Journal articles: 'Escherichiosis'

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Tishchenko, Alexander, Vladimir Terekhov, Nikolay Pimenov, and Regina Ivannikova. "Prevention of enterotoxigenic escherichiosis as a way to stabilize the ecological situation of anthropogenic pollution by antibiotic-resistant strains." IOP Conference Series: Earth and Environmental Science 981, no. 4 (February 1, 2022): 042067. http://dx.doi.org/10.1088/1755-1315/981/4/042067.

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Abstract The ecological niches occupied by E. coli are not limited to the environment, this bacterium is part of the symbiotic and conditionally pathogenic microflora of humans and animals. E. coli strains are highly resistant to various antibiotics, which poses a threat to ecological stability in the biodiversity of species. Once in unfavorable conditions, E. coli can acquire pathogenic properties and cause dangerous diseases, one of which is enterotoxigenic escherichiosis. The reservoir of pathogenic E. coli in the environment can be both farm animals and animal products. Timely and effective immunization of animals against escherichiosis is relevant for veterinary medicine and medicine, and the search for new biological products for treatment and prevention is one of the important practical tasks. The preventive effectiveness of the use of anatoxin vaccine samples in combination with an adjuvant complex in enterotoxigenic escherichiosis of animals was studied as a new solution to stabilize the ecological situation of anthropospheric genesis in the form of reducing the spread of antibiotic-resistant strains of E. coli in the environment. As a result of the conducted studies, it was established that after the use of an anatoxin vaccine with an adjuvant complex, high preventive efficacy is provided for enterotoxigenic escherichiosis.

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Shaimukhametov, M. A., and A. I. Ivanov. "DYNAMICS AND THERAPEUTIC MEASURES FOR COLIBACTERIOSIS OF YOUNG CATTLE." RUSSIAN ELECTRONIC SCIENTIFIC JOURNAL 38, no. 4 (December 19, 2020): 29–39. http://dx.doi.org/10.31563/2308-9644-2020-38-4-29-35.

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Gastrointestinal diseases of farm animals, including Escherichiosis of young cattle, occupy one of the leading places in frequency, mass manifestation and magnitude of damage to farms and mortality of newborn young animals, as well as high costs of medical measures. The incidence of escherichiosis and its spread among young cattle depends not only on the source of infection and susceptible calves, but also on a number of other factors, such as physical inactivity, keeping adult animals with young in the same room, non-observance of the principles of keeping “everything is empty, everything is busy”. Escherichiosis manifests itself mainly in the form of diarrhea. The article presents the results of studies on the dynamics of the incidence of colibacillosis in young cattle from 2004 to 2018, as well as materials on the implementation of therapeutic measures for two groups of calves.

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Pirozhkov, Mikhail K., Alsu A. Galiakbarova, and Nikolay V. Pimenov. "The current state of the domestic market for vaccines against colibacillosis of animals." Veterinariya, Zootekhniya i Biotekhnologiya 2, no. 99 (2022): 12–20. http://dx.doi.org/10.36871/vet.zoo.bio.202202002.

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Epizootic well-being and the effectiveness of preventive work against colibacillosis (escherichiosis), first of all, are provided by specific immunoprophylaxis. Effective vaccination is an important factor in preserving young stock and productivity of farm animals. The paper presents a brief description of the disease and the history of the development of vaccines against colibacillosis, and also analyzes the possibilities of using domestic and foreign biological products registered for the specific prevention of escherichiosis in the Russian Federation. An assessment of the current state of the vaccine market in our country is given.

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Shaimukhametov, Marat, and Aleksandr Ivanov. "EPIZOOTOLOGY AND ETIOLOGY OF ESCHERICHIOSIS IN CALVES." Russian Electronic Scientific Journal 29, no. 3 (2018): 228–35. http://dx.doi.org/10.31563/2308-9644-2018-29-3-228-235.

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Buribayeve, B. I., I. A. Kasimov, and Z. T. Xalilova. "SOME CLINICAL ASPECTS AND COMPARATIVE CHARACTERISTICS OF ENTEROPATHOGENIC AND ENTEROINVASIVE ESCHERICHIA COLIINFECTIONIN CHILDREN." UZBEK MEDICAL JOURNAL Special issue, no. 1 (March 30, 2021): 16–21. http://dx.doi.org/10.26739/2181-0664-2021-si-1-3.

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The clinical course of enteropathogenic (EPEC) and enteroinvasive escherichiosis (EIEC) in children was studied and their comparative characteristics were carried out. We examined 82 sick children with early age Escherichiosis, caused in 28 byenteroinvasive, in 54 with enteropathogenic strains. In children with EPEC, secretory diarrhea was observed, and EIEC —colitis. A more pronounced manifestation of intoxication syndrome was observed in children with EPEC. The manifestations of diarrheal syndrome and electrolyte disturbances, assessed by the degree of dehydration, are more typical for children with EPEC. If in children with EPEC such complications as hypovolemic shock and acute renal failure were encountered, then in children with EIEC in the form of complications of infectious toxic shock, neurotoxicosis and disseminated intravascular coagulation.

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Buribayeve, B. I., I. A. Kasimov, and Z. T. Xalilova. "SOME CLINICAL ASPECTS AND COMPARATIVE CHARACTERISTICS OF ENTEROPATHOGENIC AND ENTEROINVASIVE ESCHERICHIA COLIINFECTIONIN CHILDREN." UZBEK MEDICAL JOURNAL Special issue, no. 1 (March 30, 2021): 16–21. http://dx.doi.org/10.26739/2181-0664-2021-si-1-3.

