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Journal articles on the topic 'ESBL producers'

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Published: 5 June 2025

Last updated: 16 July 2025

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1

Sarwar1, Yasra, Samina Shaheen, Naila Mushtaq, and Abdul Haque. "Detection and Molecular Typing of Extended Spectrum Beta Lactamases (ESBLs) Producing Gram-Negative Bacteria from Wound Infections." Lahore Garrison University Journal of Life Sciences 1, no. 04 (2017): 236–47. http://dx.doi.org/10.54692/lgujls.2017.0104137.

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ABSTRACT: Extended spectrum β-lactamases (ESBLs) producing bacteria, which are mainly Gram-negative, have emerged as a major threat in recent years as they are resistant to most antimicrobials. This study was conducted to investigate the prevalence of ESBL producing Gram-negative bacteria in woundinfections and to map their antimicrobial resistance profile. A total of 100 samples were collected and identified from infected wounds. The Gram-negative bacteria were checked for identification as ESBL producers by using double disc diffusion test. The identified ESBL producers were subjected to PCR

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Rana Essa muslem, Rana H Raheema та Qasim Dawood Yasir. "Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq". World Journal of Biology Pharmacy and Health Sciences 12, № 2 (2022): 094–104. http://dx.doi.org/10.30574/wjbphs.2022.12.2.0178.

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Intensive care units (ICU) are epicenters for the emergence of antibiotic-resistant Gram negative bacteria and multi-resistant Gram positive infections, largely due to the inappropriate use of antimicrobials. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. The findings of the disc diffusion method used to test the isolates for ‎antibiotic susceptibility showed that Amikacin was the most effective ‎antibiotic against klebsiella pneumonia and pseudomonas aeruginosa.

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Poirel, Laurent, Javier Fernández та Patrice Nordmann. "Comparison of Three Biochemical Tests for Rapid Detection of Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae". Journal of Clinical Microbiology 54, № 2 (2015): 423–27. http://dx.doi.org/10.1128/jcm.01840-15.

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Enterobacterial isolates producing clavulanic-inhibited extended-spectrum β-lactamases (ESBLs) are increasingly spreading in the community and are often responsible for nosocomial infections. Rapid biochemical tests have been developed recently for their detection. Three tests, namely, the Rapid ESBL NDP test, the β-Lacta test, and the Rapid ESBL Screen, have been evaluated with a collection of 108 well-characterized strains, including wild-type strains, strains producing ESBLs, overexpressed cephalosporinases, and carbapenemases. The ESBL NDP test and the Rapid ESBL Screen (a copy of the ESBL

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Kannaiyan, Moorthy, Gedif Meseret Abebe, Chinnasamy Kanimozhi, et al. "PREVALENCE OF EXTENDED-SPECTRUM BETA-LACTAMASE PRODUCING ENTEROBACTERIACEAE MEMBERS ISOLATED FROM CLINICALLY SUSPECTED PATIENTS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 5 (2018): 364. http://dx.doi.org/10.22159/ajpcr.2018.v11i5.19363.

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Objective: Emergence of extended-spectrum beta-lactamases (ESBLs) production poses another clinical problem with Gram-negative bacterial infections. The present study was aimed to evaluate the ESBL producers among various clinical samples of clinically suspected patients.Methods: A total of 1279 samples (urine [918], pus [207] and stool [154]) were collected and 465 isolates (Escherichia coli [320], Enterobacter aerogenes [119] and Klebsiella pneumoniae [26]) were isolated and screened for the presence of ESBL producers using combination disc method and double disc synergy test.Results: Of the

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Tetteh, Francis Kwame Morgan, Anthony Ablordey, Noah Obeng-Nkrumah, and Japheth Awuletey Opintan. "Extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae recovered from patients at the Tamale Teaching Hospital, Ghana." PLOS ONE 19, no. 4 (2024): e0300596. http://dx.doi.org/10.1371/journal.pone.0300596.

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Introduction Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae are pathogens of significant public health interest for which new antibiotics are urgently needed. Aim To determine the prevalence of ESBLs in E. coli and K. pneumoniae isolates from patients attending the Tamale Teaching Hospital (TTH) in Ghana. Methodology The study was a cross-sectional study involving convenience sampling of E. coli and K. pneumoniae isolates from consenting patients’ clinical specimens, between April and June 2015. Antimicrobial susceptibility test was performed, and

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Mousse, Wassiyath, Haziz Sina, Mamadou Wele та ін. "Molecular characterization and Antibiotic resistance profiles of Escherichia coli extended-spectrum β-lactamases producer strains isolated from urine samples in Benin". European Scientific Journal, ESJ 14, № 30 (2018): 323. http://dx.doi.org/10.19044/esj.2018.v14n30p323.

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Urinary tract infections are the second common reason of medical consultations and antibiotics prescription. Escherichia coli is known to cause most urinary tract infections. The aim of this study was to characterize and determine the antibiotic resistance profile of E. coli extended-spectrum βlactamases (ESBL) producer strains isolated from urine samples. The urine samples collected came from hospitalized and non-hospitalized patient referred to Hubert Koutoukou Manga (HKM), National and University Hospital Center (Cotonou, Benin). The resistance to antibiotics was determined according to the

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Sarojamma, Vemula, and Vadde Ramakrishna. "Prevalence of ESBL-Producing Klebsiella pneumoniae Isolates in Tertiary Care Hospital." ISRN Microbiology 2011 (December 1, 2011): 1–5. http://dx.doi.org/10.5402/2011/318348.

