Log in

Relevant bibliographies by topics / Ex vivo expanded cartilage cells / Journal articles

To see the other types of publications on this topic, follow the link: Ex vivo expanded cartilage cells.

Journal articles on the topic 'Ex vivo expanded cartilage cells'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Ex vivo expanded cartilage cells.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Goh, Doreen, Yanmeng Yang, Eng Hin Lee, James Hoi Po Hui, and Zheng Yang. "Managing the Heterogeneity of Mesenchymal Stem Cells for Cartilage Regenerative Therapy: A Review." Bioengineering 10, no. 3 (2023): 355. http://dx.doi.org/10.3390/bioengineering10030355.

Full text

Abstract:

Articular cartilage defects commonly result from trauma and are associated with significant morbidity. Since cartilage is an avascular, aneural, and alymphatic tissue with a poor intrinsic healing ability, the regeneration of functional hyaline cartilage remains a difficult clinical problem. Mesenchymal stem cells (MSCs) are multipotent cells with multilineage differentiation potential, including the ability to differentiate into chondrocytes. Due to their availability and ease of ex vivo expansion, clinicians are increasingly applying MSCs in the treatment of cartilage lesions. However, despi

APA, Harvard, Vancouver, ISO, and other styles

2

Jakob, Yvonne, Johann Kern, David Gvaramia, et al. "Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction." Cells 13, no. 2 (2024): 141. http://dx.doi.org/10.3390/cells13020141.

Full text

Abstract:

Tissue engineering (TE) techniques offer solutions for tissue regeneration but require large quantities of cells. For microtia patients, TE methods represent a unique opportunity for therapies with low donor-site morbidity and reliance on the surgeon’s individual expertise. Microtia-derived chondrocytes and perichondrocytes are considered a valuable cell source for autologous reconstruction of the pinna. The aim of this study was to investigate the suitability of perichondrocytes from microtia patients for autologous reconstruction in comparison to healthy perichondrocytes and microtia chondro

APA, Harvard, Vancouver, ISO, and other styles

3

Trubiani, O., R. Di Primio, T. Traini, et al. "Morphological and Cytofluorimetric Analysis of Adult Mesenchymal Stem Cells Expanded Ex Vivo from Periodontal Ligament." International Journal of Immunopathology and Pharmacology 18, no. 2 (2005): 213–21. http://dx.doi.org/10.1177/039463200501800204.

Full text

Abstract:

Many adult tissues contain a population of stem cells that have the ability of regeneration after trauma, disease or aging. Recently, there has been great interest in mesenchymal stem cells and their roles in maintaining physiological structure tissues and their studies have been considered very important and intriguing after having shown that this cell population can be expanded ex vivo to regenerate tissues not only of the mesenchymal lineage, such as intervertebral disc cartilage, bone, tooth-associated tissue, cardiomyocytes, but also to differentiate into cells derived from other embryoni

APA, Harvard, Vancouver, ISO, and other styles

4

Krueger, Simone, Sophie Achilles, Julius Zimmermann, Thomas Tischer, Rainer Bader, and Anika Jonitz-Heincke. "Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields." Journal of Clinical Medicine 8, no. 11 (2019): 1771. http://dx.doi.org/10.3390/jcm8111771.

Full text

Abstract:

Treatment of cartilage lesions remains a clinical challenge. Therefore, biophysical stimuli like electric fields seem to be a promising tool for chondrocytic differentiation and treatment of cartilage lesions. In this in vitro study, we evaluated the effects of low intensity capacitively coupled electric fields with an alternating voltage of 100 mVRMS (corresponds to 5.2 × 10−5 mV/cm) or 1 VRMS (corresponds to 5.2 × 10−4 mV/cm) with 1 kHz, on human chondrocytes derived from osteoarthritic (OA) and non-degenerative hyaline cartilage. A reduction of metabolic activity after electrical stimulatio

APA, Harvard, Vancouver, ISO, and other styles

5

Pei, Yixuan Amy, Jhanvee Patel, and Ming Pei. "Extracellular Matrix Tunes the Regenerative Potential of Fetal Stem Cells." Applied Sciences 14, no. 5 (2024): 1932. http://dx.doi.org/10.3390/app14051932.

Full text

Abstract:

Adult mesenchymal stem cells (MSCs) are a promising cell source for tissue regeneration. However, ex vivo expansion results in cell senescence; cells lose their proliferation and differentiation capacity. Fetal MSCs can offer an alternative due to their robust proliferation and differentiation capacities, as well as their immune privilege properties. Given the rejuvenation effect of the decellularized extracellular matrix (dECM) on adult MSCs, it remains unknown whether dECM influences the regenerative potential of fetal stem cells. In this study, passage five fetal nucleus pulposus cells (fNP

APA, Harvard, Vancouver, ISO, and other styles

6

Busse, Dan. "Increased Knee Cartilage Volume in Degenerative Joint Disease using Percutaneously Implanted, Autologous Mesenchymal Stem Cells." Pain Physician 3;11, no. 5;3 (2008): 343–53. http://dx.doi.org/10.36076/ppj.2008/11/343.

Full text

Abstract:

Background: The ability to repair tissue via percutaneous means may allow interventional pain physicians to manage a wide variety of diseases including peripheral joint injuries and osteoarthritis. This review will highlight the developments in cellular medicine that may soon permit interventional pain management physicians to treat a much wider variety of clinical conditions and highlight an interventional case study using these technologies Objective: To determine if isolated and expanded human autologous mesenchymal stem cells could effectively regenerate cartilage and meniscal tissue when

APA, Harvard, Vancouver, ISO, and other styles

7

Li, Siming, Xiaohong Yang, Zhencheng Feng, Pengzhen Wang, Weicong Zhu та Shuliang Cui. "Catalase Enhances Viability of Human Chondrocytes in Culture by Reducing Reactive Oxygen Species and Counteracting Tumor Necrosis Factor-α-Induced Apoptosis". Cellular Physiology and Biochemistry 49, № 6 (2018): 2427–42. http://dx.doi.org/10.1159/000493841.