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The clinical course of enteropathogenic (EPEC) and enteroinvasive escherichiosis (EIEC) in children was studied and their comparative characteristics were carried out. We examined 82 sick children with early age Escherichiosis, caused in 28 byenteroinvasive, in 54 with enteropathogenic strains. In children with EPEC, secretory diarrhea was observed, and EIEC —colitis. A more pronounced manifestation of intoxication syndrome was observed in children with EPEC. The manifestations of diarrheal syndrome and electrolyte disturbances, assessed by the degree of dehydration, are more typical for children with EPEC. If in children with EPEC such complications as hypovolemic shock and acute renal failure were encountered, then in children with EIEC in the form of complications of infectious toxic shock, neurotoxicosis and disseminated intravascular coagulation.

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Barkhina, T. G., A. �. Ali-Riza, and Yu G. Parkhomenko. "Submicroscopic features of cecal cells in experimental escherichiosis." Bulletin of Experimental Biology and Medicine 114, no. 3 (September 1992): 1333–38. http://dx.doi.org/10.1007/bf00809572.

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Gonchar, N. V., O. I. Klimova, I. V. Razd'yakonova, A. V. Orlov, and A. S. Kvetnaya. "Clinical case of combined intestinal infection caused by enteroaggregative Escherichia coli and toxigenic strain Clostridium difficile in a child with cystic fibrosis." Journal Infectology 13, no. 3 (October 9, 2021): 143–49. http://dx.doi.org/10.22625/2072-6732-2021-13-3-143-149.

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The goal is to study the peculiarities of the clinical picture and the distant outcome of intestinal infection due to enteroaggregative escherichiosis and the toxigenic strain of C. difficile in a child with cystic fibrosis.Material and methods. To verify the etiology of intestinal infection, the following studies were conducted. — fecal PCR “OKI-screen” tests to detect viral and bacterial pathogens, fecal bacteriopsy for pathogenic and opportunistic microbes; determination of C. difficile A and B toxins in feces by enzyme-linked fluorescence analysis.Results. The combined intestinal infection caused by en-teroaggregative escherichiosis and a toxigenic strain of C. difficile, in a child with pulmonary-intestinal form of cystic fibrosis was characterized by a wavy course, a pronounced intoxication syndrome, excicosis, hemorrhagic enterocolitis, signs of systemic and local inflammation, metabolic disorders. There were no recurrences of C. difficile-infection in catamnese.Conclusion. It is necessary to continue research on the course of intestinal infections caused by bacterial associations of pathogens.

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Ali-Riza, A. �., Yu G. Parkhomenko, and T. G. Barkhina. "Morphology and function of the cecum in experimental escherichiosis." Bulletin of Experimental Biology and Medicine 114, no. 4 (October 1992): 1565–68. http://dx.doi.org/10.1007/bf00841619.

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10

Olkhovskyi, Yevhen. "Immune response of escherichiosis infected children with epstein-barr virus." ScienceRise: Medical Science, no. 2 (22) (February 27, 2018): 19–24. http://dx.doi.org/10.15587/2519-4798.2018.124273.

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Syrota, B. V. "Patogenetic value of intestine microbiota ecosystem in the escherichiosis enterocolitis development." Bukovinian Medical Herald 21, no. 1 (81) (February 23, 2017): 141–45. http://dx.doi.org/10.24061/2413-0737.xxi.1.81.2017.29.

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Akchurin, Sergey Vladimirovich, and Irina Vladimirovna Akchurina. "Analysis of proportion of nucleic acids and proteins in glandular stomach wall in chickens during experimental escherichiosis." Agrarian Scientific Journal, no. 4 (April 22, 2020): 44–50. http://dx.doi.org/10.28983/asj.y2020i4pp44-50.

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A method for spectral analysis of the ratios of organic substances in a cell using the "Stains all" metachromatic luminescent dye (in its own modification) has been developed. This method is used to establish the ratio of nucleic acids and proteins in the integumentary epithelium of the mucous membrane and in the interlobular connective tissue of the submucosa tissue of the glandular stomach of chickens. Using the developed biophysical method, we studied the ratios of these organic substances in the glandular stomach of healthy and infected with E. coli chickens. The dynamics of the ratios of nucleic acids and proteins detected in the wall of the glandular stomach of the control group chicks fits into the picture of a moderate and uniform increase in the values of these indicators, corresponding to an age increase of the chickens. In chickens affected by Escherichiosis, a curve that reflects the dynamics of the ratios includes a three-fold increase in their values. The results obtained using the developed method indicate the possibility of using it to detect early metabolic changes in the glandular stomach of chickens before the appearance of a characteristic pathomorphological and clinical picture. Thus, this method can provide invaluable assistance in developing a fundamentally new approach in creation of modern technologies for the diagnosis, prevention and treatment of widespread disease Escherichiosis.

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Olkhovskyy, Ye S., and S. V. Kuznetsov. "Improvement of therapy for escherichiosis in children infected with Epstein-Barr virus." CHILD`S HEALTH 12, no. 2.1 (June 6, 2017): 292–96. http://dx.doi.org/10.22141/2224-0551.12.2.1.2017.100997.

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14

Tishchenko, Alexander, Vladimir Terekhov, and Buabeng Emanuel. "The effects of inactive toxins of escherichia coli on hematological parameters in animals." IOP Conference Series: Earth and Environmental Science 839, no. 5 (September 1, 2021): 052003. http://dx.doi.org/10.1088/1755-1315/839/5/052003.

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Abstract Escherichiosis of calves and piglets is still a widespread infectious pathology on farms in different countries, including Russia, despite the vaccine prophylaxis of this disease. This fact testifies to the imperfection of present immunization means, the cause of which is discrepancy between antigenic composition of vaccines and etiological and pathogenetic factors responsible for the development of escherichiosis. In view of the above, the question of joint application of enterotoxins of Escherichia coli as a complex vaccine (anatoxin) becomes relevant. The question remains as to how they will affect the animal body after vaccine’s administration and what properties they will have, requiring special studies. In this regard, the aim of the work was to study the effect of the mixture of inactivated enterotoxins on the leukogram of animals. As a result of studies, it was found that after the introduction of anatoxin in rats, the primary response of the immune system was expressed in the form of an increase in the quantitative presence of physiologically mature neutrophils, followed by an increase in the number of immunocompetent cells – lymphocytes. The dose of injected anatoxin also mattered: the higher was the dose, the more pronounced were the changes in the leukogram, including the changes manifested by the increased presence of eosinophils in the bloodstream. Inactivated E. coli enterotoxins had no toxic and cytopathological effects when injected into the macroorganism; at the same time, they retained their antigenic and immunostimulatory properties, which allows them to be considered a candidate for a complex vaccine.

