years, was euthanased with intravenous pentobarbitone overdose due to the diagnosis of squamous cell carcinoma. The testes and cauda epididymes were removed immediately after euthanasia and placed into TALP-HEPES medium containing 3% BSA and dissected. Smears of the isolated sperm were made, air-dried and stained using Diff-Quik. Sperm were classified using a modification of a system originally applied to the brush-tailed possum1 as follows: (a) Immature I – head is perpendicular or greater than 45° to the tail.(b) Immature II – head forms an acute angle of 45°

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Tomomasa, H., S. Waguri, T. Umeda, K. Koiso, E. Kominami, and Y. Uchiyama. "Lysosomal cysteine proteinases in rat epididymis." Journal of Histochemistry & Cytochemistry 42, no. 3 (1994): 417–25. http://dx.doi.org/10.1177/42.3.8308258.

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To examine the precise localization of lysosomal cysteine proteinases, cathepsins B, H, and L in rat epididymal epithelial cells, immunohistochemistry and enzyme assay were applied to the epididymal tissue. Granular immunodeposits for cathepsins B and H were detected in epididymal epithelial cells, whereas faint or no immunoreactivity for cathepsin L was found. Moreover, immunoreactivity for cathepsin B appeared mainly in principal cells and was more intense in the head of the epididymis than in the tail, whereas that for cathepsin H appeared in both principal and clear cells and was more inte

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3

Wysokińska, Anna, Ewa Wójcik, and Angelika Chłopik. "Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods." Animals 11, no. 1 (2021): 227. http://dx.doi.org/10.3390/ani11010227.

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Evaluation of sperm morphometry is an important criterion in the diagnosis of a male animal’s suitability for breeding. The aim of the study was to evaluate the morphometry of sperm from the epididymides of dogs subjected to routine castration using various staining methods. The study was carried out on semen collected from ten healthy dogs. Gonads were obtained from each dog during routine castration at a veterinary surgery. Then, the epididymides (caput, corpus, cauda) were isolated from the gonads, semen was collected from them and microscope slides were prepared. The slides for evaluation

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Angrimani, D. S. R., C. F. Lúcio, J. D. A. Losano, et al. "The influence of canine brucellosis on morphofunctional features of epididymal spermatozoa: case report." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 68, no. 6 (2016): 1449–52. http://dx.doi.org/10.1590/1678-4162-9015.

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ABSTRACT The present work reports a clinical case of a mongrel dog, with serological diagnosis of brucellosis, from which epididymal sperm analysis was performed. Sperm samples were collected from different segments of the epididymis (tail, corpus, and caput). Sperm samples were evaluated for computer-assisted motility analysis (CASA), spermatic morphology, mitochondrial activity and sperm plasmatic membrane and acrosomal integrity. Changes in sperm movement patterns were found (progressive motility, percentage of rapid sperm, percentage of rapid velocity, average pathway, curvilinear velocity

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Gloria, Alessia, Alberto Contri, Elena Mele, Silvia Fasano, Riccardo Pierantoni, and Rosaria Meccariello. "Kisspeptin Receptor on the Sperm Surface Reflects Epididymal Maturation in the Dog." International Journal of Molecular Sciences 22, no. 18 (2021): 10120. http://dx.doi.org/10.3390/ijms221810120.

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Alongside the well-known central modulatory role, the Kisspeptin system, comprising Kiss1, its cleavage products (Kisspeptins), and Kisspeptin receptor (Kiss1R), was found to regulate gonadal functions in vertebrates; however, its functional role in the male gamete and its localization during maturation have been poorly understood. The present study analyzed Kisspeptin system in dog testis and spermatozoa recovered from different segments of the epididymis, with focus on Kiss1R on sperm surface alongside the maturation during epididymal transit, demonstrated by modification in sperm kinetic, m

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Cupperová, P., M. Simon, J. Antalíková, K. Michalková, Ľ. Horovská, and S. Hluchý. "Distribution of tetraspanin family protein CD9 in bull reproductive system." Czech Journal of Animal Science 59, No. 3 (2014): 134–39. http://dx.doi.org/10.17221/7293-cjas.

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The CD9 cell surface molecule has been found to be important for the fertilization process of mammals. The aim of this study was to investigate, whether the molecule CD9 is expressed on bull sperm during the spermatogenesis and maturation of spermatozoa as well as in bull reproductive organs and their secreta. The expression of bovine CD9 was examined by immunohistochemistry, immunofluorescence, and immunoblotting. The histochemical studies using an anti-CD9 monoclonal antibody showed strong staining in the myeloid and collagenous tissue layer of testis and epididymis. Strong reaction was obse

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Patoulias, Ioannis, Christos Kaselas, Dimitrios Patoulias, et al. "Epididymal Adenomatoid Tumor: A Very Rare Paratesticular Tumor of Childhood." Case Reports in Medicine 2016 (2016): 1–4. http://dx.doi.org/10.1155/2016/9539378.

