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Journal articles on the topic 'Escherichia-Shigella'

Author: Grafiati

Published: 8 June 2024

Last updated: 31 July 2025

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1

Ferdi, Roni, Irsan Saleh, Theodorus Theodorus, and Salni Salni. "Uji Efek Antibakteri Propolis Terhadap Escherichia Coli Dan Shigella Dysenteriae Secara In Vitro." Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya 5, no. 2 (2019): 52–61. http://dx.doi.org/10.32539/bji.v5i1.7982.

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Bakteri yang sering menjadi penyebab diare infeksi adalah Escherichia coli dan Shigella dysenteriae. Penggunaan antibiotik yang berlebihan dan kurang rasional pada kasus diare mendorong terjadinya perkembangan resistensi patogen multi obat. Propolis merupakan salah satu solusi untuk mengatasi infeksi diare. Tujuan penelitian ini untuk mengetahui kemampuan efek antibakteri ekstrak propolis terhadap bakteri Escherichia coli dan Shigella dysenteriae dalam pengujian secara in vitro dengan berbagai konsentrasi. Penelitian eksperimen laboratorium secara in vitro. Sampel dalam penelitian ini adalah b

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Novalina, Dhiah, Sugiyarto Sugiyarto, and Ari Susilowati. "Aktivitas Antibakteri Kulit Buah Karika Dieng terhadap Shigella flexneri dan Escherichia coli." Jurnal Teknologi Laboratorium 7, no. 2 (2018): 53–60. http://dx.doi.org/10.29238/teknolabjournal.v7i2.137.

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Karika merupakan tanaman endemik Dataran Tinggi Dieng. Daun Karika telah diteliti memiliki aktivitas antibakteri terhadap bakteri penyebab diare, sedangkan kulit buah dibuang atau dijadikan campuran pakan ternak. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit buah Karika terhadap bakteri penyebab keracunan, Shigella flexneri dan Escherichia coli. Sampel difraksinasi untuk memperoleh fraksi n-heksan dan etil asetat. Fraksi diuji aktivitas antibakteri nya terhadap bakteri Shigella flexneri dan Escherichia coli dengan metode sumuran. Hasil penelitian menunjukkan bahwa fraks

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3

Siregar, Beladiena Citra, Welly Darwis, and Mardhatillah Sariyanti. "Uji Efektivitas Ekstrak Akar Tanaman Lauh Putiah (Ficus racemosa L.) Terhadap Bakteri Escherichia coli dan Shigella dysenteriae Penyebab Diare." Jurnal Kedokteran RAFLESIA 5, no. 1 (2019): 53–63. http://dx.doi.org/10.33369/juke.v5i1.8778.

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ABSTRAKLatar Belakang: Diare merupakan salah satu masalah kesehatan di dunia dan negara berkembang. Mortalitas diare berkisar 17,5-21% dengan ekuivalent 1,5 juta orang setiap tahunnya. Escherichia coli dan Shigella Sp. merupakan patogen utama penyebab diare. Antibioik siprofloksasin yang digunakan untuk mengobati diare memiliki berbagai efek samping. Pemanfaatan tanaman obat merupakan salah satu alternatif untuk mengatasi masalah tersebut. Akar Lauh Putiah merupakan salah satu tanaman obat tradisional yang mempunyai banyak manfaat, salah satunya sebagai antibakteri. Penggunaan akar pada masyar

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Dewi, Lulu Fatma, Sartini Sartini, and Rahmiati Rahmiati. "Isolasi Bakteri Asam Laktat dari Usus Sapi (Bos taurus) serta Kemampuannya dalam Menghambat Pertumbuhan Bakteri Eschericia coli dan Shigella sp." Jurnal Ilmiah Biologi UMA (JIBIOMA) 1, no. 1 (2019): 21–27. http://dx.doi.org/10.31289/jibioma.v1i1.145.

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The purpose of this research was to determine the ability of BAL from cattle intestine (Bos taurus) in inhibiting the growth of Eschericia coli and Shigella sp. Testing of BAL capability in inhibiting growth of Escherichia coli and Shigella sp. using the disc-diffusion method or the Kirby-Bauer method, which was done by measuring the inhibit zone around the paper disc. Data were analyzed descriptively by displaying data in table and picture form. The results obtained 2 isolates of BAL from the cow intestine. All isolates showed positive results when tested for antibacterial against Escherichia

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Rabbana, Rahmawati, Rachmat Kosman, and Siska Nuryanti. "Antibacterial Activity Test of Java Wood (Lannea coromandelica (Houtt.) Merr.) Ethanol Extract Against Bacteria Causing Gastrointestinal Infections Using Agar Diffusion Method." Makassar Pharmaceutical Science Journal (MPSJ) 1, no. 2 (2023): 66–75. https://doi.org/10.33096/mpsj.v1i2.41.

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The purpose of this research was to determine the antibacterial activity of the ethanol extract of Javanwood leaves Lannea coromandelica (Houtt.) Merr.) against bacteria that cause digestive tract infections. The research method begins with the extraction process using the maceration method with 96% ethanol solvent and evaporated using a rotary evaporator. Java wood leaf ethanol extract was subjected to screening tests with a concentration of 0.1%; 0.5%; and 1% against the test bacteria Eschericia coli, Salmonella typhi, Shigella dysenteriae and Vibrio cholerae. MIC and MBC tests were carried

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6

Denita, Alvina Via, Ainur Rofieq, H. Husamah, and Abdulkadir Rahardjanto. "Analysis of Bacteria Escherichia coli, Salmonella sp and Shigella sp on Black Sticky Rice Ice in Malang." Mangifera Edu 6, no. 2 (2022): 169–81. http://dx.doi.org/10.31943/mangiferaedu.v6i2.129.