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15

Labazava, I. E., E. I. Kozeltsava, and E. A. Piatrova. "QUALITY ASSESMENT OF MEAT PRODUCT BY MICROBIOLOGICAL INDICATORS." Food Industry: Science and Technology 13, no. 4(50) (June 15, 2020): 95–102. http://dx.doi.org/10.47612/2073-4794-2020-13-4(50)-95-102.

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The meat industry takes a leading place among all branches of the food industry. At the same time, meat and its processed products are a serious factor in the spread and increased risk of listeriosis, salmonellosis, campylobacteriosis, botulism, poisoning with staphylococcal enterotoxin, escherichiosis, protozoa. One of the mandatory requirements for the quality of food products is their safety for human health and stability during storage and sale. Of particular importance to the consumer is the microbiological safety of food products, the provision of which is the main task of bacteriological control at enterprises producing meat and meat products

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16

Barkhina, T. G., A. �. Ali-Riza, and Yu G. Parkhomenko. "Ultrastructure of cecal endocrine cells in normal mice and mice with experimental escherichiosis." Bulletin of Experimental Biology and Medicine 114, no. 4 (October 1992): 1543–47. http://dx.doi.org/10.1007/bf00841614.

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17

Tyshkivska, N., V. Lyasota, A. Tyshkivska, N. Bukalova, and N. Bogatko. "Monitoring and diagnosis of poultry bacterial diseases in poultry farms of the Kyiv region." Naukovij vìsnik veterinarnoï medicini, no. 1(154) (May 21, 2020): 47–53. http://dx.doi.org/10.33245/2310-4902-2020-154-1-47-53.

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Bacterial safety in the modern poultry industry plays a key role and is one of the key factors in production eﬃ ciency. In the structure of poultry infectious pathology, the leading place is occupied by such bacteria as Escherichia coli, Clostridium perfringens, Enterococcus cecorum, Staphylococcus aureus, Gallibacterium anatis. The results of studies of samples of pathological material obtained from a sick bird are presented that indicate that most often Escherichia coli cultures were isolated from the heart (41,5 %), liver (22,0 %) and lungs (20,7 %), less often spleen (5,2 %) and kidney (2,0 %). Most isolated cultures of Escherichia coli (78 %) caused hemolysis when plating material on blood agar. The largest number of pathogenic cultures of Escherichia coli was isolated from adult chickens, signiﬁ cantly fewer chickens under the age of 20 days. The associated course of bacterioses caused by two or more pathogens was noted in 89,8 % of cases. In 38,5 % of cases, Escherichia coli, bacteria of the genus Staphylococcus and Gallibacterium anatis were isolated from pathological material, in 27,3 % – a joint course of escherichiosis, staphylococcosis and enterobacteriosis was noted, in 15,7 % – escherichiosis, salmonellosis and enterobacteriosis, in 8,3 % – pasteurellosis and enterobacteriosis. In 13,2 % of cases with pathological material from chickens (liver, joints, in chickens – blind processes of the intestine) Clostridium perfringens was isolated. Three species of bacteria of the genus Staphylococcus were identiﬁ ed: Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus pluranimalium (51,7 % from the liver, 21,7 from the lungs, 18,3 from the spleen, 5,0 % from the kidneys). Enterococcus cecorum was identiﬁ ed in 11,32 % of the studied samples from cloacal swabs, oviducts and bone marrow, and Gallibacterium anatis was identiﬁ ed in 11,32 % (from the upper respiratory tract and genitals). Key words: poultry farms, monitoring, bacterial infection, Escherichia coli, Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus pluranimalium, Enterococcus cecorum, Clostridium perfringens, Gallibacterium anatis.

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Syrota, B. V. "Immuno-hematological indices of endogenous intoxication and cellular reactivity at acute intoxication diarrheal escherichiosis." Bukovinian Medical Herald 23, no. 1 (89) (March 30, 2019): 74–80. http://dx.doi.org/10.24061/2413-0737.xxiii.1.89.2019.11.

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Gryazneva, T. N., D. G. Reshetnikova, and S. Yu Karabanov. "Experience in conducting veterinary-sanitary and zoohygienic surveys of farms that are unfavorable for bacterial infections of cattle." Veterinariya, Zootekhniya i Biotekhnologiya 1, no. 1 (2021): 41–47. http://dx.doi.org/10.36871/vet.zoo.bio.202101005.

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The article presents the results of veterinary-sanitary and zoohygienic examination of the farm that is unfavorable for bacterial infections-pasteurellosis, moraxellosis, escherichiosis, and other pathologies. There is a high incidence of mastitis and endometritis in cows, and gastrointestinal and respiratory diseases in young animals. 32,6 «marker points» of violations of conditions of keeping, feeding and exploitation of animals were identified. Recommendations are given for the elimination of violations, after which the incidence of respiratory diseases in calves decreased by 48,2%, gastrointestinal diseases – by 93,0%, the manifestation of infectious keratoconjunctivitis caused by moracella decreased by 12,0%, and the incidence of chronic endometritis in cows decreased by XNUMX%. The farm was recovered from clinical, subclinical mastitis and postpartum endometritis.

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Yulia, Kozak, Seregin Ivan, and Zabolotnykh Mikhail. "Poultry meat biological value and safety after poultry forced slaughter." Poultry and Chicken Products 24, no. 1 (2022): 58–60. http://dx.doi.org/10.30975/2073-4999-2022-24-1-58-60.