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Extended-spectrum β lactamases (ESBLs) continue to be a major challenge in clinical setups world over, conferring resistance to the expanded-spectrum cephalosporins. An attempt was made to study the prevalence of ESBL-producing Klebsiella pneumoniae clinical isolates in a tertiary care hospital in Kurnool. A total of hundred collected isolates of Klebsiella pneumoniae was studied for their susceptibility patterns to various antibiotics and detection of ESBL producers by double disc synergy test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Of the 100 isolates tested for their

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Khan, Diwan Mahmood, I. Venkatakrishna Rao, and M. S. Moosabba. "COMPARATIVE STUDY OF GELATINASE ACTIVITY AND PELLICLE FORMATION AMONG EXTENDED-SPECTRUM BETA-LACTAMASE AND NON-EXTENDED-SPECTRUM BETA-LACTAMASE PRODUCING ACINETOBACTER BAUMANNII FROM DIABETIC FOOT ULCER INFECTIONS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 9 (2018): 496. http://dx.doi.org/10.22159/ajpcr.2018.v11i9.28336.

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Objective: The aim of the study was to assess and compare the gelatinase activity and pellicle formation in extended-spectrum beta-lactamase (ESBL) and non-ESBL producing Acinetobacter baumannii isolates from diabetic foot ulcer infection (DFI).Methods: A total of 42 isolates of A. baumannii recovered from patients of DFI from September 2016 to February 2018. Isolates were identified by the standard microbiological method and confirmed by the BD Phoenix 100 system. The antimicrobial susceptibility test was performed by the Kirby–Bauer disk diffusion method and ESBL was detected by double disk

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Al-Mayahie, Sareaa, and Jaafar JH Al Kuriashy. "Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance." Journal of Infection in Developing Countries 10, no. 06 (2016): 575–83. http://dx.doi.org/10.3855/jidc.6661.

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Introduction: Extended-spectrum β-lactamase (ESBL)-producing strains of Enterobacteriaceae are considered to be emerging pathogens. They are a major problem in hospitalized as well as community-based patients. Major outbreaks involving ESBL strains have been reported from all over the world. Recurrent urinary tract infection (UTI) is one of the risk factors for infection with ESBL-producing E. coli in hospitalized and non-hospitalized patients. Methodology: Ninety-one E. coli isolates from outpatients with recurrent UTIs were surveyed phenotypically and genotypically for ESBL production and te

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Tian, Su Fei, Bai Yi Chen, Yun Zhuo Chu та Shuang Wang. "Prevalence of rectal carriage of extended-spectrum β-lactamase-producingEscherichia coliamong elderly people in community settings in China". Canadian Journal of Microbiology 54, № 9 (2008): 781–85. http://dx.doi.org/10.1139/w08-059.

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The importance of community-acquired infections due to extended-spectrum β-lactamase-producing (ESBL) Escherichia coli has been increasingly recognized in recent years. No comprehensive data are available on the prevalence, risk factors, and genotypes of ESBL production in community residents in China. Rectal samples from 270 elderly people were collected in four communities in Shenyang (China). Colonies were screened by double-disk synergy test for ESBL production and then, ESBLs were characterized by PCR and sequencing. The clonal relatedness of all ESBL-producing isolates was determined by

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Rana, Essa muslem, H. Raheema Rana та Dawood Yasir Qasim. "Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq". World Journal of Biology Pharmacy and Health Sciences 12, № 2 (2022): 094–104. https://doi.org/10.5281/zenodo.7607147.

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Intensive care units (ICU) are epicenters for the emergence of antibiotic-resistant Gram negative bacteria and multi-resistant Gram positive infections, largely due to the inappropriate use of antimicrobials. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. The findings of the disc diffusion method used to test the isolates for &lrm;antibiotic susceptibility showed that Amikacin was the most effective &lrm;antibiotic against <em>klebsiella pneumonia</em> a

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Zurfluh, Katrin, Herbert Hächler, Magdalena Nüesch-Inderbinen та Roger Stephan. "Characteristics of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolates from Rivers and Lakes in Switzerland". Applied and Environmental Microbiology 79, № 9 (2013): 3021–26. http://dx.doi.org/10.1128/aem.00054-13.

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ABSTRACTOne of the currently most relevant resistance mechanisms inEnterobacteriaceaeis the production of enzymes that lead to modern expanded-spectrum cephalosporin and even carbapenem resistance, mainly extended-spectrum β-lactamases (ESBLs) and carbapenemases. A worrisome aspect is the spread of ESBL and carbapenemase producers into the environment. The aim of the present study was to assess the occurrence of ESBL- and carbapenemase-producingEnterobacteriaceaeand to further characterize ESBL- and carbapenemase-producingEnterobacteriaceaein rivers and lakes in Switzerland. ESBL-producingEnte

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Gharrah, Mustafa Muhammad, Areej Mostafa El-Mahdy, and Rasha Fathy Barwa. "Association between Virulence Factors and Extended Spectrum Beta-Lactamase ProducingKlebsiella pneumoniaeCompared to Nonproducing Isolates." Interdisciplinary Perspectives on Infectious Diseases 2017 (2017): 1–14. http://dx.doi.org/10.1155/2017/7279830.

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Klebsiella pneumoniaeis considered an important opportunistic multidrug-resistant pathogen. Extended spectrumβ-lactamases (ESBLs) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. This study was undertaken to compare the virulence characteristics and genetic relatedness between ESBL and non-ESBL producingK. pneumoniae. Methods. Antibiotic sensitivity test of all isolates was determined by disc diffusion assay. Phenotypic and genotypic detection of ESBL were done. Various virulence factors and some virulence factor-associated genes were scr

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Naas, T., G. Cuzon, H. Truong, S. Bernabeu та P. Nordmann. "Evaluation of a DNA Microarray, the Check-Points ESBL/KPC Array, for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and KPC Carbapenemases". Antimicrobial Agents and Chemotherapy 54, № 8 (2010): 3086–92. http://dx.doi.org/10.1128/aac.01298-09.

