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Molina, Seguin Jessica. "Caracterización clínica, radiológica, pronóstica y del perfil de biomarcadores de los sujetos con ictus isquémico criptogénico no lacunar de mecanismo embólico." Doctoral thesis, Universitat de Lleida, 2019. http://hdl.handle.net/10803/665836.
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Introducció: L’interés per conèixer les característiques dels pacients amb ictus criptogènic no llacunar de mecanisme embòlic (de l’anglès ESUS) ha crescut últimament desde que es disposa de fàrmacs més segurs pel tractament preventiu. Ens planteguem que els ESUS presenten unes característiques clíniques, radiològiques, perfil de biomarcadors (BM) i pronòstic propis que difereixen de les altres etiologies.
Metodologia: Analitzem dues cohorts. La primera cohort prospectiva analitza les característiques clíniques, de neuroimatge, ecocardiogràfiques, BM (NSE, IL-6, hs-CRP, troponina, NT-proBNP, fibrinogen, leucòcits, colesterol tradicional i no-tradicional) i recurrència. La segona cohort determina dades clíniques i de l’evolució intrahospitalaria. Ambdues van analitzar predictors de mal pronòstic en ESUS.
Conclusions: Les característiques clíniques, radiològiques i perfil de BM que presenten els ESUS, els diferencien dels altres subtipus inclòs de la FA crònica i de novo. Això implicaria que no tots els ESUS regeixen el seu origen en una font cardioembòlica no detectada, establint diversos subtipus etiològics.Introducción: El interés por conocer las características de los sujetos con ictus criptogénico no lacunar de mecanismo embólico (del inglés ESUS) ha crecido últimamente tras disponer de fármacos más seguros para el tratamiento preventivo. Nos planteamos que los ESUS presentan características clínicas, radiológicas, perfil de biomarcadores (BM) y pronóstico propios que difieren de las otras etiologías. Metodología: Analizamos dos cohortes. La primera cohorte prospectiva analiza características clínicas, de neuroimagen, ecocardiográficos, BM (NSE, IL-6, hs-CRP, troponina, NT-proBNP, fibrinógeno, leucocitos, colesterol tradicional y no-tradicional) y recurrencia. La segunda cohorte determina datos de la clínica y evolución intrahospitalaria. Ambas analizaron predictores de mal pronóstico en ESUS. Conclusiones: Las características clínicas, radiológicas y perfil de BM que presentan los ESUS, los diferencia de los otros subtipos incluso de la FA crónica y de novo. Esto implicaría que no todos los ESUS rigen su origen en una fuente cardioembólica no detectada, pudiéndose establecer varios subtipos etiológicos.
Introduction: The interest in knowing the characteristics of subjects with embolic source undetermined stroke (ESUS) has increased recently, following the release of safer drugs for preventive treatment. We propose that ESUS subjects exhibit specific clinical and radiological characteristics, biomarker (BK) patterns and a prognosis that differentiates them from other etiologies. Methodology: Two cohorts were analysed. The first prospective cohort analysed clinical, neuroimaging, echocardiography characteristics, BK (NSE, IL-6, hs-CRP, troponin, NT-proBNP, fibrinogen, leukocytes, traditional and non-traditional cholesterol) and recurrence. The second cohort established the clinical and intra-hospital evolution data. In both cohorts, the predictors of poor prognosis in ESUS were analysed. Conclusions: The clinical, radiological and BK profile characteristics of the ESUS differentiates them from the other subtypes, including chronic and the new diagnosis of AF. These results suggest that not all ESUS subjects originate from an undetected cardioembolic source, but in fact could establish several etiological subtypes.
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Bell, K. W. "Baryon production in esup(+)esup(-) annihilation at PETRA." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355729.
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Buchholz, Andreas Hohloch Rüdiger Rathgeber Tim. "Vergleich von Open Source ESBs." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2007. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-33767.
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Hawkes, C. M. "A study of the reaction esup(+)esup(-)-musup(+)musup(-)(gamma) at centre of mass energies up to 46.78GeV." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355754.
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Dias, Delane Pereira de Oliveira. "Uma ferramenta para a visualização de ESTs." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/55/55134/tde-08052007-100008/.
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Expressed Sequence Tags (ESTs) são amostras de trechos de genes, que funcionam como moldes na síntese de proteínas. Como a quantidade de ESTs coletados nos últimos anos é muito grande, o uso de computadores tornou-se imprescindível para a identificação de genes, proteínas e para a descoberta de genes homólogos. Este trabalho propõe uma metodologia e implementa uma ferramenta para a visualização de ESTs através de um grafo para auxiliar biólogos na exploração e na descoberta de conhecimento sobre estas seqüências. A metodologia inclui agrupamento usando um programa montador de seqüências e, conseqüentemente, a transformação dos grupos em nós de um grafo. O algoritmo BLAST é usado para procurar alinhamentos entre seqüências, representando-os posteriormente por arestas entre as seqüências mais similares. Para a visualização do grafo utilizamos e modificamos a ferramenta TG WikiBrowser conectada a um banco de dados. O resultado é uma ferramenta interativa baseada em código livre e robusto que funciona em ambientesWindows e Linux. Ela possibilita a fácil exploração do grafo, com diversas funcionalidades como, por exemplo: a expansão e filtragem do grafo, a busca por rótulos ou trechos de seqüências e a visualização detalhada de seqüências e grupos de seqüências. Com isso, os biólogos e especialistas em bioinformática ganham mais uma alternativa de investigação da genéticaExpressed Sequence Tags (ESTs) are samples of gene stretches, which play the role of templates in synthesis of proteins. Since the amount of collected ESTs on the past few years is enormous, the use of computers has become essential to fields like gene and protein identification, and gene homology. This work proposes a methodology and a tool for visualization of ESTs as a graph for aiding biologists on exploration and on knowledge discovery about these sequences. The methodology includes clustering of ESTs using an assembly program and, consequently, the transformation of the groups in nodes of a graph. BLAST algorithm is used to search alignments among sequences, later representing them as edges between the most similar sequences. For the graph visualization, we adapted TGWikiBrowser software connected to a database. The result is a robust and open source interactive tool forWindows and Linux. It allows easy graph exploration, with various functionalities, for example: graph expansion and filtering, searching for label or sequence stretches, and detailed visualization of sequences and groups of sequences. Therefore, we hope biologists can count on one more option in genetics research
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Mierzwa, Christof. "Architektur eines ESBs zur Unterstützung von EAI Patterns." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-35102.
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Kadlecová, Barbora. "Analýza přeshraniční spolupráce Euroregionu Nisa prostřednictvím EGTC -- ESÚS." Master's thesis, Vysoká škola ekonomická v Praze, 2010. http://www.nusl.cz/ntk/nusl-71964.
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Thesis in its theoretical part deals with cross-border cooperation and analyzes a new legal instrument at EU level - European Grouping of Territorial Cooperation (EGTC). The work describes the instrument, including the establishment of a legal basis. It also deals with the potential use of the EGTC, which focuses on the permanent structure for cross-border cooperation, ie. Euroregions and make a summary of the EGTC already developed within the European Union. The practical part focuses on a description of the Euroregion Neisse, who ordered the creation of a legal entity as one of the main objectives and is currently in the process of preparing the establishment of an EGTC. This section is in addition to the characteristics of the Euroregion analysis of individual steps in the creation, use specific options on the EGTC and the comparison with existing legal entities within the EU. The aim of the thesis is to analyze the EGTC and determine whether it has the potential for the development of the Euroregion Neisse.
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Fryer, Timothy James Osborne. "ESS models of sperm competition." Thesis, University of Sheffield, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266803.
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Calderón, Verástegui Ligia. "Esas acciones que nos sostienen: la naturaleza como "otro"." Canalé, 2012. http://repositorio.pucp.edu.pe/index/handle/123456789/114085.
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Marchand, Cécile. "Todas esas muertes de Carlos Droguett : genèse d'un texte." Poitiers, 2000. http://www.theses.fr/2000POIT5022.
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A Valparaiso, en 1905, un émigrant français, Emile Dubois, est exécuté bien qu'il clame son innocence dans la série d'assassinats dont on l'accuse. A partir de ce fait divers, l'écrivain chilien Carlos Droguett écrit un feuilleton "Dubois, artista del crimen", publié dans le journal "Extra" de 1946 à 1947. Vingt-quatre ans plus tard, il obtient le prix Alfaguara pour le roman "Todas esas muertes", qui reprend et développe le feuilleton précédemment cité. C'est précisément à la genèse de ce roman que s'attache cette thèse dont la particularité est de présenter le texte du feuilleton en regard du texte romanesque. Dans un premier temps, le processus de fictionnalisation du fait divers, opéré par l'auteur chilien, nourrit notre propros qui s'attache alors à l'étude des sources utilisées. L'analyse porte ainsi sur la presse contemporaine de l'événement qui a inspiré ces fictions, plus précisément sur le quotidien "El Mercurio de Valparaíso", mais surtout sur une biographie romancée que nos recherches ont mise au jour, "La verdadera historia de Dubois. Las Memorias del célebre criminal", afin de déterminer l'influence de ces sources sur les oeuvres de Carlos Droguett en question. Puis l'étude de la genèse de "Todas esas muertes" se poursuit à travers l'annotation du texte du roman, comparé à celui du feuilleton, par la mise en évidence des différences actantielles, thématiques et linguistiques qui apparaissent entre les deux oeuvres.
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Hanson, Jessi, Patrick Seeco Faley, and Megan Quinn. "Analysis of the Liberian Ebola Survivors Support System (ESSS)." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/6786.
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A systems theoretical analysis to capture the evolution and transition of the network systems supporting Ebola survivors and their affected communities, during the 2014-15 Ebola outbreak and recovery phases. The qualitative analysis includes a literature review, archival review, and interviews with representatives of key actors operating in strategic action fields. This paper uses a series of Diagrams that visually illustrate the various complex phases and their network changes that occurred and were established during the outbreak. This case analysis provides crucial phase information that both captures the historical events that informed the systems changes, including the development of the Ebola Survivors’ Support System (ESSS). Secondly, this analysis acts as, a model of understanding how disease support networks first emerge and can be better supported in other outbreaks.
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Frangou, Andreas John. "ESAS : towards a case-based intelligent system for competitive advantage." Thesis, University of Portsmouth, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310417.
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Quiñones, Patricia. "Reflexiones sobre esos “otros extraños” liberales en la sociedad holandesa." Anthropía, 2014. http://repositorio.pucp.edu.pe/index/handle/123456789/78188.
