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Journal articles on the topic 'Ex vivo hair growth'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Hundt, Jennifer E., Stephanie Sass, Wolfgang Funk, et al. "Mineralocorticoid Receptor Antagonists Stimulate Human Hair Growth ex vivo." Skin Pharmacology and Physiology 32, no. 6 (2019): 344–48. http://dx.doi.org/10.1159/000501729.

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Fernández-Martos, Sandra, María Calvo-Sánchez, Karla García-Alonso, Begoña Castro, Bita Hashtroody, and Jesús Espada. "Sustained Human Hair Follicle Growth Ex Vivo in a Glycosaminoglycan Hydrogel Matrix." International Journal of Molecular Sciences 20, no. 7 (2019): 1741. http://dx.doi.org/10.3390/ijms20071741.

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Glycosaminoglycans (GAGs) and associated proteoglycans have important functions in homeostatic maintenance and regenerative processes (e.g., wound repair) of the skin. However, little is known about the role of these molecules in the regulation of the hair follicle cycle. Here we report that growing human hair follicles ex vivo in a defined GAG hydrogel mimicking the dermal matrix strongly promotes sustained cell survival and maintenance of a highly proliferative phenotype in the hair bulb and suprabulbar regions. This significant effect is associated with the activation of WNT/β-catenin signa

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3

Kwon, Oh Sang, Jun Kyu Oh, Mi Hyang Kim, et al. "Human hair growth ex vivo is correlated with in vivo hair growth: selective categorization of hair follicles for more reliable hair follicle organ culture." Archives of Dermatological Research 297, no. 8 (2005): 367–71. http://dx.doi.org/10.1007/s00403-005-0619-z.

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Haslam, Iain S., Jonathan A. Hardman, and Ralf Paus. "Topically Applied Nicotinamide Inhibits Human Hair Follicle Growth Ex Vivo." Journal of Investigative Dermatology 138, no. 6 (2018): 1420–22. http://dx.doi.org/10.1016/j.jid.2017.12.019.

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5

Bacqueville, Daniel, Marguerite Lévêque, Camille Mas, et al. "New Plant Extracts Exert Complementary Anti‐Hair Loss Properties in Human In Vitro and Ex Vivo Models." Journal of Cosmetic Dermatology 23, S5 (2024): 1–11. http://dx.doi.org/10.1111/jocd.16616.

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ABSTRACTBackgroundHair loss is linked to dysfunction of the growth (anagen), regression (catagen) and rest (telogen) phases of the hair follicle (HF) cycle.AimsTo evaluate the effects of a Silybum marianum extract (SME), manganese PCA (MnPCA), and a Lespedeza capitata extract (LCE) on markers of hair growth and anchorage in human follicle dermal papilla cells (HFDPCs), and to investigate the ability of a topical serum containing these active ingredients to improve HF growth in an ex vivo human scalp skin model.MethodsIn HFDPCs, we assessed receptor tyrosine kinase phosphorylation and Wnt/β‐cat

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Alam, Majid, Diana A. Below, Jérémy Chéret, et al. "Growth Hormone Operates as a Neuroendocrine Regulator of Human Hair Growth Ex Vivo." Journal of Investigative Dermatology 139, no. 7 (2019): 1593–96. http://dx.doi.org/10.1016/j.jid.2018.12.022.

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McElwee, Kevin J., Andrea Huth, Sabine Kissling, and Rolf Hoffmann. "Macrophage-Stimulating Protein Promotes Hair Growth Ex Vivo and Induces Anagen from Telogen Stage Hair Follicles In Vivo." Journal of Investigative Dermatology 123, no. 1 (2004): 34–40. http://dx.doi.org/10.1111/j.0022-202x.2004.22712.x.

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Kim, Jaeyoon, Jae young Shin, Yun-Ho Choi, et al. "Hair Thickness Growth Effect of Adenosine Complex in Male-/Female-Patterned Hair Loss via Inhibition of Androgen Receptor Signaling." International Journal of Molecular Sciences 25, no. 12 (2024): 6534. http://dx.doi.org/10.3390/ijms25126534.

