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1
Maile, Charlotte Amy. "Pathophysiology of equine type1 polysaccharide storage myopathy." Thesis, Royal Veterinary College (University of London), 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.618291.
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Orme, Elizabeth Catherine. "Fat metabolism in the exercising thoroughbred horse." Thesis, Open University, 1995. http://oro.open.ac.uk/57558/.
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The thoroughbred horse has been selectively bred for speed and has a high capacity for carbohydrate metabolism. The following series of studies investigated the relative contribution of fat and carbohydrate to energy production during exercise of varying intensity. Furthermore the work assessed the capacity of the horse to increase the contribution of fat to energy production as the result of either an acute increase in the availability of plasma free fatty acids (FFA) or as the result of chronic fat supplementation. Finally an adaptational response to feeding a fat supplemented diet was described. The variation in plasma long chain FFA over a 24 hour period was described. The early hours of the morning represented the period of greatest variability in plasma FFA concentration. This period was characterised by a significant increase in total and individual FFA concentration, which was unrelated to feed intake. As a result of the reported circadian rhythm in plasma FFA all subsequent exercise studies were performed during the period of least variability in plasma FFA concentration. A model for the pre-exercise elevation of plasma FFA, using a combination of a triglyceride emulsion and the heparinoid type substance pentosan polysulphate, was used to investigate the effect of increased FFA availability on fat utilisation during prolonged low intensity exercise. Pentosan polysulphate was used in preference to heparin following an investigation of their relative lipolytic and anticoagulative properties. Pentosan polysulphate when administered at 3 times the dose of heparin resulted in a comparable increase in plasma total lipase activity. When co-administered with a triglyceride emulsion, pentosan polysulphate resulted in a similar increase in plasma FFA concentration relative to that produced with the same triglyceride emulsion and heparin. The anticoagulative effect of pentosan polysulphate, however, was approximately 9 times less than that of heparin, as measured by activated partial thromboplastin time. The contribution of fat and carbohydrate to energy production during exercise was influenced by both the intensity and duration of exercise, as indicated by measurements of respiratory exchange ratio (RER). The inter-horse variability in RER was greatest during low intensity exercise. An increase in the contribution of carbohydrate to energy production occurred at the onset and during the early stages of prolonged exercise and as the result of an increase in exercise intensity. A proportion of horses exhibited an increase in the utilisation of fat during low intensity prolonged exercise as a result of a pre-exercise elevation in plasma FFA concentration. RER was consistently lower during exercise in 5 out of the 7 horses studied following a pre-exercise elevation of plasma FFA. Furthermore, plasma glucose concentration was elevated above that observed during the control session in 4 of these 5 horses for at least the first 15 minutes of exercise. A prolonged period of fat supplementation resulted in an improved management of the fat load. Following 10 weeks of dietary treatment a significant increase in plasma cholesterol concentration and a significant decrease in plasma triglyceride concentration was reported. The decrease in plasma triglyceride concentration was associated with a mean 50% increase in post pentosan polysulphate plasma total lipase activity. It is suggested that the increase in the post pentosan polysulphate plasma total lipase activity may have reflected an increase in muscle lipoprotein lipase activity. A significant increase in the activity of muscle citrate synthase was observed during the period of fat supplementation. No significant change occurred in muscle ß-hydroxyacyl CoA dehydrogenase activity or in the concentration of resting muscle glycogen and triglyceride as a result of fat supplementation. RER was significantly lower in the latter stages of prolonged low intensity exercise, during the period of fat supplementation, relative to the same exercise performed before the introduction and following 5 weeks of withdrawal of the fat supplemented diet. The reduction in RER during the period of fat supplementation was associated with a greater exercise induced increase in plasma FFA concentration. The above differences were also apparent during moderate intensity exercise, although, examination of the individual horse data revealed that the effect was not as clear as that observed during low intensity exercise. No significant differences were reported in either RER or plasma FFA concentration in response to moderate/high intensity exercise during the period of fat supplementation. Neither were any significant differences observed in either RER or plasma FFA concentration in the control group at any exercise intensity. An increased availability of plasma FFA and an increase in the oxidative capacity of muscle, as well as an enhanced ability to utilise plasma triglycerides may have contributed to the increase in fat utilisation, observed during low and moderate intensity exercise, in response to fat supplementation. The effect of differences in the hormonal response to a fat supplemented diet as a precipitant of the observed adaptational responses in these studies requires further investigation.
3
Richards, Claire Myfanwy. "The production and evaluation of equine/murine heterohybridomas that secrete monoclonal antibodies to influenza A equine 2 virus." Thesis, Open University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262653.
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Littlefield, Joanne. "Improved Horse Breeding: Equine Reproduction Research and Education." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2005. http://hdl.handle.net/10150/622196.
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5
Williams, Catherine Felicia. "African horse sickness virus genes and gene products." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312217.
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Harris, Patricia Ann. "Aspects of the equine rhabdomyolysis syndrome." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257296.
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Ellis, Andrea Dorothea. "Ingestive and digestive processes in the horse." Thesis, University of Essex, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252270.
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Brüning, Anke. "Serodiagnosis of equine babesiosis by ELISA." Thesis, Imperial College London, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306891.
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Zhang, John J. "In vitro studies on equine gametes." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239777.
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10
Mair, T. S. "Respiratory immunology and hypersensitivity in the horse." Thesis, University of Bristol, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370896.
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11
Monteiro, Susana Oliveira Serrano. "Distal limb osteoarthritis in the horse." Doctoral thesis, Universidade de Évora, 2016. http://hdl.handle.net/10174/18560.
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The aim of this thesis was to study two objective methods of osteoarthritis (OA) diagnosis in
horses and use them on the assessment of new intra-articular treatments. The studied methods were
a new inertial-sensor based system of lameness detection and cartilage biomarkers in serum. It was
found that distal limb flexion is significantly correlated to the presence of metacarpo-phalangeal OA in
hind limbs and that inertial-sensors are sensitive in detecting asymmetry in these cases. A positive
and significant correlation was observed between Coll2-1 concentration in serum and the presence of
joint disease in males and young horses. Fib3-2 measurement has good potential to be used since it
is not influenced by sex or age. Using an experimental model of OA, adipose stem cells pre-activated
with interferon-gamma decreased joint inflammation and radiographic lesions. In clinical cases, a
single injection of high-concentrated and high-molecular weight hyaluronic-acid decreased joint
inflammation and biomarkers’ concentration; OSTEOARTRITE DO MEMBRO DISTAL NO CAVALO
Resumo:
A finalidade desta tese foi estudar dois métodos de diagnóstico objetivo de osteoartrite (OA) em
equinos e aplicá-los na avaliação de novas terapias intra-articulares. Utilizou-se um sistema de
sensores de movimento e foi avaliada a concentração de biomarcadores de cartilagem no soro.
Concluiu-se que a flexão distal positiva está correlacionada com OA na articulação metacarpofalângica
nos membros posteriores e que os sensores são sensíveis na detecção de assimetria
nestes casos. Existe uma correlação positiva e significativa entre as concentrações de Coll2-1 e a
presença de doença articular, sobretudo em machos e jovens. A dosagem de Fib3-2 tem utilidade
por não ser influenciada pelo sexo nem idade. Num modelo experimental da doença, a terapia à
base de células estaminais reduziu a inflamação articular e as lesões radiográficas. Em casos
clínicos, o tratamento com ácido-hialurónico de alta concentração e peso molecular provoca uma
diminuição da inflamação articular e dos biomarcadores no soro.
12
Griffiths, Nicola Jane. "Studies on Clostridium perfringens in the horse." Thesis, University of Liverpool, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367091.