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Es tape ketan hitam is a traditional drink that is in great demand by the public and has the potential as a source of disease transmission is in the process of processing not using proper hygiene sanitation. The purpose of this research is to determine the content of Escherichia coli, Salmonella sp., and Shigella sp. in es tape ketan hitam. The research was conducted by planting Escherichia coli on EMBA and planting Salmonella sp., and Shigella sp. on SSA. The technique used is the spread method. The results of this study indicate that samples 1, 2, 5 and, 6 contain Escherichia coli exceeding

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7

Suwandi, Edy. "Pengaruh Air Rebusan Rimpang Jeringau Merah (Acorus Calamus L.) Konsentrasi 100%, 75%, 50%, Dan 25% terhadap Sensitifitas Bakteri Escherichia Coli, Salmonella Typhi dan Shigella sp." Jurnal Laboratorium Khatulistiwa 2, no. 2 (2019): 81. http://dx.doi.org/10.30602/jlk.v2i2.327.

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Abstract: Red Jeringau (Acorus calamus L.) is one of the endemic plants of West Kalimantan which contains antibacterial, phytochemical, and antioxidant activity. This plant has been used for generations by people who live in the interior and away from health services as a mixture of traditional medicines. This study aims to determine the effect of the concentration of Jeringau Red rhizome water on the sensitivity of Escherichia coli, Salmonella typhi and Shigella sp with concentrations of 100%, 75%, 50%, and 25%. The Red Jeringau Rhizome is obtained from the Landak Regency and then boiled usin

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Azmuda, Nafisa, Rabeya Bilkis, Humaira Akter, Anowara Begum, Sirajul Islam Khan, and Nils Kåre Birkeland. "Serological cross-reactivity of environmental Escherichia coli strains with Shigella-specific antisera." Bangladesh Journal of Microbiology 33, no. 1-2 (2018): 29–33. http://dx.doi.org/10.3329/bjm.v33i1.39600.

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Many bacteria of clinical and environmental origin show evidence of sharing common surface antigens. The present study aimed for isolation of Escherichia coli strains that were serologically cross-reactive with Shigella species from freshwater ecosystems in Bangladesh by conventional cultural methods. Among twenty eight isolates, two isolates, termed 12(35) and 6(50) showed cross-reactivity with four polyvalent serogroup-specific Shigella antisera using slide agglutination assay. The isolates were identified and charcterized by cultural and biochemical properties and Western blot analysis. The

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Nguyen, Trung Vu, Phung Van Le, Chinh Huy Le, and Andrej Weintraub. "Antibiotic Resistance in Diarrheagenic Escherichia coli and Shigella Strains Isolated from Children in Hanoi, Vietnam." Antimicrobial Agents and Chemotherapy 49, no. 2 (2005): 816–19. http://dx.doi.org/10.1128/aac.49.2.816-819.2005.

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ABSTRACT The MICs for 162 diarrheagenic Escherichia coli strains and 28 Shigella strains were determined on the basis of NCCLS guidelines. More than 75% of the strains were resistant to ampicillin, chloramphenicol (53.6% of Shigella strains), and trimethoprim-sulfamethoxazole. Multiresistance was detected in 89.5% of E. coli strains and 78.6% of Shigella strains.

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Hyma, Katie E., David W. Lacher, Adam M. Nelson, et al. "Evolutionary Genetics of a New Pathogenic Escherichia Species: Escherichiaalbertii and Related Shigellaboydii Strains." Journal of Bacteriology 187, no. 2 (2005): 619–28. http://dx.doi.org/10.1128/jb.187.2.619-628.2005.

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ABSTRACT A bacterium originally described as Hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic Escherichia coli. Subsequent studies identified similar H. alvei-like strains that are positive for an intimin gene (eae) probe and, based on DNA relatedness, are classified as a distinct Escherichia species, Escherichia albertii. We determined sequences for multiple housekeeping genes in five E. albertii strains and compared these sequences to those of strains representing the major groups of pathogenic E.

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11

Kheir, Kheir M., Bernard Mbwele, Khadija N. Omar, Modester D. Nkungu, and Lucy A. Namkinga. "Prevalence and Antimicrobial Susceptibility Patterns of Salmonella Species, Shigella Species and Escherichia coli among Children Suffering from Diarrhoea in Unguja - Zanzibar, Tanzania." Journal of Advances in Microbiology 24, no. 6 (2024): 39–47. http://dx.doi.org/10.9734/jamb/2024/v24i6831.

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Aims: This study aimed at providing evidence of the prevalence and antimicrobial susceptibility patterns towards Salmonella spp., Shigella spp. and Escherichia coli among under-five children in Unguja – Zanzibar, Tanzania. Study Design: Cross sectional design was used to collect samples from stool of children suffering from diarrhoea. Place and Duration of Study: The study was carried out in Zanzibar west urban region between October, 2019 to February, 2020. Methodology: A cross-sectional study was conducted from October 2019 to February 2020. Random samples were collected to investigate the p

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12

Masyhuda, Irmawati, and Nurul Azmah Nikmatullah. "Earthworm Infusion as an Antimicrobial Against Bacteria Causing Diarrhea." Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan 18, no. 1 (2024): 30–35. http://dx.doi.org/10.33533/jpm.v17i2.6649.

