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Dissertations / Theses on the topic 'Escherichia-Shigella'

Author: Grafiati

Published: 8 June 2024

Last updated: 31 July 2025

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1

Blanchard, Geneviève. "Versatilité nutritionnelle de l'espèce génomique "Escherichia Coli-Shigella"." Paris 5, 1990. http://www.theses.fr/1990PA05P055.

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2

Albuquerque, José Antonio Tavares de. "Análise comparativa da transcrição de genes envolvidos na invasão e escape de \'Escherichia coli\' enteroinvasora e \'Shigella flexneri\' em macrófagos J774." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-17102006-094359/.

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Escherichia coli enteroinvasora (EIEC) possuem características bioquímicas e genéticas semelhantes às de Shigella, porém para que ocorra um processo infeccioso são necessárias 102 células de Shigella em relação a 106 células de EIEC. A patogenicidade de Shigella e EIEC se dá pela presença de um plasmídio de virulência denominado pInv, que contêm os genes necessários para a invasão e disseminação bacteriana nas células do hospedeiro. Estudos anteriores relataram que os genes ipaA, ipaB, ipaC e ipaD de EIEC e Shigella não possuem diferenças genéticas que possam explicar sua diferença de patogeni

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3

Pehrson, Moysés Estevão de Souza Freitas. "Avaliação da atividade antimicrobiana de substâncias sintetizadas por cepas de Lactobacillus sp. que apresentam propriedades probióticas." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/97/97132/tde-14092016-163724/.

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As infecções causadas por patógenos intestinais gram-negativos são importantes fontes de prejuízos na criação de animais domésticos como bovinos, ovinos, suínos e aves. Em muitos casos, opta-se pela administração de antibióticos de amplo espectro para prevenção destas infecções e atenuação das perdas. No entanto, esta prática apresenta risco para a saúde humana, bem como contribui para seleção de cepas resistentes. Nos últimos anos, muito tem se discutido a respeito de novas alternativas para atenuar os prejuízos mencionados sem apresentar o mesmo risco. Uma destas alternativas é a utilização

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4

Santos, Hadassa Cristhina de Azevedo Soares dos. "Caracterização molecular e fenotípica da disseminação de diferentes sorotipos de Escherichia coli enteroinvasora em células epiteliais intestinais da linhagem Caco-2." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-16042013-101737/.

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Escherichia coli enteroinvasora (EIEC) e Shigella spp. causam disenteria bacilar, caracterizada pela destruição das células epiteliais da mucosa do cólon do hospedeiro. Ambos os microrganismos apresentam características bioquímicas, genéticas e patogênicas semelhantes, porém a doença causada por EIEC se apresenta numa forma mais branda e auto limitante. Dois genes plasmidiais, icsA e icsB estão envolvidos na disseminação intra e intercelular da bactéria, fator importante na produção e resolução da doença. Trabalhos anteriores mostraram que S. flexneri M90T possui uma capacidade maior de dissem

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5

Izabel, Hugo de Alencar. "Estudo da proteína OppA em amostras diarreiogênicas de Escherichia coli, Shigella e Salmonella." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-28012008-134840/.

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O sistema de captação de oligopeptideos (Opp), responsável pela captação de peptídeos com 3 ou mais resíduos de aminoácidos, representa um mecanismo importante de obtenção de nutrientes em bactérias. O operon Opp é constituído por 5 genes, sendo OppD e F responsáveis pela codificação dos componentes geradores, OppB e C codificantes para as proteínas que delimitam o poro da membrana e OppA que codifica o componente ligante. Neste trabalho identificamos uma alta identidade entre as proteínas OppA expressas por diferentes cepas de E. coli, 4 espécies do gênero Shigella ( 99 %) e diferentes soro

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6

Moreno, Ana Carolina Ramos. "Diferença de patogenicidade entre Escherichia coli enteroinvasora e Shigella flexneri em modelo experimental de infecção intestinal." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-25112016-142513/.

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Neste trabalho, esclarecemos tópicos da patogenicidade de EIEC que sustentam a sua menor virulência quando comparada à S. flexneri, e mostramos a importância das células dendríticas (CD) nesse processo. Estudou-se o comportamento de EIEC e S. flexneri quando em contato com células Caco-2, avaliando-se uma cinética de expressão dos genes envolvidos na invasão e disseminação bacteriana. Em geral, todos os genes foram menos expressos em EIEC, fato corroborado pelo fenótipo de disseminação bacteriana, onde EIEC foi menos eficiente do que Shigella. Também foi avaliada a modulação da resposta inflam

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7

Li, Yong. "Simultaneous detection of Escherichia coli O157:H7, Salmonella and Shigella by polymerase chain reaction-based methods /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcitt?p3144436.

