Journal articles: 'Ex vivo hair growth'

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Hundt, Jennifer E., Stephanie Sass, Wolfgang Funk, et al. "Mineralocorticoid Receptor Antagonists Stimulate Human Hair Growth ex vivo." Skin Pharmacology and Physiology 32, no. 6 (2019): 344–48. http://dx.doi.org/10.1159/000501729.

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Fernández-Martos, Sandra, María Calvo-Sánchez, Karla García-Alonso, Begoña Castro, Bita Hashtroody, and Jesús Espada. "Sustained Human Hair Follicle Growth Ex Vivo in a Glycosaminoglycan Hydrogel Matrix." International Journal of Molecular Sciences 20, no. 7 (2019): 1741. http://dx.doi.org/10.3390/ijms20071741.

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Glycosaminoglycans (GAGs) and associated proteoglycans have important functions in homeostatic maintenance and regenerative processes (e.g., wound repair) of the skin. However, little is known about the role of these molecules in the regulation of the hair follicle cycle. Here we report that growing human hair follicles ex vivo in a defined GAG hydrogel mimicking the dermal matrix strongly promotes sustained cell survival and maintenance of a highly proliferative phenotype in the hair bulb and suprabulbar regions. This significant effect is associated with the activation of WNT/β-catenin signaling targets (CCDN1, AXIN2) and with the expression of stem cell markers (CK15, CD34) and growth factors implicated in the telogen/anagen transition (TGFβ2, FGF10). As a whole, these results point to the dermal GAG matrix as an important component in the regulation of the human hair follicle growth cycle, and to GAG-based hydrogels as potentially relevant modulators of this process both in vitro and in vivo.

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Kwon, Oh Sang, Jun Kyu Oh, Mi Hyang Kim, et al. "Human hair growth ex vivo is correlated with in vivo hair growth: selective categorization of hair follicles for more reliable hair follicle organ culture." Archives of Dermatological Research 297, no. 8 (2005): 367–71. http://dx.doi.org/10.1007/s00403-005-0619-z.

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Haslam, Iain S., Jonathan A. Hardman, and Ralf Paus. "Topically Applied Nicotinamide Inhibits Human Hair Follicle Growth Ex Vivo." Journal of Investigative Dermatology 138, no. 6 (2018): 1420–22. http://dx.doi.org/10.1016/j.jid.2017.12.019.

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Bacqueville, Daniel, Marguerite Lévêque, Camille Mas, et al. "New Plant Extracts Exert Complementary Anti‐Hair Loss Properties in Human In Vitro and Ex Vivo Models." Journal of Cosmetic Dermatology 23, S5 (2024): 1–11. http://dx.doi.org/10.1111/jocd.16616.

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ABSTRACTBackgroundHair loss is linked to dysfunction of the growth (anagen), regression (catagen) and rest (telogen) phases of the hair follicle (HF) cycle.AimsTo evaluate the effects of a Silybum marianum extract (SME), manganese PCA (MnPCA), and a Lespedeza capitata extract (LCE) on markers of hair growth and anchorage in human follicle dermal papilla cells (HFDPCs), and to investigate the ability of a topical serum containing these active ingredients to improve HF growth in an ex vivo human scalp skin model.MethodsIn HFDPCs, we assessed receptor tyrosine kinase phosphorylation and Wnt/β‐catenin pathway activation; quantified versican, vascular endothelial growth factor (VEGF) and Dickkopf‐1 (DDK1) secretion; and evaluated 5α‐reductase (5αR) activity. Using scalp skin biopsies from two female donors, we measured hair shaft elongation, analyzed hair matrix keratinocyte proliferation and apoptosis, and determined HF cycle stage and score.ResultsCompared to untreated HFDPCs, SME upregulated phosphorylation of growth factor receptors (EGFR:1.9 × and PDGFR: 2.8 ×) and their downstream effectors (ERK, GSK3, Akt, and STAT: 1.2–2.0 ×); MnPCA enhanced versican (33.0 ×) and VEGF (3.3 ×) secretion, and stimulated the Wnt/β‐catenin pathway (+80%); and LCE reduced DKK1 secretion (−72%) and 5αR activity (dihydrotestosterone/testosterone ratio: −60%). Compared to untreated scalp skin biopsies, the serum enhanced hair shaft elongation (+102%), and significantly prolonged the anagen phase by improving hair cycle scores and stimulating hair matrix keratinocyte proliferation (+58%).ConclusionsSME, MnPCA, and LCE displayed complementary anti‐hair loss properties. The serum combining these active ingredients may be useful in hair loss treatment.

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Alam, Majid, Diana A. Below, Jérémy Chéret, et al. "Growth Hormone Operates as a Neuroendocrine Regulator of Human Hair Growth Ex Vivo." Journal of Investigative Dermatology 139, no. 7 (2019): 1593–96. http://dx.doi.org/10.1016/j.jid.2018.12.022.

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McElwee, Kevin J., Andrea Huth, Sabine Kissling, and Rolf Hoffmann. "Macrophage-Stimulating Protein Promotes Hair Growth Ex Vivo and Induces Anagen from Telogen Stage Hair Follicles In Vivo." Journal of Investigative Dermatology 123, no. 1 (2004): 34–40. http://dx.doi.org/10.1111/j.0022-202x.2004.22712.x.

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Kim, Jaeyoon, Jae young Shin, Yun-Ho Choi, et al. "Hair Thickness Growth Effect of Adenosine Complex in Male-/Female-Patterned Hair Loss via Inhibition of Androgen Receptor Signaling." International Journal of Molecular Sciences 25, no. 12 (2024): 6534. http://dx.doi.org/10.3390/ijms25126534.

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Aging (senescence) is an unavoidable biological process that results in visible manifestations in all cutaneous tissues, including scalp skin and hair follicles. Previously, we evaluated the molecular function of adenosine in promoting alopecia treatment in vitro. To elucidate the differences in the molecular mechanisms between minoxidil (MNX) and adenosine, gene expression changes in dermal papilla cells were examined. The androgen receptor (AR) pathway was identified as a candidate target of adenosine for hair growth, and the anti-androgenic activity of adenosine was examined in vitro. In addition, ex vivo examination of human hair follicle organ cultures revealed that adenosine potently elongated the anagen stage. According to the severity of alopecia, the ratio of the two peaks (terminal hair area/vellus hair area) decreased continuously. We further investigated the adenosine hair growth promoting effect in vivo to examine the hair thickness growth effects of topical 5% MNX and the adenosine complex (0.75% adenosine, 1% penthenol, and 2% niacinamide; APN) in vivo. After 4 months of administration, both the MNX and APN group showed significant increases in hair density (MNX + 5.01% (p < 0.01), APN + 6.20% (p < 0.001)) and thickness (MNX + 5.14% (p < 0.001), APN + 10.32% (p < 0.001)). The inhibition of AR signaling via adenosine could have contributed to hair thickness growth. We suggest that the anti-androgenic effect of adenosine, along with the evaluation of hair thickness distribution, could help us to understand hair physiology and to investigate new approaches for drug development.

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Le Riche, A., J. Nienhaus, M. Sousa, et al. "1420 Ashwagandha-derived exosome-like nanoparticles promote human hair growth ex vivo." Journal of Investigative Dermatology 143, no. 5 (2023): S243. http://dx.doi.org/10.1016/j.jid.2023.03.1436.