Full text

Abstract:

Background/Aims: Both physiologic remodeling and pathologic regeneration of cartilage tissue rely upon chondrocyte functions and are benefited from factors that promote viability and inhibit apoptosis of the cell, and associated mechanisms. High level of reactive oxygen species (ROS) and proinflammatory cytokines activate apoptosis signaling and initiate cell death, which can be attenuated by antioxidants. This study examined the effect of catalase (CAT) on ROS and tumor necrosis factor-α (TNF-α)-induced apoptosis in human C28/I2 chondrocytes cultured in monolayer. Methods: Chondrocytes were t

APA, Harvard, Vancouver, ISO, and other styles

8

Kim, Dong Hwan, Bo Young Kim, Dong Hyun Kim, Jin Hur, and Chung-Hwan Baek. "Rabbit palatum-derived mesenchymal progenitor cells tri-lineage differentiation on 2D substrates and 3D printed constructs." Journal of Applied Biomaterials & Functional Materials 17, no. 3 (2019): 228080001983452. http://dx.doi.org/10.1177/2280800019834520.

Full text

Abstract:

Hard palate, developed by embryo neural crest stem cells, is a tissue with strong regenerative abilities. It is considered an abundant source of progenitor cells, forming various mesenchymal tissues. Rabbits are more suitable models than murine animals for regenerative preclinical study of the head and neck, owing to their larger size. However, there are no reports of the existence or characteristics of neural crest stem cells in the hard palate of rabbits. In this study, we demonstrate for the first time the presence of nestin-, Sox2-, and p75-positive neural crest stem cells obtained from th

APA, Harvard, Vancouver, ISO, and other styles

9

Jamnig, Angelika, and Günter Lepperdinger. "From tendon to nerve: an MSC for all seasons." Canadian Journal of Physiology and Pharmacology 90, no. 3 (2012): 295–306. http://dx.doi.org/10.1139/y11-109.

Full text

Abstract:

The potential of mesenchymal stem cells (MSCs) to regenerate damaged tissue is well documented, as this specialized progenitor cell type exhibits superior cellular properties, and would allow medical as well as ethical limitations to be overcome. By now, MSCs have been successfully introduced in manifold experimental approaches within the newly defined realm of Regenerative Medicine. Advanced methods for in vitro cell expansion, defined induction of distinct differentiation processes, 3-dimensional culture on specific scaffold material, and tissue engineering approaches have been designed, and

APA, Harvard, Vancouver, ISO, and other styles

10

Taketani, Takeshi, Rie Kanai, Aya Mihara, et al. "Transplantation of Ex Vivo Expanded Allogeneic Mesenchymal Stem Cells with Bone Marrow Improved Osteogenesis and Bone Formation In Patients with Perinatal Hypophosphatasia." Blood 116, no. 21 (2010): 4690. http://dx.doi.org/10.1182/blood.v116.21.4690.4690.

Full text

Abstract:

Abstract Abstract 4690 Hypophosphatasia (HPP) is one of the bone metabolic disorders caused by mutations of the liver/bone/kidney alkaline phosphatase (ALPL) gene encoding tissue-nonspecific alkaline phosphatase (ALP). The disease is characterized by the disturbance of bone and tooth mineralization and reduced serum ALP activity. Clinical severity of HPP often depends on the age of onset. Patients with perinatal and infantile forms usually have poor prognosis. Phenotype in patients with HPP is also closely related to residual enzyme activity affected by ALPL mutations. Most perinatal patients

APA, Harvard, Vancouver, ISO, and other styles

11

Fan, Huahua, Xiaona Huo, Juan Sun, Yiming Yang, and Xiao Li. "Efficient Induction and Expansion Of CD8+CD28+Foxp3+ Regulatory T Cells By TGF-beta1 and Rapamycin." Blood 122, no. 21 (2013): 190. http://dx.doi.org/10.1182/blood.v122.21.190.190.

Full text

Abstract:

Abstract Background Although extensive studies have focused on the CD4+CD25+Foxp3+Tregs in recent years, CD8+Tregs have also been reported to play important roles in maintenance of immune tolerance. Adoptive transfer of CD8+Tregs in rodents can prevent or treat autoimmune diseases, allograft rejection or graft-versus-host disease. Objective Several approaches for induction of Ag-specific CD8+Tregs have been reported, but there is currently no reliable protocol for the ex vivo induction and large-scale expansion of human polyclonal CD8+CD28+Foxp3+Tregs. Our research was designed to investigate

APA, Harvard, Vancouver, ISO, and other styles

12

Altaie, A., E. Jones, O. Wall, and D. Mcgonagle. "FRI0389 TOWARDS ONE-STAGE ARTICULAR CARTILAGE REPAIR USING DEVICE BASED ARTHROSCOPIC MECHANICAL RELEASE OF SYNOVIAL MESENCHYMAL STEM CELLS WITH CHONDROGENESIS AUGMENTATION WITH AUTOLOGOUS PLATELET CONCENTRATE WITHOUT CELL CULTURE EXPANSION." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 792–93. http://dx.doi.org/10.1136/annrheumdis-2020-eular.5717.

