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1

Nikolova-Damyanova, Boryana, and Nedjalka Handjieva. "Quantitative Determination of Swertiamarin and Gentiopicroside in Centaurium erythrea andC. turcicum by Densitometry." Phytochemical Analysis 7, no. 3 (May 1996): 140–42. http://dx.doi.org/10.1002/(sici)1099-1565(199605)7:3<140::aid-pca297>3.0.co;2-0.

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2

Stefkov, Gjoshe, Biljana Miova, Suzana Dinevska-Kjovkarovska, Jasmina Petreska Stanoeva, Marina Stefova, Gordana Petrusevska, and Svetlana Kulevanova. "Chemical characterization of Centaurium erythrea L. and its effects on carbohydrate and lipid metabolism in experimental diabetes." Journal of Ethnopharmacology 152, no. 1 (February 2014): 71–77. http://dx.doi.org/10.1016/j.jep.2013.11.047.

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3

Sefi, Mediha, Hamadi Fetoui, Nadya Lachkar, Adil Tahraoui, Badiaa Lyoussi, Tahia Boudawara, and Najiba Zeghal. "Centaurium erythrea (Gentianaceae) leaf extract alleviates streptozotocin-induced oxidative stress and β-cell damage in rat pancreas." Journal of Ethnopharmacology 135, no. 2 (May 2011): 243–50. http://dx.doi.org/10.1016/j.jep.2011.02.029.

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4

Budniak, Liliia, Liudmyla Slobodianiuk, Svitlana Marchyshyn, Olha Demydiak, and Iryna Dakhym. "Determination of amino acids of some plants from Gentianaceae family." Pharmacia 68, no. 2 (May 20, 2021): 441–48. http://dx.doi.org/10.3897/pharmacia.68.e67052.

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Medicines from plants are widely used in the complex treatment of different diseases every day. Therefore, theoretical and practical interest is the in-depth study of the herb of perspective plants like Centaurium erythraea Rafn. and Gentiana cruciata L. These plants have a long history of usage and interest of people. The aim of the study was to determine the content of amino acids in these plants. The amino acids composition and content in the herb of study species of the family Gentianaceae determined by the HPLC method. The results of the research revealed that the raw material of Centaurium erythraea Rafn. and Gentiana cruciata L. contains free and bound amino acids. Sixteen free and seventeen bound amino acids were identified in the herb of Centaurium erythraea Rafn. The herb of Gentiana cruciata L. contained thirteen free and fifteen bound amino acids. L-glutamic acid, L-arginine, L-aspartic acid, and L-cystine were predominant of Centaurium erythraea Rafn. herb. Amino acids L-lysine, L-serine, L-aspartic acid, and L-phenylalanine were present in the herb of Gentiana cruciata L. in the greatest amount. The metabolic processes in which these amino acids are involved connected to the medicinal properties of the study plants according to their use in official or nontraditional medicine.
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5

Liliya, Stoiko, Dakhym Iryna, Pokotylo Olena, and Marchyshyn Svitlana. "POLYSACCHARIDES IN CENTAURIUM ERYTHRAEA RAFN." International Journal of Research in Ayurveda & Pharmacy 8, no. 2 (June 11, 2017): 252–55. http://dx.doi.org/10.7897/2277-4343.082122.

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6

Valentão, PatrÍCia, Filipe Areias, Joana Amaral, Paula Andrade, and Rosa Seabra. "Tetraoxygenated Xanthones from Centaurium erythraea." Natural Product Letters 14, no. 5 (September 2000): 319–23. http://dx.doi.org/10.1080/10575630008043763.

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7

Merghem, Mounira, and Saliha Dahamna. "Antioxidant Activity of Centaurium erythraea Extracts." Journal of Drug Delivery and Therapeutics 10, no. 2 (March 15, 2020): 171–74. http://dx.doi.org/10.22270/jddt.v10i2.3935.

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Phototherapy has known a great evolution all the world and some medicinal plants are importance remedies of some diseases. Centaurium erythraea is one of the medicinal plants widely used in this field. In this study, we have evaluated the total polyphenolic and the flavonoid contents of the methanolic (ME) and aqueous (AqE) extracts of the plant Centaurium erythraea as well as its antioxidant activity using the DPPH (2'2-diphenylpicrylhydrazyl), β-carotene /linoleic acid bleaching and reducing power assays. The total polyphenolic content of the methanolic extract was 35,45± 0,041 μg AGE/mg extract and flavonoids was 6,65 ± 0,060 μg QE/mg. The total polyphenolic content of the aqueous extract was 54,27 ± 0,023 μg AGE/mg extract and flavonoids was 3,275 ± 0,003 μg QE/mg. The results of the DPPH test showed a powerful antioxidant activity with a very similar IC50 for the methanolic (IC50=0.232 ± 0.002mg/ml) and aqueous (0.208 ± 0.002mg/ml) extracts. The inhibitory activity of the tow extracts in the β-carotene/linoleic acid assay was (86.781 ± 0.17%) for the methanolic extract and (77.816 ± 0.69%) for the aqueous extract. ME has the higher reducing power (IC50 = 0.35 ± 0.066 mg / ml) compared to the AqE (IC50 = 1.31 ± 0.047 mg / ml). Keywords: Centaurium erythraea, antioxidant activity, polyphenols, DPPH scavenging, β-carotene, reducing power.
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8

Kaouadji, Mourad, Isabelle Vaillant, and Anne-Marie Mariotte. "Polyoxygenated Xanthones from Centaurium erythraea Roots." Journal of Natural Products 49, no. 2 (March 1986): 359. http://dx.doi.org/10.1021/np50044a037.