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Aging (senescence) is an unavoidable biological process that results in visible manifestations in all cutaneous tissues, including scalp skin and hair follicles. Previously, we evaluated the molecular function of adenosine in promoting alopecia treatment in vitro. To elucidate the differences in the molecular mechanisms between minoxidil (MNX) and adenosine, gene expression changes in dermal papilla cells were examined. The androgen receptor (AR) pathway was identified as a candidate target of adenosine for hair growth, and the anti-androgenic activity of adenosine was examined in vitro. In ad

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Le Riche, A., J. Nienhaus, M. Sousa, et al. "1420 Ashwagandha-derived exosome-like nanoparticles promote human hair growth ex vivo." Journal of Investigative Dermatology 143, no. 5 (2023): S243. http://dx.doi.org/10.1016/j.jid.2023.03.1436.

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Trivedi, Dahryn, and Snehasis Jana. "In vivo Hair Growth Promotion Efficacy of Biofield Energy Treatment in C57BL/6 Mice." Letters in Health and Biological Sciences 3, no. 2 (2018): 51–55. https://doi.org/10.15436/2475-6245.18.2031.

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Hair loss directly impacts people’s social interactions and also affects psychological well-being. Hair fall has been a rising trend nowadays due to dynamic lifestyle and pollution. In this context,the present study was performed to identify the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (mixture of herbal ex-tracts of Phyllantus emblica and Eclipta alba in 1:1 ratio) on the telogen skin of mice for the assessment of haircell growth and development in vivo. The test item was divided into two parts. One part was denoted as the untreated test item

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Oláh, Attila, Jennifer Gherardini, Marta Bertolini, et al. "The Thyroid Hormone Analogue KB2115 (Eprotirome) Prolongs Human Hair Growth (Anagen) Ex Vivo." Journal of Investigative Dermatology 136, no. 8 (2016): 1711–14. http://dx.doi.org/10.1016/j.jid.2016.03.033.

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Upadhyay, Ratna, Mihir C. Gadani, and Sneha Badak. "Evaluating the hair regenerative potential of plant-based hair serum formulation: a comparative study." International Journal of Scientific Reports 11, no. 1 (2024): 20–24. https://doi.org/10.18203/issn.2454-2156.intjscirep20243799.

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Background: Hair loss, a multifactorial condition influenced by factors such as aging, hormonal imbalances, and genetics, has traditionally been treated with synthetic products like minoxidil and finasteride. However, these treatments often come with significant side effects, highlighting the need for safer and more effective alternatives. This study investigates the efficacy and safety of OZiva+ advanced hair growth serum, a formulation containing 3% Redensyl® and 1% Lindera root extract, as a potential treatment for hair loss. Methods: The serum's hair growth-promoting effects were evaluated

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Fernandes, Bruno, Teresa Matamá, Andreia C. Gomes, and Artur Cavaco-Paulo. "Cyclosporin A-loaded poly(d,l-lactide) nanoparticles: a promising tool for treating alopecia." Nanomedicine 15, no. 15 (2020): 1459–69. http://dx.doi.org/10.2217/nnm-2020-0089.

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Background: Alopecia treatments are scarce and lack efficacy. Cyclosporin A (CsA) has hair growth-inducing properties but its poor cutaneous absorption undermines its use in topical treatments. Aim: Development of a new potential topical treatment of alopecia with CsA. Materials & methods: CsA-loaded poly(d,l-lactide) (PLA) nanoparticles were obtained and characterized. Skin permeation was evaluated in ex vivo porcine skin. Results: Nanoparticles with good physicochemical stability increased CsA skin permeation/hair follicles accumulation, compared with a noncolloidal formulation. CsA bioc

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Choi, Joon Yong, Min Young Boo, and Yong Chool Boo. "Can Plant Extracts Help Prevent Hair Loss or Promote Hair Growth? A Review Comparing Their Therapeutic Efficacies, Phytochemical Components, and Modulatory Targets." Molecules 29, no. 10 (2024): 2288. http://dx.doi.org/10.3390/molecules29102288.