Full text

Abstract:

Background:Synovial fluid contains resident mesenchymal stem cells (SF-MSCs) that are derived from the synovial membrane and may interact with superficial cartilage injury sites. We previously reported on a novel methodology for increasing the number of MSCs in the knee joints using synovial brushing combined with platelet lysate (PL) as a chondrogenic inducer [1, 2].Objectives:The purpose of this study was to evaluate autologous and allogenic PL as a chondrogenic inducer and the chondrogenic potential of the mobilised MSCs without further ex vivo expansion. The desired goal of the study was t

APA, Harvard, Vancouver, ISO, and other styles

13

Debeljak-Martacic, Jasmina, Jelena Francuski, Tijana Luzajic, et al. "Characterization of deciduous teeth stem cells isolated from crown dental pulp." Vojnosanitetski pregled 71, no. 8 (2014): 735–41. http://dx.doi.org/10.2298/vsp1408735d.

Full text

Abstract:

Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expa

APA, Harvard, Vancouver, ISO, and other styles

14

Soler, Robert, Lluis Orozco, Ana Munar, et al. "Final results of a phase I–II trial using ex vivo expanded autologous Mesenchymal Stromal Cells for the treatment of osteoarthritis of the knee confirming safety and suggesting cartilage regeneration." Knee 23, no. 4 (2016): 647–54. http://dx.doi.org/10.1016/j.knee.2015.08.013.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Renda, Maria Concetta, Emanuela Fecarotta, Giovanna Schillaci, et al. "Mesenchymal Fetal Stem Cells (FMSC) from Amniotic Fluid (AF): Expansion and Phenotypic Characterization." Blood 126, no. 23 (2015): 4758. http://dx.doi.org/10.1182/blood.v126.23.4758.4758.

Full text

Abstract:

Abstract Introduction. Mesenchymal stem cells (MSCs) are multipotent precursors able to differentiate into bone, cartilage and fat. In humans, the MCS were isolated from bone marrow, cord blood, peripheral blood, adipose tissue and the amniotic fluid (AF) in the second trimester of pregnancy (FA-MSC). The FA-MCS have immunosuppressive properties, extensive proliferative potential and self-renewal, and express the fetal stem transcription factor Oct4. The purpose of this work was to isolate and characterize cells isolated from human FA as alternative source of multipotent MSC for regenerative m

APA, Harvard, Vancouver, ISO, and other styles

16

Kim, Jaehyup, and Peiman Hematti. "Mesenchymal Stem Cells Convert Human Macrophages to a Novel Type of Alternatively Activated Macrophages." Blood 114, no. 22 (2009): 3632. http://dx.doi.org/10.1182/blood.v114.22.3632.3632.

Full text

Abstract:

Abstract Abstract 3632 Poster Board III-568 Mesenchymal stem cells (MSCs) are capable of modulating the immune system through interaction with a wide range of immune cells. This study investigates the hypothesis that interaction of MSCs with macrophages could play a significant role in their anti-inflammatory/immune modulatory effects. All studies were approved by IRB of University of Wisconsin School of Medicine and Public Health. MSCs were culture expanded from discarded bone marrow filters after bone marrow harvest from normal healthy sibling HLA-matched donors. We used passages -35 for our

APA, Harvard, Vancouver, ISO, and other styles

17

Homicz, Mark R., Barbara L. Schumacher, Robert L. Sah, and Deborah Watson. "Effects of Serial Expansion of Septal Chondrocytes on Tissue-Engineered Neocartilage Composition." Otolaryngology–Head and Neck Surgery 127, no. 5 (2002): 398–408. http://dx.doi.org/10.1067/mhn.2002.129730.

Full text

Abstract:

OBJECTIVES: Cartilage grafts for reconstructive surgery may someday be created from harvested autologous chondrocytes that are expanded and seeded onto biodegradable scaffolds in vitro. This study sought to quantify the biochemical composition of neocartilage engineered from human septal chondrocytes and to examine the effects of cell multiplication in monolayer culture on the ultimate composition of the neocartilage. METHODS: Human septal chondrocytes from 10 donors were either seeded immediately after harvest (passage 0 (P0)) onto polyglycolic acid (PGA) scaffolds or underwent multiplication

APA, Harvard, Vancouver, ISO, and other styles

18

Mengis, Tamara, Laura Bernhard, Andrea Nüesch, et al. "The Expression of Toll-like Receptors in Cartilage Endplate Cells: A Role of Toll-like Receptor 2 in Pro-Inflammatory and Pro-Catabolic Gene Expression." Cells 13, no. 17 (2024): 1402. http://dx.doi.org/10.3390/cells13171402.

Full text

Abstract:

Introduction: The vertebral cartilage endplate (CEP), crucial for intervertebral disc health, is prone to degeneration linked to chronic low back pain, disc degeneration, and Modic changes (MC). While it is known that disc cells express toll-like receptors (TLRs) that recognize pathogen- and damage-associated molecular patterns (PAMPs and DAMPs), it is unclear if CEP cells (CEPCs) share this trait. The CEP has a higher cell density than the disc, making CEPCs an important contributor. This study aimed to identify TLRs on CEPCs and their role in pro-inflammatory and catabolic gene expression. M

APA, Harvard, Vancouver, ISO, and other styles

19

Tschiedel, Sabine, Kristina Bartsch, Annette Reinhardt, Gentilini Chiara, and Niederwieser Dietger. "Mesenchymal Stem Cells Induce a Reversible Suppression of Alloimmune Reactions." Blood 106, no. 11 (2005): 4316. http://dx.doi.org/10.1182/blood.v106.11.4316.4316.