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The results have been presented in the paper for chemical composition, biological value and safety of poultry meat after poultry culling and forced slaughter due to some infectious or non-infectious diseases comparing to healthy stock qualities. Deviations has been studied in chemical composition and biological value of poultry meat in such the most common diseases as pseudomonosis, salmonellosis and escherichiosis, and also in peritonitis, alimentary dystrophy and pecking. It was established that deviations have place for the worse for chemical composition, biological value and safety traits in veterinary culling of ill poultry. Some suggestions have been developed at the base of data received for veterinary and sanitary poultry meat expertise after poultry forced slaughtering.

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Spiridonov et al., Gennadiy N., Andrey I. Nikitin, Haris N. Makaev, Konstantin Kh Papunidi, Albert N. Chernov, Gulnara H. Murtazina, and Anton G. Spiridonov. "The development of preparations for specific prevention and treatment of anaerobic enterotoxemia and escherichiosis in calves." Bali Medical Journal 6, no. 2 (May 11, 2017): 363. http://dx.doi.org/10.15562/bmj.v6i2.520.

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Tul, O. I. "Pathomorphological changes in the sand lizard (Lacerta agilis) organism during the associated course of escherichiosis and staphylococcosis." Veterinary science, technologies of animal husbandry and nature management, no. 5 (2020): 192–97. http://dx.doi.org/10.31890/vttp.2020.05.34.

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Веприкова (Veprikova), Евгения (Evgenija) Владимировна (Vladimirovna), Светлана (Svetlana) Алексеевна (Alekseevna) Кузнецова (Kuznecova), Ирина (Irina) Владимировна (Vladimirovna) Королькова (Korol'kova), Анастасия (Anastasija) Анатольевна (Anatol'evna) Мороз (Moroz), Светлана (Svetlana) Анатольевна (Anatol'evna) Счисленко (Schislenko), Борис (Boris) Николаевич (Nikolaevich) Кузнецов (Kuznecov), and Николай (Nikolaj) Васильевич (Vasil'evich) Чесноков (Chesnokov). "INVESTIGATION OF SORPTION AND THERAPY-PROPHYLACTICS PROPERTIES OF THE ENTEROSORBENT FROM A LARCH BARK." chemistry of plant raw material, no. 1 (October 8, 2017): 201–9. http://dx.doi.org/10.14258/jcprm.2018012680.

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The sorption capacity of enterosorbent from a larch bark for marker substances modeling the different toxins kinds - methylene blue and gelatine – was investigated. Influence of values pH of model solutions and background electrolyte (0,9 mass % NaCl) addition on the methylene blue and gelatine sorption by enterosorben were investigated. It was shown that designed enterosorbent are able sorb the marker substances from solutions modeling of the stomach and intestine environment conditions. Investigated enterosorbent show the highest efficiency at model conditions of intestine environment. It was shown that enterosorbent from a larch bark exceed commercial enterosorbents on based of hydrolyzed lignin «Polyphepan» in its capacity to sorb the marker substances. On the biological models was determined that the designed enterosorbent show the therapy-prophylactics properties to gastrointestinal infections - escherichiosis. As a result of the bacteriological investigations was determined that the quantity of pathogenic culture E.coli increased 50 times at enterosorbent using in the experimental therapy group, in the prophylactics therapy group – 25 times. The obtained results indicate that the designed enterosorbent from a larch bark have good perspectives for application in veterinary practice.

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Svetlana A., Kuznetsova, Skvortsova Galina P., Moroz Anastasya A., Korolkova Irina V., Schislenko Svetlana A., Levdansky Vladimir A., Kuznetsov Boris N., and Chesnokov Nikolai V. "Obtaining Veterinary Preparations from Aspen Bark and the Study of their Therapeutic and Prophylactic Properties in Experimental Escherichiosis of Animals." Journal of Siberian Federal University. Chemistry 11, no. 4 (December 2018): 604–15. http://dx.doi.org/10.17516/1998-2836-0103.

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Frolov, A. V., G. I. Rakhmatullina, V. A. Guryanova, E. N. Mayorova, and R. A. Volkov. "ASSESSMENT OF ANTITOXIC AND ANTI-INFECTIOUS ACTIONS OF THE EXPERIMENTAL PREPARATION." Scientific Notes Kazan Bauman State Academy of Veterinary Medicine 248, no. 4 (December 1, 2021): 246–49. http://dx.doi.org/10.31588/2413-4201-1883-248-4-246-249.

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In the course of the research, the antitoxic and anti-infectious effects of the developed drug were determined on white mice and white rats. To assess the antitoxic effect in the experiment on white rats, cadmium damage was modeled, followed by taking into account the antidote activity. An increase in the survival rate of animals receiving the drug was found – 60 % versus 40 % in the control, higher hematological indicators: the content of hemoglobin, erythrocytes and leukocytes in the blood of experimental animals was higher than similar control values, respectively, by 3.8, 15.3 and 16,4 %. The anti-infectious effect of the feed additive was determined during the experiment on infecting white mice with the causative agent of Escherichiosis – E. coli (strain "KB-1"). It was found that the introduction of the study drug into the diet of infected animals smoothed the clinical picture of the infectious process, had a hemoprotective effect: the content of erythrocytes, leukocytes, neutrophils, lymphocytes, neutrophils in the blood of experimental animals exceeded the control values, respectively, by 2.5, 13.6, 16, 7, 14.3, 28.6 %. The results of the investigated subject showed that the investigational drug has a well-pronounced antitoxic and anti-infectious effect.