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Seabury, Ashley Gustafson. "Physical mapping and characterization of the equine lymphocyte antigen (ELA) complex." Texas A&M University, 2005. http://hdl.handle.net/1969.1/2345.
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The major histocompatibility complex (MHC) is a genomic region comprised of a linked
cluster of genes and gene families that play an important role in both the adaptive and
innate immune responses. Genes within the MHC have also been associated with
susceptibility and/or resistance to certain diseases, such as haemochromatosis, insulindependent
diabetes, and psoriasis. As a result of these associations the MHC is one the
most extensively studied regions of the mammalian genome. The MHCs of a wide
variety of species, such as human (HLA), mouse (H-2), pig (SLA), and cow (BoLA),
have been characterized with respect to gene content, genomic organization of class I,
class II, and class III regions, and comparative organization. Comparative analyses have
been useful in delineating the evolutionary development of the MHC.
While the MHC of many mammalian species has been investigated, little research has
been performed on the equine (Equus caballus) MHC. The equine MHC is referred to
as the equine lymphocyte antigen (ELA) complex and is located on chromosome
ECA20q. The research that has been done on ELA focused on identifying gene copy
number and genetic polymorphisms in the classical class I and class II genes. To better
characterize the gene content and organization of ELA, we isolated 103 bacterial
artificial chromosome (BAC) clones from a horse BAC library containing well
conserved genes found within mammalian MHCs. These BAC clones were assembled
into two sequence-ready ordered contigs that span the ELA complex. The first contig
which has a minimum tiling path of nine BAC clones contains the ELA class II region
and spans 800 kb. The class I and III regions are contained within the second contig
which has a 14 BAC clone minimum tiling path and spans 1.6 Mb. This study will
report on the construction of the two BAC contigs which span the ELA complex, and
characterization of the gene content and organization of the ELA complex.
14
Stokes, A. "Immune effector mechanisms in equine herpesvirus type-1 infection." Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233248.
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Guedes, Alves da Silva Adriana. "The Role of the CD14 molecule in equine endotoxemia." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/76807.
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Objectives - To evaluate the effects of equine sCD14 and monoclonal antibodies (mAbs) to equine CD14 on LPS-induced TNF° expression of equine peripheral blood mononuclear cells (PBMCs). To determine serum concentrations of soluble (sCD14) in a population of horses with gastrointestinal diseases or other illnesses likely to result in endotoxemia; and identify relationships with clinical data.
Animals - Part 1; 10 healthy horses. Part 2; 55 clinical cases and 23 healthy control horses.
Procedure - Part 1; PBMCs were incubated with Escherichia coli LPS, CD14 mAb, sCD14, CD14 mAb plus E coli LPS or sCD14 plus E coli LPS. Supernatants were collected at 6 hours and assayed for tumor necrosis factor ° (TNF°) activity. Part 2; Serum sCD14 was measured at admission and then at 24 and 48 hours after admission using a bead-based multiplex assay.
Results - Part 1; Pre-incubation with CD14 mAb did not inhibit LPS-induced TNF° protein production in isolated equine monocytes. Use of sCD14 inhibited LPS-induced TNF° protein production in isolated monocytes in a concentration-dependent manner. Part 2; Serum concentration of sCD14 was positively related to duration of clinical signs (P = 0.007), respiratory rate (P=0.04) and band neutrophil count (P = 0.0002). There was no correlation between serum concentration of sCD14 and heart rate, temperature, hematocrit, lactate, white blood cell count, fibrinogen, creatinine, urea nitrogen, glucose and anion gap values. Serum sCD14 did not correlate with outcome at any time point for clinical cases.Master of Science
16
Wright, Ashley D., and Elizabeth A. Greene. "Rabies in Arizona: Equine Risk and Prevention." College of Agriculture, University of Arizona (Tucson, AZ), 2016. http://hdl.handle.net/10150/608256.
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4 pp.The Informed Arizona Equestrian Horse Health Series was designed to bring up to date information on issues of importance to the horse owners of Arizona and beyond. Rabies has been identified in horses in Arizona (most recently Santa Cruz county in 2016), and is not only fatal for horses, but also can affect the humans handling those horses. It is often overlooked as a possible diagnosis due to the nonspecific early signs of infection. Find out how to protect you and your horses from this devastating disease.
17
Murrell, Joanna. "Spontaneous EEG changes in the equine surgical patient." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340352.
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Devine, Elizabeth P. "Pharmacokinetics of intramuscular morphine in the horse." Kansas State University, 2012. http://hdl.handle.net/2097/13864.
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Master of ScienceDepartment of Clinical Sciences
Warren L. Beard
Pharmacokinetics of Intramuscular Morphine in the Horse Elizabeth Devine, DVM; Butch KuKanich, DVM, PhD, DACVCP; Warren Beard, DVM, MS, DACVS Objective - To determine the pharmacokinetics of morphine after intramuscular administration in a clinical population of horses Design – Prospective, clinical study Animals – Pilot study included 2 normal horses and the clinical study included 75 horses Procedures – Morphine was administered at 0.1mg/kg, IM and 2-3 blood samples were obtained from each horse at various times from 0-9 hours after administration. Plasma morphine concentrations were measured using liquid chromatography and mass spectrometry. Results – Data was analyzed using a naïve pooled pharmacokinetic model. The half-life for the elimination phase was approximately 1.5 hours, the volume of distribution (per bioavailability) was approximately 4.5 L/kg and the clearance (per bioavailability) was approximately 35 mL/kg/min. The peak plasma concentration was 21.6 ng/mL and occurred approximately 4 minutes after administration. Plasma concentrations of morphine were below the limit of quantification by 7 hours in 74 horses. Conclusions and Clinical Relevance – The relatively short half-life of morphine indicates the need for frequent dosing to maintain targeted plasma concentrations. Adverse effects were uncommon in this study and morphine was well tolerated at a dose of 0.1 mg/kg, IM. Morphine may be a useful adjunctive therapy in painful horses, but the variable plasma concentrations suggest the dose and dosing interval may need to be adjusted to the individual patient’s response.
19
Sarrafchi, Amir. "Equine stereotypic behavior as related to horse welfare: A review." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-77944.
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There are strong suggestions that equine stereotypies are being connected to poor welfare and a sub-optimal management and/or stabling environment. Until today different forms of equine stereotypic behaviors have been described. Crib-biting, weaving, and box-walking are considered the most prevalent. Several studies have been conducted to establish links between the underlying causes and potential function of such behaviors. Both experimental and epidemiological studies have indicated management factors specifically feeding practices, housing conditions, and weaning method as crucial in the development of stereotypies in stabled horses. Some neurologic studies on equine stereotypy demonstrated some forms of CNS dysfunction as the causal factor for the performance of stereotypic behaviors. Different researchers hypothesized that the functional significance of stereotypies is that they reduce stress in captive environments and should thus be considered as a coping mechanism. In contrast, the owner‟s perspective is often that a stereotypic horse has a “stable-vice” that needs to be stopped and different kinds of methods have been developed to control or regulate stereotypic behaviors. However, if the stress-reducing hypothesis is correct, controlling stereotypic behaviors particularly by physical and surgical approaches without addressing the underlying causes is of great concern to the horse‟s welfare. Although there is ongoing uncertainty about the exact function, the growing knowledge about causation should be applied: under all circumstances prevention is better than cure.
20
Easeman, Richard. "Induction of mucosal immune responses in the horse." Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389312.
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Francis, Jesse. "SENSORY ANALYSIS OF EQUINE FEED PRODUCTS." OpenSIUC, 2020. https://opensiuc.lib.siu.edu/dissertations/1787.
