Relevant bibliographies by topics / Escherichia coli Plasmid

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
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Academic literature on the topic 'Escherichia coli Plasmid'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Escherichia coli Plasmid"

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Rotinsulu, Sarah, Fatimawali Fatimawali, and Trina E. Tallei. "TRANSFORMASI PLASMID YANG MENGANDUNG GEN merB PADA BAKTERI Escherichia coli TOP-10." PHARMACON 8, no. 2 (2019): 290. http://dx.doi.org/10.35799/pha.8.2019.29294.

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ABSTRACT DNA transformation is the process of inserting recombinant DNA into host cells via vector plasmid. The host cell that is often used is TOP-10 Escherichia coli. The transformation method is widely used to transfer plasmids containing genetic material. This study aimed to evaluate the results of plasmid transformation containing the merB gene in the Escherichia coli TOP-10 bacteria. This study initiated with identification of the microbiology of host cells to be used, namely Escherichia coli TOP-10. Escherichia coli TOP-10 host cells were made into competent cells by transforming plasmids containing merB gene into Escherichia coli TOP-10 host cells using the heat shock method. The transformation results were evaluated by observing at the growth of Escherichia coli TOP-10 colonies on agar LB media containing ampicillin antibiotics. Plasmids on Escherichia coli TOP-10 were isolated and analyzed by 1% agarose gel electrophoresis. The results showed that the transformation of plasmids containing merB in Escherichia coli TOP-10 bacteria was successfully carried out as indicated by the growth of Escherichia coli TOP-10 bacteria on LB media agar containing ampicillin and the visualization on agarose gel resulted that the plasmid which carried the merB gene could be transformed in to the E. coli TOP-10 bacteria cell. Keywords: Transformation, Plasmids, merB genes, heat shocks, E. coli TOP-10ABSTRAK Transformasi DNA merupakan proses memasukkan DNA kedalam sel bakteri. Metode transformasi dipakai secara luas untuk mantransfer plasmid yang mengandung bahan genetika. Penelitian ini bertujuan untuk mengevaluasi hasil transformasi plasmid yang mengandung gen merB pada bakteri Escherichia coli TOP-10. Penelitian ini didahului dengan identifikasi secara mikrobiologi bakteri Escherichia coli TOP-10. Bakteri Escherichia coli TOP-10 dibuat menjadi sel kompeten yang digunakan sebagai inang. Selanjutnya dilakukan transformasi plasmid yang mengandung gen merB kedalam sel inang E. coli TOP-10 menggunakan metode heatshock. Hasil transformasi dievaluasi dengan melihat adanya koloni E. coli TOP-10 pada media LB agar yang mengandung antibiotik ampisilin. Plasmid pada E. coli TOP-10 diisolasi dan dianalisis dengan elektroforesis gel agarose 1%. Hasil penelitian menunjukkan bahwa transformasi plasmid yang mengandung gen merB pada bakteri Escherichia coli TOP-10 berhasil dilakukan, ditunjukkan dengan adanya pertumbuhan bakteri E. coli TOP-10 pada media LB agar yang mengangandung ampisilin dan hasil visualisasi pada agarose gel terlihat bahwa plasmid yang membawa gen merB dapat ditransformasikan ke dalam sel bakteri E. coli TOP-10. Kata kunci : Transformasi, Plasmid, gen merB, heatshock, E. coli TOP-10
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Johnson, Timothy J., and Lisa K. Nolan. "Pathogenomics of the Virulence Plasmids of Escherichia coli." Microbiology and Molecular Biology Reviews 73, no. 4 (2009): 750–74. http://dx.doi.org/10.1128/mmbr.00015-09.