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Kapustin, A. V., A. I. Laishevtcev, T. I. Aliper, O. A. Verkhovskiy, A. P. Kotelnikov, A. M. Mishin, K. Y. Kunakov, and E. V. Shemelkov. "THE RESULTS OF CLINICAL STUDIES OF SAFETY, ANTIGENIC ACTIVITY AND EFFECTIVENESS OF INACTIVATED VACCINE «VERRES-KOLIKLOST» AGAINST ESCHERICHIOSIS AND CLOSTRIDIOSIS OF PIGS." Russian Journal of Agricultural and Socio-Economic Sciences 66, no. 6 (June 30, 2017): 352–60. http://dx.doi.org/10.18551/rjoas.2017-06.42.

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Balnikov, A., N. Kostomakhin, E. Gridyushko, I. Gridyushko, V. Ruzuvanova, and V. Tsaruk. "The evaluation of productive traits of pigs with application methods of population selection and molecular genetic markers." Glavnyj zootehnik (Head of Animal Breeding), no. 7 (July 1, 2020): 36–49. http://dx.doi.org/10.33920/sel-03-2007-05.

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The comprehensive evaluation of the productive traits of the initial genotypes of pigs in the creation of breeding herds using population selection methods has been carried out. Based on the results of evaluating the growth and development of breeding animals using the methods of index selection and DNA testing for the genes markers RYR1, ESR, IGF-2, MUC4 the genealogical structure of breeding herds has been formed on the basis of new stud lines of Belarusian stud type of pigs of Yorkshire breed. It has been found that sows of new lines had high reproduction rates: prolifi cacy was 11,6 piglets, milk capacity was 59,1 kg, number of piglets at weaning was 11,2 heads, and litter weight at weaning was 103,6 kg. The breeding herd of two new stud lines is represented by boars of the line Drug 6805 and Dobry 2313. The average productivity of boars-line continuers was: the age of reaching a live weight of 100 kg to 160 days, the average daily gain from birth to reaching the live weight of 100 kg was 636 g. At the age of 12 months, the live weight and body length of replacement boars were 268 kg and 175 cm, the thickness of the fat was 8,9 mm, the height of the longest back muscle was 51,5 mm, the content of lean meat in the body was 62,6 %, which corresponds to the class “elite”. Boars have been separated into reproductive traits. Improvement of lines is carried out through the continuators of related groups. Analysis of genetic studies of animals of new stud lines has shown that they have a high frequency of occurrence of desirable alleles by genes: RYR1 (stress-resistant animals) – 1,0; IGF-2 (fattening and meat traits) – 0,783; ESR (reproductive traits) – 0,608; MUC4 – (resistance to escherichiosis) – 0,786.

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Klimova, O. I., N. V. Gonchar, I. V. Razd’yakonova, and Yu V. Lobzin. "Etiological and epidemiological characteristics of infectious hemocolitis in hospitalized pediatric patients." Journal Infectology 13, no. 1 (March 30, 2021): 86–92. http://dx.doi.org/10.22625/2072-6732-2021-13-1-86-92.

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The aim of this work is to study the etiological and epidemiological features of infectious hemocolites (IG) in hospitalized children of different ages.Materials and methods of research. An analysis of the results of a survey of 3103 children admitted to hospital treatment for acute intestinal infections (AII) from January to December 2018, among which patients with IG were identified. The etiology of the disease was determined based on the results of the bacteriological method, PCR studies of faeces with Amplicens ® OKI screen-FL reagents, serological and immunological methods. Microscopic examination of faeces was performed to identify protozoa. Patients were divided into age groups: infant (n=78; 30%); early (n=74; 28,5%); preschool (n=63; 24,2%), school (n=45; 17,3%).Results. The incidence of IG in hospitalized children with AII was 8,4%. Bacterial pathogens of IG were detected in 66,5% of children, IG of unspecified etiology was diagnosed in 24,2%. Viral and bacterial infections were rarely detected (9,2%). Among intestinal viruses, rotavirus (37,5%), norovirus (29,2%) and enterovirus (20,8%) were more frequently detected in hemocolitis of combined viral and bacterial etiology. In the IG age structure, infants (30%), young children (28,5%) and pre-school children (24,2%) made up the majority. The maximum number of IG patients was detected in the summer (10,9% of all cases of AII). Salmonellosis was more often detected in autumn (31,6%), campylobacteriosis – in summer (17.9%) and autumn (24,1%), escherichiosis and shigellosis – in summer (11,5% and 6,4%, respectively). The maximum detection of IG in infants was observed in June (14,1%) and October (12,8%), in young children – in July (17,6%), in preschoolers – in June (12,7%) and November (15,9%), in schoolchildren in May (13,3% of cases) and in October (15,6%).Conclusion. The incidence of infectious hemocolites in hospitalized children with acute intestinal infections was 8,4%. The bacterial etiology of the disease was detected in 66,5% of children. The age structure of the IG was dominated by children of infant, early and preschool age. The maximum detection of patients with IG was observed in the summer.

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Gonchar, N. V., S. G. Mardanly, K. D. Ermolenko, I. V. Razd’yakonova, T. A. Koroleva, E. A. Martens, V. S. Kovalev, and A. E. Maklakova. "Effectiveness and safety of «Nifuroxazide-ECO» in therapy of escherichioses in children." Journal Infectology 13, no. 1 (March 30, 2021): 71–77. http://dx.doi.org/10.22625/2072-6732-2021-13-1-71-77.

