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1

Steffan, Robert J., Joseph Quinnan, Matthew Walsh, Stewart H. Abrams, Simon Vainberg, Charles Condee, A. Paul Togna, and Paul B. Hatzinger. "IN SITU AND EX SITU APPROACHES FOR MTBE BIOREMEDIATION." Proceedings of the Water Environment Federation 2000, no. 10 (January 1, 2000): 225–36. http://dx.doi.org/10.2175/193864700784545432.

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2

Guerin, Turlough F. "Ex-situ bioremediation of chlorobenzenes in soil." Journal of Hazardous Materials 154, no. 1-3 (June 2008): 9–20. http://dx.doi.org/10.1016/j.jhazmat.2007.09.094.

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3

Lin, Ta-Chen, Po-Tsen Pan, and Sheng-Shung Cheng. "Ex situ bioremediation of oil-contaminated soil." Journal of Hazardous Materials 176, no. 1-3 (April 15, 2010): 27–34. http://dx.doi.org/10.1016/j.jhazmat.2009.10.080.

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4

Paul, Oindrila, Amrita Jasu, Dibyajit Lahiri, Moupriya Nag, and Rina Rani Ray. "IN SITU AND EX SITU BIOREMEDIATION OF HEAVY METALS: THE PRESENT SCENARIO." Journal of Environmental Engineering and Landscape Management 29, no. 4 (December 16, 2021): 454–69. http://dx.doi.org/10.3846/jeelm.2021.15447.

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Enhanced population growth, rapid industrialization, urbanization and hazardous industrial practices have resulted in the development of environmental pollution in the past few decades. Heavy metals are one of those pollutants that are related to environmental and public health concerns based on their toxicity. Effective bioremediation may be accomplished through “ex situ” and “in situ” processes, based on the type and concentration of pollutants, characteristics of the site but is not limited to cost. The recent developments in artificial neural network and microbial gene editing help to improve “in situ” bioremediation of heavy metals from the polluted sites. Multi-omics approaches are adopted for the effective removal of heavy metals by various indigenous microbes. This overview introspects two major bioremediation techniques, their principles, limitations and advantages, and the new aspects of nanobiotechnology, computational biology and DNA technology to improve the scenario.
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5

Wulandari, Andriyani Dea, and Vincentia Irene Meitiniarti. "Bioremediation of Pb and Cd contaminated soil using microorganism." Journal of Science and Science Education 5, no. 1 (September 22, 2021): 1–11. http://dx.doi.org/10.24246/josse.v5i1p1-11.

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The increase in industrial waste, especially those containing Pb and Cd, which is discharged into the environment results in more polluted environment conditions. Polluted enviornment is very dangerous for the survival of living things. This technique use living things to reduce environmental pollution, making it safe for living things. Bioremediation can be carried out by in-situ and ex-situ methods with several bioremediation mechanisms, including biosorption, bioaccumulation, bioleaching, and bioprecipitation. The use ex-situ techniques in bioremediation is easier to do, especially if it is carried out to remediate soils under controlled conditions in the laboratory.
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6

Sayqal, Ali, and Omar B. Ahmed. "Advances in Heavy Metal Bioremediation: An Overview." Applied Bionics and Biomechanics 2021 (November 11, 2021): 1–8. http://dx.doi.org/10.1155/2021/1609149.

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The pollution of toxic heavy metals is considered one of the most important environmental issues which has accelerated dramatically due to changing industrial activities. This review focuses on the most common methods, strategies, and biological approaches of heavy metal bioremediation. Also, it provides a general overview of the role of microorganisms in the bioremediation of heavy metals in polluted environments. Advanced methods of heavy metal remediation include physicochemical and biological methods; the latter can be further classified into in situ and ex situ bioremediation. The in situ process includes bioventing, biosparging, biostimulation, bioaugmentation, and phytoremediation. Ex situ bioremediation includes land farming, composting, biopiles, and bioreactors. Bioremediation uses naturally occurring microorganisms such as Pseudomonas, Sphingomonas, Rhodococcus, Alcaligenes, and Mycobacterium. Generally, bioremediation is of very less effort, less labor intensive, cheap, ecofriendly, sustainable, and relatively easy to implement. Most of the disadvantages of bioremediation relate to the slowness and time-consumption; furthermore, the products of biodegradation sometimes become more toxic than the original compound. The performance evaluation of bioremediation might be difficult as it has no acceptable endpoint. There is a need for further studies to develop bioremediation technologies in order to find more biological solutions for bioremediation of heavy metal contamination from different environmental systems.
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7

Wadgaonkar, Shrutika L., Alberto Ferraro, Yarlagadda V. Nancharaiah, Karaj S. Dhillon, Massimiliano Fabbricino, Giovanni Esposito, and Piet N. L. Lens. "In situ and ex situ bioremediation of seleniferous soils from northwestern India." Journal of Soils and Sediments 19, no. 2 (June 23, 2018): 762–73. http://dx.doi.org/10.1007/s11368-018-2055-7.

