Relevant bibliographies by topics / Expressed sequence tags (EST)

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Academic literature on the topic 'Expressed sequence tags (EST)'

Author: Grafiati
Published: 3 June 2025
Last updated: 22 June 2025

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Journal articles on the topic "Expressed sequence tags (EST)"

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Györgyey, János, Danièle Vaubert, José I. Jiménez-Zurdo, et al. "Analysis of Medicago truncatula Nodule Expressed Sequence Tags." Molecular Plant-Microbe Interactions® 13, no. 1 (2000): 62–71. http://dx.doi.org/10.1094/mpmi.2000.13.1.62.

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Systematic sequencing of expressed sequence tags (ESTs) can give a global picture of the assembly of genes involved in the development and function of organs. Indeterminate nodules representing different stages of the developmental program are especially suited to the study of organogenesis. With the vector λHybriZAP, a cDNA library was constructed from emerging nodules of Medicago truncatula induced by Sinorhizobium meliloti. The 5′ ends of 389 cDNA clones were sequenced, then these ESTs were analyzed both by sequence homology search and by studying their expression in roots and nodules. Two hundred fifty-six ESTs exhibited significant similarities to characterized data base entries and 40 of them represented 26 nodulin genes, while 133 had no similarity to sequences with known function. Only 60 out of the 389 cDNA clones corresponded to previously submitted M. truncatula EST sequences. For 117 cDNAs, reverse Northern (RNA) hybridization with root and nodule RNA probes revealed enhanced expression in the nodule, 48 clones are likely to code for novel nodulins, 33 cDNAs are clones of already known nodulin genes, and 36 clones exhibit similarity to other characterized genes. Thus, systematic analysis of the EST sequences and their expression patterns is a powerful way to identify nodule-specific and nodulation-related genes.
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Cutri, Lucas, and Marcelo Carnier Dornelas. "PASSIOMA: Exploring Expressed Sequence Tags during Flower Development inPassifloraspp." Comparative and Functional Genomics 2012 (2012): 1–11. http://dx.doi.org/10.1155/2012/510549.

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The genusPassifloraprovides a remarkable example of floral complexity and diversity. The extreme variation ofPassifloraflower morphologies allowed a wide range of interactions with pollinators to evolve. We used the analysis of expressed sequence tags (ESTs) as an approach for the characterization of genes expressed duringPassiflorareproductive development. Analyzing thePassiflorafloral EST database (named PASSIOMA), we found sequences showing significant sequence similarity to genes known to be involved in reproductive development such as MADS-box genes. Some of these sequences were studied using RT-PCR andin situhybridization confirming their expression duringPassifloraflower development. The detection of these novel sequences can contribute to the development of EST-based markers for important agronomic traits as well as to the establishment of genomic tools to study the naturally occurring floral diversity amongPassifloraspecies.
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Ozdemir Ozgenturk, Nehir, Fatma Oruç, Ugur Sezerman, et al. "Generation and Analysis of Expressed Sequence Tags fromOlea europaeaL." Comparative and Functional Genomics 2010 (2010): 1–9. http://dx.doi.org/10.1155/2010/757512.

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Olive (Olea europaeaL.) is an important source of edible oil which was originated in Near-East region. In this study, two cDNA libraries were constructed from young olive leaves and immature olive fruits for generation of ESTs to discover the novel genes and search the function of unknown genes of olive. The randomly selected 3840 colonies were sequenced for EST collection from both libraries. Readable 2228 sequences for olive leaf and 1506 sequences for olive fruit were assembled into 205 and 69 contigs, respectively, whereas 2478 were singletons. Putative functions of all 2752 differentially expressed unique sequences were designated by gene homology based on BLAST and annotated using BLAST2GO. While 1339 ESTs show no homology to the database, 2024 ESTs have homology (under 80%) with hypothetical proteins, putative proteins, expressed proteins, and unknown proteins in NCBI-GenBank. 635 EST's unique genes sequence have been identified by over 80% homology to known function in other species which were not previously described in Olea family. Only 3.1% of total EST's was shown similarity with olive database existing in NCBI. This generated EST's data and consensus sequences were submitted to NCBI as valuable source for functional genome studies of olive.
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Watanabe, Hajime, Norihisa Tatarazako, Shigeto Oda, et al. "Analysis of expressed sequence tags of the water flea Daphnia magna." Genome 48, no. 4 (2005): 606–9. http://dx.doi.org/10.1139/g05-038.

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To study gene expression in the water flea Daphnia magna we constructed a cDNA library and characterized the expressed sequence tags (ESTs) of 7210 clones. The EST sequences clustered into 2958 nonredundant groups. BLAST analyses of both protein and DNA databases showed that 1218 (41%) of the unique sequences shared significant similarities to known nucleotide or amino acid sequences, whereas the remaining 1740 (59%) showed no significant similarities to other genes. Clustering analysis revealed particularly high expression of genes related to ATP synthesis, structural proteins, and proteases. The cDNA clones and EST sequence information should be useful for future functional analysis of daphnid biology and investigation of the links between ecology and genomics.Key words: Daphnia magna, EST, classification, ATP synthesis.
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Bettini, Ezio, Angela R. Porta, Norbert Dahmen, Henry Wang, and Frank L. Margolis. "Expressed sequence tags (EST) identify genes preferentially expressed in catfish chemosensory tissues." Molecular Brain Research 23, no. 4 (1994): 285–91. http://dx.doi.org/10.1016/0169-328x(94)90237-2.

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Levi, Amnon, Angela Davis, Pat Wechter, Alvaro Hernandez, and Jyothi Thimmapuram. "DEVELOPING EXPRESSED SEQUENCED TAGS (ESTS) FOR WATERMELON FRUIT." HortScience 41, no. 3 (2006): 518F—519. http://dx.doi.org/10.21273/hortsci.41.3.518f.