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Holanda es considerada como un modelo de sociedad liberal. Sin embargo, el sistema liberal presenta contradicciones al verse enfrentado con nuevas categorías de “los otros” y sus efectos en la identidad holandesa. El caso desarrollado en este artículo es el uso de velo de las mujeres musulmanas y como se presentan las inconsistencias del modelo liberal en una sociedad multicultural.
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Vainutytė, Raminta. "Kultūrinių plakatų serija Matai, čia esu aš." Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140717_110330-80655.
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Mano bakalauro darbas vadinasi “Matai, čia esu aš”. Šio darbo tikslas – panaudojant šiuolaikines išraiškos priemone ir technologiją įdomiai pateikti mane dominusią problemą: aktoriaus menamo personažo ir paties aktoriaus asmenybės sutapatinimas žiūrovo sąmonėje. Kuris yra tikrasis? Kuriuo lengviau būti? Ką apskritai reiškia būti savimi? Šiuo darbu noriu atkreipti mūsų dėmesį į tai, kad toks sutapatinimas neskatina žiūrovo kritiškai mąstyti, o aktorius būna labai destruktyvus, pavyzdžiui, skaudus V. Šapranausko atvėjis.
Temą pateikti pasirinkau reklaminio plakato formą. Tačiau formatas nestandartinis – trys plakatai 70x290 (cm), atspausta ant audinio. Tai yra mano fotografuoti konkrečių aktorių portretai. Dalis veido yra aktorius realiame gyvenime, dalis su jo kuriamo personažo grimu. Papildoma priemonė dviprasmiškumui pabrėžti – dalinamas į spalvotą ir nespalvotą dalis. Portretai yra papildomi C. Chandler “Aš, Fellini” knygos mintimis apie aktoriaus darbą ir pateikiama pagrindinė darbo žinutė “Matai, čia esu aš”. Tokį plakatų dydį ir technologiją pasirinkau todėl, kad yra numatyta plakatus eksponuoti skirtingose aplinkose. Didelis mastelis tam, kad plakatai taptų ryškiu aplinkos akcentu, o atspaudas ant audinio pasirinktas tam, kad būtų lengva transportuoti ir eksponuoti.
Atlikdama šį darbą turėjau pademonstruoti gebėjimus pabrėžti problemą, pasirinkti išraiškias priemones, labiausiai atitinkančias mano situacijoje. Sukauptas žinias ir praktiką galima pritaikyti mokykloje... [toliau žr. visą tekstą]My Bachelor’s final thesis is titled "Look this is me". The aim - use of modern means of expression and technology to give perception of this problem: the character of an actor and the actor's personal identification in the minds of the viewer. Which is true? Which is easier to be? What does it mean to be true yourself? This work is trying to draw our attention to the fact that such identification does not encourage the viewer to think critically, and the actor is very destructive like in such severe case as V. Šapranauskas. This topic was chosen to be presented in a promotional poster form: non-standard format - three posters 70x290 (cm), printed on fabric. Portraits of specific actors were used from my personal photo shoot. Half of an actor’s face is from a real life and the other part is made with a makeup which represents its character. An additional measure to emphasize the ambiguity – photo division into colored and black and white parts. These portraits has added thoughts from a book of C. Chandler, "I, Fellini" and the main thesis message "Look this is me". The exact size and technology was chosen because this poster is supposed to be exhibited in different environments and places: a large scale in order to make posters bright and ambient accent and the printing on the fabric - for an easy transportation and display. While working on this thesis I had to demonstrate the ability to highlight the problem, to choose the means of expression most relevant to my situation... [to full text]
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Elemam, Aboubker. "Development of a ESES Solar Thermal Lab on Full Scale System." Thesis, Högskolan Dalarna, Maskinteknik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:du-12581.
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The main aim of this project is to develop an ESES lab on a full scale system. The solar combisystem used is available most of the time and is only used twice a year to carry out some technical courses. At the moment, there are no other laboratories about combisystems. The experiments were designed in a way to use the system to the most in order to help the students apply the theoretical knowledge in the solar thermal course as well as make them more familiar with solar systems components. The method adopted to reach this aim is to carry out several test sequences on the system, in order to help formulating at the end some educating experiments. A few tests were carried out at the beginning of the project just for the sake of understanding the system and figuring out if any additional measuring equipment is required. The level of these tests sequences was varying from a simple energy draw off or collector loop controller respond tests to more complicated tests, such as the use of the ‘collector’ heater to simulate the solar collector effect on the system. The tests results were compared and verified with the theoretical data wherever relevant. The results of the experiment about the use of the ‘collector’ heater instead of the collector were positively acceptable. Finally, the Lab guide was developed based on the results of these experiments and also the experience gotten while conducting them. The lab work covers the theories related to solar systems in general and combisystems in particular.
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Teixeira, Laura Helena Marcon. "Mapeamento funcional em cana-de-açucar utilizando ESTs como marcadores moleculares." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316499.
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Orientadores: Anete Pereira de Souza, Luciana Rossini PintoDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A cana-de-açúcar (Saccharum spp.) está entre as espécies de maior importância econômica no mundo, constituindo uma das principais fontes de produção de açúcar e álcool. Apesar de o Brasil ocupar posição de destaque, como o maior produtor mundial, os níveis de produtividade são considerados baixos. A obtenção de novas variedades de cana-de-açúcar, mais produtivas e resistentes a pragas e doenças, é um processo que consome vários anos até o lançamento para o plantio comercial. O desenvolvimento de mapas de ligação pode contribuir significativamente para os programas de melhoramento, principalmente na localização de genes associados a características agronômicas de interesse. Os bancos de dados de seqüências expressas (ESTs-database) oferecem uma oportunidade para a construção de mapas funcionais, os quais servem de base para a estratégia de genes candidatos (Candidate-gene approach). O projeto de seqüenciamento de ESTs (SUCEST) do programa Genoma da FAPESP já identificou cerca de 40 mil clusters que representam os genes de cana-de-açúcar. Deste modo, o mapeamento de ESTs pode ser conduzido pela análise do polimorfismo no comprimento de restrição (RFLPs) utilizando os ESTs como sondas de hibridização. Tendo em vista os avanços que serão alcançados no melhoramento genético da cana-de-açúcar com a exploração das informações contidas nos bancos de dados de ESTs, este projeto teve como objetivo mapear ESTs relacionados a genes de interesse em cana-de-açúcar, utilizando-os como sondas em ensaios de RFLP em uma progênie derivada do cruzamento entre duas variedades précomerciais de cana-de-açúcar. Assim é importante ressaltar que o presente projeto complementa um programa de mapeamento genético molecular de duas variedades pré-comerciais de cana-de-açúcar, e outro, de mapeamento de QTL¿s associados a características de interesse agronômico, utilizando como marcadores as seqüências produzidas pelo projeto SUCEST
Abstract: Sugarcane (Saccharum spp.) is the species that has the greatest economic importance in the world, as it is one of the main sources of sugar and alcohol production. Although Brazil is the biggest producer of this crop - participating with 25% of world production, the productivity levels are considered low. The acquisition of new sugarcane varieties, which are more productive and resistant to plagues and illnesses, is a lengthy process that takes years until the launching of the commercial crops. Linking map development can contribute significantly towards improvement programs, mainly in the localization of genes associated to the agronomical traits of interest. The expressed sequence tag (ESTs) database offers a chance for the construction of functional maps, which serve as a base for the Candidate-gene approach. The EST sequence project (SUCEST) of the FAPESP Genome program has already identified about forty thousand clusters that represent sugarcane genes. In this way, EST mapping can be led by the restriction fragment length polimorfism (RFLPs) analysis using the ESTs as hibridization probes. In view of the advances that will be reached in sugarcane genetic improvement with the exploration of the information contained in the EST database, the aim of this project is to map ESTs related to sugarcane interest genes using them as probes in the RFLP assays in a lineage derived from the crossing between two sugarcane commercial crosses. The present project complements a molecular genetic mapping program; and another QTL mapping, associates the agronomics traits, using the sequences produced for the SUCEST project as markers
Mestrado
Genetica Vegetal e Melhoramento
Mestre em Genética e Biologia Molecular
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Baudet, Christian. "Uma abordagem para detecção e remoção de artefatos em sequencias ESTs." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/276264.
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Orientador: Zanoni DiasDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Computação
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Resumo: O sequenciamento de ESTs (Expressed Sequence Tag) [2] e uma tecnica que trabalha com bibliotecas de cDNAs tendo como objetivo a obtençao de uma boa aproximaçao para o ?ndice genico, que e a listagem de genes existentes no genoma do organismo estudado. Antes da serem analisadas, as sequencias obtidas do sequenciamento dos ESTs devem ser processadas para eliminaçao de artefatos. Artefatos sao trechos que nao pertencem ao organismo ou que possuem baixa qualidade ou baixa complexidade. Trechos de vetores, adaptadores e caudas poli-A podem ser citados como exemplos de artefatos. A eliminaçao dos artefatos deve ser feita para que a an'alise das sequencias produzidas no projeto nao seja prejudicada por estes ?ru?dos?. Por exemplo, artefatos presentes em sequencias freq¨uentemente produzem erros em processos de clusterizaçao, pois eles podem determinar se sequencias serao unidas em um mesmo cluster ou separadas em clusters diferentes. Observando a importancia da realizaçao de um bom processo de limpeza das sequencias, o trabalho desenvolvido nesta dissertaçao teve como principal objetivo a obtençao de um conjunto eficiente de procedimentos de detecçao e remoçao de artefatos. Este conjunto foi produzido a partir de uma nova estrategia de deteçao de artefatos. Normalmente, cada projeto de seq¨uenciamento possui seu proprio conjunto de procedimentos dividido em varias etapas. Estas etapas sao, em geral, ligadas entre si e o resultado de uma pode influenciar o resultado de outra. A nossa estrategia visa a realizaçao destas etapas de forma totalmente independente. Alem da avaliaçao desta nova estrategia, o trabalho tambem realizou um estudo mais detalhado sobre dois tipos de artefatos: baixa qualidade e derrapagem. Para cada um deles, algoritmos foram propostos e validados atraves de testes com conjuntos de seq¨u?encias produzidas em projetos reais de sequenciamento. O conjunto final de procedimentos, baseado nos estudos desenvolvidos durante a escrita deste texto, foi testado com as sequencias do projeto SUCEST [100, 103, 113] e mostrou bons resultados. O clustering produzido com as sequencias processadas por nossos metodos apresentou melhores consistencia interna e externa e menores taxas de redundancia quando comparado ao clustering original do projeto
Abstract: Expressed Sequence Tag (EST) Sequencing [2] is one technique that works with cDNA libraries. It aims to achieve a good approximation for the gene index of an organism. Before analyzing the sequences obtained by sequencing ESTs, they must be processed for artifact removal. An artifact is a sequence that does not belong to the studied organism or that has low quality or low complexity. As example of artifacts, we have adapters, poly- A tails, vectors, etc. Artifacts removal must be performed because their presence can produce ?noises? in the sequencing project data analysis. For example, artifact can join two sequences in a same cluster inappropriately or separate them in two different clusters when they should be put together. Motivated by the sequence cleaning process importance, our main objective in this work was to develop an efficient set of procedures to detect and to remove sequence artifacts. Usually, each EST sequencing project has its own procedure set divided in many steps. These steps are, in general, linked and the result of one given step might influence the result of the next one. Our strategy was to perform each step independently assuring that any execution order of those steps would lead to the same result. Additionally to the new strategy evaluation, this work also studied detailedly two type of artifacts: low quality and slippage. For each one, algorithms were proposed and validated through tests with sequences of real sequencing projects. The final set of procedure, developed in this work, was evaluated using the sequences of the SUCEST project [100, 103, 113] and produced good results. The resulting clustering from our method has better external and internal consistency and lower redundacy rate than those produced by the SUCEST project clustering
Mestrado
Ciência da Computação
Mestre em Ciência da Computação
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Bergfjord, Helena, and Varg Elin Reimann. "Användningen av ESAS vid bedömning av palliativa patienters symtom : En litteraturstudie." Thesis, Karlstads universitet, Institutionen för hälsovetenskaper (from 2013), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-81643.
