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Dissertations / Theses on the topic 'Expressed sequence tags (EST)'

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Published: 3 June 2025
Last updated: 31 July 2025

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1

Zhang, Yuan. "MST Based Ab Initio Assembler of Expressed Sequence Tags." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1273245641.

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2

Alexsson, Andrei. "Unsupervised hidden Markov model for automatic analysis of expressed sequence tags." Thesis, Linköpings universitet, Bioinformatik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-69575.

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This thesis provides an in-depth analyze of expressed sequence tags (EST) that represent pieces of eukaryotic mRNA by using unsupervised hidden Markov model (HMM). ESTs are short nucleotide sequences that are used primarily for rapid identificationof new genes with potential coding regions (CDS). ESTs are made by sequencing on double-stranded cDNA and the synthesizedESTs are stored in digital form, usually in FASTA format. Since sequencing is often randomized and that parts of mRNA contain non-coding regions, some ESTs will not represent CDS.It is desired to remove these unwanted ESTs if the p
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3

Godwin, Michael Jason. "Molecular Mapping of Disease-Related Expressed Sequence Tags and Resistance Gene Analogues in Soybean." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/35550.

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Soybean has become one of the most important crops to the United States, resulting in a need to improve its disease resistance. The objectives of this study were to 1) design primers and develop PCR-based markers from disease-related expressed sequence tags (ESTs) and resistance gene analogues (RGAs), 2) assess the utility of such markers by diversity analysis of 12 soybean parental lines, and 3) search for possible association of the markers with known disease resistance genes by constructing a linkage map. The diversity analysis will allow this study to determine how well each marker can d
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4

Alcalde, Felipe Santiago Chambergo. "Elucidação do destino metabólico de glicose no fungo filamentoso Trichoderma reesei por análise EST (Expressed Sequence Tags) e "microarrays" de cDNA." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-11022003-130558/.

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Apesar do intenso interesse na regulação metabólica e evolução das vias produtoras de ATP, o porquê de a maioria dos microorganismos multicelulares metabolizarem glicose através de respiração, ao invés da fermentação, ainda permanece sem resposta. Um desses microorganismos é o fungo celulolítico Trichoderma reesei (Hypocrea jecorina. Usando análise EST e microarrays de cDNA, foi estabelecido, em T. reesei, que a expressão dos genes que codificam as enzimas do ciclo de TCA é programada de tal modo a favorecer a oxidação de piruvato pelo ciclo de TCA, ao invés de sua redução a etanol, através
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Koo, Hyun Jo, Eric McDowell, Xiaoqiang Ma, et al. "Ginger and turmeric expressed sequence tags identify signature genes for rhizome identity and development and the biosynthesis of curcuminoids, gingerols and terpenoids." BioMed Central, 2013. http://hdl.handle.net/10150/610084.

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BACKGROUND:Ginger (Zingiber officinale) and turmeric (Curcuma longa) accumulate important pharmacologically active metabolites at high levels in their rhizomes. Despite their importance, relatively little is known regarding gene expression in the rhizomes of ginger and turmeric.RESULTS:In order to identify rhizome-enriched genes and genes encoding specialized metabolism enzymes and pathway regulators, we evaluated an assembled collection of expressed sequence tags (ESTs) from eight different ginger and turmeric tissues. Comparisons to publicly available sorghum rhizome ESTs revealed a total of
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6

Pollock, Stephanie. "A study of genetic diversity and genome organization of Brassica napus using EST (expressed sequence tags) of Arabidopsis and SSR (simple sequence repeat) markers of B. napus /." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33023.

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Arabidopsis expressed sequence tags (ESTs) and microsatellites of Brassica napus have been developed and used as PCR-based markers for both mapping and genetic diversity studies in B. napus . Out of 300 random Arabidopsis ESTs screened, 43 markers were mapped onto a genetic map of B. napus and then used in a diversity study involving 48 B. napus cultivars. A second set of EST markers were developed from chromosome 1 of Arabidopsis and used in genetic mapping studies of B. napus. From 192 primer pairs developed, 50 markers were added onto the B. napus reference map. Microsatellite markers were
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7

Esteves, Michelle Pereira. "Systematic analysis of expressed sequence tag (EST) databases to identify novel breast markers in breast cancer." Thesis, Royal Holloway, University of London, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.537516.

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8

Silva, Marcicleide Lima da. "Estudo de genes expressos em frutos de camu-camu: seqüenciamento de ests." Universidade Federal do Amazonas, 2006. http://tede.ufam.edu.br/handle/tede/4385.

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9

Waikel, Patricia A. "Analysis of a Partial Expressed Sequence Tag (EST) Library and Differential Expression of Genes in Biochemical Morphotypes of the Marine Sponge Discodermia dissoluta." NSUWorks, 2010. http://nsuworks.nova.edu/occ_stuetd/197.