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SUMMARY Bacterial plasmids are self-replicating, extrachromosomal elements that are key agents of change in microbial populations. They promote the dissemination of a variety of traits, including virulence, enhanced fitness, resistance to antimicrobial agents, and metabolism of rare substances. Escherichia coli, perhaps the most studied of microorganisms, has been found to possess a variety of plasmid types. Included among these are plasmids associated with virulence. Several types of E. coli virulence plasmids exist, including those essential for the virulence of enterotoxigenic E. coli, enteroinvasive E. coli, enteropathogenic E. coli, enterohemorrhagic E. coli, enteroaggregative E. coli, and extraintestinal pathogenic E. coli. Despite their diversity, these plasmids belong to a few plasmid backbones that present themselves in a conserved and syntenic manner. Thanks to some recent research, including sequence analysis of several representative plasmid genomes and molecular pathogenesis studies, the evolution of these virulence plasmids and the implications of their acquisition by E. coli are now better understood and appreciated. Here, work involving each of the E. coli virulence plasmid types is summarized, with the available plasmid genomic sequences for several E. coli pathotypes being compared in an effort to understand the evolution of these plasmid types and define their core and accessory components.
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Johnson, Timothy J., Yvonne M. Wannemuehler, Sara J. Johnson, et al. "Plasmid Replicon Typing of Commensal and Pathogenic Escherichia coli Isolates." Applied and Environmental Microbiology 73, no. 6 (2007): 1976–83. http://dx.doi.org/10.1128/aem.02171-06.

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ABSTRACT Despite the critical role of plasmids in horizontal gene transfer, few studies have characterized plasmid relatedness among different bacterial populations. Recently, a multiplex PCR replicon typing protocol was developed for classification of plasmids occurring in members of the Enterobacteriaceae. Here, a simplified version of this replicon typing procedure which requires only three multiplex panels to identify 18 plasmid replicons is described. This method was used to screen 1,015 Escherichia coli isolates of avian, human, and poultry meat origin for plasmid replicon types. Additionally, the isolates were assessed for their content of several colicin-associated genes. Overall, a high degree of plasmid variability was observed, with 221 different profiles occurring among the 1,015 isolates examined. IncFIB plasmids were the most common type identified, regardless of the source type of E. coli. IncFIB plasmids occurred significantly more often in avian pathogenic E. coli (APEC) and retail poultry E. coli (RPEC) than in uropathogenic E. coli (UPEC) and avian and human fecal commensal E. coli isolates (AFEC and HFEC, respectively). APEC and RPEC were also significantly more likely than UPEC, HFEC, and AFEC to possess the colicin-associated genes cvaC, cbi, and/or cma in conjunction with one or more plasmid replicons. The results suggest that E. coli isolates contaminating retail poultry are notably similar to APEC with regard to plasmid profiles, with both generally containing multiple plasmid replicon types in conjunction with colicin-related genes. In contrast, UPEC and human and avian commensal E. coli isolates generally lack the plasmid replicons and colicin-related genes seen in APEC and RPEC, suggesting limited dissemination of such plasmids among these bacterial populations.
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Tabassum, Tahsin, Tasmin Tabassum, Nafisa Tabassum, Syeda Muntaka Maniha, and Rashed Noor. "Stability of plasmid pBR322 within Escherichia coli cells." MOJ Biology and Medicine 6, no. 1 (2021): 17–19. http://dx.doi.org/10.15406/mojbm.2021.06.00123.

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nsertion of plasmids into the bacterial cells is of great significance especially in course of the transfer of drug resistance, virulence and other traits. Retention of plasmids within the host bacteria is therefore an important factor for bacterial homeostasis. Current study inferred the pBR322 plasmid stability within the Escherichia coli competent cells. The calcium chloride heat shock method was used for the transformation purpose. The plasmid retention phenomenon was assessed through the replica plating. The results positively showed the plasmid retention within E. coli.
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Bernadus, Zefanya G., Fatimawali Fatimawali, and Beivy Kolondam. "TRANSFORMASI PLASMID YANG MENGANDUNG GEN merB PADA Escherichia coli BL21(DE3)." PHARMACON 8, no. 1 (2019): 196. http://dx.doi.org/10.35799/pha.8.2019.29254.