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8

Hatzinger, Paul B., M. Casey Whittier, Martha D. Arkins, Chris W. Bryan, and William J. Guarini. "In-Situ and Ex-Situ Bioremediation Options for Treating Perchlorate in Groundwater." Remediation Journal 12, no. 2 (March 2002): 69–86. http://dx.doi.org/10.1002/rem.10026.

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9

Yergeau, Etienne, Mélanie Arbour, Roland Brousseau, David Juck, John R. Lawrence, Luke Masson, Lyle G. Whyte, and Charles W. Greer. "Microarray and Real-Time PCR Analyses of the Responses of High-Arctic Soil Bacteria to Hydrocarbon Pollution and Bioremediation Treatments." Applied and Environmental Microbiology 75, no. 19 (August 14, 2009): 6258–67. http://dx.doi.org/10.1128/aem.01029-09.

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ABSTRACT High-Arctic soils have low nutrient availability, low moisture content, and very low temperatures and, as such, they pose a particular problem in terms of hydrocarbon bioremediation. An in-depth knowledge of the microbiology involved in this process is likely to be crucial to understand and optimize the factors most influencing bioremediation. Here, we compared two distinct large-scale field bioremediation experiments, located at the Canadian high-Arctic stations of Alert (ex situ approach) and Eureka (in situ approach). Bacterial community structure and function were assessed using microarrays targeting the 16S rRNA genes of bacteria found in cold environments and hydrocarbon degradation genes as well as quantitative reverse transcriptase PCR targeting key functional genes. The results indicated a large difference between sampling sites in terms of both soil microbiology and decontamination rates. A rapid reorganization of the bacterial community structure and functional potential as well as rapid increases in the expression of alkane monooxygenases and polyaromatic hydrocarbon-ring-hydroxylating dioxygenases were observed 1 month after the bioremediation treatment commenced in the Alert soils. In contrast, no clear changes in community structure were observed in Eureka soils, while key gene expression increased after a relatively long lag period (1 year). Such discrepancies are likely caused by differences in bioremediation treatments (i.e., ex situ versus in situ), weathering of the hydrocarbons, indigenous microbial communities, and environmental factors such as soil humidity and temperature. In addition, this study demonstrates the value of molecular tools for the monitoring of polar bacteria and their associated functions during bioremediation.
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10

Beskoski, Vladimir, Milos Takic, Jelena Milic, Mila Ilic, Gordana Gojgic-Cvijovic, Branimir Jovancicevic, and Miroslav Vrvic. "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on industrial level." Journal of the Serbian Chemical Society 75, no. 11 (2010): 1605–16. http://dx.doi.org/10.2298/jsc100505091b.

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The paper presents results of the ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m3). A treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as the additional carbon source and crude river sand, as bulking and porosity increasing material. The inoculation/reinoculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from the bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders increased 100 times. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg/kg/day for the aliphatic fraction, 6 mg/kg/day for the aromatic fraction, and 3 mg/kg/day for the nitrogen-sulphuroxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyse isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as reduction of relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of hopane type.
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11

Rajamohan, N., R. Manivasagan, and F. Al Fazari. "Treatment of Diesel Oil Contaminated Soil by Ex-situ Bioremediation." Engineering, Technology & Applied Science Research 9, no. 4 (August 10, 2019): 4334–37. http://dx.doi.org/10.48084/etasr.2700.

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Treatment of oil-polluted soil is a challenging problem faced by all refineries and petrochemical industries. In this research study, bioremediation of diesel oil contaminated soil was conducted for diesel concentration ranging from 5% to 20%. The physicochemical characteristics of diesel oil contaminated soil were studied. The effects of soil amendments, namely coconut ash powder, biofilter activated sludge, and NPK fertilizer, on total petroleum hydrocarbon removal efficiency were studied. The maximum total petroleum hydrocarbon removal efficiency achieved was 94.5% when 4g NPK, 40g of activated sludge and 40g of coconut ash powder per 1000g of contaminated soil were used. The studies on the effect of temperature confirmed the optimal temperature as 35°C. The parametric studies confirmed that the degradation efficiency decreased with increase in diesel oil concentration.
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12

Larsen, Sille Bendix, Dimitar Karakashev, Irini Angelidaki, and Jens Ejbye Schmidt. "Ex-situ bioremediation of polycyclic aromatic hydrocarbons in sewage sludge." Journal of Hazardous Materials 164, no. 2-3 (May 30, 2009): 1568–72. http://dx.doi.org/10.1016/j.jhazmat.2008.08.067.