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A variety of secondary metabolites with promising antimicrobial and anti-tumor properties have been identified in marine organisms. Sponges, in particular, have been the source of several of these, including discodermolide from Discodermia dissoluta. While metagenomic studies have been undertaken to identify genes involved in discodermolide production, presently, a transcriptomic approach has not been taken to characterize the metagenome of D. dissoluta. Samples of D. dissoluta were collected from a site in the Bahamas and screened for secondary metabolite production. Some specimens of D. diss
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10

Alves, Helena Javiel. "Identificação e caracterização de seqüências expressas (EST) na musculatura peitoral de frangos de corte." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-11012005-161916/.

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A produção de aves no Brasil vem crescendo na ordem de 10% a cada ano, o que se explica pela atualização constante da tecnologia do setor (http://www.abef.com.br). Sendo a carne de frango a fonte de proteína animal mais barata e acessível ao consumidor, há necessidade de se produzir cada vez mais animais com maior acúmulo de massa muscular. Para isso, o entendimento dos mecanismos celulares e moleculares envolvidos na formação da musculatura esquelética é de extrema relevância. Os fatores miogênicos, genes responsáveis pela determinação e diferenciação de células musculares, foram clonados e p
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11

Prabhakar, Sheila. "Molecular characterization of digestive proteases of the yellow mealworm, Tenebrio molitor L." Diss., Manhattan, Kan. : Kansas State University, 2006. http://hdl.handle.net/2097/170.

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12

Jovino, Daniele Fernanda Revoredo. "Expressão gênica diferencial em palmitos de cana-de-açúcar submetida a diferentes períodos de estresse hídrico /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/92698.

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Resumo: Sob condições de estresse hídrico, a cana-de-açúcar pode sofrer mudanças fisiológicas e bioquímicas, tais como diminuição nas atividades fotoquímicas, redução da fixação de CO2 e acúmulo de osmólitos e osmoprotetores. O objetivo deste trabalho foi identificar, através da técnica de macroarranjo de cDNA, o perfil de expressão de genes promotores das diferentes vias metabólicas em palmitos da variedade de cana-de-açúcar SP80-3280 submetidas ao estresse hídrico nos dias 5, 9, 13 e 17 após o início da condição de supressão de água, sendo considerado o dia 1 como controle. Os resultados do
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13

Byers, Robert L. "Marker Discovery in Allotetraploid Cotton Using 454 Pyrosequencing." BYU ScholarsArchive, 2011. https://scholarsarchive.byu.edu/etd/2663.

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A narrow germplasm base and a complex allotetraploid genome have historically made the discovery of single nucleotide polymorphism (SNP) markers difficult in cotton (Gossypium hirsutum). We conducted a genome reduction experiment to identify SNPs from two accessions of G. hirsutum and two accessions of G. barbadense. Approximately 2 million sequence reads were assembled into contigs with an N50 of 508 bp and analyzed for SNPs. A total of 11,834 and 1,679 SNPs between the accessions G. hirsutum and G. barbadense, respectively, were identified with highly conservative parameters (a minimum read
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14

Jovino, Daniele Fernanda Revoredo [UNESP]. "Expressão gênica diferencial em palmitos de cana-de-açúcar submetida a diferentes períodos de estresse hídrico." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/92698.

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Made available in DSpace on 2014-06-11T19:26:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-20Bitstream added on 2014-06-13T18:54:09Z : No. of bitstreams: 1 jovino_dfr_me_jabo.pdf: 637580 bytes, checksum: dc449a2a52a9354da867effed1512963 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Sob condições de estresse hídrico, a cana-de-açúcar pode sofrer mudanças fisiológicas e bioquímicas, tais como diminuição nas atividades fotoquímicas, redução da fixação de CO2 e acúmulo de osmólitos e osmoprotetores. O objetivo deste trabalho foi identificar, atrav
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15

Habrylo, Olivier. "Une E. S. T. (Expressed Sequence Tag) conduit à la caractérisation, chez le houblon (Humulus lupulus), de trois inhibiteurs de xyloglucanase dont l’expression est influencée par des facteurs biotiques et abiotiques." Strasbourg, 2011. http://www.theses.fr/2011STRA6143.

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Les pathogènes fongiques sécrètent un arsenal enzymatique pour dégrader la paroi végétale des plantes hôtes. Certaines de ces enzymes peuvent être inhibées par des protéines de plante. Une famille d’inhibiteurs, ayant la capacité d’agir sur les xyloglucanases et nommée XEGIP (xyloglucan-specific endoglucanase inhibitor protein) est peu caractérisée et ce projet a permis la mise en évidence et l’étude de trois de ces protéines chez le houblon (Humulus lupulus). Des nombreuses preuves ont pu être accumulées sur le rôle potentiel de ces protéines dans la défense de la plante. À partir d’une EST (E
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16

Slater, Guy St Clair. "Algorithims for the analysis of expressed sequence tags." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621848.

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17

Brulliard, Marie. "Infidélité de transcription et carcinogénèse. Analyse bioinformatique et preuves de concept biologiques." Thesis, Vandoeuvre-les-Nancy, INPL, 2009. http://www.theses.fr/2009INPL037N/document.