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Three experiments were conducted to investigate factors that may influence horse and consumer preference of equine feed products. The first experiment was a two-phase study designed to investigate the impact of oil-based palatants on horse preference when topically applied to a pelleted diet. In Phase One, treatment diets containing six palatants (banana, anise, apple, peppermint, spearmint, and orange) were compared to a control diet (corn oil) in a paired preference test. Phase Two then compared three palatants (anise, apple, and peppermint) to each other. Preference testing was comprised of a 15 second olfaction period followed by a 3 minute consumption period and first diet sniffed, first diet consumed, first action, aversive behaviors, excessive salivation, amount consumed, and number of chews were recorded. Results from Phase One revealed that orange negatively impacted palatability indicated by less consumption when compared to the control (P = 0.02), though there was no impact on chews per gram. No difference between control and treatment diets for first sniff or first consumed was observed when analyzed individually in either Phase One or Two, though there was a moderate positive correlation (ф = 0.39, P = 0.04) between olfaction and consumption during the peppermint and anise comparison. Consumption as the first action was consistent across all trials (P < 0.05). Anise was preferred over apple and peppermint as indicated by higher total consumption (P < 0.05) in Phase Two. The second experiment was designed to compare horse and consumer preferences of two horse treats products. Horses were presented with two different treat products in a paired preference test comprised of separate olfaction and consumption periods. Additionally, consumers evaluated the two different horse treat products separately for purchase intent as well as consumer preference using a Hedonic ranking scale of the sensory attributes. Consumer data were analyzed by comparing 1) the preferences of horse owning participants to non-horse owning participants, and 2) horse owning participants preference for the two different treat products. No difference was observed for first product sniffed, consumed, or finished during the horse preference test. However, moderate positive correlations were observed between first product sniffed and consumed (P = 0.01, ф = 0.40) as well as first product consumed and finished (P < 0.01, ф = 0.48). Consumer testing revealed lower ratings for Product A in size (P = 0.01), texture (P = 0.02), and purchase intent (P = 0.02) from horses owners when compared to non-horse owners. Horse owners rated Product A lower in appearance (P < 0.01), texture (P < 0.01), size (P < 0.01), and purchase intent (P < 0.01) than Product B.The third project investigated the influence of packaging on shelf life stability and horse preference of treats. Three packaging treatments (control, poly, and paper) were examined at five time points over a 12-month period. Treatments were analyzed for moisture, water activity, mold, yeast, pH, and volatile organic acids. Horse preference testing evaluated first treatment sniffed, consumed, and finished as well as number of treats consumed. Moisture content and water activity increased in all treatments (P < 0.01) from month 0 to month 12, with paper packaging providing a greater fluctuation and containing visible mold at month 12 (P < 0.01). No difference was observed for first treatment sniffed, consumed, or finished during preference testing. However a trend (P = 0.09) for the period*treatment interaction was observed for number of treats consumed, with a poly increasing while paper decreased. These data indicate that 1) palatants and packaging material influence horse preference of feed products, and 2) both horse and consumer testing should be considered during product development to maximize acceptance.
22
McKlveen, Tori Leigh. "Evaluation of the Normal Equine Pituitary Gland." Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/10156.
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Computed tomography (CT) is becoming more available as a diagnostic tool in the evaluation of the equine skull and brain. Objectives of this study were: 1) refine a CT protocol for evaluating the equine pituitary gland, 2.) define the CT anatomy of the pituitary region, 3.) determine a set of normal values for the pituitary dimensions (length, width, height, volume and weight), 4.) refine CT techniques for measuring pituitary size.
Horses were scanned using 10x10mm, 10x5mm, 4x4mm and 4x2mm slice thickness and interval combinations. The pituitary glands were removed immediately after CT and gross measurements were performed. CT measurements were compared with gross pituitary measurements using analysis of variance (ANOVA) in a randomized block design. Accuracy percentages were also calculated using gross measurements as the known value.
Mean dimensions of the histologically normal pituitary glands were: length 21.07mm, width 21.62mm, height 9.78mm and volume 2.66cm³. The weights ranged from 1.7g to 3.4g with a mean of 2.6g. Computed tomographic measurement analysis demonstrated that the 10mm slices were the most accurate way to estimate the length of the gland. The 4mm slices yielded the highest accuracy values for width, height and volume of the pituitary gland. The volume was underestimated by all interval and slice thickness combinations performed by CT. No evidence of an overlap effect was identified for any of the dimensions.
Our findings indicated that contrast-enhanced CT is an accurate technique for estimating pituitary linear dimensions. Three-dimensional CT volumetry may not be an accurate method for estimating pituitary volume.Master of Science
23
Hewes, Christina Andrea. "Characterization of Apoptotic Cells in Equine Proximal Suspensory Desmitis." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/43303.
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Suspensory desmitis is a common problem and affects a broad cross section of equine athletes in various disciplines. For this study, the proximal portion of the suspensory ligament was collected from 6 horses without suspensory ligament injury (16 ligaments) and 4 horses with degeneration of the suspensory ligament (11 ligaments). Specimens were collected immediately after euthanasia and placed in neutral-buffered 10% formalin. The tissue was fixed, sectioned, and stained with hematoxylin and eosin (H&E),
Massonâ s trichrome, and for apoptosis by the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique. Histological changes in the abnormal ligaments included mineralization, fibroplasias, neovascularization, collagen degeneration, and significant architecture disruption in 2 ligaments. There was a trend for increased apoptosis in the injured ligaments compared to the normal ligaments.Master of Science
24
Murphy, David Matthew. "Studies on chronic gastrointestinal disease in the horse." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360253.
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Mellor, Dominic James. "A demographic and epidemiological study of the equine population of Scotland and northern England." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312459.
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Chavatte, Pascale Martine Bernadette. "Biosynthesis and function of corticoids and progestagens in equine pregnancy." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.264542.
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Hassan, Syed Saeed-Ul. "Rapid immunological methods for analysis of dexamethasone in equine urine." Thesis, University of Sunderland, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245822.
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Henderson, Cortney Erik. "Magnetic Resonance Imaging of the Normal Equine Larynx." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/33833.
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A study was performed to establish the appearance of normal equine laryngeal cartilages using magnetic resonance imaging. Specimens were acquired from clinically normal horses that were euthanized for reasons other than respiratory disease. Three in situ and 5 ex vivo larynges were imaged using a 0.3 Tesla system. Images were obtained in the transverse plane using T1-weighted 3D spin echo, T2-weighted 3D spin echo, T2-weighted gradient echo, short tau inversion recovery (STIR), and proton density spin echo sequences. Five ex vivo larynges were also imaged in the transverse plane using a 1.5 Tesla system, sequences included T1-weighted 3D spin echo, T2-weighted 3D turbo-spin echo, turbo inversion recovery (TIRM), and proton density spin echo sequences. A frozen gross laryngeal specimen was sliced in 5-mm transverse sections for comparison to the MR images. Excellent correlation was found between MR images and the gross transverse sections. Successful imaging was accomplished using both imaging systems; however, the 1.5 Tesla system yielded superior image resolution. The 0.3 Tesla imaging system would accommodate the intact equine head, which was not possible using the 1.5 Tesla MRI system. The internal morphology of the laryngeal cartilages was clearly identified in all imaging sequences obtained. Cartilages were found to differ in signal intensity based on the tissue composition and imaging sequences performed. MRI was determined to be a useful imaging modality for evaluating the cartilage morphology of the equine larynx. Further investigation is required to document pathologic morphology.Master of Science
29
Allen, Charlotte Annette. "Cytokine detection in EIAV-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction." Thesis, Texas A&M University, 2007. http://hdl.handle.net/1969.1/85887.