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13

Prpich, George P., Rachel L. Adams, and Andrew J. Daugulis. "Ex situ bioremediation of phenol contaminated soil using polymer beads." Biotechnology Letters 28, no. 24 (September 29, 2006): 2027–31. http://dx.doi.org/10.1007/s10529-006-9189-1.

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14

Horst, John F., Caitlin H. Bell, Andrew Lorenz, Monica Heintz, Yu Miao, Jackie Saling, David Favero, and Shaily Mahendra. "Bioremediation of 1,4‐Dioxane: Successful Demonstration of In Situ and Ex Situ Approaches." Groundwater Monitoring & Remediation 39, no. 4 (October 2019): 15–24. http://dx.doi.org/10.1111/gwmr.12354.

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15

Nayak, Pragati, and Hitesh Solanki. "IMPACT OF AGRICULTURE ON ENVIRONMENT AND BIOREMEDIATION TECHNIQUES FOR IMPROVISATION OF CONTAMINATED SITE." International Association of Biologicals and Computational Digest 1, no. 1 (May 18, 2022): 163–74. http://dx.doi.org/10.56588/iabcd.v1i1.31.

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Sustainable development requires the progress and preferment of environmental management and a persistent exploration and improvement for green technologies to treat a wide range of habitats contaminated by disorganized anthropogenic activities. Bioremediation is an excellent and effective cleaning technique to remove toxic waste from contaminated environment. Bioremediation is vastly involved in degradation, eradication, immobilization, or detoxification of diverse chemical wastes and physical hazardous materials from the surrounding through the all-embracing with the help of microorganisms and plants. Bioremediation technique is widely used to treat wastewater and to remove agricultural chemicals (pesticides and fertilizers) that leach from soil into groundwater. Certain toxic metals, such as mercury, selenium and arsenic compounds, can also be removed from water by bioremediation. Thus, bioremediation is a research and solution oriented technology that needs a prior thorough understanding of different types of available processes for improvisation and clean-up of contaminated sites. In this review, impact agricultural pollution, principle of bioremediation process and, different types of in-situ and ex-situ bioremediation techniques have been discussed.
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16

Nayak, Pragati, and Hitesh Solanki. "IMPACT OF AGRICULTURE ON ENVIRONMENT AND BIOREMEDIATION TECHNIQUES FOR IMPROVISATION OF CONTAMINATED SITE." International Association of Biologicals and Computational Digest 1, no. 1 (May 2, 2022): 145–56. http://dx.doi.org/10.56588/iabcd.v1i1.29.

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Sustainable development requires the progress and preferment of environmental management and a persistent exploration and improvement for green technologies to treat a wide range of habitats contaminated by disorganized anthropogenic activities. Bioremediation is an excellent and effective cleaning technique to remove toxic waste from contaminated environment. Bioremediation is vastly involved in degradation, eradication, immobilization, or detoxification of diverse chemical wastes and physical hazardous materials from the surrounding through the all-embracing with the help of microorganisms and plants. Bioremediation technique is widely used to treat wastewater and to remove agricultural chemicals (pesticides and fertilizers) that leach from soil into groundwater. Certain toxic metals, such as mercury, selenium and arsenic compounds, can also be removed from water by bioremediation. Thus, bioremediation is a research and solution oriented technology that needs a prior thorough understanding of different types of available processes for improvisation and clean-up of contaminated sites. In this review, impact agricultural pollution, principle of bioremediation process and, different types of in-situ and ex-situ bioremediation techniques have been discussed.
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17

Bala, Saroj, Diksha Garg, Banjagere Veerabhadrappa Thirumalesh, Minaxi Sharma, Kandi Sridhar, Baskaran Stephen Inbaraj, and Manikant Tripathi. "Recent Strategies for Bioremediation of Emerging Pollutants: A Review for a Green and Sustainable Environment." Toxics 10, no. 8 (August 19, 2022): 484. http://dx.doi.org/10.3390/toxics10080484.