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Trivedi, Dahryn, and Snehasis Jana. "In vivo Hair Growth Promotion Efficacy of Biofield Energy Treatment in C57BL/6 Mice." Letters in Health and Biological Sciences 3, no. 2 (2018): 51–55. https://doi.org/10.15436/2475-6245.18.2031.

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Hair loss directly impacts people’s social interactions and also affects psychological well-being. Hair fall has been a rising trend nowadays due to dynamic lifestyle and pollution. In this context,the present study was performed to identify the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (mixture of herbal ex-tracts of Phyllantus emblica and Eclipta alba in 1:1 ratio) on the telogen skin of mice for the assessment of haircell growth and development in vivo. The test item was divided into two parts. One part was denoted as the untreated test item without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Dahryn Trivedi. The study parameters like anagen induction and visual melanogenesis were performed using skin biopsy technique. The experimental results of the untreated and Biofield Energy Treated test item groups showed hair growth with 50% and 60%, respectively on dorsal clipped of skin after topical application. Besides, the Biofield Energy Treated test item group exhibited 60% melanogenesis after biopsy analysis in mice skin at the end of the experiment. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased hair growth and melanogenesis. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment could be useful as a hair growth promoter for various treatments of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer, etc. <strong>Source</strong>: https://www.trivedieffect.com/science/in-vivo-hair-growth-promotion-efficacy-of-biofield-energy-treatment-in-c57bl6-mice/ https://www.ommegaonline.org/article-details/In-vivo-Hair-Growth-Promotion-Efficacy-of-Biofield-Energy-Treatment-in-C57BL6-Mice/2031

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Oláh, Attila, Jennifer Gherardini, Marta Bertolini, et al. "The Thyroid Hormone Analogue KB2115 (Eprotirome) Prolongs Human Hair Growth (Anagen) Ex Vivo." Journal of Investigative Dermatology 136, no. 8 (2016): 1711–14. http://dx.doi.org/10.1016/j.jid.2016.03.033.

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Upadhyay, Ratna, Mihir C. Gadani, and Sneha Badak. "Evaluating the hair regenerative potential of plant-based hair serum formulation: a comparative study." International Journal of Scientific Reports 11, no. 1 (2024): 20–24. https://doi.org/10.18203/issn.2454-2156.intjscirep20243799.

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Background: Hair loss, a multifactorial condition influenced by factors such as aging, hormonal imbalances, and genetics, has traditionally been treated with synthetic products like minoxidil and finasteride. However, these treatments often come with significant side effects, highlighting the need for safer and more effective alternatives. This study investigates the efficacy and safety of OZiva+ advanced hair growth serum, a formulation containing 3% Redensyl® and 1% Lindera root extract, as a potential treatment for hair loss. Methods: The serum's hair growth-promoting effects were evaluated using the ex vivo Philpott test, involving hair plucks from androgenetic alopecia patients, and its safety was assessed through a clinical patch test on human volunteers. Results: The results demonstrated a significant increase in hair follicle length with the OZiva+ advanced hair growth serum, comparable to 1% minoxidil. Additionally, the serum showed no irritation during the patch study, indicating its safety for use. Conclusions: The findings suggest that OZiva+ advanced hair growth serum is a promising alternative to traditional hair loss treatments, offering comparable efficacy with fewer side effects. This natural formulation, supported by the synergistic action of Redensyl® and Lindera root extract, holds potential for further development as a safer, more effective solution for hair regrowth and overall hair health.

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Fernandes, Bruno, Teresa Matamá, Andreia C. Gomes, and Artur Cavaco-Paulo. "Cyclosporin A-loaded poly(d,l-lactide) nanoparticles: a promising tool for treating alopecia." Nanomedicine 15, no. 15 (2020): 1459–69. http://dx.doi.org/10.2217/nnm-2020-0089.

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Background: Alopecia treatments are scarce and lack efficacy. Cyclosporin A (CsA) has hair growth-inducing properties but its poor cutaneous absorption undermines its use in topical treatments. Aim: Development of a new potential topical treatment of alopecia with CsA. Materials & methods: CsA-loaded poly(d,l-lactide) (PLA) nanoparticles were obtained and characterized. Skin permeation was evaluated in ex vivo porcine skin. Results: Nanoparticles with good physicochemical stability increased CsA skin permeation/hair follicles accumulation, compared with a noncolloidal formulation. CsA biocompatibility in NCTC2455 keratinocytes (reference skin cell line) was clearly improved when encapsulated in PLA nanoparticles. Conclusion: This work fosters further in vivo investigation of CsA-loaded PLA nanoparticles as a promising new strategy to treat alopecia, a very traumatic, possibly autoimmune, disease.

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Choi, Joon Yong, Min Young Boo, and Yong Chool Boo. "Can Plant Extracts Help Prevent Hair Loss or Promote Hair Growth? A Review Comparing Their Therapeutic Efficacies, Phytochemical Components, and Modulatory Targets." Molecules 29, no. 10 (2024): 2288. http://dx.doi.org/10.3390/molecules29102288.

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This narrative review aims to examine the therapeutic potential and mechanism of action of plant extracts in preventing and treating alopecia (baldness). We searched and selected research papers on plant extracts related to hair loss, hair growth, or hair regrowth, and comprehensively compared the therapeutic efficacies, phytochemical components, and modulatory targets of plant extracts. These studies showed that various plant extracts increased the survival and proliferation of dermal papilla cells in vitro, enhanced cell proliferation and hair growth in hair follicles ex vivo, and promoted hair growth or regrowth in animal models in vivo. The hair growth-promoting efficacy of several plant extracts was verified in clinical trials. Some phenolic compounds, terpenes and terpenoids, sulfur-containing compounds, and fatty acids were identified as active compounds contained in plant extracts. The pharmacological effects of plant extracts and their active compounds were associated with the promotion of cell survival, cell proliferation, or cell cycle progression, and the upregulation of several growth factors, such as IGF-1, VEGF, HGF, and KGF (FGF-7), leading to the induction and extension of the anagen phase in the hair cycle. Those effects were also associated with the alleviation of oxidative stress, inflammatory response, cellular senescence, or apoptosis, and the downregulation of male hormones and their receptors, preventing the entry into the telogen phase in the hair cycle. Several active plant extracts and phytochemicals stimulated the signaling pathways mediated by protein kinase B (PKB, also called AKT), extracellular signal-regulated kinases (ERK), Wingless and Int-1 (WNT), or sonic hedgehog (SHH), while suppressing other cell signaling pathways mediated by transforming growth factor (TGF)-β or bone morphogenetic protein (BMP). Thus, well-selected plant extracts and their active compounds can have beneficial effects on hair health. It is proposed that the discovery of phytochemicals targeting the aforementioned cellular events and cell signaling pathways will facilitate the development of new targeted therapies for alopecia.

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Gherardini, J., T. Rouille, M. Fehrholz, et al. "743 Hair follicles can “taste”: Stevioside stimulation of the bitter taste receptor, TAS2R4, inhibits human hair growth ex vivo." Journal of Investigative Dermatology 142, no. 8 (2022): S128. http://dx.doi.org/10.1016/j.jid.2022.05.755.

