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Journal articles on the topic 'Epithelial cells – Electric properties'

Author: Grafiati

Published: 4 June 2021

Last updated: 19 February 2022

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1

Shckorbatov, Yury G., Valery G. Shakhbazov, and Andrey O. Rudenko. "Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields." Bioelectromagnetics 22, no. 2 (2001): 106–11. http://dx.doi.org/10.1002/1521-186x(200102)22:2<106::aid-bem1013>3.0.co;2-2.

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Nevala, H., T. Ylikomi, and H. Tähti. "Evaluation of the selected barrier properties of retinal pigment epithelial cell line ARPE-19 for an in-vitro blood-brain barrier model." Human & Experimental Toxicology 27, no. 10 (2008): 741–49. http://dx.doi.org/10.1177/0960327107082230.

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In-vitro models that maintain complex transport mechanisms and structural properties associated with the blood-brain barrier in vivo would be useful in drug permeability and neurotoxicological studies. To evaluate the suitability of a human retinal pigment epithelial cell line for a blood-brain barrier model, we have compared the barrier properties of the human retinal pigment epithelial cell line ARPE-19, the human colonic adenocarcinoma cell line Caco-2, and primary porcine microvessel endothelial cells. The tight junction proteins occludin and ZO-1 were stained immunocytochemically. The par

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Cramer, E. B., L. C. Milks, M. J. Brontoli, G. K. Ojakian, S. D. Wright, and H. J. Showell. "Effect of human serum and some of its components on neutrophil adherence and migration across an epithelium." Journal of Cell Biology 102, no. 5 (1986): 1868–77. http://dx.doi.org/10.1083/jcb.102.5.1868.

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The effect of human serum and some of its components on the process of transepithelial migration of human neutrophils was investigated in an in vitro system. 10% autologous serum caused an increase in neutrophil adherence to and migration across canine kidney epithelial cells. This increase in neutrophil binding also occurred if the epithelium but not the neutrophils had been preincubated with serum. The binding was lost if the serum was either preabsorbed over the kidney epithelium before use or heat inactivated. Indirect immunofluorescence studies indicated that IgG, IgM, and a component of

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4

Oshima, Tadayuki, Karin Gedda, Junichi Koseki, et al. "Establishment of esophageal-like non-keratinized stratified epithelium using normal human bronchial epithelial cells." American Journal of Physiology-Cell Physiology 300, no. 6 (2011): C1422—C1429. http://dx.doi.org/10.1152/ajpcell.00376.2010.

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Current experimental models of esophageal epithelium in vitro suffer from either poor differentiation or complicated culture systems. We have established a model to study stratified squamous epithelium in vitro, which is very similar to esophageal epithelium in vivo. A stratified squamous multilayer epithelium was formed by seeding primary normal human bronchial epithelial (NHBE) cells onto collagen- and fibronectin-coated trans-well inserts and then cultivating the cells under air-liquid interface (ALI) conditions in the presence of growth factors and low levels of all-trans-retinoic acid. Tr

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5

Becker, Ulrich, Carsten Ehrhardt, Marc Schneider, et al. "A Comparative Evaluation of Corneal Epithelial Cell Cultures for Assessing Ocular Permeability." Alternatives to Laboratory Animals 36, no. 1 (2008): 33–44. http://dx.doi.org/10.1177/026119290803600106.

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The purpose of this study was to evaluate the potential value of different epithelial cell culture systems as in vitro models for studying corneal permeability. Transformed human corneal epithelial (HCE-T) cells and Statens Serum Institut rabbit corneal (SIRC) cells were cultured on permeable filters. SkinEthic human corneal epithelium (S-HCE) and Clonetics human corneal epithelium (C-HCE) were received as ready-to-use systems. Excised rabbit corneas (ERCs) and human corneas (EHCs) were mounted in Ussing chambers, and used as references. Barrier properties were assessed by measuring transepith

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6

Leung, Clarus, Samuel J. Wadsworth, S. Jasemine Yang, and Delbert R. Dorscheid. "Structural and functional variations in human bronchial epithelial cells cultured in air-liquid interface using different growth media." American Journal of Physiology-Lung Cellular and Molecular Physiology 318, no. 5 (2020): L1063—L1073. http://dx.doi.org/10.1152/ajplung.00190.2019.

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The human bronchial epithelium is an important barrier tissue that is damaged or pathologically altered in various acute and chronic respiratory conditions. To represent the epithelial component of respiratory disease, it is essential to use a physiologically relevant model of this tissue. The human bronchial epithelium is a highly organized tissue consisting of a number of specialized cell types. Primary human bronchial epithelial cells (HBEC) can be differentiated into a mucociliated tissue in air-liquid interface (ALI) cultures using appropriately supplemented media under optimized growth c

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Lavelle, J. P., H. O. Negrete, P. A. Poland, et al. "Low permeabilities of MDCK cell monolayers: a model barrier epithelium." American Journal of Physiology-Renal Physiology 273, no. 1 (1997): F67—F75. http://dx.doi.org/10.1152/ajprenal.1997.273.1.f67.