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ABSTRACTDNA transformation is one of the methods for inserting DNA into bacterial cells. The current transformation method is widely used to transfer plasmids containing genetic material. This study aims to evaluate the results of plasmid transformation containing merB gene in Escherichia coli BL21(DE3) bacteria. The stages of the research carried out were preceded by the microbiological identification of the E. coli BL21(DE3) bacteria used as hosts. Then the plasmid transformation containing merB gene into the E. coli BL21(DE3) host cell using the heat shock method was carried out. The transformation results were evaluated by observing at the presence of E. coli BL21(DE3) colonies on agar Luria Bertani (LB) media containing ampicillin antibiotics. Plasmids in E. coli BL21(DE3) were isolated and analyzed by 1% agarose gel electrophoresis. The results showed the success of the transformation indicated by the growth of E. coli BL21(DE3) bacteria in agar LB media containing ampicillin and the visualization on agarose gel resulted that the plasmid which carried the merB gene could be transformed in to the E. coli BL21(DE3) bacteria.Keywords : Plasmids, merB genes, heat shock, Escherichia coli BL21(DE3)ABSTRAKTransformasi DNA merupakan salah satu metode untuk memasukkan DNA ke dalam sel bakteri. Metode transformasi saat ini dipakai secara luas untuk mentransfer plasmid yang mengandung bahan genetika. Penelitian ini bertujuan untuk mengevaluasi hasil transformasi plasmid yang mengandung gen merB pada bakteri Escherichia coli BL21(DE3). Tahapan penelitian didahului dengan identifikasi secara mikrobiologi bakteri E. coli BL21(DE3) yang digunakan sebagai inang. Selanjutnya dilakukan transformasi plasmid yang mengandung gen merB kedalam sel inang E. coli BL21(DE3) menggunakan metode heat shock. Hasil transformasi dievaluasi dengan melihat adanya koloni E. coli BL21(DE3) pada media agar Luria Bertani (LB) yang mengandung antibiotik ampisilin. Plasmid pada E. coli BL21(DE3) diisolasi dan dianalisis dengan elektroforesis gel agarose 1%. Hasil penelitian menunjukkan keberhasilan transformasi dengan adanya pertumbuhan bakteri E. coli BL21(DE3) pada media LB yang mengandung ampisillin dan hasil visualisasi pada agarose gel terlihat bahwa plasmid yang membawa gen merB dapat ditransformasikan ke dalam bakteri E. coli BL21(DE3).Kata Kunci : Plasmid, gen merB, heat shock, Escherichia coli BL21(DE3)
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Bahl, Martin Iain, Lars Hestbjerg Hansen, Tine Rask Licht, and Søren J. Sørensen. "Conjugative Transfer Facilitates Stable Maintenance of IncP-1 Plasmid pKJK5 in Escherichia coli Cells Colonizing the Gastrointestinal Tract of the Germfree Rat." Applied and Environmental Microbiology 73, no. 1 (2006): 341–43. http://dx.doi.org/10.1128/aem.01971-06.

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ABSTRACT Quantitative determination of IncP-1 plasmid loss from Escherichia coli cells colonizing the gastrointestinal tracts of germfree rats was achieved by flow cytometry. Results show that the plasmid's ability to conjugate counteracts plasmid loss and is thus an important mechanism for the stable maintenance of IncP-1 plasmids within the gastrointestinal environment.
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Arturo-Schaan, M., Z. Tamanai-Shacoori, and M. Cormier. "Stability of plasmid-borne resistance of antibiotics during starvation of Escherichia coli in raw and treated waste water and brackish water." Water Science and Technology 31, no. 5-6 (1995): 199–202. http://dx.doi.org/10.2166/wst.1995.0602.

