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Journal articles on the topic 'Esterases. eng'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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1

Ferrer-Parra, Lorena, Dolores Itzel López-Nicolás, Rocío Martínez-Castillo, et al. "Partial characterization of esterases from Fusarium culmorum grown in media supplemented with di (2-ethyl hexyl phthalate) in solid-state and submerged fermentation." Mexican Journal of Biotechnology 3, no. 1 (2018): 82–94. http://dx.doi.org/10.29267/mxjb.2018.3.1.83.

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Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in the polyvinyl chloride (PVC) industry. The indiscriminate use of various products manufactured with PVC, causes this plasticizer to be considered a contaminant. Fusarium culmorum is a phytopathogenic fungus that has the ability to produce esterase enzymes. Esterases are of great importance because they can break the ester bonds present in the plasticizers. In this work, the activity of esterases produced by F. culmorum grown in media supplemented with different concentrations of DEHP (1500 and 2000 mg/L) in solid-state fermentation an

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2

Mentlein, R., and E. Heymann. "Hydrolysis of retinyl esters by non-specific carboxylesterases from rat liver endoplasmic reticulum." Biochemical Journal 245, no. 3 (1987): 863–67. http://dx.doi.org/10.1042/bj2450863.

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The four most important non-specific carboxylesterases from rat liver were assayed for their ability to hydrolyse retinyl esters. Only the esterases with pI 6.2 and 6.4 (= esterase ES-4) are able to hydrolyse retinyl palmitate. Their specific activities strongly depend on the emulsifier used (maximum rate: 440 nmol of retinol liberated/h per mg of esterase). Beside retinyl palmitate, these esterases cleave palmitoyl-CoA and monoacylglycerols with much higher rates, as well as certain drugs (e.g. aspirin and propanidid). However, no transacylation between palmitoyl-CoA and retinol occurs. Retin

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3

Ríos-González, Naomi Surellem, Ángel González-Márquez, and Carmen Sánchez. "Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation." Mexican journal of biotechnology 4, no. 1 (2019): 51–60. http://dx.doi.org/10.29267/mxjb.2019.4.1.51.

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Di (2-ethyl hexyl) phthalate (DEHP) is a plasticizer present in various products, mainly those made with PVC. This phthalate has attracted attention due to its important participation in the contamination of the environment. It has been reported as an endocrine-disrupting compound in mammals. Fusarium culmorum is a phytopathogenic fungus able to degrade DEHP, because it produces esterases, which are enzymes capable to break down ester bonds present in the structure of phthalates. In this research, growth, protein content and esterases activity by biochemical tests and polyacrylamide gel electr

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4

Bouzid, Ourdia, Eric Record, Michèle Asther, et al. "Exploration of members ofAspergillussectionsNigri,Flavi, andTerreifor feruloyl esterase production." Canadian Journal of Microbiology 52, no. 9 (2006): 886–92. http://dx.doi.org/10.1139/w06-046.

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The ability of members of Aspergillus sections Nigri, Flavi, and Terrei to produce feruloyl esterases was studied according to their substrate specificity against synthetic methyl esters of hydroxycinnamic acids. Type A feruloyl esterases (FAEA), induced during growth on cereal-derived products, show a preference for the phenolic moiety of substrates that contain methoxy substitutions, as found in methyl sinapinate, whereas type B feruloyl esterases (FAEB) show a preference for the phenolic moiety of substrates that contain hydroxyl substitutions, as occurs in methyl caffeate. All the strains

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5

Julinawati, Syarifah, Oke Rina, Rosmayanti Rosmayanti, Rafita Ramayati, and Rusdidjas Rusdidjas. "Urine dipstick test for diagnosing urinary tract infection." Paediatrica Indonesiana 53, no. 6 (2013): 315. http://dx.doi.org/10.14238/pi53.6.2013.315-9.

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Background Urinary tract infection (UTI) is a common diseasein children. Approximately 3-5% of girls and 1 % of boys developa UTI. In children, prompt treatment is essential because UTImay be a risk factor for developing renal insufficiency or end stagerenal disease. Howevet; prompt treatment depends on having arapid diagnosis. Urine dipstick testis a useful and commonly usedbecause it is low cost and gives rapid results, compared to urinecultures for diagnosing UTis. However, the diagnostic accuracyof the urine dipstick test is debatable.Objective To compare urine dipstick test (leukocyte est

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6

Afify, Adel S., Yoshimitsu Yamazaki, Yu-ichi Kageyama, et al. "Different Expression of Esterase Variants in Rat Hepatic and Hepatom a-Derived Cell Lines Detected by Electrophoresis." Zeitschrift für Naturforschung C 50, no. 9-10 (1995): 664–68. http://dx.doi.org/10.1515/znc-1995-9-1011.