Full text

Abstract:

Abstract Objective Bone marrow contains pluripotent mesenchymal stem cells (MSC) that form bone, cartilage, adipose tissue and muscle. These cells are not immunogenic and escape recognition by alloreactive T cells and natural killer cells. MSC can be readily isolated from bone marrow and expanded ex vivo without modification in phenotype or loss of function. They are capable of differentiating along multiple mesenchymal lineages, play a crucial role in the bone marrow microenvironment and have profound immunosuppressive properties. In the present investigation we analysed the antiproliferative

APA, Harvard, Vancouver, ISO, and other styles

20

Jaroscak, Jennifer, Kristin Goltry, Alan Smith, et al. "Augmentation of umbilical cord blood (UCB) transplantation with ex vivo–expanded UCB cells: results of a phase 1 trial using the AastromReplicell System." Blood 101, no. 12 (2003): 5061–67. http://dx.doi.org/10.1182/blood-2001-12-0290.

Full text

Abstract:

AbstractAllogeneic stem cell transplantation with umbilical cord blood (UCB) cells is limited by the cell dose a single unit provides recipients. Ex vivo expansion is one strategy to increase the number of cells available for transplantation. Aastrom Biosciences developed an automated continuous perfusion culture device for expansion of hematopoietic stem cells (HSCs). Cells are expanded in media supplemented with fetal bovine serum, horse serum, PIXY321, flt-3 ligand, and erythropoietin. We performed a phase 1 trial augmenting conventional UCB transplants with ex vivo–expanded cells. The 28 p

APA, Harvard, Vancouver, ISO, and other styles

21

Kim, Jung-Sik, Kyeo-Rae Han, and Chung-Gyu Park. "Novel Consistently Ex-vivo Expanded CD8+CD25+FoxP3+Treg Cells Prolongs the Allogeneic Islet Survival." Journal of Immunology 200, no. 1_Supplement (2018): 55.39. http://dx.doi.org/10.4049/jimmunol.200.supp.55.39.

Full text

Abstract:

Abstract In addition to CD4+ regulatory T cells(Tregs), adoptive transfer of CD8+CD25+FoxP3+T cells is emerging as an alternative adjunctive therapy to diminish current reliance on lifelong, nonspecific immunosuppression after transplantation. Here we evaluated the possible therapeutic application of consistently ex-vivo expanded mouse CD8+CD25+FoxP3+T cells to prevent the rejection of allogeneic islets. Around 80% of mouse CD8+CD25+T and CD4+CD25+T cells expressed FoxP3+T cells in spleen. Higher level of FoxP3 was expressed in CD8+Tregs compared to CD4+Tregs. Ex-vivo enriched CD8+CD25+Treg ce

APA, Harvard, Vancouver, ISO, and other styles

22

Deng, Xuewen, Hiroshi Terunuma, Atsushi Terunuma, Tsubasa Takane та Mie Nieda. "Ex vivo-expanded natural killer cells kill cancer cells more effectively than ex vivo-expanded γδ T cells or αβ T cells". International Immunopharmacology 22, № 2 (2014): 486–91. http://dx.doi.org/10.1016/j.intimp.2014.07.036.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Güenechea, G., J. C. Segovia, B. Albella, et al. "Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo–Expanded Human CD34+ Cord Blood Cells." Blood 93, no. 3 (1999): 1097–105. http://dx.doi.org/10.1182/blood.v93.3.1097.

Full text

Abstract:

Abstract The ex vivo expansion of hematopoietic progenitors is a promising approach for accelerating the engraftment of recipients, particularly when cord blood (CB) is used as a source of hematopoietic graft. With the aim of defining the in vivo repopulating properties of ex vivo–expanded CB cells, purified CD34+ cells were subjected to ex vivo expansion, and equivalent proportions of fresh and ex vivo–expanded samples were transplanted into irradiated nonobese diabetic (NOD)/severe combined immunodeficient (SCID) mice. At periodic intervals after transplantation, femoral bone marrow (BM) sam

APA, Harvard, Vancouver, ISO, and other styles

24

Güenechea, G., J. C. Segovia, B. Albella, et al. "Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo–Expanded Human CD34+ Cord Blood Cells." Blood 93, no. 3 (1999): 1097–105. http://dx.doi.org/10.1182/blood.v93.3.1097.403k04_1097_1105.

Full text

Abstract:

The ex vivo expansion of hematopoietic progenitors is a promising approach for accelerating the engraftment of recipients, particularly when cord blood (CB) is used as a source of hematopoietic graft. With the aim of defining the in vivo repopulating properties of ex vivo–expanded CB cells, purified CD34+ cells were subjected to ex vivo expansion, and equivalent proportions of fresh and ex vivo–expanded samples were transplanted into irradiated nonobese diabetic (NOD)/severe combined immunodeficient (SCID) mice. At periodic intervals after transplantation, femoral bone marrow (BM) samples were

APA, Harvard, Vancouver, ISO, and other styles

25

Hristova, R., Y. Zdravkov, and I. Tanev. "Transplantation of ex vivo expanded limbal stem cells." Bulgarian Review of Ophthalmology 61, no. 2 (2017): 32. http://dx.doi.org/10.14748/bro.v0i2.4513.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Li, Tao-Sheng, Masanori Hayashi, Ze-Lin Liu, et al. "Low angiogenic potency induced by the implantation of ex vivo expanded CD117+stem cells." American Journal of Physiology-Heart and Circulatory Physiology 286, no. 4 (2004): H1236—H1241. http://dx.doi.org/10.1152/ajpheart.00950.2003.

Full text

Abstract:

Ex vivo expansion of stem cells might be a feasible method of resolving the problem of limited cell supply in cell-based therapy. The implantation of expanded CD34+endothelial progenitor cells has the capacity to induce angiogenesis. In this study, we tried to induce angiogenesis by implanting expanded CD117+stem cells derived from mouse bone marrow. After 2 wk of culture with the addition of several growth factors, the CD117+stem cells expanded ∼20-fold and had an endothelial phenotype with high expression of CD34 and vascular endothelial-cadherin. However, >70% of these ex vivo expanded c

APA, Harvard, Vancouver, ISO, and other styles

27

Sega, Emanuela I., Dennis Leveson-Gower, Vu H. Nguyen, and Robert Negrin. "Functional Comparison of Freshly Isolated and Ex-Vivo Expanded CD4+CD25+Foxp3+ Regulatory T Cells in Suppressing Murine Acute GvHD." Blood 112, no. 11 (2008): 812. http://dx.doi.org/10.1182/blood.v112.11.812.812.