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Adenomatoid tumor is an uncommon benign mesothelial neoplasm, usually localized in the epididymis. It is the most common paratesticular tumor of middle-aged patients (average age of clinical presentation: 36 years). However, these tumors in pediatric and pubertal patients are extremely rare. Due to their rarity, we present a case of adenomatoid tumor of the tail of the epididymis in a 16-year-old patient. After systematic research of the current literature, we did not find another case report of epididymal adenomatoid tumor in a male patient aged 16 years old or less. This notice and our conce

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Zvyagina, V. I., E. S. Bel’skikh, D. V. Medvedev, and N. A. Golovach. "Studying the functional condition of rat epididymis mitochondria at nitric oxide (II) synthesis change." Kazan medical journal 96, no. 5 (2015): 814–18. http://dx.doi.org/10.17750/kmj2015-814.

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Aim. To study the levels of nitric oxide (II) metabolites, lactate, endogenic carnitine and mitochondrial oxidoreductase activity in epididymis tissue at nitric oxide low synthesis and, based on the obtained data, to assess the functional condition of rat epididymis mitochondria. Methods. 16 Wistar rats were allocated to two equal groups: The first group was administered L-NG-nitroarginine methyl ester (L-NAME), which is non-selective NO-synthase inhibitor, at a dose of 25 mg/kg for 7 days. The second group (the control group) got 0.9% sodium chloride solution administered as intraperito

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9

Stout, M. A., J. S. Saenz, G. T. Gentry, S. P. Leibo, K. R. Bondioli, and R. A. Godke. "168 POST-THAW VIABILITY OF EPIDIDYMAL SPERM FROM WHITE-TAIL DEER THAT WAS FROZEN USING GLYCEROL OR DMSO AS THE CRYOPROTECTANT." Reproduction, Fertility and Development 21, no. 1 (2009): 183. http://dx.doi.org/10.1071/rdv21n1ab168.

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Cryoprotectants are compounds that protect and maintain the viability of cells when being subjected to cold environments. In addition to glycerol, DMSO has been evaluated for post-thaw epididymal sperm viability in various endangered species, but has not yet been assessed in White-tail deer. Testicles (in the scrotum) were collected from hunter harvested sexually mature White-tail bucks (n = 7) during the peak rutting season. Testicles within the scrotum, once removed from the postmortem buck, were placed in a plastic Ziploc bag and then into a Styrofoam ice chest (at ambient temperature) and

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Olukole, Samuel Gbadebo, and Bankole Olusiji Oke. "Morphology of the Testis and Epididymis of Large White Boars." Turkish Journal of Agriculture - Food Science and Technology 4, no. 5 (2016): 374. http://dx.doi.org/10.24925/turjaf.v4i5.374-377.634.

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The testis and epididymis of twenty five adult Large White boars were used to investigate the biometric and histomorphometric parameters of the testis and epididymis of the boars. The aim of the study was to provide information which could be useful in the comparative regional anatomy of the male reproductive organs of domestic animals and thus an improved assessment of breeding soundness and fertility potential in boars. The average weight of the animals was 71.3 ± 10.7 kg. The average weights of the right and left testes were 170 ± 0.7.60 g and 179±6.48g, respectively with no significant dif

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Thimmaiah, Vishwanath T. "ADENOMATOID TUMOR OF TAIL OF EPIDIDYMIS - A CASE REPORT." Journal of Evolution of Medical and Dental sciences 2, no. 32 (2013): 6061–65. http://dx.doi.org/10.14260/jemds/1099.

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Brown, D., B. Lui, S. Gluck, and I. Sabolic. "A plasma membrane proton ATPase in specialized cells of rat epididymis." American Journal of Physiology-Cell Physiology 263, no. 4 (1992): C913—C916. http://dx.doi.org/10.1152/ajpcell.1992.263.4.c913.

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Acidification of the luminal fluid in the epididymis is believed to play an important role in sperm maturation. Previous studies have shown that specialized cells in the epithelium lining the epididymis contain high levels of carbonic anhydrase and that these cells have rod-shaped intramembraneous particles when examined by freeze fracture. Both of these features are characteristic of proton-transporting intercalated cells in the kidney collecting duct. We now show that apical cells in the head of the epididymis and clear cells in the body and tail of the epididymis express high levels of a va

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Krishnan, Sharath K., Ravindran Chirukandath, Safna Kunhikandilakath, Harikumar Kaniyamparambil Vava, and Babu Pulluvelil John. "Epidydimal leiomyosarcoma: a rare case of scrotal swelling." International Surgery Journal 7, no. 7 (2020): 2429. http://dx.doi.org/10.18203/2349-2902.isj20202864.