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The replication of equine infectious anemia virus (EIAV) in macrophages not only leads to cell death, but also to the induction of a variety of cytokines that may affect immune function. Cytokine production may be responsible for the fever, anorexia, hemorrhages, lethargy or thrombocytopenia seen in the acute and chronic phases of equine infectious anemia (EIA). The study of the equine immune system and inflammatory responses, by measuring cytokine expression, can provide important insight into disease pathogenesis in the horse. We have extended studies of virulent and avirulent EIAV clones by examining the effects of Env proteins on cytokine expression in equine monocyte-derived macrophages (EMDM) using EIAV17, EIAV19, EIAV17SU, and EIAV17TM viruses. In the current studies a set of quantitative real-time polymerase chain reaction (QPCR) assays for the equine cytokines IL-1a, IL-1b, IL-6, IL-8 and TNF-a were validated using QPCR primers and probes which were generated for the aforementioned equine genes.
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Wilsher, Sandra Ann. "Studies in equine reproduction." Thesis, University of Bedfordshire, 2009. http://hdl.handle.net/10547/134931.
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The papers put forward by the candidate represent a significant contribution to three main areas within the body of knowledge of equine reproduction. Namely, i) epidemiological surveys of the efficiency of Thoroughbred racing and breeding, ii) the morphology and functions of the equine placenta and, iii) embryo transfer in the horse. Two extensive surveys on reproductive efficiency of Thoroughbred mares and stallions at stud and factors associated with the failure of Thoroughbred horses to train and race demonstrated that increasing mare age is the greatest limiting factor to an otherwise high rate of fertility in English Thoroughbreds although a high incidence of early embryonic death remains a significant loss to the breeding industry. The racing wastage survey showed little change over the past 20 years in the percentage of 2- and 3-year-old horses that fail to run, the percentage that are never placed in a race and the number that suffer significant injury or illness during their racing careers. Radical and innovative changes to training methods are needed to overcome these problems. The morphology of the equine placenta was examined using gross measurements, stereological-techniques, vascular casting and immunohistochemistry and the findings related to fetal development and postnatal growth. Stereological measurements applied to term placentae established reference parameters such as surface area per unit volume of placental microcotyledons, the total microscopic area of contact between mother and fetus at the placental interface, and placental VI efficiency. Maternal age, parity, size, genotype and nutrition were all shown to alter placental morphology and, hence, pre- and postnatal fetal development. A novel pair of cervical forceps were designed and marketed to provide a simple and practical method for undertaking transcervical embryo transfer in the horse which enables inexperienced operators to transfer horse embryos successfully. These Wilsher Equine Embryo Transfer Forceps have won widespread acclaim and commercial application in the equine veterinary and scientific communities. A pharmacological method to extend donor-recipient synchrony was developed with both commercial and scientific application. Further work also showed the unique ability of the equine embryo to tolerate a very wide window of donor-recipient asynchrony and it provided a valuable research tool with which to study the relevant roles of the conceptus and uterine environment in regulating embryonic differentiation and fetal growth in the mare.
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Suagee, Jessica Kanekakenre. "Regulation of Nutrient Metabolism in Equine Skeletal Muscle and Adipose Tissue." Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/40294.
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Glucose and lipid metabolism are dysregulated in obese horses. Altered glucose metabolism is evidenced by the development of insulin resistance and increased fasting plasma insulin concentrations (hyperinsulinemia) while altered lipid metabolism is evidenced by increased plasma lipid concentrations. Obesity in horses also increases the risk of the painful hoof disease, laminitis. Three experiments were performed to investigate the regulation of nutrient metabolism in skeletal muscle and adipose tissue of lean, healthy horses. Adipose tissue was found to be the primary lipogenic tissue of horses, with acetate being the primary lipogenic substrate. Secondly, ten, lean horses were used to investigate the effects of acute hyperinsulinemia on nutrient metabolism. Increasing plasma insulin concentrations to >1,000 mIU/L for six hours decreased transcript abundance of glucose transporters and the insulin receptor in adipose tissue, and decreased protein abundance of the insulin receptor in skeletal muscle, potentially indicating that hyperinsulinemia potentiates insulin resistance. Insulin infusion also reduced mRNA abundance of lipid transporters in adipose tissue while increasing them in skeletal muscle. The final experiment investigated the influence of the insulin-sensitizing drug, pioglitazone, and lipopolysaccharide, on nutrient metabolism in skeletal muscle and adipose tissue, and their association with insulin sensitivity. Pioglitazone treatment did not increase insulin sensitivity; however it did increase skeletal muscle transcript abundance of the insulin receptor and the non-insulin sensitive glucose transporter and adipose tissue protein abundance of the insulin-sensitive glucose transporter (GLUT4). Lipopolysaccharide decreased insulin sensitivity regardless of pioglitazone pre-treatment, which was associated with decreased transcript abundance of GLUT4
in skeletal muscle and adipose tissue of untreated horses, but not adipose tissue of pioglitazone treated horses.Ph. D.
32
Goehring, Lutz Steffen. "Equine Protozoal Myeloencephalitis. Preliminary Investigation of Protozoan-Host interactions in the horse." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/45145.
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Equine Protozoal Myeloencephalitis is the most frequently diagnosed neurologic disorder of horses in the united states, which is caused by the protozoan organism Sarcocystis neurona. The disease has a profound impact on the American Horse Industry. This impact includes prolonged and expensive treatment without a guaranteed return to a previous level of use for the individual horse. Poor respponse to and prolonged duration of treatment may suggest an immune mediated impariement of host response. There is limited information about the direct interaction between the pathogen and the host.
In two in vitro experiments we investigated a) whether the presence of the protozoan organism can influence mitogen-stimulated peripheral blood mononuclear cells (PBMCs), suggesting a direct influence of the protozoan organism on cells of the immune system, and b) if cerebrospinal fluid (CSF) from horses with EPM has an effect on mitogen-stimulated PBMCs, suggesting that the microenvironment of the site of infection influences the course of disease.
Experiment 1: Mitogen simulated PBMCs from EPM affected and control horses were co-cultured with fragments of freeze thawed bovine turbinate cells that were infected with S. neurona merozoites. Compared to controls PBMCs co-cultured with S. neurona fragments were the only cells that showed a decreased proliferation (p<0.05). A difference between EPM affected and control horses could not be detected (p>0.05). These results may imply that the persistence of S. neurona infection in the horses CNS is, in part, due to a pathogen-derived mechanism that attentuates the hosts immune response.
Experiment 2: Mitogen stimulated PBMCs from a horse affected with EPM and a control were co-cultured n the presence of CSF from EPM affected and uninfected controls. Prior to co-culture the CSF was fractionated by a filtration process over two microfilter units. An identical volume of NaCl (0.9%) served as a control for the volume of CSF that was added. The proliferation assay revealed a deviation of the response depending on cell donor and CSF fraction used. The effect was independant of the protein concentration of the CSF fraction, and a decrease in lymphocyte proliferation was not caused by increased cellular death. This suggests the presence of subsets within the CSF which have a stimulatory of suppressive influence on the cells in culture. The effect was cell donor dependant which implies a difference in lymphocyte subsets between the two horses that were used.Master of Science
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Archer, Debra Catherine. "Horse and management level risk factors for specific types of equine colic." Thesis, University of Liverpool, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440775.
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Lea, Richard Graham. "The identification of biological activities of pregnancy-associated proteins of the horse." Thesis, Sheffield Hallam University, 1988. http://shura.shu.ac.uk/20161/.