Full text

Abstract:

Abstract Graft versus host disease (GVHD) is a major complication of hematopoietic stem cell transplantation resulting from donor T cell reactivity against host tissue antigens. CD4+CD25+Foxp3+ regulatory T cells (Treg) are known to be important in maintaining self tolerance and preventing autoimmunity. Using murine models of acute GVHD in which allogeneic bone marrow cells are transplanted into lethally irradiated hosts, we and others have shown that donor Treg are able to suppress GVHD induced by donor allogeneic T cells and dramatically improve survival. Treg are rare and suppression of GVH

APA, Harvard, Vancouver, ISO, and other styles

28

Tolar, Jakub, Julie Curtsinger, Ron McElmurry, et al. "Optimal Xenogeneic Adoptive Transfer of Human NK Cells: Fresh NK Cells and IL-15 Administration Are Superior to Frozen NK Cells and IL-2." Blood 120, no. 21 (2012): 346. http://dx.doi.org/10.1182/blood.v120.21.346.346.

Full text

Abstract:

Abstract Abstract 346 Expansion of NK cells after adoptive transfer is a major determinant of their anti-tumor efficacy but the mechanisms of their expansion are not completely understood. To study in vivo NK cell expansion and the relative merits of fresh versus frozen NK cell products, we used xenotransplantation of human GMP NK cell products (provided by two PACT centers funded by the NHLBI) into immune deficient mice. NOD/IL-2Rγc/Rag (NOG) that lack T-, B-, and NK cells and that have a macrophage defect that renders recipients highly amenable to human cell engraftment, were given 250 cGy t

APA, Harvard, Vancouver, ISO, and other styles

29

Montcuquet, Nicolas, Sylvain Perruche, Benjamin Shipman, et al. "Infusion of Ex-Vivo Expanded Donor T Cells To Improve Graft-Derived T-Cell Reconstitution after Allogeneic Hematopoietic Stem Cell Transplantation." Blood 110, no. 11 (2007): 3261. http://dx.doi.org/10.1182/blood.v110.11.3261.3261.

Full text

Abstract:

Abstract Limitations resulting from the reduced availability of related donors have been solved by the development of haplo-identical transplantation or by the use of cord blood as an alternative source of hematopoietic stem cells (HSC) to the bone marrow or peripheral blood. However, these kinds of transplantation remain associated with an impaired immune reconstitution, leading to an increased risk of infection and require an efficient modulation of post-transplant alloreactivity. In this setting, we and others demonstrated the possibility to control the alloreactivity by suicide gene transf

APA, Harvard, Vancouver, ISO, and other styles

30

Ohtsubo, Shin, Masakazu Ishikawa, Naosuke Kamei, et al. "The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuries." Journal of Neurosurgery 117, no. 4 (2012): 787–94. http://dx.doi.org/10.3171/2012.7.jns111503.

Full text

Abstract:

Object CD133+ cells have the potential to enhance histological and functional recovery from peripheral nerve injury. However, the number of CD133+ cells safely obtained from human peripheral blood is extremely limited. To address this issue, the authors expanded CD133+ cells derived from human peripheral blood using the serum-free expansion culture method and transplanted these ex vivo expanded cells into a model of sciatic nerve defect in rats. The purpose of this study was to determine the potential of ex vivo expanded CD133+ cells to induce or enhance the repair of injured peripheral nerves

APA, Harvard, Vancouver, ISO, and other styles

31

Grynspan, Frida, Moshe Marikovsky, Efrat Landau, et al. "Ex-Vivo Expanded Human Bone Marrow-Derived AC133+ Cells To Treat Myocardial Infarction." Blood 104, no. 11 (2004): 154. http://dx.doi.org/10.1182/blood.v104.11.154.154.

Full text

Abstract:

Abstract To examine whether ex-vivo expanded human bone marrow (BM)-derived AC133+ cells may participate in post myocardial infarction (MI) healing, we have examined the effect of the copper chelator tetraethylenepentamine (TEPA) on the ex-vivo expansion potential of BM-derived stem cell populations and the effect of the resulting ex-vivo expanded AC133+ cells in a MI animal model. AC133+ cells isolated from human BM, using the CliniMACS device, at purities greater than 90% were expanded in Teflon bags, in the presence of IL-6, TPO, Flt-3 ligand, and SCF with or without TEPA for three weeks. T

APA, Harvard, Vancouver, ISO, and other styles

32

Rosler, Elen S., John E. Brandt, John Chute, and Ronald Hoffman. "An in vivo competitive repopulation assay for various sources of human hematopoietic stem cells." Blood 96, no. 10 (2000): 3414–21. http://dx.doi.org/10.1182/blood.v96.10.3414.

Full text

Abstract:

Abstract The marrow repopulating potential (MRP) of different sources of human hematopoietic stem cells (HSCs) was directly compared using an in vivo assay in which severe combined immunodeficient disease (SCID) mice were implanted with human fetal bones. HSCs from 2 human lymphocyte antigen (HLA)-mismatched donors were injected individually or simultaneously into the fetal bones of a 3rd distinct HLA type and donor and recipient myeloid and lymphoid cells were identified after 8 to 10 weeks. The study compared the MRP of umbilical cord blood (CB) and adult bone marrow (ABM) CD34+ cells as wel

APA, Harvard, Vancouver, ISO, and other styles

33

Rosler, Elen S., John E. Brandt, John Chute, and Ronald Hoffman. "An in vivo competitive repopulation assay for various sources of human hematopoietic stem cells." Blood 96, no. 10 (2000): 3414–21. http://dx.doi.org/10.1182/blood.v96.10.3414.h8003414_3414_3421.