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8

Silva, Gracie Luiza da. "Estudo da ação inibitória da quitosana sobre os enteropatógenos: Salmonella enterica, Shigella sonnei e Escherichia coli EPEC." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-10052006-095954/.

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Este estudo teve por objetivo avaliar a ação inibitória de duas soluções de quitosana através da determinação da concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM) de duas soluções de quitosana de diferentes procedências. A primeira solução de quitosana derivada de camarão, do tipo comercial Fluka, de PM 600.000 g/mol, G.A. 76% e a segunda solução de quitosana derivada de lula, de PM: '10 POT.7' g/mol e G.A.: 83%. As duas soluções foram ajustadas ao pH 5,0 e na concentração de 0,5% em solução acética a 1%. A melhor atividade antimicrobiana da quitosana ocorre em pH ig

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9

Pilonieta, Maria Carolina. "Transcriptional Regulation of Virulence Genes in Enterotoxigenic Escherichia coli and Shigella flexneri by Members of the AraC/XylS Family." Scholarly Repository, 2008. http://scholarlyrepository.miami.edu/oa_dissertations/111.

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Pathogenesis of enterotoxigenic Escherichia coli (ETEC) and Shigella flexneri relies predominantly on members of the AraC/XylS family of transcriptional regulators, Rns (or its homolog, CfaD) and MxiE, respectively. Rns/CfaD regulate the expression of pili, which allow the bacteria to attach to the intestinal epithelium. Better understanding of the role Rns plays in virulence was attained by expanding our knowledge of the Rns regulon, revealing that it functions as an activator of cexE, a previously uncharacterized gene. By in vitro DNase I footprinting two Rns-binding sites were identified

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10

Silva, Renée de Nazaré Oliveira da. "Caracterização molecular dos genes ospC1, ospG e ospF em diferentes sorotipos de Escherichia coli enteroinvasora." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-26042013-093005/.

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Escherichia coli enteroinvasora (EIEC) é um dos agentes etiológicos da disenteria bacilar, caracteriza-se pela destruição do epitélio do cólon provocado pela resposta inflamatória induzida após invasão da mucosa por bactérias. Cepas de EIEC são bioquímica, genética e patogênica semelhante a Shigella spp. A patogenicidade de EIEC e Shigella dependem da presença da pInv plasmídeo, que contém os genes necessários para a colonização bacteriana na mucosa intestinal. Recentemente, demonstrou-se que genes plasmidias ospC1, ospG e ospF de S. flexneri estão envolvidos na inibição da resposta inflamatór

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11

Wang, Luxin. "Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation." Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/4598.

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Thesis (M.S.)--University of Missouri-Columbia, 2006.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (Feb. 23, 2007). Includes bibliographical references.

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12

Mirza, Zainulabedeen Reda. "Control of Shigella sonnei and adhesive invasive Escherichia coli infections with a natural product which inhibits the bacterial oxidoreductase DsbA." Thesis, University of Strathclyde, 2017. http://digitool.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=28637.

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Many Gram-negative bacterial pathogens such as Shigella and adhesive invasive Escherichia coli (AIEC) cause infections characteristic of hyperinflammation. These infections require antimicrobial therapy. However, due to the widespread emergence of multiple drug-resistant strains, alternative strategies must be sought to combating infectious diseases. It has been shown that natural compounds such as propolin D are able to control Shigella growth inside host cells. Geraniol is another natural product which has a chemical structure similar to the side chain of propolin D, which possesses properti

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13

Ferreira, Lucas Gonçalves. "Caracterização da resposta inflamatória induzida por Escherichia coli enteroinvasora (EIEC) e Shigella flexneri em células epiteliais intestinais da linhagem Caco-2." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-20092017-173828/.