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Introduktion/Bakgrund: För att systematiskt kartlägga palliativa patienters symtom så rekommenderas att vårdpersonal använder sig av bedömningsinstrument. Ett av de rekommenderade bedömningsinstrumenten i Nationell vårdplan för palliativ vård, är Edmonton Symptom Assessment System, ESAS. Syfte: Syftet var att beskriva effekten som användningen av ESAS har vid palliativ vård. Metod: Denna litteraturstudie har genomförts i enlighet med Polit och Becks (2017) nio steg. Databaserna PubMed och CINAHL användes för att inhämta data som sedan kvalitetsgranskades enligt Polit och Becks (2017) granskningsmallar. Induktiv ansats användes. Studiens resultat utgjordes av 13 artiklar som i sin tur ledde till fyra kategorier. Resultat: ESAS kunde identifiera flera symtom, medförde att symtom inte förbisågs och påvisade symtomkluster. Instrumentet var ett samtalsstöd och medverkade till en mer personcentrerad vård. ESAS träffsäkra symtombedömning leder till mer personcentrerad vård. Fyra kategorier identifierades; “Öppnar upp för samtal,” “Personcentrerad symtombedömning,” “Stöd vid symtomhantering och fördelning av resurser” och “Förbättrad livskvalitet.” Slutsats: ESAS erbjuder systematik vid bedömningar, vid uppföljning av symtom samt vid utvärdering av interventioner. Användningen av ESAS minskar risken för att förbise symtom. Bedömningar leder till tidigare implementering att palliativa behandlingar samt reducerar risken att utveckla långvariga problematiska symtom. Bedömningar med ESAS kan leda till en mer skräddarsydd symtomhantering och ökad livskvalitet.
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Yim, Cheuk-hon Terence. "Approximate string alignment and its application to ESTs, mRNAs and genome mapping." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31455736.
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Yim, Cheuk-hon Terence, and 嚴卓漢. "Approximate string alignment and its application to ESTs, mRNAs and genome mapping." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31455736.
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Edger, Patrick P. "Comparative analyses of floral gene sequences and ESTs from Tripsacum dactyloides L." Virtual Press, 2006. http://liblink.bsu.edu/uhtbin/catkey/1348861.
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In Tripsacum dactyloides, an ancestor of modem maize, genes that control floral development have not been described. A research program to identify and analyze the genes that control and regulate floral development in Tripsacum, has been undertaken through the bioinformatics analysis of Tripsacum cDNA libraries and suppression subtractive EST libraries of stage- and developmentally- specific Tripsacum floral tissue. Comparative analysis of nucleotide sequence data has revealed numerous genes previously described within other floral genomes such as Oryza sativa (rice), Zea mays (maize), Arabidopsis thaliana (thale cress), Triticum spp. (wheat) and Rosa rosesum (rose) well as unique gene sequences. Attempts have been made to characterize the unique genes through analysis of conserved domain sequence segments.Department of Biology
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Silva, Marcicleide Lima da. "Estudo de genes expressos em frutos de camu-camu: seqüenciamento de ests." Universidade Federal do Amazonas, 2006. http://tede.ufam.edu.br/handle/tede/4385.
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CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
The camu-camu (Myrciaria dubia (H.B.K.) McVaugh) is a native sort of the Amazonian region, whose fruit presents elevated content of ascorbic acid (vitamin C). The study of the functional genome in camu-camu fruits has like base the expressed sequence tags sequencing - ESTs. Faced with the displayed the present thesis is going to analyze and identify express genes in camu-camu fruits by means of ESTs sequencing. The total RNA was extracted from the shell-pulp. The extremity 5’sequencing' of cDNA insert was carried out so much in the Technology of the DNA Laboratory (UFAM) and in the Sequencing Platform (EMBRAPA/CENARGEN). The ESTs sequences obtained were submitted to the System Genome, program of genomic annotation that integrates analysis management programs and viewing of nucleotides sequences. It developed an efficient procedure for total RNA extraction of camu-camu fruits that enabled the obtaining of mRNAs of quality, utilized in the making of cDNAs of sizes varied (500pb to 4Kb). From the sequencing were obtained 3196 ESTs valid, being formed 1546 singletons and 358 contigs, resulting of 2586 ESTs sequences in total with similarity the sequences found in the gene bank. The analysis library clusterization revealed an index of 81% novelty and 32,54% redundancy. Around 90% of the contigs presented decrease redundancy (2-4 reads by contigs). The facts of the categorization of the proteins identified detached the posttranslational modification, protein turnover, chaperones (13,2%) category. From the hoist of the species with bigger number of ESTs with similarity the camu-camu sequences detached itself Arabidopsis thaliana with 49%. Around 10 uniques presented very high similarity (and-value 0.0) to known genes. The ESTs more abundantly express in camu-camu fruits encode to gluthatione s-transferase. They were observed around 3% sequences (97 ESTs) with decrease similarity (e-value > e-10) and 15% did not they present similarity with no contained sequence in the gene bank. They were identified 138 ESTs sequences (4,3%) that they encode molecular chaperones with prevalence of the sHSP family that represents 33% of express chaperones. ESTs related to the ascorbic acid metabolism also were identified, being nine related the synthesis and six come back for ascorbic acid conversion and recycling. ESTs related to the ripening and mechanisms of defense of the fruit also were noticeable.
O camu-camu (Myrciaria dúbia (H.B.K.) McVaugh) é uma espécie nativa da região Amazônica, cujo fruto apresenta elevado teor de ácido ascórbico (vitamina C). O estudo do genoma funcional em frutos de camu-camu tem como base o seqüenciamento de fragmentos de seqüências expressas - ESTs (Expressed Sequence Tags). Diante do exposto a presente tese pretende analisar e identificar genes expressos em frutos de camu-camu por meio de seqüenciamento de ESTs. O RNA total foi extraído a partir da casca-polpa. O seqüenciamento da extremidade 5’ de insertos de cDNA foi realizado tanto no Laboratório de Tecnologia do DNA da UFAM e como na Plataforma de Seqüenciamento da EMBRAPA/CENARGEN. As seqüências ESTs obtidas foram submetidas ao Sistema Genoma, programa de anotação genômica que integra programas de gerenciamento de análise e visualização de seqüências nucleotídicas. Os resultados obtidos foram o desenvolvimento de um procedimento eficiente para extração de RNA total de frutos de camu-camu que possibilitou a obtenção de mRNAs de qualidade, utilizados na confecção de cDNAs de tamanhos variados (500pb a 4Kb). A partir do seqüenciamento foram obtidas 3196 ESTs válidas, sendo formados 1546 singletons e 358 contigs, resultando num total de 2586 seqüências ESTs com similaridade a seqüências encontradas no banco de genes. A análise da clusterização da biblioteca revelou um índice de 81% de novidade e 33% de redundância. Cerca de 90% dos contigs apresentaram baixa redundância (2-4 reads por contigs). Os dados da categorização das proteínas identificadas destacaram a categoria modificação pós-traducional, proteína turnover, chaperonas (13,2%). A partir do levantamento das espécies com maior número de ESTs com similaridade a seqüências de camu-camu destacou-se Arabidopsis thaliana com 49%. Cerca de 10 uniques apresentaram altíssima similaridade (e-value 0.0) a genes conhecidos. Os ESTs mais abundantemente expresso em frutos de camu-camu codificam a glutationa s-transferase. Foram observados cerca de 3% de seqüências (97 ESTs) com baixa similaridade (e-value > e-1010) e 15% não apresentaram similaridade com nenhuma seqüência contida no banco de genes. Foram identificadas 138 seqüências ESTs (4,3%) que codificam chaperonas moleculares com destaque à família sHSP que representa 33% das chaperonas expressas. ESTs relacionados ao metabolismo do ácido ascórbico também foram identificados, sendo nove relacionados a síntese e seis voltados para conversão e reciclagem do ácido ascórbico. ESTs relacionados ao amadurecimento e mecanismos de defesa do fruto também foram destacados.
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Sousa, Rodrigo Guarischi Mattos Amaral de. "Abordagem Computacional para Identificar Novos SNVs em Bases de Dados de ESTs." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-23042013-082406/.