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Diarrhea is the second most common disease worldwide after Acute Respiratory Infection (ARI). One of the causes of diarrhea is the gastrointestinal infection caused by disease bacteria (microbe) such as Salmonella sp., Escherichia coli, Shigella sp., and Vibrio cholerae. The earthworm species Lubricus sp can be used as an alternative medication believed to be able to cure digestive diseases such as typhus, dysentery, and other indigestion like gastritis. This research is laboratory experimental research. The antibacterial activity is measured by determining the Minimum Inhibitory Concentration

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13

Amos, Dora, Priya Khanna, Sayeed Adnan Aali, and Guduru Gopal Rao. "Is whole genome sequencing the answer for identifying Shigella bacteraemia?" BMJ Case Reports 12, no. 12 (2019): e231596. http://dx.doi.org/10.1136/bcr-2019-231596.

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We present a rare case of Shigella flexneri bacteraemia and the challenges of differentiating Escherichia coli and Shigella spp using conventional and newer laboratory techniques in diagnostic laboratories. The organism was identified only after whole genomic sequencing .

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14

Elzuhria A, Nadia, Nur Silmi Kaffah, Nafis Rizqi N, et al. "ANTIBIOTICS SENSITIVITY TEST DIFFUSION AND DILUTION METHODS." Journal of Research in Pharmacy and Pharmaceutical Sciences 2, no. 1 (2023): 38–47. http://dx.doi.org/10.33533/jrpps.v2i1.7027.

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An antibiotic sensitivity test is a method to test the response of bacteria to antibiotics. This study aims to determine the effectiveness of an antibiotic against microbial activity. The sensitivity test was conducted with two methods, namely, the diffusion method and the dilution method. The diffusion method was performed using paper discs (Kirby-Bauer) against Escherichia coli and Shigella sonnei bacteria with amoxicillin, neomycin, and sulfonamide antibiotics. The data required is the diameter of the inhibition zone. Results showed that Escherichia coli was sensitive to amoxicillin but res

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Rahayu, Nunik Tri, Ai Sri Nurhasanah, Alfi Rumidatul, Feldha Fadhila, and Yayan Maryana. "Pengujian Aktivitas Antibakteri Ekstrak Kulit Ranting Sengon Dengan Pelarut Metanol Dan N-Heksana." Jurnal Mitra Kesehatan 3, no. 1 (2020): 6–15. http://dx.doi.org/10.47522/jmk.v3i1.82.

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Pohon Sengon (Falcataria moluccana) memiliki senyawa metabolit sekunder yaitu terpenoid, steroid, flavonoid, fenolik, tanin, saponin yang berfungsi sebagai antibakteri. Antibakteri bersumber dari alam menjadi alternatif untuk pengobatan penyakit infeksi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kulit ranting Sengon dengan pelarut n-heksana dan metanol terhadap Shigella dysentriae, Klebsiella pneumoniae, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, dan Proteus mirabilis. Penelitian ini menggunakan metode Kirby Bauer dengan kon

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Rahayu, Nunik Tri, Ai Sri Nurhasanah, Alfi Rumidatul, Feldha Fadhila, and Yayan Maryana. "PENGUJIAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT RANTING SENGON (Falcataria moluccana) DENGAN PELARUT METANOL DAN N-HEKSANA." Jurnal Mitra Kesehatan 3, no. 1 (2020): 5–10. http://dx.doi.org/10.47522/jmk.v3i1.44.

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Pendahuluan: Pohon Sengon (Falcataria moluccana) memiliki senyawa metabolit sekunder yaitu terpenoid, steroid, flavonoid, fenolik, tanin, saponin yang berfungsi sebagai antibakteri. Antibakteri bersumber dari alam menjadi alternatif untuk pengobatan penyakit infeksi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kulit ranting Sengon dengan pelarut n-heksana dan metanol terhadap Shigella dysentriae, Klebsiella pneumoniae, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, dan Proteus mirabilis. Metode: Penelitian ini menggunakan metode K

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17

Rudiah, Sukhriatun. "Antibacterial Potential of Ethanol Extract from White Pidada (Sonneratia alba) Leaves Against Gastrointestinal Pathogens." Journal Microbiology Science 4, no. 2 (2024): 271–78. http://dx.doi.org/10.56711/jms.v4i2.1123.

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The antibacterial properties of the ethanol extract of White Pidada leaves (Sonneratia alba), a mangrove plant rich in bioactive compounds such as phenols, flavonoids, steroids, saponins, triterpenoids, tannins, and alkaloids, were evaluated in this study. The research focused on determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against gastrointestinal infection-causing bacteria, including Salmonella typhii, Shigella dysenteriae, Escherichia coli, and Vibrio cholerae. The MIC results showed effective inhibition at concentrations as low as 0.02

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Warsinah, Warsinah, Triyadi Hendra Wijaya, and Nuraeni Ekowati. "The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction." Journal of Science and Technology Research for Pharmacy 1, no. 2 (2022): 7–15. http://dx.doi.org/10.15294/jstrp.v1i2.49615.