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9

Kumarasamy, Y., L. Nahar, P. J. Cox, M. Jaspars, and S. D. Sarker. "Bioactivity of secoiridoid glycosides from Centaurium erythraea." Phytomedicine 10, no. 4 (January 2003): 344–47. http://dx.doi.org/10.1078/094471103322004857.

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10

Bogdanović, Milica D., Katarina B. Ćuković, Angelina R. Subotić, Milan B. Dragićević, Ana D. Simonović, Biljana K. Filipović, and Slađana I. Todorović. "Secondary Somatic Embryogenesis in Centaurium erythraea Rafn." Plants 10, no. 2 (January 21, 2021): 199. http://dx.doi.org/10.3390/plants10020199.

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Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (se). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when se are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in Centaurium erythraea by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU). A medium containing 0.1 mgL−1 2,4-D and 0.25 mgL−1 CPPU was optimal in terms of the number of primary SE explants forming se, the number of well-developed se per explant, and morphological appearance of the obtained se. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL−1 2,4-D and 0.5 mgL−1 CPPU, only two cycles were achieved. Histological analysis revealed that secondary se are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology.
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11

Rich, T. C. G., A. McVeigh, and C. A. Stace. "NEW TAXA AND NEW COMBINATIONS IN THE BRITISH FLORA." Edinburgh Journal of Botany 76, no. 2 (December 3, 2018): 173–80. http://dx.doi.org/10.1017/s0960428618000288.

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Four new taxa and nine new combinations are required for forthcoming floristic works by the authors. The new taxa are Centaurium tenuiflorum (Hoffmanns. & Link) Fritsch subsp. anglicum T.C.G.Rich & McVeigh, subsp. nov.; Centaurium × klattii P.Fourn. ex T.C.G.Rich, hyb. nov.; Centaurium × ubsdellii T.C.G.Rich, hyb. nov.; and Gentianella amarella (L.) Börner subsp. occidentalis T.C.G.Rich & McVeigh, subsp. nov. The new combinations are Centaurium erythraea Rafn var. latifolium (Sm.) T.C.G.Rich, comb. et stat. nov.; Gentianella amarella (L.) Börner subsp. anglica (Pugsley) T.C.G.Rich & McVeigh, comb. et stat. nov.; Aria parviloba (T.C.G.Rich) Sennikov & Kurtto, comb. nov.; Cotula sessilis (Ruiz & Pav.) Stace, comb. nov.; Elymus × drucei (Stace) Stace, comb. nov.; Elymus repens (L.) Gould f. aristatus (Schumach.) Stace, comb. nov.; Elymus athericus (Link) Kerguélen f. setigerus (Dumort.) Stace, comb. nov.; Ulmus minor Mill. subsp. cornubiensis (Weston) Stace, comb. et stat. nov.; and Dysphania ambrosioides (L.) Mosyakin & Clemants var. anthelmintica (L.) Stace, comb. nov.
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12

Sandru, Daniela, Violeta Niculescu, Ecaterina Lengyel, and Ovidiu Tița. "Identification and Quantification of Total Polyphenols in Plants with Bioactive Potentially." International Journal of Pharmacology, Phytochemistry and Ethnomedicine 4 (August 2016): 47–51. http://dx.doi.org/10.18052/www.scipress.com/ijppe.4.47.

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This meaning of this specific work is to identify and quantify the polyphenolic compounds that exist in plants with bioactive potentially. The study was monitorising 16 different plants: bilberry (Vaccinium myrtillus), artichoke (Cynara scolymus), chicory (Cichorium intybus), dumb (Teucrium chamaedrys), fennel (Foeniculum vulgare), thorn (Xanthium spinosum), juniper (Juniperus communis), mint (Mentha), cranberry (Vaccinium vitis-idaea), hawthorn (Crataegus monogyna), wormwood (Artemisia absinthium), willow herb (Epilobium), lemon balm (Melissa officinalis), St. John's wort (Hypericum perforatum), oregano (Origanum vulgare), centaury (Centaurium erythraea). The total polyphenolic compound was determined onspectrophotometricmethod,Folin-Ciocalteu. The polyphenols have a very wide range value starting on low amounts on centaury (Centaurium erythraea) 271.613 mg/L and reaching highest values of 5975.616 mg/L in wormwood (Artemisia absinthium). The results can be use in the design of digestive drinks in the food industry due to higher concentration of total polyphenols in the studied plants.
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13

Șandru, Daniela Maria, Diana Stegăruș, Ecaterina Lengyel, and Ovidiu Tița. "Identification and Quantification the Aroma Compounds from Autochthonous Plants with GC/FID System." Management of Sustainable Development 8, no. 2 (December 1, 2016): 25–28. http://dx.doi.org/10.1515/msd-2017-0004.