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Indivíduos não relacionados apresentam apenas 1% de diferenças entre seus genomas. Estas variações ocorrem na forma de substituições, inserções, deleções, rearranjos complexos ou até estruturais. Dentre essas variações, aquelas que apresentam uma frequência populacional acima de 1% são denominadas de polimorfismos. Tais variações são responsáveis por diferenças que vão desde a resposta imunológica até o tratamento com drogas, incluindo sensitividade das células tumorais, níveis de plasma, efeitos colaterais e toxicidade. A forma mais comum de polimorfismo genético entre humanos são os polimorfismo de base única ou Single Nucleotide Polymorphisms (SNPs), sendo mais de 47 milhões descritos no dbSNP, um banco de dados de pequenos polimorfismos do NCBI. No presente estudo, foi estabelecida uma abordagem computacional, com etapas de exclusão de regiões parálogas ou de baixa qualidade, com o objetivo de identificar variantes genéticas em sequências expressas gerados pelo método de Open Reading Frame ESTs (ORESTES) durante o Projeto Genoma Humano do Câncer. Diferentemente de outros softwares de detecção de polimorfismos, a abordagem computacional descrita neste estudo leva em consideração a informação a priori do número de bibliotecas distintas que reportaram a mesma variação. Foram identificadas 1900 mutações (853 sinônimas e 1047 não-sinônimas) presentes em duas ou mais bibliotecas distintas, que foram validados in-silico contra o dbSNP v130. O resultado da análise identificou 901 mutações já descritas no dbSNP (47,42%). Para confirmação da análise, foram selecionadas 10 mutações (6 novas e 4 já presentes no dbSNP) para validação pelo método de High Resolution Melt (HRM), seguido da caracterização por sequenciamento de DNA. Nesse caso, o resultado foi a validação de 50% das mutações selecionadas. A análise de interação protéica, Protein-Protein Interaction (PPI), realizada com as mutações não-sinônimas localizadas em domínios funcionais, revelou redes gênicas mais complexas em tecidos tumorais do que nos tecidos normais. Esta observação ratificou a literatura a respeito da transformação tumorigênica ser desencadeada pela combinação de mutações que ativam uma série de processos biológicos, para isso, afetando genes, vias gênicas e networks de vias gênicas relacionados. Em resumo, o presente estudo descreve uma abordagem computacional eficiente para identificação de mutações em dados de sequências expressas, além de avaliar o papel das mutações na tumorigênese.Unrelated humans have only 1% of non-simularity in their genome. These variations occur as substitutions, insertions, deletions, or even complex structural rearrangements. Among these variations, those which show a population frequency above 1% are called polymorphisms. Such variations are responsible for differences ranging from the immune response to treatment with drugs, including sensitivity of tumor cells, plasma levels, toxicity and side effects. The most common form of genetic polymorphism among human are Single Nucleotide Polymorphisms (SNPs), with more than 47 million reported in dbSNP, a database of small polymorphisms from NCBI. In this study, we established a computational approach, with steps to exclude low quality and paralogous regions, aiming to identify genetic variants in expressed sequences generated by the method of Open Reading Frame ESTs (ORESTES) for the Human Cancer Genome Project. Unlike other polymorphisms detection softwares, the computational approach described in this study takes into account the a priori information about the number of different libraries that reported the same variation. We identified 1900 mutations (853 synonymous and 1047 nonsynonymous) present in two or more different libraries, these mutations were in-silico validated against the dbSNP V130. The analysis result showed 901 mutations already described in dbSNP (47.42%). To confirm the analysis, we selected 10 mutations (six new and four already present in dbSNP) for validation by the method of High Resolution Melt (HRM), followed by characterization by DNA sequencing. In this case, the result was the validation of 50 % of the selected mutations. The Protein-Protein Interaction analysis (PPI), performed with non-synonymous mutations located in functional domains, showed more complex gene networks in tumor tissues than in normal tissues. This observation confirmed the literature regarding the tumorigenic transformation is triggered by the combination of mutations that activate a number of biological processes, thereby, affecting genes, gene pathways and networks of related gene pathways. In summary, this study describes an efficient computational approach to identify mutations in expressed sequence data, besides to evaluate the role of mutations in tumorigenesis.
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Snoeijer, Cristiane Quimelli. "Geração e análise de etiquetas de seqüências transcritas - ESTs - de Trypanosoma rangeli." Florianópolis, SC, 2004. http://repositorio.ufsc.br/xmlui/handle/123456789/88085.
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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-Graduação em BiotecnologiaMade available in DSpace on 2012-10-22T05:27:30Z (GMT). No. of bitstreams: 1 200174.pdf: 780725 bytes, checksum: 5105c9e6dff3c5b93f852902d1aee0fc (MD5)
O Trypanosoma rangeli, bem como o T. cruzi, são protozoários parasitas da Ordem Kinetoplastida sendo amplamente distribuídos nas Américas Central e do Sul, onde compartilham reservatórios, vetores em regiões geográficas distintas. Infecções produzidas pelo T. cruzi resultam na doença popularmente conhecida como mal de Chagas enquanto que as infecções causadas pelo T. rangeli parecem não ser patogênicas para seres humanos. Apesar disso, cerca de 60% da constituição antigênica solúvel destes parasitas é compartilhada o que pode determinar reações sorológicas cruzadas, dificultando o diagnóstico específico e mascarando a epidemiologia da doença de Chagas humana. As metodologias rotineiramente utilizadas no diagnóstico da doença de Chagas não são capazes de distinguir entre as duas espécies fazendo-se necessária a abertura de novas estratégias que nos permitam distinguí-las de maneira fácil, rápida e economicamente viável. No presente trabalho apresentamos os resultados obtidos à partir da construção e seqüenciamento de três bibliotecas de cDNA de formas epimastigotas da cepa Choachi de T. rangeli que resultaram na obtenção de 656 ESTs, dentre as quais apenas 20 ESTs foram homólogas à seqüências de T. rangeli e 245 não apresentaram homologia com seqüências dos bancos de dados pesquisados. Estes resultados demonstram a importância do uso deste tipo de estratégia para obtenção de novas informações à respeito do T. rangeli, servindo como base para a identificação de alvos diagnósticos ou para estudos da e suas interações com seus hospedeiros.
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Zhang, Li. "Study of FACTS/ESS Applications in Bulk Power System." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/28465.
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The electric power supply industry has evolved into one of the largest industries. Even though secure and reliable operation of the electric power system is fundamental to economy, social security and quality of modern life, the complicated power grid is now facing severe challenges to meet the high-level secure and reliable operation requirements.
New technologies will play a major role in helping today's electric power industry to meet the above challenges. This dissertation has focused on some key technologies among them, including the emerging technologies of energy storage, controlled power electronics and wide area measurement technologies. Those technologies offer an opportunity to develop the appropriate objectives for power system control.
The use of power electronics based devices with energy storage system integrated into them, such as FACTS/ESS, can provide valuable added benefits to improve stability, power quality, and reliability of power systems. The study in this dissertation has provided several guidelines for the implementation of FACTS/ESS in bulk power systems.
The interest of this study lies in a wide range of FACTS/ESS technology applications in bulk power system to solve some special problems that were not solved well without the application of FACTS/ESS. The special problems we select to solve by using FACTS/ESS technology in this study include power quality problem solution by active power compensation, electrical arc furnace (EAF) induced problems solution, inter-area mode low frequency oscillation suppression, coordination of under frequency load shedding (UFLS) and under frequency governor control (UFGC), wide area voltage control.
From this study, the author of this dissertation reveals the unique role that FACTS/ESS technology can play in the bulk power system stability control and power quality enhancement in power system. In this dissertation, almost all the studies are based on the real system problems, which means that the study results are special valuable to certain utilities that have those problems. The study in this dissertation can assist power industry choose the right FACTS/ESS technology for their intended functions, which will improve the survivability, minimize blackouts, and reduce interruption costs through the use of energy storage systems.Ph. D.
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Sales, Janice Silva. "Análise in silico em ests de cana-de-açúcar (Saccharum officinarum) relacionados à resistência ao estresse hídrico." Universidade Federal de Alagoas, 2006. http://repositorio.ufal.br/handle/riufal/190.
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The expressed sequenced tags (ESTs) generated by Sugarcane ESTs Project (FAPESP)
provided a useful databank for the investigation of DNA sequences related to many
different sugarcane agroindustrial features. When compared to DNA sequences from
several plant species available at The National Center for Biotechnology Information
Databank it was possible to perform a survey on various proteins produced by this plant
including those related to water stress resistance. SUCEST generated approximately 43,150
clusters from where 52 best hits were obtained in this survey for putative proteins related to
water stress resistance on 2,998 analyzed clusters. In silico protein identification was
achieved following a modified protocol developed by SUCEST which used specific
bioinformatics tools available at NCBi such as BLAST and FASTA.Fundação de Amparo a Pesquisa do Estado de Alagoas
Etiquetas de seqüências expressas geradas pelo Projeto ESTs de cana-de-açúcar (FAPESP) forneceram um banco de dados útil para a investigação das seqüências de DNA relacionadas a muitas características agroindustriais da cana-de-açúcar. Quando comparadas às seqüências de DNA de diversas espécies de plantas disponíveis no banco de dados do Centro Nacional de Informação Biotecnológica (NCBI), foi possível avaliar várias proteínas produzidas por esta planta, incluindo aquelas relacionadas à resistência ao estresse hídrico. O SUCEST gerou aproximadamente 43.150 clusters de onde foram obtidas 52 proteínas putativas relacionadas à resistência ao estresse hídrico em 2.998 clusters analisados. A identificação de proteínas in silico teve como suporte um protocolo modificado desenvolvido pelo SUCEST, que usou ferramentas de bioinformática específicas, disponíveis no NCBI, como por exemplo BLAST e FASTA.
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Kyriakopoulos, Charalampos [Verfasser], and Verena [Akademischer Betreuer] Wolf. "Stochastic modeling of DNA demethylation dynamics in ESCs / Charalampos Kyriakopoulos ; Betreuer: Verena Wolf." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2019. http://d-nb.info/1191755533/34.
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Rubio, Rubio Arelys. "Esos locos bajitos…: El Trabajo Doméstico en Niños y Adolescentes. Santiago, 1960-2005." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/110459.
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El tema de esta investigación lo constituye el trabajo doméstico, que diariamente han realizado miles de niños y jóvenes en nuestro país, en el silencio de sus hogares. Una primera aproximación con el contenido parte de los relatos que escuché desde niña, de boca de mi propia madre, quien, por circunstancias de la vida, desempeñó esta labor a muy corta edad, por lo tanto, mi principal motivación para llevarlo a cabo es adentrarme en mi historia familiar. Una segunda motivación está dada por mis recuerdos de infancia, transcurridos en una población donde en muchos hogares se vivían realidades semejantes, esto es, padres delegando las funciones caseras a los hijos para poder trabajar. Lo que me atrae, es, cómo, a través de la exploración de esta actividad tan ancestral, puede uno introducirse en la dinámica familiar, entender la intimidad de un hogar, llegando a comprender incluso la forma de ser de una persona en su interacción con el mundo. El trabajo doméstico es algo puntual, pero se desarrolla en el seno de las relaciones humanas, que reproducen y reflejan en un nivel micro a una sociedad. El quehacer hogareño entonces, entendido como historia de la familia, nos permite visualizar las mutaciones y continuidades que ésta ha sufrida en el tiempo y que dicen relación con cambios concretos en la estructura social. Por su parte, el trabajo doméstico infantil también ha manifestado aquellos cambios, como una respuesta adaptativa de las familias al entorno social.