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Environmental pollution brought on by xenobiotics and other related recalcitrant compounds have recently been identified as a major risk to both human health and the natural environment. Due to their toxicity and non-biodegradability, a wide range of pollutants, such as heavy metals, polychlorinated biphenyls, plastics, and various agrochemicals are present in the environment. Bioremediation is an effective cleaning technique for removing toxic waste from polluted environments that is gaining popularity. Various microorganisms, including aerobes and anaerobes, are used in bioremediation to treat contaminated sites. Microorganisms play a major role in bioremediation, given that it is a process in which hazardous wastes and pollutants are eliminated, degraded, detoxified, and immobilized. Pollutants are degraded and converted to less toxic forms, which is a primary goal of bioremediation. Ex situ or in situ bioremediation can be used, depending on a variety of factors, such as cost, pollutant types, and concentration. As a result, a suitable bioremediation method has been chosen. This review focuses on the most recent developments in bioremediation techniques, how microorganisms break down different pollutants, and what the future holds for bioremediation in order to reduce the amount of pollution in the world.
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18

Zeradjanin, Aleksandra, Jelena Avdalovic, Marija Ljesevic, Olivera Tesic, Srdjan Miletic, Miroslav Vrvic, and Vladimir Beskoski. "Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity." Journal of the Serbian Chemical Society 85, no. 6 (2020): 821–30. http://dx.doi.org/10.2298/jsc190916131z.

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Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation.
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19

Carberry, Judith Bower, and John Wik. "COMPARISON OF EX SITU AND IN SITU BIOREMEDIATION OF UNSATURATED SOILS CONTAMINATED BY PETROLEUM." Journal of Environmental Science and Health, Part A 36, no. 8 (August 31, 2001): 1491–503. http://dx.doi.org/10.1081/ese-100105726.

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20

Dott, Wolfgang, Doris Feidieker, Martin Steiof, Petra M. Becker, and Peter Kämpfer. "Comparison of ex situ and in situ techniques for bioremediation of hydrocarbon-polluted soils." International Biodeterioration & Biodegradation 35, no. 1-3 (January 1995): 301–16. http://dx.doi.org/10.1016/0964-8305(95)00040-c.

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21

Dott, W., D. Feidieker, M. Steiof, P. M. Becker, and P. Kämpfer. "Comparison of ex situ and in situ techniques for bioremediation of hydrocarbon-polluted soils." International Biodeterioration & Biodegradation 35, no. 1-3 (January 1995): 335. http://dx.doi.org/10.1016/0964-8305(95)90040-3.

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22

Yap, How Swen, Nur Nadhirah Zakaria, Azham Zulkharnain, Suriana Sabri, Claudio Gomez-Fuentes, and Siti Aqlima Ahmad. "Bibliometric Analysis of Hydrocarbon Bioremediation in Cold Regions and a Review on Enhanced Soil Bioremediation." Biology 10, no. 5 (April 22, 2021): 354. http://dx.doi.org/10.3390/biology10050354.

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The increased usage of petroleum oils in cold regions has led to widespread oil pollutants in soils. The harsh environmental conditions in cold environments allow the persistence of these oil pollutants in soils for more than 20 years, raising adverse threats to the ecosystem. Microbial bioremediation was proposed and employed as a cost-effective tool to remediate petroleum hydrocarbons present in soils without significantly posing harmful side effects. However, the conventional hydrocarbon bioremediation requires a longer time to achieve the clean-up standard due to various environmental factors in cold regions. Recent biotechnological improvements using biostimulation and/or bioaugmentation strategies are reported and implemented to enhance the hydrocarbon removal efficiency under cold conditions. Thus, this review focuses on the enhanced bioremediation for hydrocarbon-polluted soils in cold regions, highlighting in situ and ex situ approaches and few potential enhancements via the exploitation of molecular and microbial technology in response to the cold condition. The bibliometric analysis of the hydrocarbon bioremediation research in cold regions is also presented.
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23

Ferreira, I. D., and D. M. Morita. "Ex-situ bioremediation of Brazilian soil contaminated with plasticizers process wastes." Brazilian Journal of Chemical Engineering 29, no. 1 (March 2012): 77–86. http://dx.doi.org/10.1590/s0104-66322012000100009.

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24

Paniagua-Michel, J., and O. Garcia. "Ex-situ bioremediation of shrimp culture effluent using constructed microbial mats." Aquacultural Engineering 28, no. 3-4 (August 2003): 131–39. http://dx.doi.org/10.1016/s0144-8609(03)00011-6.