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Abstract Esterases in nine rat hepatic and hepatoma-derived cell lines and normal rat liver homogenate were detected by polyacrylamide gel electrophoresis coupled with active staining with a-naphthyl acetate or butyrate as a substrate. The esterase band patterns of the non-cancerous and oncogene-transformed cell lines were alike, but different from those of hepatoma cell lines and normal rat liver homogenate. The former groups of cells might have completely lost the characteristics of rat liver parenchymal cells, or else they might have their origin at cells other than liver parenchyma. The es

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7

Robbi, M., H. Beaufay, and J. N. Octave. "Nucleotide sequence of cDNA coding for rat liver pI 6.1 esterase (ES-10), a carboxylesterase located in the lumen of the endoplasmic reticulum." Biochemical Journal 269, no. 2 (1990): 451–58. http://dx.doi.org/10.1042/bj2690451.

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A commercial rat liver cDNA library in lambda gt11 was screened with a rabbit antiserum to native pI 6.1 esterase (ES-10). The inserts of the immunoreactive clones were short (0.9-1.1 kbp). One of these was used as a probe to rescreen the library, yielding 30 clones, two of which contained relatively long (approx. 1.9 kbp) and widely overlapping cDNA inserts. They did not contain the first two nucleotide residues of the initiator codon, nor the 5′-end untranslated portion of the mRNA. These were derived from a home-made rat liver cDNA library in lambda gt11, screened with an oligonucleotide co

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8

Hemingway, J., N. Hawkes, L. Prapanthadara, K. G. I. Jayawardenal, and H. Ranson. "The role of gene splicing, gene amplification and regulation in mosquito insecticide resistance." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 353, no. 1376 (1998): 1695–99. http://dx.doi.org/10.1098/rstb.1998.0320.

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The primary routes of insecticide resistance in all insects are alterations in the insecticide target sites or changes in the rate at which the insecticide is detoxified. Three enzyme systems, glutathione S–transferases, esterases and monooxygenases, are involved in the detoxification of the four major insecticide classes. These enzymes act by rapidly metabolizing the insecticide to non–toxic products, or by rapidly binding and very slowly turning over the insecticide (sequestration). In Culex mosquitoes, the most common organophosphate insecticide resistance mechanism is caused by co–amplific

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9

de Vries, Ronald P., and Jaap Visser. "Regulation of the Feruloyl Esterase (faeA) Gene from Aspergillus niger." Applied and Environmental Microbiology 65, no. 12 (1999): 5500–5503. http://dx.doi.org/10.1128/aem.65.12.5500-5503.1999.

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ABSTRACT Feruloyl esterases can remove aromatic residues (e.g., ferulic acid) from plant cell wall polysaccharides (xylan, pectin) and are essential for complete degradation of these polysaccharides. Expression of the feruloyl esterase-encoding gene (faeA) fromAspergillus niger depends on d-xylose (expression is mediated by XlnR, the xylanolytic transcriptional activator) and on a second system that responds to aromatic compounds with a defined ring structure, such as ferulic acid and vanillic acid. Several compounds were tested, and all of the inducing compounds contained a benzene ring which

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10

Wei, L., S. Gao, W. Xiong, et al. "Latrophilin mediates insecticides susceptibility and fecundity through two carboxylesterases, esterase4 and esterase6, in Tribolium castaneum." Bulletin of Entomological Research 109, no. 4 (2019): 534–43. http://dx.doi.org/10.1017/s0007485318000895.

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AbstractLatrophilin (LPH) is known as an adhesion G-protein-coupled receptor which involved in multiple physiological processes in organisms. Previous studies showed that lph not only involved the susceptibility to anticholinesterase insecticides but also affected fecundity in Tribolium castaneum. However, its regulatory mechanisms in these biological processes are still not clear. Here, we identified two potential downstream carboxylesterase (cce) genes of Tclph, esterase4 and esterase6, and further characterized their interactions with Tclph. After treatment of T. castaneum larvae with carbo

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11

Hortsch, M., N. H. Patel, A. J. Bieber, Z. R. Traquina, and C. S. Goodman. "Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis." Development 110, no. 4 (1990): 1327–40. http://dx.doi.org/10.1242/dev.110.4.1327.

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Drosophila neurotactin is a transmembrane glycoprotein with an apparent molecular mass of 135 × 10(3). Neurotactin is regionally expressed at the cellular blastoderm stage; later in embryogenesis the expression of the protein becomes restricted to cells of the peripheral and central nervous system. Immunocytochemical localization shows neurotactin protein at points of cell-cell contact. Using the anti-neurotactin monoclonal antibody BP-106, a neurotactin cDNA was isolated that encodes a 846 residue polypeptide. The chromosomal location of the neurotactin gene is 73C. The extracellular domain a

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12

Nghi, Do Huu, Britta Bittner, Harald Kellner та ін. "The Wood Rot Ascomycete Xylaria polymorpha Produces a Novel GH78 Glycoside Hydrolase That Exhibits α-l-Rhamnosidase and Feruloyl Esterase Activities and Releases Hydroxycinnamic Acids from Lignocelluloses". Applied and Environmental Microbiology 78, № 14 (2012): 4893–901. http://dx.doi.org/10.1128/aem.07588-11.