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Nakano, Tokushige. "Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs." Kyoto University, 2014. http://hdl.handle.net/2433/185189.
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Andersson, Rönning Simon, and Jacob Rönning. "ESG-betyg och företagsvärdering : En studie om ESGs påverkan av en akties värdering." Thesis, Karlstads universitet, Handelshögskolan (from 2013), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-79061.
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Hållbarhet har blivit ett allt mer förekommande begrepp, idag kommunicerar de flesta företagen att de på ett eller annat sätt försöker bidra till en bättre och mer hållbar värld. Ett viktigt begrepp inom hållbarhet är ESG som står för miljö, socialt ansvar och bolagsstyrning. ESG bygger vidare på det äldre konceptet CSR som handlar om företagens ansvarstagande i samhället.Syftet med studie är att analysera om ett företags ESG-betyg har en signifikant påverkan på dess värdering, samt om ESG-betyget bidrar till en lägre eller högre värdering av ett företag. Företagens ESG-betyg kommer tillämpas som en sammanvägd variabel och som en uppdelad variabel efter miljö, socialt ansvar och bolagsstyrning. I studien behandlas 44 företag börsnoterade på OMX Stockholm Large Cap under perioden 2004 – 2019. För att mäta värderingen används olika nyckeltal som tar hänsyn till företagens börsvärde i relation till deras tillgångar eller intjäning.Resultatet visar att ett företags värdering påverkas i olika stor utsträckning av ESG-betyget beroende på vilket nyckeltal som tillämpas för att mäta värderingen. Det finns ingen regression där ESG har en signifikant påverkan på samtliga nyckeltal. Slutsatsen från analysen visar att ett bra ESG-betyg tenderar att dra upp värderingen, detsamma gäller när företag jobbar med socialt ansvar och bolagsstyrning. Fokus på miljö tenderar dock till att sänka värderingen för de regressionerna där ESG har haft en signifikant påverkan på värderingen.Sustainability has become a more and more recognized concept, today all the major corporations communicate that they are trying to contribute to a better and more sustainable world. An important term when it comes to sustainability is ESG which stands for Environmental, Social and Governance. ESG originates from CSR which stands for Corporate Social responsibility.This study aims to determine if ESG-rating have a significant impact on the valuation of a company, this study also aims to determine if the impact is positive or negative. The ESG-rating will be used as compounded variable and as variables divided into Environmental, Social and governance. This study consists of 44 companies listed on OMX Stockholm Large Cap between 2004-2019. In order to measure valuation, different key-ratios will be used, the key-ratios measure the market cap relative to different measures of assets and earnings.The result from this study shows that the impact ESG has on valuation varies depending on which key-ratios that are used in order to measure valuation. ESG does not have a significant impact on all the key-ratios. This study concluded that a high ESG-rating tend to increase valuation, that was also the case for Social and Governance. While high rating for environmental tend to decrease the valuation where the regression shows that ESG has a significant effect on the valuation.
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Goto, Kazuya. "Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors." Kyoto University, 2017. http://hdl.handle.net/2433/226763.
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Schleenstein, Dorothé. "Frauenspezifische Suchtproblematik aus theologischer Perspektive am Beispiel der Ess-Störungen /." Frankfurt am Main : Lang, 2010. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=018999763&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
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Schleenstein, Dorothé. "Frauenspezifische Suchtproblematik aus theologischer Perspektive am Beispiel der Ess-Störungen." Frankfurt, M. Berlin Bern Bruxelles New York, NY Oxford Wien Lang, 2009. http://d-nb.info/998696463/04.
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Stirnemann, Sandra. "Bedürfniswandel im Ess- und Verzehrverhalten der Schweiz Herausforderung, Erklärung, Konsequenzen /." St. Gallen, 2005. http://www.biblio.unisg.ch/org/biblio/edoc.nsf/wwwDisplayIdentifier/02601128001/$FILE/02601128001.pdf.
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Phuthi, Thabisani Nigel. "Steelmaking with the ESS furnace : a model-based metallurgical analysis." Diss., University of Pretoria, 2020. http://hdl.handle.net/2263/75934.
Full textAbstract:
This research project investigated the option of steelmaking with the ESS furnace by using computational
modelling to estimate steady state decarburisation rates. It focused on understanding metallurgical
phenomena that would dictate refining rates of molten pig iron with iron ore. The results obtained
are aimed at designers and potential users of the furnace technology to improve their understanding of
the expected steady-state process behaviour. A mass-and-energy-balance model with a decarburisation
sub-model was developed to estimate feed material requirements for steady state operation.
Modelling and simulation results suggest that it may not be possible to produce steel under the
conditions proposed. However, the furnace still holds potential if ideal operational conditions are understood
and applied.
Modelling also gave insight into which areas areas of concern, such as bubble formation in the furnace’s
channel induction heaters, and necessity for a well designed refractory lining to contain heat and allow the
process to operate at a stable condition under the conditions proposed.
Keywords: ESS furnace, steelmaking, metallurgical analysis, modelling, mass and energy balance,
decarburisation kineticsDissertation (MEng)--University of Pretoria, 2020.
Materials Science and Metallurgical Engineering
MEng
Unrestricted
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Almeida, Diego Dantas. "An?lise do transcriptoma das gl?ndulas venenosas do escorpi?o Tityus stigmurus." Universidade Federal do Rio Grande do Norte, 2011. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12593.
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Previous issue date: 2011-11-08Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
In Brazil, accidents with scorpions are considered of medical importance, not only by the high incidence, but also for the potentiality of the venom from some species in determining severe clinical conditions. Tityus stigmurus is a widely distributed scorpion species in Northeastern Brazil and known to cause severe human envenomations, inducing pain, hyposthesia, edema, erythema, paresthesia, headaches and vomiting. The present study uses a transcriptomic approach to characterize the molecular repertoire from the non-stimulated venom gland of Tityus stigmurus scorpion. A cDNA library was constructed and 540 clones were sequenced and grouped into 37 clusters, with more than one EST (expressed sequence tag) and 116 singlets. Forty-one percent of ESTs belong to recognized toxin-coding sequences, with antimicrobial toxins (AMP-like) the most abundant transcripts, followed by alfa KTx- like, beta KTx-like, beta NaTx-like and alfa NaTx-like. Our analysis indicated that 34% include other possible venom molecules , whose transcripts correspond to anionic peptides, hypothetical secreted peptides, metalloproteinases, cystein-rich peptides and lectins. Fifteen percent of ESTs are similar to cellular transcripts. Sequences without good matches corresponded to 11%. This investigation provides the first global view of cDNAs from Tityus stigmurus. This approach enables characterization of a large number of venom gland component molecules, which belong either to known or atypical types of venom peptides and proteins from the Buthidae family
No Brasil, acidentes com escorpi?es s?o considerados de import?ncia m?dicosanit?ria, n?o somente por sua incid?ncia, mas tamb?m pela potencialidade do veneno de algumas esp?cies em determinar quadros cl?nicos graves. Tityus stigmurus ? uma esp?cie de escorpi?o amplamente distribu?da na regi?o Nordeste do Brasil sendo conhecida por causar graves envenenamentos humanos, cujos sintomas incluem: dor, hipoestesia, edema, eritema, parestesia, cefal?ia e v?mitos. A composi??o qu?mica do veneno do escorpi?o T. stigmurus ainda n?o foi bem avaliada, havendo uma car?ncia na literatura de estudos com enfoque na elucida??o do repert?rio molecular dos componentes deste veneno. Neste trabalho, realizamos uma abordagem transcript?mica para caracterizar o repert?rio molecular da gl?ndula de veneno n?o-estimulada do escorpi?o Tityus stigmurus. Para tanto, uma biblioteca de cDNA foi constru?da e, ? partir dela, 540 genes foram clonados e agrupados em 37 clusters com mais de um EST (expressed sequence tag) e 116 singlets (somente um EST). Do total de transcritos, 41% pertencem a sequ?ncias de toxinas conhecidas, sendo os pept?deos antimicrobianos (AMPs) os transcritos mais abundantes, seguido por α-KTX, β- KTX, β-NaTx e α-NaTx, respectivamente. Nossa an?lise revelou ainda que 34% s?o "poss?veis toxinas", cujos transcritos correspondem a pept?deos ani?nicos, pept?deos hipot?ticos secretados, metaloproteases, pept?deos ricos em ciste?na e lectinas. Quinze por cento dos ESTs s?o semelhantes a transcritos celulares. Transcritos sem similaridade com outras sequ?ncias do GenBank, foram chamadas de no hit e correspondem a 11% do total. Este trabalho apresenta a primeira vis?o global de cDNAs das gl?ndulas venenosas do escorpi?o Tityus stigmurus. Esta abordagem permite a caracteriza??o de um grande n?mero de mol?culas componentes da gl?ndula de veneno, que pertencem a tipos conhecidos ou at?picos de pept?deos e prote?nas do veneno da fam?lia Buthidae
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Carmody, Carol Ann. "CLND and in-source CID ESMS : a route to a truly quantitative HPLC detector?" Thesis, University of Southampton, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401831.
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Gomes, Cicera Maria. "Análise do transcriptoma do fígado da serpente Bothrops jararaca utilizando expressed sequences tags (ESTs)." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-12062013-092051/.