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Suzuki, T., F. Scala, J. Gherardini, et al. "033 IL-15 is an unexpected guardian of hair follicle immune privilege and promotes human hair growth ex vivo." Journal of Investigative Dermatology 141, no. 5 (2021): S6. http://dx.doi.org/10.1016/j.jid.2021.02.049.

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Horesh, Elijah J., Jérémy Chéret, and Ralf Paus. "Growth Hormone and the Human Hair Follicle." International Journal of Molecular Sciences 22, no. 24 (2021): 13205. http://dx.doi.org/10.3390/ijms222413205.

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Ever since the discoveries that human hair follicles (HFs) display the functional peripheral equivalent of the hypothalamic-pituitary-adrenal axis, exhibit elements of the hypothalamic-pituitary-thyroid axis, and even generate melatonin and prolactin, human hair research has proven to be a treasure chest for the exploration of neurohormone functions. However, growth hormone (GH), one of the dominant neurohormones of human neuroendocrine physiology, remains to be fully explored in this context. This is interesting since it has long been appreciated clinically that excessive GH serum levels induce distinct human skin pathology. Acromegaly, or GH excess, is associated with hypertrichosis, excessive androgen-independent growth of body hair, and hirsutism in females, while dysfunctional GH receptor-mediated signaling (Laron syndrome) is associated with alopecia and prominent HF defects. The outer root sheath keratinocytes have recently been shown to express functional GH receptors. Furthermore, and contrary to its name, recombinant human GH is known to inhibit female human scalp HFs’ growth ex vivo, likely via stimulating the expression of the catagen-inducing growth factor, TGF-β2. These limited available data encourage one to systematically explore the largely uncharted role of GH in human HF biology to uncover nonclassical functions of this core neurohormone in human skin physiology.

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Rajendran, Ramya Lakshmi, Prakash Gangadaran, Mi Hee Kwack, et al. "Engineered Nanovesicles from Fibroblasts Modulate Dermal Papillae Cells In Vitro and Promote Human Hair Follicle Growth Ex Vivo." Cells 11, no. 24 (2022): 4066. http://dx.doi.org/10.3390/cells11244066.

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Alopecia is a common medical condition affecting both sexes. Dermal papilla (DP) cells are the primary source of hair regeneration in alopecia patients. Therapeutic applications of extracellular vesicles (EVs) are restricted by low yields, high costs, and their time-consuming collection process. Thus, engineered nanovesicles (eNVs) have emerged as suitable therapeutic biomaterials in translational medicine. We isolated eNVs by the serial extrusion of fibroblasts (FBs) using polycarbonate membrane filters and serial and ultracentrifugation. We studied the internalization, proliferation, and migration of human DP cells in the presence and absence of FB-eNVs. The therapeutic potential of FB-eNVs was studied on ex vivo organ cultures of human hair follicles (HFs) from three human participants. FB-eNVs (2.5, 5, 7.5, and 10 µg/mL) significantly enhanced DP cell proliferation, with the maximum effect observed at 7.5 µg/mL. FB-eNVs (5 and 10 µg/mL) significantly enhanced the migration of DP cells at 36 h. Western blotting results suggested that FB-eNVs contain vascular endothelial growth factor (VEGF)-a. FB-eNV treatment increased the levels of PCNA, pAKT, pERK, and VEGF-receptor-2 (VEGFR2) in DP cells. Moreover, FB-eNVs increased the human HF shaft size in a short duration ex vivo. Altogether, FB-eNVs are promising therapeutic candidates for alopecia.

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Lim, Hye Won, Hak Joong Kim, Chae Young Jeon, et al. "Hair Growth Promoting Effects of 15-Hydroxyprostaglandin Dehydrogenase Inhibitor in Human Follicle Dermal Papilla Cells." International Journal of Molecular Sciences 25, no. 13 (2024): 7485. http://dx.doi.org/10.3390/ijms25137485.

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Prostaglandin E2 (PGE2) is known to be effective in regenerating tissues, and bimatoprost, an analog of PGF2α, has been approved by the FDA as an eyelash growth promoter and has been proven effective in human hair follicles. Thus, to enhance PGE2 levels while improving hair loss, we found dihydroisoquinolinone piperidinylcarboxy pyrazolopyridine (DPP), an inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), using DeepZema®, an AI-based drug development program. Here, we investigated whether DPP improved hair loss in human follicle dermal papilla cells (HFDPCs) damaged by dihydrotestosterone (DHT), which causes hair loss. We found that DPP enhanced wound healing and the expression level of alkaline phosphatase in DHT-damaged HFDPCs. We observed that DPP significantly down-regulated the generation of reactive oxygen species caused by DHT. DPP recovered the mitochondrial membrane potential in DHT-damaged HFDPCs. We demonstrated that DPP significantly increased the phosphorylation levels of the AKT/ERK and activated Wnt signaling pathways in DHT-damaged HFDPCs. We also revealed that DPP significantly enhanced the size of the three-dimensional spheroid in DHT-damaged HFDPCs and increased hair growth in ex vivo human hair follicle organ culture. These data suggest that DPP exhibits beneficial effects on DHT-damaged HFDPCs and can be utilized as a promising agent for improving hair loss.

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Trivedi, Mahendra Kumar, and Snehasis Jana. "Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture." JOURNAL OF DERMATOLOGICAL RESEARCH AND THERAPY 1, no. 3 (2019): 12–19. https://doi.org/10.14302/issn.2471-2175.jdrt-18-2520.

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Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc. <strong>Source:</strong> https://www.trivedieffect.com/science/assessment-of-hair-growth-treatment-with-the-consciousness-energy-healing-treated-williams-medium-e-using-mouse-vibrissae-hair-follicle-organ-culture/ https://openaccesspub.org/jdrt/article/957

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Bassino, Eleonora, Franco Gasparri, and Luca Munaron. "Protective Role of Nutritional Plants Containing Flavonoids in Hair Follicle Disruption: A Review." International Journal of Molecular Sciences 21, no. 2 (2020): 523. http://dx.doi.org/10.3390/ijms21020523.

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Hair loss is a disorder in which the hair falls out from skin areas such as the scalp and the body. Several studies suggest the use of herbal medicine to treat related disorders, including alopecia. Dermal microcirculation is essential for hair maintenance, and an insufficient blood supply can lead to hair follicles (HF) diseases. This work aims to provide an insight into the ethnohistorical records of some nutritional compounds containing flavonoids for their potential beneficial features in repairing or recovering from hair follicle disruption. We started from a query for “alopecia” OR “hair loss” AND “Panax ginseng C.A. Mey.“ (or other six botanicals) terms included in Pubmed and Web of Sciences articles. The activities of seven common botanicals introduced with diet (Panax ginseng C.A. Mey., Malus pumila Mill cultivar Annurca, Coffea arabica, Allium sativum L., Camellia sinensis (L.) Kuntze, Rosmarinum officinalis L., Capsicum annum L.) are discussed, which are believed to reduce the rate of hair loss or stimulate new hair growth. In this review, we pay our attention on the molecular mechanisms underlying the bioactivity of the aforementioned nutritional compounds in vivo, ex vivo and in vitro studies. There is a need for systematic evaluation of the most commonly used plants to confirm their anti-hair loss power, identify possible mechanisms of action, and recommend their best adoption.