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This narrative review aims to examine the therapeutic potential and mechanism of action of plant extracts in preventing and treating alopecia (baldness). We searched and selected research papers on plant extracts related to hair loss, hair growth, or hair regrowth, and comprehensively compared the therapeutic efficacies, phytochemical components, and modulatory targets of plant extracts. These studies showed that various plant extracts increased the survival and proliferation of dermal papilla cells in vitro, enhanced cell proliferation and hair growth in hair follicles ex vivo, and promoted h

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Gherardini, J., T. Rouille, M. Fehrholz, et al. "743 Hair follicles can “taste”: Stevioside stimulation of the bitter taste receptor, TAS2R4, inhibits human hair growth ex vivo." Journal of Investigative Dermatology 142, no. 8 (2022): S128. http://dx.doi.org/10.1016/j.jid.2022.05.755.

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Suzuki, T., F. Scala, J. Gherardini, et al. "033 IL-15 is an unexpected guardian of hair follicle immune privilege and promotes human hair growth ex vivo." Journal of Investigative Dermatology 141, no. 5 (2021): S6. http://dx.doi.org/10.1016/j.jid.2021.02.049.

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Horesh, Elijah J., Jérémy Chéret, and Ralf Paus. "Growth Hormone and the Human Hair Follicle." International Journal of Molecular Sciences 22, no. 24 (2021): 13205. http://dx.doi.org/10.3390/ijms222413205.

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Ever since the discoveries that human hair follicles (HFs) display the functional peripheral equivalent of the hypothalamic-pituitary-adrenal axis, exhibit elements of the hypothalamic-pituitary-thyroid axis, and even generate melatonin and prolactin, human hair research has proven to be a treasure chest for the exploration of neurohormone functions. However, growth hormone (GH), one of the dominant neurohormones of human neuroendocrine physiology, remains to be fully explored in this context. This is interesting since it has long been appreciated clinically that excessive GH serum levels indu

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18

Rajendran, Ramya Lakshmi, Prakash Gangadaran, Mi Hee Kwack, et al. "Engineered Nanovesicles from Fibroblasts Modulate Dermal Papillae Cells In Vitro and Promote Human Hair Follicle Growth Ex Vivo." Cells 11, no. 24 (2022): 4066. http://dx.doi.org/10.3390/cells11244066.

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Alopecia is a common medical condition affecting both sexes. Dermal papilla (DP) cells are the primary source of hair regeneration in alopecia patients. Therapeutic applications of extracellular vesicles (EVs) are restricted by low yields, high costs, and their time-consuming collection process. Thus, engineered nanovesicles (eNVs) have emerged as suitable therapeutic biomaterials in translational medicine. We isolated eNVs by the serial extrusion of fibroblasts (FBs) using polycarbonate membrane filters and serial and ultracentrifugation. We studied the internalization, proliferation, and mig

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Lim, Hye Won, Hak Joong Kim, Chae Young Jeon, et al. "Hair Growth Promoting Effects of 15-Hydroxyprostaglandin Dehydrogenase Inhibitor in Human Follicle Dermal Papilla Cells." International Journal of Molecular Sciences 25, no. 13 (2024): 7485. http://dx.doi.org/10.3390/ijms25137485.

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Prostaglandin E2 (PGE2) is known to be effective in regenerating tissues, and bimatoprost, an analog of PGF2α, has been approved by the FDA as an eyelash growth promoter and has been proven effective in human hair follicles. Thus, to enhance PGE2 levels while improving hair loss, we found dihydroisoquinolinone piperidinylcarboxy pyrazolopyridine (DPP), an inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), using DeepZema®, an AI-based drug development program. Here, we investigated whether DPP improved hair loss in human follicle dermal papilla cells (HFDPCs) damaged by dihydrotestos

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Trivedi, Mahendra Kumar, and Snehasis Jana. "Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture." JOURNAL OF DERMATOLOGICAL RESEARCH AND THERAPY 1, no. 3 (2019): 12–19. https://doi.org/10.14302/issn.2471-2175.jdrt-18-2520.

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Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as th

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Bassino, Eleonora, Franco Gasparri, and Luca Munaron. "Protective Role of Nutritional Plants Containing Flavonoids in Hair Follicle Disruption: A Review." International Journal of Molecular Sciences 21, no. 2 (2020): 523. http://dx.doi.org/10.3390/ijms21020523.