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In conditions of increasing antibiotic resistance and widespread antibiotic-associated diarrhea caused by Clostridium difficile, it is necessary to choose a rational drug for the empirical treatment of escherichioses, which has a wide range of antibacterial activity and does not lead to the development of serious side effects and disruption of the gut microbiota.The aim of the study is to assess the effectiveness and safety of the use of the drug “Nifuroxazid-ECO” in the integrated therapy of escherichioses in children.Patients and methods: 50 patients aged 1 month to 18 years were selected for work by random sampling, hospitalized in the intestinal department of the Pediatric Research and Clinical Center for Infectious Diseases of Russia with non-severe clinical forms of escherichioses. All patients underwent routine clinical, biochemical, instrumental examination. DNA detection of the pathogen was carried out by molecular methods in fecal samples using a set of reagents to detect and differentiate the DNA of diaregenic E. coli in environmental objects and clinical material by polymerase chain reaction (PCR) with hybridization-fluorescent detection. Patients received the drug “Nifuroxazid-ECO”at age dosage, every 6–8 hours for 5–7 days.Results: Against the background of the therapy, a significant majority of patients showed clinical improvement. The average duration of hospitalization was 4.8 days, the duration of preservation of diarrhoeal syndrome was 3.4 days. The drug showed good tolerability. There were no cases of antibiotic-associated diarrhea, re-hospitalization at an early date after treatment, serious adverse reactions.Conclusion: Studies have shown that the drug “Nifuroxazid- ECO” is a safe and effective modern drug which can be successfully used in the therapy of non-severe escherichioses in children.

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Sysa, L. "The eﬀect of dry blood plasma as a biological supplement on pigs." Naukovij vìsnik veterinarnoï medicini, no. 1(154) (May 21, 2020): 32–39. http://dx.doi.org/10.33245/2310-4902-2020-154-1-32-39.

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The effect of preparations from whole blood (dry plasma) on the animal organism was studied. In the course of our research, 2 groups of animals with 15 animals in each of 10 days of age were formed on the basis of analogues. The first group of animals was given dry plasma mixed with mixed feed (based on 5% of the feed weight), the second group was the control one and received no additives. Piglets of both groups were kept in the same sanitary and hygienic conditions, every day they evaluated the clinical status of animals, took into account morbidity, mortality, mortality, weighed, and blood was taken for morphological and biochemical blood tests. It was found that in the group of animals that used dry plasma, they gained weight more intensively during 1.5-2 months than the pigs of the control group, a low percentage of the incidence of pathologies from the gastrointestinal tract and respiratory system was established (2-4% whereas in the control group - 9-14%), higher weight gain (10-15%), mortality was not observed (whereas in the control group 2 pigs fell on the background escherichiosis and salmonellosis). Piglets of the experimental group were mobile, active, appetite expressed. In the control group, in addition to the above morbidity and mortality, low daily weight gain was observed (350 grams per day), some animals were inactive, lethargic, and a decrease in appetite was observed (50% of the animals of the group). The results of laboratory studies showed that the use of dry plasma in the diet of piglets contributes to the fastest restoration of blood counts to physiological norm. So already on the 15th day after giving dry plasma, the number of red blood cells was in the range 6.0 ± 0.42 x 1012 / l, platelets 180.5 ± 1.3 x 109 / l, the amount of hemoglobin in the range of 90.5 ± 0, 95 g / l, white blood cells 15.33 ± 0.62 x 109 / l, a decrease in ESR to 1.55 ± 0.07 mm / h was observed, the amount of total protein was in the range of 64.55 ± 2.12 g / l , the level of albumin is 18.89 ± 3.52 g / l, the activity of the enzymes AsAT, AlAT 0.55 ± 0.06 μkat / l and 0.66 ± 0.03 μkat / l, respectively, the activity of alkaline phosphatase was at level 1, 01 ± 0.12 μkat / l, bilirubin 7.51 ± 0.168 mmol / l, which indicates an improvement in metabolism, in the first The next step is protein metabolism. Feeding dry plasma increases the average daily growth (500 grams per day), positively affects the improvement of the overall metabolism (especially protein metabolism), which leads to an increase in the body's resistance and, as a result, a decrease in the incidence and mortality of animals. Key words: antibiotics, antibiotic resistance, piglets, prebiotics, probiotics, synbiotics, blood products, dry blood plasma.

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Shevchenko, A. A., and A. V. Toropyno. "THERAPY FOR ESCHERICHIOSIS OF NEWBORN CALVES." Polythematic Online Scientific Journal of Kuban State Agrarian University, May 29, 2020. http://dx.doi.org/10.21515/1990-4665-159-005.

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Kuznetsova, Svetlana A., Galina P. Skvortsova, Anastasya A. Moroz, Svetlana Schislenko, Irina V. Korolkova, Nikolai V. Chesnokov, and Boris N. Kuznetsov. "Enterosorbents from Abies Bark and their Therapeutic and Preventive Properties in Experimental Escherichiosis of Animals." Journal of Siberian Federal University. Chemistry, March 2020, 122–32. http://dx.doi.org/10.17516/1998-2836-0158.

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Methods have been developed for the preparation of enterosorbents by treating crushed abies bark with the 1.5% aqueous of NaOH solution or by extraction with hexane. It was found that all obtained enterosorbents have good sorption properties with respect to gelatin and methylene blue. The highest sorption activity for methylene blue was shown by a sample, obtained by extraction of abies bark with hexane (97.0 mg/g), and for gelatin sorption activity – a sample, obtained by treatment of abies bark with alkali (225.0 mg/g). It was found that the enterosorbent, obtained by treatment of abies bark with alkali solution both at a dose of 0.15 g per kg of animal weight (g/kg) and at a dose of 0.20 g/kg and the enterosorbent, obtained by extraction of abies bark with hexane at a dose of 0.20 g/kg, have the highest efficiency for the treatment and prevention of acute experimental Escherichiosis. From the considered methods of obtaining enterosorbents from abies bark for the treatment and prevention of gastrointestinal infections of animals, it is recomended to use a method based on treatment of abies bark with alkalai solution and apply the drug in a dose of 0.15 g/kg

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Mironova, Anna, Suleiman Suleymanov, Dmitry Ivanov, Mikhail Obukhov, and Alexandra Gospodinova. "Pathomorphological Changes in Calves with Intrauterine Colibacteriosis." KnE Life Sciences, April 5, 2021. http://dx.doi.org/10.18502/kls.v0i0.8931.