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Agramunt, J., J. Cheret, J. Gherardini, F. Jimenez, and R. Paus. "503 A new chemosensory control of human hair growth: Olfactory receptor OR10J1 stimulation promotes scalp hair follicle growth and progenitor cell generation ex vivo." Journal of Investigative Dermatology 144, no. 12 (2024): S315. http://dx.doi.org/10.1016/j.jid.2024.10.515.

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Gherardini, J., A. Akhundlu, M. Gompels, et al. "805 Topical thyroid hormones promote hair follicle and epithelial hair follicle stem cell growth in intact human scalp skin ex vivo." Journal of Investigative Dermatology 144, no. 8 (2024): S140. http://dx.doi.org/10.1016/j.jid.2024.06.821.

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D, Prasanthi, Thapaswi I, Vasavi D, and Lakshmi PK. "Dutasteride Topical Gel Containing Herbal Extract for Male Pattern Baldness." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 14, no. 04 (2024): 978–83. https://doi.org/10.25258/ijddt.14.4.8.

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Topical preparations are utilized to deliver drugs directly to the site of application, ensuring localized effects by penetrating into underlying layers of the skin or mucous membrane, thereby offering primary advantage of avoidance of first-pass metabolism. Dutasteride is mainly used for treating benign prostatic hyperplasia (BPH). The research aimed to develop a topical preparation using dutasteride for treating androgenic alopecia (male pattern baldness) wherein incorporation of onion or garlic extract was explored due to their known benefits in promoting hair growth. Various concentrations of gelling agents were preliminary screened for gel consistency. Here, Carbopol-934 was used as gelling agent, for ease of application and aloe vera as an emollient, which gives lustre to hair. The formulations were evaluated for physico-chemical properties, in-vitro diffusion studies, ex-vivo permeation studies and stability giving desirable results. The preparations were highly productive towards nourishing hair roots and producing faster hair growth. A comparative study of the evaluation parameters for different dutasteride gels formulation demonstrated that the garlic-induced preparation (i.e., dutasteride gel with 10% garlic extract) produced more beneficial effect compared to other preparations, thus this prepared formulation can be suggested for male pattern baldness treatment.

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Agramunt, J., J. Cheret, J. Gherardini, F. Jiménez, and R. Paus. "LB1030 Chemosensory biology of human hair follicles: Olfactory receptor OR10J1 stimulation promotes scalp hair follicle growth and progenitor cell generation ex vivo." Journal of Investigative Dermatology 144, no. 8 (2024): S183. http://dx.doi.org/10.1016/j.jid.2024.06.1198.

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Fostier, W., K. Linowiecka, J. Cheret, N. Rajan, and R. Paus. "239 The Birt-Hogg-Dubé syndrome gene folliculin (FLCN) negatively regulates human hair follicle growth ex vivo." Journal of Investigative Dermatology 144, no. 12 (2024): S269. http://dx.doi.org/10.1016/j.jid.2024.10.247.

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Jo, HyungWoo, Seon Yu Kim, Byung Ha Kang, et al. "Staphylococcus epidermidis Cicaria, a Novel Strain Derived from the Human Microbiome, and Its Efficacy as a Treatment for Hair Loss." Molecules 27, no. 16 (2022): 5136. http://dx.doi.org/10.3390/molecules27165136.

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The skin tissue of the scalp is unique from other skin tissues because it coexists with hair, and many differences in microbial composition have been confirmed. In scalp tissues, hair loss occurs due to a combination of internal and external factors, and several studies are being conducted to counteract this. However, not many studies have addressed hair loss from the perspective of the microbiome. In this study, subjects with hair loss and those with normal scalps were set as experimental and control groups, respectively. In the experimental group, hair loss had progressed, and there was a large difference in microbiome composition compared to the group with normal scalps. In particular, differences in Accumulibacter, Staphylococcus, and Corynebacterium were found. From Staphylococcus epidermidis Cicaria, two active components were isolated as a result of repeated column chromatography. Spectroscopic data led to the determination of chemical structures for adenosine and biotin. Fractions were obtained, and ex vivo tests were conducted using hair follicles derived from human scalp tissue. When the microbiome adenosine-treated group was compared to the control group, hair follicle length was increased, and hair root diameter was maintained during the experimental periods. In addition, the Cicaria culture medium and the microbial adenosine- and biotin-treated groups maintained the anagen phase, reducing progression to the catagen phase in the hair growth cycle. In conclusion, it was confirmed that the Cicaria culture medium and the microbial adenosine and biotin derived from the culture were effective in inhibiting hair loss.

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Gherardini, J., T. Rouille, M. Ferholz та ін. "571 Human Hair Follicles can “Taste”: Stimulation of the Bitter Taste Receptor TAS2R4 Inhibits Hair Growth Ex Vivo by Up-Regulating TGF-β2". Journal of Investigative Dermatology 142, № 12 (2022): S279. http://dx.doi.org/10.1016/j.jid.2022.09.587.

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Piccini, Ilaria, Marta Sousa, Sabrina Altendorf, et al. "Intermediate Hair Follicles from Patients with Female Pattern Hair Loss Are Associated with Nutrient Insufficiency and a Quiescent Metabolic Phenotype." Nutrients 14, no. 16 (2022): 3357. http://dx.doi.org/10.3390/nu14163357.

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Female pattern hair loss (FPHL) is a non-scarring alopecia resulting from the progressive conversion of the terminal (t) scalp hair follicles (HFs) into intermediate/miniaturized (i/m) HFs. Although data supporting nutrient deficiency in FPHL HFs are lacking, therapeutic strategies are often associated with nutritional supplementation. Here, we show by metabolic analysis that selected nutrients important for hair growth such as essential amino acids and vitamins are indeed decreased in affected iHFs compared to tHFs in FPHL scalp skin, confirming nutrient insufficiency. iHFs also displayed a more quiescent metabolic phenotype, as indicated by altered metabolite abundance in freshly collected HFs and release/consumption during organ culture of products/substrates of TCA cycle, aerobic glycolysis, and glutaminolysis. Yet, as assessed by exogenous nutrient supplementation ex vivo, nutrient uptake mechanisms are not impaired in affected FPHL iHFs. Moreover, blood vessel density is not diminished in iHFs versus tHFs, despite differences in tHFs from different FPHL scalp locations or versus healthy scalp or changes in the expression of angiogenesis-associated growth factors. Thus, our data reveal that affected iHFs in FPHL display a relative nutrient insufficiency and dormant metabolism, but are still capable of absorbing nutrients, supporting the potential of nutritional supplementation as an adjunct therapy for FPHL.

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Somsukskul, Isariya. "Effect of Orthosiphon stamineus plant extract on in vitro dermal papilla cell proliferation and ex vivo hair growth." Chulalongkorn Medical Journal 61, no. 1 (2017): 41–50. http://dx.doi.org/10.58837/chula.cmj.61.1.5.

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Pye, Derek, Rachel Scholey, Sin Ung, Madoc Dawson, Asim Shahmalak, and Talveen S. Purba. "Activation of the integrated stress response in human hair follicles." PLOS ONE 19, no. 6 (2024): e0303742. http://dx.doi.org/10.1371/journal.pone.0303742.