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ABSTRACT Extended-spectrum ß-lactamases (ESBLs) and Klebsiella pneumoniae carbapenemases (KPC carbepenemases) have rapidly emerged worldwide and require rapid identification. The Check-Points ESBL/KPC array, a new commercial system based on genetic profiling for the direct identification of ESBL producers (SHV, TEM, and CTX-M) and of KPC producers, was evaluated. Well-characterized Gram-negative rods (Enterobacteriaceae, Pseudomonas aeruginosa, Acinetobacter baumannii) expressing various ß-lactamases (KPC-2, SHV, TEM, and CTX-M types) were used as well as wild-type reference strains and isolat

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Anandkumar, H., Amaresh Nigudgi, Vinay Hajare, and Sunil Biradar. "Evaluation of different phenotypic diffusion methods in the identification of extended spectrum beta lactamase producing uropathogenic." Indian Journal of Microbiology Research 8, no. 3 (2021): 243–48. http://dx.doi.org/10.18231/j.ijmr.2021.050.

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: The study was aimed to identify the occurrence of extended spectrum of Beta lactamases (ESBLs), to compare different phenotypic methods used for the confirmation and to evaluate the antibiotic resistance pattern in ESBL producing uropathogenic : The strains were isolated from urine and the isolates resistance to at least one of the three representative cephalosporins (cefotaxime, cefpodoxime and ceftazidime) was tested for ESBL production by Double disc synergy test (DDST), Inhibitory potentiated disc diffusion (IPDD) test and quantitative E-strip method.: Of 120strains isolated, 62(51.6%) w

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Sahly, H., H. Aucken, V. J. Benedí та ін. "Increased Serum Resistance in Klebsiella pneumoniae Strains Producing Extended-Spectrum β-Lactamases". Antimicrobial Agents and Chemotherapy 48, № 9 (2004): 3477–82. http://dx.doi.org/10.1128/aac.48.9.3477-3482.2004.

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ABSTRACT The aim of this study was to determine whether there is an association between serum resistance, O serotypes, and the production of extended-spectrum β-lactamases (ESBLs) in Klebsiella pneumoniae. Ninety ESBL-producing and 178 non-ESBL-producing K. pneumoniae isolates gathered in five European countries were O serotyped and tested for sensitivity to the serum's bactericidal effect. The frequency of serum-resistant isolates was higher among ESBL-producing strains (30%; 27/90 isolates) than among non-ESBL-producing strains (17.9%; 32/178 isolates) (P = 0.037; odds ratio [OR] = 1.96; 95%

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Stone, Tyler J., James Beardsley, James Johnson, et al. "838. Drivers of empiric carbapenem use: How important is history of extended-spectrum beta-lactamase (ESBL) infection?" Open Forum Infectious Diseases 7, Supplement_1 (2020): S460. http://dx.doi.org/10.1093/ofid/ofaa439.1027.

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Abstract Background CARs are first line agents for serious infections caused by ESBL producers. Likelihood of developing subsequent ESBL infection is unknown. In patients (pts) with a history (hx) of ESBL positive (ESBLP) culture, empiric therapy with a CAR has become common in hospitals. The purpose of this study was to evaluate the microbiology of subsequent infections (SI) among pts with hx of ESBLP culture and determine risk factors associated with ESBLP SI that may justify an empiric CAR. Methods This retrospective observational study was conducted at a multicenter health system. The elec

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Begum, Momtaz, Tania Rahman, Sharmeen Sultana, Shimu Saha, and SM Shamsuzzaman. "Phenotypic Screening and Genotypic Characterization of Extended- spectrum-beta-lactamase Producing Uropathogenic Escherichia coli (UPEC) isolated from a Tertiary Care Hospital in Bangladesh." Journal of Dhaka National Medical College & Hospital 29, no. 2 (2023): 7–12. https://doi.org/10.3329/jdnmch.v29i2.80553.

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Beta-lactamases are the most important factors in the resistance to beta-lactam antibiotics among gram-negative bacteria, especially Escherichia coli. The prevalence of infections caused by extended-spectrum ß-lactamases (ESBLs)-producing Esch. coli is increasing, as one of the emerging health problems worldwide. This study aimed to investigate the prevalence of ESBL producing Esch. coli isolated from urinary tract infections (UTIs) and to observe the antibiotic resistance gene among them. Thiscross-sectional study was conducted in the Department of Microbiology, Dhaka Medical College from Jul

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Olasehinde, O., and A. Lamikanra. "Pattern of Esbls in Uro-Pathogens Obtained from a Nigerian Tertiary Hospital." Nigerian Journal of Pharmaceutical Research 16, no. 2 (2021): 139–47. http://dx.doi.org/10.4314/njpr.v16i2.16s.

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Background: Multidrug resistance remains a challenge in the treatment of Urinary Tract Infections (UTI) in Nigeria, a key factor being the occurrence of ESBL producers. Earlier reports have emphasized the occurrence of major ESBLs, little is known about the minor subtypes' occurrence in this regard.Objective: This study sought to evaluate the occurrence of major and minor ESβL producers among a cohort of uropathogens collected from a Nigerian Teaching Hospital using molecular techniques.Material and Methods: Cultures from 1000 UTI positive urine specimens were collected from the hospital labor

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Porob, Seema, Hillary A. Craddock, Yair Motro, et al. "Quantification and Characterization of Antimicrobial Resistance in Greywater Discharged to the Environment." Water 12, no. 5 (2020): 1460. http://dx.doi.org/10.3390/w12051460.