Full textAbstract:
Antisera were raised in rabbits against serum samples taken from pregnant and non-pregnant mares. Using these antisera in two-dimensional crossed immunoelectrophoresis, a protein (beta2-hors.PP) was found to be pregnancy specific and first detectable in the maternal circulation 6 days after mating. beta2-hors.PP detection as a pregnancy test during the first 21 days after mating was found to be significant at the 10% level. Levels of the protein were also found to increase during this period. The protein, of possible uterine origin, may have a biological activity associated with implantation (day 37) as levels of beta2-hors.PP were elevated from around days 21 to 83. For the remainder of gestation, the concentration of beta2-hors.PP in the serum decreased. It is possible that beta2-hors.PP detection may be a means of monitoring foetal well being. Equine epitheliochorial placental extracts, taken from 2 mares 60 days and 80 days after mating, were found to induce a dose-dependent inhibition of lymphocyte proliferation in the mixed lymphocyte reaction (MLR) and mitogen stimulated assay (MSA), MLR supernatant reversed the inhibition and the addition of foetal tissue extracts to previously activated lymphocytes suppressed lymphocyte proliferation. This suggests suppression at the IL-1/IL-2 level of the immune response. Commercially available eCG inhibited lymphocyte proliferation. The presence of endometrial cup secretion containing high levels of eCG had no additive effect on the inhibitory activity of an extract of allantochorion (day 80), previously shown to induce less than 100% inhibition. This suggests that the commercially available eCG may contain an immunosuppressive contaminant.Equine and bovine epitheliochorial placental extracts (allantochorion) inhibited collagen and ADP-induced platelet aggregation respectively. The mode of action of the anti-aggregatory factors may be related to the vascularity of the placentae. Overall, it appears that local immunomodulatory and haemostatic mechanisms are important in the maintenance of pregnancy in species with epitheliochorial placentation.
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Francis, Jesse M. "THE EFFECTS OF HYDROPONIC WHEAT FODDER ON FECAL METABOLITES IN EQUINES." OpenSIUC, 2017. https://opensiuc.lib.siu.edu/theses/2096.
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The use of hydroponic feeding systems for horses has gained in popularity during recent years. Typically, this feeding system allows for a more efficient use of the whole plant, including the shoot, root, and seed remnants rather than traditional grazing in which only the shoot of the plant is consumed. Vertical systems have practical uses in largely developed areas where traditional forage sources are limited, in arid countries or in areas with severe droughts where forage growth is minimal. Though there is some research on fodder utilization in production animals, there are currently no published data on the effects of fodder in horses. Our study, approved by the Southern Illinois University Institutional Animal Care and Use Committee (#13-043) utilized eight Quarter Horse mares randomly assigned to one of two diets. Control (CON) horses were offered 2% of their body weight (BW) in hay (DM) and treatment (TRT) horses received 1% of their BW in hay (DM) and 1% of BW in fresh wheat fodder (AF) twice daily. Body weight and hoof temperature data were recorded weekly. Fecal samples were collected weekly and analyzed for pH, NH3, and VFA concentration as well as DM, ash, NDF, ADF, N, CP, and EE. Hay and fodder samples were also collected weekly to monitor nutrient profiles of the two forage types for the duration of the study. Additionally, nutrient profiles from seed to mature (8 d growth) were developed for fodder. Data were analyzed as a completely randomized design using PROC MIXED of SAS (v. 9.4) and significance was established at P < 0.05. There were no significant differences in body weight, left or right front hoof temperatures between treatments. Fecal pH was significantly lower (P ≤ 0.01) in the TRT when compared to CON, and isobutyric acid was significantly (P ≤ 0.05) higher in TRT as compared to CON. A comparison of the nutrient values of the two forages demonstrated significantly higher DM, ash, NDF, and ADF (P <0.0001) in hay while N, CP, and EE (P <0.0001) were significantly higher in fodder overall. Daily growth of the fodder decreased DM content (P <0.0001) while ash, NDF, ADF, N, CP, and EE (P <0.0001) increased as the fodder reached maturity. These results indicate that utilizing fodder affects fecal metabolites associated with digestion.
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Young, Byron Leslie. "Treatment of Ischemic Equine Jejunum with Topical and Intraluminal Carolina Rinse." Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/34012.
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Carolina Rinse (CRS) has been shown to be effective in decreasing vascular permeability and neutrophil infiltration in reperfused equine small intestine. The objective of this study was to show that CRS applied topically and intraluminally could prevent immediate reperfusion injury after low flow ischemia or distention in the equine jejunum.
Materials & Methods: Two groups of 5 horses were used to evaluate CRS treatment after low-flow ischemia (Group 1) and intraluminal distention (Group 2) of distal jejunum. Mesenteric blood flow, osmotic reflection coefficient (ORC), wet weight to dry weight ratios (WW/DW), and neutrophil accumulation in the serosa were measured. ORC is defined as the lymph protein concentration to plasma protein concentration ratio subtracted from one (1- Cl / Cp) at maximal lymph flow. The ORC from baseline values and at 60 minutes after initiating reperfusion was compared between Groups 1 and 2. Pair wise comparisons were made for mesenteric blood flow, tissue volume, neutrophil number, and WW/DW proximal control and CRS treated jejunal segments were made using a Mann Whitney U test (P< 0.05).
Results: The mean ORC of bowel treated topically and intraluminally with CRS was similar to that recorded in normal bowel or ischemic intestine treated with CRS by arterial perfusion. The ORC after distention and decompression increased and was similar to that reported in untreated intestine. The WW/DW after both ischemia and distention increased compared to the proximal control segments. There was no difference in neutrophil number in either ischemic or distended intestine compared to the proximal control segments.
Discussion: Carolina CRS was effective in preventing alterations in microvascular permeability during reperfusion afterischemia but not distention. Neutrophil migration curtailed in both groups suggesting that combined topical and intraluminal application of CRS to ischemic intestine may reduce the acute inflammatory responses during reperfusion thereby decreasing complications after ischemia or distention.Master of Science
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Liuti, Tiziana. "Morphological assessment of paranasal sinuses and teeth in the horse." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/33192.