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A cDNA library was assembled using mRNA of watermelon fruit. The cDNA library was normalized and subtracted by hybridization with leaf cDNA of the same watermelon cultivar (Illini Red). 1,046 cDNA clones were sequenced to identify genes associated with fruit development and quality. Of 1,046 cDNA clones sequenced, 832 were unique sequences and designated as expressed sequenced tags (ESTs). Of the 832 ESTs, 205 (24.6%) have not been reported in any other plant species. Additionally, 186 ESTs (22.4%) correspond to genes with unknown function, while 441 ESTs (53.0%) correspond to genes with known function in other plant species. These ESTs are mainly associated with primary metabolism, membrane transport, cytoskeleton synthesis and structure, cell wall and cell division, signal transduction, nucleic acid binding and transcription factors, and defense and stress response. Differential expression of the ESTs was examined using microarray analysis. About 200 (24%) of the 832 ESTs showed differential expression during the development and ripening of watermelon fruit. The ESTs were also screened for simple sequence repeat (SSR) motifs. Of 832 ESTs screened, 177 contain SSR motifs. Primer pairs are being designed for these ESTs, and will be used for development of EST-SSR markers and for mapping on a genetic linkage map constructed for watermelon. This study provides valuable information on genes controlling watermelon fruit development and quality.
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Dyhrman, Sonya T., Sheean T. Haley, Shanda R. Birkeland, Louie L. Wurch, Michael J. Cipriano, and Andrew G. McArthur. "Long Serial Analysis of Gene Expression for Gene Discovery and Transcriptome Profiling in the Widespread Marine Coccolithophore Emiliania huxleyi." Applied and Environmental Microbiology 72, no. 1 (2006): 252–60. http://dx.doi.org/10.1128/aem.72.1.252-260.2006.

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ABSTRACT The abundant and widespread coccolithophore Emiliania huxleyi plays an important role in mediating CO2 exchange between the ocean and the atmosphere through its impact on marine photosynthesis and calcification. Here, we use long serial analysis of gene expression (SAGE) to identify E. huxleyi genes responsive to nitrogen (N) or phosphorus (P) starvation. Long SAGE is an elegant approach for examining quantitative and comprehensive gene expression patterns without a priori knowledge of gene sequences via the detection of 21-bp nucleotide sequence tags. E. huxleyi appears to have a robust transcriptional-level response to macronutrient deficiency, with 42 tags uniquely present or up-regulated twofold or greater in the N-starved library and 128 tags uniquely present or up-regulated twofold or greater in the P-starved library. The expression patterns of several tags were validated with reverse transcriptase PCR. Roughly 48% of these differentially expressed tags could be mapped to publicly available genomic or expressed sequence tag (EST) sequence data. For example, in the P-starved library a number of the tags mapped to genes with a role in P scavenging, including a putative phosphate-repressible permease and a putative polyphosphate synthetase. In short, the long SAGE analyses have (i) identified many new differentially regulated gene sequences, (ii) assigned regulation data to EST sequences with no database homology and unknown function, and (iii) highlighted previously uncharacterized aspects of E. huxleyi N and P physiology. To this end, our long SAGE libraries provide a new public resource for gene discovery and transcriptional analysis in this biogeochemically important marine organism.
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Das, Nabajit, Saswat Suvesh Rout, and Rajanikanta Mahapatra. "An In-silico attempt to catch hold of the novel microRNAs in the Bio Energy Plant (Jatropha curcus): A Big Search." Biomirror 3, no. 07 (2012): 21–24. https://doi.org/10.5281/zenodo.30713.

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The oil-rich and weedy plant Jatropha has been hailed as the most promising source of biofuel on the planet, as a non-food, droughtresistant and oil-rich crop, Jatropha curcas fulfils many of the requirements for biofuel industries. A better understanding of the biochemical pathway leading to the synthesis of Jatropha oil and its regulation both by exogenous and endogenous factors is essential for facilitating increased yield. Increasing evidence has shown that miRNAs play multiple roles in various biological processes. The research finds previously known miRNAs from various plant species expressed sequence tags (EST) database to search for potential miRNAs and their targets in Jatropha curcus. Here we present an EST (Expressed Sequence Tags) based homology search approach for the detection of miRNAs and their targets in Jatropha curcas using previously known miRNA sequences from Oryza sativa, Arabidopsis thaliana, Populus trichocarpa, Ricinus communis, Citrus sinensis, Vitis vinifera. We find presence of no miRNAs. What can be the cause?
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Livingstone, J. M., P. Seguin, and M. V. Strömvik. "An in silico study of the genes for the isoflavonoid pathway enzymes in soybean reveals novel expressed homologues." Canadian Journal of Plant Science 90, no. 4 (2010): 453–69. http://dx.doi.org/10.4141/cjps08214.

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Soybean [Glycine max (L.) Merr.] is an important source of isoflavones used by the nutraceutical industry. The soybean genome (2n = 40, 975 Mb) has recently been sequenced, and over a million (redundant) gene tags (expressed sequence tags, ESTs) are available in public databases. Using bioinformatics, we investigated five key enzymes of the isoflavonoid pathway (i.e., chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavanone dehydratase, isoflavanone-7-O-glycosyltransferase, and isoflavone-7-O-glucoside-6′′-O-malonyltransferase) to gain a better understanding of which gene homologues are expressed. Contiguous sequences (contigs) were assembled from EST data to represent the specific genes and were subsequently used to predict and verify known and novel gene homologues in the recently released chromosome-based assembly of the soybean genome. Novel transcripts for 2-hydroxyisoflavanone dehydratase and isoflavone-7-O-glucosyltransferase were discovered in these data and in silico expression profiles are presented for all the genes identified in the isoflavonoid pathway. Key words: Soybean, expressed sequence tag, isoflavonoid, gene expression, homologues
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Burki, Fabien, Sergey I. Nikolaev, Ignacio Bolivar, Jackie Guiard, and Jan Pawlowski. "Analysis of expressed sequence tags from a naked foraminiferan Reticulomyxa filosa." Genome 49, no. 8 (2006): 882–87. http://dx.doi.org/10.1139/g06-048.

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Foraminifers are a major component of modern marine ecosystems and one of the most important oceanic producers of calcium carbonate. They are a key phylogenetic group among amoeboid protists, but our knowledge of their genome is still mostly limited to a few conserved genes. Here, we report the first study of expressed genes by means of expressed sequence tag (EST) from the freshwater naked foraminiferan Reticulomyxa filosa. Cluster analysis of 1630 valid ESTs enabled the identification of 178 groups of related sequences and 871 singlets. Approximately 50% of the putative unique 1059 ESTs could be annotated using Blast searches against the protein database SwissProt + TrEMBL. The EST database described here is the first step towards gene discovery in Foraminifera and should provide the basis for new insights into the genomic and transcriptomic characteristics of these interesting but poorly understood protists.Key words: Rhizaria, Foraminifera, cDNA library, annotation.
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Dissertations / Theses on the topic "Expressed sequence tags (EST)"

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Zhang, Yuan. "MST Based Ab Initio Assembler of Expressed Sequence Tags." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1273245641.