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Bothrops jararaca é uma das principais serpentes responsáveis por acidentes ofídicos em São Paulo. O efeito do envenenamento pode ser local ou sistêmico, os quais são mediados por uma variedade de componentes do veneno. Considerando que, em animais vertebrados, o fígado desempenha atividades metabólicas essenciais, além de ser o principal órgão responsável pela síntese de proteínas do plasma, a obtenção do transcriptoma deste é de extrema importância para o estudo destas proteínas. Assim, o objetivo deste trabalho foi analisar o perfil de expressão de RNA no fígado de B. jararaca. Para isto foram obtidas 1700 sequências nucleotídicas denominadas Expressed Sequences Tags (ESTs). As sequências de nucleotídeos foram reunidas em 260 contigs, que foram submetidos ao banco de dados NCBI GenBank usando os algorítimos Blastx e Blastn. dos transcritos tiveram hit com banco de dados NR enquanto 30,5% das ESTs não tiveram homologia. De acordo com a análise do Gene Ontology (GO), os transcritos foram designados para processo biológico, componente celular e função molecular. A maioria dos transcritos foram agrupados em processo biológico, distribuídos em processo metabólico, processo celular e regulação biológica. No entanto, as atividades de ligação proteica, catalítica e de atividade regulatória enzimática foram as principais categorias relacionadas à função molecular. A análise do componente celular apresentou maior número de transcritos envolvidos com a parte celular, região extracelular e restrito a membrana de organela. O maior grupo de transcritos foi relacionado a inibidores de metaloproteases, inibidores de serinoproteases e inibidores de PLA2. Estudos de expressão gênica de alguns alvos selecionados na biblioteca de cDNA de B. jararaca foram realizados para comparação da expressão entre serpentes jovens e adultas. Esses resultados fornecem dados que poderão auxiliar nos estudos filogenéticos entre as diferentes espécies de serpentes e investigar a diferença no padrão da expressão gênica, fornecendo dados importantes sobre a biologia desses animais, contribuindo assim para a elucidação da fisiologia desses animais. Além disso, será útil na identificação de moléculas que possam ser candidatas a alvos terapêuticos.Bothrops jararaca is the main responsible for snake bites in São Paulo. There are both local and systemic envenomation effects, which are mediated by a variety of venom components. Considering that in vertebrate animals the liver is an important organ responsible for synthesis of plasma proteins, it would be valuable to get transcriptomic information about it. Thus, the aim of this work was to analyze expression profile at RNA level in B. jararaca liver. For this purpose, we sequenced 1700 Expressed Sequence Tags (ESTs) from a cDNA library of B. jararaca liver. Nucleotide sequences were assembled into 260 contigs, which were submitted to the GenBank NCBI database using BLASTX and BLASTN algorithms. Transcripts showed 43% hits with NR database while 30.5% of ESTs had no homology. According to Gene ontology (GO) analysis, transcripts were assigned for biological process, cellular component and molecular function. Majority of transcripts were classified in biological process category distributed in metabolic process, cellular processes and biological regulation, whereas binding and catalytic activities were the main category in molecular function. Cellular component analysis identified transcripts related to cell part, extracellular region and membrane-bounded organelle. The major group of transcripts was related to metalloproteinase inhibitors, followed by serine proteinase inhibitors and phospholipase A2 inhibitors. Studies of gene expression of some selected targets in the cDNA library of B. jararaca, by real time PCR were performed to compare expression in juvenile and adult snake specimens. Our results will help in studies of phylogenetic relationships between different snake species, and investigate differences in gene expression pattern. In addition, our findings are also helpful in the identification of active compounds for development of improved therapeutics for snake bites.
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Mahomed, Waheed. "Sequencing ESTs of the avocado transcriptome to study the tolerant response to Phytophthora cinnamomi." Diss., University of Pretoria, 2012. http://hdl.handle.net/2263/31150.
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Avocado (Persea americana Mill.) is an important crop whose cultivation is severely threatened
by Phytophthora cinnamomi Rands. The South African avocado industry makes an important
contribution to the world’s avocado supply, and is one of the world’s largest exporters. If the
current Phytophthora root rot problem is not addressed soon, the losses encountered by the
avocado industry may become so extensive that it results in job losses. The scant information
that is available for P. cinnamomi interaction studies indicate that there is no gene-for-gene
interaction yet described between the pathogen and host. Avocado genomics are not well
understood either and there is not much sequence data available for this basal angiosperm. The
data available comprises of sequence that was generated in marker studies on fruit and
flowering organs. It is now possible to generate large amounts of sequence data using highthroughput
sequencing platforms and identify defence-related genes. The identification of
defence-related genes in a tolerant rootstock will allow us to characterize the avocado-P.
cinnamomi interaction on a molecular level.
The aim of this MSc was to identify defence-related genes in a tolerant rootstock and
characterize their expression in order to understand the avocado-P. cinnamomi interaction.
Chapter 1 provides a comprehensive overview of the advances in molecular work
conducted on avocado thus far. A background of avocado rootstock development is provided
with details of molecular markers developed for use in avocado. Additionally, an introduction is
also given to high-throughput sequencing and its application to non-model crops such as
avocado. Chapter 2 describes the mRNA isolation and EST pyrosequencing of avocado roots.
Gene annotation of metabolic, cell wall associated and stress response genes are provided
along with the characterisation of defence-related genes. Chapter 3 reports of the expression profiling of defence-related genes obtained from
avocado root ESTs. The expression of nine defence-related genes are studied over six time
points in P. cinnamomi infected R0.09 tolerant avocado roots. Chapter 4 provides a general
discussion of the result obtained in this study along with future applications of the sequencing
data produced.Dissertation (MSc)--University of Pretoria, 2012.
Genetics
MSc
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Ponce, Alquicira Edith. "Food analyzing using electrospray mass spectrometry." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324538.
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Barron, Nicola-Jo. "A multiple criteria decision-making approach to establishing environmental and economic trade-offs in Pennine Dales agriculture." Thesis, University of Newcastle Upon Tyne, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339887.
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Fritzson, Sandra. "Innehållet i Magasinet och Ess i svenska utifrån styrdokument och betygskriterier." Thesis, Högskolan i Halmstad, Sektionen för lärarutbildning (LUT), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-17248.
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This study focuses on two text books, Magasinet and Ess i svenska, both common teaching material in Swedish schools. Since Swedish school system recently got a new curriculum and grading criteria, the purpose with this essay was to see how well the teaching materials used in school match the new documents. Since the subject has not really been studied before, the chapter containing recent studies focuses on layout, appearance and contents of good teaching material. The survey that I have implemented includes studies of the two text books, with comparison of the new curriculum, grading criteria and previous studies of what makes a good text book. Conclusions were that Magasinet is very comprehensive and match the criteria and curriculum very well, even though it spans over four classes and has to cover a lot of information. However, since it covers so much information, each section sometimes feels not as thorough as it perhaps should have been. Ess i svenska on the other hand, is a good complement to use in ninth grade, if you have studied the previous books for seventh and eight grade in the series. Though, with Ess i svenska on its own, it could be hard for the students to reach the passing grades since it sometimes lacks a proper description of the working process and does not cover all the criteria in the curriculum.
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Haapala, Linus, and Aleksander Eriksson. "RF High Power Amplifiers for FREIA – ESS : design, fabrication and measurements." Thesis, Uppsala universitet, Institutionen för fysik och astronomi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-263549.
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The FREIA laboratory is a Facility for REsearch Instrumentation and Acceleratior development at Uppsala University, Sweden, constructed recently to test and develop superconducting accelerating cavities and their high power RF sources. FREIA's activity target initially the European Spallation Source (ESS) requirements for testing spoke cavities and RF power stations, typically 400 kW per cavity. Different power stations will be installed at the FREIA laboratory. The first one is based on vacuum tubes and the second on a combination of solid state modules. In this context, we investigate different related aspects, such as power generation and power combination. For the characterization of solid state amplifier modules in pulsed mode, at ESS specifications, we implement a Hot Sparameter measurement set-up, allowing in addition the measurement of different parameters such as gain and efficiency. Two new solid state amplifier modules are designed, constructed and measured at 352 MHz, using commercially available LDMOS transistors. Preliminary results show a drain efficiency of 71 % at 1300 W pulsed output power. The effects of changing quiescent current (IDq) and drain voltage are investigated, aswell as the possibilities to combine several modules together.
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Benedetti, Florian. "Design of non-invasive profile monitors for the ESS proton beam." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS238/document.
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La source européenne de spallation (ESS) sera une infrastructure de recherche dévolue aux sciences utilisant les neutrons comme sonde d’observation. Elle est actuellement en construction à Lund, en Suède, et sera la plus brillante des sources de neutrons pulsées au monde. Comme son nom l'indique, la production des neutrons est assurée par les processus de spallation : des protons à haute énergie bombardant une cible de tungstène. Le faisceau de protons est généré par un puissant accélérateur linéaire de 2 GeV qui peut être divisé en deux parties : une partie "chaude" qui accélère les protons jusqu'à 90 MeV, suivie d’une partie « froide » constituée de cavités supraconductrices refroidies à l'hélium liquide, permettant d’atteindre les 2 GeV. La forte intensité de 62.5 mA et la longue impulsion de 2,86 ms répétée 14 fois par seconde, conduisent à une puissance moyenne de faisceau de 5 MW et une puissance crête de 125 MW. La connaissance du faisceau est donc indispensable pour la mise en service, c'est-à-dire le réglage du faisceau afin d'assurer un fonctionnement correct et sûr de la machine. Différents diagnostics seront installés le long de l'accélérateur pour remplir ces tâches.Cette thèse traite du développement d'un profileur transverse non invasif pour la partie froide de l’accélérateur de ESS : les Ionization Profile Monitors (IPM). La thèse se concentre sur les aspects critiques des IPM afin de s’assurer de leur faisabilité dans les conditions du faisceau de ESS. Ces moniteurs sont basés sur l’ionisation induite par le passage des protons du gaz résiduel présent dans le tube de l’accélérateur. Un champ électrique est appliqué entre deux plaques parallèles de l'IPM. Les électrons ou les ions dérivent vers un détecteur segmenté permettant de reconstruire le profil dans une direction transverse du faisceau.Plusieurs défis, qui auraient pu compromettre l’utilisation des IPM pour les mesures des profils de faisceau à ESS, sont décrits :• Les faibles taux de comptage dus aux faibles sections efficaces d'ionisation à haute énergie (90 à 2000 MeV) ainsi qu’aux basses pressions du gaz résiduel de l’ordre de 10-9 mbar,• L'homogénéité du champ électrique à l'intérieur de l'IPM, essentiel pour assurer des mesures de profils précises mais difficile pour les chambres à vide étriquées des IPM,• L’importante charge d'espace du faisceau, qui distord le profil mesuré en déviant lestrajectoires des produits d'ionisation. Cet