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Sinonasal and dental diseases can be a serious disorder in horses due to the persistence of the former and the common extension of the latter to the supporting bones of the skull. The diagnosis of equine sinonasal and dental disease can be challenging due to the complexity of these and adjacent anatomical structures, and therefore the use of advanced imaging, including computed tomography (CT) has greatly increased recently. A major aim of this study was to improve the morphological characterization of the sinuses and cheek teeth in normal horses of different ages. These studies defined the volumes of the 7 different sinus compartments; showed that the volume of the different compartments increase with age; that in contrast to accepted findings, the Triadan 09 alveoli is located in the caudal maxillary sinus (CMS) in 13% of the youngest group and the Triadan 10 alveoli is fully within the rostral maxillary sinus (RMS) in 53% of cases. The infraorbital canal was found to be susceptible to apical infection-related damage in horses < 2 years of age (< 2 y.o.) due to the intimate relationship of this canal and the medial aspect of the maxillary cheek teeth alveoli. The mean positions of the clinical crowns and apices of the maxillary Triadan 11s were 2.48 cm (adjusted 5.2% of head height) and 2.83 cm (adjusted 6.2% of head height) more rostral, respectively in horses >16 y.o. compared to horses < 6 y.o., consistent with rostral dental drift. Measurements of dental drift could provide further objective radiographic guidelines on ageing horses by dental imaging and also help our understanding of the aetiopathogenesis of cheek teeth diastemata. This study also found that the reserve crown length decreased from a mean of 4.7 cm (adjusted 19.7% of head height) in the youngest (< 6y.o.) group of horses to a mean of 2.9 cm (adjusted 10.5% of head height) in the oldest group (>15y.o.), with the 06s and 09s having the shortest reserve crowns. These data are useful reference information for clinicians and radiologists in the diagnosis and treatment of equine sinonasal and dental disease. Morphological characterization of the ventral and dorsal conchal bullae in horses of different age using CT described the age-related difference in sizes of these structures (smaller in younger horses). In the ventral conchal bulla, this reduction in volume was probably related to protrusion of the large dental alveoli in young horses into the nasal cavity and bulla. Bullae linear measurements and volumes were also associated with head size. The anatomical position of both bullae was associated with specific maxillary cheek teeth, thus increasing the diagnostic value of conventional radiography in the investigation of bulla disease and providing landmarks for the surgical treatment of this disorder. Skull shape analyzed with the use of Procrustes statistical analysis showed landmark variability between different age groups and in particular, high landmark variability between young (< 5y.o.) and old (>16y.o.) horses and less landmark variability between adult (6-15y.o.) and old horses. Future studies could investigate changes in the equine skull in relation to genotype to characterize breed-related diseases affecting teeth and sinonasal compartments. In the second part of the study, 32 infected maxillary cheek teeth extracted from clinical cases were evaluated grossly and histologically and these pathological results were compared to the pre-extraction radiographic and CT imaging findings. This study showed a high sensitivity (97%) of CT in detecting changes in teeth affected by apical infection. In the third part of the study, 30 cheek teeth assessed as being abnormal based on oral, radiographic and CT examinations were extracted along with their alveoli from 27 cadaver heads. Gross and histological examination findings from these teeth and alveoli were compared to the pre-extraction imaging findings, again showed high sensitivity (96.4%) of CT in diagnosing cheek teeth apical infection. Additionally, CT showed a high correlation with histology in detecting dental infection related alveolar bone changes including alveolar bone lysis, sclerosis and bone thickening. This aspect of the study was also the second largest pathological study of equine apical infection published to date and thus has also improved our understanding of the pathogenesis of this disorder. In conclusion, CT was shown to be an excellent imaging modality for improving the morphological characterization of the sinuses and teeth in normal horses of different ages, and thus also improving the value of this modality in diagnosis and treatment of disease affecting sinonasal and dental structures. This study also objectively showed the high sensitivity of CT in detecting cheek teeth apical infection in horses as compared to radiography, including by assessment of alveolar bone changes. Overall, the results of this project improve morphological knowledge of the equine skull and teeth and will have direct clinical benefits by improving the diagnosis and thus the treatment of equine sinonasal and dental disease.
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Hay, Alayna Nicole. "Equine Protozoal Myeloencephalitis: investigating immunopathogenesis and treatment efficacy in mouse models and clinically affected horses." Diss., Virginia Tech, 2020. http://hdl.handle.net/10919/96373.
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Equine protozoal myeloencephalitis (EPM), predominantly caused by the protozoa Saracocystis neurona, is a common neurologic disease in horses from North America. Equine exposure to the parasite occurs frequently as the protozoa is excreted in opossum (Didelphis virginiana) feces and contaminates the horse's environment. However, clinical neurologic disease only emerges in a small fraction of exposed horses. The seemingly protective immune response that develops in some exposed horses but not all is not fully defined. Previous reports utilizing horse EPM models and immune compromised mouse models, which develop disease simulating EPM after infection with S. neurona, have reported a role of T-lymphocytes and the cytokine interferon gamma, in disease protection. As part of this dissertation, the role of T-lymphocytes and IFNγ was further elucidated. It was determined that IFNγ production is essential for T-lymphocytes to offer protection against S. neurona induced encephalitis, in immune compromised mice. Another factor hindering prognosis of EPM affected horses is treatment failure. The efficacy of the antiprotozoal decoquinate, was tested and found to be ineffective at preventing S. neurona encephalitis, in immune compromised mice. However, the antiprotozoal, diclazuril, was found to be effective at preventing S. neurona encephalitis in immunocompromised mice but once treatment was terminated, infection persisted, and neurologic disease developed. In-situ methods were employed to extensively evaluate the immunopathology of spinal cord tissue samples collected from EPM affected horses. A novel in-situ hybridization technique was successfully utilized to identify S. neurona in tissue samples collected from horses with EPM. This technique will create new opportunities for investigating the immunopathology of EPM. Overall results from the studies conducted in this dissertation suggest that IFNγ production from T lymphocytes is essential for them to offer protection against S. neurona encephalitis. Additionally, further insight on FDA approved and non-FDA approved treatment options for S. neurona infection was gained through the use of the B6Ifnγ -/- mouse model. Collectively, these studies expanded on the knowledge of an understudied equine neurologic disease.Doctor of Philosophy
Horses are susceptible to the neurologic disease Equine Protozoal Myeloencephalitis, more commonly referred to as EPM by equine enthusiasts. The disease results from ingestion of the parasite, Saracocystis neurona, which contaminates the horse's natural environment; therefore, horses are likely to come in contact with the parasite while eating or drinking. Not all horses that encounter S. neurona develop neurologic disease, some will be protected by their immune system with the only evidence of exposure being serological antibodies. In efforts to not experimentally induce EPM in horses, an immunocompromised mouse model is often used instead. Through the use of the immunocompromised mouse model, researchers have discovered that the immune cell, T lymphocytes, and signaling molecule, interferon gamma, are important for protection against S. neurona infection. In one study conducted for this dissertation it was found that T lymphocytes need to be able to produce interferon gamma in order to provide protection. Another issue that the immunocompromised mouse model has helped address, is EPM treatment efficacy. The inability of antiprotozoal drugs that are utilized for EPM treatment to fully eliminate the parasite from the horse's body is thought to cause reoccurring disease in some horses. One non-FDA approved treatment was evaluated here and determined not to be effective in the immunocompromised mouse model. One FDA approved treatment option, which is commonly used to treat EPM, was evaluated as well. This drug was proven to be effective at preventing disease while mice were being treated but termination of treatment led to development of neurologic disease, exemplifying treatment failure. One final study was conducted to examine the different types of immune cells and signaling molecules in spinal cord tissue samples collected, from horses which had to be euthanized due to poor prognosis related to EPM. In this study a novel experimental technique was successfully used which will help progress EPM research. Overall results of these studies offered more explanation on the immune response that protects against neurologic disease from S. neurona infection and demonstrated that not all treatments are effective and reoccurring disease may be a result of treatment failure.
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Cissell, James Michael. "In Vitro Equine Flexor Tendonitis: New Model Development and Therapeutic Investigation." Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/34608.
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Flexor tendonitis is a common cause of lameness and wastage in the equine athlete. Current techniques for tendonitis therapy provide limited success, and horses that do recover tend to return at a decreased level of performance. Current treatment techniques have begun to focus on regenerative medicine to improve tissue healing. Investigations of new treatments are made difficult by the lack of reliable in vitro models that allow for accurate comparison of treatment protocols. New techniques are often implemented into the clinical setting prior to thorough investigation for safety and efficacy.
In vitro testing is an important step in the development of new therapeutic agents. However, results of in vitro tests should only be deemed as useful if the model used is one that is reliable and mimics the clinical situation that the reseachers are attempting to investigate. Equine flexor tendonitis is believed to be the result of microdamage caused by cyclic loading of tendons. Cyclic loading of fibroblasts results in increased production of the inflammatory cytokine prostaglandin E2 (PGE2). Thus the exposure of tendon fibroblasts to exogenous PGE2 may induce metabolic changes in the cells similar to what is seen in clinically affected animals making this a useful model for the investigation of therapeutic techniques.