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Sarcomas of the genitourinary tract are extremely uncommon and accounts for only 1-2% of genito urinary malignancies. Sarcomas of the para testicular region, comprising tissues such as the epididymis, spermatic cord, inguinal canal and testicular tunica are also extremely rare. epidydimal leiomyosarcoma accounted only for 4 percentage of all para testicular tumours and only 16 cases are reported so far in literature and they account 4% of all Para testicular sarcomas. We are presenting a 61-year-old patient presented with a hard welling of 1 year duration, with no other associated symptoms. On

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Hadley, J. A., J. C. Hall, A. O'Brien, and R. Ball. "Effects of a simulated microgravity model on cell structure and function in rat testis and epididymis." Journal of Applied Physiology 72, no. 2 (1992): 748–59. http://dx.doi.org/10.1152/jappl.1992.72.2.748.

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A tail-suspension (TS) rat model used to simulate microgravity was tested for its effects on the anatomy, cell structure, and function of the testis and epididymis in sexually mature male rats. Rats suspended for 7 days without inguinal canal ligation exhibited a significant (P less than or equal to 0.05) reduction in testis weight compared with controls (1.55 +/- 0.04 to 1.1 +/- 0.02 g). Except for the liver, epididymis, and adrenals of TS rats and TS rats allowed to recover for 7 days, no significant (P less than or equal to 0.05) change was observed in the weight of other body and accessory

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Zvyagina, V. I., and E. S. Belskikh. "Carnitine chloride reduces the severity of experimental hyperhomocysteinemia and promotes lactate utilization by mitochondrial fraction of the rat epididymis." Biomeditsinskaya Khimiya 67, no. 4 (2021): 338–46. http://dx.doi.org/10.18097/pbmc20216704338.

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Hyperhomocysteinemia is a risk factor for many diseases, including reproductive disorders in men. L-carnitine is used in medical practice to correct impaired bioenergetic conditions; in patients with idiopathic forms of infertility its effects are associated with improvement of the sperm parameters. However, the effect of exogenous L-carnitine on the level of homocysteine in the gonadal tissues, as a risk factor for impaired fertility, has not been investigated yet. The aim of this study was to investigate activity of bioenergetic enzymes in the epididymal mitochondrial fraction, the dynamics

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Zayed, Ahmed El-Zuhry, Khaled Hamdy Aly, Ismael Abdel-Aziz Ibrahim, and Fatma Mohammed Abd El-maksoud. "Morphological studies on the seasonal changes in the epididymal duct of the one-humped camel (camelus dromedarius)." Veterinary Science Development 2, no. 1 (2012): 3. http://dx.doi.org/10.4081/vsd.2012.3997.

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The present work was carried out on 20 testes and epididymis of sexually mature camels to elucidate the gross anatomical, morphometerical, light microscopical and scanning electron microscopical features of the epididymis in different seasons. Anatomically, the epididymal duct of a camel consists of three parts head, body and tail. Histomorphologically, the epididymal duct is subdivided into initial, middle and terminal segments, of which the middle segment is further subdivided into proximal, intermediate and distal parts. There is a gradual decrease in the epithelial height of the epididymal

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Vishwanath, TT, Simran Singh, SMCC Shetty, and MK Ajay. "Adenomatoid Tumour of the Epididymis: A Case Report." Nepalese Journal of Radiology 3, no. 1 (2013): 80–83. http://dx.doi.org/10.3126/njr.v3i1.8817.

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Adenomatoid tumours are rare benign neoplasms involving the paratesticular regions, most commonly the tail of epididymis, however they involve various other structures like genitourinary system. Here, we report a case of adenomatoid tumor of the epididymis its ultrasound imaging features and review of literature. Nepalese Journal of Radiology / Vol.3 / No.1 / Issue 4 / Jan-June, 2013 / 80-83 DOI: http://dx.doi.org/10.3126/njr.v3i1.8817

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Zhang, Xiaoning, Wenwen Zhou, Peng Zhang, et al. "Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm." International Journal of Molecular Sciences 22, no. 2 (2021): 652. http://dx.doi.org/10.3390/ijms22020652.