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ABSTRACTSoft rot (type II) fungi belonging to the family Xylariaceae are known to substantially degrade hardwood by means of their poorly understood lignocellulolytic system, which comprises various hydrolases, including feruloyl esterases and laccase. In the present study, several members of the Xylariaceae were found to exhibit high feruloyl esterase activity during growth on lignocellulosic materials such as wheat straw (up to 1,675 mU g−1) or beech wood (up to 80 mU g−1). Following the ester-cleaving activity toward methyl ferulate, a hydrolase ofXylaria polymorphawas produced in solid-sta

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13

Scherer and Bircher. "Wenn's eng wird - oder das Angioödem des Oropharynx." Therapeutische Umschau 61, no. 5 (2004): 341–47. http://dx.doi.org/10.1024/0040-5930.61.5.341.

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Angioödeme können zahlreiche Ursachen haben, vor allem zu nennen sind dabei Medikamente, Nahrungsmittel, Insektenstiche, C1-Esterase-Inhibitor-Mangel und andere. Häufig lässt sich allerdings keine singuläre Ursache finden. Es wird ein Überblick über das klinische Bild, die Ätiopathologie sowie therapeutische Optionen gegeben.

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14

Field, L. M., M. S. Williamson, G. D. Moores, and A. L. Devonshire. "Cloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, Myzus persicae (Sulzer)." Biochemical Journal 294, no. 2 (1993): 569–74. http://dx.doi.org/10.1042/bj2940569.

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Full-length cDNA clones encoding the esterases (E4 and FE4) that confer insecticide resistance in the peach-potato aphid [Myzus persicae (Sulzer)] were isolated and characterized. The E4 cDNA contained an open reading frame of 1656 nucleotides, coding for a protein of 552 amino acids. The FE4 cDNA shared 99% identity with E4 over this region, the most important difference being a single nucleotide substitution resulting in the FE4 mRNA having an extra 36 nucleotides at the 3′ end. The derived amino acid sequences for the N-terminus of E4 and FE4 were identical, with the first 23 residues being

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15

Medina-Flores, Henry, Angel González-Márquez, and Carmen Sánchez. "Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation." Mexican journal of biotechnology 5, no. 4 (2020): 64–79. http://dx.doi.org/10.29267/mxjb.2020.5.4.64.

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Tween 80 is a widely used non-ionic surfactant that is added to culture media to make hydrophobic substrate available to microorganisms. Because of this surfactant widespread use, it is important to understand how it affects microbial growth and enzyme production. In this work, the effect of different concentrations (100, 400 and 600 l/L; v/v) of Tween 80 (as the sole carbon source) on the biomass production, esterase activities (assessed through biochemical tests and zymographic assays) and protein content of Fusarium culmorum grown in liquid fermentation was determined. The specific growth

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16

Bracco, Paula, Nelleke van Midden, Epifanía Arango, et al. "Bacillus subtilis Lipase A—Lipase or Esterase?" Catalysts 10, no. 3 (2020): 308. http://dx.doi.org/10.3390/catal10030308.

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The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a minimal α/β hydrolase fold enzyme, the Bacillus subtilis lipase A (BSLA), and compared to Candida antarctica lipase B (CALB), a proven lipase. Both hydrolases show activity in dry solvents and this proves BSLA to be a lipase. Overall, this demonstrates the value of this additional parameter to dist

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17

Shenhar-Tsarfaty, S., N. Waiskopf, K. Ofek, et al. "Atherosclerosis and arteriosclerosis parameters in stroke patients associate with paraoxonase polymorphism and esterase activities." European Journal of Neurology 20, no. 6 (2013): 891–98. http://dx.doi.org/10.1111/ene.12074.

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18

Saha, Dhiraj. "Host plant-based variation in fitness traits and major detoxifying enzymes activity in Scirtothrips dorsalis (Thysanoptera: Thripidae), an emerging sucking pest of tea." International Journal of Tropical Insect Science 36, no. 03 (2016): 106–18. http://dx.doi.org/10.1017/s1742758416000102.