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Barrier epithelia such as the renal collecting duct (in the absence of antidiuretic hormone) and thick ascending limb, as well as the stomach and mammalian bladder, exhibit extremely low permeabilities to water and small nonelectrolytes. A cell culture model of such epithelia is needed to determine how the structure of barrier apical membranes reduce permeability and how such membranes may be generated and maintained. In the present studies, the transepithelial electrical resistance and isotopic water and urea fluxes were measured for Madin-Darby canine kidney (MDCK) type I and type II cells,

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Lazarus, S. C., L. J. McCabe, J. A. Nadel, W. M. Gold, and G. D. Leikauf. "Effects of mast cell-derived mediators on epithelial cells in canine trachea." American Journal of Physiology-Cell Physiology 251, no. 3 (1986): C387—C394. http://dx.doi.org/10.1152/ajpcell.1986.251.3.c387.

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We examined the interaction between mast cell-derived mediators and the electrical and ion transport properties of canine tracheal epithelium. We compared the effect of mediators released by immunologic challenge of sensitized lung parenchyma with that of mediators released from canine mastocytoma cells challenged with calcium ionophore A23187. Short-circuit current (Isc) increased by 19.2 +/- 3.0 microA/cm2 in response to mediators released from sensitized lung fragments challenged with ragweed antigen. This effect was not due to histamine. When the epithelial tissues were pretreated with ind

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9

Jiang, T., and A. Holley. "Some properties of receptive fields of olfactory mitral/tufted cells in the frog." Journal of Neurophysiology 68, no. 3 (1992): 726–33. http://dx.doi.org/10.1152/jn.1992.68.3.726.

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1. Different regions of the frog's olfactory epithelium were stimulated with nine glass micropipettes either individually or simultaneously in different combinations. The stimulus was a positive electrical pulse (4 s) consisting of a progressive increase, a plateau, and a progressive decrease in current intensity. Extra- and intracellular recordings were made from olfactory bulb mitral/tufted cells. Some of these cells were identified by intracellular injection of Lucifer yellow. 2. The action potential response patterns of mitral/tufted cells during the different phases of the stimulation wer

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10

Gróf, Ilona, Alexandra Bocsik, András Harazin, et al. "The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel." International Journal of Molecular Sciences 21, no. 11 (2020): 4024. http://dx.doi.org/10.3390/ijms21114024.

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Clinical and experimental results with inhaled sodium bicarbonate as an adjuvant therapy in cystic fibrosis (CF) are promising due to its mucolytic and bacteriostatic properties, but its direct effect has not been studied on respiratory epithelial cells. Our aim was to establish and characterize co-culture models of human CF bronchial epithelial (CFBE) cell lines expressing a wild-type (WT) or mutant (deltaF508) CF transmembrane conductance regulator (CFTR) channel with human vascular endothelial cells and investigate the effects of bicarbonate. Vascular endothelial cells induced better barrie

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11

Kong, Steffi Shi Qing, Alejandra Martinez, and Maddy Behravan. "Investigation of Normal Cell and Cancer Cell Attachment and the Effects of Ganoderma Lucidum Using an Electric Impedance Sensing Technique." MRS Advances 5, no. 45 (2020): 2341–48. http://dx.doi.org/10.1557/adv.2020.313.

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AbstractThis research introduces an application of an electric impedance sensing technique to investigate cell attachment of normal epithelial cells (HaCAT) and cancerous cells (A431) before and after addition of Ganoderma Lucidum (reishi). In this study, an impedance sensing system is used to measure and characterize real-time changes in electric impedance (resistance and capacitance) with respect to an alternating current (AC) applied to HaCAT and A431 cell colonies. The impedance data is related to the properties of cell spreading, attachment, and delamination. The effect of reishi at dosag

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Bekusova, Viktoria, Linda Droessler, Salah Amasheh, and Alexander G. Markov. "Effects of 1,2-Dimethylhydrazine on Barrier Properties of Rat Large Intestine and IPEC-J2 Cells." International Journal of Molecular Sciences 22, no. 19 (2021): 10278. http://dx.doi.org/10.3390/ijms221910278.

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Colon cancer is accompanied by a decrease of epithelial barrier properties, which are determined by tight junction (TJ) proteins between adjacent epithelial cells. The aim of the current study was to analyze the expression of TJ proteins in a rat model of 1,2-dimethylhydrazine (DMH)-induced colorectal cancer, as well as the barrier properties and TJ protein expression of IPEC-J2 cell monolayers after incubation with DMH. Transepithelial electrical resistance and paracellular permeability for sodium fluorescein of IPEC-J2 were examined by an epithelial volt/ohm meter and spectrophotometry. The

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Ricciardi, Mario, Martina Midolo, Giulio Bassi, et al. "Comparison Between Bone Marrow Mesenchymal Stromal Cells (BM-MSC) and Lung Mesenchymal Stromal Cells (Lung-MSC) For Epithelial Regeneration." Blood 122, no. 21 (2013): 5414. http://dx.doi.org/10.1182/blood.v122.21.5414.5414.