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Escherichia coli enteroinvasora (EIEC) e Shigella sp causam disenteria bacilar que é caracterizada pela invasão e destruição da mucosa do cólon humano. Amostras de EIEC possuem características bioquímicas, genéticas patogênicas semelhantes às espécies de Shigella, porém a doença causada por EIEC se apresenta numa forma mais branda e autolimitante. As células do epitélio intestinal participam ativamente da imunidade da mucosa, expressando e secretando uma série de mediad ores inflamatórios como citocinas, quimiocinas, moléculas de adesão e óxido nítrico. Para melhor entendimento da patogênese d

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14

Pinchaud, Katleen. "Impact d'un apport alimentaire en acide arachidonique sur le microbiote intestinal et l'axe intestin-cerveau. Conséquences pour une prévention de la maladie d'Alzheimer par les probiotiques." Electronic Thesis or Diss., Université de Lorraine, 2021. http://www.theses.fr/2021LORR0190.

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La maladie d'Alzheimer (MA) est une maladie neurodégénérative associée au vieillissement, constituant un problème de santé publique majeure dans le monde. Il n’y a actuellement aucun traitement efficace ni de moyen reconnu de prévention de cette maladie. La conception de stratégies préventives est donc indispensable pour lutter contre ces maladies. L’alimentation occidentale est particulièrement riche en acides gras ω-6, notamment en acide arachidonique (ARA), converti en nombreux médiateurs de l’inflammation dans le corps. Des travaux antérieurs du laboratoire ont montré qu’un apport alimenta

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15

Freyburger, Ludovic. "Etude de la réponse immunitaire cellulaire systémique et humorale muqueuse suite à la vaccination par la sous-unité B de la toxine de Shigella Dysenteriae comme vecteur d'antigène." Paris 5, 2007. http://www.theses.fr/2007PA05T028.

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La sous unité B de la toxine de Shiga (STxB) est un vecteur vaccinal ciblant les cellules dendritiques. Des réponses lymphocytaires T-CD4 et CD8 ainsi que la production d'anticorps ont été observées après vaccination de souris avec des protéines chimériques associant STxB couplée à différents antigènes. STxB ne favorisant pas la maturation des cellules dendritiques, nous avons évalué l'efficacité vaccinale de STxB mélangée à différents adjuvants. Ainsi, STxB couplée à différents antigènes et associée à l'aGalCer, glycolipide activant les cellules NKT, permet d'augmenter la fréquence de LT-CD8

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16

Ma, Li doctor of cellular and molecular biology. "Cellular iron metabolism and reductase systems in Escherichia coli and Shigella flexneri." Thesis, 2012. http://hdl.handle.net/2152/ETD-UT-2012-08-6160.

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The ability to acquire sufficient iron from the environment is essential for growth of most bacteria, including Escherichia coli and Shigella flexneri. In E. coli, the enterobactin-mediated iron acquisition system is the major way for the cells to get iron under iron-limiting conditions. Enterobactin is a siderophore that is synthesized and secreted in response to iron limitation to scavenge external ferric iron with high affinity. In this work, I showed that the alkyl hydroperoxide reductase (Ahp) system participates in cellular iron metabolism in both E. coli and S. flexneri. The Ahp system

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17

Desai, Stuti. "Aromatic Beta-Glucoside Utilization In Shigella Sonnei : Comparison With The Escherichia Coli Paradigm." Thesis, 2009. https://etd.iisc.ac.in/handle/2005/946.

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The aromatic beta-glucosides of plant origin, salicin and arbutin, serve as carbon sources for the sustenance of bacteria when ‘preferred’ sugars are absent in the environment. In the family Enterobacteriaceae, there are varied patterns for utilization of these beta-glucosides, wherein, in some members the ability to utilize salicin or arbutin is cryptic while in others it is completely absent. Escherichia coli harbors silent or cryptic genetic systems for the utilization of arbutin and salicin, which are activated by spontaneous mutation(s). Of these systems, the bgl operon of E.coli has be

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18

Desai, Stuti. "Aromatic Beta-Glucoside Utilization In Shigella Sonnei : Comparison With The Escherichia Coli Paradigm." Thesis, 2009. http://hdl.handle.net/2005/946.