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Background: Recently, with the high prevalence of diarrhoea caused by bacterial infection, the usage of antibiotics has increased. Antibiotic overuse might lead to several side effects and resistance, suggesting the development of an alternate antibacterial agent. A mangrove plant, Acanthus Illinois, contained triterpenoid, which has antibacterial properties. Aim: This study aimed to evaluate the antibacterial effect of the Acanthus Illinois n-hexane fraction against Escherichia coli and Shigella dysenteriae. Method: The Acanthus Illinois was fraction using n-Hexane, identification

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Sitompul, Erly, Ahmad Hafizullah, Yosy Cinthya Eriwaty Silalahi, and Realisni Telaumbanua. "UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI PINANG (Areca catechu L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli, Shigella dysentriae, DAN Salmonella typhi." JURNAL FARMANESIA 4, no. 2 (2017): 79–83. http://dx.doi.org/10.51544/jf.v4i2.2705.

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One of the medicinal plants is the areca nut (Areca catechu L.) from the Arecae family which has the potential as an anticancer having antioxidant and antimutagenic, astringent, and anthelmintic effects. Areca nut seeds contain alkaloids, such as arekoline, arekolidine, arekain, guvakolin, guvasine and isoguvasine. This study aims to determine the characteristics of the betel nut (Areca catechu L.) simplicia, class of compounds, and the antibacterial activity of the ethanol extract of areca nut (Areca catechu L.) against the growth of Escherichia coli, Shigella dysentriae, and Salmonella typhi

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Nasution, Parlindungan, Sugito Sugito, and Kuswiyanto Kuswiyanto. "Pengaruh Konsentrasi Ekstrak Metanol Daun Sambiloto (Andrographis Paniculata Ness) terhadap Sensitivitas Enterobacteriaceae dengan Metode Difusi." Jurnal Laboratorium Khatulistiwa 2, no. 1 (2019): 26. http://dx.doi.org/10.30602/jlk.v2i1.323.

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Abstract: Sambiloto is plant one of the natural resources that be come important to used as medicine. Sambiloto effcacy had been known since long time ago. Based on it’s pharmacological properties, Sambiloto leaves provide antidiarrheal activity against bacteria that causing diarehea in humans especially Eacherichia coli and Shigella dysentriae bacteria. The main content of Sambiloto leaves are diterpenoid lactones (andrographolide), paniculides, farnesols and ﬂavonoids. The purpose of this research was to study the effect of the methanol extract concentration of Sambiloto leaves toward the se

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Hanum, Zuraida, Rastina Rastina, and Veronica Wanniatie. "Kemampuan Antibakteri Susu Fermentasi terhadap Escherichia coli dan Shigella." Jurnal Agripet 17, no. 1 (2017): 24–30. http://dx.doi.org/10.17969/agripet.v17i1.6572.

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ABSTRAK. Penelitian ini dilakukan dengan menggunakan starter Lactobacillus plantarum pada konsentrasi 3%, 4% dan 5% dan diinkubasi selama 48 jam pada suhu kamar. Pengamatan terhadap susu fermentasi dilakukan selama 8 hari. Uji antibakteri ini dilakukan untuk melihat apakah susu fermentasi tersebut mampu menekan pertumbuhan patogen enterobakteri. Selanjutnya dilakukan pengujian aktivitas penghambatan mikroba dari susu fermentasi terhadap bakteri patogen dengan tiga kali ulangan. Data yang diperoleh dianalisis secara statistik menggunakan ANOVA. Hasil pengamatan menunjukkan bahwa kemampuan susu

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Telaumbanua, Realisni, and Ulfayani Mayasari. "UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI PINANG (ARECAE SEMEN) TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI, SHIGELLA DYSENTRIAC, DAN SALMONELLA TYPHI." KLOROFIL: Jurnal Ilmu Biologi dan Terapan 5, no. 2 (2021): 117. http://dx.doi.org/10.30821/kfl:jibt.v5i2.9370.

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<p class="abstrak">This study aims to determine the characteristic of simplisia areca nut (<em>Arecae semen</em>), the class of compounds antibacterial activity determination of ethanolic extract of areca nut (<em>Arecae semen</em>) against <em>Escherichia coli, Shigella dysentriae,</em> dan <em>Salmonella typhi</em>. The method used is ethanol extract of areca nut obtained from maceration process with ethanol 96%. Antibacterial activity testers by the diffusion method in order to use paper discs against <em>Escherichia coli</em&gt

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23

S, Ebrahimi, Jalali H, and Ziaolhagh SH. "Evaluating the antimicrobial effects of turmeric, ginger and clove extracts on some food pathogen." MOJ Food Processing & Technology 11, no. 1 (2023): 21–24. http://dx.doi.org/10.15406/mojfpt.2023.11.00274.

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The aim of this study was to investigate the antibacterial effects of hydroalcoholic extracts of turmeric, ginger and clove on food pathogens including Salmonella Typhimurium, Shigella Dysenteriae and E. coli. To determine the antimicrobial effects, certain volumes of hydroalcoholic extracts of turmeric, ginger and clove were added to the blank disks, and after culturing the bacteria in Müller-Hinton Agar culture medium, the disks were placed on the culture medium and after the time required for the growth of the microorganism, the cultures were examined for the diameter of the growth inhibiti

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Beld, M. J. C., and F. A. G. Reubsaet. "Differentiation between Shigella, enteroinvasive Escherichia coli (EIEC) and noninvasive Escherichia coli." European Journal of Clinical Microbiology & Infectious Diseases 31, no. 6 (2011): 899–904. http://dx.doi.org/10.1007/s10096-011-1395-7.