Currently a variety of techniques exist for treatment of flexor tendonitis; however, no single treatment has separated itself as superior. A new technique using autogenous conditioned serum (ACS) in humans for treatment of muscle injury has been shown to speed tissue regeneration. ACS produced from human blood has been shown to contain significantly increased levels of III growth factors that may improve tendon fibril formation and strength. We propose to investigate the effect of ACS on cellular metabolism in equine tendon fibroblast monolayers. This will involve cell culture, PGE2-induced cellular injury, and analysis of the cellular response to injury when treated with ACS. Controls will include fetal bovine serum, normal equine serum, and ACS without PGE2-induced cellular injury. The cellular response will be investigated biochemically by quantification of DNA, glycosaminoglycan, and soluble collagen levels and by real time PCR to assess gene expression for matrix metalloproteinases (MMP)-1, MMP-3, and MMP-13, collagen types I and III, and the non-collagenous proteins cartilage oligomeric matrix protein (COMP) and decorin. Data will be analyzed by analysis of variance and post-hoc comparisons. Significance will be set at p<0.05.
We hypothesize that the addition of exogenous PGE2 to culture media for monolayers of equine tendon fibroblasts will insight alterations in cellular metabolism that will generate a suitable model for the in vitro study of fibroblast response to novel therapies. We then hypothesize that the addition of ACS to PGE2-treated fibroblasts will result in increased gene expression for collagen types I and III, cartilage oligomeric matrix protein, and decorin. ACS will also stimulate increased protein production of collagen and glycosaminoglycans, and stimulate increased cell proliferation. The use of ACS will decrease gene expression of inflammatory molecules important in tendon degradation, namely matrix metalloproteinases -1, -3, and -13.Master of Science
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Holdorf, Colette Rose. "Intimacy and Investment in Canterbury Thoroughbred Horse Racing: A Study in Equine Anthropology." Thesis, University of Canterbury. School of Social and Political Sciences, 2013. http://hdl.handle.net/10092/8308.
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This thesis is concerned with equine attachment as a form of interspecies fictive kinship, in relation to the practices, social relations, and motivations of participant groups within the Canterbury thoroughbred racing industry. Exploring the interconnections between intimacy and investment, as both financial and emotional commitments, the different sectors of the industry are analyzed as actor-network assemblages in which boundaries between professional, economic, and social relations are porous. The thesis argues that the parameters of a distinct equine community are delineated through regulated forms of haptic access. Distinctions regarding those who are and are not permitted to touch horses, articulates with a regime of risk regulation, which is more broadly explored in its physical, social, financial, and emotional dimensions. Also integral to the character of this networked community of practitioners is the symbolism of naming and branding thoroughbreds, as well as the secular rituals of the auction and the race-day. Conceived as a naturalcultural phenomenon, the race is analyzed in terms of the cross-species meanings and experiences of jockeys and apprentices, who are understood as representatives of an occupational sub-culture. Finally, this thesis also explores the preponderance of women in South Island racing; charting the path by which they have successfully adopted traditionally male-dominated roles.
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Wereszka, Marta. "Methods to Detect Apoptosis in Equine Peripheral Blood Neutrophils from Normal Healthy Adult Horses." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/33781.
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Apoptosis is a form of "planned cell death" and is an essential component of normal tissue differentiation and functional regulation. Neutrophil apoptosis facilitates down regulation of the inflammatory response while minimizing "by stander" injury to normal tissue, and disruption of this process by various diseases may have a significant negative impact on patient recovery. Consequently, neutrophil apoptosis has been the focus of research in many species. However, methods for measuring apoptosis have not been evaluated in the horse. The goal of this study was to adapt previously reported methods for inducing and measuring both neutrophil apoptosis and necrosis in non-equine species for use in equine peripheral blood neutrophils.
To achieve this goal the experiment was divided into three parts: 1. Induce apoptosis and necrosis in equine peripheral blood neutrophils using previously used known inducers and examine the relationship between exposure time and percentage of affected cells; 2. Measure percentage of apoptosis and necrosis using three methods of detection: a) Annexin-V Fitc PI assay, b) Homogenous caspase 3/7 assay and c) Light microscopy and; 3. Compare the results between the three methods of apoptosis detection to determine if results are comparable
The hypothesis was that previously reported methods for inducing and measuring both neutrophil apoptosis and necrosis in non-equine species can be adapted for use in equine peripheral blood neutrophils.
Venous blood samples were collected aseptically from the jugular vein of eight horses. Isolation of neutrophils was performed using density gradient centrifugation on percoll. In part 1 of the experiment aliquots of the neutrophil suspension were cultured in the presence of four known inducers of apoptosis; actinomycin D, staurosporin, cycloheximide and sodium hypochlorite, at four different concentrations (table 2). A fifth population was to induce necrosis using a freeze-thaw cycle and bleach. A control sample was examined (no inducer) to determine spontaneous rate of apoptosis. The aliquots were cultured and the percentage of apoptosis determined at two sequential time points for each horse. Apoptosis was measured at either 30 minutes and 3 hours or 6 and 12 hours by three simultaneous methods: (1) annexin-V FITC PI assay (AVF), (2) homogenous caspase assay (HC) and (3) light microscopy (MS). The AVF and HC methods detect events associated with early apoptosis whilst MS detects nuclear changes which are late events of apoptosis. Using AVF and MS apoptotic cells are able to be differentiated from necrotic cells.
In part two of the experiment the agreement and reproducibility between AVF and MS was further examined. In this part of the experiment neutrophils were isolated from the peripheral blood of 10 normal healthy adult horses. Each isolated sample was cultured with 80µM Actinomycin D for 12 hours and a control sample (no inducer) also prepared. Three triplicate samples were next set up from both the induced and control sample and apoptosis was determined using both AVF and MS.
In part 3 of the experiment, data was analyzed using the mixed model ANOVA following log transformation of the data. Main effects of treatment, concentration and time were analyzed. Statistical significance was considered if P was < 0.05.
The relationship between the three techniques; light microscopy, flow cytometry and the fluorescent plate reader, was investigated using Spearman rank correlation coefficients (Fisher's Z transformation). The Bland-Altman approach for method analysis was used to further characterize the correlation between results obtained via light microscopy and flow cytometry. Statistical significance was considered if P < 0.05.
All inducers increased the percentage of apoptotic cells at either one or more time point and results were most comparable between AVF and MS. Increasing exposure time increased percentage of apoptotic neutrophils for all inducers using AVF and MS (p<0.0001). For both AVF and MS, cycloheximide and staurosporin induced apoptosis significantly above control levels at 3, 6 and 12 hours; actinomycin D at 6 and 12 hours and bleach at 3 and 6 hours as well was 12 hours for AVF only. With HC induction of apoptosis was detected earlier with bleach at 30 minutes and 3 hours and staurosporin at 30 minutes, 3 and 6 hours. Apoptosis was detected only at 6 hours for cycloheximide.
Increasing concentration of inducer significantly increased the percentage apoptotic cells for staurosporin and cycloheximide between the lowest and highest concentration using AVF (p<0.001). For both AVF and MS, increasing concentration of bleach decreased the percentage of apoptotic cells (p<0.05). Increasing the concentration of staurosporin resulted in an increase in apoptosis at 30 minutes and 3 hours.
Both bleach and the freeze-thaw cycle induced necrosis at all time periods excluding 30 minutes for the freeze-thaw cycle (p<0.0001).
Spearman rank correlation coefficients revealed a very high correlation for percentage apoptosis and necrosis between AVF and MS (r2 = 0.91, 95% CI 0.89 – 0.93). A high correlation was also present for AVF and HC (r2 = 0.75, 95% CI 0.69 – 0.79) and MS and HC (r2 = 0.76, 95% CI 0.71 – 0.81). The lower limit of the confidence intervals suggests there is some concern about the similarity between AVF, HC and MS, HC.
The Bland and Altman statistical approach indicates that both AVF and MS are highly reproducible methods with minimal variation between the triplicate samples (AVF: 8.9%, 95% CI 6.25 – 11.6%, MS 7.9%, 95% CI 6 – 9.8%). The mean difference between the two methods is 6.7% (95% CI 3.89 – 9.42%). The 95% limits of agreement indicate that results from MS can be 8.7% below to 22% above results from AVF (95% CI -13.41 – 26.7%).
