Relevant bibliographies by topics / Ethidium bromide. eng

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Academic literature on the topic 'Ethidium bromide. eng'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 February 2022

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Journal articles on the topic "Ethidium bromide. eng"

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González-Villaseñor, Lucia Irene, Amanda M. Burkhoff, Víctor Corces, and Dennis A. Powers. "Characterization of Cloned Mitochondrial DNA from the Teleost Fundulus heteroclitus and its Usefulness as an Interspecies Hybridization Probe." Canadian Journal of Fisheries and Aquatic Sciences 43, no. 10 (1986): 1866–72. http://dx.doi.org/10.1139/f86-231.

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Analysis of mitochondrial DNA endonuclease restriction patterns is a powerful tool for studying related species and variation within species. The ethidium bromide staining technique has limited the number of digestions of mitochondrial DNA per individual. Because 32P-end-labeling also imposes severe limitations, we have resorted to cloning the fish (Fundulus heteroclitus) mitochondrial genome in the lambda replacement vector EMBL-3. The clone was used as a radioactive probe via Southern blotting to detect mitochondrial DNA restriction fragments obtained by multiple restriction endonuclease dig
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Iwata, M., D. Myerson, B. Torok-Storb, and R. A. Zager. "An evaluation of renal tubular DNA laddering in response to oxygen deprivation and oxidant injury." Journal of the American Society of Nephrology 5, no. 6 (1994): 1307–13. http://dx.doi.org/10.1681/asn.v561307.

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It has recently been suggested that endonuclease activation and/or apoptosis, possibly triggered by oxidant stress, are important pathogenetic mechanisms in oxygen deprivation/reoxygenation-induced proximal tubular cell death. To explore this possibility, DNA "laddering," a characteristic feature of these processes, was sought in: (1) postischemic rat kidneys (25- or 40-min arterial clamping; 0, 1, 4, 8, 24, and 48 h and 6 days reflow); (2) posthypoxic isolate rat proximal tubular segments and (3) cultured human kidney proximal tubular cells (HK-2) subjected either to energy depletion plus Ca2
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Biggiogera, M., and F. F. Biggiogera. "Ethidium bromide- and propidium iodide-PTA staining of nucleic acids at the electron microscopic level." Journal of Histochemistry & Cytochemistry 37, no. 7 (1989): 1161–66. http://dx.doi.org/10.1177/37.7.2471727.

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Ultra-thin sections of various tissues were stained with ethidium bromide or propidium iodide, two fluorescent markers widely used for quantitation of nucleic acids. The fluorochromes, tested at different concentrations, were then revealed by incubation of the sections with neutralized phosphotungstic acid. We showed that at the electron microscopic level only nucleic acid-containing structures are revealed. Chromatin, nucleolus, and ribosomes appear to be stained by the end-product of the reaction. Furthermore, controls with proteases and nucleases showed that the staining is related to the b
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Rouquette-Loughlin, Corinne, Steven A. Dunham, Michael Kuhn, Jacqueline T. Balthazar, and William M. Shafer. "The NorM Efflux Pump of Neisseria gonorrhoeae and Neisseria meningitidis Recognizes Antimicrobial Cationic Compounds." Journal of Bacteriology 185, no. 3 (2003): 1101–6. http://dx.doi.org/10.1128/jb.185.3.1101-1106.2003.

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ABSTRACT In Neisseria gonorrhoeae and Neisseria meningitidis, we identified a gene that would encode a protein highly similar to NorM of Vibrio parahaemolyticus (Y. Morita et al., Antimicrob. Agents Chemother. 42:1778-1782, 1998). A nonpolar insertional mutation in either the gonococcal or meningococcal norM gene resulted in increased bacterial sensitivity to compounds harboring a quaternary ammonium on an aromatic ring (e.g., ethidium bromide, acriflavine hydrochloride, 2-N-methylellipticinium, and berberine). The presence of point mutations within the −35 region of a putative norM promoter o
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Seitz, S., A. V. Schally, S. Gluck, et al. "Effective treatment of triple-negative breast cancer with targeted cytotoxic somatostatin analogue AN-162 (AEZS-124)." Journal of Clinical Oncology 27, no. 15_suppl (2009): 619. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.619.

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619 Background: Triple negative breast cancers (TNBC) represent a distinct subtype of breast cancer being negative to ER, PR and HER2 and are associated with poor prognosis. Limited systemic treatment options exist for TNBC. TNBC cells express somatostatin receptors (SSTR). Therefore, to investigate preclinical characteristics of TNBC we used a novel targeted cytotoxic somatostatin analogue AN-162 containing doxorubicin (DOX) which binds to the subtypes 2, 3 and 5 of SSTR. Methods: The expression of SSTR in HCC 1806 human TNBC cell line was detected by RT-PCR. Cytotoxic effect of AN-162 in vit
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Almasan, A., and N. C. Mishra. "Molecular characterization of the mitochondrial DNA of a new stopper mutant ER-3 of Neurospora crassa." Genetics 120, no. 4 (1988): 935–45. http://dx.doi.org/10.1093/genetics/120.4.935.