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Unravelling how energy metabolism and stress responses are regulated in human scalp hair follicles could reveal novel insights into the controls of hair growth and provide new targets to manage hair loss disorders. The Mitochondrial Pyruvate Carrier (MPC) imports pyruvate, produced via glycolysis, into the mitochondria, fuelling the TCA cycle. Previous work has shown that MPC inhibition promotes lactate generation, which activates murine epithelial hair follicle stem cells (eHFSCs). However, by pharmacologically targeting the MPC in short-term human hair follicle ex vivo organ culture experiments using UK-5099, we induced metabolic stress-responsive proliferative arrest throughout the human hair follicle epithelium, including within Keratin 15+ eHFSCs. Through transcriptomics, MPC inhibition was shown to promote a gene expression signature indicative of disrupted FGF, IGF, TGFβ and WNT signalling, mitochondrial dysfunction, and activation of the integrated stress response (ISR), which can arrest cell cycle progression. The ISR, mediated by the transcription factor ATF4, is activated by stressors including amino acid deprivation and ER stress, consistent with MPC inhibition within our model. Using RNAScope, we confirmed the upregulation of both ATF4 and the highly upregulated ATF4-target gene ADM2 on human hair follicle tissue sections in situ. Moreover, treatment with the ISR inhibitor ISRIB attenuated both the upregulation of ADM2 and the proliferative block imposed via MPC inhibition. Together, this work reveals how the human hair follicle, as a complex and metabolically active human tissue system, can dynamically adapt to metabolic stress.

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Prabahar, Kousalya, Ubaidulla Uthumansha, Nehal Elsherbiny та Mona Qushawy. "Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia". Pharmaceuticals 16, № 4 (2023): 563. http://dx.doi.org/10.3390/ph16040563.

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The use of synthetic medication for treating alopecia is restricted because of systemic exposure and related negative effects. Beta-sitosterol (β-ST), a natural chemical, has lately been studied for its potential to promote hair development. The cubosomes with dissolving microneedles (CUBs-MND) created in this study may be a useful starting point for the creation of a sophisticated dermal delivery system for β-ST. Cubosomes (CUBs) were prepared by the emulsification method, using glyceryl monooleate (GMO) as a lipid polymer. CUBs were loaded with dissolving microneedles (MND) fabricated with HA and a PVP-K90 matrix. An ex vivo skin permeation study and an in vivo hair growth efficacy test of β-ST were performed with both CUB and CUB-MND. The average particle size of the CUBs was determined to be 173.67 ± 0.52 nm, with a low polydispersity index (0.3) and a high zeta potential value that prevents the aggregate formation of dispersed particles. When compared to CUBs alone, CUBs-MND displayed higher permeating levels of β-ST at all-time points. In the animals from the CUB-MND group, significant hair development was observed. According to the results of the current investigation, CUBs that integrate dissolving microneedles of β-ST are superior in terms of transdermal skin penetration and activity for the treatment of alopecia.

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Martínez-Pascual, María Antonia, Silvia Sacristán, Elena Toledano-Macías, Pablo Naranjo, and María Luisa Hernández-Bule. "Effects of RF Electric Currents on Hair Follicle Growth and Differentiation: A Possible Treatment for Alopecia." International Journal of Molecular Sciences 25, no. 14 (2024): 7865. http://dx.doi.org/10.3390/ijms25147865.

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Androgenic alopecia (AGA) is the most common type of alopecia and its treatments involve drugs that have various adverse effects and are not completely effective. Radiofrequency-based therapies (RF) are an alternative for AGA treatment. Although there is increasing clinical evidence of the effectiveness of RF for alopecia, its effects at the tissue and cellular level have not been studied in detail. The objective of this study was to analyze ex vivo the potential effect of RF currents used in capacitive resistive electrical transfer (CRET) therapy on AGA. Hair follicles (HFs) were donated by patients with AGA and treated with CRET. AGA-HFs were exposed in vitro to intermittent 448 kHz electric current in subthermal conditions. Cell proliferation (Ki67), apoptosis (TUNEL assay), differentiation (β-catenin), integrity (collagen and MMP9), thickness of the epidermis surrounding HF, proportion of bulge cells and melanoblasts in AGA-HF were analyzed by immunohistochemistry. CRET increased proliferation and decreased death of different populations of AGA-HF cells. In addition, the melanoblasts increased in bulge and the epidermis surrounding the hair follicle thickened. These results support the effectiveness of RF-based therapies for the treatment of alopecia. However, clinical trials are necessary to know the true effectiveness of CRET therapy and other RF therapies for AGA treatment.

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Le Riche, A., J. Nienhaus, H. Erdmann, et al. "LB1010 Ashwagandha-derived exosome-like nanoparticles significantly promote human hair growth ex vivo and in vivo by stimulating the production of the anagen promoting growth factor VEGF-A." Journal of Investigative Dermatology 144, no. 8 (2024): S178. http://dx.doi.org/10.1016/j.jid.2024.06.1267.

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Ho, Fritz Ka-Ho, Albert Bolhuis, and M. Begoña Delgado-Charro. "Prevention and Treatment of Fungal Skin Infections Using Cationic Polymeric Films." Pharmaceutics 13, no. 8 (2021): 1161. http://dx.doi.org/10.3390/pharmaceutics13081161.

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Dermatophytosis is a fungal infection of skin, nails and hair. Treatments can be long and infections are often recurrent, and novel treatments are desirable. Here we tested the use of polymeric films that can be sprayed on the skin for the prevention and treatment of dermatophytosis. The two polymers selected were ABIL T Quat 60 and Eudragit E100, which were tested ex vivo using a porcine skin model, and in vitro using microbiological and microscopy techniques. Acceptability of the polymeric films was tested on the skin of healthy volunteers. The results showed that ABIL and Eudragit films prevented and treated fungal skin infections. Whilst polymer films may provide a physical barrier that prevents fungal colonization, it was shown that both polymers are active antifungals ex vivo and in vitro and have intrinsic antifungal activity. For ABIL, we also established that this polymer binds essential nutrients such as metal ions and sugars, thereby restricting the growth of fungi. When applied to healthy subjects’ skin, the polymeric films neither modified the skin color nor increased trans-epidermal water loss, suggesting a low potential for skin irritation, and the approach was generally found to be acceptable for use by the volunteers. In conclusion, we developed a novel strategy for the potential prevention and treatment of dermatophytosis.

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Piccini, I., M. Fehrholz, O. Egriboz, et al. "020 Sphingosine 1-phosphate receptor signalling promotes hair growth and inhibits perifollicular T-cell expansion and immune privilege collapse ex vivo." Journal of Investigative Dermatology 141, no. 10 (2021): S152. http://dx.doi.org/10.1016/j.jid.2021.08.021.

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Tsalgatidou, Polina C., Eirini-Evangelia Thomloudi, Kallimachos Nifakos, Costas Delis, Anastasia Venieraki, and Panagiotis Katinakis. "Calendula officinalis—A Great Source of Plant Growth Promoting Endophytic Bacteria (PGPEB) and Biological Control Agents (BCA)." Microorganisms 11, no. 1 (2023): 206. http://dx.doi.org/10.3390/microorganisms11010206.