Full text

Abstract:

The marrow repopulating potential (MRP) of different sources of human hematopoietic stem cells (HSCs) was directly compared using an in vivo assay in which severe combined immunodeficient disease (SCID) mice were implanted with human fetal bones. HSCs from 2 human lymphocyte antigen (HLA)-mismatched donors were injected individually or simultaneously into the fetal bones of a 3rd distinct HLA type and donor and recipient myeloid and lymphoid cells were identified after 8 to 10 weeks. The study compared the MRP of umbilical cord blood (CB) and adult bone marrow (ABM) CD34+ cells as well as graf

APA, Harvard, Vancouver, ISO, and other styles

34

KO, YUNMI, та Jun Ah Lee. "Effect of CD3/CD28 activator on the cytotoxicity of human ex-vivo expanded γδ T cells against osteosarcoma cells". Journal of Immunology 204, № 1_Supplement (2020): 88.1. http://dx.doi.org/10.4049/jimmunol.204.supp.88.1.

Full text

Abstract:

Abstract Background We investigated the effect of CD3/CD28 activator on the ex-vivo expansion of human γδ T cells and their cytotoxic effect against osteosarcoma cells. Methods Peripheral blood mononuclear cells from healthy donors were culture in three different conditions for 10 days: basal expansion media (COM media), COM media with zoledronate+hrIL-2 (EX media), and EX media with CD3/CD28 activator (EX28 media). Percentage of expanded γδ T cells was analyzed by flow cytometry. IFN-γ and TNF-α secreted in the culture media was measured using ELISA assay. Cytotoxicity of γδ T cells against K

APA, Harvard, Vancouver, ISO, and other styles

35

Ong, Li Ming, Xiubo Fan, Pak Yan Chu, et al. "Kinetics of Engraftment and Graft Versus Host Disease After Cotransplantation of Ex Vivo Expanded and Unexpanded Cord Blood Units In Immunodeficient Mice." Blood 116, no. 21 (2010): 3722. http://dx.doi.org/10.1182/blood.v116.21.3722.3722.

Full text

Abstract:

Abstract Abstract 3722 Ex vivo expansion of cord blood (CB) hematopoietic stem cells (HSCs) and cotransplantation of two CB units can enhance applicability of CB transplants to adult patients. This is the first study on cotransplantation of ex vivo expanded and unexpanded human CB units in immunodeficient mice, simulating conditions for ex vivo CB expansion clinical trials. CB units were cultured in serum-free medium supplemented with Stem Cell Factor, Flt-3 ligand, Thrombopoietin and Insulin Growth Factor Binding Protein-2 with mesenchymal stromal co-culture. Cotransplantation of unexpanded a

APA, Harvard, Vancouver, ISO, and other styles

36

Sasaki, Shuji, Toyoshi Inoguchi, Koichiro Muta, et al. "Therapeutic angiogenesis by ex vivo expanded erythroid progenitor cells." American Journal of Physiology-Heart and Circulatory Physiology 292, no. 1 (2007): H657—H665. http://dx.doi.org/10.1152/ajpheart.00343.2006.

Full text

Abstract:

Recent reports have demonstrated that erythroid progenitor cells contain and secrete various angiogenic cytokines. Here, the impact of erythroid colony-forming cell (ECFC) implantation on therapeutic angiogenesis was investigated in murine models of hindlimb ischemia. During the in vitro differentiation, vascular endothelial growth factor (VEGF) secretion by ECFCs was observed from day 3 (burst-forming unit erythroid cells) to day 10 (erythroblasts). ECFCs from day 5 to day 7 (colony-forming unit erythroid cells) showed the highest VEGF productivity, and day 6 ECFCs were used for the experimen

APA, Harvard, Vancouver, ISO, and other styles

37

Davidson-Moncada, Jan K., Richard Childs, Cynthia E. Dunbar, et al. "Rhesus Macaque NK Cells Expanded Ex Vivo Undergo Similar Phenotypic and Functional Changes Observed With Expanded Human NK Cells Providing An Excellent Model To Optimize Adoptive NK Cell Transfer." Blood 122, no. 21 (2013): 2028. http://dx.doi.org/10.1182/blood.v122.21.2028.2028.

Full text

Abstract:

Abstract Although pilot clinical trials have shown adoptive NK cell transfer can result in tumor regression in humans with cancer, additional insight from animal models are needed to optimize NK cell proliferation in vivo as well as to improve their homing and retention in the tumor microenvironment. Mice have provided fundamental insights into NK cell biology, although significant divergence from humans as a consequence of evolution limits their usefulness to optimize adoptive transfer of ex vivo expanded NK cells in humans. Rhesus macaques (RM) express orthologues to most human MHC class I a

APA, Harvard, Vancouver, ISO, and other styles

38

Blinova, Varvara G., Natalia S. Novachly, Sofya N. Gippius, et al. "Phenotypical and Functional Characteristics of Human Regulatory T Cells during Ex Vivo Maturation from CD4+ T Lymphocytes." Applied Sciences 11, no. 13 (2021): 5776. http://dx.doi.org/10.3390/app11135776.