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Abstract The aims of this paper is to identify and quantify the flavor compounds that exist in plants in order to valorification in the nutritional supplements and food industry. The study was monitorising 16 different plants and the specific aromas was determined on the GC/FID system. The content esters can be reached on intervals starting from 1.0086 mg/L in Juniperus communis to 27.0172 mg/L in Teucrium chamaedrys. The superior alcohols values parameters start from 5.1209 mg/L for Melissa officinalis to 132.0837 mg/L in Teucrium chamaedrys. The aldehydes started from at minimum of 1.0218 mg/L at Centaurium erythraea and a maximum of 37.1829 at Vaccinium vitis-idaea. The terpene compounds starts from 0.0182 mg/L for Centaurium erythraea to 19.9280 mg/L at Teucrium chamaedrys. The results we obtained by this study represents the foundation for us in these bio potentially active plants in two important industries: the pharmaceutical and the food one-by obtaining nutritional supplements, liquors, extracts and tinctures.
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14

Banjanac, Tijana, Marijana Skoric, Mario Belamaric, Jasmina Nestorovic-Zivkovic, Danijela Misic, Mihailo Jelic, Slavica Dmitrovic, and Branislav Siler. "Pursuit for est microsatellites in a tetraploid model from de novo transcriptome sequencing." Genetika 50, no. 2 (2018): 687–703. http://dx.doi.org/10.2298/gensr1802687b.

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Available scientific literature reports very few microsatellite markers derived from tetraploid genomes using de novo transcriptome sequencing, mostly because their gain usually represents a major computational challenge due to complicated combinatorics during assembly of sequence reads. Here we present a novel approach for mining polymorphic microsatellite loci from transcriptome data in a tetraploid species with no reference genome available. Pairs of 114 bp long de novo sequenced transcriptome reads of Centaurium erythraea were merged into short contigs of 170-200 bp each. High accuracy assembly of the pairs of reads was accomplished by a minimum of 14 bp overlap. Sequential bioinformatics operations involved fully free and open-source software and were performed using an average personal computer. Out of the 13 150 candidate contigs harboring SSR motifs obtained in a final output, we randomly chose 16 putative markers for which we designed primers. We tested the effectiveness of the established bioinformatics approach by amplifying them in eight different taxa within the genus Centaurium having various ploidy levels (diploids, tetraploids and hexaploids). Nine markers displayed polymorphism and/or transferability among studied taxa. They provided 54 alleles in total, ranging from 2 to 14 alleles per locus. The highest number of alleles was observed in C. erythraea, C. littorale and a hybridogenic taxon C. pannonicum. The developed markers are qualified to be used in genetic population studies on declining natural populations of Centaurium species, thus providing valuable information to evolutionary and conservation biologists. The developed cost-effective methodology provides abundant de novo assembled short contigs and holds great promise to mine numerous additional EST-SSR-containing markers for possible use in genetics population studies of tetraploid taxa within the genus Centaurium.
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15

Beerhues, Ludger. "Benzophenone synthase from cultured cells of Centaurium erythraea." FEBS Letters 383, no. 3 (April 1, 1996): 264–66. http://dx.doi.org/10.1016/0014-5793(96)00265-7.

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16

Paunović, Danijela M., Katarina B. Ćuković, Milica D. Bogdanović, Slađana I. Todorović, Milana M. Trifunović-Momčilov, Angelina R. Subotić, Ana D. Simonović, and Milan B. Dragićević. "The Arabinogalactan Protein Family of Centaurium erythraea Rafn." Plants 10, no. 9 (September 9, 2021): 1870. http://dx.doi.org/10.3390/plants10091870.

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Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.
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17

Beerhues, L. "CONSTITUTIVE AND ELICITOR-INDUCED XANTHONES IN CELL CULTURES OF CENTAURIUM ERYTHRAEA AND CENTAURIUM LITTORALE." Acta Horticulturae, no. 381 (December 1994): 250–57. http://dx.doi.org/10.17660/actahortic.1994.381.30.

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18

Bakour, Meryem, Hassan Laaroussi, Driss Ousaaid, Bouchra Oumokhtar, and Badiaa Lyoussi. "Antioxidant and Antibacterial Effects of Pollen Extracts on Human Multidrug-Resistant Pathogenic Bacteria." Journal of Food Quality 2021 (June 30, 2021): 1–11. http://dx.doi.org/10.1155/2021/5560182.

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The present work was designed to search the possible antibacterial effect of the ethanolic extract of pollens from six botanical origins, Punica granatum, Quercus ilex, Centaurium erythraea, Coriandrum sativum, Ruta graveolens, and Citrus aurantium, against multidrug pathogenic bacteria, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Acinetobacter baumannii, Klebsiella pneumoniae, and Staphylococcus aureus. The content of phenolic compounds, flavones, and flavonols was measured. The antioxidant activities were evaluated using four assays: total antioxidant capacity, DPPH, ABTS, and reducing power. Antibacterial activity was studied using the agar disk diffusion method, and the MIC and MBC were determined. Results obtained showed a positive correlation between the antioxidant content of pollen extracts and the antibacterial capacity, Punica granatum and Quercus ilex pollen extracts were the most efficient against Gram-positive and Gram-negative bacteria, and Centaurium erythraea, Coriandrum sativum, and Ruta graveolens had a moderate effect, while Citrus aurantium had no antibacterial effect. It is concluded that pollens can be a good source of bioactive molecules that exhibit potent antioxidant effects and strong antibacterial activities.
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Haloui, Mounsif, Liliane Louedec, Jean-Baptiste Michel, and Badiaa Lyoussi. "Experimental diuretic effects of Rosmarinus officinalis and Centaurium erythraea." Journal of Ethnopharmacology 71, no. 3 (August 2000): 465–72. http://dx.doi.org/10.1016/s0378-8741(00)00184-7.