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Hair loss is a disorder in which the hair falls out from skin areas such as the scalp and the body. Several studies suggest the use of herbal medicine to treat related disorders, including alopecia. Dermal microcirculation is essential for hair maintenance, and an insufficient blood supply can lead to hair follicles (HF) diseases. This work aims to provide an insight into the ethnohistorical records of some nutritional compounds containing flavonoids for their potential beneficial features in repairing or recovering from hair follicle disruption. We started from a query for “alopecia” OR “hair

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22

Agramunt, J., J. Cheret, J. Gherardini, F. Jimenez, and R. Paus. "503 A new chemosensory control of human hair growth: Olfactory receptor OR10J1 stimulation promotes scalp hair follicle growth and progenitor cell generation ex vivo." Journal of Investigative Dermatology 144, no. 12 (2024): S315. http://dx.doi.org/10.1016/j.jid.2024.10.515.

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23

Gherardini, J., A. Akhundlu, M. Gompels, et al. "805 Topical thyroid hormones promote hair follicle and epithelial hair follicle stem cell growth in intact human scalp skin ex vivo." Journal of Investigative Dermatology 144, no. 8 (2024): S140. http://dx.doi.org/10.1016/j.jid.2024.06.821.

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D, Prasanthi, Thapaswi I, Vasavi D, and Lakshmi PK. "Dutasteride Topical Gel Containing Herbal Extract for Male Pattern Baldness." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 14, no. 04 (2024): 978–83. https://doi.org/10.25258/ijddt.14.4.8.

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Topical preparations are utilized to deliver drugs directly to the site of application, ensuring localized effects by penetrating into underlying layers of the skin or mucous membrane, thereby offering primary advantage of avoidance of first-pass metabolism. Dutasteride is mainly used for treating benign prostatic hyperplasia (BPH). The research aimed to develop a topical preparation using dutasteride for treating androgenic alopecia (male pattern baldness) wherein incorporation of onion or garlic extract was explored due to their known benefits in promoting hair growth. Various concentrations

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Agramunt, J., J. Cheret, J. Gherardini, F. Jiménez, and R. Paus. "LB1030 Chemosensory biology of human hair follicles: Olfactory receptor OR10J1 stimulation promotes scalp hair follicle growth and progenitor cell generation ex vivo." Journal of Investigative Dermatology 144, no. 8 (2024): S183. http://dx.doi.org/10.1016/j.jid.2024.06.1198.

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Fostier, W., K. Linowiecka, J. Cheret, N. Rajan, and R. Paus. "239 The Birt-Hogg-Dubé syndrome gene folliculin (FLCN) negatively regulates human hair follicle growth ex vivo." Journal of Investigative Dermatology 144, no. 12 (2024): S269. http://dx.doi.org/10.1016/j.jid.2024.10.247.

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Jo, HyungWoo, Seon Yu Kim, Byung Ha Kang, et al. "Staphylococcus epidermidis Cicaria, a Novel Strain Derived from the Human Microbiome, and Its Efficacy as a Treatment for Hair Loss." Molecules 27, no. 16 (2022): 5136. http://dx.doi.org/10.3390/molecules27165136.

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The skin tissue of the scalp is unique from other skin tissues because it coexists with hair, and many differences in microbial composition have been confirmed. In scalp tissues, hair loss occurs due to a combination of internal and external factors, and several studies are being conducted to counteract this. However, not many studies have addressed hair loss from the perspective of the microbiome. In this study, subjects with hair loss and those with normal scalps were set as experimental and control groups, respectively. In the experimental group, hair loss had progressed, and there was a la

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Gherardini, J., T. Rouille, M. Ferholz та ін. "571 Human Hair Follicles can “Taste”: Stimulation of the Bitter Taste Receptor TAS2R4 Inhibits Hair Growth Ex Vivo by Up-Regulating TGF-β2". Journal of Investigative Dermatology 142, № 12 (2022): S279. http://dx.doi.org/10.1016/j.jid.2022.09.587.

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Piccini, Ilaria, Marta Sousa, Sabrina Altendorf, et al. "Intermediate Hair Follicles from Patients with Female Pattern Hair Loss Are Associated with Nutrient Insufficiency and a Quiescent Metabolic Phenotype." Nutrients 14, no. 16 (2022): 3357. http://dx.doi.org/10.3390/nu14163357.