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Abstract Developing a therapeutic strategy for lung regeneration still remains complex. Stem cell-based therapeutical approaches have been suggested as a potential tool; among them, human mesenchymal stromal cells (MSC) possess some promising features to this aim. MSC are stem cells residing in many tissue, i.e. bone marrow (BM), adipose tissue, cord blood, lung, etc., and they are capable of differentiating into different cell types of mesodermal origin, such as fat, bone and cartilage. To assess MSC epithelial differentiation potential, through a partially known process called Mesenchymal to

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14

Sapich, Sandra, Marius Hittinger, Remi Hendrix-Jastrzebski, et al. "Murine Alveolar Epithelial Cells and Their Lentivirus-mediated Immortalisation." Alternatives to Laboratory Animals 46, no. 2 (2018): 73–89. http://dx.doi.org/10.1177/026119291804600207.

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In this study, we describe the isolation and immortalisation of primary murine alveolar epithelial cells (mAEpC), as well as their epithelial differentiation and barrier properties when grown on Transwell® inserts. Like human alveolar epithelial cells (hAEpC), mAEpC transdifferentiate in vitro from an alveolar type II (ATII) phenotype to an ATI-like phenotype and exhibit features of the air–blood barrier, such as the establishment of a thin monolayer with functional tight junctions (TJs). This is demonstrated by the expression of TJ proteins (ZO-1 and occludin) and the development of high tran

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Jentsch, T. J., H. Matthes, S. K. Keller, and M. Wiederholt. "Electrical properties of sodium bicarbonate symport in kidney epithelial cells (BSC-1)." American Journal of Physiology-Renal Physiology 251, no. 6 (1986): F954—F968. http://dx.doi.org/10.1152/ajprenal.1986.251.6.f954.

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Intracellular potentials of BSC-1 kidney epithelial cells known to express a Na+-HCO3- symport ranged between -40 and -70 mV, mean value Vm = -55.1 +/- 10.1 mV. Lowering HCO3- at constant partial pressure of CO2 (PCO2) or complete removal of HCO3-/CO2, rapidly depolarized, whereas readding HCO3- hyperpolarized Vm (40 +/- 8 mV/decade HCO3- at constant PCO2). This response was largely reduced by 1 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) (which depolarized Vm to about -20 mV) and in Na+-free medium, but Ba2+ had no effect. In HCO3(-)-Ringer, Na+ removal rapidly depolarized Vm (

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16

Grasset, E., J. Bernabeu, and M. Pinto. "Epithelial properties of human colonic carcinoma cell line Caco-2: effect of secretagogues." American Journal of Physiology-Cell Physiology 248, no. 5 (1985): C410—C418. http://dx.doi.org/10.1152/ajpcell.1985.248.5.c410.

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Human colonic carcinoma Caco-2 cells grown in vitro form epithelial layers of highly polarized cells. Unlike colonic adsorptive cells they possess a mucosal membrane with very limited ionic conductance, even after exposure to aldosterone. When grown on filters, Caco-2 cells were sensitive to various secretagogues; these included 10(-5) M dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and 10(-10) M vasoactive intestinal peptide, both of which, added serosally, enhanced the short-circuit current. The same applied to mucosal forskolin. Caco-2 cell sensitivity to serosal epinephrine was l

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17

Ben Lagha, Amel, Patricia Maquera Huacho, and Daniel Grenier. "A cocoa (Theobroma cacao L.) extract impairs the growth, virulence properties, and inflammatory potential of Fusobacterium nucleatum and improves oral epithelial barrier function." PLOS ONE 16, no. 5 (2021): e0252029. http://dx.doi.org/10.1371/journal.pone.0252029.

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Fusobacterium nucleatum is associated with many conditions and diseases, including periodontal diseases that affect tooth-supporting tissues. The aim of the present study was to investigate the effects of a cocoa extract (Theobroma cacao L.) on F. nucleatum with respect to growth, biofilm formation, adherence, and hydrogen sulfide (H2S) production. The anti-inflammatory properties and the effect on epithelial barrier function of the cocoa extract were also assessed. The cocoa extract, whose major phenolic compound is epicatechin, dose-dependently inhibited the growth, biofilm formation, adhere

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18

Tamò, Luca, Youssef Hibaoui, Sampada Kallol, et al. "Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells." American Journal of Physiology-Lung Cellular and Molecular Physiology 315, no. 6 (2018): L921—L932. http://dx.doi.org/10.1152/ajplung.00357.2017.

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Differentiation of primary alveolar type II epithelial cells (AEC II) to AEC type I in culture is a major barrier in the study of the alveolar epithelium in vitro. The establishment of an AEC II cell line derived from induced pluripotent stem cells (iPSC) represents a novel opportunity to study alveolar epithelial cell biology, for instance, in the context of lung injury, fibrosis, and repair. In the present study, we generated long-lasting AEC II from iPSC (LL-iPSC-AEC II). LL-iPSC-AEC II displayed morphological characteristics of AEC II, including growth in a cobblestone monolayer, the prese

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19

Bhattacharjee, Promita, and Mark Ahearne. "Fabrication and Biocompatibility of Electroconductive Silk Fibroin/PEDOT: PSS Composites for Corneal Epithelial Regeneration." Polymers 12, no. 12 (2020): 3028. http://dx.doi.org/10.3390/polym12123028.