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AbstractScirtothrips dorsalisHood is a polyphagous species and an important sucking pest of tea (Camellia sinensis) (Theaceae). The fitness traits ofS. dorsalison two alternative host plants:Capsicum annuumL. (chilli) (Solanaceae) andRicinus communis(castor oil plant) (Euphorbiaceae) and onC. sinensisand corresponding levels of defence enzymes was studied. The study revealed thatC. sinensisis the more suitable host ofS. dorsalisbased on faster development (13.6 days) compared to the alternative hosts,C. annuum(15.5 days) andR. communis(16.7 days), a higher fecundity (C. sinensis: 11.4 eggs;C.

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19

Cohen, Robert E., Peter S. Creticos, and Philip S. Norman. "The Effects of Guided Imagery (GI) on Allergic Subjects' Responses to Ragweed-Pollen Nasal Challenge: An Exploratory Investigation." Imagination, Cognition and Personality 13, no. 3 (1994): 259–69. http://dx.doi.org/10.2190/56uu-uqpc-r2ga-c651.

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This study investigated the effects of guided imagery (GI) on allergic subjects' responses to ragweed-pollen nasal challenge. Complete data was obtained for fourteen subjects ranging from twenty-one to sixty-two years of age. Nasal challenges were performed before and after GI. Dependent variables consisted of two biochemical mediators obtained from nasal secretions and two subject-reported measures of symptoms, respectively: TAME (tosylarginine methyl ester)-esterase; histamine; severity of symptoms (e.g., nasal congestion); and numbers of sneezes. An experimental group ( n = 7) participated

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20

Berger, Rico, Matthias Hoffmann, and Ullrich Keller. "Molecular Analysis of a Gene Encoding a Cell-Bound Esterase from Streptomyces chrysomallus." Journal of Bacteriology 180, no. 23 (1998): 6396–99. http://dx.doi.org/10.1128/jb.180.23.6396-6399.1998.

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ABSTRACT A gene (estA) encoding a 42-kDa cell-bound esterase, EstA, was found to be located 75 bp upstream of the cyclophilin A gene (cypA) of Streptomyces chrysomallus. Western blot analysis revealed the presence of EstA (42 kDa) in cell extracts of S. chrysomallus X2 and Streptomyces lividans. EstA specifically hydrolyzes short-chainp-nitrophenyl esters. EstA formation starts at the end of growth phase, and its activity level remains constant throughout stationary phase. Expression of estA from the melanin (mel) promoter in plasmid pIJ702 led to a substantial increase of total esterase activ

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Mesrobian, R. J. O., M. J. Lyon, and R. R. Gacek. "Esterase Activity in Cat Vestibular End Organs: An Enzymatic Barrier System?" Archives of Otolaryngology - Head and Neck Surgery 113, no. 5 (1987): 543–46. http://dx.doi.org/10.1001/archotol.1987.01860050089022.

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22

Polz, Leo, Helmut Schübel та Joachim Stoekigt. "Characterization of 2β(R)-17-O-AcetylajmaIan: Acetylesterase — a Specific Enzyme Involved in the Biosynthesis of the Rauwolfia Alkaloid Ajmaline". Zeitschrift für Naturforschung C 42, № 4 (1987): 333–42. http://dx.doi.org/10.1515/znc-1987-0403.

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A novel enzyme was isolated, partially purified (217-fold) and characterized from cell suspension cultures of Rauwolfia serpentina Benth. The enzyme catalyzes one of the late biochemical reactions in the biosynthesis of ajmaline by hydrolysis of 17-O-acetylated alkaloids of the ajmalan group forming the appropriate deacetylated compounds. This esterase exhibits an unusually high substrate selectivity and exclusively accepts acetylated ajmaline derivatives with the naturally occurring 2β(R)-configuration. The properties of the enzyme were determined showing an optimum pH at 7.5, an isoelectric

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23

Isman, Murray B., Ruying Feng, and Dan L. Johnson. "DETOXICATIVE ENZYME ACTIVITIES IN FIVE SPECIES OF FIELD-COLLECTED MELANOPLINE GRASSHOPPERS (ORTHOPTERA: ACRIDIDAE)." Canadian Entomologist 128, no. 2 (1996): 353–54. http://dx.doi.org/10.4039/ent128353-2.

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Detoxicative enzyme systems, such as the cytochrome P450 monooxygenases, gluthione S-transferases, and general esterases, have been widely studied in holometabolous insects (e.g. Lepidoptera, Diptera, and Coleoptera). These, and other enzyme systems, play important roles in insecticide resistance, but are also important in insect–host plant relationships, because host range can partially depend on the ability of an insect to cope with putatively toxic allelochemicals in an otherwise suitable host plant (e.g. Lindroth 1989). In some cases, differences in the relative activities of these enzymes

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24

Mamaghani, Manouchehr, and Alireza Alizadehnia. "Enzyme Catalysed Kinetic Resolution of Racemic Methyl 3-acethylbicyclo [2.2.1] hept-5-ene-2-carboxylate using pig's liver esterase†." Journal of Chemical Research 2002, no. 8 (2002): 386–87. http://dx.doi.org/10.3184/030823402103172400.