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25

Toffoletto, Laurence, Louise Deschênes, and Réjean Samson. "LCA of Ex-Situ Bioremediation of Diesel-Contaminated Soil (11 pp)." International Journal of Life Cycle Assessment 10, no. 6 (October 18, 2004): 406–16. http://dx.doi.org/10.1065/lca2004.09.180.12.

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26

Sales da Silva, Israel Gonçalves, Fabíola Carolina Gomes de Almeida, Nathália Maria Padilha da Rocha e Silva, Alessandro Alberto Casazza, Attilio Converti, and Leonie Asfora Sarubbo. "Soil Bioremediation: Overview of Technologies and Trends." Energies 13, no. 18 (September 8, 2020): 4664. http://dx.doi.org/10.3390/en13184664.

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Petroleum hydrocarbons, heavy metals and agricultural pesticides have mutagenic, carcinogenic, immunotoxic and teratogenic effects and cause drastic changes in soil physicochemical and microbiological characteristics, thereby representing a serious danger to health and environment. Therefore, soil pollution urgently requires the application of a series of physicochemical and biological techniques and treatments to minimize the extent of damage. Among them, bioremediation has been shown to be an alternative that can offer an economically viable way to restore polluted areas. Due to the difficulty in choosing the best bioremediation technique for each type of pollutant and the paucity of literature on soil bioremediation enhanced by the use of specific additives, we reviewed the main in situ and ex situ methods, their current properties and applications. The first section discusses the characteristics of each class of pollutants in detail, while the second section presents current bioremediation technologies and their main uses, followed by a comparative analysis showing their respective advantages and disadvantages. Finally, we address the application of surfactants and biosurfactants as well as the main trends in the bioremediation of contaminated soils.
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27

Wijayanti, Titik, and Dinna Eka G. Lestari. "BIOREMEDIATION OF CONTAMINATED WASTE BY CADMIUM (Cd) IN WATERS USING INDIGEN BACTERIUM WITH EX-SITU WAY." Jurnal Pena Sains 4, no. 2 (October 29, 2017): 114. http://dx.doi.org/10.21107/jps.v4i2.3207.

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<p><em>The bioremediation technique </em><em>for</em><em> a contaminated liquid waste of heavy metals using indigen</em><em>ous</em> bacteria is a convenient alternative to steps continues to be developed. The research aims to find out the effectiveness of an indigenous bacterial consortium<em></em><em> in bioremediation of contaminated liquid waste </em><em>by</em><em> cadmium </em><em>by</em><em> ex-situ. Experiments </em><em>were</em><em> arranged in RAL made in ex-situ where a liquid waste industry was given five treatments, namely control and four indigen</em><em>ous</em><em> bacterial consortia (A, D, E, and J) obtained from the isolation of bacteria originating from cadmium-contaminated of waste in Pasuruan </em><em>district</em><em>. Furthermore conducted observations of BOD<sub>5</sub>, COD, d.o. and Cd for seven days to find out the effectiveness of bioremediation. The results showed the four </em><em>indigenous </em><em>bacteria consortia have the bioremediation ability to reduce levels of </em><em>cadmium, </em><em>BOD<sub>5</sub>, COD, and increasing levels of DO. Indigen</em><em>ous</em><em> bacterial consortia D </em><em>has</em><em> the </em><em>best </em><em>ability of liquid industrial waste bioremediation </em><em>by</em><em> ex-situ. Indigen</em><em>ous</em><em> bacteria</em><em>l</em><em> consortia J </em><em>has</em><em> the </em><em>best of </em><em>capacity reduction levels of cadmium, </em><em>then the other of </em><em>indigen</em><em>ous</em><em> bacteria</em><em>l </em><em>consortia.</em><em></em></p><strong><em>Keywords:</em><em> indigenous bacterial, bioremediation, ex-situ, cadmium, liquid waste.</em></strong>
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Mariano, Adriano Pinto, Sérgio Henrique Rezende Crivelaro, Dejanira de Franceschi de Angelis, and Daniel Marcos Bonotto. "The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil." Brazilian Archives of Biology and Technology 52, no. 4 (August 2009): 1043–55. http://dx.doi.org/10.1590/s1516-89132009000400030.

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This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.
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Mohajeri, Leila, Hamidi Abdul Aziz, Mohamed Hasnain Isa, Mohammad Ali Zahed, and Soraya Mohajeri. "Ex-situ Bioremediation of Crude Oil in Soil, a Comparative Kinetic Analysis." Bulletin of Environmental Contamination and Toxicology 85, no. 1 (June 25, 2010): 54–58. http://dx.doi.org/10.1007/s00128-010-0058-1.