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The aim of this study was to develop matrices that can support human corneal epithelial cells and innervation by incorporating a conducting polymer, poly(3,4-ethylenedioxythiophene) poly(styrene sulfonate) (PEDOT:PSS), into silk fibroin (SF). Polyvinyl alcohol (PVA) was used as a crosslinking agent to enhance the mechanical properties of the matrices. The impact of PEDOT:PSS on the materials’ physical properties and cellular responses was examined. The electrical impedance of matrices decreased with increasing concentration of PEDOT:PSS suggesting improved electroconductivity. However, light t

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Mathias, R. T., and J. L. Rae. "Transport properties of the lens." American Journal of Physiology-Cell Physiology 249, no. 3 (1985): C181—C190. http://dx.doi.org/10.1152/ajpcell.1985.249.3.c181.

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Many studies have shown that the lens is a multicellular syncytial tissue whose electrophysiological properties are the integrated result of membrane transport, low-resistance gap junctions interconnecting the cells, and the restricted extracellular space between cells. There are at least three structurally distinct populations of cells within the lens, and the membrane transport properties of each cell type appear to differ. Indeed, there may be subcellular specialization of membrane transport properties in the surface epithelial cells. We review the physical structure of the lens, its electr

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Donaldson, P. J., Y. Dong, M. Roos, C. Green, D. A. Goodenough, and J. Kistler. "Changes in lens connexin expression lead to increased gap junctional voltage dependence and conductance." American Journal of Physiology-Cell Physiology 269, no. 3 (1995): C590—C600. http://dx.doi.org/10.1152/ajpcell.1995.269.3.c590.

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The differentiation of mouse lens epithelial cells into fiber cells is a useful model for studying the changes of the electrical properties of gap junction (cell-to-cell) channels that are induced by an alteration in connexin expression patterns. In this model, cuboidal lens epithelial cells differentiate into elongated fiber cells, and the expression of connexin43 (Cx43) in the epithelial cells is replaced with the production of high levels of Cx50 and Cx46 in the fiber cells. We now report a new procedure to isolate mouse lens fiber cell pairs suitable for double whole cell patch-clamp analy

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Metz, Julia Katharina, Birgit Wiegand, Sabrina Schnur, et al. "Modulating the Barrier Function of Human Alveolar Epithelial (hAELVi) Cell Monolayers as a Model of Inflammation." Alternatives to Laboratory Animals 48, no. 5-6 (2020): 252–67. http://dx.doi.org/10.1177/0261192920983015.

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The incidence of inflammatory lung diseases such as acute respiratory distress syndrome (ARDS) remains an important problem, particularly in the present time with the Covid-19 pandemic. However, an adequate in vitro test system to monitor the barrier function of the alveolar epithelium during inflammation and for assessing anti-inflammatory drugs is urgently needed. Therefore, we treated human Alveolar Epithelial Lentivirus-immortalised cells (hAELVi cells) with the pro-inflammatory cytokines TNF-α (25 ng/ml) and IFN-γ (30 ng/ml), in the presence or absence of hydrocortisone (HC). While TNF-α

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Cotton, C. U., and L. al-Nakkash. "Isolation and culture of bovine pancreatic duct epithelial cells." American Journal of Physiology-Gastrointestinal and Liver Physiology 272, no. 6 (1997): G1328—G1337. http://dx.doi.org/10.1152/ajpgi.1997.272.6.g1328.

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We describe a method to isolate and culture epithelial cells from the main duct of the bovine pancreas. In primary cultures, secretin caused a dose-dependent increase in intracellular adenosine 3',5'-cyclic monophosphate (cAMP) and stimulated electrogenic transepithelial ion transport. Elevation of intracellular cAMP increased the rate coefficient for 36Cl- efflux from 0.14 +/- 0.03 to 0.47 +/- 0.12 min-1, and plasma membrane conductance, measured by the whole cell patchclamp technique, was increased from 0.7 +/- 0.1 to 6.9 +/- 0.8 nS. The cAMP-activated anion currents had properties similar t

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Guerra, Michael H., Thangal Yumnamcha, Abdul-Shukkur Ebrahim, Elizabeth A. Berger, Lalit Pukhrambam Singh, and Ahmed S. Ibrahim. "Real-Time Monitoring the Effect of Cytopathic Hypoxia on Retinal Pigment Epithelial Barrier Functionality Using Electric Cell-Substrate Impedance Sensing (ECIS) Biosensor Technology." International Journal of Molecular Sciences 22, no. 9 (2021): 4568. http://dx.doi.org/10.3390/ijms22094568.

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Disruption of retinal pigment epithelial (RPE barrier integrity is a hallmark feature of various retinal blinding diseases, including diabetic macular edema and age-related macular degeneration, but the underlying causes and pathophysiology are not completely well-defined. One of the most conserved phenomena in biology is the progressive decline in mitochondrial function with aging leading to cytopathic hypoxia, where cells are unable to use oxygen for energy production. Therefore, this study aimed to thoroughly investigate the role of cytopathic hypoxia in compromising the barrier functionali

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Awayda, Mouhamed S., Abderrahmane Bengrine, Nelia A. Tobey, James D. Stockand, and Roy C. Orlando. "Nonselective cation transport in native esophageal epithelia." American Journal of Physiology-Cell Physiology 287, no. 2 (2004): C395—C402. http://dx.doi.org/10.1152/ajpcell.00412.2003.