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Racemic methyl 3- endo-acetylbicyclo[2.2.1]hept-5-ene-2- exo-carboxylate and methyl 3- exo-acetylbicyclo[2.2.1]hept-5-ene-2- endo-carboxylate were prepared (as a 1:1 mixture in 98% yield) from (E)-methyl 4- oxo-2-pentenoate, using Diels–Alder methodology. The mixture was separated chromatographically and resolved into the enantiomers by pig liver esterase (PLE) with low to high ee's (98%).

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25

Bernhard, Joan M., and Samuel S. Bowser. "Novel epifluorescence microscopy method to determine life position of foraminifera in sediments." Journal of Micropalaeontology 15, no. 1 (1996): 68. http://dx.doi.org/10.1144/jm.15.1.68.

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Abstract. Microscopic examination of resin-embedded sedimenl cores has provided information regarding the sedimentary fabric in certain environments (e.g. Watling, 1988) as well as the in situ associations of rose Bengal-stained infaunal foraminifera (e.g. Frankel, 1970, 1974). Unfortunately, difficulties connected with making and examining these resin-embedded cores have limited their widespread use in studying meioinfauna. In particular, sediment grains may obscure specimens when viewed by transmitted light microscopy. Also, using rose Bengal to identify live foraminifera can be problematic

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Vucetic, Andja, Olivera Petrovic-Obradovic, J. Margaritopoulos, and P. Skouras. "Establishing the resistance of Myzus persicae (Sulzer) by molecular methods." Archives of Biological Sciences 60, no. 3 (2008): 493–99. http://dx.doi.org/10.2298/abs0803493v.

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In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be m

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Hanker, J., G. Strauss, P. Yates, and B. Giammara. "The light and electron microscopic demonstration of B-lymphocytes with a new Mg++/ATPase method." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 770–71. http://dx.doi.org/10.1017/s042482010012816x.

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Helper T-lymphocytes are readily demonstrated for light microscopy by an enzyme cytochemical method for nonspecific esterase with the substrate α-naphthyl butyrate acetate. It was recently shown that a modification of this esterase method also demonstrates immature and cultured helper T cells. Although Harigaya et al. were able to demonstrate B-cells in B lymphomas and in some smears of whole blood, they were unable to demonstrate B lymphocytes in enriched leukocyte preparations. Studies in our laboratories have shown that a new method for the demonstration of myofibrillar ATPase could be also

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Lynch, J. P., D. Prema, J. D. Van Hamme, J. S. Church, and K. A. Beauchemin. "Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant." Canadian Journal of Animal Science 94, no. 4 (2014): 697–704. http://dx.doi.org/10.4141/cjas-2014-086.

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Lynch, J. P., Prema, D., Van Hamme, J. D., Church, J. S. and Beauchemin, K. A. 2014. Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant. Can. J. Anim. Sci. 94: 697–704. This study investigated the effects of two fibrolytic enzyme products, applied at baling alone or in combination with a ferulic acid esterase-producing bacterial additive, on the ensilage dynamics, chemical composition and digestibility of alfalfa haylage. Five replicate wrapped bales were produce

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Pavičić-Hamer, Dijana, Ana Baričević, Marco Gerdol, and Bojan Hamer. "Mytilus galloprovincialis Carbonic Anhydrase II: Activity and cDNA Sequence Analysis." Key Engineering Materials 672 (January 2016): 137–50. http://dx.doi.org/10.4028/www.scientific.net/kem.672.137.

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The esterase activity of carbonic anhydrase (CA) was investigated in mussels sampled at 24 locations along the Croatian coast of Adriatic Sea. The gills were the target tissue because the respiratory, ionic transport and pH regulatory enzyme function of CA and its potential usage as biomarkers of environmental pollution was the main topic. Total esterase activity was measured in cytosolic fraction by colorimetric end-point reaction using p-nitrophenyl acetate as enzyme substrate. CA activity was estimated by the same enzymatic reaction using acetazolamide as a specific CA inhibitor. The result

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Madani, W., S. Kermasha, and A. Versari. "Characterization of Tyrosinase- and Polyphenol Esterase-Catalyzed End Products Using Selected Phenolic Substrates." Journal of Agricultural and Food Chemistry 47, no. 6 (1999): 2486–90. http://dx.doi.org/10.1021/jf981125e.

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BASAVAIAH, D., and P. D. RAO. "ChemInform Abstract: Crude Chicken Liver Esterase Mediated Resolution of Homoallyl Alcohols, e.g. (II)." ChemInform 25, no. 47 (2010): no. http://dx.doi.org/10.1002/chin.199447104.