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Female pattern hair loss (FPHL) is a non-scarring alopecia resulting from the progressive conversion of the terminal (t) scalp hair follicles (HFs) into intermediate/miniaturized (i/m) HFs. Although data supporting nutrient deficiency in FPHL HFs are lacking, therapeutic strategies are often associated with nutritional supplementation. Here, we show by metabolic analysis that selected nutrients important for hair growth such as essential amino acids and vitamins are indeed decreased in affected iHFs compared to tHFs in FPHL scalp skin, confirming nutrient insufficiency. iHFs also displayed a m

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Somsukskul, Isariya. "Effect of Orthosiphon stamineus plant extract on in vitro dermal papilla cell proliferation and ex vivo hair growth." Chulalongkorn Medical Journal 61, no. 1 (2017): 41–50. http://dx.doi.org/10.58837/chula.cmj.61.1.5.

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Pye, Derek, Rachel Scholey, Sin Ung, Madoc Dawson, Asim Shahmalak, and Talveen S. Purba. "Activation of the integrated stress response in human hair follicles." PLOS ONE 19, no. 6 (2024): e0303742. http://dx.doi.org/10.1371/journal.pone.0303742.

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Unravelling how energy metabolism and stress responses are regulated in human scalp hair follicles could reveal novel insights into the controls of hair growth and provide new targets to manage hair loss disorders. The Mitochondrial Pyruvate Carrier (MPC) imports pyruvate, produced via glycolysis, into the mitochondria, fuelling the TCA cycle. Previous work has shown that MPC inhibition promotes lactate generation, which activates murine epithelial hair follicle stem cells (eHFSCs). However, by pharmacologically targeting the MPC in short-term human hair follicle ex vivo organ culture experime

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Prabahar, Kousalya, Ubaidulla Uthumansha, Nehal Elsherbiny та Mona Qushawy. "Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia". Pharmaceuticals 16, № 4 (2023): 563. http://dx.doi.org/10.3390/ph16040563.

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The use of synthetic medication for treating alopecia is restricted because of systemic exposure and related negative effects. Beta-sitosterol (β-ST), a natural chemical, has lately been studied for its potential to promote hair development. The cubosomes with dissolving microneedles (CUBs-MND) created in this study may be a useful starting point for the creation of a sophisticated dermal delivery system for β-ST. Cubosomes (CUBs) were prepared by the emulsification method, using glyceryl monooleate (GMO) as a lipid polymer. CUBs were loaded with dissolving microneedles (MND) fabricated with H

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Martínez-Pascual, María Antonia, Silvia Sacristán, Elena Toledano-Macías, Pablo Naranjo, and María Luisa Hernández-Bule. "Effects of RF Electric Currents on Hair Follicle Growth and Differentiation: A Possible Treatment for Alopecia." International Journal of Molecular Sciences 25, no. 14 (2024): 7865. http://dx.doi.org/10.3390/ijms25147865.

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Androgenic alopecia (AGA) is the most common type of alopecia and its treatments involve drugs that have various adverse effects and are not completely effective. Radiofrequency-based therapies (RF) are an alternative for AGA treatment. Although there is increasing clinical evidence of the effectiveness of RF for alopecia, its effects at the tissue and cellular level have not been studied in detail. The objective of this study was to analyze ex vivo the potential effect of RF currents used in capacitive resistive electrical transfer (CRET) therapy on AGA. Hair follicles (HFs) were donated by p

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Le Riche, A., J. Nienhaus, H. Erdmann, et al. "LB1010 Ashwagandha-derived exosome-like nanoparticles significantly promote human hair growth ex vivo and in vivo by stimulating the production of the anagen promoting growth factor VEGF-A." Journal of Investigative Dermatology 144, no. 8 (2024): S178. http://dx.doi.org/10.1016/j.jid.2024.06.1267.

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Ho, Fritz Ka-Ho, Albert Bolhuis, and M. Begoña Delgado-Charro. "Prevention and Treatment of Fungal Skin Infections Using Cationic Polymeric Films." Pharmaceutics 13, no. 8 (2021): 1161. http://dx.doi.org/10.3390/pharmaceutics13081161.