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20

Chda, Alae, Mohamed El Kabbaoui, Paula Fresco, Dany Silva, Jorge Gonçalves, Andreia P. Oliveira, Paula B. Andrade, et al. "Centaurium Erythraea Extracts Exert Vascular Effects through Endothelium- and Fibroblast-dependent Pathways." Planta Medica 86, no. 02 (October 23, 2019): 121–31. http://dx.doi.org/10.1055/a-1023-8918.

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Abstract Centaurium erythraea is a plant used in traditional medicine for several cardiovascular disorders, namely hypertension, but there is no scientific evidence able to provide a molecular basis for its claimed antihypertensive effects. After a preliminary screen of extracts obtained from sequential extraction of C. erythraea aerial parts, effects of the methanolic fraction (MFCE) on changes in perfusion pressure of isolated rat mesenteric vascular bed (MVB) and in rat cardiac fibroblasts proliferation were investigated, gathering information on the mechanisms involved in endothelium-dependent effects and their dependence on a pro-proliferative stimulus. The HPLC-DAD determination of the phenolics content of MFCE revealed the presence of 22 phenolic compounds. MFCE reduced (63.3 ± 3.9%; n = 4) perfusion pressure in MVB and almost completely abrogated the Ang II-induced increase in cardiac fibroblasts proliferation. Reduction of the perfusion pressure caused by MFCE was endothelium-dependent and occurred in parallel with an increase in NO release. These effects were inhibited by muscarinic receptor antagonists, by L-NAME (a NO synthase inhibitor), and by ODQ (a soluble guanylate cyclase inhibitor). Experiments revealed that effects required the involvement of K+ channels, being inhibited by tetraethylamonium (TEA; a Ca2+ activated K+ channels inhibitor) and by glibenclamide (an ATP-sensitive K+ channels inhibitor). In conclusion, extracts from C. erythraea, particularly the compounds present in the MFCE, induce endothelium-dependent vasodilation and prevent fibroblast proliferation induced by angiotensin II, which can account for the claimed antihypertensive effects of C. erythraea in traditional medicine.
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21

Guedes, Laura, Pedro B. P. S. Reis, Miguel Machuqueiro, Asma Ressaissi, Rita Pacheco, and Maria Luísa Serralheiro. "Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies." Molecules 24, no. 20 (October 22, 2019): 3795. http://dx.doi.org/10.3390/molecules24203795.

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Centaurium erythraea is recommended for the treatment of gastrointestinal disorders and to reduce hypercholesterolemia in ethno-medicinal practice. To perform a top-down study that could give some insight into the molecular basis of these bioactivities, decoctions from C. erythraea leaves were prepared and the compounds were identified by liquid chromatography-high resolution tandem mass spectrometry (LC–MS/MS). Secoiridoids glycosides, like gentiopicroside and sweroside, and several xanthones, such as di-hydroxy-dimethoxyxanthone, were identified. Following some of the bioactivities previously ascribed to C. erythraea, we have studied its antioxidant capacity and the ability to inhibit acetylcholinesterase (AChE) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR). Significant antioxidant activities were observed, following three assays: free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) reduction; lipoperoxidation; and NO radical scavenging capacity. The AChE and HMGR inhibitory activities for the decoction were also measured (56% at 500 μg/mL and 48% at 10 μg/mL, respectively). Molecular docking studies indicated that xanthones are better AChE inhibitors than gentiopicroside, while this compound exhibits a better shape complementarity with the HMGR active site than xanthones. To the extent of our knowledge, this is the first report on AChE and HMGR activities by C. erythraea decoctions, in a top-down analysis, complemented with in silico molecular docking, which aims to understand, at the molecular level, some of the biological effects ascribed to infusions from this plant.
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22

Kumarasamy, Y., L. Nahar, and S. D. Sarker. "Bioactivity of gentiopicroside from the aerial parts of Centaurium erythraea." Fitoterapia 74, no. 1-2 (February 2003): 151–54. http://dx.doi.org/10.1016/s0367-326x(02)00319-2.

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23

Pataczek, Lisa, Antigoni Cheilari, Sabine Zikeli, Sonja Sturm, Hermann Stuppner, and Sabine Gruber. "Centaurium erythraea Cultivation Method for Optimal Yield and Product Quality." Journal of Herbs, Spices & Medicinal Plants 23, no. 3 (March 15, 2017): 193–215. http://dx.doi.org/10.1080/10496475.2017.1295414.

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24

STOIKO, Liliya, and Khrystyna KURYLO. "Development of optimal technology of alcohol extract centaurium erythraea rafn. herb." Archives of the Balkan Medical Union 53, no. 4 (December 7, 2018): 523–28. http://dx.doi.org/10.31688/abmu.2018.53.4.06.

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25

El Menyiy, Naoual, Fatima-Ezzahrae Guaouguaou, Aicha El Baaboua, Nasreddine El Omari, Douae Taha, Najoua Salhi, Mohammad Ali Shariati, et al. "Phytochemical properties, biological activities and medicinal use of Centaurium erythraea Rafn." Journal of Ethnopharmacology 276 (August 2021): 114171. http://dx.doi.org/10.1016/j.jep.2021.114171.

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Gubar, Svitlana, Anna Materiienko, Liudas Ivanauskas, Volodymyr Mishchenko, Olha Vasylieva, and Victoriya Georgiyants. "A novel comparative chromatographic research of secoiridoid glycosides in two species of centaury herb." ScienceRise: Pharmaceutical Science, no. 3(31) (June 30, 2021): 28–33. http://dx.doi.org/10.15587/2519-4852.2021.235774.