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30

Höckenreiner, M., H. Neugebauer, and L. Elango. "Ex situ bioremediation method for the treatment of groundwater contaminated with PAHs." International Journal of Environmental Science and Technology 12, no. 1 (December 5, 2013): 285–96. http://dx.doi.org/10.1007/s13762-013-0427-5.

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31

Jabbar, Noor Mohsen, Estabriq Hasan Kadhim, and Alaa Kareem Mohammed. "Bioremediation of Soil Contaminated with Diesel using Biopile system." Al-Khwarizmi Engineering Journal 14, no. 3 (August 15, 2018): 48–56. http://dx.doi.org/10.22153/https://doi.org/10.22153/kej.2018.12.009.

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This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition). Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.
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Jabbar, Noor Mohsen, Estabriq Hasan Kadhim, and Alaa Kareem Mohammed. "Bioremediation of Soil Contaminated with Diesel using Biopile system." Al-Khwarizmi Engineering Journal 14, no. 3 (August 15, 2018): 48–56. http://dx.doi.org/10.22153/kej.2018.12.009.

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This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition). Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.
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33

Alam, Sk Aftabul, and Pradipta Saha. "Microbial biodegradation of nitrophenols and their derivatives: A Review." Journal of Experimental Biology and Agricultural Sciences 10, no. 4 (August 30, 2022): 743–66. http://dx.doi.org/10.18006/2022.10(4).743.766.

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Today, nitrophenols (NPs) represent chemicals highly in demand not only due to their function in synthetic chemistry but also due to their huge applications in several industries. Such diverse requirements and applications has resulted in a widespread abundance of these chemicals. Improper application and waste disposal practice results in the continuous discharge of these compounds into the environment and causes pollution threat to soil, groundwater, river water, etc. These xenobiotic chemicals are hazardous, toxic, carcinogenic, and mutagenic which results in serious health problems. The Nitro group present in the phenol makes them recalcitrant which causes the persistence of these chemicals in the environment. Although several chemicals, electrochemical, physical, and physicochemical methods have been proposed, bioremediation approaches mainly involving bacteria are considered best. To date, very few successful attempts (related to microbe-assisted bioremediation) have been carried out with environmental habitats for the removal of NPs (both in-situ and ex-situ attempts). So, as far as the effectiveness of the bioremediation process for NP decontamination is concerned, we are far away. More explorative studies using efficient aerobic-anaerobic NP degrading bacterial consortium (or combination of microbes- plant systems) and advanced techniques including omics approaches and nanotechnologies may help towards developing better practicable bioremediation approaches, in the future. This review article focuses on the list of nitrophenol degrading microorganisms, biodegradation pathways of NPs, bioremediation by immobilized cell technique, and the advantages and disadvantages of bioremediation. This article will increase our knowledge of the biodegradation of NPs.
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Micle, Valer, Ioana Monica Sur, Adriana Criste, Marin Senila, Erika Levei, Mariana Marinescu, Carmen Cristorean, and George Calin Rogozan. "Lab-scale experimental investigation concerning ex-situ bioremediation of petroleum hydrocarbons-contaminated soils." Soil and Sediment Contamination: An International Journal 27, no. 8 (August 10, 2018): 692–705. http://dx.doi.org/10.1080/15320383.2018.1503229.

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35

Tomei, M. Concetta, and Andrew J. Daugulis. "Ex Situ Bioremediation of Contaminated Soils: An Overview of Conventional and Innovative Technologies." Critical Reviews in Environmental Science and Technology 43, no. 20 (November 5, 2012): 2107–39. http://dx.doi.org/10.1080/10643389.2012.672056.

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36

Gomez, Francisco, and Majid Sartaj. "Field scale ex-situ bioremediation of petroleum contaminated soil under cold climate conditions." International Biodeterioration & Biodegradation 85 (November 2013): 375–82. http://dx.doi.org/10.1016/j.ibiod.2013.08.003.

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37

Demeter, Marc A., Joe Lemire, Iain George, Gordon Yue, Howard Ceri, and Raymond J. Turner. "Harnessing oil sands microbial communities for use in ex situ naphthenic acid bioremediation." Chemosphere 97 (February 2014): 78–85. http://dx.doi.org/10.1016/j.chemosphere.2013.11.016.