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Cattle provide valuable food and raw materials for various types of industry, which is why the successful development of breeding of this type of farm animal plays an important role for the economy of the Russian Federation. Unfortunately, calves, due to stressful conditions and improper maintenance, feeding and exploitation of pregnant cows, are susceptible to diseases of the gastrointestinal tract. Colibacteriosis is one of the main causes of loss of productivity and mortality of young cattle. Therefore, the study of methods for the prevention, diagnosis and treatment of this disease is an urgent problem of veterinary medicine. This article examines the clinical and pathological methods of studying calves with intrauterine colibacteriosis. The results are presented in the form of photographs of pathomorphological changes in organs and tissues with a list of eleven pathological diagnoses inherent in escherichiosis, such as dehydration, exhaustion and anemia; hemorrhagic diathesis; acute serous lymphadenitis; acute serous splenitis (septic spleen); fatty degeneration and hemorrhages in the liver; acute catarrhal bronchopneumonia, mainly of the apical and cardiac lobes; hyperemia and hemorrhage under the epi- and endocardium; acute alterative myocarditis; acute catarrhal or hemorrhagic gastroenterocolitis; hemorrhagic or acute catarrhal with banded hemorrhage proctitis; and acute serous nephritis. For the study, we selected 16 calves of Holstein-Friesian and Brown Schwyz breed from the age of newborns to two weeks of age with symptoms of toxic dyspepsia. For the pathological studies, autopsiesof 10 corpses of calves from 1 to 5 days of life were performed. Keywords: intrauterine colibacteriosis, calves, pathological changes

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Popov, V. S., G. A. Svazlyan, and N. M. Naumov. "Immunomodulatory therapy for bacterial infections in pregnant sows." Veterinaria i kormlenie, no. 1 (February 2022). http://dx.doi.org/10.30917/att-vk-1814-9588-2022-1-10.

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The paper presents data reflecting the titers of agglutinins to specific antigens in the blood of pregnant sows and the dynamics of T and B lymphocytes, with specific immunocorrection of general intestinal diseases with the OKZ vaccine containing the antigens of colibacillosis, salmonellosis, Klebsiellosis and Proteus infection, and PPS containing Sal.cholerae suis , Sal.typhimurium, Pasteurella multocida (serovars A, B, D) and streptococci (serogroups C and R) in combination with the immunomodulator metallosuccinate. It is known that the most common pathogens are opportunistic bacteria of the Enterobacteriaceae family: Escherichia coli, Proteus vulgaris, Klebsiella pneumonia, Morganella morgani, which are widely represented in the environment, and are also resident representatives of the normal microflora of animals. In this regard, the specific prevention of bacterial infections with the simultaneous stimulation of nonspecific immunity in pregnant sows is of scientific and practical importance. Specific prophylaxis of sows, in combination with metallosuccinate, increases agglutinin titers, respectively, to pasteurellosis antigen within 19.8% - 24.7%, salmonella antigens (S. cholerae suis 2.2 times, S. typhimurium 2.9 times) and by 77.9% after the second injection, to the Escherichiosis antigen by 9.2% -67.3% and to the streptococcal antigen by 2.8 times compared with the animals of the control group. An increase in agglutinin titers was found without the use of specific drugs, which characterizes metallosuccinate as a drug with antigenicity. The use of metallosuccinate in sows using specific biological products made it possible to activate the synthesis of B-lymphocytes within 47.7% after the first injection, by 50.7% 10 days after the second injection and by 42.6% 20 days after the second injection. Thus, in the studies carried out, a strategy and basic principles of simultaneous immune and metabolic correction of the body of pregnant sows have been proposed.

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Smirnova, L. I., S. A. Makavchik, A. A. Sukhinin, S. V. Pankratov, and T. N. Rozhdestvenskaya. "Sensitivity to antibacterial preparations Campylobacter jejuni isolated from poultry products." Veterinaria i kormlenie, no. 6 (December 2021). http://dx.doi.org/10.30917/att-vk-1814-9588-2021-6-14.

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Along with salmonellosis and Escherichiosis, intestinal campylobacteriosis caused by thermophilic campylobacter, primarily Campylobacter jejuni, is widespread. When studying the biological properties of isolated pathogens, great importance is attached to determining their sensitivity to antibacterial preparations and identifying preparation resistance. Livestock products, including poultry, are one of the most common sources of pathogenic campylobacter. Since antibiotics are often used in the process of growing and obtaining poultry meat, the acquired resistance of the campylobacter bacteria that colonize poultry products to the drugs used in veterinary medicine is likely. The spread of antibiotic-resistant strains in future can lead to decrease of the therapeutic effect in the treatment of severe forms of campylobacteriosis. In this article we present the results of determining the antibiotic resistance of C. jejuni cultures isolated from group samples of poultry products purchased in St. Petersburg in comparison with the antibiotic resistance of C. jejuni cultures isolated from group samples of chickens of the control groups, hatched without use of any antimicrobial preparations. Based on the results of our studies, we found that C. jejuni cultures isolated from group samples of poultry products purchased in St. Petersburg retail network have broad antibiotic resistance and are resistant to the effects of amoxicillin, ampicillin, carbenicillin, erythromycin, azithromycin, cephalothin, gentamycin, kanamycin, tetracycline, doxycycline, lincomycin, ciprofloxacin and co-trimoxazole, but are sensitive to the action of nalidixic acid, imipenem, meropenem, chloramphenicol and furazolidone. Cultures of C. jejuni isolated from group samples of poultry products purchased in St. Petersburg retail network have a broader antibiotic resistance to antimicrobial drugs of various pharmacological groups compared to cultures of C. jejuni isolated from group samples of chickens from the control group. Broader antibiotic resistance of C. jejuni cultures isolated from group samples of poultry products purchased in St. Petersburg retail network is most likely associated with the use of various antimicrobial drugs when growing poultry in industrial poultry farming.