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The aim: A novel comparative analysis of the secoiridoid glycosides composition in Centaurium erythraea Rafn. and Cеntaurium pulchellum (Sw.) Druce has being described. Swertiamarin has been chosen as an active marker and its comparative quantitative estimation in two species of the raw material has been carried out by HPLC method. Materials and methods: The quantitative HPLC analysis of swertiamarin was conducted using a chromatographic column ACE 5 C18. Methanol and 0,5 % acetic acid aqueous solution were used as mobile phases; the chromatographic procedure was carried out in a gradient mode. Results: The content of swertiamarin in the C. pulchellum herb ranges from 2.51 to 3.07 mg/100 mg. In that time swertiamarin content in C. erythraea herb varies and depends from 3.83 to 8.94 mg/100 mg. The content of swertiamarin in C. pulchellum herb is much lower than in C. erythraea herb. Taking into account this fact the possibility of using of C. pulchellum herb for medicine instead of C. erythraea herb must be proven additionally by biological experiments. Also it could be preliminary concluded that the most appropriate climate for swertiamarin accumulation in C. erythraea herb is located in Central and Eastern parts of Ukraine. Conclusions: It was discovered that the main representative of secoiridoid glycosides in C. erythraea is swertiamarin when in C. pulchellum Druce that are represented by both sweroside and swertiamarin. A more perspective raw material – C. erythraea herb, according to the content of swertiamarin, was chosen
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27

Banjanac, Tijana, Sanja Đurović, Mihailo Jelić, Milan Dragićević, Danijela Mišić, Marijana Skorić, Jasmina Nestorović Živković, and Branislav Šiler. "Phenotypic and Genetic Variation of an Interspecific Centaurium Hybrid (Gentianaceae) and Its Parental Species." Plants 8, no. 7 (July 14, 2019): 224. http://dx.doi.org/10.3390/plants8070224.

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Interspecific hybridization is one of the major actuators of evolutionary changes in plants. As the result of allopolyploid hybridization, offspring may gain different ploidy levels in comparison to parental species, which can provide them instant reproductive isolation. Two tetraploid sister species, Centaurium erythraea and C. littorale, readily cross-fertilize, resulting in hybrids of various ploidy. In northern Serbia, two stable populations of a hexaploid taxon C. pannonicum have been documented. It has been proposed previously that this taxon emerged after an interspecific hybridization event between two tetraploid sister-species: C. erythraea and C. littorale subsp. compressum. The existing populations of the hybridogenic taxon, as well as neighboring populations of the two parental taxa were here characterized by both morphometrics and molecular markers (EST-SSR and trnL-F). Three leaf and two flower characteristics were found to be informative in delimitation of the parental taxa and in their discernment from hybrid individuals, the latter having intermediate values. Eight microsatellite markers were found to have good ability to distinguish studied taxa, placing C. pannonicum in closer relationship with C. erythraea. Conversely, trnL-F plastid marker nominated C. littorale subsp. compressum to be the donor of the C. pannonicum plastid DNA. Reproductive isolation of the hexaploid hybrid individuals from the parental species should be examined as the next logical step in describing the new species.
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28

Lavado Morales, Ivar Jines, Diana Esmeralda Andamayo Flores, Diana Esmeralda Castillo Andamayo, and Vilma Amparo Junchaya Yllescas. "Evaluación preliminar de 10 plantas medicinales del Valle del Mantaro mediante el método cualitativo (fitoquímico) para uso farmacéutico." Visionarios en ciencia y tecnología 6, no. 1 (September 10, 2021): 38–48. http://dx.doi.org/10.47186/visct.v6i1.88.

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Objetivo: Determinar preliminarmente 10 plantas medicinales del Valle del Mantaro mediante el método cualitativo (fitoquímico) para uso farmacéutico. Material y Métodos: Cualitativo (fitoquímico) para uso farmacéutico. Resultados: La actividad farmacognósicas de las plantas medicinales son utilizadas en el Valle del Mantaro: Xanthium spinosum (Juan alonso) como antitumoral y anticancerígena, Equisetum arvense (Cola de caballo) como osteoporosis, coagulación sanguínea Mentha sativa L (Hierba buena) para el nerviosismo, Rumex crispus L. (Cuturrumasa) como antiinflamatorio, Piper aduncum (Matico) como antiviral, Centauriun erythraea Rafn. (Canchalagua) como antitumorales, antidiabéticas, Schinus molle (Molle) como antirreumático, Psoralea glandulosaxonomía (Culen) como hipoglucemiente, Minthostachys mollis (Inca muña) como antireumático y acción carminativa, Taraxacum officinale (Diente de león) como hipoglucemiante. Conclusiones: De acuerdo al análisis fitoquímico de las plantas, confirman la actividad farmacológica para el uso medicinal: Xanthium spinosum (Juan Alonso), Equisetum arvense (Cola de caballo), Mentha sativa L. (Hierba buena), Rumex crispus L. (Cuturrumasa), Piper aduncum (Matico), Centauriun erythraea Rafn (Canchalagua), Schinus molle (Molle), Psoralea glandulosaxonomía (Culen), Minthostachys mollis (Inca Muña) y Taraxacum officinale (Diente de León). El uso de las plantas medicinales en el Valle del Mantaro por sus metabolitos secundarios pueden asociarse a prometedoras actividades medicinales como: antitumorales, anticancerígenos, osteoporosis, antiviral, antidiabéticas, antirreumático; siendo candidatos para estudios con mayor profundidad fitoquímica y farmacobiólogica.
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Siler, B., Danijela Misic, Biljana Filipovic, Zorica Popovic, Tijana Cvetic, and A. Mijovic. "Effects of salinity on in vitro growth and photosynthesis of common centaury (Centaurium erythraea Rafn.)." Archives of Biological Sciences 59, no. 2 (2007): 129–34. http://dx.doi.org/10.2298/abs0702129s.