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38

Priyanka, Anil Kumar, Vinod Chhokar, and Vikas Beniwal. "UNDERSTANDING THE ROLE OF BACTERIAL GENES AND ENZYMES IN ORGANOPHOSPHATE DEGRADATION: A STEP TOWARDS ENHANCED BIOREMEDIATION." International Journal of Biological Innovations 05, no. 01 (2023): 143–54. http://dx.doi.org/10.46505/ijbi.2023.5112.

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The use of Organophosphates (OPs) as pesticides and insecticides increased after world war II, although organophosphates (OPs) are comparatively less persistent in the environment, but are highly toxic to animals. OP toxicity is causing a threat to biodiversity, hence it becomes essential to deal with the degradation of such compounds. Various techniques like photolysis, and chemical degradation have been used for OP degradation but these techniques are not costeffective and require ex-situ treatment, hence bioremediation is considered a potential alternative for OP degradation. Understanding the degradation pathways followed by different bacteria, genes and enzymes involved in such pathways can act as a step towards the development of an effective bioremediation technique for OP degradation. Recombinant biotechnology and protein engineering are used to develop designer bacteria, biocatalysts and enzymes with enhanced activity for OP-degrading bacteria. The present review highlights the bacterial degradation pathways, genes and enzymes involved in bioremediation pathways and new approaches for the development of OP bioremediation techniques.
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39

Singh, Shreya, and Ningombam Linthoingambi Devi. "BIOREMEDIATION OF PAHS CONTAMINATED AGRICULTURAL SOIL-A REVIEW PAPER." International Journal on Environmental Sciences 13, no. 02 (2022): 66–72. http://dx.doi.org/10.53390/ijes.v13i2.2.

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Soil is an important environmental matrix that directly or indirectly supports the life of all creatures. Despite being the ultimate sink for all contaminants, it has been neglected for a long time, resulting in poor soil quality. Due to the contamination of various toxic polycyclic aromatic compounds (PAHs) in soil, it diverts the quality of soil and impacts the soil ecosystem. Henceforth, it is necessary to identify the ecologically sustainable treatment alternatives for contaminated site cleanup. Biological treatment of PAHs contaminated soil is emerging as a promising and sustainable treatment options because they are safe, cost effective and eco-friendly treatment solutions. When it comes to pollutant degradation, microorganisms are known for their enzyme-catalyzed catabolic activity, w hich can be advantageous in the decomposition of PAHs. There are various microbes which are extensively used for the removal of PAHs, in which This review paper compiled a various recent in-situ and ex-situ bioremediation techniques used for the degradation and remediation of PAHs in agricultural soil.
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Perini, Brayam Luiz Batista, Naionara Ariete Daronch, Rodrigo Luiz Bitencourt, Andréa Lima dos Santos Schneider, Cristiano José de Andrade, and Débora de Oliveira. "Application of Immobilized Laccase on Polyurethane Foam for Ex-Situ Polycyclic Aromatic Hydrocarbons Bioremediation." Journal of Polymers and the Environment 29, no. 7 (January 12, 2021): 2200–2213. http://dx.doi.org/10.1007/s10924-020-02035-9.

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41

Kamarudheen, Neethu, Sona P. Chacko, Catherin A. George, Rakhi Chettiparambil Somachandran, and K. V. Bhaskara Rao. "An ex-situ and in vitro approach towards the bioremediation of carcinogenic hexavalent chromium." Preparative Biochemistry & Biotechnology 50, no. 8 (April 17, 2020): 842–48. http://dx.doi.org/10.1080/10826068.2020.1755868.

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42

Firmino, Paulo Igor M., Raquel S. Farias, Amanda N. Barros, Patrícia M. C. Buarque, Elisa Rodríguez, Alexandre C. Lopes, and André B. dos Santos. "Understanding the anaerobic BTEX removal in continuous-flow bioreactors for ex situ bioremediation purposes." Chemical Engineering Journal 281 (December 2015): 272–80. http://dx.doi.org/10.1016/j.cej.2015.06.106.

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43

Rojas-Avelizapa, N. G., T. Roldán-Carrillo, H. Zegarra-Martínez, A. M. Muñoz-Colunga, and L. C. Fernández-Linares. "A field trial for an ex-situ bioremediation of a drilling mud-polluted site." Chemosphere 66, no. 9 (January 2007): 1595–600. http://dx.doi.org/10.1016/j.chemosphere.2006.08.011.