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Alexsson, Andrei. "Unsupervised hidden Markov model for automatic analysis of expressed sequence tags." Thesis, Linköpings universitet, Bioinformatik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-69575.

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This thesis provides an in-depth analyze of expressed sequence tags (EST) that represent pieces of eukaryotic mRNA by using unsupervised hidden Markov model (HMM). ESTs are short nucleotide sequences that are used primarily for rapid identificationof new genes with potential coding regions (CDS). ESTs are made by sequencing on double-stranded cDNA and the synthesizedESTs are stored in digital form, usually in FASTA format. Since sequencing is often randomized and that parts of mRNA contain non-coding regions, some ESTs will not represent CDS.It is desired to remove these unwanted ESTs if the purpose is to identifygenes associated with CDS. Application of stochastic HMM allow identification of region contents in a EST. Softwares like ESTScanuse HMM in which a training of the HMM is done by supervised learning with annotated data. However, because there are not always annotated data at hand this thesis focus on the ability to train an HMM with unsupervised learning on data containing ESTs, both with and without CDS. But the data used for training is not annotated, i.e. the regions that an EST consists of are unknown. In this thesis a new HMM is introduced where the parameters of the HMM are in focus so that they are reasonablyconsistent with biologically important regionsof an mRNA such as the Kozak sequence, poly(A)-signals and poly(A)-tails to guide the training and decoding correctly with ESTs to proper statesin the HMM. Transition probabilities in the HMMhas been adapted so that it represents the mean length and distribution of the different regions in mRNA. Testing of the HMM's specificity and sensitivityhave been performed via BLAST by blasting each EST and compare the BLAST results with the HMM prediction results.A regression analysis test shows that the length of ESTs used when training the HMM is significantly important, the longer the better. The final resultsshows that it is possible to train an HMM with unsupervised machine learning but to be comparable to supervised machine learning as ESTScan, further expansion of the HMM is necessary such as frame-shift correction of ESTs byimproving the HMM's ability to choose correctly positioned start codons or nucleotides. Usually the false positive results are because of incorrectly positioned start codons leadingto too short CDS lengths. Since no frame-shift correction is implemented, short predicted CDS lengths are not acceptable and is hence not counted as coding regionsduring prediction. However, when there is a lack of supervised models then unsupervised HMM is a potential replacement with stable performance and able to be adapted forany eukaryotic organism.
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Godwin, Michael Jason. "Molecular Mapping of Disease-Related Expressed Sequence Tags and Resistance Gene Analogues in Soybean." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/35550.

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Soybean has become one of the most important crops to the United States, resulting in a need to improve its disease resistance. The objectives of this study were to 1) design primers and develop PCR-based markers from disease-related expressed sequence tags (ESTs) and resistance gene analogues (RGAs), 2) assess the utility of such markers by diversity analysis of 12 soybean parental lines, and 3) search for possible association of the markers with known disease resistance genes by constructing a linkage map. The diversity analysis will allow this study to determine how well each marker can distinguish genotypes in soybean. Identifying the location of our markers in the soybean genome with the linkage map, will allow those related to disease resistance to be selected. A total of 202 simple sequence length polymorphism (SSLP) markers were constructed using a set of 1218 disease-related ESTs. Furthermore, 22 markers were constructed using previously identified RGA sequences. Both sets of markers were able to detect polymorphism in the diversity analysis. Also, 48 of the SSLPs, five of the RGAs, and 150 molecular markers were used to construct a soybean linkage map using 114 recombinant inbred lines (RILs). Several markers mapped to chromosomal regions known to contain disease resistance genes. This study has created a framework map, which will be useful for identifying the location of resistance genes, marker-assisted selection for resistance, discovering novel resistance genes, and understanding genome organization of resistance pathways in soybean. An effective approach to develop "candidate gene" markers has been demonstrated.<br>Master of Science
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Alcalde, Felipe Santiago Chambergo. "Elucidação do destino metabólico de glicose no fungo filamentoso Trichoderma reesei por análise EST (Expressed Sequence Tags) e "microarrays" de cDNA." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-11022003-130558/.

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Apesar do intenso interesse na regulação metabólica e evolução das vias produtoras de ATP, o porquê de a maioria dos microorganismos multicelulares metabolizarem glicose através de respiração, ao invés da fermentação, ainda permanece sem resposta. Um desses microorganismos é o fungo celulolítico Trichoderma reesei (Hypocrea jecorina. Usando análise EST e microarrays de cDNA, foi estabelecido, em T. reesei, que a expressão dos genes que codificam as enzimas do ciclo de TCA é programada de tal modo a favorecer a oxidação de piruvato pelo ciclo de TCA, ao invés de sua redução a etanol, através da fermentação. Além disso, os resultados indicam que acetaldeído pode ser convertido a acetato, e não a etanol, prevenindo a regeneração de NAD+, um produto chave requerido para o metabolismo anaeróbico. Os estudos também mostram que a maquinaria de controle regulatório por glicose, foi, provavelmente, objeto de pressão evolutiva, a qual dirigiu o fluxo metabólico à respiração, e não à fermentação.<br>Despite the intense interest in the metabolic regulation and evolution of the ATP-producing pathways, the long-standing question of why most multicellular microorganisms metabolize glucose by respiration rather than fermentation remains unanswered. One such microorganism is the cellulolytic fungus Trichoderma reesei (Hypocrea jecorina). Using EST analysis and cDNA microarrays, we find that in T. reesei expression of the genes encoding the enzymes of the TCA is programmed in a way that favors the oxidation of pyruvate via the TCA cycle rather than its reduction to ethanol by fermentation. Moreover, the results indicate that acetaldehyde may be channeled into acetate rather than ethanol, thus preventing the regeneration of NAD+, a pivotal product required for anaerobic metabolism. The studies also point out that the regulatory machinery controlled by glucose was most probably the target of evolutionary pressure that directed the flow of metabolites into respiratory metabolism rather than fermentation.
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Koo, Hyun Jo, Eric McDowell, Xiaoqiang Ma, et al. "Ginger and turmeric expressed sequence tags identify signature genes for rhizome identity and development and the biosynthesis of curcuminoids, gingerols and terpenoids." BioMed Central, 2013. http://hdl.handle.net/10150/610084.