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The effects of salinity on in vitro growth, morphogenesis, photosynthetic rate (Pn), PSII efficiency (Fv/Fm), and chlorophyll content were investigated in Centaurium erythraea Rafn. Root growth was more adversely affected by increasing NaCl concentration than was shoot growth. High salt concentrations were effective in induction of axillary and adventitious buds on shoots, with 400 mM NaCl being the most efficient one. Values of Pn and Fv/Fm increased at moderate salt levels, but decreased when 400 mM NaCl was applied. Chlorophyll a and b contents and total chlorophyll had a decreasing trend with increasing supply of NaCl in the growth medium. .
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30

Bouyahya, Abdelhakim, Youssef Bakri, Omar Belmehdi, Abdeslam Et-Touys, Jamal Abrini, and Nadia Dakka. "Phenolic extracts of Centaurium erythraea with novel antiradical, antibacterial and antileishmanial activities." Asian Pacific Journal of Tropical Disease 7, no. 7 (July 7, 2017): 433–39. http://dx.doi.org/10.12980/apjtd.7.2017d6-462.

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31

Simonović, Ana D., Milana M. Trifunović-Momčilov, Biljana K. Filipović, Marija P. Marković, Milica D. Bogdanović, and Angelina R. Subotić. "Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review." Plants 10, no. 1 (December 31, 2020): 70. http://dx.doi.org/10.3390/plants10010070.

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Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.
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32

Božunović, Jelena, Marijana Skorić, Dragana Matekalo, Suzana Živković, Milan Dragićević, Neda Aničić, Biljana Filipović, Tijana Banjanac, Branislav Šiler, and Danijela Mišić. "Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves." Plants 8, no. 12 (December 11, 2019): 589. http://dx.doi.org/10.3390/plants8120589.

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Centaurium erythraea Rafn produces and accumulates various biologically active specialized metabolites, including secoiridoid glucosides (SGs), which help plants to cope with unfavorable environmental conditions. Specialized metabolism is commonly modulated in a way to increase the level of protective metabolites, such as SGs. Here, we report the molecular background of the wounding-induced changes in SGs metabolism for the first time. The mechanical wounding of leaves leads to a coordinated up-regulation of SGs biosynthetic genes and corresponding JA-related transcription factors (TFs) after 24 h, which results in the increase of metabolic flux through the biosynthetic pathway and, finally, leads to the elevated accumulation of SGs 96 h upon injury. The most pronounced increase in relative expression was detected for secologanin synthase (CeSLS), highlighting this enzyme as an important point for the regulation of biosynthetic flux through the SG pathway. A similar expression pattern was observed for CeBIS1, imposing itself as the TF that is prominently involved in wound-induced regulation of SGs biosynthesis genes. The high degree of positive correlations between and among the biosynthetic genes and targeted TFs expressions indicate the transcriptional regulation of SGs biosynthesis in response to wounding with a significant role of CeBIS1, which is a known component of the jasmonic acid (JA) signaling pathway.
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33

Trifunović-Momčilov, Milana, Václav Motyka, Ivana Č. Dragićević, Marija Petrić, Slađana Jevremović, Jiří Malbeck, Josef Holík, Petre I. Dobrev, and Angelina Subotić. "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro." Journal of Plant Growth Regulation 35, no. 2 (November 20, 2015): 543–52. http://dx.doi.org/10.1007/s00344-015-9558-x.

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34

Zivkovic, S., M. Devic, B. Filipovic, Z. Giba, and D. Grubisic. "Effect of NaCl on seed germination in some Centaurium Hill. Species (Gentianaceae)." Archives of Biological Sciences 59, no. 3 (2007): 227–31. http://dx.doi.org/10.2298/abs0703227z.

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The influence of high NaCl concentrations on seed germination in both light and darkness was examined in the species Centaurium pulchellum, C. erythraea, C. littorale, C. spicatum, and C. tenuiflorum. Salt tolerance was found to depend on the life history of the seeds. To be specific, seeds of all five species failed to complete germination when exposed to continuous white light if kept all the time in the presence of 100-200 mM and greater NaCl concentrations. However, when after two weeks NaCl was rinsed from the seeds and the seeds were left in distilled water under white light for an additional two weeks, all species completed germination to a certain extent. The percent of germination not only depended on NaCl concentration in the prior medium, but was also species specific. Thus, seeds of C. pulchellum, C. erythraea, and C. littorale completed germination well almost irrespective of the salt concentration previously experienced. On the other hand, seeds of C. tenuiflorum completed germination poorly if NaCl concentrations in the prior media were greater than 200 mM. When seeds after washing were transferred to darkness for an additional 14 days, they failed to complete germination if previously imbibed on media containing NaCl concentrations greater than 400 mM. However, the seeds of all species, even if previously imbibed at 800 mM NaCl, could be induced to complete germination in darkness by 1 mM gibberellic acid. .
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35

Barillas, Wagner, and Ludger Beerhues. "3-Hydroxybenzoate:Coenzyme A Ligase from Cell Cultures of Centaurium erythraea: Isolation and Characterization." Biological Chemistry 381, no. 2 (February 15, 2000): 155–60. http://dx.doi.org/10.1515/bc.2000.021.