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Ojeda, Nicolás, Constanza Cárdenas, and Sergio Marshall. "Interaction of the Amino-Terminal Domain of the ISAV Fusion Protein with a Cognate Cell Receptor." Pathogens 9, no. 6 (2020): 416. http://dx.doi.org/10.3390/pathogens9060416.

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The infectious salmon anemia virus (ISAV), etiological agent of the disease by the same name, causes major losses to the salmon industry. Classified as a member of the Orthomyxoviridae family, ISAV is characterized by the presence of two surface glycoproteins termed hemagglutinin esterase (HE) and fusion protein (F), both of them directly involved in the initial interaction of the virus with the target cell. HE mediates receptor binding and destruction, while F promotes the fusion process of the viral and cell membranes. The carboxy-terminal end of F (F2) possesses canonical structural charact

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Oceguera-Cervantes, Alejandro, Agustín Carrillo-García, Néstor López, et al. "Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N-Methylpyrrolidone." Applied and Environmental Microbiology 73, no. 19 (2007): 6214–23. http://dx.doi.org/10.1128/aem.01230-07.

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ABSTRACT Two bacterial strains (BQ1 and BQ8) were isolated from decomposed soft foam. These were selected for their capacity to grow in a minimal medium (MM) supplemented with a commercial surface-coating polyurethane (PU) (Hydroform) as the carbon source (MM-PUh). Both bacterial strains were identified as Alicycliphilus sp. by comparative 16S rRNA gene sequence analysis. Growth in MM-PUh showed hyperbolic behavior, with BQ1 producing higher maximum growth (17.8 ± 0.6 mg·ml−1) than BQ8 (14.0 ± 0.6 mg·ml−1) after 100 h of culture. Nuclear magnetic resonance, Fourier transform infrared (IR) spec

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Nouraei, Hasti, Mehdi Ghaderian Jahromi, Leila Razeghian Jahromi, Kamiar Zomorodian, and Keyvan Pakshir. "Potential Pathogenicity of Candida Species Isolated from Oral Cavity of Patients with Diabetes Mellitus." BioMed Research International 2021 (May 26, 2021): 1–6. http://dx.doi.org/10.1155/2021/9982744.

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Introduction. In the recent decade, the increased immunocompromised population such as diabetic patients makes a high incidence of invasive Candida infections. Diabetes mellitus is the most common endocrine metabolic disorder, and diabetic patients are more susceptible to oral candidiasis infection. Candidiasis is an opportunistic fungal infection caused by many species of Candida. Secretion of exoenzymes plays an important role in the virulence and pathogenesis of Candida species. The aim of this study was to evaluate the potential role of phospholipase, esterase, and hemolytic activity of Ca

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Ferrer-Parra, Lorena, Dolores Itzel López-Nicolás, Rocío Martínez-Castillo, et al. "Partial characterization of esterases from Fusarium culmorum grown in media supplemented with di (2-ethyl hexyl phthalate) in solid-state and submerged fermentation." Mexican Journal of Biotechnology 3, no. 1 (2018): 82–94. http://dx.doi.org/10.29267/mxjb.2018.3.1.82.

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Kota, Swathi, C. Vijaya Kumar, and Hari S. Misra. "Characterization of an ATP-regulated DNA-processing enzyme and thermotolerant phosphoesterase in the radioresistant bacterium Deinococcus radiodurans." Biochemical Journal 431, no. 1 (2010): 149–57. http://dx.doi.org/10.1042/bj20100446.

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A multiprotein DNA-processing complex identified from Deinococcus radiodurans exhibits uncharacterized ATP-sensitive nuclease functions. DR0505 was one of the 24 polypeptides identified from the complex. It contains two 5′ nucleotidase motifs, one is at the C-terminal end of the N-terminal CPDD (calcineurin phosphodiesterase domain), with the second at the C-terminal end of the protein. Recombinant DR0505 showed both phosphomonoesterase and phosphodiesterase activities with chromogenic substrates, showing higher affinity for bis-(p-nitrophenyl) phosphate than for p-nitrophenyl phosphate. The e

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Ramaswamy, Sonny B., Shengqiang Shu, George N. Mbata, et al. "Role of juvenile hormone-esterase in mating-stimulated egg development in the moth Heliothis virescens." Insect Biochemistry and Molecular Biology 30, no. 8-9 (2000): 785–91. http://dx.doi.org/10.1016/s0965-1748(00)00050-3.

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González-Márquez, Angel, Octavio Loera-Corral, Gustavo Viniegra-González, and Carmen Sánchez. "Production of cutinolytic esterase by Fusarium culmorum grown at different apple cutin concentrations in submerged fermentation." Mexican journal of biotechnology 4, no. 4 (2019): 50–64. http://dx.doi.org/10.29267/mxjb.2019.4.4.50.