Full text

Abstract:

Regulatory T cells (Tregs) participate in the negative regulation of inflammatory reactions by suppressing effector cells. In a number of autoimmune disorders, the suppressive function and/or the number of Tregs is compromised. The lack of active functioning Tregs can be restored with adoptive transfer of expanded ex vivo autologous Tregs. In our study, we traced the differentiation and maturation of Tregs CD4+CD25+FoxP3+CD127low over 7 days of cultivation from initial CD4+ T cells under ex vivo conditions. The resulting ex vivo expanded cell population (eTregs) demonstrated the immune profile

APA, Harvard, Vancouver, ISO, and other styles

39

Di Bella, C. "3D BIOPRINTING OF ARTICULAR CARTILAGE FOR IN VIVO APPLICATION." Orthopaedic Proceedings 105-B, SUPP_2 (2023): 52. http://dx.doi.org/10.1302/1358-992x.2023.2.052.

Full text

Abstract:

3D printing and Bioprinting technologies are becoming increasingly popular in surgery to provide a solution for the regeneration of healthy tissues. The aim of our project is the regeneration of articular cartilage via bioprinting means, to manage isolated chondral defects.Chrondrogenic hydrogel (chondrogel: GelMa + TGF-b3 and BMP6) was prepared and sterilised in our lab following our standard protocols. Human adipose-derived mesenchymal stem cells were harvested from the infrapatellar fat pad of patients undergoing total knee joint replacements and incorporated in the hydrogel according to ou

APA, Harvard, Vancouver, ISO, and other styles

40

Ласюков, Е. А., Е. П. Вашкевич, А. С. Мухаметшина, М. А. Новикова, Н. В. Липай, and Т. В. Шман. "Comparative Analysis of Cryopreservation Methods for Expanded Ex Vivo Natural Killer Cells: Recommendations for Optimizing Research Technologies." Лабораторная диагностика. Восточная Европа, no. 4 (December 30, 2021): 450–57. http://dx.doi.org/10.34883/pi.2021.10.4.006.

Full text

Abstract:

Цель. На основании сравнительного анализа известных технологий криоконсервации экспансированных ex vivo естественных киллерных клеток рекомендовать к использованию наиболее эффективные в отношении сохранения жизнеспособности ЕК-клеток. Материалы и методы. В исследование было включено 10 образцов экспансированных ex vivo ЕК-клеток человека, замороженных в 4 типах криопротекторных смесей с использованием различных технологий заморозки и хранившихся в течение 30 суток в парах жидкого азота. Сохранность образцов после размораживания оценивали по показателям жизнеспособности, цитотоксической активн

APA, Harvard, Vancouver, ISO, and other styles

41

Albella, Beatriz, José C. Segovia, and Juan A. Bueren. "Does the Granulocyte-Macrophage Colony-Forming Unit Content in Ex Vivo–Expanded Grafts Predict the Recovery of the Recipient Leukocytes?" Blood 90, no. 1 (1997): 464–70. http://dx.doi.org/10.1182/blood.v90.1.464.

Full text

Abstract:

Abstract We have investigated the leukocyte-repopulating-predictive value of granulocyte-macrophage colony-forming unit (CFU-GM) analyses in ex vivo–expanded versus fresh murine bone marrow (BM) grafts. After the transplantation of graded numbers of normal BM cells (from 15 to 5 × 103 CFU-GMs/mice), a dose-dependent increase in the recipient leukocytes was observed between the first and third weeks posttransplantation. During these stages, increases in the graft size of 100-fold improved the leukocyte counts up to 30-fold and shortened the leukopenia period by 5 to 11 days, depending on the le

APA, Harvard, Vancouver, ISO, and other styles

42

Albella, Beatriz, José C. Segovia, and Juan A. Bueren. "Does the Granulocyte-Macrophage Colony-Forming Unit Content in Ex Vivo–Expanded Grafts Predict the Recovery of the Recipient Leukocytes?" Blood 90, no. 1 (1997): 464–70. http://dx.doi.org/10.1182/blood.v90.1.464.464_464_470.

Full text

Abstract:

We have investigated the leukocyte-repopulating-predictive value of granulocyte-macrophage colony-forming unit (CFU-GM) analyses in ex vivo–expanded versus fresh murine bone marrow (BM) grafts. After the transplantation of graded numbers of normal BM cells (from 15 to 5 × 103 CFU-GMs/mice), a dose-dependent increase in the recipient leukocytes was observed between the first and third weeks posttransplantation. During these stages, increases in the graft size of 100-fold improved the leukocyte counts up to 30-fold and shortened the leukopenia period by 5 to 11 days, depending on the leukocyte t

APA, Harvard, Vancouver, ISO, and other styles

43

Shourian, Mitra, Benoite Bourdin, and Helene Decaluwe. "Inhibition of JAK/STAT signaling sustain stem-like exhausted CD8+ T lymphocytes with enhanced antitumor effects." Journal of Immunology 206, no. 1_Supplement (2021): 58.06. http://dx.doi.org/10.4049/jimmunol.206.supp.58.06.

Full text

Abstract:

Abstract T-cell exhaustion and reduced memory potential is an obstacle to successful adoptive cell therapy (ACT) of cancer. Ex-vivo expansion protocols require sustained TCR and cytokine stimulation that limit ACT efficacy and long-term persistence. We have shown that IL-2 and IL-15, JAK-STAT dependent-cytokines used in ACT expansion protocols, induce CD8+ T-cell exhaustion both in vitro and in vivo following chronic viral infection. We hypothesized that blocking JAK-STAT signaling during ex-vivo expansion protocols will limit T-cell exhaustion and sustain stemness of adoptively transferred ce

APA, Harvard, Vancouver, ISO, and other styles

44

Holyoake, TL, MG Freshney, L. McNair, et al. "Ex vivo expansion with stem cell factor and interleukin-11 augments both short-term recovery posttransplant and the ability to serially transplant marrow." Blood 87, no. 11 (1996): 4589–95. http://dx.doi.org/10.1182/blood.v87.11.4589.bloodjournal87114589.