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Rabbit esophageal epithelia actively transport Na+ in a manner similar to that observed in classic electrically tight Na+-absorbing epithelia, such as frog skin. However, the nature of the apical entry step is poorly understood. To address this issue, we examined the electrophysiological and biochemical nature of this channel. Western blotting experiments with epithelial Na+ channel (ENaC) subunit-specific antibodies revealed the presence of all three ENaC subunits in both native and immortalized esophageal epithelial cells. The amino acid sequence of the rabbit α-ENaC cloned from native rabbi

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Brookes, Oliver, Sonja Boland, René Lai Kuen, Dorian Miremont, Jamileh Movassat, and Armelle Baeza-Squiban. "Co-culture of type I and type II pneumocytes as a model of alveolar epithelium." PLOS ONE 16, no. 9 (2021): e0248798. http://dx.doi.org/10.1371/journal.pone.0248798.

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The epithelial tissues of the distal lung are continuously exposed to inhaled air, and are of research interest in studying respiratory exposure to both hazardous and therapeutic materials. Pharmaco-toxicological research depends on the development of sophisticated models of the alveolar epithelium, which better represent the different cell types present in the native lung and interactions between them. We developed an air-liquid interface (ALI) model of the alveolar epithelium which incorporates cell lines which bear features of type I (hAELVi) and type II (NCI-H441) epithelial cells. We comp

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Woodall, M., J. Jacob, K. K. Kalsi, et al. "E-cigarette constituents propylene glycol and vegetable glycerin decrease glucose uptake and its metabolism in airway epithelial cells in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 319, no. 6 (2020): L957—L967. http://dx.doi.org/10.1152/ajplung.00123.2020.

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Electronic nicotine delivery systems, or e-cigarettes, utilize a liquid solution that normally contains propylene glycol (PG) and vegetable glycerin (VG) to generate vapor and act as a carrier for nicotine and flavorings. Evidence indicated these “carriers” reduced growth and survival of epithelial cells including those of the airway. We hypothesized that 3% PG or PG mixed with VG (3% PG/VG, 55:45) inhibited glucose uptake in human airway epithelial cells as a first step to reducing airway cell survival. Exposure of H441 or human bronchiolar epithelial cells (HBECs) to PG and PG/VG (30–60 min)

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Nilsson, Mikael, Johanna Husmark, Bengt Nilsson, Lars-Erik Tisell, and Lars E. Ericson. "Primary culture of human thyrocytes in Transwell bicameral chamber: thyrotropin promotes polarization and epithelial barrier function." European Journal of Endocrinology 135, no. 4 (1996): 469–80. http://dx.doi.org/10.1530/eje.0.1350469.

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Nilsson M, Husmark J, Nilsson B, Tisell L-E, Ericson LE. Primary culture of human thyrocytes in Transwell bicameral chamber: thyrotropin promotes polarization and epithelial barrier function. Eur J Endocrinol 1996;135:469–80. ISSN 0804–4643 Epithelial properties of thyrocytes are difficult to maintain in conventional cell culture systems. We used bicameral chambers (Transwell™) in attempts to establish a functional epithelium of thyrocytes of human origin. Thyroid follicle segments were isolated by collagenase digestion of paradenomatous tissue obtained at surgery for follicular adenoma and of

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Yeung, Chun-Yan, Jen-Shiu Chiang Chiau, Wai-Tao Chan, et al. "In VitroPrevention ofSalmonellaLipopolysaccharide-Induced Damages in Epithelial Barrier Function by VariousLactobacillusStrains." Gastroenterology Research and Practice 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/973209.

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Background.Lactobacillusshows beneficial anti-inflammatory effects onSalmonellainfection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. WhetherLactobacilluscould be used to regulate the TJ protein expression and distribution in inflamed intestinal epithelial cells was determined.Methods. Using the transwell coculture model,Salmonellalipopolysaccharide (LPS) was apically added to polarized Caco-2 cells cocultured with peripheral blood mononuclear cells in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with

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Li, Fenfang, Igor Cima, Jess Honganh Vo, Min-Han Tan, and Claus Dieter Ohl. "Single Cell Hydrodynamic Stretching and Microsieve Filtration Reveal Genetic, Phenotypic and Treatment-Related Links to Cellular Deformability." Micromachines 11, no. 5 (2020): 486. http://dx.doi.org/10.3390/mi11050486.

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Deformability is shown to correlate with the invasiveness and metastasis of cancer cells. Recent studies suggest epithelial-to-mesenchymal transition (EMT) might enable cancer metastasis. However, the correlation of EMT with cancer cell deformability has not been well elucidated. Cellular deformability could also help evaluate the drug response of cancer cells. Here, we combine hydrodynamic stretching and microsieve filtration to study cellular deformability in several cellular models. Hydrodynamic stretching uses extensional flow to rapidly quantify cellular deformability and size with high t

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Rabinovich, Y., M. Esayanur, S. Daosukho, K. Byer, H. El-Shall, and S. Khan. "Atomic force microscopy measurement of the elastic properties of the kidney epithelial cells." Journal of Colloid and Interface Science 285, no. 1 (2005): 125–35. http://dx.doi.org/10.1016/j.jcis.2004.11.041.