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Cutinolytic esterase (i.e., cutinase) is an enzyme that catalyzes the cleavage of ester bonds in cutin and also in diverse soluble and insoluble esters. It has application in several biotechnological areas, acting as biocatalysts in the food industry, in detergents, in biodegradation of polymers and other toxic substances, being important in biorremediation. In this research, specific growth rate, protein content, cutinolytic activity by biochemical tests and polyacrylamide gel electrophoresis, and growth and enzymatic kinetic parameters were determined for F. culmorum grown at different apple

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SULISTYOWATI, E., SULISTYOWATI SULISTYOWATI, S. RUSTINI, S. SUMARTINI, and ABDURRAKHMAN ABDURRAKHMAN. "VARIASI GENETIK BEBERAPA SPESIES KAPAS (Gossypium sp.) BERDASARKAN KERAGAMAN POLA PITA ISOZIM." Jurnal Penelitian Tanaman Industri 15, no. 4 (2020): 174. http://dx.doi.org/10.21082/jlittri.v15n4.2009.174-183.

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<p>ABSTRAK</p><p>Deskripsi aksesi-aksesi kapas berdasarkan karakter morfologinyatelah disusun berdasarkan descriptor list yang disusun oleh IBPGR, akantetapi marka genetik dari aksesi-aksesi tersebut belum diketahui. Penelitianini bertujuan untuk mempelajari keragaman pola-pita isozim Peroksidase(PER), Esterase (EST), dan Aspartate amino transferase (AAT) pada 19aksesi kapas dan kemiripan ke-19 aksesi kapas berdasarkan ketiga isozimtersebut. Penelitian ini dilaksanakan mulai bulan Februari 2008 di RumahKaca Fakultas Pertanian, Universitas Sebelas Maret Surakarta dan analisisi

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Monteiro, Jessica M. S., Juvenil E. Cares, Valdir R. Correa, et al. "Meloidogyne brasiliensis Charchar & Eisenback, 2002 is a junior synonym of M. ethiopica Whitehead, 1968." Nematology 19, no. 6 (2017): 655–69. http://dx.doi.org/10.1163/15685411-00003078.

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The type population ofMeloidogyne brasiliensisis synonymised underM. ethiopicabased on morphological and morphometric similarities, as well as through biochemical, molecular and phylogenetic studies.Meloidogyne ethiopicawas first described in 1968 in Tanzania using specimens from a single egg mass culture on tomato and re-described in 2004 using an isolate from Brazil.Meloidogyne brasiliensiswas described in 2002 in Brazil based on specimens collected from tomato (type population) and pea. Morphological and morphometric studies of their descriptions showed important similarities in major chara

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Nagaraja, T. N., and T. Desiraju. "Effects on Operant Learning and Brain Acetylcholine Esterase Activity in Rats following Chronic Inorganic Arsenic Intake." Human & Experimental Toxicology 13, no. 5 (1994): 353–56. http://dx.doi.org/10.1177/096032719401300511.

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1 Very young and adult Wistar rats were given As5+, 5 mg arsenic kg-1 body weight day-1 (sodium arsenate). 2 Operant learning was tested in a Skinner box at the end of exposure and, in the case of developing animals, also after a recovery period. 3 Acetylcholine esterase (AChE) activity was estimated in discrete brain regions of these animals. 4 The animals exposed to arsenic took longer to acquire the learned behaviour and to extinguish the operant. AChE activity was inhibited in some regions of the brain.

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Leong, Edmund C. W., and S. H. Ho. "In vitro inhibition of esterase activity in Liposcelis bostrychophila bad. and L. entomophila (end.) (Psocoptera: Liposcelididae)." Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 110, no. 1 (1995): 121–30. http://dx.doi.org/10.1016/0305-0491(94)00135-h.

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Sun, C., and L. Fiksdal. "Monitoring of biofouling on ultrafiltration hollow fiber membranes by rapid enzyme activity assays." Water Supply 12, no. 2 (2012): 148–56. http://dx.doi.org/10.2166/ws.2012.115.

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The biofouling of an ultrafiltration (UF) membrane unit applied for drinking water treatment was investigated under three different operating conditions, i.e. flux 50, 60 and 70 L m−2 h−1. The biofouling was monitored by analyzing the enzyme (esterase and peptidase) activity on the sacrificed membrane fibers of a test unit installed in the same reactor as the membrane pilot module. At a given flux, a decrease of membrane permeability generally corresponded to an increase of enzyme activity. Enzyme activity could therefore be used to predict the permeability decline and potentially be used for

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Despotovic, Dragana, Ljubica Vojcic, Radivoje Prodanovic, Ronny Martinez, Karl-Heinz Maurer, and Ulrich Schwaneberg. "Fluorescent Assay for Directed Evolution of Perhydrolases." Journal of Biomolecular Screening 17, no. 6 (2012): 796–805. http://dx.doi.org/10.1177/1087057112438464.