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Abstract An ethidium bromide-induced stopper mutant of Neurospora crassa is characterized at the molecular level. The mutant has two populations of mitochondrial DNA: a defective predominant mutant molecule and a basal level of the wild-type molecule. The aberrant DNA resulted after a 25-kbp deletion from the wild-type mitochondrial chromosome, which included major genes such as cytb, co1 and oli2. The deletion endpoints are located in the second intron of the ND5 gene, and in a sequence 250 nucleotides upstream of the co2 gene. The recombination has taken place between two nine nucleotide rep
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Shaker, O., H. Aboul Fadl, and M. K. El Hatw. "Prevalence of hepatitis C virus RNA in patients with chronic renal diseases." Eastern Mediterranean Health Journal 4, no. 3 (1998): 548–53. http://dx.doi.org/10.26719/1998.4.3.548.

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The prevalence of hepatitis C virus [HCV] RNA in 80 patients with chronic renal diseases was determined. Two sets of primers from the non-coding region of the hepatitis C virus were used. The products [188 bp] amplified by polymerase chain reaction were visualized by 2% agarose gel electrophoresis stained with ethidium bromide. The patients were classified into four groups. Group I comprised 40 adult patients with end-stage renal disease, 31 of whom were positive for HCV-RNA [77.5%] ; group II, 22 children with glomerulopathies, 15 of whom were positive [68.2%] ; group III, 9 children with chr
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Tsuge, Kenji, Yuichiro Ohata, and Makoto Shoda. "Gene yerP, Involved in Surfactin Self-Resistance in Bacillus subtilis." Antimicrobial Agents and Chemotherapy 45, no. 12 (2001): 3566–73. http://dx.doi.org/10.1128/aac.45.12.3566-3573.2001.

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ABSTRACT Surfactin is a cyclic lipopeptide biosurfactant. Transposon mutagenesis was performed in Bacillus subtilis strain 168, and a surfactin-susceptible mutant, strain 801, was isolated. Analysis of the region of insertion revealed that yerPwas the determinant of surfactin self-resistance. YerP had homology with the resistance, nodulation, and cell division (RND) family proton motive force-dependent efflux pumps only characterized in gram-negative strains. The yerP-deficient strain 802, in which the internal region of the yerP gene of B.subtilis strain 168 was deleted, showed susceptibility
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Jakeway, S. C., and U. J. Krull. "Consideration of end effects of DNA hybridization in selection of fluorescent dyes for development of optical biosensors." Canadian Journal of Chemistry 77, no. 12 (1999): 2083–87. http://dx.doi.org/10.1139/v99-200.

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Intercalating fluorescent dyes are in widespread use to detect the presence of double-stranded DNA. Applications include the development of biosensors that rely on the attachment ("tethering") of a dye molecule by a short hydrocarbon chain to the terminus of a strand of DNA so that dye is continuously available and the biosensor is fully reversible. Double strands of DNA have end effects that limit the stability of hybridization and dye intercalation near the termini of the duplexes. Therefore, the selection of the dye must be based on consideration of spectroscopic properties and also issues
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Nagy, Lajos, Romeo E. Morales, Martin Beinborn, Peter Vattay, and Sandor Szabo. "Investigation of gastroprotective compounds at subcellular level in isolated gastric mucosal cells." American Journal of Physiology-Gastrointestinal and Liver Physiology 279, no. 6 (2000): G1201—G1208. http://dx.doi.org/10.1152/ajpgi.2000.279.6.g1201.

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We tested the hypothesis that recognized gastroprotective agents exert direct protection against ethanol-induced injury in isolated rat gastric mucosal cells in vitro. If protection exists, we also wanted to identify subcellular targets in the reversible and/or irreversible stages of cell injury. Ethanol-induced cell injury was quantified by measuring plasma membrane leakage (trypan blue exclusion and lactate dehydrogenase release), mitochondrial integrity (succinic dehydrogenase), and nuclear damage (ethidium bromide-DNA fluorescence). Initial cell viability and responsiveness were estimated
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Dissertations / Theses on the topic "Ethidium bromide. eng"

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Fonseca, Janaína Conrado Lyra da. "Avaliação de métodos para tratamento de resíduos químicos originados em laboratórios biológicos /." Araraquara : [s.n.], 2006. http://hdl.handle.net/11449/105756.

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Resumo: Substâncias perigosas são freqüentemente utilizadas em laboratórios de ensino e pesquisa das Universidades. Até pouco tempo não existia preocupação com o destino destas substâncias após o uso. Tinha-se a falsa idéia de que como a Universidade utiliza poucas quantidades, de diferentes produtos, seu efeito ao ambiente era irrelevante. Felizmente atualmente este cenário é outro e as Universidades estão implementando programas de gerenciamento e em alguns poucos casos, de tratamento de resíduos. Diferentemente de agentes biológicos, substâncias químicas não possuem um procedimento de degra
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