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Jovov, B., N. K. Wills, and S. A. Lewis. "A spectroscopic method for assessing confluence of epithelial cell cultures." American Journal of Physiology-Cell Physiology 261, no. 6 (1991): C1196—C1203. http://dx.doi.org/10.1152/ajpcell.1991.261.6.c1196.

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We describe a convenient nonelectrophysiological technique for assessing cell proliferation and subsequent tight junction formation for epithelial monolayers grown on permeable supports. The method involves the use of phenol red (PR), a standard pH indicator in most cell culture media. In addition, we report a systematic error in a commercially available system for measuring transepithelial electrical properties. Briefly, the flux of PR across the epithelium was measured from the serosal solution into the mucosal solution. The mucosal solution was first replaced with a PR-free solution and the

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Clark, Farmehini, Spiers, Woolf, Swami, and Landers. "Real Time Electronic Feedback for Improved Acoustic Trapping of Micron-Scale Particles." Micromachines 10, no. 7 (2019): 489. http://dx.doi.org/10.3390/mi10070489.

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Acoustic differential extraction has been previously reported as a viable alternative to the repetitive manual pipetting and centrifugation steps for isolating sperm cells from female epithelial cells in sexual assault sample evidence. However, the efficiency of sperm cell isolation can be compromised in samples containing an extremely large number of epithelial cells. When highly concentrated samples are lysed, changes to the physicochemical nature of the medium surrounding the cells impacts the acoustic frequency needed for optimal trapping. Previous work has demonstrated successful, automat

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Chinet, T. C., J. M. Fullton, J. R. Yankaskas, R. C. Boucher, and M. J. Stutts. "Sodium-permeable channels in the apical membrane of human nasal epithelial cells." American Journal of Physiology-Cell Physiology 265, no. 4 (1993): C1050—C1060. http://dx.doi.org/10.1152/ajpcell.1993.265.4.c1050.

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We used patch-clamp techniques to study the channels that underlie the Na+ conductance of the apical membrane of human normal nasal epithelial cells. Cells were cultured on permeable supports and studied after confluence. In 172 of 334 (52%) excised membrane patches, we observed 20-pS Na(+)-permeable channels that do not discriminate between Na+ and K+ (pNa/pK = 1.33). These nonselective cation channels contained subpopulations that differed by dependence of open probability on voltage and bath Ca2+ activity, suggesting two or more channel types with similar electrical properties. In the prese

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Wohlwend, A., J. D. Vassalli, D. Belin, and L. Orci. "LLC-PK1 cells: cloning of phenotypically stable subpopulations." American Journal of Physiology-Cell Physiology 250, no. 5 (1986): C682—C687. http://dx.doi.org/10.1152/ajpcell.1986.250.5.c682.

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The LLC-PK1 pig kidney-derived cell line is morphologically and functionally heterogeneous. We have clonally derived three sublines that differ in their response to calcitonin and in their ability to form domes. The three clones were analyzed for their basal and hormonally induced plasminogen activator production. In contrast to the D + Sc clone, in which calcitonin induced a greater than 100-fold increase in plasminogen activator synthesis, the D + Rc clone did not respond to the hormone; this was related to a deficiency of the cells in calcitonin binding. Transepithelial electrical resistanc

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Schlegel, Nicolas, Michael Meir, Wolfgang-Moritz Heupel, Bastian Holthöfer, Rudolf E. Leube, and Jens Waschke. "Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." American Journal of Physiology-Gastrointestinal and Liver Physiology 298, no. 5 (2010): G774—G783. http://dx.doi.org/10.1152/ajpgi.00239.2009.

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The integrity of intercellular junctions that form the “terminal bar” in intestinal epithelium is crucial for sealing the intestinal barrier. Whereas specific roles of tight and adherens junctions are well known, the contribution of desmosomal adhesion for maintaining the intestinal epithelial barrier has not been specifically addressed. For the present study, we generated a desmoglein 2 antibody directed against the extracellular domain (Dsg2 ED) to test whether impaired Dsg2-mediated adhesion affects intestinal epithelial barrier functions in vitro. This antibody was able to specifically blo

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Furuse, Mikio, Kyoko Furuse, Hiroyuki Sasaki, and Shoichiro Tsukita. "Conversion of Zonulae Occludentes from Tight to Leaky Strand Type by Introducing Claudin-2 into Madin-Darby Canine Kidney I Cells." Journal of Cell Biology 153, no. 2 (2001): 263–72. http://dx.doi.org/10.1083/jcb.153.2.263.

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There are two strains of MDCK cells, MDCK I and II. MDCK I cells show much higher transepithelial electric resistance (TER) than MDCK II cells, although they bear similar numbers of tight junction (TJ) strands. We examined the expression pattern of claudins, the major components of TJ strands, in these cells: claudin-1 and -4 were expressed both in MDCK I and II cells, whereas the expression of claudin-2 was restricted to MDCK II cells. The dog claudin-2 cDNA was then introduced into MDCK I cells to mimic the claudin expression pattern of MDCK II cells. Interestingly, the TER values of MDCK I

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Benedé, Sara, Ana Gradillas, Mayte Villalba, and Eva Batanero. "Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function." Nutrients 11, no. 6 (2019): 1303. http://dx.doi.org/10.3390/nu11061303.