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The calcium-binding protein spermatid-associated 1 (Cabs1) is a novel spermatid-specific protein. However, its function remains largely unknown. In this study, we found that a long noncoding RNA (lncRNA) transcripted from the Cabs1 gene antisense, AntiCabs1, was also exclusively expressed in spermatids. Cabs1 and AntiCabs1 knockout mice were generated separately (using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 methods) to investigate their functions in spermatogenesis. The genetic loss of Cabs1 did not affect testicular and epididymal development; however, male mic

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Zhang, Xiaoning, Wenwen Zhou, Peng Zhang, et al. "Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm." International Journal of Molecular Sciences 22, no. 2 (2021): 652. http://dx.doi.org/10.3390/ijms22020652.

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The calcium-binding protein spermatid-associated 1 (Cabs1) is a novel spermatid-specific protein. However, its function remains largely unknown. In this study, we found that a long noncoding RNA (lncRNA) transcripted from the Cabs1 gene antisense, AntiCabs1, was also exclusively expressed in spermatids. Cabs1 and AntiCabs1 knockout mice were generated separately (using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 methods) to investigate their functions in spermatogenesis. The genetic loss of Cabs1 did not affect testicular and epididymal development; however, male mic

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Roy, Jeremy W., Eric Hill, Ye Chun Ruan, et al. "Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis." American Journal of Physiology-Cell Physiology 305, no. 4 (2013): C436—C446. http://dx.doi.org/10.1152/ajpcell.00410.2012.

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Clear cells express the vacuolar proton-pumping H+-ATPase (V-ATPase) and acidify the lumen of the epididymis, a process that is essential for male fertility. The renin-angiotensin-aldosterone system (RAAS) regulates fluid and electrolyte balance in the epididymis, and a previous study showed binding of aldosterone exclusively to epididymal clear cells (Hinton BT, Keefer DA. Steroid Biochem 23: 231–233, 1985). We examined here the role of aldosterone in the regulation of V-ATPase in the epididymis. RT-PCR showed expression of the mineralocorticoid receptor [MR; nuclear receptor subfamily 3, gro

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Pandey, Shivesh, Suresh Vasant Phatak, Gopidi Sai Nidhi Reddy, and Apoorvi Bharat Shah. "Post Traumatic Hematocele - Ultrasonography and Elastography Imaging." Journal of Evolution of Medical and Dental Sciences 10, no. 21 (2021): 1636–38. http://dx.doi.org/10.14260/jemds/2021/340.

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Hematocele with blunt scrotal trauma is an uncommon cause of the testicular pain. Elastography is the new recent advance in the field of ultrasound. USG and elastography findings of the acute hematocele is described in this aricle. Testicular trauma is the third most common cause of acute scrotal pain,1 and high-frequency ultrasonography (USG) with a linear array transducer is the first preferred modality for testicular trauma evaluation. Extra testicular haematoceles or blood collections inside the tunica vaginalis are the most common findings in the scrotum after blunt injury.2 On clinical a

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Perez, E. G. A., M. Nichi, C. A. Baptista Sobrinho, et al. "240 FUNCTIONAL TRAITS OF CAT SPERM DURING DISTINCT MATURATION STATUS." Reproduction, Fertility and Development 23, no. 1 (2011): 218. http://dx.doi.org/10.1071/rdv23n1ab240.

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Sperm recovery from the caudae epididymides can be advantageous for preserving semen of endangered animal species. In this context, the domestic cat is a suitable model for the study of sperm physiology in endangered feline species and the research on epididymal sperm preservation combined with the use of reproductive biotechnologies including intracytoplasmic sperm injection (ICSI). The aim of the present study was to examine the sperm collected from the cauda and caput of the cat epididymis using functional tests. Testicles and epididymides from 5 adult tomcats were collected by orchiectomy

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B. "Production of Male-Sterility Mouse by Resection of the Tail Epididymis." American Journal of Animal and Veterinary Sciences 7, no. 2 (2012): 75–79. http://dx.doi.org/10.3844/ajavsp.2012.75.79.

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Manske, Marianne, Beate Welte, Karen Hitschke, Andreas Wack, and Ernesto G. Bade. "Plasminogen activatior inhibitor type 1 in rat spermatozoa: localisation in the tail, in the acrosome and on the surface of the head." Zygote 2, no. 3 (1994): 243–52. http://dx.doi.org/10.1017/s0967199400002045.

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SummaryIsolated seminiferous tubules of rat testis contain considerable urokinase-inhibiting activity. An immunohistological analysis revealed the presence of plasminogen activator inhibitor type 1 (PAI-1) in the basement membrane as well as in the interior of the tubules. Distribution and intensity of the intratubular immunoreactivity depends on the tubules. Distribution and intensity of the intratubular immunoreactivity depends on the stage of the seminiferous cycle. A relatively weak signal is present around elongated nuclei of spermatids at the beginning of chromatin condensation. The sign

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Olukunle, J. O., E. B. Jacobs, J. A. Oyewusi, L. A. Durotoye, and E. O. Adeleye. "Effects of aqueous leaf extract of Tithonia diversifolia (Mexican Sunflower) on semen characteristic and morphology in male Wistar albino rats." Nigerian Journal of Animal Production 42, no. 2 (2015): 263–70. http://dx.doi.org/10.51791/njap.v42i2.2684.