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Selvam, Dharshini, Nor Ismaliza Mohd Ismail, and Michelle Y. T. Ng. "Potential of a novel and thermostable recombinant phage endolysin of Escherichia phage KW1E_UTAR against Shigella spp." E3S Web of Conferences 603 (2025): 01015. https://doi.org/10.1051/e3sconf/202560301015.

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Shigella causes millions of cases and hundreds of thousands of deaths annually, mainly affecting children in impoverished countries. Shigellosis outbreaks have been linked to waterborne transmission, and chlorination is commonly used to disinfect water. However, the emergence of chlorine-resistant Shigella spp. is a concern. Therefore, it is imperative to develop an alternative antibacterial agent such as endolysin against the Shigella spp. In this study, the Escherichia phage KW1E_UTAR gene encoding for endolysin was successfully cloned and expressed in Escherichia coli pET-28a. The purified

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Lee, C. K., S. J. Oh, H. J. Kim, et al. "P919 Gut microbial signature and phenotype-specific Indicators in fecal microbiota of Korean patients with Inflammatory Bowel Disease." Journal of Crohn's and Colitis 17, Supplement_1 (2023): i1028. http://dx.doi.org/10.1093/ecco-jcc/jjac190.1049.

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Abstract Background The incidence and prevalence of inflammatory bowel disease (IBD) in Asian countries have been increasing rapidly over the last few decades. This study was aimed to reveal gut metagenomic characteristics and identify a robust fecal microbial marker in Korean patients with IBD through a large-scale comparative study. Methods Gut metagenomic study using 640 fecal samples collected from patients with 523 IBD (223 Crohn’s disease [CD], 300 ulcerative colitis [UC]) and 117 healthy controls was conducted by 16S rRNA sequencing. Unsupervised cluster analysis based on the predicted

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Luck, Shelley N., Sally A. Turner, Kumar Rajakumar, Ben Adler, and Harry Sakellaris. "Excision of the Shigella Resistance Locus Pathogenicity Island in Shigella flexneri Is Stimulated by a Member of a New Subgroup of Recombination Directionality Factors." Journal of Bacteriology 186, no. 16 (2004): 5551–54. http://dx.doi.org/10.1128/jb.186.16.5551-5554.2004.

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ABSTRACT Pathogenicity islands are capable of excision and insertion within bacterial chromosomes. We describe a protein, Rox, that stimulates excision of the Shigella resistance locus pathogenicity island in Shigella flexneri. Sequence analysis suggests that Rox belongs to a new subfamily of recombination directionality factors, which includes proteins from P4, enterohemorrhagic Escherichia coli, and Yersinia pestis.

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Prunier, Anne-Laure, Raymond Schuch, Reinaldo E. Fernández, and Anthony T. Maurelli. "Genetic Structure of the nadA and nadB Antivirulence Loci in Shigella spp." Journal of Bacteriology 189, no. 17 (2007): 6482–86. http://dx.doi.org/10.1128/jb.00525-07.

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ABSTRACT Comparison of nadA and nadB in 14 Shigella strains and enteroinvasive Escherichia coli versus E. coli showed that at least one locus is altered in all strains. These observations explain the characteristic nicotinic acid auxotrophy of Shigella organisms and are consistent with the previously identified antivirulence nature of these genes for these pathogens.

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Lan, Ruiting, M. Chehani Alles, Kathy Donohoe, Marina B. Martinez, and Peter R. Reeves. "Molecular Evolutionary Relationships of Enteroinvasive Escherichia coli and Shigella spp." Infection and Immunity 72, no. 9 (2004): 5080–88. http://dx.doi.org/10.1128/iai.72.9.5080-5088.2004.

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ABSTRACT Enteroinvasive Escherichia coli (EIEC), a distinctive pathogenic form of E. coli causing dysentery, is similar in many properties to bacteria placed in the four species of Shigella. Shigella has been separated as a genus but in fact comprises several clones of E. coli. The evolutionary relationships of 32 EIEC strains of 12 serotypes have been determined by sequencing of four housekeeping genes and two plasmid genes which were used previously to determine the relationships of Shigella strains. The EIEC strains were grouped in four clusters with one outlier strain, indicating independe

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Rai, Suman, and Shiva Kumar Rai. "Anti-Bacterial Effect of Zanthoxylum armatum Oleoresin Against Some Bacterial Isolates from Pork and Chicken Meat Sold in Dharan, Nepal." Himalayan Journal of Science and Technology 1 (December 1, 2017): 35–39. http://dx.doi.org/10.3126/hijost.v1i0.25820.

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Zanthoxylum armatum is a medicinal plant found in the Himalayan range. The present study was carried out to unravel the antimicrobial activities of Z. armatum oleoresin against bacteria isolated from raw pork and chicken meat sold in Dharan submetropolitan city. Five bacterial species Salmonella enterica var Typhi, Escherichia coli, Shigella dysenteriae, Bacillus cereus and Staphylococcus aureus were isolated. The antimicrobial activity of oleoresin extracted from Z. armatum was tested by agar well diffusion method and MICs were compared with standard antibiotics against isolated bacteria. The

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Shafiee, Darya, Zhandos Salpynov, Arnur Gusmanov, Yerkhanat Khuanbai, Zhussipbek Mukhatayev, and Jeannette Kunz. "Enteric Infection-Associated Reactive Arthritis: A Systematic Review and Meta-Analysis." Journal of Clinical Medicine 13, no. 12 (2024): 3433. http://dx.doi.org/10.3390/jcm13123433.