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Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles

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Han, Chaoqun, Zhen Ding, Huiying Shi, Wei Qian, Xiaohua Hou, and Rong Lin. "The Role of Probiotics in Lipopolysaccharide-Induced Autophagy in Intestinal Epithelial Cells." Cellular Physiology and Biochemistry 38, no. 6 (2016): 2464–78. http://dx.doi.org/10.1159/000445597.

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Background/Aims: Dysfunction of autophagy has been associated with loss of intestinal homeostasis. Lipopolysaccharide (LPS) from Gram-negative bacteria is known to be a major initiator of intestinal epithelial cell (IEC) autophagy. Although probiotics have been recognized to be involved in many therapeutic properties and participate in host defense responses, the molecular mechanisms by which probiotics exert these positive effects remain unknown. This study assessed the effect of probiotics on LPS-induced physical barrier dysfunction and the underlying mechanism of probiotic action in IECs wi

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Ferrell, Nicholas, Joseph Groszek, Lingyan Li, et al. "Basal lamina secreted by MDCK cells has size- and charge-selective properties." American Journal of Physiology-Renal Physiology 300, no. 1 (2011): F86—F90. http://dx.doi.org/10.1152/ajprenal.00484.2010.

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The role electrical charge plays in determining glomerular permeability to macromolecules remains unclear. If the glomerular basement membrane (GBM) has any significant role in permselectivity, physical principles would suggest a negatively charged GBM would reject similarly charged more than neutral species. However, recent in vivo studies with negative and neutral glomerular probes showed the opposite. Whether this observation is due to unique characteristics of the probes used or is a general physiological phenomenon remains to be seen. The goal of this study was to use the basement membran

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Barker, P. M., R. C. Boucher, and J. R. Yankaskas. "Bioelectric properties of cultured monolayers from epithelium of distal human fetal lung." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 2 (1995): L270—L277. http://dx.doi.org/10.1152/ajplung.1995.268.2.l270.

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Throughout intrauterine life, Cl(-)-rich liquid is secreted by the pulmonary epithelium. To evaluate the role of the most distal epithelium in liquid secretion, we measured bioelectric properties of monolayers composed of epithelial cells from acinar structures of postmortem human fetal lung (mean gestation, 22.3 wk; range, 18-24 wk). These monolayers formed high-resistance (R) barriers (mean R = 363 Ohm/cm2) when cultured in hormone-supplemented, serum-free medium. The transepithelial electrical potential difference (4.0 mV, lumen negative), was similar to that of whole fetal sheep lung in vi

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Seeber, Judith W., Michaela Zorn-Kruppa, Simone Lombardi-Borgia, et al. "Characterisation of Human Corneal Epithelial Cell Cultures Maintained under Serum-free Conditions." Alternatives to Laboratory Animals 36, no. 5 (2008): 569–83. http://dx.doi.org/10.1177/026119290803600512.

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Three-dimensional tissue constructs have been proposed as in vitro screening models for ocular irritancy. Based on our previous studies, in which a full-thickness corneal model based exclusively on SV40-immortalised cell lines was generated, we have currently evaluated the effects of a range of commercially-available cell culture media on several cellular parameters in cultures of a human corneal epithelial (HCE) cell line. This cell line was used in an attempt to establish a rational basis for the development of serum-free culture media for the assembly and long-term tissue culture of full-th

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Di Cristo, Bianchi, Chiu, et al. "Comparative in Vitro Cytotoxicity of Realistic Doses of Benchmark Multi-Walled Carbon Nanotubes towards Macrophages and Airway Epithelial Cells." Nanomaterials 9, no. 7 (2019): 982. http://dx.doi.org/10.3390/nano9070982.

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Multi-walled carbon nanotubes (MWCNT) have many outstanding physical and chemical properties that make them useful in many applications in nanotechnology. However, these properties are reported to be potentially harmful for the human body. The effects of low and realistic doses of three well-characterized preparations of MWCNT, obtained from the Joint Research Centre (JRC) (NM-400, NM-401, and NM-402), were assessed in two murine macrophage lines, Raw264.7, of peritoneal origin, and MH-S, derived from alveolar macrophages. Macrophage viability, evaluated with two distinct methods, was signific

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Leung, Lawrence W., Ruben G. Contreras, Catalina Flores-Maldonado, Marcelino Cereijido, and Enrique Rodriguez-Boulan. "Inhibitors of glycosphingolipid biosynthesis reduce transepithelial electrical resistance in MDCK I and FRT cells." American Journal of Physiology-Cell Physiology 284, no. 4 (2003): C1021—C1030. http://dx.doi.org/10.1152/ajpcell.00149.2002.

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Madin-Darby canine kidney (MDCK) I and Fisher rat thyroid (FRT) cells exhibit transepithelial electrical resistance (TER) values in excess of 5,000 Ω · cm2. When these cells were incubated in the presence of various inhibitors of sphingolipid biosynthesis, a >5-fold reduction of TER was observed without changes in the gate function for uncharged solutes or the fence function for apically applied fluorescent lipids. The localization of ZO-1 and occludin was not altered between control and inhibitor-treated cells, indicating that the tight junction was still intact. Furthermore, the complexit

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Hebert, Steven C., Gary Desir, Gerhard Giebisch, and Wenhui Wang. "Molecular Diversity and Regulation of Renal Potassium Channels." Physiological Reviews 85, no. 1 (2005): 319–71. http://dx.doi.org/10.1152/physrev.00051.2003.