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The application of beneficial bacteria may present an alternative approach to chemical plant protection and fertilization products as they enhance growth and resistance to biotic and abiotic stresses. Plant growth-promoting bacteria are found in the rhizosphere, epiphytically or endophytically (Plant Growth Promoting Endophytic Bacteria, PGPEB). In the present study, 36 out of 119 isolated endophytic bacterial strains from roots, leaves and flowers of the pharmaceutical plant Calendula officinalis were further identified and classified into Bacillus, Pseudomonas, Pantoea, Stenotrophomonas and Rhizobium genera. Selected endophytes were evaluated depending on positive reaction to different plant growth promoting (PGP) traits, motility, survival rate and inhibition of phytopathogenic fungi in vitro and ex vivo (tomato fruit). Bacteria were further assessed for their plant growth effect on Arabidopsis thaliana seedlings and on seed bio-primed tomato plantlets, in vitro. Our results indicated that many bacterial endophytes increased seed germination, promoted plant growth and changed root structure by increasing lateral root density and length and root hair formation. The most promising antagonistic PGPEB strains (Cal.r.29, Cal.l.30, Cal.f.4, Cal.l.11, Cal.f.2.1, Cal.r.19 and Cal.r.11) are indicated as effective biological control agents (BCA) against Botrytis cinerea on detached tomato fruits. Results underlie the utility of beneficial endophytic bacteria for sustainable and efficient crop production and disease control.

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Cohen, Guy, Jeannette Jakus, Sumer Baroud, Raanan Gvirtz, and Sharon Rozenblat. "Development of an Effective Acne Treatment Based on CBD and Herbal Extracts: Preliminary In Vitro, Ex Vivo, and Clinical Evaluation." Evidence-Based Complementary and Alternative Medicine 2023 (April 17, 2023): 1–8. http://dx.doi.org/10.1155/2023/4474255.

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Acne vulgaris, the most common form of acne, is characterized by a mixed eruption of inflammatory and noninflammatory skin lesions primarily affecting the face, upper arms, and trunk. The pathogenesis of acne is multifactorial and includes abnormal keratinization and plugging of the hair follicles, increased sebum production, proliferation and activation of Cutibacterium acnes (C. acnes; formerly Propionibacterium acnes, P. acnes), and finally inflammation. Recent studies have found that cannabidiol (CBD) may be beneficial in the treatment of acne. The aim of this study was to explore natural plant extracts that, when combined with CBD, act synergistically to treat acne by targeting different pathogenic factors while minimizing side effects. The first stage of the study investigated the capacity of different plant extracts and plant extract combinations to reduce C. acnes growth and decrease IL-1β and TNFα secretion from U937 cells. The results found that Centella asiatica triterpene (CAT) extract as well as silymarin (from Silybum marianum fruit extract) had significantly superior anti-inflammatory activity when combined with CBD compared to either ingredient alone. In addition, the CAT extract helped potentiate CBD-induced C. acnes growth inhibition. The three ingredients were integrated into a topical formulation and evaluated in ex vivo human skin organ cultures. The formulation was found to be safe and effective, reducing both IL-6 and IL-8 hypersecretion without hampering epidermal viability. Finally, a preliminary clinical study of this formulation conducted on 30 human subjects showed a statistically significant reduction in acne lesions (mainly inflammatory lesions) and porphyrin levels, thereby establishing a tight correlation between in vitro, ex vivo, and clinical results. Further studies must be conducted to verify the results, including placebo-controlled clinical assessment, to exclude any action of the formulation itself.

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Hsia, Ching-Wu, Ming-Yi Ho, Hao-Ai Shui, Chong-Bin Tsai, and Min-Jen Tseng. "Analysis of Dermal Papilla Cell Interactome Using STRING Database to Profile the ex Vivo Hair Growth Inhibition Effect of a Vinca Alkaloid Drug, Colchicine." International Journal of Molecular Sciences 16, no. 2 (2015): 3579–98. http://dx.doi.org/10.3390/ijms16023579.

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Forraz, Nico, Cécile Bize, Anne-Laure Desroches, et al. "The World’s First Acne Dysbiosis-like Model of Human 3D Ex Vivo Sebaceous Gland Colonized with Cutibacterium acnes and Staphylococcus epidermidis." Microorganisms 11, no. 9 (2023): 2183. http://dx.doi.org/10.3390/microorganisms11092183.

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Acne-prone skin is associated with dysbiosis involving Cutibacterium acnes (C. acnes) and Staphylococcus epidermidis (S. epidermidis) causing increased seborrhea in sebaceous glands (SG) and inflammation. Human primary sebocytes were cultivated using 1.106 UFC/mL C. acnes Type IA (facial acne, ATCC6919) and/or 1.105 UFC/mL S. epidermidis (unknown origin, ATCC12228) for 48 h in our SEB4GLN-optimized media without antibiotics. Bacteria and sebocytes were enumerated and assessed to determine their viability. Lipid production was imaged and quantified via Nile Red staining. SG with hair follicles were microdissected from healthy skin and cultured using 1.105 UFC/mL C. acnes Type 1A and/or 1.104 UFC/mL S. epidermidis (wild-type facial skin strain) through prior fixation and immunostaining for MC5R, C. acnes and nuclei (DAPI) via Z-stack confocal microscopy bioimaging (Leica SP5X & FIJI software, Version 2.9.0). C. acnes growth was not impacted when co-cultivated with sebocytes (2D) or SG (3D) models. Phylotype IA stimulated sebocyte lipid production, which had no impact on viability. The S. epidermidis reference strain overproliferated, inducing sebocyte mortality. For 3D SG model, culture conditions were optimized using a wild-type facial skin strain at a lower concentration, 1:10 ratio to C. acnes, reduced contact time, sequential inoculation and rinsing step. Bioimaging revealed strong C. acnes labeling in the active areas of the pilosebaceous unit. S. epidermidis formed biofilm, which was distributed across the SG via non-specific fluorescence imaging. We developed an innovative model of a sebaceous gland that mimics acne-prone skin with lipid overproduction and virulent phylotype IA C. acnes inoculation.

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Narang, Jasjeet Kaur, Ramandeep Singh Narang, and Mehak . "Formulation and Evaluation of Minoxidil Loaded Submicron Emulsion Based Topical Gel for Treatment of Alopecia." Azerbaijan Pharmaceutical and Pharmacotherapy J 22, no. 2 (2023): 164–73. http://dx.doi.org/10.61336/appj/22-2-35.