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Cell survival and plasmid stability in Escherichia coli containing plasmids RP1, R388 and pUB824 were studied in raw waste water, treated waste water, and brackish water. The Escherichia coli strain survived well in raw and treated waste water. However, in brackish water E. coli HB101 remained culturable throughout the microcosm study, with an overall drop of 2 log units in culturability from the start to the end of the experiment. The maintenance of the three plasmids was plasmid-, and environment-dependent. Plasmid pUB824 (4 kb) was stably maintained under all conditions used in the study. Maintenance of RP1 (56 kb) and R388 (33 kb) was markedly influenced by nutritive conditions, which caused a segregation of the plasmids from cells. The results of the present study demonstrate the influence of plasmid size on plasmid stability in natural waters.
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Paganini, Julian A., Nienke L. Plantinga, Sergio Arredondo-Alonso, Rob J. L. Willems, and Anita C. Schürch. "Recovering Escherichia coli Plasmids in the Absence of Long-Read Sequencing Data." Microorganisms 9, no. 8 (2021): 1613. http://dx.doi.org/10.3390/microorganisms9081613.

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The incidence of infections caused by multidrug-resistant E. coli strains has risen in the past years. Antibiotic resistance in E. coli is often mediated by acquisition and maintenance of plasmids. The study of E. coli plasmid epidemiology and genomics often requires long-read sequencing information, but recently a number of tools that allow plasmid prediction from short-read data have been developed. Here, we reviewed 25 available plasmid prediction tools and categorized them into binary plasmid/chromosome classification tools and plasmid reconstruction tools. We benchmarked six tools (MOB-suite, plasmidSPAdes, gplas, FishingForPlasmids, HyAsP and SCAPP) that aim to reliably reconstruct distinct plasmids, with a special focus on plasmids carrying antibiotic resistance genes (ARGs) such as extended-spectrum beta-lactamase genes. We found that two thirds (n = 425, 66.3%) of all plasmids were correctly reconstructed by at least one of the six tools, with a range of 92 (14.58%) to 317 (50.23%) correctly predicted plasmids. However, the majority of plasmids that carried antibiotic resistance genes (n = 85, 57.8%) could not be completely recovered as distinct plasmids by any of the tools. MOB-suite was the only tool that was able to correctly reconstruct the majority of plasmids (n = 317, 50.23%), and performed best at reconstructing large plasmids (n = 166, 46.37%) and ARG-plasmids (n = 41, 27.9%), but predictions frequently contained chromosome contamination (40%). In contrast, plasmidSPAdes reconstructed the highest fraction of plasmids smaller than 18 kbp (n = 168, 61.54%). Large ARG-plasmids, however, were frequently merged with sequences derived from distinct replicons. Available bioinformatic tools can provide valuable insight into E. coli plasmids, but also have important limitations. This work will serve as a guideline for selecting the most appropriate plasmid reconstruction tool for studies focusing on E. coli plasmids in the absence of long-read sequencing data.
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Boyd, E. Fidelma, Charles W. Hill, Stephen M. Rich, and Daniel L. Hartl. "Mosaic Structure of Plasmids From Natural Populations of Escherichia coli." Genetics 143, no. 3 (1996): 1091–100. http://dx.doi.org/10.1093/genetics/143.3.1091.

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Abstract The distribution of plasmids related to the fertility factor F was examined in the ECOR reference collection of Escherichia coli. Probes specific for four F-related genes were isolated and used to survey the collection by DNA hybridization. To estimate the genetic diversity of genes in F-like plasmids, DNA sequences were obtained for four plasmid genes. The phylogenetic relationships among the plasmids in the ECOR strains is very different from that of the strains themselves. This finding supports the view that plasmid transfer has been frequent within and between the major groups of ECOR. Furthermore, the sequences indicate that recombination between genes in plasmids takes place at a considerably higher frequency than that observed for chromosomal genes. The plasmid genes, and by inference the plasmids themselves, are mosaic in structure with different regions acquired from different sources. Comparison of gene sequences from a variety of naturally occurring plasmids suggested a plausible donor of some of the recombinant regions as well as implicating a chi site in the mechanism of genetic exchange. The relatively high rate of recombination in F-plasmid genes suggests that conjugational gene transfer may play a greater role in bacterial population structure than previously appreciated.
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Akter, Sanjida, A. M. Masudul Azad Chowdhury, and Sohana Akter Mina. "Antibiotic Resistance and Plasmid Profiling of Escherichia coli Isolated from Human Sewage Samples." Microbiology Insights 14 (January 2021): 117863612110168. http://dx.doi.org/10.1177/11786361211016808.