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Dermatophytosis is a fungal infection of skin, nails and hair. Treatments can be long and infections are often recurrent, and novel treatments are desirable. Here we tested the use of polymeric films that can be sprayed on the skin for the prevention and treatment of dermatophytosis. The two polymers selected were ABIL T Quat 60 and Eudragit E100, which were tested ex vivo using a porcine skin model, and in vitro using microbiological and microscopy techniques. Acceptability of the polymeric films was tested on the skin of healthy volunteers. The results showed that ABIL and Eudragit films pre

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Piccini, I., M. Fehrholz, O. Egriboz, et al. "020 Sphingosine 1-phosphate receptor signalling promotes hair growth and inhibits perifollicular T-cell expansion and immune privilege collapse ex vivo." Journal of Investigative Dermatology 141, no. 10 (2021): S152. http://dx.doi.org/10.1016/j.jid.2021.08.021.

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Tsalgatidou, Polina C., Eirini-Evangelia Thomloudi, Kallimachos Nifakos, Costas Delis, Anastasia Venieraki, and Panagiotis Katinakis. "Calendula officinalis—A Great Source of Plant Growth Promoting Endophytic Bacteria (PGPEB) and Biological Control Agents (BCA)." Microorganisms 11, no. 1 (2023): 206. http://dx.doi.org/10.3390/microorganisms11010206.

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The application of beneficial bacteria may present an alternative approach to chemical plant protection and fertilization products as they enhance growth and resistance to biotic and abiotic stresses. Plant growth-promoting bacteria are found in the rhizosphere, epiphytically or endophytically (Plant Growth Promoting Endophytic Bacteria, PGPEB). In the present study, 36 out of 119 isolated endophytic bacterial strains from roots, leaves and flowers of the pharmaceutical plant Calendula officinalis were further identified and classified into Bacillus, Pseudomonas, Pantoea, Stenotrophomonas and

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Cohen, Guy, Jeannette Jakus, Sumer Baroud, Raanan Gvirtz, and Sharon Rozenblat. "Development of an Effective Acne Treatment Based on CBD and Herbal Extracts: Preliminary In Vitro, Ex Vivo, and Clinical Evaluation." Evidence-Based Complementary and Alternative Medicine 2023 (April 17, 2023): 1–8. http://dx.doi.org/10.1155/2023/4474255.

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Acne vulgaris, the most common form of acne, is characterized by a mixed eruption of inflammatory and noninflammatory skin lesions primarily affecting the face, upper arms, and trunk. The pathogenesis of acne is multifactorial and includes abnormal keratinization and plugging of the hair follicles, increased sebum production, proliferation and activation of Cutibacterium acnes (C. acnes; formerly Propionibacterium acnes, P. acnes), and finally inflammation. Recent studies have found that cannabidiol (CBD) may be beneficial in the treatment of acne. The aim of this study was to explore natural

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Hsia, Ching-Wu, Ming-Yi Ho, Hao-Ai Shui, Chong-Bin Tsai, and Min-Jen Tseng. "Analysis of Dermal Papilla Cell Interactome Using STRING Database to Profile the ex Vivo Hair Growth Inhibition Effect of a Vinca Alkaloid Drug, Colchicine." International Journal of Molecular Sciences 16, no. 2 (2015): 3579–98. http://dx.doi.org/10.3390/ijms16023579.

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Broadley, D., A. Le Riche, Y. Yu, et al. "0953 Double knockdown of DKK1 and SFRP1, two key players in androgenetic alopecia, does not accelerate the hair-growth promoting effect of individual SFRP1 knockdown in healthy human hair follicles ex vivo." Journal of Investigative Dermatology 145, no. 8 (2025): S165. https://doi.org/10.1016/j.jid.2025.06.969.

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Forraz, Nico, Cécile Bize, Anne-Laure Desroches, et al. "The World’s First Acne Dysbiosis-like Model of Human 3D Ex Vivo Sebaceous Gland Colonized with Cutibacterium acnes and Staphylococcus epidermidis." Microorganisms 11, no. 9 (2023): 2183. http://dx.doi.org/10.3390/microorganisms11092183.