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The objective of the present study was to investigate the effects of the aqueous extract of Tithonia diversifolia leaves on semen characteristics and spermatozoa morphology Wistar rats. The rats were divided into three groups (A, B and C) and treated daily with 400, 800 and 1600 mg/kg of the aqueous extract of Tithonia diversifolia leaves respectively for 28 days while 5ml/kg of distilled water was given to the control group D. At the end of the experimental period, animals were sacrificed and sperm characteristics, histology of the testes and epididymis were assessed. Aqueous extract of Titho

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Ciszewska-Piłczyńska, A., and A. Barańczyk-Kuźma. "Glutathione conjugation in male reproductive system: studies on glutathione-S-transferase of bull and boar epididymis." Acta Biochimica Polonica 47, no. 1 (2000): 223–31. http://dx.doi.org/10.18388/abp.2000_4081.

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Male reproductive organs are extremely sensitive to the negative influence of toxic environmental factors as well as drugs, and until now not many attempts have been made at studying the detoxication enzymes and the relationship between the activity of those enzymes and spermatozoa fertility. In the present work we studied cytosolic glutathione-S-transferases (GST, EC 2.5.1.18) from different parts (head, corpus and tail) of bull and boar epididymis. We isolated two molecular forms of GST from each part of epididymis, characterized their biochemical properties and examined the mechanism of the

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Fernandez-Fuertes, B., F. Narciandi, K. G. Meade, C. O'Farrelly, S. Fair та P. Lonergan. "3 ROLE OF β-DEFENSIN 126 IN PROMOTING SPERM MOTILITY IN CATTLE". Reproduction, Fertility and Development 28, № 2 (2016): 131. http://dx.doi.org/10.1071/rdv28n2ab3.

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As immature sperm migrate through the epididymis, they are bathed in region-specific epididymal fluid, which leads to a sequential addition, deletion, and modification of their surface proteins. These changes ultimately result in the acquisition of motility and fertilising abilities. Among the hundreds of proteins secreted by the epididymis, several β-defensins have been identified and correlated with male fertility in multiple species. In cattle, β-defensin 126 (BD126) is exclusively detected in the reproductive tract of pubertal males, with preferential mRNA expression in the epididymis. Bot

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Kuzmina, N. V., D. D. Ostapiv, O. I. Chajkovska, R. D. Ostapiv, and O. P. Panych. "ACTIVITY OF MALATE-ASPARTATE SHUTTLE ENZYMES OF BULLS AND BOARS REPRODUCTIVE ORGANS AND EPIDIDIMAL SPERMATOZOA." Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology 21, no. 2 (2020): 86–89. http://dx.doi.org/10.36359/scivp.2020-21-2.11.

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The activity of malate-aspartate shuttle enzymes in the reproductive organs and epididimal sperm of bulls and boars was studied. The research was conducted on bulls of the Ukrainian black-spotted dairy breed (n = 5; aged 14 - 16 months) and boars of the Great White breed (n = 3; aged 10 - 12 months). After slaughter, the testes and testicular appendages were removed and the spermatozoa were washed with 0.9% sodium chloride solution. Testicular and epididimal tissues were homogenized and centrifuged. Aspartate aminotransferase (AST), malate dehydrogenase (MDG) and protein concentration we

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Corrêa, Lanna B. N. S., Ludmila F. M. de F. Cardozo, Ilma C. de A. Ribeiro, Gilson T. Boaventura, and Maurício A. Chagas. "Influence of prolonged flaxseed ( Linum usitatissimum ) consumption over epididymis and testicle histoarchitecture of Wistar rats." Pesquisa Veterinária Brasileira 37, no. 6 (2017): 650–56. http://dx.doi.org/10.1590/s0100-736x2017000600020.

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ABSTRACT: Flaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG),

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Nazar, AL-Khafaji. "The effect of Ricinus communis seeds extract on reproductive activity and blood values of male rabbits." Al-Anbar Journal of Veterinary Sciences 12, no. 1 (2019): 97–106. http://dx.doi.org/10.37940/ajvs.2019.12.1.11.

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Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treate

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Andraszek, Katarzyna, Dorota Banaszewska, Małgorzata Szostek, Ewa Wójcik, Magdalena Czubaszek, and Renata Walczak-Jędrzejowska. "Use of Silver Nitrate for the Assessment of Sperm Measurements in Selected Farm and Free-Living Animal Species." Bulletin of the Veterinary Institute in Pulawy 58, no. 3 (2014): 487–94. http://dx.doi.org/10.2478/bvip-2014-0074.