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K+ channels are widely distributed in both plant and animal cells where they serve many distinct functions. K+ channels set the membrane potential, generate electrical signals in excitable cells, and regulate cell volume and cell movement. In renal tubule epithelial cells, K+ channels are not only involved in basic functions such as the generation of the cell-negative potential and the control of cell volume, but also play a uniquely important role in K+ secretion. Moreover, K+ channels participate in the regulation of vascular tone in the glomerular circulation, and they are involved in the m

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Mitic, Laura L., Christina M. Van Itallie, and James M. Anderson. "Molecular Physiology and Pathophysiology of Tight Junctions I. Tight junction structure and function: lessons from mutant animals and proteins." American Journal of Physiology-Gastrointestinal and Liver Physiology 279, no. 2 (2000): G250—G254. http://dx.doi.org/10.1152/ajpgi.2000.279.2.g250.

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Tight junctions form the major paracellular barrier in epithelial tissues. Barrier-sealing properties are quite variable among cell types in terms of electrical resistance, solute and water flux, and charge selectivity. A molecular explanation for this variability appears closer following identification of the transmembrane proteins occludin and members of the claudin multigene family. For example, the human phenotype of mutations in claudin-16 suggests that it creates a channel that allows magnesium to diffuse through renal tight junctions. Similarly, a mouse knockout of claudin-11 reveals it

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Veronesi, Bellina, Kent Carlsón, and Marion Ehrich. "An In Vitro Model of the Blood-Brain Barrier: The Response of Madin-Darby Canine Kidney Cells to Triethyl Tin." Alternatives to Laboratory Animals 24, no. 3 (1996): 349–57. http://dx.doi.org/10.1177/026119299602400308.

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The development of a cell culture model which simulates the properties of the blood–brain barrier (BBB) is necessary for the detection of neurotoxic chemicals that can disrupt the barrier, and to provide a more “risk relevant” in vitro screening battery. The present study evaluates the Madin-Darby canine kidney (MDCK) epithelial cell line for this purpose. Changes in electrical resistance and enzyme activities were correlated in confluent MDCK cells exposed to the neurotoxic metal, triethyl tin (TET). Concentrations of TET (0.001–10μM) were established that produced depression in electrical re

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Van Itallie, Christina M., Alan S. Fanning, and James M. Anderson. "Reversal of charge selectivity in cation or anion-selective epithelial lines by expression of different claudins." American Journal of Physiology-Renal Physiology 285, no. 6 (2003): F1078—F1084. http://dx.doi.org/10.1152/ajprenal.00116.2003.

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Tight junctions (TJ) regulate paracellular ionic charge selectivity and conductance across epithelial tissues and cell lines. These properties vary among epithelia, and recent evidence implicates the claudins, a family of TJ transmembrane proteins, as important determinants of both characteristics. To test the hypothesis that each claudin contributes a characteristic charge discrimination to the TJ, we expressed claudins-2, -4, -11, and -15 in both cation-selective Madin-Darby canine kidney (MDCK) II cells and in anion-selective LLC-PK1 cells and examined changes in transepithelial electrical

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Grumbach, Yael, Nga Vu Thi Quynh, Raphaël Chiron, and Valérie Urbach. "LXA4 stimulates ZO-1 expression and transepithelial electrical resistance in human airway epithelial (16HBE14o-) cells." American Journal of Physiology-Lung Cellular and Molecular Physiology 296, no. 1 (2009): L101—L108. http://dx.doi.org/10.1152/ajplung.00018.2008.

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Lipoxin A4 (LXA4) is a biologically active eicosanoid produced in human airways that displays anti-inflammatory properties. In cystic fibrosis and severe asthma, LXA4 production has been reported to be decreased, and, in such diseases, one of the consequences of airway inflammation is disruption of the tight junctions. In the present study, we investigated the possible role of LXA4 on tight junction formation, using transepithelial electrical resistance (TER) measurements, Western blotting, and immunofluorescence. We observed that exposure to LXA4 (100 nM) for 2 days significantly increased zo

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Walsh-Reitz, Margaret M., Erick F. Huang, Mark W. Musch, et al. "AMP-18 protects barrier function of colonic epithelial cells: role of tight junction proteins." American Journal of Physiology-Gastrointestinal and Liver Physiology 289, no. 1 (2005): G163—G171. http://dx.doi.org/10.1152/ajpgi.00013.2005.

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Antrum mucosal protein (AMP)-18 and a synthetic peptide of amino acids 77–97 have mitogenic and motogenic properties for epithelial cells. The possibility that AMP-18 is also protective was evaluated in the colonic mucosa of mice and monolayer cultures of human colonic epithelial Caco-2/bbe (C2) cells. Administration of AMP peptide to mice with dextran sulfate sodium (DSS)-induced colonic injury delayed the onset of bloody diarrhea and reduced weight loss. Treatment of C2 cells with AMP peptide protected monolayers against decreases in transepithelial electrical resistance induced by the oxida

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