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Acne-prone skin is associated with dysbiosis involving Cutibacterium acnes (C. acnes) and Staphylococcus epidermidis (S. epidermidis) causing increased seborrhea in sebaceous glands (SG) and inflammation. Human primary sebocytes were cultivated using 1.106 UFC/mL C. acnes Type IA (facial acne, ATCC6919) and/or 1.105 UFC/mL S. epidermidis (unknown origin, ATCC12228) for 48 h in our SEB4GLN-optimized media without antibiotics. Bacteria and sebocytes were enumerated and assessed to determine their viability. Lipid production was imaged and quantified via Nile Red staining. SG with hair follicles

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Narang, Jasjeet Kaur, Ramandeep Singh Narang, and Mehak . "Formulation and Evaluation of Minoxidil Loaded Submicron Emulsion Based Topical Gel for Treatment of Alopecia." Azerbaijan Pharmaceutical and Pharmacotherapy J 22, no. 2 (2023): 164–73. http://dx.doi.org/10.61336/appj/22-2-35.

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Introduction: Alopecia is a benign hair condition that causes reduced hair growth on the scalp and has afflicted populations worldwide. Aim: The present study is designed to formulate and characterize submicron emulsion-based topical gel containing an anti-hypertensive drug like minoxidil to overcome the adverse effects of conventional oral dosage forms & to provide an improved, targetted therapy for the treatment of alopecia. Methods: The minoxidil-loaded submicron emulsion was prepared by aqueous titration method using a suitable combination of Clove oil (oil phase), Tween 20 (surfactant

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Bajbouj, Khuloud, Zebin Xiao, Leslie A. Hopper, et al. "Abstract 3129: Fibroblast activation protein directed CAR T cells engineered in situ using targeted lipid nanoparticles inhibit progression of pancreatic ductal adenocarcinoma." Cancer Research 85, no. 8_Supplement_1 (2025): 3129. https://doi.org/10.1158/1538-7445.am2025-3129.

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The growth of solid tumors is greatly influenced by stromal components including cancer-associated fibroblasts (CAFs) and desmoplastic matrix through both immune-dependent and immune-independent mechanisms. Fibroblast activation protein (FAP) is a stromal cell target that is selectively expressed at high levels on the cell surface of CAFs. We previously established an ex vivo retroviral-transduced CAR T cell approach to target FAP+ CAFs that resulted in depletion of FAP+ stromal cells, disruption of desmoplastic matrix, enhanced immune infiltration, and inhibition of tumor growth. In this stud

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Philpott, M. P., M. R. Green, and T. Kealey. "Human hair growth in vitro." Journal of Cell Science 97, no. 3 (1990): 463–71. http://dx.doi.org/10.1242/jcs.97.3.463.

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We report for the first time the successful maintenance and growth of human hair follicles in vitro. Human anagen hair follicles were isolated by microdissection from human scalp skin. Isolation of the hair follicles was achieved by cutting the follicle at the dermo-subcutaneous fat interface using a scalpel blade. Intact hair follicles were then removed from the fat using watchmakers' forceps. Isolated hair follicles maintained free-floating in supplemented Williams E medium in individual wells of 24-well multiwell plates showed a significant increase in length over 4 days. The increase in le

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Grewal, Ikmeet Kaur, Sukhbir Singh, Sandeep Arora, Naleem Sharma, Tapan Behl, and Ishrat Zahoor. "Ex-Vivo Evaluation of Eflornithine Hydrochloride Loaded Solid Lipid Microparticles Based Cream." ECS Transactions 107, no. 1 (2022): 8947–51. http://dx.doi.org/10.1149/10701.8947ecst.

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Hirsutism is an unpleasant condition that affects a large number of women. Hirsutism can make females feel exceedingly self-conscious, jeopardizing their sense of femininity as well as their self-esteem. They are employing established treatments without evaluating their negative consequences, necessitating the development of new procedures. EFH loaded solid lipid microparticles (EFH-SLM) were manufactured using the solvent evaporation technique using polymers. Hair regrowth investigations in healthy Swiss albino female mice were carried out ex-vivo. Animals treated with EFH-Cream and EFH-SLM-C

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Park, Ki Moon, Dong Woo Kim, and Seung Ho Lee. "Extract ofAllium tuberosum Rottler ex SprengPromoted the Hair Growth through Regulating the Expression of IGF-1." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–11. http://dx.doi.org/10.1155/2015/413538.