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Abstract The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with silver nitrate - AgNO3. The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO3 staining differentiated the acrosoma

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Putranti, Oktora Dwi. "Pengaruh Kafein Terhadap Lengkung Ekor Spermatozoa Epididimis Sapi Bali." AGRISAINTIFIKA: Jurnal Ilmu-Ilmu Pertanian 2, no. 2 (2019): 143. http://dx.doi.org/10.32585/ags.v2i2.265.

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This study aims to analyze the influence of caffeine on the distance of the tail movement of the spermatozoa epididymis cattle Bali. Methods in this study with a collection of sperm from Slaughter House (RPH) and then conducted the process of freezing with Tris yolk dilution. Treatment of caffeine with levels of 0 mg/ml, 2 mg/ml, 4 mg/ml and 6 mg/ml in spermatozoa that have been in thawing. The variables observed were DCL, DAP, DSL, ALH analyzed by CASA. Results showed no difference (P> 0.05) motility with caffeine treatment and short sperm tailwave distance of 37.27 μm showed the sperm mot

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Moore, Alison, Kathy M. Ensrud, Thomas W. White, Chris D. Frethem, and David W. Hamilton. "Rat epididymis-specific sperm maturation antigens. I. Evidence that the 26 kD 4E9 antigen found on rat caudal epididymal sperm tail is derived from a protein secreted by the epididymis." Molecular Reproduction and Development 37, no. 2 (1994): 181–94. http://dx.doi.org/10.1002/mrd.1080370209.

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LIN, MINJIE, and JOHN C. RODGER. "Acrosome formation during sperm transit through the epididymis in two marsupials, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula)." Journal of Anatomy 194, no. 2 (1999): 223–32. http://dx.doi.org/10.1017/s0021878299004586.

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In certain Australian marsupials including the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula), formation of the acrosome is not completed in the testis but during a complex differentiation process as spermatozoa pass through the epididymis. Using transmission and scanning electron microscopy this paper defined the process of acrosome formation in the epididymis, providing temporal and spatial information on the striking reorganisation of the acrosomal membranes and matrix and of the overlying sperm surface involved. On leaving the testis wallaby and possum s

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Tanaka, Atsushi. "A new sperm collection method for in-vitro fertilization: collection at tail of epididymis." Human Reproduction 7, no. 6 (1992): 838–40. http://dx.doi.org/10.1093/oxfordjournals.humrep.a137746.

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Goyal, Hari O., Carol S. Williams, Mohammed K. Khalil, Madan M. Vig, and M. A. Maloney. "Postnatal differentiation of the ductus deferens, tail of the epididymis, and distal body of the epididymis in goats occurs independently of rete testis fluid." Anatomical Record 254, no. 4 (1999): 508–20. http://dx.doi.org/10.1002/(sici)1097-0185(19990401)254:4<508::aid-ar6>3.0.co;2-a.

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Pera, Ilka, Petra Derr, Ching-Hei Yeung, Trevor G. Cooper, and Christiane Kirchhoff. "Regionalized expression of CD52 in rat epididymis is related to mRNA poly(A) tail length." Molecular Reproduction and Development 48, no. 4 (1997): 433–41. http://dx.doi.org/10.1002/(sici)1098-2795(199712)48:4<433::aid-mrd3>3.0.co;2-r.

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Oko, R., L. Hermo, P. T. Chan, A. Fazel, and J. J. Bergeron. "The cytoplasmic droplet of rat epididymal spermatozoa contains saccular elements with Golgi characteristics." Journal of Cell Biology 123, no. 4 (1993): 809–21. http://dx.doi.org/10.1083/jcb.123.4.809.

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The cytoplasmic droplet of epididymal spermatozoa is a small localized outpouching of cytoplasm of the tail of unknown significance. EM revealed flattened saccular elements as the near exclusive membranous component of the droplet. Light and electron microscopic immunolabeling for Golgi/TGN markers showed these saccules to be reactive for antibodies to TGN38, protein affinity-purified alpha 2,6 sialyltransferase, and anti-human beta 1,4 galactosyltransferase. The saccules were isolated by subcellular fractionation and antibodies raised against this fraction immunolabeled the saccules of the dr

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Nishimura, R. C., I. R. Bessa, M. A. N. Dode, and M. M. Franco. "241 METHYLATION PROFILE OF XIST GENE EXON 1 IN BOVINE SPERMATOGENIC CELLS." Reproduction, Fertility and Development 25, no. 1 (2013): 268. http://dx.doi.org/10.1071/rdv25n1ab241.