These findings indicate that caspase activation may occur prior to phosphatidylserine externalization and visible nuclear changes, which is in accordance with previously published data. We discovered that actinomycin D induces significant and reproducible equine peripheral blood neutrophil apoptosis in a time dependant fashion. Similarly, necrosis results from a freeze-thaw cycle or high concentration of bleach and is suitable as a positive control for necrosis. Apoptosis was effectively detected using AVF assay and results indicate good correlation between AVF and MS with an acceptably low mean difference. MS could serve as an inexpensive, simple and quick on site method to rapidly verify results attained from AVF. Induction of apoptosis using the HC was not consistent and can not be recommended based on the results of this study. Future investigation aimed at evaluating assays multiplexed to the AVF which detect other aspects of the apoptotic pathway would lead to increased confidence of results and further evidence of the mode of cell death prior to undertaking clinical studies.Master of Science
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Stuska, Susan Jolene. "Needs-based curricular content goals for two-year equine curricula." Diss., This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-08032007-102241/.
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Proudman, Christopher John. "An epidemiological investigation of the role of the equine tapeworm Anoplocephala perfoliata in the aetiology of intestinal disease." Thesis, University of Liverpool, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320499.
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Jones, Angela Jane. "Normal and diseased equine digital flexor tendon : blood flow, biochemical and serological studies." Thesis, Royal Veterinary College (University of London), 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324895.
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Teegerstrom, Trent, William A. Schurg, Kelly Block, and Mark Arns. "Thinking of Owning a Pleasure Horse? A Guide for the Care and Ownership of a Pleasure Horse in Arizona." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2004. http://hdl.handle.net/10150/144714.
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22 pp.Private horse ownership is not for everyone; owning a horse comes with many responsibilities. You must properly house and care for the horse. This care includes the horse's feeding, health care, and hoof care, but these are only part of the equation. You must also provide housing facilities, transportation, and riding equipment. This booklet is an introductory guide to the proper care and cost of owning and maintaining a pleasure horse in Arizona. We discuss how to feed and care for a horse as well as all of the associated costs to expect whether you board your horse or house and care for it privately. The publication has two major sections: (1)maintaining and caring for a horse, and (2) budgeting for the costs of ownership and care. There is also an introduction to getting started and a list of additional resources at the end.
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Toukonen, Margaret Cuffari. "The relationship between adolescent girls and horses: Implications for equine-assisted therapies." Kent State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=kent1308581549.
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Moore, Tabitha Gale Bryant. "Functional characterization of equine neutrophils in response to calcium ionophore A23187 and phorbol myristate acetate." Thesis, This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-06102009-063430/.
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Sullivan, Erin Alene. "Channels and sources used to gather equine-related information by college-age horse owners and enthusiasts." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-3096.
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Murphy, Barbara Anne. "INVESTIGATIONS OF CIRCADIAN REGULATION AND IMMUNE-CIRCADIAN INTERACTION IN THE HORSE." UKnowledge, 2007. http://uknowledge.uky.edu/gradschool_diss/546.
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The circadian system provides animals with a means to adapt internal physiology to the constantly changing environmental stimuli that exists on a rotating planet. Light information is translated into molecular timing mechanisms within individual pacemaker cells of the mammalian hypothalamic suprachiasmatic nucleus (SCN) via transcriptionaltranslational feedback loops. Humoral and neural outputs from this master clock result in circadian rhythms of physiology and behavior. The hierarchy of the circadian system involves SCN synchronization of cellular clocks within peripheral tissues so that differential transcriptional profiles in individual organs reflect their specific function. The first step to investigating equine circadian regulation was to identify and isolate the core components of the molecular clock in the horse. Successful isolation and sequencing of equine Bmal1, Per2, Cry1 and Clock cDNAs revealed high sequence homology with their human counterparts. Real Time RT-PCR assays were subsequently designed to quantitatively assess clock gene expression in equine peripheral tissues. Synchronization of equine fibroblasts revealed temporal profiles of clock gene expression identical to those of the SCN and peripheral tissues of other species. However, while clock gene expression varies over time in equine adipose tissue, there was no observable oscillation of clock gene transcripts in equine blood. Spurred by recent reports of immune-circadian interactions, this novel finding prompted an investigation of clock gene expression in equine blood during a systemic inflammatory response. The results demonstrated that acute inflammation upregulates Per2 and Bmal1 in equine blood. Subsequent experiments identified neutrophils as the source of this upregulation and highlighted exciting new immunecircadian interplay during an innate immune response. Finally, the effect of a 6-h phase advance of the light/dark cycle, mimicking an easterly transmeridian journey, on circadian melatonin and core body temperature rhythms was investigated. In contrast to the gradual adaptation observed in other species, these markers of equine circadian phase adapt immediately to a time zone transition. Combined, the results of these experiments highlight important interspecies differences in circadian regulation with practical implications regarding the potential impact of jet lag on equine athletes. Furthermore, the results underline the relevance of chronobiological investigation in a large mammalian species such as the horse.
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Antunes, Gastal Gustavo Desire. "Fertility preservation of ovarian germ cells: the horse and deer models." OpenSIUC, 2016. https://opensiuc.lib.siu.edu/dissertations/1295.
Full textAbstract:
Preserving viability of frozen gametes and reproductive tissues is crucial to understand and overcome species-specificities in respect to the diversity in cryobiological properties and requirements among cell types and tissues. The use of different animal models to study ovarian tissue cryopreservation will help to uncover several important factors related to germ cells preservation. Horses (Equus ferus caballus) have been proven to be an excellent model for reproductive biology studies with implications for humans. White-tailed deer (Odocoileus virginianus) are one of the most abundant wild species in the United States, but little information about their reproductive features are known. Therefore, five studies were conducted in this Dissertation with the following general objectives: (i) to develop ovarian tissue cryopreservation techniques for horses and white-tailed deer species; and (ii) to determine the effects of ovarian tissue cryopreservation techniques on morphological and molecular mechanisms related to folliculogenesis in horse and white-tailed deer species. In study one, equine ovarian tissue was used to determine the ideal ovarian fragment size for better cooling resistance under storage at 4°C. In study two, equine ovarian tissues were used to determine the toxicity effect of cryoprotective agents on ovarian tissue pre- and post-cryopreservation. In study three, equine ovarian tissues were used to compare slow-freezing versus vitrification; and to determine the best cryoprotective agents for each cryopreservation method. In study four, white-tailed deer reproductive tracts were used to characterize the age effect on reproductive features. In study five, white-tailed deer ovarian tissue was used to compare slow-freezing versus vitrification methods to preserve preantral follicles under in vitro culture. The main findings of the horse studies were: (i) equine ovarian tissue can be stored at 4°C for up to 24 h when biopsy ovarian fragments are used; (ii) ethylene glycol seems to be a less harmful cryoprotectant agent to equine preantral follicles; and (iii) both slow-freezing and vitrification methods similarly preserved the follicle morphology after time of culture. The main findings of the white-tailed deer studies were: (i) aging caused quantitative and qualitative effects on the ovarian reserve of white-tailed deer; (ii) fresh ovarian tissue can be cultured for up to seven days preserving the tissue integrity; and (iii) fragments cryopreserved by vitrification had higher follicle viability during in vitro culture than by the slow-freezing method. In conclusion, this work demonstrated the viability to cryopreserve equine and white-tailed deer ovarian tissue. Furthermore, the frozen-thawed equine and white-tailed deer ovarian tissue can be cultured for up to seven days.