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In disenfranchised communities, untreated greywater (wastewater without sewage) is often environmentally discharged, resulting in potential human exposure to antimicrobial-resistant bacteria (ARB), including extended-spectrum beta-lactamase (ESBL) producers. We sought to examine the abundance of ARB, specifically ESBLs, and antimicrobial resistance genes (ARGs) in greywater from off-grid, pastoral Bedouin villages in Southern Israel. Greywater samples (n = 21) collected from five villages were analyzed to enumerate fecal coliforms and Escherichia coli. ESBL producers were recovered on CHROMaga

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Koirala, Sushma, Sujan Khadka, Sanjeep Sapkota та ін. "Prevalence of CTX-M β-Lactamases Producing Multidrug Resistant Escherichia coli and Klebsiella pneumoniae among Patients Attending Bir Hospital, Nepal". BioMed Research International 2021 (8 червня 2021): 1–11. http://dx.doi.org/10.1155/2021/9958294.

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The emergence of multidrug resistant (MDR) bacteria which is attributable to extended spectrum β-lactamases (ESBLs) production of CTX-M types is an obvious problem worldwide. This study is aimed at determining the prevalence of CTX-M β-lactamases producing multidrug resistant Escherichia coli and Klebsiella pneumoniae among patients attending Bir Hospital. A cross-sectional study was conducted between April and September 2019 at Bir Hospital, Kathmandu, and Department of Microbiology, National College, Kathmandu, Nepal. A total of 5,690 different clinical specimens were subjected to cultural,

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Morris, Dearbháile, Colette O'Hare, Maura Glennon, Majella Maher, Geraldine Corbett-Feeney та Martin Cormican. "Extended-Spectrum β-Lactamases in Ireland, Including a Novel Enzyme, TEM-102". Antimicrobial Agents and Chemotherapy 47, № 8 (2003): 2572–78. http://dx.doi.org/10.1128/aac.47.8.2572-2578.2003.

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ABSTRACT Organisms producing extended-spectrum β-lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing members of the family Enterobacteriaceae in Ireland. A total of 925 isolates of ampicillin-resistant members of the Enterobacteriaceae were received from six hospitals in Ireland over a 3-year period from September 1996 to September 1999. Isolates were screened for ESBL production by the double-disk diffusion (DDD) method. DDD-positive isolates that were (i) confirmed as ESBL producers by National Committee for Clinical Labora

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Kumar, Sanjeev, Chandani Surani та Meghana Chauhan. "Detection and prevalence of extended spectrum β-lactamases in gram-negative bacteria at our health-care center". National Journal of Physiology, Pharmacy and Pharmacology 13, № 5 (2023): 1. http://dx.doi.org/10.5455/njppp.2023.13.09447202201102022.

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Background: Emergence of extended spectrum β-lactamase (ESBL) producing strains of Gram-negative bacteria can lead to serious infections frequently complicates the clinical and treatment outcome. Aims and Objectives: The purpose of this study was to know the prevalence of ESBLs and to know the most common Gram-negative bacteria, which produce ESBLs at our health-care facility. Materials and Methods: This study comprised all of the isolates of Gram-negative bacteria that were acquired from various clinical samples. For the purpose of the investigation, a sample of an isolate that showed resista

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Chowdhury, Abu Hena Md Saiful Karim, Nasima Akter, and Nura Nasrin Rowshan Ara. "Detection of Imipenem Resistant Extended Spectrum B-Lactamases (Esbls) Producers Among Gram-Negative Bacilli in Chittagong Medical College Hospital, Chittagong." Journal of Chittagong Medical College Teachers' Association 28, no. 2 (2018): 69–74. http://dx.doi.org/10.3329/jcmcta.v28i2.62425.

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Background: Extended-Spectrum b-lactamases (ESBLs) producing bacteria are increasing in number and causing more severe infections because of their continuous mutation and multidrug resistance property which make its treatment difficult. The emergence of carbapenem (Imipenem) resistantant ESBLs will worsen the management of infections and increases the mortality rates. The present study was undertaken to detect the imipenem resistantant ESBLs producing bacteria in patients attending Chittagong Medical College Hospital. Materials and methods: All the isolates from different clinical samples were

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Wu, Chi-Jung, Yin-Ching Chuang, Mei-Feng Lee та ін. "Bacteremia Due to Extended-Spectrum-β-Lactamase-Producing Aeromonas spp. at a Medical Center in Southern Taiwan". Antimicrobial Agents and Chemotherapy 55, № 12 (2011): 5813–18. http://dx.doi.org/10.1128/aac.00634-11.

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ABSTRACTAlthough extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers amongAeromonasblood isolates and the genes encoding ESBLs, consecutive nonduplicateAeromonasblood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, incl

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Islam, Tashmin Afroz Binte, S. M. Shamsuzzaman, and Aleya Farzana. "Prevalence and antibiogram of ESBL producing gram negative bacilli isolated from urine in Dhaka Medical College Hospital, Bangladesh." Bangladesh Journal of Medical Microbiology 9, no. 1 (2017): 17–21. http://dx.doi.org/10.3329/bjmm.v9i1.31337.

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Extended spectrum ß-lactamase (ESBL) producing strains are resistant to a wide variety of common antimicrobials and become a major clinical concern worldwide that has complicated treatment strategies. The current study has been carried out to detect ESBL producing gram negative bacilli with their antimicrobial susceptibility pattern from urine collected over a period of 12 months from July 2011 to June 2012 from Dhaka Medical College Hospital. Samples were cultured in blood agar and MacConkey's agar media and organisms were identified by different biochemical tests such as oxidase test, reacti

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Bhattarai, Ganga Sagar, Dipendra ,. Shrestha, and Bishnu Raj Tiwari. "Extended Spectrem Beta Lactamases among Multi Drug Resistant Gram Negetive Bacilli Causing Urinry Tract Infection." Journal of Health and Allied Sciences 5, no. 1 (2019): 25–28. http://dx.doi.org/10.37107/jhas.30.