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Background. The objective of this systematic review and meta-analysis was to estimate the proportions of individuals infected with Campylobacter, Escherichia, Salmonella, Shigella, or Yersinia who develop reactive arthritis. Methods. A systematic review was conducted, encompassing English-language articles published before January 2024, sourced from the Embase, PubMed, Scopus, and Web of Science databases. This review included observational studies that reported the occurrence of reactive arthritis (ReA) among patients with Campylobacter, Escherichia, Salmonella, Shigella, or Yersinia infectio

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Sari, Felisa Ananda, Herwin Herwin, and Selpida Handayani. "Antibacterial Activity of Ethanol Extract of Purple Kencana Leaves (Ruellia tuberosa L.) Using TLC-Bioautography and Agar Diffusion Methods." Journal Microbiology Science 3, no. 2 (2023): 30–38. http://dx.doi.org/10.56711/jms.v3i2.982.

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Kencana Ungu Leaf (Ruellia tuberosa L.) is a traditional plant that has antibacterial activity. The purpose of this study was to determine the antibacterial activity of the ethanol extract of Kencana Ungu leaves (Ruellia tuberosa L.) using TLC-Bioautography and Agar Diffusion methods. Simplisia from Kencana Ungu leaves (Ruellia tuberosa L.) was extracted by maceration method and then evaporated to obtain a thick extract. Preliminary testing, namely the antibacterial screening test, active extract at a concentration of 0.5% against Escherichia coli, Shigella dysenteriae, and Salmonella thypi. T

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Wardani, Erlita Kusuma, Evi Kurniawaty, and Oktadoni Saputra. "UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK RIMPANG KUNYIT Curcuma domestica TERHADAP BAKTERI Escherichia coli DAN Shigella dysenteriae." Jurnal Ilmu Kedokteran dan Kesehatan 10, no. 2 (2023): 1494–502. http://dx.doi.org/10.33024/jikk.v10i2.9056.

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Kunyit merupakan salah satu tanaman obat keluarga atau TOGA yang sedang menjadi perhatian banyak kalangan terutama di wilayah tropis seperti di negara Indonesia. Kunyit juga telah banyak diketahui fungsinya, di bidang kesehatan kunyit juga memiliki khasiat dalam mengatasi diare atau memiliki efek antibakteri terhadap bakteri-bakteri yang dapat menyebabkan diare. Rimpang kunyit (Curcuma domestica) memiliki senyawa antibakteri yaitu terpenoid, saponin, dan tanin. Penelitian ini dilakukan untuk menguji efek antibakteri ekstrak rimpang kunyit (Curcuma domestica) terhadap bakteri Escherichia coli d

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Schultz, C. "Isoenzyme analysis of typhoid-Shigella and Escherichia-Shigella hybrid vaccines and their parental strains." Journal of Clinical Microbiology 27, no. 12 (1989): 2838–41. http://dx.doi.org/10.1128/jcm.27.12.2838-2841.1989.

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Rezwan, Ferdousi, Ruiting Lan, and Peter R. Reeves. "Molecular Basis of the Indole-Negative Reaction in Shigella Strains: Extensive Damages to the tna Operon by Insertion Sequences." Journal of Bacteriology 186, no. 21 (2004): 7460–65. http://dx.doi.org/10.1128/jb.186.21.7460-7465.2004.

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ABSTRACT The molecular basis of the loss of tryptophan utilization (indole-negative phenotype) of Shigella strains, in effect clones of Escherichia coli, was investigated. Analysis of the tna operon of 23 Shigella strains representing each of the indole-negative serotypes revealed that insertion sequence-mediated insertion and/or deletions damaged the tna operon, leading to inability to convert tryptophan to indole. These events differ for cluster 1, cluster 3, and the outlier Shigella strains, confirming our previous observation of independent origins of these lineages from within E. coli. Pa

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Okeke, Iruka N., Aaron O. Aboderin, and Japheth A. Opintan. "Enteroinvasive Escherichia coli May Account for Uncultured Shigella." American Journal of Tropical Medicine and Hygiene 94, no. 2 (2016): 480–81. http://dx.doi.org/10.4269/ajtmh.15-0777a.

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OMURA, Kanzo, Akio YOSHIDA, Norihiko SUZUKI, et al. "Rapid Differentiation Method for Shigella and Escherichia coli." Journal of the Japanese Association for Infectious Diseases 66, no. 4 (1992): 456–64. http://dx.doi.org/10.11150/kansenshogakuzasshi1970.66.456.

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Lan, Ruiting, and Peter R. Reeves. "Escherichia coli in disguise: molecular origins of Shigella." Microbes and Infection 4, no. 11 (2002): 1125–32. http://dx.doi.org/10.1016/s1286-4579(02)01637-4.

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Thapa, Krishtina, and Sonu Shrestha. "Microbiological Analysis of Ice-cream Sold in Kathmandu Valley." Journal of Food Science and Technology Nepal 7 (June 16, 2014): 90–92. http://dx.doi.org/10.3126/jfstn.v7i0.10615.