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Abstract In xanthone biosynthesis, 3-hydroxybenzoate:coenzyme A ligase (3HBL) supplies the starter substrate for the formation of an intermediate benzophenone. 3HBL from cell cultures of the medicinal plant Centaurium erythraea was purified to apparent homogeneity using a sevenstepprocedure. The enzyme was an AMPforming CoA ligase with a K = 14.7 for 3-hydroxybenzoic acid, 8.5 for coenzyme A and 229 for ATP. The pH and temperature optima were 7.5 and 35C, respectively. In SDSPAGE, two polypeptides of M 41500 and 40500 were detected. Both proteins were structurally related to each other as shown by tryptic digestion. Their Ntermini were blocked. The difference in their apparent molecular masses could not be attributed to glycosylation. 3HBL had a native M of approx. 50000 and is thus active as a monomer.
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36

Skrajna, Teresa, and Helena Kubicka. "Segetal flora of the Wigry National Park." Acta Agrobotanica 64, no. 3 (2012): 119–32. http://dx.doi.org/10.5586/aa.2011.039.

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Segetal flora of the Wigry National Park (Poland) was studied in the period 2008-2010 within an area occupied by 33 villages. The analysis was based on 195 phytosociological relevés taken in fields of cereal crops, root plants and in stubble fields. The total number of species recorded in agrocenoses was 181 and they represented 36 botanical families. The most numerous families were Asteraceae, Fabaceae and Brasicaceae. The segetal flora of the Wigry National Park included mostly native species of apophytes (53.6%) that predominated over anthropophytes. Among apophytes, meadow apophytes (35 species) and those from waterside habitats (26 species) were noted most often, archaeophytes (74 species) were predominant among anthropophytes, while epecophytes (9 species) and ergasiophytes (1 species) were much less abundant. Among the life forms analysed, therophytes (61.9%) predominated over hemicryptophytes (27.1%) and geophytes (11%). The number of short-lived species in the flora of this Park was much greater (68.5%) than that of perennial ones (31.5%). The flora of the Park included 31 rare species classified in different categories of threat. Among them, <i>Centaurium pulchellum</i> and <i>Centaurium erythraea</i> belong to fully protected species (Dz.U. (Polish Journal of Laws) 2004 No. 168, item 1764).
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37

Mijajlovic, Nada, Dragoljub Grubisic, Zlatko Giba, and Radomir Konjevic. "The effect of plant growth regulators on centaury (Centaurium erythraea Rafn) seeds germination." Archives of Biological Sciences 57, no. 1 (2005): 25–28. http://dx.doi.org/10.2298/abs0501025m.

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Centaury seeds are light-requiring. Long-term red light irradiation caused more than 80% of seeds to germinate. Seeds did not germinate in darkness. Gibberellic acid and GA7 can replace light, but N-substituted phtalimide AC 94,377 was ineffective. Light-induced germination was inhibited by abscisic acid and growth retardants such as ancymidol, tetcyclacis, and paclobutrazole. Growth retardant-caused inhibition can be overcome by the addition of gibberellic acid.
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38

Valentão, Patrícia, Paula B. Andrade, Eurico Silva, Ana Vicente, Henrique Santos, M. Lourdes Bastos, and Rosa M. Seabra. "Methoxylated Xanthones in the Quality Control of Small Centaury (Centaurium erythraea) Flowering Tops." Journal of Agricultural and Food Chemistry 50, no. 3 (January 2002): 460–63. http://dx.doi.org/10.1021/jf0109571.

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39

Schmidt, Werner, Stefan Peters, and Ludger Beerhues. "Xanthone 6-hydroxylase from cell cultures of Centaurium erythraea RAFN and Hypericum androsaemum L." Phytochemistry 53, no. 4 (February 2000): 427–31. http://dx.doi.org/10.1016/s0031-9422(99)00566-x.

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40

Piątczak, Ewelina, and Halina Wysokińska. "In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants." Acta Societatis Botanicorum Poloniae 72, no. 4 (2011): 283–88. http://dx.doi.org/10.5586/asbp.2003.036.

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Various explants from 30-day-old seedlings of <em>Centaurium erythraea </em>Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ± 2.2 from a single shoot tip) was obtained on Murashige and Skoog medium (MS) supplemented with indole-3-acetic acid (IAA) (0.57 μM) and 6-benzylaminopurine (BAP) (4.4 μM). Adventitious shoot regeneration was also achieved through organogenesis from calluses obtained from hypocotyls, cotyledons, roots and leaves on MS medium containing IAA (2.85 μM) and BAP (0.88 μM). Significant differences were noted between explant types in their effects on shoot regeneration. In the primary culture, the best response was obtained either from calluses derived from roots or leaves (44.4 ± 4.5 and 40.2 ± 6.0 shoots per callus, respectively). The number of subcultures of inoculated calluses affected both the multiplication rate (the number of shoots/explant) and shoot morphology (the frequency of shoot hyperhydricity). Shoots rooted with the frequency of 94-100% after culture on MS medium without growth regulators. Plantlets were successfully acclimatized (97%) under high relative humidity and then moved to the greenhouse.
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41

Piatczak, Ewelina, Aleksander Chmiel, and Halina Wysokinska. "Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids." Biotechnology Letters 27, no. 10 (May 2005): 721–24. http://dx.doi.org/10.1007/s10529-005-5189-9.