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BACKGROUND:Ginger (Zingiber officinale) and turmeric (Curcuma longa) accumulate important pharmacologically active metabolites at high levels in their rhizomes. Despite their importance, relatively little is known regarding gene expression in the rhizomes of ginger and turmeric.RESULTS:In order to identify rhizome-enriched genes and genes encoding specialized metabolism enzymes and pathway regulators, we evaluated an assembled collection of expressed sequence tags (ESTs) from eight different ginger and turmeric tissues. Comparisons to publicly available sorghum rhizome ESTs revealed a total of 777 gene transcripts expressed in ginger/turmeric and sorghum rhizomes but apparently absent from other tissues. The list of rhizome-specific transcripts was enriched for genes associated with regulation of tissue growth, development, and transcription. In particular, transcripts for ethylene response factors and AUX/IAA proteins appeared to accumulate in patterns mirroring results from previous studies regarding rhizome growth responses to exogenous applications of auxin and ethylene. Thus, these genes may play important roles in defining rhizome growth and development. Additional associations were made for ginger and turmeric rhizome-enriched MADS box transcription factors, their putative rhizome-enriched homologs in sorghum, and rhizomatous QTLs in rice. Additionally, analysis of both primary and specialized metabolism genes indicates that ginger and turmeric rhizomes are primarily devoted to the utilization of leaf supplied sucrose for the production and/or storage of specialized metabolites associated with the phenylpropanoid pathway and putative type III polyketide synthase gene products. This finding reinforces earlier hypotheses predicting roles of this enzyme class in the production of curcuminoids and gingerols.CONCLUSION:A significant set of genes were found to be exclusively or preferentially expressed in the rhizome of ginger and turmeric. Specific transcription factors and other regulatory genes were found that were common to the two species and that are excellent candidates for involvement in rhizome growth, differentiation and development. Large classes of enzymes involved in specialized metabolism were also found to have apparent tissue-specific expression, suggesting that gene expression itself may play an important role in regulating metabolite production in these plants.
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Pollock, Stephanie. "A study of genetic diversity and genome organization of Brassica napus using EST (expressed sequence tags) of Arabidopsis and SSR (simple sequence repeat) markers of B. napus /." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33023.

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Arabidopsis expressed sequence tags (ESTs) and microsatellites of Brassica napus have been developed and used as PCR-based markers for both mapping and genetic diversity studies in B. napus . Out of 300 random Arabidopsis ESTs screened, 43 markers were mapped onto a genetic map of B. napus and then used in a diversity study involving 48 B. napus cultivars. A second set of EST markers were developed from chromosome 1 of Arabidopsis and used in genetic mapping studies of B. napus. From 192 primer pairs developed, 50 markers were added onto the B. napus reference map. Microsatellite markers were developed using a "GA" enriched genomic library from B. napus. From 152 designed primer pairs, 23 markers were added onto the B. napus reference map. Microsatellite markers were also used in genetic diversity studies of B. napus, where, from the 152 primer pairs, 40 revealed polymorphism between the 48 B. napus cultivars.
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Esteves, Michelle Pereira. "Systematic analysis of expressed sequence tag (EST) databases to identify novel breast markers in breast cancer." Thesis, Royal Holloway, University of London, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.537516.

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Silva, Marcicleide Lima da. "Estudo de genes expressos em frutos de camu-camu: seqüenciamento de ests." Universidade Federal do Amazonas, 2006. http://tede.ufam.edu.br/handle/tede/4385.

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Submitted by Alisson Mota (alisson.davidbeckam@gmail.com) on 2015-07-13T19:18:49Z No. of bitstreams: 1 Tese - Marcicleide Lima do Espirito Santo.pdf: 7339955 bytes, checksum: 22519cc9c5e5f14e2991f1e20ddb938f (MD5)<br>Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-15T18:14:34Z (GMT) No. of bitstreams: 1 Tese - Marcicleide Lima do Espirito Santo.pdf: 7339955 bytes, checksum: 22519cc9c5e5f14e2991f1e20ddb938f (MD5)<br>Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-07-15T18:19:56Z (GMT) No. of bitstreams: 1 Tese - Marcicleide Lima do Espirito Santo.pdf: 7339955 bytes, checksum: 22519cc9c5e5f14e2991f1e20ddb938f (MD5)<br>Made available in DSpace on 2015-07-15T18:19:56Z (GMT). No. of bitstreams: 1 Tese - Marcicleide Lima do Espirito Santo.pdf: 7339955 bytes, checksum: 22519cc9c5e5f14e2991f1e20ddb938f (MD5) Previous issue date: 2006-06-22<br>CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>The camu-camu (Myrciaria dubia (H.B.K.) McVaugh) is a native sort of the Amazonian region, whose fruit presents elevated content of ascorbic acid (vitamin C). The study of the functional genome in camu-camu fruits has like base the expressed sequence tags sequencing - ESTs. Faced with the displayed the present thesis is going to analyze and identify express genes in camu-camu fruits by means of ESTs sequencing. The total RNA was extracted from the shell-pulp. The extremity 5’sequencing' of cDNA insert was carried out so much in the Technology of the DNA Laboratory (UFAM) and in the Sequencing Platform (EMBRAPA/CENARGEN). The ESTs sequences obtained were submitted to the System Genome, program of genomic annotation that integrates analysis management programs and viewing of nucleotides sequences. It developed an efficient procedure for total RNA extraction of camu-camu fruits that enabled the obtaining of mRNAs of quality, utilized in the making of cDNAs of sizes varied (500pb to 4Kb). From the sequencing were obtained 3196 ESTs valid, being formed 1546 singletons and 358 contigs, resulting of 2586 ESTs sequences in total with similarity the sequences found in the gene bank. The analysis library clusterization revealed an index of 81% novelty and 32,54% redundancy. Around 90% of the contigs presented decrease redundancy (2-4 reads by contigs). The facts of the categorization of the proteins identified detached the posttranslational modification, protein turnover, chaperones (13,2%) category. From the hoist of the species with bigger number of ESTs with similarity the camu-camu sequences detached itself Arabidopsis thaliana with 49%. Around 10 uniques presented very high similarity (and-value 0.0) to known genes. The ESTs more abundantly express in camu-camu fruits encode to gluthatione s-transferase. They were observed around 3% sequences (97 ESTs) with decrease similarity (e-value > e-10) and 15% did not they present similarity with no contained sequence in the gene bank. They were identified 138 ESTs sequences (4,3%) that they encode molecular chaperones with prevalence of the sHSP family that represents 33% of express chaperones. ESTs related to the ascorbic acid metabolism also were identified, being nine related the synthesis and six come back for ascorbic acid conversion and recycling. ESTs related to the ripening and mechanisms of defense of the fruit also were noticeable.<br>O camu-camu (Myrciaria dúbia (H.B.K.) McVaugh) é uma espécie nativa da região Amazônica, cujo fruto apresenta elevado teor de ácido ascórbico (vitamina C). O estudo do genoma funcional em frutos de camu-camu tem como base o seqüenciamento de fragmentos de seqüências expressas - ESTs (Expressed Sequence Tags). Diante do exposto a presente tese pretende analisar e identificar genes expressos em frutos de camu-camu por meio de seqüenciamento de ESTs. O RNA total foi extraído a partir da casca-polpa. O seqüenciamento da extremidade 5’ de insertos de cDNA foi realizado tanto no Laboratório de Tecnologia do DNA da UFAM e como na Plataforma de Seqüenciamento da EMBRAPA/CENARGEN. As seqüências ESTs obtidas foram submetidas ao Sistema Genoma, programa de anotação genômica que integra programas de gerenciamento de análise e visualização de seqüências nucleotídicas. Os resultados obtidos foram o desenvolvimento de um procedimento eficiente para extração de RNA total de frutos de camu-camu que possibilitou a obtenção de mRNAs de qualidade, utilizados na confecção de cDNAs de tamanhos variados (500pb a 4Kb). A partir do seqüenciamento foram obtidas 3196 ESTs válidas, sendo formados 1546 singletons e 358 contigs, resultando num total de 2586 seqüências ESTs com similaridade a seqüências encontradas no banco de genes. A análise da clusterização da biblioteca revelou um índice de 81% de novidade e 33% de redundância. Cerca de 90% dos contigs apresentaram baixa redundância (2-4 reads por contigs). Os dados da categorização das proteínas identificadas destacaram a categoria modificação pós-traducional, proteína turnover, chaperonas (13,2%). A partir do levantamento das espécies com maior número de ESTs com similaridade a seqüências de camu-camu destacou-se Arabidopsis thaliana com 49%. Cerca de 10 uniques apresentaram altíssima similaridade (e-value 0.0) a genes conhecidos. Os ESTs mais abundantemente expresso em frutos de camu-camu codificam a glutationa s-transferase. Foram observados cerca de 3% de seqüências (97 ESTs) com baixa similaridade (e-value > e-1010) e 15% não apresentaram similaridade com nenhuma seqüência contida no banco de genes. Foram identificadas 138 seqüências ESTs (4,3%) que codificam chaperonas moleculares com destaque à família sHSP que representa 33% das chaperonas expressas. ESTs relacionados ao metabolismo do ácido ascórbico também foram identificados, sendo nove relacionados a síntese e seis voltados para conversão e reciclagem do ácido ascórbico. ESTs relacionados ao amadurecimento e mecanismos de defesa do fruto também foram destacados.
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Waikel, Patricia A. "Analysis of a Partial Expressed Sequence Tag (EST) Library and Differential Expression of Genes in Biochemical Morphotypes of the Marine Sponge Discodermia dissoluta." NSUWorks, 2010. http://nsuworks.nova.edu/occ_stuetd/197.