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Different types of ice cream samples were collected from three different local vendors of Kathmandu (i.e. Samakhusi, Jawalakhel and Chabahil) for the microbial examination. Study explored that the ice cream samples from Chabahil showed the presence of highest number of mesophilic microorganisms with an average of 2.16×107 CFU/ml and 27.4 CFU/100ml of Coliform followed by samples collected from Jawalakhel and Samakhusi. The samples have been found to be contaminated with Coliforms (Enterobacter spp., Escherichia spp., Klebsiella spp., Citrobacter spp.), Salmonella (Salmonella typhi, Salmonella

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Hameed, Kanwal, Asif Ali Amir Ali, Sughra Parveen, Imran Khan, Jehangir Ali, and Abdul Waheed. "COMMON BACTERIOLOGICAL PATHOGENS AND THEIR ANTIBIOTIC SENSITIVITY PATTERN IN BILE OF PATIENTS WITH CHOLELITHIASIS." Infectious Diseases Journal of Pakistan 32, no. 1 (2023): 11–15. http://dx.doi.org/10.61529/idjp.v32i1.121.

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Background: To determine the pattern of antibiotic susceptibility of common bacteriological pathogens of bile in patients with Cholelithiasis. Material and Methods: A prospective cross-sectional study was conducted in the department of surgery, Jinnah Postgraduate Medical Center, from January 2020 to July 2020. All patients between the ages of 16 and 65, with symptomatic cholelithiasis operated by open or laparoscopic cholecystectomy were included in this study. Patients with acute cholecystitis, obstructive jaundice, and common bile duct stones were excluded from the study. Cultures and Sensi

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Zeng, Qinghuang, Renhe Zeng, and Jianbin Ye. "Alteration of the oral and gut microbiota in patients with Kawasaki disease." PeerJ 11 (July 10, 2023): e15662. http://dx.doi.org/10.7717/peerj.15662.

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Background Kawasaki disease (KD) is a multi-systemic vasculitis that primarily affects children and has an unknown cause. Although an increasing number of studies linking the gut microbiota with KD, the unchallengeable etiology of KD is not available. Methods Here, we obtained fecal and oral samples from KD patients and healthy controls, and then we use high-throughput sequencing to examine the diversity and composition of microbiota. Results Results showed that both in the gut and oral microbiota, the diversity of KD patients was significantly lower than that of the healthy controls. In the g

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Li, Yayue, Boyang Cao, Bin Liu, et al. "Molecular detection of all 34 distinct O-antigen forms of Shigella." Journal of Medical Microbiology 58, no. 1 (2009): 69–81. http://dx.doi.org/10.1099/jmm.0.000794-0.

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Shigella is the cause of shigellosis or bacillary dysentery, the occurrence of which is estimated to be 165 million cases per year worldwide, resulting in 1.1 million deaths. Rapid and reliable assays for detecting and identifying Shigella in food, environmental and clinical samples are therefore necessary. Shigella species are traditionally identified by their O antigens. This study developed a DNA microarray targeting O-serotype-specific genes to detect all 34 distinct O-antigen forms of Shigella, including Shigella boydii types 1–18, Shigella dysenteriae types 1–13, Shigella flexneri types

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LIN, WEN S., CHORNG-MING CHENG, and KHANH T. VAN. "A Quantitative PCR Assay for Rapid Detection of Shigella Species in Fresh Produce." Journal of Food Protection 73, no. 2 (2010): 221–33. http://dx.doi.org/10.4315/0362-028x-73.2.221.

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A quantitative PCR (qPCR) assay with two primers and a TaqMan probe targeting conserved regions of the specific ipaH gene of Shigella species and enteroinvasive Escherichia coli (EIEC) were developed. This qPCR assay was used to identify 206 Shigella strains (including four Shigella species with all serotypes and two provisional Shigella species), 3 EIEC strains, and 113 non-Shigella strains with 100% accuracy. Pure cultures of six Shigella reference strains were used to derive standard curves to determine the detection limit and efficiency of the qPCR method. The ipaH qPCR assay had an equall

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GOKALP, H. Y., H. YETIM, M. KAYA, and H. W. OCKERMAN. "Saprophytic and Pathogenic Bacteria Levels in Turkish Soud-jouks Manufactured in Erzurum, Turkey1." Journal of Food Protection 51, no. 2 (1988): 121–25. http://dx.doi.org/10.4315/0362-028x-51.2.121.

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In Turkey, spicy, typically dry, fermented sausage (soudjouk) is one of the most popular processed meat products. In this study, 42 soudjouk samples were collected from the eight manufacturers in Erzurum, Turkey. These samples were evaluated for aerobic plate count (APC) at 37 and 25°C, psychrotrophic, coliform, Escherichia coli, and coagulase-positive Staphylococcus aureus counts and presence of Salmonella and Shigella spp. Generally, all the samples had very high counts of most of the bacteria enumerated. In two samples of the 42, Shigella spp. was found and one of them was Shigella boydii.

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BIN KINGOMBE, CESAR I., MARIA-LUCIA CERQUEIRA-CAMPOS, and JEFFREY M. FARBER. "Molecular Strategies for the Detection, Identification, and Differentiation between Enteroinvasive Escherichia coli and Shigella spp." Journal of Food Protection 68, no. 2 (2005): 239–45. http://dx.doi.org/10.4315/0362-028x-68.2.239.