Full text

Abstract:

The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-11 (IL- 11) produced a 50-fold amplification of clonogenic multi

APA, Harvard, Vancouver, ISO, and other styles

45

Levy, Emily R., Joseph A. Clara, Robert N. Reger, David S. J. Allan, and Richard W. Childs. "RNA-Seq Analysis Reveals CCR5 as a Key Target for CRISPR Gene Editing to Regulate In Vivo NK Cell Trafficking." Cancers 13, no. 4 (2021): 872. http://dx.doi.org/10.3390/cancers13040872.

Full text

Abstract:

A growing number of natural killer (NK) cell-based immunotherapy trials utilize ex vivo expansion to grow and activate allogenic and autologous NK cells prior to administration to patients with malignancies. Recent data in both murine and macaque models have shown that adoptively infused ex vivo expanded NK cells have extensive trafficking into liver tissue, with relatively low levels of homing to other sites where tumors often reside, such as the bone marrow or lymph nodes. Here, we evaluated gene and surface expression of molecules involved in cellular chemotaxis in freshly isolated human NK

APA, Harvard, Vancouver, ISO, and other styles

46

Park, Ga-Bin, Min-Jung Kim, Elena A. Vasileva та ін. "Echinochrome A Promotes Ex Vivo Expansion of Peripheral Blood-Derived CD34+ Cells, Potentially through Downregulation of ROS Production and Activation of the Src-Lyn-p110δ Pathway". Marine Drugs 17, № 9 (2019): 526. http://dx.doi.org/10.3390/md17090526.

Full text

Abstract:

Intracellular reactive oxygen species (ROS) play an important role in the proliferation and differentiation of hematopoietic stem and progenitor cells (HSPCs). HSPCs are difficult to be expanded ex vivo while maintaining their stemness when they are exposed to oxidative damage after being released from the bone marrow. There have been efforts to overcome this limitation by using various cytokine cocktails and antioxidants. In this study, we investigated the effects of echinochrome A (Ech A)-a well-established and non-toxic antioxidant-on the ex vivo expansion of HSPCs by analyzing a CD34+ cell

APA, Harvard, Vancouver, ISO, and other styles

47

Gregory, S. R., C. Fife, J. Williams, et al. "P08.02 Harnessing T cells to target brain metastasis." Neuro-Oncology 23, Supplement_2 (2021): ii26. http://dx.doi.org/10.1093/neuonc/noab180.089.

Full text

Abstract:

Abstract BACKGROUND Up to 60% of melanoma patients develop brain metastases (BrM). These patients have a poor prognosis and limited treatment options. Immune checkpoint inhibitors (ICI) targeting Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) and Programmed cell death protein-1 (PD-1) have revolutionized the treatment of melanoma and their efficacy has been also demonstrated in melanoma BrM. Our group previously demonstrated that ICI (combined α-PD-1 and α-CTLA-4) enhances chemokine-dependent infiltration of cytotoxic T lymphocytes (CTLs) into melanoma BrMs in preclinical models, accompa

APA, Harvard, Vancouver, ISO, and other styles

48

C, Abisha Crystal, Saravanabhavan Thangavel, Shaji Ramachandran Velayudhan, et al. "SMALL Molecules Mediated Hematopoietic STEM and Progenitor CELLS Expansion for GENE Editing Application." Blood 132, Supplement 1 (2018): 5803. http://dx.doi.org/10.1182/blood-2018-99-120262.

Full text

Abstract:

Abstract Genome editing of Hematopoietic stem Cells has revolutionized the treatment strategies for genetic disorders. Despite this, it still remains a great challenge as hematopoietic stem cells tend to lose its stem-ness during the ex vivo culture and gene editing process. The need for large dose of CD34+ HSPCs for manipulation makes it a seemingly difficult strategy. Recent works suggest that the potential effects of small molecules in expanding cord blood HSPCs ex vivo promoting self-renewal and delaying differentiation. We screened several reported small molecules to identify a condition

APA, Harvard, Vancouver, ISO, and other styles

49

Xing, Dongxia, Wendy Fang, William K. Decker, et al. "Ex Vivo Expansion of Cord Blood NK Cell Have In Vivo Efficacy Against Leukemia." Blood 110, no. 11 (2007): 2741. http://dx.doi.org/10.1182/blood.v110.11.2741.2741.

Full text

Abstract:

Abstract Introduction Allogeneic stem cell transplantation has demonstrated the ability to prevent leukemic relapse via an immune-mediated graft-vs.-leukemia effect. Natural killer (NK) cells have been shown to comprise a significant component of this anti-leukemia effect and have been reported to enhance engraftment and reduce graft-vs.-host disease. Ex-vivo expansion of peripheral blood-derived NK cells has been demonstrated. Cord blood (CB) is a promising alternate source of NK cells with enhanced cytokine/antigen-responsiveness, proliferation and increasing availability. We studied expansi

APA, Harvard, Vancouver, ISO, and other styles

50

Ko, Yunmi, Yeon Ho Jeong та Jun Ah Lee. "Therapeutic Potential of Ex Vivo Expanded γδ T Cells against Osteosarcoma Cells". Cells 11, № 14 (2022): 2164. http://dx.doi.org/10.3390/cells11142164.

Full text

Abstract:

Immunotherapy is an attractive therapeutic strategy for the treatment of osteosarcoma (OS). The unique features of γδ T cells have made them popular for cancer immunotherapy. Here, we expanded γδ T cells using human peripheral blood mononuclear cells (PBMCs) and investigated their therapeutic potential against OS cells. PBMCs from healthy donors were cultured for 10 days with CON medium (unstimulated control); EX media, CON with recombinant human interleukin-2 (rhIL-2) and zoledronate; and EX28 media, CON with rhIL-2, zoledronate, and CD3/CD28 activator. The expanded γδ T cells were isolated b

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!