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A variety of secondary metabolites with promising antimicrobial and anti-tumor properties have been identified in marine organisms. Sponges, in particular, have been the source of several of these, including discodermolide from Discodermia dissoluta. While metagenomic studies have been undertaken to identify genes involved in discodermolide production, presently, a transcriptomic approach has not been taken to characterize the metagenome of D. dissoluta. Samples of D. dissoluta were collected from a site in the Bahamas and screened for secondary metabolite production. Some specimens of D. dissoluta were positive for discodermolide while others were not. In order to determine which genes are differentially expressed between the two specimens, suppression subtractive hybridization (SSH) was performed utilizing a chemistry negative and chemistry positive morphotype as the “driver” and “tester” populations respectively. Here we demonstrate the efficacy of SSH through the identification of transcripts related to symbiosis and secondary metabolite production by metatranscriptomic and bioinformatics analyses of the resulting subtracted library as well as a 16S rRNA library. Additionally, we have confirmed differential gene expression of selected sequences utilizing quantitative polymerase chain reaction (qPCR) with SYBR Green chemistry to screen and characterize genes, some of which appear to be related to novel metabolism and unknown functions related to symbiosis within the complex sponge-microbial community.
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Alves, Helena Javiel. "Identificação e caracterização de seqüências expressas (EST) na musculatura peitoral de frangos de corte." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-11012005-161916/.

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A produção de aves no Brasil vem crescendo na ordem de 10% a cada ano, o que se explica pela atualização constante da tecnologia do setor (http://www.abef.com.br). Sendo a carne de frango a fonte de proteína animal mais barata e acessível ao consumidor, há necessidade de se produzir cada vez mais animais com maior acúmulo de massa muscular. Para isso, o entendimento dos mecanismos celulares e moleculares envolvidos na formação da musculatura esquelética é de extrema relevância. Os fatores miogênicos, genes responsáveis pela determinação e diferenciação de células musculares, foram clonados e progressos significativos foram desenvolvidos quanto ao controle da expressão dos mesmos. A utilização da técnica de seqüenciamento de DNA possibilita a identificação e caracterização de novos genes envolvidos na complexa rede de fatores que regulam a formação da musculatura esquelética em aves. Neste estudo, foram construídas duas bibliotecas de cDNA (fase embrionária e pós-eclosão) de músculo peitoral de uma linhagem de corte (TT) e uma biblioteca da fase embrionária de uma linhagem de postura (CC). A análise das seqüências EST (Expressed Sequence Tags) foi utilizada para identificar possíveis novos genes envolvidos no processo de formação da musculatura esquelética. As seqüências EST identificadas possibilitaram a construção de um banco com 6247 ESTs da musculatura peitoral das linhagens de corte e postura nas duas fases de desenvolvimento. Com o intuito de estabelecer uma relação entre o perfil de expressão dos fatores miogênicos: MyoD, MRF4 e miogenina; e dos genes Pax-3 e miostatina e a formação e maturação das fibras musculares, foi utilizada a técnica de PCR em tempo real. Em geral, a expressão dos fatores miogênicos foi maior na linhagem de corte em relação à de postura nas idades estudadas. Este estudo deverá contribuir para as áreas celular e molecular de desenvolvimento, além de fornecer recursos úteis aos programas de melhoramento genético de aves que visam obter animais com maior acúmulo de massa muscular.<br>Brazilian’s chicken production is increasing annually around 10%, which can be explained by the current technology applied to this sector (http://www.abef.com.br). Being chicken’s meat the cheapest animal protein source for consumers, there is a need to produce even more animals with increased muscular mass. For this purpose, understanding the molecular and cellular mechanisms involved with the skeletal muscle development is of great relevance. The myogenic factors, genes responsible for the determination and differentiation of muscle cells, were cloned and significant progress was made on the control of their expression. The use of DNA sequencing technique allows the identification and characterization of new genes involved in the complex chain of factors signalling systems that regulates the expression of avian skeletal muscles. In this study, two cDNA libraries (embryonic and post-hatching phases) were constructed from the breast muscle of a chicken broiler line (TT) and one library, from the embryonic phase, from a chicken layer line (CC). The EST (Expressed Sequencing Tags) analysis was used to identify probable new genes involved in the skeletal muscle development. The identified ESTs were used to generate a database containing 6247 breast muscle ESTs from two chicken lines in two development phases. Real time PCR was employed with the aim of establishing a relationship among the expression profile of myogenic factors (MyoD, MFR4, and myogenin), Pax-3 and myostatin genes with the formation and maturation of muscle fibers. In general, the expression of myogenic factors was greater in the broiler than in the layer chicken line in the phases under study. These results should contribute to other cellular and molecular development studies besides providing useful resources for chicken breeding programs whose objective is the deposition of skeletal muscle mass.
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Books on the topic "Expressed sequence tags (EST)"