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A strategy for the detection, identification, and differentiation of enteroinvasive Escherichia coli (EIEC) and Shigella spp. has been developed. The strategy includes (i) a multiplex PCR for the amplification of two virulence genes, i.e., iuc (222 bp) and ipaH (629 bp); (ii) amplification of the ial gene (a 1,038-bp amplicon) located within a large plasmid; and (iii) restriction fragment length polymorphism (RFLP) of the ial gene amplicon. The multiplex PCR provided three patterns. Pattern 1 (iuc−/ipaH+) was found in 10 (67%) of 15 EIEC strains tested, pattern 2 (iuc+/ipaH−) in only 2 (4.4%)

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Gorrasi, Susanna, Marcella Pasqualetti, Andrea Franzetti, et al. "Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone." Water 13, no. 11 (2021): 1443. http://dx.doi.org/10.3390/w13111443.

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Enterobacteriaceae is present in various niches worldwide (i.e., the gastrointestinal tracts of animals, clinical specimens, and diverse environments) and hosts some well-known pathogens (i.e., salmonellas, shigellas and pathogenic coliforms). No investigation has focused on its occurrence in marine salterns, and it is not clear if these hypersaline environments could be a reservoir for these bacteria including some potentially harmful members. In this study, a two-year metabarcoding survey was carried out on samples collected from different ponds of the “Saline di Tarquinia” salterns and the

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Junaedi, Fhadillah, Rachmat Kosman, and Herwin Herwin. "IDENTIFICATION OF ACTIVE CHEMICAL COMPONENTS OF ETHANOL EXTRACT OF PAINTED NETTLE LEAVES (COLEUS SCUTELLARIOIDES L. BENTH) AGAINST GASTROINTESTINAL INFECTION BACTERIA USING TLCBIOAUTOGRAPHY AND AGAR DIFFUSION METHOD." Journal Microbiology Science 3, no. 1 (2023): 1–12. http://dx.doi.org/10.56711/jms.v3i1.869.

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Infectious diseases are one of the public health problems caused by the transmission and multiplication of microorganisms. The research on the antibacterial activity of painted nettle leaves (Coleus scutellarioides L. Benth) extracts was carriedout to examine the activity and chemical components that provide inhibition against gastrointestinal infection bacteria. The extraction of painted nettles leaves performed maceration methd using a solvent of 96%. The extraction results were examined to determine the antibacterial activity at 1% concentration of the test bacteria: Escherichia coli, Salmo

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Chowdhury, Fatema Moni, Sirajul Islam Khan, Nils Kåre Birkeland, and Chowdhury Rafiqul Ahsan. "Antigenic Cross-Reactivity Between Escherichia albertii DM104 and Different Shigella spp." Bangladesh Journal of Microbiology 35, no. 1 (2019): 17–21. http://dx.doi.org/10.3329/bjm.v35i1.39799.

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Environmental Escherichia albertii strain DM104 has been found to induce protective immunity against Shigella dysenteriae in guinea pig model and intranasal immunization showed promising results in terms of antibody response and protective efficacy. For selecting a proper immunodiagnostics marker against shigellosis, the current study investigated the antigenic cross-reactivity between DM104 and four different Shigella spp (S. dysenteriae type 4, S. flexneri type 2a, S. boydii type 15 and S. sonnei). At least six antigenic protein bands (85, 72, 34, 30, 23 and 20 k Da) of the surface component

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Harutyunyan, Shushan, Irene Neuhauser, Alexandra Mayer, et al. "Characterization of ShigETEC, a Novel Live Attenuated Combined Vaccine against Shigellae and ETEC." Vaccines 8, no. 4 (2020): 689. http://dx.doi.org/10.3390/vaccines8040689.

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Background: Shigella spp. and enterotoxigenic Escherichia coli (ETEC) remain the two leading bacterial causes of diarrheal diseases worldwide. Attempts to develop preventive vaccines against Shigella and ETEC have not yet been successful. The major challenge for a broad Shigella vaccine is the serotype-specific immune response to the otherwise protective LPS O-antigen. ETEC vaccines mainly rely on the heat-labile enterotoxin (LT), while heat-stable toxin (ST) has also been shown to be an important virulence factor. Methods: We constructed a combined Shigella and ETEC vaccine (ShigETEC) based o

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ESTUNINGSIH, SRI, CLAUDIA KRESS, ABDULWAHED A. HASSAN, ÖMER AKINEDEN, ELISABETH SCHNEIDER, and EWALD USLEBER. "Enterobacteriaceae in Dehydrated Powdered Infant Formula Manufactured in Indonesia and Malaysia." Journal of Food Protection 69, no. 12 (2006): 3013–17. http://dx.doi.org/10.4315/0362-028x-69.12.3013.

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To determine the occurrence of Salmonella and Shigella in infant formula from Southeast Asia, 74 packages of dehydrated powdered infant follow-on formula (recommended age, &gt;4 months) from five different manufacturers, four from Indonesia and one from Malaysia, were analyzed. None of the 25-g test portions yielded Salmonella or Shigella. However, further identification of colonies growing on selective media used for Salmonella and Shigella detection revealed the frequent occurrence of several other Enterobacteriaceae species. A total of 35 samples (47%) were positive for Enterobacteriace

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