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Allium tuberosum Rottler ex Spreng(ATRES) has been used as a traditional medicine for the treatment of abdominal pain, diarrhea, and asthma. In this study, we investigated the hair growth promoting activities of ATRES on telogenic C57BL6/N mice. Hair growth was significantly increased in the dorsal skin of ethanol extract of ATRES treated mouse group compared with the control mouse group. To enrich the hair promoting activity, an ethanol-insoluble fraction was further extracted in sequence withn-hexane, dichloromethane, ethyl acetate,n-butanol, and distilled water. Interestingly, we found that

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Mutamimmah, Siti, Elasari Dwi Pratiwi, and Djati Wulan Kusumo. "EFFECT OF HAIR TONIC COMBINATION OF CINNAMOMUN ESSENSIAL OIL AND CANDLENUT ESSENSIAL OIL HAIR GROWTH IN VIVO." Medical Sains : Jurnal Ilmiah Kefarmasian 9, no. 4 (2024): 947–58. http://dx.doi.org/10.37874/ms.v9i4.1610.

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Hair loss, often called alopecia, is characterized by excessive hair loss. One way to treat hair loss is to use hair tonic preparation. This study aimed to determine the effect of a combination of hair tonic cinnamon essential oil and candlenut essential oil on hair growth. The research method used included physical evaluation of the hair tonic preparation formulation by examining the physical quality, homogeneity, pH, viscosity, and stability of the hair tonic preparation. The effectiveness of hair growth was evaluated in vivo in rabbits. Its effectiveness measures hair growth and weight. Bas

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Charpentier, Emmanuelle, Robert M. Lavker, Elizabeth Acquista, and Pamela Cowin. "Plakoglobin Suppresses Epithelial Proliferation and Hair Growth in Vivo." Journal of Cell Biology 149, no. 2 (2000): 503–20. http://dx.doi.org/10.1083/jcb.149.2.503.

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Plakoglobin regulates cell adhesion by providing a modulatable connection between both classical and desmosomal cadherins and their respective cytoskeletal linker proteins. Both plakoglobin and the related protein β-catenin are posttranscriptionally upregulated in response to Wnt-1 in cultured cells. Upregulation of β-catenin has been implicated in potentiating hyperproliferation and tumor formation. To investigate the role of plakoglobin in these functions we expressed a full-length (PG) and an NH2-terminally truncated form of plakoglobin (ΔN80PG) in mouse epidermis and hair follicles, tissue

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Kovale, Lochana, Seoyeon Lee, Minhyeok Song та ін. "Gynostemma pentaphyllum Hydrodistillate and Its Major Component Damulin B Promote Hair Growth-Inducing Properties In Vivo and In Vitro via the Wnt/β-Catenin Pathway in Dermal Papilla Cells". Nutrients 16, № 7 (2024): 985. http://dx.doi.org/10.3390/nu16070985.

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Alopecia, a prevalent yet challenging condition with limited FDA-approved treatments which is accompanied by notable side effects, necessitates the exploration of natural alternatives. This study elucidated the hair growth properties of Gynostemma pentaphyllum leaf hydrodistillate (GPHD) both in vitro and in vivo. Furthermore, damulin B, a major component of GPHD, demonstrated hair growth-promoting properties in vitro. Beyond its established anti-diabetic, anti-obesity, and anti-inflammatory attributes, GPHD exhibited hair growth induction in mice parallel to minoxidil. Moreover, it upregulate

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Kim, Hayeon, Seoungwoo Shin, Youngsu Jang, Eunae Cho, Deokhoon Park, and Eunsun Jung. "Sargassum fusiforme Extract Induces Melanogenesis through the cAMP/PKA/CREB Signaling Pathway." Cosmetics 10, no. 4 (2023): 116. http://dx.doi.org/10.3390/cosmetics10040116.

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The aim of this study was to investigate the effect of Sargassum fusiforme extract (SFE) on melanogenesis and its mechanism both in vitro and ex vivo. The melanogenic-inducing effect of SFE was evaluated using a melanin contents assay and a cellular tyrosinase activity assay. To investigate whether SFE could protect melanocytes against oxidative stress, hydrogen peroxidase was used. The molecular mechanism underlying the effect of SFE on melanogenesis was determined via Western blot analysis of tyrosinase, a microphthalmia-associated transcription factor (MITF), and a phosphorylated cAMP respo

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