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In developing countries, the occurrence of antibiotic resistance is increasing day by day and antibiotic resistant microorganisms are being found in almost every environmental setting. Plasmids are considered as the main vector in the procurement and propagation of antibiotic resistance in many microorganisms such as Escherichia coli ( E. coli). The goal of this study was to examine the antibiotic resistance and screening of plasmid in E. coli strains which were previously identified from human sewage samples. During this study antibiotic susceptibility of E. coli isolates were determined by Kirby-Bauer disk diffusion method against 5 antibiotics (ampicilin, ceftriaxone, amoxicillin, ciprofloxacin, azithromycin). Furthermore, plasmid extraction of each isolate was done according to the protocol of FavorPrepTMPlasmid Mini Kit and plasmid profiling was done by agarose gel electrophoresis. In antibiotic sensitivity test, all E. coli strains showed resistance to ampicilin, amoxicillin, and ceftriaxone. In the plasmid profiling, it was revealed that all the isolates of E. coli harbored plasmids. The plasmid sizes ranged from approximately 1.5 to 15 kb. The findings of this study prove the consequences of antibiotic resistance as well as relationship of plasmid with antibiotic resistance which necessitates proper surveillance on antibiotic usage in the developing countries.
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Dissertations / Theses on the topic "Escherichia coli Plasmid"

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McDermott, Paul Joseph. "Adaptive changes in plasmid-containing Escherichia coli." Thesis, Manchester Metropolitan University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292967.

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Chua, K.-L. "Plasmid recombination in Escherichia coli K-12." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383777.

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Derbyshire, Paul. "Plasmid maintenance in Escherichia coli K-12." Thesis, University of Warwick, 1986. http://wrap.warwick.ac.uk/100428/.

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This study investigated the maintenance of several plasmid derivatives of pBR322 and ColEl present within Escherichia coli K-12, with a view to establishing a strategy that would ensure the stable maintenance of plasmid pBR322 and the identification of a postulated partitioning function on plasmid ColEl. Two strategies were adopted. The first of these used selective nitrogen-limited chemostat culture of a glutamate-dependent strain of E.coli, carrying a pBR322 derivative expressing glutamate dehydrogenase. Using this approach, it was found that the plasmid conferred a reproductive advantage and persisted, during nitrogen limitation, for several generations beyond that of pBR322 under glucose- or phosphate- limited conditions. The second strategy used nonselective chemostat culture of a strain of E.coli carrying derivatives of pBR322 encoding stability functions from either plasmid pSClOl, the partitioning region par, or plasmid R1, the cell division/plasmid inheritance coupling region parB. Although these plasmids persisted for many generations beyond that of pBR322 under similar chemostat culture conditions, no conclusions could be made with respect to the ability of these functions to ensure stable plasmid maintenance, since the copy numbers of the respective plasmids were several-fold greater than that of pBR322, a factor that in itself would contribute to the segregational stability of the plasmids. Plasmid- free segregants which did arise were found not to be isogenic with the host strain. These mutants exhibited an increased resistance to U.V.-light irradiation, a mucoid colony phenotype, an altered cell division cycle, giving rise to minicell, filament and Y-shaped cellular morphologies, an enhanced ability to form tandemly repeated plasmid multimers and an altered sensitivity to the DNA gyrase specific antibiotics novobiocin and nalidixic acid. A study of plasmid configuration in Ion and Ion sul strains of E.coli, which share similar phenotypic characteristics with the above mutants, revealed that strains which carry a sul or azi mutation express an enhanced capacity for plasmid multimerization. Finally, no conclusive evidence could be obtained to indicate the presence of a partitioning function on plasmid ColEl. This study concludes by postulating several factors which may affect the maintenance of plasmids in E.coli K-12.
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Carbonetti, Nicholas Henry. "The aerobactin iron uptake system of plasmid ColV-K30 in Escherichia coli." Thesis, University of Leicester, 1985. http://hdl.handle.net/2381/34389.