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42

Piatczak, Ewelina, Aleksandra Krolicka, and Halina Wysokinska. "Genetic transformation of Centaurium erythraea Rafn by Agrobacterium rhizogenes and the production of secoiridoids." Plant Cell Reports 25, no. 12 (July 14, 2006): 1308–15. http://dx.doi.org/10.1007/s00299-006-0155-0.

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43

Brudzińska-Kosior, Anna, Grzegorz Kosior, Aleksandra Samecka-Cymerman, Krzysztof Kolon, Lucyna Mróz, and Alexander J. Kempers. "Metal contents in Centaurium erythraea and its biometry at various levels of environmental pollution." Ecotoxicology and Environmental Safety 80 (June 2012): 349–54. http://dx.doi.org/10.1016/j.ecoenv.2012.04.005.

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44

Boudjelthia, W. Kouadri, K. Hammadi, M. Kouidri, A. Noui, and N. Djebli. "Ethnobotanical survey of an -diabe c plants applied in West of Algeria." South Asian Journal of Experimental Biology 8, no. 2 (January 14, 2019): 57–62. http://dx.doi.org/10.38150/sajeb.8(2).p57-62.

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This study was carried out in order to set up an inventory list of medicinal plants used in traditional medicine to treat diabetes for which an ethnobotanical test was carried out on 670 diabe c subjects in the Western region of Algeria (Chlef, Mostaganem, Mascara, Oran, Sidi-bel-Abbes, Saida and Tiaret) using a survey. 24 an hyperglycemic medicinal plants were listed of which Berberis vulgaris, Zygophyllum geslini, Erythraea centaurium and Olea eu- ropea represented a broad spectrum of use. Likewise, the an hyperglycemic recipes were prepared mainly in decoc on (45.37%) and infusion (39.62%), and from leaves (42%) and aerial parts (16%). A very valuable data base has been reported for further research, especially for some elds such as phytochemistry and phytopharmacology that aim to identify new natural principles.
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45

Subotic, A., S. Budimir, D. Grubisic, and I. Momcilovic. "Direct Regeneration of Shoots from Hairy Root Cultures of Centaurium erythraea Inoculated with Agrobacterium rhizogenes." Biologia plantarum 46, no. 4 (December 1, 2003): 617–19. http://dx.doi.org/10.1023/b:biop.0000041074.81033.3a.

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46

Trifunović, Milana, Aleksandar Cingel, Ana Simonović, Slađana Jevremović, Marija Petrić, Ivana Č. Dragićević, Václav Motyka, Petre I. Dobrev, Lenka Zahajská, and Angelina Subotić. "Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn." Plant Cell, Tissue and Organ Culture (PCTOC) 115, no. 2 (June 28, 2013): 139–50. http://dx.doi.org/10.1007/s11240-013-0347-6.

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47

Tahraoui, Adel, Zafar H. Israili, and Badiâa Lyoussi. "Acute and sub-chronic toxicity of a lyophilised aqueous extract of Centaurium erythraea in rodents." Journal of Ethnopharmacology 132, no. 1 (October 2010): 48–55. http://dx.doi.org/10.1016/j.jep.2010.07.038.

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48

Proskurova, Ya A., S. M. Gubar, and V. A. Georgiyants. "Qualitative composition determination of the biologically active compounds in raw material of Centaurium erythraea Rafn. and Centaurium pulchellum (Sw.) Druce herb by liquid chromatographymass spectrometry method." Upravlìnnâ, ekonomìka ta zabezpečennâ âkostì v farmacìï, no. 3(51) (September 15, 2017): 20–24. http://dx.doi.org/10.24959/uekj.17.27.

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49

Tuluce, Yasin, Halil Ozkol, Ismail Koyuncu, and Hatice Ine. "Gastroprotective effect of small centaury (Centaurium erythraeaL) on aspirin-induced gastric damage in rats." Toxicology and Industrial Health 27, no. 8 (March 17, 2011): 760–68. http://dx.doi.org/10.1177/0748233710397421.

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50

Simonovic, Ana, Milana Trifunovic, M. Raspor, A. Cingel, Milica Bogdanovic, M. Dragicevic, and Angelina Subotic. "Dimethyl sulfoxide improves sensitivity and specificity of RT-PCR and qRT-PCR amplification of low-expressed transgenes." Archives of Biological Sciences 64, no. 3 (2012): 865–76. http://dx.doi.org/10.2298/abs1203865s.

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The expression of transgenes in a host plant may be low for a number of reasons. Both low expression and poor specificity of amplification were encountered during analysis of the expression of the Arabidopsis cytokinin oxidase/dehydrogenase (AtCKX1) gene in transgenic Centaurium erythraea. The optimization of the PCR protocol involved a gradient of annealing temperatures, as well as the application of seven PCR enhancers: formamide, dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, trehalose, BSA and Tween-20. The best results for AtCKX1 amplification were obtained at 55.1?C, with the addition of 5% DMSO. Glycerol and trehalose also improved the sensitivity of amplification, while formamide, ethylene glycol and BSA enhanced only the amplification of control purified targets, but not the transcripts. Tween-20 inhibited PCR. DMSO enhanced AtCKX1 PCR amplification and improved the specificity of qPCR amplification, as well as the assay reproducibility. This work emphasizes the usefulness of additives, which are rarely used for PCR optimization in real-time experiments.
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