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Introduction: Alopecia is a benign hair condition that causes reduced hair growth on the scalp and has afflicted populations worldwide. Aim: The present study is designed to formulate and characterize submicron emulsion-based topical gel containing an anti-hypertensive drug like minoxidil to overcome the adverse effects of conventional oral dosage forms & to provide an improved, targetted therapy for the treatment of alopecia. Methods: The minoxidil-loaded submicron emulsion was prepared by aqueous titration method using a suitable combination of Clove oil (oil phase), Tween 20 (surfactant), and Transcutol P (co-surfactant). Results: The maximum submicron emulsion area obtained after constructing pseudo-ternary phase diagrams was found in S\(_{\text{mix}}\) ratio 4:1. The optimized submicron emulsion formulation (M5) exhibited a pH of 5.4 \(\pm\) 0.17, in vitro release of 95.08 \(\pm\) 0.36%, ex vivo permeation of 79.36 \(\pm\) 0.18%, the particle size of 181.3nm with an uniform particle size distribution \((<1)\) and optimum zeta potential (-8.80 mV). The optimized formulation (M5) was then converted to intogel formulation by adding different concentrations (1%, 1.5%, and 2% w/v) of gelling agents like Carbopol 934. Among them, M5\(_{2 w/v}\) was considered to be the optimized gel formulation based on different evaluation studies. The optimized submicron emulsion-based gel formulation also showed an inherited anti-oxidant potential and remained stable for three months at \(4^{\circ}\). Conclusion: The minoxidil-loaded submicron emulsion-based topical gel formulation could be considered a beneficial nano-approach in contrast to other traditional topical dosage forms for treating alopecia.

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Bajbouj, Khuloud, Zebin Xiao, Leslie A. Hopper, et al. "Abstract 3129: Fibroblast activation protein directed CAR T cells engineered in situ using targeted lipid nanoparticles inhibit progression of pancreatic ductal adenocarcinoma." Cancer Research 85, no. 8_Supplement_1 (2025): 3129. https://doi.org/10.1158/1538-7445.am2025-3129.

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The growth of solid tumors is greatly influenced by stromal components including cancer-associated fibroblasts (CAFs) and desmoplastic matrix through both immune-dependent and immune-independent mechanisms. Fibroblast activation protein (FAP) is a stromal cell target that is selectively expressed at high levels on the cell surface of CAFs. We previously established an ex vivo retroviral-transduced CAR T cell approach to target FAP+ CAFs that resulted in depletion of FAP+ stromal cells, disruption of desmoplastic matrix, enhanced immune infiltration, and inhibition of tumor growth. In this study, we generated FAP-CAR T cells in situ using a novel platform to deliver modified FAP-CAR mRNA encased in anti-CD5 conjugated targeted lipid nanoparticles (tLNPs) and assessed the efficacy of this approach in a preclinical model of pancreatic ductal adenocarcinoma utilizing a cell line derived from a spontaneous pancreatic tumor isolated from a genetically engineered KPC mouse. We found that a single intravenous dose of anti-CD5 tLNP loaded with 30 μg of FAP-CAR mRNA resulted in transient FAP-CAR expression on &gt;45%, &gt;69% and &gt;35% of CD3 T cells in spleen, blood and tumor tissues, respectively. Although CAR expression was more transient, the percentage of FAP-CAR+ T cells generated using this approach was significantly greater than the percentage of FAP-CAR+ cells detected following administration of 1X107 ex vivo retrovirally transduced FAP-CAR T cells. Notably, this in situ mRNA CAR T-cell engineering resulted in significantly increased inhibition of tumor growth, as compared to adoptive transfer of ex vivo retroviral-engineered T cells (74% vs 48%) respectively. Given that this approach circumvents the need for autologous T cell isolation, ex vivo viral transduction and expansion (thereby reducing the potential risk of insertional mutagenesis, while also eliminating the need for pre-conditioning by lymphodepletion), our data indicate a promising therapeutic delivery platform for treating desmoplastic solid tumors using a more accessible and cost-efficient technology. Citation Format: Khuloud Bajbouj, Zebin Xiao, Leslie A. Hopper, Li Huang, Tyler E. Papp, Jayalakshmi Ramani, Adrian Bot, Yanjie Bao, Matthew Butcher, Haig Aghajanian, Carl H. June, Drew Weissman, Hamideh Parhiz, Steven M. Albelda, Ellen Puré. Fibroblast activation protein directed CAR T cells engineered in situ using targeted lipid nanoparticles inhibit progression of pancreatic ductal adenocarcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 3129.

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Philpott, M. P., M. R. Green, and T. Kealey. "Human hair growth in vitro." Journal of Cell Science 97, no. 3 (1990): 463–71. http://dx.doi.org/10.1242/jcs.97.3.463.

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We report for the first time the successful maintenance and growth of human hair follicles in vitro. Human anagen hair follicles were isolated by microdissection from human scalp skin. Isolation of the hair follicles was achieved by cutting the follicle at the dermo-subcutaneous fat interface using a scalpel blade. Intact hair follicles were then removed from the fat using watchmakers' forceps. Isolated hair follicles maintained free-floating in supplemented Williams E medium in individual wells of 24-well multiwell plates showed a significant increase in length over 4 days. The increase in length was seen to be attributed to the production of a keratinised hair shaft, and was not associated with the loss of hair follicle morphology. [methyl-3H]thymidine autoradiography confirmed that in vitro the in vivo pattern of DNA synthesis was maintained; furthermore, [35S]methionine labelling of keratins showed that their patterns of synthesis did not change with maintenance. The importance of this model to hair follicle biology is further demonstrated by the observations that TGF-beta 1 has a negative growth-regulatory effect on hair follicles in vitro and that EGF mimics the in vivo depilatory effects that have been reported in sheep and mice.

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Grewal, Ikmeet Kaur, Sukhbir Singh, Sandeep Arora, Naleem Sharma, Tapan Behl, and Ishrat Zahoor. "Ex-Vivo Evaluation of Eflornithine Hydrochloride Loaded Solid Lipid Microparticles Based Cream." ECS Transactions 107, no. 1 (2022): 8947–51. http://dx.doi.org/10.1149/10701.8947ecst.

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Hirsutism is an unpleasant condition that affects a large number of women. Hirsutism can make females feel exceedingly self-conscious, jeopardizing their sense of femininity as well as their self-esteem. They are employing established treatments without evaluating their negative consequences, necessitating the development of new procedures. EFH loaded solid lipid microparticles (EFH-SLM) were manufactured using the solvent evaporation technique using polymers. Hair regrowth investigations in healthy Swiss albino female mice were carried out ex-vivo. Animals treated with EFH-Cream and EFH-SLM-Cream had the fairest and least dense hair densities on day 21. In comparison to regular EFH-Cream, this revealed that cream loaded with EFH-SLM was more efficient. On day 21, hair weight per cm2 was weighed for all groups of animals, and it was discovered that there was a substantial difference between animals treated with EFH-cream and EFH-SLM-Cream, which could be related to EFH-increased SLM's permeability. In comparison to animals treated with EFH-Cream and marketed EFH cream, animals treated with EFH-SLM-cream had fewer or no skin rashes or inflammation.

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Park, Ki Moon, Dong Woo Kim, and Seung Ho Lee. "Extract ofAllium tuberosum Rottler ex SprengPromoted the Hair Growth through Regulating the Expression of IGF-1." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–11. http://dx.doi.org/10.1155/2015/413538.