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Some strains of Escherichia coli possess an iron uptake system, first found on a ColV plasmid, which utilises the hydroxamate siderophore aerobactin and which significantly enhances the virulence of host bacteria in experimental infections of mice. The aerobactin system of plasmid ColV-K30, cloned as the multicopy recombinant plasmid pABN1, was localised to a 7.6 kb segment of DNA by transposon mapping. In the maxicell and minicell expression systems, the cluster of genes was found to specify five polypeptides, four of which are involved in aerobactin biosynthesis, the fifth being the outer membrane receptor for ferric-aerobactin. The linear order of the genes specifying these polypeptides was determined, the gene for the receptor being at the 3' end of the cluster. The 5' and 3' transcription initiation and termination sites were located by S1 nuclease transcriptional mapping, and further transcription studies revealed a probable internal promoter within the cluster of genes, a minor unregulated promoter for the receptor gene, in addition to the major regulated promoter(s) at the 5' end of the system. Transcription of the system on ColV-K30 and subcloned plasmids, and production of the siderophore by ColV-K30, as measured by b-galactosidase production by ColV: :Mu(Ap lac) aerobactin-deficient strains, were found to be under the control of the level of freely available iron in the growth medium. The aerobactin system on pABN1, like that on ColV-K30, was shown to enhance the virulence of host bacteria in experimental infections of mice, and was found to be widespread among E. coli strains isolated from a number of extraintestinal infections of man and animals. The system was found not to be exclusively associated with ColV plasmids among these strains, and was apparently located on the chromosome of some isolates.
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Doyle, Noel James. "Plasmid mediated error prone DNA repair in Escherichia coli." Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262031.

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Friehs, Karl Hans. "Massnahmen zur Verbesserung der Produktion von rekombinanten Proteinen und Plasmid-DNS." [S.l. : s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=96397324X.

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Fletcher, Jonathan Nigel. "Plasmid-encoded virulence determinants of an enteropathogenic (0111) Escherichia coli." Thesis, University of Liverpool, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316601.

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Macpherson, Cindy Josephine. "Plasmid-mediated regulation of the E.coli cell cycle." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624602.

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Scott, David Lee Jr. "The role of dam methyltransferase in the maintenance of plasmid R6K in escherichia coli." Diss., Georgia Institute of Technology, 1994. http://hdl.handle.net/1853/25330.

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Duboff, James Steven. "The role of indole in plasmid replication in E.coli." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607877.

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Books on the topic "Escherichia coli Plasmid"

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Derbyshire, Paul. Plasmid maintenance in "Escherichia coli" K-12. typescript, 1986.

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Barrett, Siobhán. Studies on plasmid-mediated copper resistance in Escherichia coli. Universityof Birmingham, 1994.

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Molecular genetics of Escherichia coli. Guilford Press, 1989.

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Breen, Ciaran. The genetic basis of drug resistance in a pentachlorophenol degrading soil micro-organism. University College Dublin, 1998.

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Heinzen, Robert A. Characterization of plasmid and chromosomal genes from the rickettsia Coxiella Burnetii. 1991.

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Nicola, Casali, and Preston Andrew Ph D, eds. E. coli plasmid vectors: Methods and applications. Humana Press, 2003.

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(Editor), Nicola Casali, and Andrew Preston (Editor), eds. E. coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology). Humana Press, 2003.

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Weitao, Tao. From Replication Initiation to Condensation and Partition of Chromosome and Plasmid in Escherichia Coli. Uppsala Universitet, 1999.