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Extended Spectrum Beta Lactamases (ESBL), the main cause of resistance to broad spectrum β-lactams, among uropathogenic bacteria have increased over time raising a global concern in the therapeutic management of infections caused by these organisms. The study was carried out in Janamaitri Hospital, Kathmandu between December 2012 to May 2013 with an objective to determine the status of ESBL producing Gram negative bacilli isolated from the urine sample, collected from patients suspected of urinary tract infection. Gram negative bacilli isolated were tested for the presence of ESBL by combined

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Padmavathy, Kesavaram, Krishnan Padma, and Sikhamani Rajasekaran. "Multidrug Resistant CTX-M-ProducingEscherichia coli: A Growing Threat among HIV Patients in India." Journal of Pathogens 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/4152704.

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Extended Spectrumβ-Lactamases (ESBLs) confer resistance to third-generation cephalosporins and CTX-M types have emerged as the most prominent ESBLs worldwide. This study was designed to determine the prevalence of CTX-M positive ESBL-producing urinaryE. coliisolates from HIV patients and to establish the association of multidrug resistance, phylogeny, and virulence profile with CTX-M production. A total of 57 ESBL producers identified among 76E. colistrains isolated from HIV patients from South India were screened forblaCTX-M, AmpC production, multidrug resistance, and nine virulence associate

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Ibrahim, Mutasim E., Mohammed Abbas, Abdullah M. Al-Shahrai та Bahaeldin K. Elamin. "Phenotypic Characterization and Antibiotic Resistance Patterns of Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Gram-Negative Bacteria in a Referral Hospital, Saudi Arabia". Canadian Journal of Infectious Diseases and Medical Microbiology 2019 (26 червня 2019): 1–9. http://dx.doi.org/10.1155/2019/6054694.

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Background. Emergence of pathogenic bacteria carrying β-lactamase-resistant determinants has become a major health problem in the hospital setting. The study aimed to determine antibiotic-resistant patterns and frequency of extended-spectrum β-lactamase- (ESBL-) producing Gram-negative bacteria (GNB) and AmpC β-lactamase-producing GNB. Methodology. A prospective cross-sectional study was conducted during a period from September 2017 to August 2018 at King Abdullah Hospital, Bisha Province, Saudi Arabia. GNB (n = 311) were recovered from patients’ clinical specimens including sputum, urine, wou

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Rao, Sridhar PN, Prasad Subba Rama, Vishwanath Gurushanthappa, Radhakrishna Manipura, and Krishna Srinivasan. "Extended-spectrum beta-lactamases producing Escherichia coli and Klebsiella pneumoniaei: A Multi-centric Study Across Karnataka." Journal of Laboratory Physicians 6, no. 01 (2014): 007–13. http://dx.doi.org/10.4103/0974-2727.129083.

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ABSTRACT Background: There are sporadic reports on detection of extended-spectrum beta-lactamases (ESBL) producers from Karnataka; hence, this is a first multicentric study across Karnataka state to determine the prevalence of ESBL production among clinical isolates of Escherichia coli and Klebsiella pneumoniaei. Aims and objectives: To determine the prevalence of ESBL producing clinical isolates of E. coli and K. pneumoniae from five geographically distributed centers across Karnataka, to study the susceptibility of ESBL producing isolates to other beta-lactam and beta-lactam-beta-lactamase i

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Brungi, Sravanthi, Sreeja Vamsi Kogila, Ramachandra Reddy Bhumi Reddy, Shaheen Sultana, and Manisha Singh. "Phenotypic detection of extended spectrum beta lactamase and metallo beta lactamase producers among multidrug resistant Escherichia coli and Klebsiella spp. in urinary tract infections." IP International Journal of Medical Microbiology and Tropical Diseases 9, no. 4 (2024): 246–52. http://dx.doi.org/10.18231/j.ijmmtd.2023.047.

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: The incidence of Urinary tract infection (UTI) concomitantly causing the morbidity and mortality in patients with specific risk factors is highly alarming. () and spp., are the most frequently isolated species and considered as highly significant due to their ability to produce Extended spectrum beta lactamase (ESBL) and Metallo beta lactamase (MBL). UTIs caused by bacteria that produces ESBL and MBL are becoming more common, and the ability of diagnostic microbiology laboratories to promptly screen for and identify these resistant infections is crucial.The main objective of my study is to i

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Bernabeu, Sandrine, Kayaththiry Caroline Ratnam, Hervé Boutal, et al. "A Lateral Flow Immunoassay for the Rapid Identification of CTX-M-Producing Enterobacterales from Culture Plates and Positive Blood Cultures." Diagnostics 10, no. 10 (2020): 764. http://dx.doi.org/10.3390/diagnostics10100764.

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We have developed a lateral flow immunoassay (LFIA), named NG-Test CTX-M MULTI (NG-Test), to detect group 1, 2, 8, 9, 25 CTX-M producers from agar plates and from positive blood cultures in less than 15 min. The NG-Test was validated retrospectively on 113 well-characterized enterobacterial isolates, prospectively on 102 consecutively isolated ESBL-producers from the Bicêtre hospital and on 100 consecutive blood cultures positive with a gram-negative bacilli (GNB). The NG-Test was able to detect all CTX-M producers grown on the different agar plates used in clinical microbiology laboratories.