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Parkinson, John, ed. Expressed Sequence Tags (ESTs). Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-136-3.

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Rune, Matthiesen, ed. Mass spectrometry data analysis in proteomics. Humana Press, 2007.

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Matthiesen, Rune. Mass spectrometry data analysis in proteomics. Humana Press, 2013.

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Expressed Sequence Tags (ESTs). Humana Press, 2008.

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Parkinson, John. Expressed Sequence Tags: Generation and Analysis. Humana Press, 2010.

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Expressed Sequence Tags: Generation and Analysis. Humana Press, 2009.

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Matthiesen, Rune. Mass Spectrometry Data Analysis in Proteomics. Springer New York, 2019.

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Matthiesen, Rune. Mass Spectrometry Data Analysis in Proteomics. Humana Press, 2010.

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Matthiesen, Rune. Mass Spectrometry Data Analysis in Proteomics. Springer New York, 2020.

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Matthiesen, Rune. Mass Spectrometry Data Analysis in Proteomics. Humana Press, 2016.

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Book chapters on the topic "Expressed sequence tags (EST)"

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Al-Faifi, Sulieman A., Hussein M. Migdadi, Salem S. Algamdi, et al. "Analysis of Expressed Sequence Tags (EST) in Date Palm." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7159-6_23.

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Pedretti, Kevin, Todd Scheetz, Terry Braun, Chad Roberts, Natalie Robinson, and Thomas Casavant. "A Parallel Expressed Sequence Tag (EST) Clustering Program." In Lecture Notes in Computer Science. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/3-540-44743-1_51.

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Clifton, Sandra W., and Makedonka Mitreva. "Strategies for Undertaking Expressed Sequence Tag (EST) Projects." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-136-3_2.

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Schmitt, Armin O. "Mining Expressed Sequence Tag (EST) Libraries for Cancer-Associated Genes." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-545-9_6.

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Joshi, Aruna G., and Ashutosh R. Pathak. "EST (Expressed Sequence Tag): A Technique for Identification of Plant Secondary Metabolite Genes." In Plant and Human Health, Volume 2. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-03344-6_8.

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Wolfsberg, Tyra G., and David Landsman. "Expressed Sequence Tags (ESTs)." In Methods of Biochemical Analysis. John Wiley & Sons, Inc., 2002. http://dx.doi.org/10.1002/0471223921.ch12.

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Parkinson, John, and Mark Blaxter. "Expressed Sequence Tags: An Overview." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-136-3_1.

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Susko, Edward, and Andrew J. Roger. "Statistical Analysis of Expressed Sequence Tags." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-136-3_13.

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Cheung, Foo. "Global Assembly of Expressed Sequence Tags." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-839-9_15.

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Wasmuth, James, and Mark Blaxter. "Obtaining Accurate Translations from Expressed Sequence Tags." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-136-3_10.

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Conference papers on the topic "Expressed sequence tags (EST)"

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Keng-Hoong Ng, Somnuk Phon-Amnuaisuk, and Chin-Kuan Ho. "Clustering of Expressed Sequence Tag (EST) with Markov models and self-organizing maps: An exploratory study." In 2008 International Symposium on Information Technology. IEEE, 2008. http://dx.doi.org/10.1109/itsim.2008.4631585.

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Reena, N., A. Chandrasekar, A. Riju, P. L. Nima, S. J. Eapen, and M. Anandaraj. "Gene identification inPhytophthora capsicithrough expressed sequence tags." In the International Symposium. ACM Press, 2010. http://dx.doi.org/10.1145/1722024.1722043.

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Adams, Mark D., Anthony R. Kerlavage, Mark Dubnick, Ruben F. Moreno, Chris Fields, and J. Craig Venter. "ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM HUMAN BRAIN CDNAS." In Proceedings of the 2nd International Conference. WORLD SCIENTIFIC, 1993. http://dx.doi.org/10.1142/9789814503655_0010.

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Min, Xiangjia J., Gregory Butler, Reginald Storms, and Adrian Tsang. "Comparative Assessment of DNA Assemblers for Assembling Expressed Sequence Tags." In 2009 Ohio Collaborative Conference on Bioinformatics (OCCBIO). IEEE, 2009. http://dx.doi.org/10.1109/occbio.2009.19.

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Sim-Hui Tee. "Identification of the overexpressed genes in hepatocellular carcinoma using Expressed Sequence Tags." In 2012 International Conference on Biomedical Engineering (ICoBE). IEEE, 2012. http://dx.doi.org/10.1109/icobe.2012.6179078.

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Lee, Guo-Hsing, Nai-Yu Chuang, Wen-Dar Lin, Chung-Der Hsiao, Hahn-Ming Lee, and Jan-Ming Ho. "E2D: A Novel Tool for Annotating Protein Domains in Expressed Sequence Tags." In 2006 IEEE Symposium on Computational Intelligence and Bioinformatics and Computational Biology. IEEE, 2006. http://dx.doi.org/10.1109/cibcb.2006.330967.