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Mammalian embryos undergo two waves of genome reprogramming, the first in the zygote, when paternal and maternal genomes are demethylated and, shortly thereafter, remethylated, and the second in the primordial germ cells. It is believed that remethylation is reestablished in male germ cells before birth; however, it is not clear when and how this process occurs in the bovine. Thus, this work aimed to verify whether epigenetic reprogramming occurs during spermatogenesis by evaluating the methylation profile of XIST gene exon 1 in different spermatogenic cells. Spermatocytes, elongated spermatid

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Li, Ruilan, Xiaomei Fan, Tong Zhang, et al. "Expression of selenium-independent glutathione peroxidase 5 (GPx5) in the epididymis of Small Tail Han sheep." Asian-Australasian Journal of Animal Sciences 31, no. 10 (2018): 1591–97. http://dx.doi.org/10.5713/ajas.18.0015.

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Fàbrega, Anna, Marta Puigmulé, Jean-Louis Dacheux, Sergi Bonet, and Elisabeth Pinart. "Glycocalyx characterisation and glycoprotein expression of Sus domesticus epididymal sperm surface samples." Reproduction, Fertility and Development 24, no. 4 (2012): 619. http://dx.doi.org/10.1071/rd11064.

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The sperm surface is covered with a dense coating of carbohydrate-rich molecules. Many of these molecules are involved in the acquisition of fertilising ability. In the present study, eight lectins (i.e. Arachis hypogae (peanut) agglutinin (PNA), Lens culimaris (lentil) agglutinin-A (LCA), Pisum sativum (pea) agglutin (PSA), Triticum vulgari (wheat) germ agglutinin (WGA), Helix pomatia agglutinin (HPA), Phaseolus vulgaris (red kidney bean) leucoagglutinin (PHA-L), Glycine max (soybean) agglutinin (SBA) and Ulex europaeus agglutinin I (UEA-I)) were investigated to identify changes in the nature

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Koppers, A. J., G. M. Gibbs, T. Reddy, P. McIntyre, and M. K. O'Bryan. "182. ROLE OF CRISP4 IN ION CHANNEL REGULATION AND MALE REPRODUCTION." Reproduction, Fertility and Development 22, no. 9 (2010): 100. http://dx.doi.org/10.1071/srb10abs182.

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CRISPs are a group of 3 proteins found in mammals (4 in the mouse) which show a strong expression bias in the male reproductive organs. Whilst the function of most CRISPs are yet to be elucidated, mouse CRISP2 is a known regulator of the ion channel, ryanodine receptor. CRISP4 is most abundantly produced by the principal cells of the epididymis and are secreted into the lumen, where they adhere to sperm during epididymal transit. In this study we examined the role of CRISP4 ion channel regulation in mouse spermatozoa through cell assays and mouse models. The identification of the Transient Rec

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Sienkiewicz, Waldemar, Aleksander Szczurkowski, Agnieszka Dudek, and Jerzy Kaleczyc. "Innervation of the chinchilla testis, epididymis, and vas deferens." Bulletin of the Veterinary Institute in Pulawy 59, no. 4 (2015): 547–56. http://dx.doi.org/10.1515/bvip-2015-0082.

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Abstract The study was performed on six male chinchillas. The animals were anaesthetised with ether and the anaesthesia was deepened with nembuthal injected intraperitoneally. The chinchillas were then transcardially perfused with 0.4 L of 4% buffered paraformaldehyde, and testes, epididymides, and vasa deferentia were collected. The tunica albuginea from one testis from each chinchilla was stained as whole-mount preparation. The tissues were cut into 12 μm-thick cryostat sections, and processed for double-immunofluorescence method. In all organs studied, the most abundant nerve fibres were do

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Hourcade, J. D., M. Perez-Crespo, B. Pintado, and A. Gutiérrez-Adán. "3 SELECTION OF UNFRAGMENTED-DNA SPERMATOZOA FROM HEAT STRESSED MICE BY FEMALE UTERINE TRACT AND ZONA PELUCIDA BINDING." Reproduction, Fertility and Development 21, no. 1 (2009): 102. http://dx.doi.org/10.1071/rdv21n1ab3.

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Physiological bases of the sperm selection processes within the female reproductive tract before they meet and fertilize the oocyte are unknown. The aim of this work was to determine if one of the keys of spermatozoa selection could be DNA integrity. It has been reported that sperm DNA damage does not impair in vitro fertilization (IVF). However, it has been suggested that the zona pelucida (ZP) is able to select spermatozoa with unfragmented DNA (Liu and Baker 2007 Hum. Reprod. 22, 1597–1602). In this work, DNA damage of spermatozoa was artificially induced by scrotal heat treatment (HT) (42°

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Andraszek, Katarzyna, Dorota Banaszewska, Olga Szeleszczuk, Piotr Niedbała, and Marta Kuchta‐Gładysz. "Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis." Reproduction in Domestic Animals 55, no. 4 (2020): 515–22. http://dx.doi.org/10.1111/rda.13646.