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Islam, M. Saiful, M. Abdullah Yusuf, M. Badrul Islam, and Waseka Akhter Jahan. "Frequency of ESBL in Surgical Site Infection at a Tertiary Care Hospital." Journal of Current and Advance Medical Research 1, no. 2 (2014): 25–29. http://dx.doi.org/10.3329/jcamr.v1i2.20514.

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Background: Infection caused by ESBL in the surgical site infection is very alarming. Objective: The purpose of the present study was to see the status of ESBL bacteria isolated from surgical site infection with their antimicrobial sensitivity pattern.Methodology: This cross sectional study was conducted in the Department of Microbiology at Dhaka Medical College, Dhaka from January, 2005 to December, 2005 for a period of one (1) year. All the patients presented with surgical site infections at any age with both sexes were included a study population. Detection of extended spectrum beta lactama

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Vijayvergia, V., AK Sahni, M. Lall, K. Vijay, and ID Khan. "Phenotypic detection of Extended Spectrum Beta-Lactamases and Amp C Beta- Lactamases among nosocomial isolates in a tertiary care hospital." Bangladesh Journal of Medical Science 12, no. 4 (2013): 378–84. http://dx.doi.org/10.3329/bjms.v12i4.13309.

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Background & objectives: Extended-Spectrum Beta-Lactamases (ESBLs) is an important resistance mechanism in Enterobacteriaceae infections. Lack of standard guidelines from Clinical Laboratory Standards Institute (CLSI) for Amp C beta-lactamase detection poses a problem. This study was undertaken to detect ESBLs by phenotypic tests and Amp C beta-lactamase by inhibitor based method. Material and Methods: 200 consecutive non-repetitive isolates of E.coli, Klebsiella and Proteus from clinical samples were screened for ESBLs as per CLSI guidelines and confirmed by PCDT, DDST and E-tests (AB Bio

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Rawat, Vinita, Monil Singhai та Pankaj Kumar Verma. "Detection of Different β-Lactamases and their Co-existence by Using Various Discs Combination Methods in Clinical Isolates of Enterobacteriaceae and Pseudomonas spp." Journal of Laboratory Physicians 5, № 01 (2013): 21–25. http://dx.doi.org/10.4103/0974-2727.115918.

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ABSTRACT Background: Resistance to broad spectrum beta-lactams mediated by extended spectrum β-lactamase (ESBL), AmpC, and metallobetalactamase (MBLs) enzymes are an increasing problem worldwide. The study was aimed to detect occurrence rate and to evaluate different substrates and inhibitors by disc combination method for detecting varying degree of β-lactamase enzymes and their co-production. Materials and Methods: A disc panel containing imipenem (IMP), IMP/EDTA, ceftazidime (CA), ceftazidime-tazobactum (CAT), CAT/cloxacillin (CLOX), ceftazidime-clavulanic acid (CAC), CAC/CLOX, cefoxitin (C

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Bahloli, Mohammad, Hossein Samadi Kafil, Parisa Roshani Asl, Niloufar Rashidi та Seyyed Reza Moaddab. "Evaluation of Antimicrobial Susceptibility in Extended-Spectrum β-Lactamase Producing Escherichia coli Isolates From Patients in Northwest Iran". Avicenna Journal of Clinical Microbiology and Infection 11, № 1 (2024): 22–27. http://dx.doi.org/10.34172/ajcmi.3474.

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Background: Escherichia coli is one of the main causes of various diseases worldwide, whose multidrug-resistant strains have caused many public health problems by producing extended-spectrum β-lactamases (ESBLs). The resistance rate varies in different regions. Thus, it is necessary to identify ESBL-producing strains in each region and their antibiotic sensitivity in order to find appropriate treatment options. Hence, the present study aimed to detect the ESBL-producing E. coli strains and their antimicrobial susceptibility pattern in Tabriz, Iran. Methods: This study was conducted at the Imam

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Emmanuel, Ugbo, Anyamene Chris, Orji Jerry, et al. "Phenotypic Characterization and Comparative Study on ESBL-producing E. coli of Clinical Origin." International Journal of Sciences Volume 5, no. 2016-02 (2016): 27–31. https://doi.org/10.5281/zenodo.3349099.

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Extended-spectrum β-lactamases (ESBLs) continue to be a major challenge in clinical setups worldwide, conferring resistance to the expanded-spectrum cephalosporins. The present study focused on the prevalence of ESBL-producing E. coli clinical isolates among patients diagnosed of Wound and Urinary Tract Infections attending Federal Teaching Hospital Abakaliki. A total of one hundred and ninety two clinical isolates of E. coli was studied for their susceptibility patterns to cephalosporin antibiotics and detection of ESBL producers was carried out by double disc synergy test (DDST) and Brillian

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Siu, L. K., Po-Liang Lu, Po-Ren Hsueh та ін. "Bacteremia Due to Extended-Spectrum β-Lactamase-Producing Escherichia coli andKlebsiella pneumoniae in a Pediatric Oncology Ward: Clinical Features and Identification of Different Plasmids Carrying both SHV-5 and TEM-1 Genes". Journal of Clinical Microbiology 37, № 12 (1999): 4020–27. http://dx.doi.org/10.1128/jcm.37.12.4020-4027.1999.