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Jain, M., H. Holz, J. Shrager, O. Vallon, C. Hauser, and A. Grossman. "A Hybrid, Recursive Algorithm for Clustering Expressed Sequence Tags in Chlamydomonas reinhardtii." In 18th International Conference on Pattern Recognition (ICPR'06). IEEE, 2006. http://dx.doi.org/10.1109/icpr.2006.87.

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Gong, Ping, Fuliang Xie, Baohong Zhang, and Edward J. Perkins. "In silico identification of microRNAs from expressed sequence tags of three earthworm species." In the First ACM International Conference. ACM Press, 2010. http://dx.doi.org/10.1145/1854776.1854847.

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Wati, Ridha, Mohammad Basyuni, Shigeyuki Baba, and Hirosuke Oku. "Bioinformatics analysis of the expressed sequence tags from Rhizophora stylosa Griff. genomic library." In INVENTING PROSPEROUS FUTURE THROUGH BIOLOGICAL RESEARCH AND TROPICAL BIODIVERSITY MANAGEMENT: Proceedings of the 5th International Conference on Biological Science. Author(s), 2018. http://dx.doi.org/10.1063/1.5050147.

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Ng, Keng-Hoong, Somnuk Phon-Amnuaisuk, and Chin-Kuan Ho. "Clustering of expressed sequence tags with distance measure based on Burrows-Wheeler transform." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639798.

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Reports on the topic "Expressed sequence tags (EST)"

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อิ่มจงใจรัก, จันทร์ประภา. การแยกและการศึกษาลักษณะสมบัติของเปปไทด์ต้านจุลชีพจากเซลล์เม็ดเลือดของปูทะเล Scylla paramamosain : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.52.

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เปปไทด์ต้านจุลชีพเป็นเปปไทด์สายสั้นที่มีความสำคัญต่อระบบภูมิคุ้มกันของร่างกายของสิ่งมีชีวิตหลายชนิดโดยมีคุณสมบัติที่สามารถต่อต้านการติดเชื้อที่เกิดจากจุลชีพได้ ในงานวิจัยนี้ได้ทำการค้นหาลำดับนิวคลีโอไทด์ที่สมบูรณ์(full length cDNA) ของยีนเปปไทด์ต้านจุลชีพครัสติน(CrusSp)ศึกษาลักษณะสมบัติของยีนรวมทั้งแยกและวิเคราะห์การจัดเรียงตัวของยีน(genomicorganization)จากการค้นหาลำดับนิวคลีโอไทด์ที่สมบูรณ์ของยีนเปปไทด์ต้านจุลชีพครัสตินจากเซลล์เม็ดเลือดแดงของปูทะเล(Scylla paramamosain) โดยเทคนิค expressed sequence tag(EST) และ rapid amplification cDNA end (RACE) พบว่าประกอบด้วย ORF ขนาด 336 bp ที่สามารถถอดรหัสให้กรดอะมิโน 11 ตัว โดยมี signal peptide 21 ตัว เมื่อนำมาคำนวณมวลโมเลกุลของโปรตีนพบว่ามีขนาดประมาณ 10.27 kDa และมีค่า pI 8.54 จากการวิเคราะห์โดเมนของโปรตีน CrusSp พบว่าที่บริเวณปลาย C ประกอบด้วย whey acidic protein (WAP) domain จากการเปรียบเทียบลำดับกรดอะมิโนและการวิเคราะห์ความสัมพันธ์เชิงวิวัฒนาการพบว่ายีน CrusSp ที่แยกได้มีความคล้ายกับกลุ่มของยีนครัสตินโดยมีความสัมพันธ์ใกล้ชิดกับเปปไทด์ต้านจุลชีพครัสตินที่พบในปูCarcinus maenas จากการแยกและวิเคราะห์การจัดเรียงตัวของยีนพบว่ายีน CrusSp มีขนาด 999 bp ซึ่งประกอบด้วย 4 exon และ 3 intron โดยในบริเวณระหว่างรอยต่อระหว่าง exon และ intron เป็นไปตามGT/AG rule เมื่อตรวจสอบการแสดงออกของยีน CrusSp ในเนื้อเยื่อต่างๆของปูทะเลพบว่ายีน CrusSp มีการแสดงออกอย่างมากในเซลล์เม็ดเลือด เหงือก ลำไส้ และกล้ามเนื้อ แต่ไม่พบการแสดงออกในตับและก้านตา เมื่อนำยีน CrusSp มาศึกษาการแสดงออกใน Escherichia coli พบว่าสามารถเหนี่ยวนำให้มีการแสดงออกของโปรตีนรีคอมบิแนนท์ได้ เมื่อนำมาแยกบริสุทธิ์และตรวจสอบคุณสมบัติการต้านเชื้อจุลชีพพบว่าโปรตีน CrusSpมีฤทธิ์ในการต้านเชื้อแบคทีเรียแกรมบวกได้
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2

นุชประยูร, อิศรางค์, มณฑน์มาศ สุนทราวัฒน์ та ธัญณิชา อ่อนดี. การศึกษาโปรตีน ที่สำคัญในพิษงูแมวเซาเพื่อนำมาดัดแปลงใช้ประโยชน์ทางการแพทย์ : รายงานการวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.23.