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Wylie, John N. *. The transfer of the incompatibility group W plasmid S-a from "Escherichia coli" to "Pseudomonas aeruginosa". 1988.

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Stephens, Mark Lee. The effect of periodic operation on mixed and recombinant bacterial populations. 1989.

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Book chapters on the topic "Escherichia coli Plasmid"

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Ow, Dave Siak-Wei, Dong-Yup Lee, Hsiu-Hui Tung, and Sue Lin-Chao. "Plasmid Regulation and Systems-Level Effects on Escherichia coli Metabolism." In Systems Biology and Biotechnology of Escherichia coli. Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-1-4020-9394-4_14.

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Williams, Peter H., and Nicholas H. Carbonetti. "The Plasmid-Specified Aerobactin Iron Uptake System of Escherichia Coli." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_51.

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de Toro, María, M. Pilar Garcillán-Barcia, and Fernando de la Cruz. "Plasmid Diversity and Adaptation Analyzed by Massive Sequencing of Escherichia coli Plasmids." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch13.

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Passarinha, Luís A. "Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0872-2_7.

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Alexander, David L., Joshua Lilly, Jaime Hernandez, Jillian Romsdahl, Christopher J. Troll, and Manel Camps. "Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1053-3_3.

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Goebel, W., J. Hacker, S. Knapp, et al. "Structure, Function, and Regulation of the Plasmid-Encoded Hemolysin Determinant of Escherichia Coli." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_55.

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Crosa, J. H., L. A. Actis, Y. Mitoma, J. Perez-Casal, M. E. Tolmasky, and M. A. Valvano. "Plasmid-Mediated Iron Sequestering Systems in Pathogenic Strains of Vibrio Anguillarum and Escherichia Coli." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_52.

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Boudrant, Joseph, Baolinh Le, Frantz Fournier, and Christian Fonteix. "Modelling of Segregational Plasmid Instability of Recombinant Strain Suspension of Escherichia coli." In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9749-4_10.

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Paul, J. H. "Intergeneric Natural Plasmid Transformation between Escherichia coli and a Marine Vibrio Species." In Gene Transfers and Environment. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-77450-8_8.

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Lampel, Keith A., James A. Jagow, and Megan L. Troxell. "Oligodeoxyribonucleotide Probe Specific For The 230 Kilobase Pair Virulence Plasmid in Enteroinvasive Escherichia Coli and Shigella." In Microbial Toxins in Foods and Feeds. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0663-4_11.

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Conference papers on the topic "Escherichia coli Plasmid"

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Lopes, Marta B., Teresa Scholtz, Daniel Silva, et al. "Modelling, monitoring and control of plasmid bioproduction in Escherichia coli cultures." In 2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG). IEEE, 2012. http://dx.doi.org/10.1109/enbeng.2012.6331370.

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Lopes, M. B., K. C. Sales, V. V. Lopes, and C. R. C. Calado. "Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy." In 2013 IEEE 3rd Portuguese Meeting in Bioengineering (ENBENG). IEEE, 2013. http://dx.doi.org/10.1109/enbeng.2013.6518394.

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Scholz, Teresa, Vitor V. Lopes, and Cecilia R. C. Calado. "Monitoring bacterial processes by Fourier transform infrared spectroscopy: Helicobacter pylori drug inactivation and plasmid bioproduction in recombinant Escherichia coli cultures." In 2011 1st Portuguese Meeting in Bioengineering ¿ The Challenge of the XXI Century (ENBENG). IEEE, 2011. http://dx.doi.org/10.1109/enbeng.2011.6026090.

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Lord, S. T. "DIRECTED MUTAGENESIS OF HUMAN FIBRINOGEN: Aα CHAIN SUBSTITUTIONS THAT ALTER THROMBIN CLEAVAGE AND ANTIBODY RECOGNITION". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642887.