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Thirteen patients who had 16 episodes of bacteremia were observed between 1993 and 1997 in a pediatric oncology ward with a high background isolation rate of cefotaxime- or aztreonam-resistant gram-negative bacteria. Four blood isolates were Escherichia coli and 12 were Klebsiella pneumoniae, and these isolates harbored extended-spectrum β-lactamases (ESBLs). All episodes of bacteremia were nosocomial, all except one of the episodes occurred in neutropenic patients, and all patients were treated with piperacillin or ceftazidime with amikacin and cefazolin prior to the onset of bacteremia. Nine

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P.K., Feglo,, та Adu-Sarkodie, Y. "Antimicrobial Resistance Patterns of Extended Spectrum Β-Lactamase Producing Klebsiellae and E. coli Isolates from a Tertiary Hospital in Ghana". European Scientific Journal, ESJ 12, № 30 (2016): 174. http://dx.doi.org/10.19044/esj.2016.v12n30p174.

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Introduction: High proportions of E. coli and Klebsiellae isolates at Komfo Anokye Teaching Hospital (KATH) have developed resistance to the commonly prescribed antimicrobial drugs, but the cause of which is unknown. Detailed data upon which to advocate control interventions are scanty. This study determined the prevalence of ESBL Klebsiellae and E. coli at KATH, so as to establish the linkage (if any) between ESBL production and drug resistance to antimicrobials drugs at the Komfo Anokye Teaching Hospital. Method: 405 isolates consisting of 156 E. coli strains and 234 Klebsiella pneimoniae an

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Karim Chowdhury, Abu Hena Md Saiful, Md Anwar Husain, Nasima Akter, et al. "Prevalence of Extended Spectrum b-Lactamases (ESBL) Producers Among Gram-Negative Bacilli in Wound Infection." Chattagram Maa-O-Shishu Hospital Medical College Journal 15, no. 1 (2016): 26–30. http://dx.doi.org/10.3329/cmoshmcj.v15i1.28758.

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Background: Extended Spectrum b -Lactameses Producing Organisms (ESBLs) are increasing in number and causing more severe infections because of their continuous mutation and multidrug resistance property with limited therapeutic option.Aims and Objectives: Present study was undertaken to detect the prevalence of the ESBLs producing bacteria in wound infection, so as to provide a base line data in treating them & prevent unnecessary use of antibioticsMethods: Isolated gram-negative bacteria initially screened by Minimum Inhibitory Concentration (MIC) ESBLs breakpoints. Then suspected ESBLs p

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Giriyapur, Ravi S., Namratha W. Nandihal, Krishna B. V. S., Asha B. Patil, and Chandrasekhar M. R. "Comparison of Disc Diffusion Methods for the Detection of Extended-Spectrum Beta Lactamase-Producing Enterobacteriaceae." Journal of Laboratory Physicians 3, no. 01 (2011): 033–36. http://dx.doi.org/10.4103/0974-2727.78561.

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ABSTRACTBackground: Resistance to broad-spectrum β lactams, mediated by extended-spectrum β lactamases (ESBLs), is an increasing problem world wide. This resistance poses problems for in vitro testing and reporting. Increased prevalence of ESBLs among Enterobacteriaceae creates a great need for laboratory testing methods that will accurately identify their presence.Materials and Methods: During the study, the Enterobacteriaceae isolated were tested for the presence of ESBL by the National Committee for Clinical Laboratory Standards (NCCLS) screening test, Jarlier double disc synergy (approxima

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Sonia, Suraiya Jahan, Tasnim Ahsan, Tafneen Farhana Ahmed, Shah Walia Nazneen, Kazi Hafiz Uddin, and SM Shamsuzzaman. "Prevalence of Extended Spectrum ß-Lactamase Producing Klebsiella Pneumoniae Isolates in A Tertiary Care Hospital in Bangladesh." Journal of National Institute of Neurosciences Bangladesh 6, no. 2 (2020): 101–4. http://dx.doi.org/10.3329/jninb.v6i2.50763.

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Background: Extended-spectrum β lactamases (ESBLs) continue to be a major challenge in clinical setups world over, conferring resistance to the expanded-spectrum cephalosporins. Objectives: The aim of this study was to determine the prevalence of extended spectrum β-lactamase (ESBL) in strains of Klebsiella pneumoniae isolated from different clinical specimens in Dhaka Medical College Hospital, Dhaka, Bangladesh. Methodology: This cross sectional study was carried out at the Department of Microbiology of Dhaka Medical College, Dhaka, Bangladesh from July 2016 to June 2017. Klebsiel

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Randegger, Corinne C., та Herbert Hächler. "Real-Time PCR and Melting Curve Analysis for Reliable and Rapid Detection of SHV Extended-Spectrum β-Lactamases". Antimicrobial Agents and Chemotherapy 45, № 6 (2001): 1730–36. http://dx.doi.org/10.1128/aac.45.6.1730-1736.2001.

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ABSTRACT Extended-spectrum β-lactamases (ESBLs), e.g., ESBLs of the TEM or SHV type, compromise the efficacies of expanded-spectrum cephalosporins. An SHV non-ESBL that hydrolyzes only narrow-spectrum cephalosporins can be converted into an SHV ESBL through substitutions at three amino acid positions, 179, 238, or 238–240. In order to improve detection of SHV ESBLs, a novel method, based on real-time PCR monitored with fluorescently labeled hybridization probes and followed by melting curve analysis, was developed. It is able to (i) detectbla SHV genes with high degrees of sensitivity and spec

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Nasher, Samar, Sameer Alsharapy, Anwar Al-Madhagi та Fathiah Zakham. "Epidemiology of extended-spectrum β-lactamase producing Escherichia coli from hospital settings in Yemen". Journal of Infection in Developing Countries 12, № 11 (2018): 953–59. http://dx.doi.org/10.3855/jidc.10560.