aspect fondamental peut remettre en cause l’utilisation d’IPM pour faire des mesures fiables de profil de faisceau.Une fois ces études terminées, nous avons sélectionné trois systèmes de lecture fiables, basés sur :• des pistes conductrices lues par un intégrateur de charge multicanal,• des détecteurs à micro-canaux couplés à un écran phosphore (pMCP),• un détecteur de silicium développé au CERN, et utilisé en particulier pour le futur profileur du faisceau du PS.Ces études ont fait l’objet d’une Revue de Conception Préliminaire (PDR 2017/01) marquant le début de la construction des différents prototypes. Les tests préliminaires ont écarté la possibilité d'utiliser des détecteurs au silicium en raison des trop faibles énergies des ions incidents.En partant de zéro, des IPM, des moniteurs de référence et un banc d’essai ont été conçus et installés sur l’accélérateur de protons IPHI à Saclay. Les conditions expérimentales de ESS ont été reproduites afin de valider une solution pour les IPM, ainsi que tester nos modèles.Les campagnes de test ont montré qu'un MCP était nécessaire pour détecter le signal d’ionisation. De plus, l'IPM optique (pMCP + caméra) est la solution recommandée car elle offre une sensibilité plus élevée. Le retour d’expérience accumulé lors des tests des prototypes, nous a permis de proposer une conception quasi finale d’un IPM, présentée lors de la Revue Critique de Conception (CDR 2019/02), menant au début de la phase de productionThe European Spallation Source (ESS) will be a research infrastructure dedicated to sciences using neutrons as probes. The source is currently under construction in Lund, Sweden, and will be the world’s brightest pulsed source of neutrons. As its name suggests, the production of neutrons is ensured by the spallation process: high energy protons will impinge a tungsten target. To accelerate the protons, a powerful 2 GeV linear accelerator is being built. The accelerator can be split in two parts. A “hot” part is responsible for acceleration up to 90 MeV. Then a “cold” part made of superconducting cavities cooled with liquid helium is used to reach the highest energies. The high intensity of 62.5 mA and he long pulse of 2.86 ms repeated 14 times per second, lead to an incredible beam power of 5 MW in average and 125 MW in peak. The knowledge of the beam is therefore mandatory to ensure the commissioning, i.e. the beam tuning in order to achieve a proper and safe functioning of the machine. Different diagnostics will be installed along the accelerator to fulfil these tasks.This thesis deals with the development of a non-invasive transverse profiler for the cold part of the ESS accelerator: the Ionization Profile Monitor (IPM).The thesis focuses on critical aspects of the IPMs to guarantee its feasibility in ESS beam conditions. These monitors are based on the ionization of the residual gas induced by the proton beam inside the beam pipe. A transverse electrical field is generated between both parallel plates of the IPM. The electrons or ions drift, with respect to the electric field, towards a segmented detector allowing the reconstruction of the beam profile in one transverse direction. For a complete transverse profile, it is necessary to add a second profiler tilted by 90°.Several challenges for facing IPM to the ESS conditions, which may compromise their use, are described:• the weak counting rates due to the low ionization cross-sections at high energy (90 to 2000 MeV) and to the low residual gas pressure of 10-9 mbar,• the electric field homogeneity inside the IPM, which is relevant for insuring a precise profile measurement, was not obvious in the narrow vacuum chambers devoted to them,• the large Space Charge Effect of the beam, distorting the measured profile by deviating the ionization by-product trajectories. This fundamental aspect may compromise the use of an IPM for beam profile measurements.Once these former studies done, we selected the three reliable read-out systems based on:• conductive strips read by a multichannel charge integrator,• micro-channel plates coupled with phosphor screen (pMCP),• a silicon detector developed at CERN and foreseen for the future PS beam profiler.This work was the object of the Preliminary Design Review (PDR 2017/01) marking the beginning of the construction phase of the different prototypes. Preliminary tests discarded the possibility of using silicon detectors due to the low ion energies.Starting from scratch, IPMs, reference monitors and a test bench were designed and installed at the IPHI proton accelerator at Saclay. Close ESS conditions were achieved to validate an IPM solution and our simulations.The test campaigns showed that an MCP is mandatory to detect signal. Moreover, the optical IPM (pMCP + Camera) is the preferred solution since it provides higher sensitivity. Feedbacks from the prototype test campaigns, allows us to deliver an IPM final design presented during the Critical Design Review (CDR 2019/02) leading to the beginning of the production phase
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Hagemann, Sebastian [Verfasser]. "Debt Equity Swaps nach englischem und deutschem Recht unter besonderer Berücksichtigung des ESUG / Sebastian Hagemann." Baden-Baden : Nomos Verlagsgesellschaft mbH & Co. KG, 2014. http://d-nb.info/1108808425/34.
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Geirardsdottir, Kristin. "Identifying and analysing alternative splice variants by aligning ESTs and mRNAs to the genomic sequence." Thesis, University of Skövde, School of Humanities and Informatics, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-963.
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Questions have been raised about the genomic complexity of the human genome, since it was reported that it only consisted of 32,000 genes. Alternative splicing is considered the explanation of the enormous difference between the number of genes and the number of proteins. Aligning expressed sequence tags (ESTs) to the genomic sequence has become a popular approach for gene prediction, revealing alternative splice variants. The aim in this thesis is to identify and analyse splice variants of the adhesion family of G protein-coupled receptors using EST data. 75% of the genes in the data set of 33 sequences were found to have a total of 51 splice variants. About half of the variants were considered functional.
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SIMÕES, ALIANA PEREIRA. "ERGONOMIC EVALUATION OF THE USABILITY OF CEAD-IFES/ES’S VIRTUAL LEARNING ENVIRONMENT: A CASE STUDY." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2011. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=18087@1.
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O objetivo deste estudo foi realizar a avaliação ergonômica da usabilidade
de um ambiente virtual de aprendizagem (AVA) para demonstrar que a falta de
conhecimento dos benefícios da usabilidade e do ergodesign pode contribuir
com a baixa qualidade do ensino a distância. O ambiente virtual de
aprendizagem do CEAD/IFES-ES foi o objeto do estudo e para realizar esta
avaliação definiu-se os seguintes métodos e técnicas: aplicação do questionário
SUS, Avaliação Cooperativa e Avaliação Heurística. Os resultados obtidos com
esta avaliação apontaram que este ambiente apresenta graves problemas de
usabilidade. E, a partir destes resultados, foi possível gerar recomendações de
melhoria da usabilidade e do design da interface do ambiente. Espera-se que
este estudo possa auxiliar outras pesquisas que visam melhorar a qualidade dos
ambientes virtuais de aprendizagem.This study’s aim was carry through the ergonomic evaluation of the usability in a virtual learning environment to demonstrate how the lack of usability criteria and ergodesign can contribute with low quality of the distance education. The study’s object was CEAD/IFES-ES’s virtual learning environment and to conduct this evaluation the following methods and techniques were applied: SUS questionnaire, Cooperative Evaluation and Heuristic Evaluation. The obtained results pointed that this environment presents serious usability problems and showed that was possible to generate improvement recommendations for this interface environment with usability and design. There’s a hope that this scientific investigation can help other researches to improve the quality of virtual learning environments.
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Izacc, Silvia Maria Salem. "Análise da expressão gênica global durante a germinação do fungo aquático Blastocladiella emersonii." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-25042008-091322/.
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Foi realizado um programa de seqüenciamento de cDNAs obtidos de bibliotecas da fase de germinação do fungo aquático Blastocladiella emersonii. Os dados gerados, em conjunto com o projeto de seqüenciamento de cDNAs da fase de esporulação do fungo, permitiram a descoberta de aproximadamente 4.900 genes diferentes de B. emersonii (Ribichich et al., 2005). Os genes putativos foram anotados e associados com as categorias funcionais descritas pelo Gene Ontology Consortium e encontram-se na base de dados http://www.blasto.iq.usp.br. O perfil de expressão dos genes foi avaliado por Northern digital e vários transcritos apresentaram perfil de expressão regulado durante a germinação. Numa segunda etapa deste trabalho, cerca de 3.600 genes putativos de B. emersonii foram arranjados em lâminas de vidro e empregados como sondas para a investigação da expressão gênica global em células de diferentes tempos após a indução da germinação. Analisamos ainda, as diferenças entre a germinação induzida em meio nutriente e a germinação em solução inorgânica, na qual os indutores efetivos da germinação foram adenina ou íons potássio. Na germinação em meio nutriente mais de 900 genes, cerca de 26% do total presente nos micro-arranjos, mostraram-se diferencialmente expressos em pelo menos um dos tempos analisados. Foram induzidos durante a germinação, principalmente, genes envolvidos no crescimento celular, incluindo biossíntese de proteínas, transcrição e ativação do metabolismo energético. No entanto, estes genes não foram induzidos quando a germinação ocorreu em solução inorgânica. Verificamos ainda que vários transcritos envolvidos com a percepção do meio extracelular e com a sinalização celular, codificando proteínas necessárias para disparar o programa de germinação, encontram-se presentes nos zoósporos. Além disso, observamos que alguns dos transcritos encontrados nos zoósporos foram reprimidos na germinação em meio nutriente, mas mantiveram níveis elevados de expressão quando a germinação foi feita em solução inorgânica, indicando que os nutrientes exercem um papel importante na regulação da expressão de determinados genes nesta fase do desenvolvimento.We conducted large scale cDNA sequencing from libraries obtained using RNA from germinating cells of the aquatic fungus Blastocladiella emersonii. Data obtained in this work, along with cDNAs from sporulating cells, lead to the discovery of nearly 4.900 different genes from B. emersonii (Ribichich et al., 2005). The putative genes were annotated and associated with the functional categories described by the Gene Ontology Consortium and are available at the web site http://www.blasto.iq.usp.br. The expression patterns of these genes were evaluated by digital Northern analysis and we detected several transcripts that presented expression profile regulated during germination. In a second approach, nearly 3.600 putative genes from B. emersonii were spotted into glass slides and used as probes to investigate the global gene expression pattern in cells isolated at different times after induction of germination. We also analyzed the differences between the germination triggered in nutrient medium and the germination in inorganic solution, in which the effective inducers of germination were either adenine or potassium ions. More than 900 genes were differentially expressed during germination in nutrient medium in at least one of the time points analyzed, which correspond to 26 % of the total genes in the microarrays. The genes induced during this process were mainly those involved in cellular growth, including protein biosynthesis, transcription and energetic metabolism. However, these genes were not induced when germination occurred in inorganic solution. In addition, we verified that many transcripts involved in sensing the environment and also in signal transduction, encoding proteins necessary to trigger the germination program, were present in the zoospores. Another finding is that some of the transcripts found in zoospores were repressed when germination proceeded in nutrient medium, but were maintained at high levels when germination occurred in inorganic solution, suggesting that nutrients exert an important role in regulating the expression of some genes in this stage of development.
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Bergkvist, Alexander, Nils Hedberg, Sebastian Rollino, and Markus Sagen. "Surmize: An Online NLP System for Close-Domain Question-Answering and Summarization." Thesis, Uppsala universitet, Institutionen för informationsteknologi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-412247.