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Yaromchyk, Y. P., P. А. Krasochko, P. P. Krasochko, V. M. Eremets, and T. A. Skotnikova. "Selection the optimal dose of application associated vaccine against rota-, coronavirus infection and colibacillosis in calves." Veterinaria i kormlenie, no. 1 (February 2021). http://dx.doi.org/10.30917/att-vk-1814-9588-2021-1-17.

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The main rules for choosing the means of specific prophylactic against colibacillosis (escherichiosis) in cattle is the need to match the antigenic spectrum of the vaccine strains with epizootic strains isolated by diagnostic veteri-nary institutions from pathological material taken from dead calves. Only in this case one should expect high results of the preventive efficacy of the applied vac-cines. The design of vaccines based on pathogenicity factors of bacterias is the most promising direction in the development of new bioproducts of specific pre-vention of infectious diseases of farm animals. Researchs in the areas to choice the optimal doses and ratios of monocompo-nents, determining the optimal immunizing dose, and choosing a adjuvant is an im-portant part of research work on the creation of vaccines. We completed work to establish the optimal dose when using the associated vaccine against rota-, coronavirus infection and colibacillosis of cattle by vaccinat-ing cows in different doses of the test vaccine. Subsequently, serological studies of the blood of animals were carried out, according to the results of which the indica-tors of the best immune response were determined by comparing the established levels of biosynthesis of specific antibodies from animals of the experimental and control groups. A number of indicators of possible reactogenicity of the tested vac-cine were also studied. When conducting studies of blood serum of cows, the indi-rect hemagglutination reaction and agglutination reaction were performed. Serolog-ical blood tests carried out were accompanied by the necessary controls to ensure the reliability of the results. According to the results of studies of blood serum of cows in the experimental groups, immunization of cows with an associated vaccine against rota-, coronavirus infection and colibacillosis of cattle in different volumes led to a significant level of antiviral and antibacterial antibodies. The optimum dose for virus antigens with infectious titres from 7,0 lg and 5,5-TCI50/sm3 for vaccinated cows forms 1,5 sm3 for each viral monocomponents. The optimum dose for each vaccine strains E.coli F4, F5, Аtt25, F41 and 987P forms from 1,5-2,5 milliard bacterial cells for each bacterial monocomponents.

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Emirova, Khadizha, Evgeniya Tolstova, Olga Orlova, Alexandr Muzurov, Tatiana Pankratenko, Tatiana Abaseeva, Galina Generalova, and Sofya Mstislavskaya. "P1810INFECTION TRIGGERS AS A COMPLEMENT-ACTIVATING STATE IN ATYPICAL HEMOLYTIC-UREMIC SYNDROME IN CHILDREN." Nephrology Dialysis Transplantation 35, Supplement_3 (June 1, 2020). http://dx.doi.org/10.1093/ndt/gfaa142.p1810.

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Abstract Background and Aims Atypical hemolytic-uremic syndrome (aHUS) is a rare life-threatening disease, which is based on the dysregulation of the complement system. Different complement-activating factors is known for aHUS, including infections, vaccination, concomitant diseases, pregnancy, surgical interventions, transplantation, etc. In 24% of cases, a connection with diarrheal prodrome is revealed, in 18% - with acute respiratory infections. The diagnosis of aHUS is established if other forms of thrombotic microangiopathy (TMA) are rejected: HUS associated with Stx-producing strains of E. Coli and Shigella dysenteriae (STEC / Stx - HUS), TTP. We analyze the etiology of trigger events in children with aHUS. Method An analysis of 165 case histories of children with aHUS was carried out for the period from 2000 to 2019. Of these, 70 are boys and 95 are girls. The average age was 4.83 ± 3.84 years (from 2.5 months to 17.8 years). The diagnosis of aHUS was established on the basis of microangiopathic hemolysis, thrombocytopenia, organ dysfunction and recurrent TMA activity. 66 patients were genetically screened, among which mutations in genes encoding complement proteins were detected in 23 (34.9%) children. Results The prodromal period of patients with aHUS was characterized by fever (53.9%), weakness (42.4%), decreased appetite (27.3%), catarrhal symptoms (20.6%), nausea (17.6%), and vomiting ( 63%), abdominal syndrome (26.7%), diarrhea (38.8%), hemocolitis (12.1%). Among the trigger events, acute intestinal infections accounted for 44.2% of cases, acute viral infections - 26.0% (with catarrhal syndrome - 20.6%, intestinal - 5.4%), herpetic infection (CMV, VEB, HSV 1 type) was found in 3% of cases. In 10.3% of children, the TMA symptom complex developed after vaccination with mainly live attenuated vaccines, in 4.2% against other rare conditions (trauma, taking an unknown drug, tonsillitis, etc.). In 12.1% of cases, the disease manifested itself without a complement-activating state. E.coli was confirmed in 16 observations (EHEC - 12, EagEC - 2, EPEC - 2), shigellosis (Shigella Flexner 6) was detected in 1 case. The nature of viral diarrhea was identified in 8 patients: rotovirus RNA - 4, adenovirus DNA - 2, norovirus RNA - 2, type 5 Coxsackie RNA - 1. In the case of TMA after E.coli and shigella, the initial diagnosis was STEC / STx - HUS, which was subsequently transformed into aHUS due to the recurrent TMA activity with persistent dialysis-dependent AKI, and the addition of extrarenal symptoms not associated with volume overload. Conclusion Infectious diseases are the most common complement-activating condition for the implementation of aHUS in children. Among them, in most cases, diarrheal prodrome is associated with acute intestinal infections, which differs from population data (44.2% vs 24%). With the development of TMA in children with escherichiosis/shigellosis, repeated episodes of hemolysis, platelet consumption, the appearance of extrarenal symptoms with normalization of hematological parameters, the detection of pathogenic mutations in complement genes and the positive effect of complement blocking therapy (eculizumab) confirms the diagnosis of aHUS in this category of patients. To exclude or confirm aHUS in these cases, monitoring of clinical and laboratory data is necessary.

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Journal articles on the topic 'Escherichiosis'

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