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Introduction: Infection with Extended spectrum β-lactamases (ESBLs) producing bacteria is considered as serious health problem worldwide. The aim of this cross-sectional study was to investigate the prevalence of ESBL producing Escherichia coli in hospitalized patients and the risk factors contributed for its nosocomial infections in addition to the antibiotics susceptibility patterns of isolates from 130 inpatients collected in Al Thawra General Hospital and Al-Kuwait University Hospital in Sana’a city. Methodology: Antibiotic susceptibility testing and confirmation of ESBL production w

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Parajuli, Narayan Prasad, Pooja Maharjan, Govardhan Joshi та Puspa Raj Khanal. "Emerging Perils of Extended Spectrum β-Lactamase Producing Enterobacteriaceae Clinical Isolates in a Teaching Hospital of Nepal". BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/1782835.

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Introduction. Infections due to extended spectrum β-lactamase producing Enterobacteriaceae are on the rise. They pose serious public health problems due to their resistance to large number of antibiotics. However, little is known about the genotypes of ESBL from Nepal. Therefore, the study presents results of phenotypic and molecular characterization of ESBL producing Escherichia coli and Klebsiella spp. isolated from various clinical specimens in a tertiary care teaching hospital of Nepal. Methods. A total of 172 Enterobacteriaceae clinical isolates recovered from various clinical specimens w

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Sahoo, Suryarashmi, Sarita Otta, Bichitrananda Swain, and Subrat Kumar Kar. "Detection and genetic characterization of extended-spectrum beta-lactamases producers in a tertiary care hospital." Journal of Laboratory Physicians 11, no. 03 (2019): 253–58. http://dx.doi.org/10.4103/jlp.jlp_31_19.

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Abstract BACKGROUND: Extended-spectrum beta-lactamase (ESBL)-producing organisms inactivate extended beta-lactam antibiotics and monobactams and also exhibit coresistance to many other classes of antibiotics. The present study was carried out to assess the prevalence of the ESBLs and to determine the most prevalent genotype in our hospital. MATERIALS AND METHODS: All clinically significant Gram-negative isolates were identified, and their antimicrobial susceptibility testing was done by Kirby–Bauers' disc diffusion method. ESBL detection was confirmed by minimal inhibitory concentration method

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Rajeshwari K G. "Fosfomycin susceptibility among ESBL producing gram negative bacilli causing urinary tract infections." Indian Journal of Microbiology Research 8, no. 2 (2021): 142–45. http://dx.doi.org/10.18231/j.ijmr.2021.029.

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Urinary tract infection is one of the common infection encountered in day to day practice. Due to emergence of drug resitance among uropathogens treatment options have become limited. Fosfomycin being a safe oral antibiotic is being used widely to treat multidrug resistant uropathogens. In the present study 831 (48.45%) samples that yielded significant growth were processed out of 1715 sample for ESBL detection by double disc synergy and phenotypic confirmatory method. E.coli constituted the predominant isolate (60.4%) followed by K.pneumoniae. 256 (30.80%) samples yielding growth were from ou

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Kon, Hadas, Mor Lurie-Weinberger, Adi Cohen, et al. "Occurrence, Typing, and Resistance Genes of ESBL/AmpC-Producing Enterobacterales in Fresh Vegetables Purchased in Central Israel." Antibiotics 12, no. 10 (2023): 1528. http://dx.doi.org/10.3390/antibiotics12101528.

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Beta-lactam resistance can lead to increased mortality, higher healthcare expenses, and limited therapeutic options. The primary mechanism of beta-lactam resistance is the production of extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. The spread of beta-lactamase-producing Enterobacterales via the food chain may create a resistance reservoir. The aims of this study were to determine the prevalence of ESBL/AmpC-producing Enterobacterales in vegetables, to examine the association between EBSL/AmpC-producing bacteria and types of vegetables, packaging, and markets, and to invest

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Karim Chowdhury, Abu Hena Md Saiful, Md Anwar Husain, Nasima Akter, et al. "Prevalence of Extended Spectrum b-Lactamases (ESBLs) Producers Among Gram-Negative Bacilli in Urinary Tract Infections." Chattagram Maa-O-Shishu Hospital Medical College Journal 14, no. 2 (2015): 17–20. http://dx.doi.org/10.3329/cmoshmcj.v14i2.25711.

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Background: Antimicrobial resistance is now proclaimed as the most important challenge worldwide being faced by humanity in its fight against infectious diseases. Extended Spectrum b-Lactamases (ESBLs) producing organisms are increasing in number and causing more severe infections because of their continuous mutation and multidrug resistance property which make its treatment difficult.Aims: The present study was undertaken to detect the prevalence of the ESBLs producing bacteria in urinary tract infection.Methods: Isolated gram-negative bacteria initially screened by Minimum Inhibitory Concent

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Otero, Dennise, Clay Patros, Erin McElvania, and Kamaljit Singh. "Accuracy of Nanosphere Verigene Gram-Negative Blood Culture (BC-GN) Test for Rapid Detection of Extended-Spectrum Beta-Lactamases (ESBLs) From Positive Blood Cultures." American Journal of Clinical Pathology 152, Supplement_1 (2019): S17—S18. http://dx.doi.org/10.1093/ajcp/aqz112.033.

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Abstract Background The rapid and accurate detection of ESBL production in Gram-negative rod (GNR) bacteremia is critical as recent data suggest that carbapenem treatment decreases mortality. At the same time, avoiding widespread empiric carbapenem prescribing is an important goal of antimicrobial stewardship teams. The aim of this retrospective review was to determine the accuracy of a nucleic acid–based test, Luminex Verigene BC-GN panel, to detect ESBL-positive GNRs direct from blood cultures. Methods The Verigene BC-GN was performed on all first positive GNR blood cultures. In addition, ro

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