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The aromatic beta-glucosides of plant origin, salicin and arbutin, serve as carbon sources for the sustenance of bacteria when ‘preferred’ sugars are absent in the environment. In the family Enterobacteriaceae, there are varied patterns for utilization of these beta-glucosides, wherein, in some members the ability to utilize salicin or arbutin is cryptic while in others it is completely absent. Escherichia coli harbors silent or cryptic genetic systems for the utilization of arbutin and salicin, which are activated by spontaneous mutation(s). Of these systems, the bgl operon of E.coli has be

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19

"Estudo da ação inibitória da quitosana sobre os enteropatógenos: Salmonella enterica, Shigella sonnei e Escherichia coli EPEC." Tese, Biblioteca Digital de Teses e Dissertações da USP, 2006. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-10052006-095954/.

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20

Walters, Laura Lesley. "StcE of Escherichia coli O157:H7 : characterization of substrate recognition and identification of a homolog in Shigella boydii /." 2007. http://www.library.wisc.edu/databases/connect/dissertations.html.

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21

"Análise comparativa da transcrição de genes envolvidos na invasão e escape de \'Escherichia coli\' enteroinvasora e \'Shigella flexneri\' em macrófagos J774." Tese, Biblioteca Digital de Teses e Dissertações da USP, 2006. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-17102006-094359/.

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22

Shu-Chen, Hsu, and 許淑真. "Comparison of the partial sequence of mdh genes and designing of PCR primers for the detection of Escherichia coli and Shigella spp." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/34217888630246861540.

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碩士<br>國立中興大學<br>食品科學系<br>87<br>The presence of Escherichia coli has traditionally been considered an indicator microorganism of fecal contamination in food and in environmental water, and few of them are pathogenic to cause serious disease and food poisoning. So E. coli is therefore a widely used measure of sanitary quality. The common method for detecting and quantifying E. coli requires a minimum of 5 to 7 days to obtain results after initiation of sample analysis. Incorporated MUG to culture broth (i.e MUG-LST) were developed, but it could yield false-positive and false-negative results. Th

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23

Joseph, Asha Mary. "Exploring the Evolution of Cellobiose Utilization in Shigella Sonnei And the Conservation of ChbG Orthologs in Eukaryotes." Thesis, 2016. http://etd.iisc.ac.in/handle/2005/2710.

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The chb operon constitutes the genes essential for utilization of chitooligosaccharides in Escherichia coli and related species. The six genes of the operon code for a transcriptional regulator (ChbR) of the operon, a permease (ChbBCA), a monodeacetylase (ChbG), and a phospho-beta-glucosidase (ChbF). In the absence of the substrate, the operon is maintained in a transcriptionally repressed state, while presence of the substrate leads to transcriptional activation. Regulation of the chb operon is brought about by the concerted action of three proteins, the negative regulator NagC coded by the n

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24

Joseph, Asha Mary. "Exploring the Evolution of Cellobiose Utilization in Shigella Sonnei And the Conservation of ChbG Orthologs in Eukaryotes." Thesis, 2016. http://etd.iisc.ernet.in/handle/2005/2710.

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The chb operon constitutes the genes essential for utilization of chitooligosaccharides in Escherichia coli and related species. The six genes of the operon code for a transcriptional regulator (ChbR) of the operon, a permease (ChbBCA), a monodeacetylase (ChbG), and a phospho-beta-glucosidase (ChbF). In the absence of the substrate, the operon is maintained in a transcriptionally repressed state, while presence of the substrate leads to transcriptional activation. Regulation of the chb operon is brought about by the concerted action of three proteins, the negative regulator NagC coded by the n

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25

Chigayo, K. "A study of the chemical components of extracts from kirkia wilmsii and an investigation into their properties." Diss., 2015. http://hdl.handle.net/11602/272.

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26

Ledwaba, Solanka Ellen. "Prevalence of selected bacterial and viral entero-pathogens in children less than 5 years of age in Limpopo Province, South Africa." Diss., 2016. http://hdl.handle.net/11602/779.

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27

Phuti, Moukangoe Getrude. "Synthesis, characterization and antimicrobial activity of cobalt and cobalt sulphide nanoparticles against selected microbes that are found in wastewater." Thesis, 2018. http://hdl.handle.net/10352/447.

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M. Tech (Department of Biotechnology, Faculty of Applied and Computer Sciences) Vaal University of Technology.<br>Water shortages, water pollution and climate changes are highly interrelated global issues. These have raised immense concerns about serious adverse effects on the quality, treatment and re-use of wastewater. A major role of water is for vitality of life on earth. Water is recognized as source of evolution from origin to degree of civilization, since it is an essential resource its treatment becomes a necessity for day to day for life. Nanoparticles and their application in trea

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