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Allium tuberosum Rottler ex Spreng(ATRES) has been used as a traditional medicine for the treatment of abdominal pain, diarrhea, and asthma. In this study, we investigated the hair growth promoting activities of ATRES on telogenic C57BL6/N mice. Hair growth was significantly increased in the dorsal skin of ethanol extract of ATRES treated mouse group compared with the control mouse group. To enrich the hair promoting activity, an ethanol-insoluble fraction was further extracted in sequence withn-hexane, dichloromethane, ethyl acetate,n-butanol, and distilled water. Interestingly, we found that extraction withn-butanol is most efficient in producing the hair promoting activity. In addition, the soluble fraction of then-butanol extract was further separated by silica gel chromatography and thin layer chromatography (TLC) resulting in isolating four single fractions which have hair growth regeneration potential. Furthermore, administration of ATRES extracts to dorsal skin area increased the number of hair follicles compared with control mouse group. Interestingly, administration of ATRES extract stimulated the expression of insulin-like growth factor-1 (IGF-1) but not of keratin growth factor (KGF) or vascular endothelial growth factor (VEGF). Taken together, these results suggest that ATRES possesses strong hair growth promoting potential which controls the expression of IGF-1.

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Mutamimmah, Siti, Elasari Dwi Pratiwi, and Djati Wulan Kusumo. "EFFECT OF HAIR TONIC COMBINATION OF CINNAMOMUN ESSENSIAL OIL AND CANDLENUT ESSENSIAL OIL HAIR GROWTH IN VIVO." Medical Sains : Jurnal Ilmiah Kefarmasian 9, no. 4 (2024): 947–58. http://dx.doi.org/10.37874/ms.v9i4.1610.

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Hair loss, often called alopecia, is characterized by excessive hair loss. One way to treat hair loss is to use hair tonic preparation. This study aimed to determine the effect of a combination of hair tonic cinnamon essential oil and candlenut essential oil on hair growth. The research method used included physical evaluation of the hair tonic preparation formulation by examining the physical quality, homogeneity, pH, viscosity, and stability of the hair tonic preparation. The effectiveness of hair growth was evaluated in vivo in rabbits. Its effectiveness measures hair growth and weight. Based on the results of the tests on the physical characteristics and physical stability of the hair tonic preparation, a combination of cinnamon essential oil and candlenut essential oil at concentrations of 0% (F0), 2.5% (F1), 5% (F2), and 7.5% (F3) was used. in organoleptic testing, the homogeneity, pH, and viscosity met the specified characteristics and stability requirements. The results of research testing the effectiveness of hair growth showed that the hair tonic combination of cinnamon essential oil and candlenut essential oil is effective for good hair growth in formula 3, with a hair length of 8 mm and a hair mass of 213.8 mg on day 21. Keywords: Candlenut essential oil, Cinnamon essential oil, Hair growth, Hair tonic, In vivo

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Charpentier, Emmanuelle, Robert M. Lavker, Elizabeth Acquista, and Pamela Cowin. "Plakoglobin Suppresses Epithelial Proliferation and Hair Growth in Vivo." Journal of Cell Biology 149, no. 2 (2000): 503–20. http://dx.doi.org/10.1083/jcb.149.2.503.

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Plakoglobin regulates cell adhesion by providing a modulatable connection between both classical and desmosomal cadherins and their respective cytoskeletal linker proteins. Both plakoglobin and the related protein β-catenin are posttranscriptionally upregulated in response to Wnt-1 in cultured cells. Upregulation of β-catenin has been implicated in potentiating hyperproliferation and tumor formation. To investigate the role of plakoglobin in these functions we expressed a full-length (PG) and an NH2-terminally truncated form of plakoglobin (ΔN80PG) in mouse epidermis and hair follicles, tissues which undergo continuous and easily observed postnatal renewal and remodeling. Expression of these constructs results in stunted hair growth, a phenotype that has also been observed in transgenic mice expressing Wnt3 and Dvl2 (Millar et al. 1999). Hair follicles from PG and ΔN80PG mice show premature termination of the growth phase (anagen) of the hair cycle, an event that is regulated in part by FGF5 (Hebert et al. 1994). The proliferative rate of the epidermal cells was reduced and apoptotic changes, which are associated with entry into the regressive phase of the hair follicle cycle (catagen), occurred earlier than usual.

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Kovale, Lochana, Seoyeon Lee, Minhyeok Song та ін. "Gynostemma pentaphyllum Hydrodistillate and Its Major Component Damulin B Promote Hair Growth-Inducing Properties In Vivo and In Vitro via the Wnt/β-Catenin Pathway in Dermal Papilla Cells". Nutrients 16, № 7 (2024): 985. http://dx.doi.org/10.3390/nu16070985.

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Alopecia, a prevalent yet challenging condition with limited FDA-approved treatments which is accompanied by notable side effects, necessitates the exploration of natural alternatives. This study elucidated the hair growth properties of Gynostemma pentaphyllum leaf hydrodistillate (GPHD) both in vitro and in vivo. Furthermore, damulin B, a major component of GPHD, demonstrated hair growth-promoting properties in vitro. Beyond its established anti-diabetic, anti-obesity, and anti-inflammatory attributes, GPHD exhibited hair growth induction in mice parallel to minoxidil. Moreover, it upregulated the expression of autocrine factors associated with hair growth, including VEGF, IGF-1, KGF, and HGF. Biochemical assays revealed that minoxidil, GPHD, and damulin B induced hair growth via the Wnt/β-catenin pathway through AKT signaling, aligning with in vivo experiments demonstrating improved expression of growth factors. These findings suggest that GPHD and damulin B contribute to the hair growth-inducing properties of dermal papilla cells through the AKT/β-catenin signaling pathway.

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Semalty, M., A. Semalty, Geeta P. Joshi, and M. S. M. Rawat. "In vivo Hair Growth Activity of Herbal Formulations." International Journal of Pharmacology 6, no. 1 (2009): 53–57. http://dx.doi.org/10.3923/ijp.2010.53.57.

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Kim, Hayeon, Seoungwoo Shin, Youngsu Jang, Eunae Cho, Deokhoon Park, and Eunsun Jung. "Sargassum fusiforme Extract Induces Melanogenesis through the cAMP/PKA/CREB Signaling Pathway." Cosmetics 10, no. 4 (2023): 116. http://dx.doi.org/10.3390/cosmetics10040116.

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The aim of this study was to investigate the effect of Sargassum fusiforme extract (SFE) on melanogenesis and its mechanism both in vitro and ex vivo. The melanogenic-inducing effect of SFE was evaluated using a melanin contents assay and a cellular tyrosinase activity assay. To investigate whether SFE could protect melanocytes against oxidative stress, hydrogen peroxidase was used. The molecular mechanism underlying the effect of SFE on melanogenesis was determined via Western blot analysis of tyrosinase, a microphthalmia-associated transcription factor (MITF), and a phosphorylated cAMP response element-binding protein (p-CREB) expression. The degree of pigmentation in a 3D skin model was determined by measuring the L* values. Contents of melanin in ex vivo human hair follicles were evaluated via Fontana–Masson staining. SFE significantly increased melanin contents and cellular tyrosinase activity in human epidermal melanocytes. SFE also increased the phosphorylation of CREB and the protein levels of tyrosinase and MITF. Moreover, SFE attenuated oxidative stress-induced cytotoxicity and depigmentation. Finally, the melanogenesis promoting effect of SFE was confirmed in both a 3D skin model and ex vivo human hair follicles. These findings suggest that SFE can induce melanogenesis via the cAMP/PKA/CREB signaling pathway in human epidermal melanocytes through its hyperpigmentation activity.

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Journal articles on the topic 'Ex vivo hair growth'

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