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The amount of data available and consumed by people globally is growing. To reduce mental fatigue and increase the general ability to gain insight into complex texts or documents, we have developed an application to aid in this task. The application allows users to upload documents and ask domain-specific questions about them using our web application. A summarized version of each document is presented to the user, which could further facilitate their understanding of the document and guide them towards what types of questions could be relevant to ask. Our application allows users flexibility with the types of documents that can be processed, it is publicly available, stores no user data, and uses state-of-the-art models for its summaries and answers. The result is an application that yields near human-level intuition for answering questions in certain isolated cases, such as Wikipedia and news articles, as well as some scientific texts. The application shows a decrease in reliability and its prediction as to the complexity of the subject, the number of words in the document, and grammatical inconsistency in the questions increases. These are all aspects that can be improved further if used in production.Mängden data som är tillgänglig och konsumeras av människor växer globalt. För att minska den mentala trötthet och öka den allmänna förmågan att få insikt i komplexa, massiva texter eller dokument, har vi utvecklat en applikation för att bistå i de uppgifterna. Applikationen tillåter användare att ladda upp dokument och fråga kontextspecifika frågor via vår webbapplikation. En sammanfattad version av varje dokument presenteras till användaren, vilket kan ytterligare förenkla förståelsen av ett dokument och vägleda dem mot vad som kan vara relevanta frågor att ställa. Vår applikation ger användare möjligheten att behandla olika typer av dokument, är tillgänglig för alla, sparar ingen personlig data, och använder de senaste modellerna inom språkbehandling för dess sammanfattningar och svar. Resultatet är en applikation som når en nära mänsklig intuition för vissa domäner och frågor, som exempelvis Wikipedia- och nyhetsartiklar, samt viss vetensaplig text. Noterade undantag för tillämpningen härrör från ämnets komplexitet, grammatiska korrekthet för frågorna och dokumentets längd. Dessa är områden som kan förbättras ytterligare om den används i produktionen.
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Padilha, Marcelo Henrique Peteres. "Fisiologia molecular digestiva de Musca domestica (DIPTERA)." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-10122009-135108/.
Full textAbstract:
A mosca domestica (Musca domestica) é um dos insetos largamente distribuído e conhecido pelo homem. A larva de M. domestica possui no conteúdo luminal do ventrículo anterior e médio uma atividade proteolítica com pH ótimo entre 3,0 3,5 e propriedades cinéticas similares a catepsina-D. Três cDNAs codificantes para preprocatepsina-D (ppCAD1, ppCAD2 e ppCAD3) foram clonados a partir de uma biblioteca de cDNA ventricular de larvas de M. domestica. As sequências possuem o peptídeo sinal, o propeptídeo e a enzima madura contendo os resíduos catalíticos e todos os resíduos de ligação ao substrato conservados, achados em uma catepsina-D lisossomal bovina. Um cladograma de sequências de aminoácidos de catepsinas-D de insetos e vertebrados depositados no GENBANK formou um grande grupo dividido em duas ramificações monofiléticas: Uma com sequências de vertebrados e a outra com sequências de catepsinas-D lisossomais de insetos incluindo a ppCAD1. A sequência do pepsinogênio humana, ppCAD2, ppCAD3 e uma sequência de D. melanogaster são excluídas desse grande grupo indicando uma função não lisossomal para essas sequências. ppCAD3 deve corresponder a uma catepsina-D digestiva encontrada no conteúdo luminal em larvas do inseto devido: (1) Análise por RT-PCR indicam que os transcritos codificantes para a CAD3 são expressos no ventrículo anterior e porção proximal do ventrículo médio. (2) pCAD3 recombinante após autoativação sob condições ácidas possui um pH ótimo entre 2,5 3,0 que é próximo ao pH luminal do ventrículo médio onde essa enzima atua. (3) Imunoblots das proteínas de diferentes tecidos e marcadas com o anticorpo preparado contra a pCAD3 foi positiva apenas nos tecidos e conteúdo do ventrículo anterior e médio. (4) CAD3 é localizada pela técnica de imuno-ouro no interior de vesículas de secreção e próxima a microvilosidades nas células do ventrículo anterior e médio. Esses dados suportam a idéia que ao se adaptar a um hábito detritivo, a CAD digestiva da mosca (e de outros Diptera Cyclorrhapha) resultou do mesmo gene ancestral da catepsina-D lisossomal intracelular, da mesma forma que se acredita que ocorreu com a pepsina em vertebrados. Uma limitada quantidade de informações de sequências de DNA e aspectos moleculares de M. domestica é disponível até o presente momento. Nós então propusemos gerar sequências de ESTs a partir de uma bibilioteca de cDNA ventricular da larva desse inseto. Um total de 826 ESTs randomicamente selecionados presentes no ventrículos de larvas de M. domestica foram seqüenciados e analisados com programas de bioinformática e separado em 323 clusters. As sequências foram manualmente anotadas e separadas em 3 categorias: (S) produtos provavelmente secretados, (H) produtos de metabolismo em geral (housekeeping) e (U) produtos sem função conhecida. Cento e sessenta clusters (423 ESTs) codificavam para proteínas secretadas tais como: lisozimas, lipases, tripsinas, quimotripsinas, dipeptidades, carboxipeptidase e α-amilases. Cento e trinta e dois clusters (190 ESTs) codificavam para sequências de metabolismo em energético, síntese de proteínas, transdução de sinal e outras funções celulares. Noventa e cinco clusters (231 ESTs) codificaram para proteínas sem similaridades com proteínas conhecidas no banco de dados. Estudos de expressão, localização e imunocitoquímica de sequências alvos deverão no futuro nos fornecer um melhor entendimento da fisiologia digestiva da larva de Musca domestica em detalhes moleculares. Uma enzima lipolítica (LipMD) foi identificada no item anterior. A sequência de aminoácidos obtidas é homóloga a lipases e contém os três bem conservados resíduos de aminoácidos que compõe a tríade catalítica (Ser183, His273 e Asp208) para esse grupo de enzimas. O fragmento de cDNA codificante para a LipMD foi clonado em vetor pAE (Ramos et al., 2004) e expresso em E. coli produzindo uma enzima com massa molecular de 37,3 kDa. A LipMD recombinante foi purificada e é capaz de hidrolizar tributirina e um grande número de substratos (pNP-acetato a pNP-esterato) e possui um pH ótimo próximo de 7,5. Analise por RT-PCR mostrou que os transcritos codificantes para a LipMD são expressos apenas no ventrículo anterior. Western-blots após SDS-PAGE das proteínas de vários tecidos e marcados com o anticorpo produzido contra a LipMD revelou a ocorrência da enzima principalmente no conteúdo luminal do ventrículo anterior. A LipMD é localizada pela técnica de imuno-ouro no interior de vesículas de secreção próximas a microvilosidades no interior das células do ventrículo anterior. Esta enzima pode atuar como uma enzima lipolítica digestiva secretada nessa região do ventrículo de larvas de M. domesticaThe house fly, Musca domestica is one the best known and most widely distributed insects known to humans. M. domestica larvae display in anterior and middle midgut contents, a proteolytic activity with pH optimum of 3.0-3.5 and kinetical properties like cathepsin-D. Three cDNAs coding for preprocatepsin D-like proteinases (ppCAD1, ppCAD2, ppCAD3) were cloned from a M. domestica midgut cDNA library. The sequences encoding the signal peptide, propeptide and mature enzyme having all conserved catalytic and substrate binding residues found in bovine lysosomal cathepsin-D. A cladogram of sequences of insect and vertebrate cathepsin-D-like proteinases deposited on GENBANK form a large grouping divided into two monophyletic branches: one with vertebrate and the other with insect lysosomal sequences including ppCAD1. Human pepsinogen, ppCAD2, ppCAD3, and a sequence from Drosophila melanogaster are excluded indicating a nonlysosomal function for them. CAD3 should correspond to the digestive CAD found in enzyme assays because: (1) The mRNA for CAD3 is expressed (RT-PCR) only in the anterior and proximal middle midgut. (2) Recombinant pCAD3, after auto activation has a pH optimum of 2.5-3.0 that is close to the luminal pH of M. domestica midgut. (3) Immunoblots of proteins from different tissues and stained with antiserum prepared against recombinant pCAD3 were positive only for the anterior and middle midgut tissue and contents. (4) CAD3 is localized with immunogold labeling inside secretory vesicles and around microvilli in anterior and middle midgut cells. The data support the view that on adapting to a detritivorous habit M. domestica digestive CAD (and of other Diptera Cyclorrhapha) resulted from the same archetypical gene as the intracellular cathepsin-D, paralleling what happened with vertebrates. A limited amount of data regarding DNA sequences and molecular aspects of Musca domestica species is avaliable. We proposed to generate ESTs sequences from a cDNA library constructed from larval midguts. A total of 826 randomly selected midgut derived cDNAs were sequenced and assembled based on their similarities into 323 clusters. The sequences were classified into three categories: (S) probably secretory products, (H) housekeeping products and (U) products with unknown cell localization and function. One hundred and sixty clusters (423 ESTs) encode putative secreted proteins such as lysozymes, lipases, trypsins, chymotrypsins, dipeptidases, carboxypeptidases A and α-amylases. One hundred and thirty two clusters (190 ESTs) encode housekeeping sequences associated with energy metabolism, protein syntesis, signal transduction and other cellular functions. Ninety five clusters (213 ESTs) encode proteins with no similarity with known proteins. Expression and high-throughput bioassay screening of target sequences must provide us with a better understanding of the digestive physiology of Musca domestica midgut larvae, in molecular detail. A lipolytic enzyme (LipMD) was identified from expressed sequence tags (EST) constructed from midgut larvae cDNA library. The deduced amino acid sequence is homologous to lipases and contained the three well-conserved amino acid residues, Ser183, His273, and Asp208, which form the catalytic triad of the enzyme. The cDNA fragment encoding for LipMD was cloned into a pAE vector (Ramos et al., 2004) and expressed in E. coli producing an enzyme with a molecular mass of 37.3 kDa. The recombinant LipMD was purified and was able to hydrolyse tributyrin and a broad range of substrates, from C2 to C18 p-nitrophenyl-esters and displayed an optimal pH of approximately 7.5. RT-PCR analysis in tissue homogenates (anterior, middle and posterior midguts, hemolymph, fat body and Malpighian tubules) showed that LipMD mRNA transcripts were expressed only in anterior midgut. Western-blots after SDS PAGE of proteins from different tissues and stained with anti-LipMD serum revealed that the enzyme occurs mainly in the anterior midgut lumen. LipMD is localized with immunogold labeling inside secretory vesicles and around microvilli in anterior midgut cells. LipMD is a candidate to be the digestive lipolytic enzyme found in that midgut region