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44

Lin, Ta-Chen, Po-Tsen Pan, Chiu-Chung Young, Jo-Shu Chang, Tsung-Chung Chang, and Sheng-Shung Cheng. "Evaluation of the optimal strategy for ex situ bioremediation of diesel oil-contaminated soil." Environmental Science and Pollution Research 18, no. 9 (May 3, 2011): 1487–96. http://dx.doi.org/10.1007/s11356-011-0485-5.

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45

Abbassi, Bassim E., and Walid D. Shquirat. "Kinetics of Indigenous Isolated Bacteria used for Ex-Situ Bioremediation of Petroleum Contaminated Soil." Water, Air, and Soil Pollution 192, no. 1-4 (April 4, 2008): 221–26. http://dx.doi.org/10.1007/s11270-008-9649-4.

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46

Kalogerakis, Nicolas. "ChemInform Abstract: Ex situ Bioremediation of Contaminated Soils: From Biopiles to Slurry-Phase Bioreactors." ChemInform 43, no. 41 (September 13, 2012): no. http://dx.doi.org/10.1002/chin.201241276.

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47

Sayara, Tahseen, and Antoni Sánchez. "Bioremediation of PAH-Contaminated Soils: Process Enhancement through Composting/Compost." Applied Sciences 10, no. 11 (May 26, 2020): 3684. http://dx.doi.org/10.3390/app10113684.

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Bioremediation of contaminated soils has gained increasing interest in recent years as a low-cost and environmentally friendly technology to clean soils polluted with anthropogenic contaminants. However, some organic pollutants in soil have a low biodegradability or are not bioavailable, which hampers the use of bioremediation for their removal. This is the case of polycyclic aromatic hydrocarbons (PAHs), which normally are stable and hydrophobic chemical structures. In this review, several approaches for the decontamination of PAH-polluted soil are presented and discussed in detail. The use of compost as biostimulation- and bioaugmentation-coupled technologies are described in detail, and some parameters, such as the stability of compost, deserve special attention to obtain better results. Composting as an ex situ technology, with the use of some specific products like surfactants, is also discussed. In summary, the use of compost and composting are promising technologies (in all the approaches presented) for the bioremediation of PAH-contaminated soils.
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48

Hu, Guiping. "Isolation of an Indigenous Imidacloprid-Degrading Bacterium and Imidacloprid Bioremediation Under Simulated In Situ and Ex Situ Conditions." Journal of Microbiology and Biotechnology 23, no. 11 (November 2013): 1617–26. http://dx.doi.org/10.4014/jmb.1305.05048.

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49

Abejero, Alma Lorelei, Antonio Alcantara, Lorele Trinidad, and Maxima Flavier. "Kapok (Ceiba pentandra L. Gaertn.) Fibers Packed in Nylon Nets as Sorbent for Diesel Oil Spill and its ex-situ Bioremediation." Journal of Environmental Science and Management 16, no. 1 (June 30, 2013): 72–83. http://dx.doi.org/10.47125/jesam/2013_1/09.

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The effects of oil spill on the ecosystem and human lives are unprecedented. Early response and containment of the oil spill is the best approach in reducing the environmental impacts. This study assessed the diesel oil absorption capacity of Kapok fiber packed in Nylon net then tested the ability of a consortium of bacterial species reported to have oil degrading properties. To evaluate the conditions for the application of ex situ bioremediation, the hydrocarbon conversion was determined qualitatively by monitoring some possible degradation products with hexadecane as reference. Kapok (Ceiba pentandra (L.) Gaertn.) fibers packed in Nylon net were found effective in adsorbing diesel oil with a sorption capacity of 15.5 g g-1 fibers. A consortium of Bacillus megaterium, Corynebacterium flavescens, Micrococcus luteus and Pseudomonas putida with nutrient amendment (0.15 g N and 0.03 g P gram-1 oil) was used to determine preliminary oil biodegradation. Microbial population was sustained for six weeks and all species were found to contribute in the degradation process. Biosurfactant production was also observed in the seawater media. Gas chromatographic analysis showed some degradation products of the adsorbed diesel oil after one week of treatment. The use of Kapok sorbents for Tier 1 and 2 oil spill clean-up and its bioremediation done ex situ to degrade the diesel oil hydrocarbons can be an option.
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Juneau, Armand A., Ellen Moyer, and Joseph E. O'Connell. "Case study ofex situ remediation and conversion to a combinedin situ/ex situ bioremediation approach at an oxygenated gasoline release site." Remediation Journal 17, no. 2 (2007): 19–37. http://dx.doi.org/10.1002/rem.20122.

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