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Guo, F., B. Yang, Z. H. Ju, et al. "Alternative splicing, promoter methylation, and functional SNPs of sperm flagella 2 gene in testis and mature spermatozoa of Holstein bulls." REPRODUCTION 147, no. 2 (2014): 241–52. http://dx.doi.org/10.1530/rep-13-0343.

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The sperm flagella 2 (SPEF2) gene is essential for development of normal sperm tail and male fertility. In this study, we characterized first the splice variants, promoter and its methylation, and functional single-nucleotide polymorphisms (SNPs) of theSPEF2gene in newborn and adult Holstein bulls. Four splice variants were identified in the testes, epididymis, sperm, heart, spleen, lungs, kidneys, and liver tissues through RT-PCR, clone sequencing, and western blot analysis. Immunohistochemistry revealed that theSPEF2was specifically expressed in the primary spermatocytes, elongated spermatid

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Redivo Júnior, Nildo, Camila Dutra de Souza, Ellyn Amanda Fonseca Martins, et al. "Scrotal thermography to assess the seminal quality in Nelore and Girolando bulls." Semina: Ciências Agrárias 41, no. 1 (2020): 131. http://dx.doi.org/10.5433/1679-0359.2020v41n1p131.

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Bull selection by andrological examination aims to estimate the reproductive capacity of the male. Bulls of zebu origin adapt better to high temperatures than bulls of taurine origin, which may influence scrotum temperature and seminal quality due to the imbalance of testicular thermoregulation. The objective of this study was to investigate the relationship between bioclimatic variables, the temperature of body and scrotal areas, assessed with infrared thermography, and the quality of fresh and post-thawed semen in zebu Nelore bulls (Bos taurus indicus) and Girolando bulls (Bos taurus taurus

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Okada, Fatima Kazue, Rhayza Roberta Andretta, and Deborah Montagnini Spaine. "One day is better than four days of ejaculatory abstinence for sperm function." Reproduction and Fertility 1, no. 1 (2020): 1–10. http://dx.doi.org/10.1530/raf-20-0018.

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According to the World Health Organization guidelines, ejaculatory abstinence (EA) of 2–7 days is recommended for semen analysis. This study aimed to determine how seminal quality may be affected by two EA periods from the same man. Seminal samples from 65 men were evaluated by conventional semen analysis and qualitative characteristics after 1 and 4 days of EA (two samples/man). The semen was qualitatively analyzed by examining oxidative activity (intracellular and seminal plasma), sperm function (acrosome integrity, mitochondrial activity, and nuclear DNA integrity), and epididymal function.

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Souza, Anne K., Luiz Guilherme C. Trautwein, Cristiane S. Paranzini, et al. "Effect of refrigeration at -1°C on spermatozoa quality of domestic cats." Pesquisa Veterinária Brasileira 40, no. 4 (2020): 306–14. http://dx.doi.org/10.1590/1678-5150-pvb-6517.

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ABSTRACT: The objective of this study was to evaluate the sperm quality obtained of domestic cats by electroejaculation and recovery of the tail of the epididymis after cooling at -1°C and 4°C for 24 and 48 hours. Twenty-nine adult cats (2 to 6kg) were used. Sperm collection was performed by electroejaculation (EEJ), and after 48 hours, the cats were orchiectomized, and sperm sample was obtained from the vas deferens and epididymis tail (EPD). The samples were diluted in ACP-117® extender, and the sperm characteristics were evaluated at three different moments: when still fresh, 24 and 48 hour

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Xie, Minyu, Guofei Zhang, Hanbin Zhang, et al. "Adenylate kinase 1 deficiency disrupts mouse sperm motility under conditions of energy stress†." Biology of Reproduction 103, no. 5 (2020): 1121–31. http://dx.doi.org/10.1093/biolre/ioaa134.

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Abstract Mammalian spermatozoa are highly polarized cells characterized by compartmentalized cellular structures and energy metabolism. Adenylate kinase (AK), which interconverts two ADP molecules into stoichiometric amounts of ATP and AMP, plays a critical role in buffering adenine nucleotides throughout the tail to support flagellar motility. Yet the role of the major AK isoform, AK1, is still not well characterized. Here, by using a proteomic analysis of testis biopsy samples, we found that AK1 levels were significantly decreased in nonobstructive azoospermia patients. This result was furth

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