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Abstract:
The initial event in fibrin clot formation is the thrombin catalized cleavage of the Aa chain of fibrinogen between Argl6 and Glyl7, releasing fibrinopeptide A. Previous data indicate that most of the information required for thrombin recognition and cleavage of the Aa chain lies within the amino terminal 51 residue CNBr fragment. In order to use protein engineering techniques to study the interaction of thrombin with the Aa chain, we have constructed a plasmid expression vector which encodes a tripartite protein consisting of amino acids 1-50 of the Aa chain of human fibrinogen followed by 60 amino acids of chicken collagen, and the beta-galactosidase protein from Escherichia coli. The codons for an initiator methionine and amino acids 1-50 were assembled from 7 oligonucleotides. Protein blot analysis of bacterial lysates of cells induced to synthesize this tribrid protein show a single band (MW = 125,000) crossreactive with a monoclonal antibody, Y-18, which recognizes the Aa chain of fibrinogen but not the products of thrombin cleavage. When these lysates are incubated with thrombin, fibrinopeptide A is released as demonstrated both by protein blot analysis and radioimmunoassay. By including one heterogeneous oligonucleotide in the assembly process, we have constructed plasmids which encode specific amino acid substitutions within residues 1-23. One of these substitutions, Glyl4 to val, significantly alters both cleavage by thrombin and recognition by Y-18. Substitution of ilu for Arg23 alters neither thrombin cleavage nor monoclonal recognition while substitution of leu for Argl6 alters thrombin cleavage, but not recognition by Y-18.
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Shaobo Deng, Roger Ruan, Chul Kyoon Mok, Guangwei Huang, and Paul Chen. "NON-THERMAL PLASMA DISINFECTION OF Escherichia coli ON ALMOND." In 2005 Tampa, FL July 17-20, 2005. American Society of Agricultural and Biological Engineers, 2005. http://dx.doi.org/10.13031/2013.19589.

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Ismael, Mohammed, Ferhat Bozduman, Ali Gulec Koc, et al. "Plasma treatment for the inactivation of Escherichia coli in water." In 2015 IEEE International Conference on Plasma Sciences (ICOPS). IEEE, 2015. http://dx.doi.org/10.1109/plasma.2015.7179791.

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Elersic, Kristina, Zoran Vratnica, Danijela Vujosevic, et al. "Surface analysis of demages on escherichia coli caused by oxygen plasma radicals." In 2008 IEEE 35th International Conference on Plasma Science (ICOPS). IEEE, 2008. http://dx.doi.org/10.1109/plasma.2008.4590923.

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Ito, Masafumi, Tsuyoshi Kobayashi, Takayuki Ohta, Hiroshi Hashizume, Kenji Ishikawa, and Masaru Hori. "Main bactericidal factors of escherichia coli in solutions treated with neutral oxygen radicals." In 2016 IEEE International Conference on Plasma Science (ICOPS). IEEE, 2016. http://dx.doi.org/10.1109/plasma.2016.7534123.

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Nimbua, Suphanat, Chitsanupong Pluksa, Teerawat Temponsub, Phanuwat Thabin, Pattakorn Buppan, and Khanit Matra. "The influence of Argon, Oxygen, and Air plasma jet on Escherichia coli inactivation." In 2020 8th International Electrical Engineering Congress (iEECON). IEEE, 2020. http://dx.doi.org/10.1109/ieecon48109.2020.229566.

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Gutierrez-Leon, Diana Guadalupe, and Tomas Serrano-Ramirez. "Low Temperature Plasma by Corona discharge in water: lethal effect on Escherichia coli and Salmonella enterica serotype Typhimurium." In 2019 IEEE International Conference on Applied Science and Advanced Technology (iCASAT). IEEE, 2019. http://dx.doi.org/10.1109/icasat48251.2019.9069516.

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Reports on the topic "Escherichia coli Plasmid"

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Trezona, Thomas. Plasmid-mediated resistance to arsenite and arsenate in Escherichia coli. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.3125.

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Clark, Joshua. Determination of homology between the arsenic resistance plasmids R45 and R773 in Escherichia coli. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.5644.

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