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ปัญหางูพิษกัดเป็นปัญหาทางสาธารณะสุขที่สำคัญของประเทศไทย หนึ่งในงูพิษที่สำคัญในไทยคืองูแมวเซา (Daboia russellii siamensis) งูชนิดนี้พบมากในแถบภาคกลางและภาคตะวันออกของไทย ผู้ที่ถูกงูชนิดนี้กัด มักมีอาการทางระบบเลือด และภาวะไตวายเฉียบพลัน ซึ่งเป็นสาเหตุสำคัญที่ทำให้ผู้ที่ถูกงูแมวเซากัดเสียชีวิต ในปัจจุบันความรู้เกี่ยวกับกลไกการเกิดพิษหลังถูกงูแมวเซากัด รวมทั้งโปรตีนสำคัญในพิษงูแมวเซาที่อาจมีประโยชน์ทางการแพทย์ ยังไม่มีการศึกษาอย่างแน่ชัด การศึกษาองค์ประกอบของพิษงูแมวเซาในเชิงลึก จะช่วยให้เข้าใจกลไกการเกิดพิษ นำไปสู่การรักษาที่มีประสิทธิภาพที่ดีขึ้น พร้อมทั้งอาจนำความรู้ที่ได้ไปใช้ประโยชน์ในทางการแพทย์ต่อไป จากผลงานที่ผ่านมา ทางกลุ่มผู้วิจัยได้สร้างห้องสมุดยีน (cDNA library) จากต่อมพิษงูแมวเซาได้สำเร็จ และได้มีการศึกษาองค์ประกอบของพิษงูเบื้องต้นด้วยเทคนิค Expressed Sequence Tags Analysis พบว่าในพิษงูมีการแสดงออกของยีนที่เกี่ยวข้องกับกระบวนการ haematostasis เป็นจำนวนมาก ซึ่งสัมพันธ์กับความเป็นพิษของงูแมวเซาที่มีผลโดยตรงต่อระบบเลือดของเหยื่อที่ถูกกัด หลังจากการศึกษาวิจัยในปีแรกได้ทำการแยกบริสุทธิ์โปรตีนพิษงูแมวเซาชนิด RVV-X และ PLA2 ก่อนโดยใช้วิธี Gel filtration column chromatography และ Anion exchange column chromatography และทำโคลนและผลิตโปรตีนที่พบใน cDNA library ด้วย recombinant technology จำนวน 5 ยีน ได้สำเร็จ คือ phospholipase A2 (PLA2), factor X activating enzyme (RVV-X), factor V activating enzyme (RVV-V), serine β-fibrinogenase และ rJerdostatin homolog (Daboistatin short disintegrin) โดยที่โปรตีนทุกตัวสามารถทำปฏิกิริยากับ anti his tag ได้ โดยในการศึกษานี้เป็นการทดสอบคุณสมบัติทางชีวเคมีของโปรตีน RVV-X ทั้งในหลอดทดลองและในสัตว์ทดลอง รวมถึงการทดสอบการทำงานของ recombinant protein ที่ผลิตได้ ซึ่งสามารถนำความรู้ที่ได้ไปใช้ประโยชน์ในทางการแพทย์ต่อไป
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นุชประยูร, อิศรางค์. การศึกษาโปรตีนที่สำคัญในพิษงูแมวเซาเพื่อนำมาดัดแปลงใช้ประโยชน์ทางการแพทย์ : รายงานการวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์มหาวิทยาลัย, 2008. https://doi.org/10.58837/chula.res.2008.26.

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Abstract:
ปัญหางูพิษกัดเป็นปัญหาทางสาธารณสุขที่สำคัญของประเทศไทย หนึ่งในงูพิษที่สำคัญในไทยคือ งูแมวเซา (Daboia russellii siamensis) งูชนิดนี้พบมากในแถบภาคกลางและภาคตะวันออกของไทย ผู้ที่ถูกงูชนิดนี้กัด มักมีอาการทางระบบเลือด และภาวะไตวายเฉียบพลัน ซึ่งเป็นสาเหตุสำคัญที่ทำให้ผู้ที่ถูกงูแมวเซากัดเสียชีวิต ในปัจจุบันความรู้เกี่ยวกับกลไกการเกิดพิษหลังถูกงูแมวเซากัด รวมทั้งโปรตีนสำคัญในพิษงูแมวเซาที่อาจมีประโยชน์ทางการแพทย์ ยังไม่มีการศึกษาอย่างแน่ชัด การศึกษาองค์ประกอบของพิษงูแมวเซาในเชิงลึก จะช่วยให้เข้าใจกลไกการเกิดพิษ นำไปสู่การรักษาที่มีประสิทธิภาพที่ดีขึ้น พร้อมทั้งอาจนำความรู้ที่ได้ไปใช้ประโยชน์ในทางการแพทย์ต่อไป จากผลงานที่ผ่านมา ทางกลุ่มผู้วิจัยได้สร้างห้องสมุดยีน (cDNA library) จากต่อมพิษงูแมวเซาได้สำเร็จ และได้มีการศึกษาองค์ประกอบของพิษงูเบื้องต้นด้วยเทคนิค Expressed Sequence Tags Analysis พบว่าในพิษงูมีการแสดงออกของยีนที่เกี่ยวข้องกับกระบวนการ haematostasis เป็นจำนวนมาก ซึ่งสัมพันธ์กับความเป็นพิษของงูแมวเซาที่มีผลโดยตรงต่อระบบเลือดของเหยื่อที่ถูกกัด โปรตีนที่พบมากคือ phospholipase A2, faetor X activating enzyme (RVV-X), factor V activating enzyme (RVV-V) และโปรตีนอื่นๆ อีกหลายชนิด จากการทบทวนวรรณกรรม โปรตีนพิษงูแมวเซาที่น่าสนใจคือ RVV-X และ PLA2 ดังนั้นในการศึกษานี้จึงได้แยกโปรตีนพิษงูชนิด RVV-X และ PLA2 จากพิษงูแมวเซาของไทยก่อน โดยใช้วิธี Gel filtration column Chromatography และ Anion exchange column chromatography จากนั้นยืนยันผลการแยกบริสุทธิ์โปรตีนพิษงูชนิด RVV-X และ PLA2 และหาขนาดของโปรตีนพิษงูทั้ง 2 ชนิด โดยใช้วิธี MALDI-TOF mass spectrometry พบว่าโปรตีนพิษงูแมวเซาทั้งชนิด RVV-X และ PLA2 มีความบริสุทธิ์สูงและมีขนาดโปรตีนประมาณ 90 และ 14 Kb ใน non-reducing condition ตามลำดับ จากนั้นได้ทดสอบคุณสมบัติทางชีวเคมีของ RVV-X และ PLA2 ที่แยกบริสุทธิ์ได้ พบว่าโปรตีนทั้งสองชนิดมี enzymatic activity สูง โครงการนี้เป็นโครงการวิจัยต่อเนื่อง 3 ปี (พ.ศ. 2550-2552) ซึ่งในขณะนี้อยู่ในระหว่างการโคลนยีนต่างๆ ที่สำคัญในพิษงูแมวเซาไทยจากห้องสมุดยีน และทำการ express ยีนเหล่านี้เพื่อสร้าง recombinant proteins ที่คาดว่าจะมีความสามารถในการทำงานได้เหมือน native protein รวมทั้งศึกษาโปรตีนชนิดต่างๆ ในพิษงูที่ได้จากทั้งวิธีแยกโปรตีนในพิษงูโดยเทคนิคทางชีวเคมี หรือจาก recombinant proteins เพื่อหาโปรตีนเป้าหมายที่สำคัญต่อการกลไกการเกิดพิษหลังถูกงูแมวเซากัด และอาจนำความรู้ที่ได้ไปใช้ประโยชน์ในทางการแพทย์ต่อไป
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