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Directed evolution offers opportunities to improve promiscuous activities of hydrolases in rounds of diversity generation and high-throughput screening. In this article, we developed and validated a screening platform to improve the perhydrolytic activity of proteases and likely other hydrolases (e.g., lipases or esterases). Key was the development of a highly sensitive fluorescent assay (sensitivity in the µM range) based on 3-carboxy-7-hydroxycoumarin (HCC) formation. HCC is released through an hypobromite-mediated oxidation of 7-(4′-aminophenoxy)-3-carboxycoumarin (APCC), which enables for

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Niigawa, H., S. Tanimukai, M. Takeda, S. Hariguchi, and T. Nishimura. "EFFECTS OF SDZ ENA-713, NOVEL CHOLINE ESTERASE INHIBITOR, ON LEARNING OF RATS WITH BASAL FOREBRAIN LESIONS." Clinical Neuropharmacology 15 (1992): 567B. http://dx.doi.org/10.1097/00002826-199202001-01106.

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Li, Xin, Yue-Meng Wang, Cheng-Feng Sun, Jian-Hao Lv, and Yan-Jun Yang. "Comparative Study on Foaming Properties of Egg White with Yolk Fractions and Their Hydrolysates." Foods 10, no. 9 (2021): 2238. http://dx.doi.org/10.3390/foods10092238.

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As an excellent foaming agent, egg white protein (EWP) is always contaminated by egg yolk in the industrial processing, therefore, decreasing its foaming properties. The aim of this study was to simulate the industrial EWP (egg white protein with 0.5% w/w of egg yolk) and characterize their foaming and structural properties when hydrolyzed by two types of esterase (lipase and phospholipase A2). Results showed that egg yolk plasma might have been the main fraction, which led to the poor foaming properties of the contaminated egg white protein compared with egg yolk granules. After hydrolyzation

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Palczewska, I., and G. Jagodzka. "Cytochemical localization of certain hydrolytic enzymes at various stages of development of Achlya flagellata mycelium." Acta Societatis Botanicorum Poloniae 41, no. 2 (2015): 265–82. http://dx.doi.org/10.5586/asbp.1972.020.

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The standard coupling azo dyes techniques were used to reveal the activities of acid phosphatase, alkaline phosphatase, esterase and β-galactoidase in the vegetative and reproductive cycle of <i>Achlya flagellata</i>. The end-products of the enzymic reactions, with the exception of E 600 sentisive esterese, which is localized in cytoplasm, occured in cytoplasmic granules. These granules are expected to be spherosomes. Acid phosphatase activity is high in differentiating sporangia, in antheridial hyphae and in degenerating oospheres where hydrolytic processes occur. β-galactosidase

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Staege, Martin S., Manuela Hesse, and Daniela Max. "Lipases and Related Molecules in Cancer." Cancer Growth and Metastasis 3 (January 2010): CGM.S2816. http://dx.doi.org/10.4137/cgm.s2816.

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Lipases are enzymes that catalyze the hydrolysis of lipids. Based on protein structures and sequences, lipases can be classified into different protein families. The majority of conventional mammalian lipases are members of the protein super-families of serine esterases and alpha-beta hydrolases. Differential expression of lipases and related alpha-beta hydrolases in tumor cells has been observed. The physiological or patho-physiological functions of these tumor related enzymes are largely unknown. However, lipases are not only involved in energy metabolism but also in the metabolism of bioact

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Süss, Philipp, Sabine Illner, Jan von Langermann, et al. "Scale-Up of a Recombinant Pig Liver Esterase-Catalyzed Desymmetrization of Dimethyl Cyclohex-4-ene-cis-1,2-dicarboxylate." Organic Process Research & Development 18, no. 7 (2014): 897–903. http://dx.doi.org/10.1021/op500129e.

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Coque, Laurent, Purnima Neogi, Catalina Pislariu, et al. "Transcription of ENOD8 in Medicago truncatula Nodules Directs ENOD8 Esterase to Developing and Mature Symbiosomes." Molecular Plant-Microbe Interactions® 21, no. 4 (2008): 404–10. http://dx.doi.org/10.1094/mpmi-21-4-0404.

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In Medicago truncatula nodules, the soil bacterium Sinorhizobium meliloti reduces atmospheric dinitrogen into nitrogenous compounds that the legume uses for its own growth. In nitrogen-fixing nodules, each infected cell contains symbiosomes, which include the rhizobial cell, the symbiosome membrane surrounding it, and the matrix between the bacterium and the symbiosome membrane, termed the symbiosome space. Here, we describe the localization of ENOD8, a nodule-specific esterase. The onset of ENOD8 expression occurs at 4 to 5 days postinoculation, before the genes that support the nitrogen fixa

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