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L’un des enjeux de la lutte contre le cancer réside dans la compréhension de l’hétérogénéité de la maladie. Le but de notre travail a été d’explorer l’hétérogénéité des cellules cancéreuses du point de vue de la séquence d’ARN messager. Les ESTs (ou Expressed Sequence Tags) d’origine humaine ont été alignées aux séquences de référence ARNm. Les alignements ont été exploités de manière à mesurer les variations de séquence des ESTs issues de tissus tumoraux ou non tumoraux à chaque position de chaque transcrit. L’analyse statistique mise en place a consisté à identifier les positions pour lesque
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18

Norrish, Alan Raymond. "Functional genomics : from expressed sequence tags to DNA vaccines for Leishmania." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624326.

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19

Wan, Kiew-Lian. "Expressed sequence tags : an approach to gene discovery in Toxoplasma gondii." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627602.

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20

Paraoan, Luminita. "Analysis of cDNA libraries and expressed sequence tags of retinal pigment epithelial cells." Thesis, University of Liverpool, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367080.

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21

Kawaura, Kanako. "Transcriptome analysis of common wheat using a large number of expressed sequence tags." Kyoto University, 2006. http://hdl.handle.net/2433/136637.

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22

Unneberg, Per. "Computational approaches for in-depth analysis of cDNA sequence tags." Doctoral thesis, KTH, Bioteknologi, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-23.

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Major recent improvements in biotechnology have led to an accelerated production of DNA sequences. The completion of the human genome sequence, along with the genomes of more than two hundred other species, has marked the arrival of the genome era. The ultimate goal is to understand the structure and function of genomes and their genes. This thesis has focused on the computational analysis of complementary DNA (cDNA) sequences. These are copies of mRNA transcripts that correspond to the coding regions of genomes. Studying the expression patterns of genes is essential for understanding gene fun
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23

Strickson, Amanda J. "Analysis of expressed sequence tags mapping to the critical region of the 5q syndrome." Thesis, Open University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250497.

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24

Levick, Mark Philip. "Gene identification using expressed sequence tags : molecular characterisation of a protective Leishmania major peroxidoxin." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.625001.

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25

Das, Akan. "Generation and characterization of expressed sequence tags of tea (Camellia sinensis L (O) kuntze)." Thesis, University of North Bengal, 2011. http://hdl.handle.net/123456789/1469.

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Shah, Bindiya. "Identification of genes encoding secreted proteins of schistosomes." Thesis, University of York, 2000. http://etheses.whiterose.ac.uk/9801/.

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27

Mammadov, Jafar. "Towards Cloning the Leaf Rust Resistance Gene Rph5." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/28704.

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Leaf rust caused by Puccinia hordei is an important disease of barley (Hordeum vulgare) in many regions of the world. Yield losses up to 62% have been reported in susceptible cultivars. The Rph5 gene confers resistance to the most prevalent races (8 and 30) of barley leaf rust in the United States. Therefore, the molecular mapping of Rph5 is of great interest. Genetic studies were performed by analysis of 93 and 91 F2 plants derived from the crosses 'Bowman' (rph5) x 'Magnif 102' (Rph5) and 'Moore' (rph5) x Virginia 92-42-46 (Rph5), respectively. Linkage analysis positioned the Rph5 locus to t
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28

Douglas, Stephanie. "The development of molecular markers for use across all plant species using expressed sequence tags." FIU Digital Commons, 2006. http://digitalcommons.fiu.edu/etd/3234.

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There are over a half a million plant species on earth, and we use them in virtually every aspect of our lives. Little or no genomic information exists about the vast majority of these plants. This study investigated the use of Expressed Sequence Tags (ESTs) to locate highly conserved sequences from which to design a set of universal molecular markers for all plant species. Plant species for this study were chosen to representative of the plant kingdom. This was done by sampling several individuals of at least one species from all of the major terrestrial plant groups. Conserved sequences are
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29

O'Sullivan, Helen. "Association genetics in wheat and development of single nucleotide polymorphic markers from expressed sequence tags." Thesis, University of Bristol, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425140.

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30

Tran, Anh-Nhi. "A Genetic Survey of the Pathogenic Parasite Trypanosoma cruzi." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3425.

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31

Martins, Camila Augusta de Oliveira. "Seqüências ORESTES (open reading frame expressed sequence tags) de trypanosoma cruzi e transcrição de DNA satélite." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-19052008-135857/.

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Nos bancos de dados de T. cruzi há cerca de 3.750 ESTs de amastigotas e tripomastigotas, seqüenciadas a partir das extremidades 5\' ou 3\' de clones de cDNA. A metodologia ORESTES (Open Reading Frame Expressed Sequence Tags) possibilita obter seqüências transcritas parciais derivadas majoritariamente da porção central dos mRNAs, favorecendo a descoberta de novos genes. Neste trabalho, caracterizamos ORESTES de formas infectantes amastigotas e tripomastigotas da cepa humana VL10 (ATVL). A metodologia foi padronizada com formas epimastigotas da cepa CL Brener (ECL), monitorando
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Saripalli, Chandrasekhar. "ANNOTATION OF WHITEFLY EXPRESSED SEQUENCE TAGS AND VALIDATION OF GENES WITH POTENTIAL SIGNIFICANCE TO BEGOMOVIRUS TRANSMISSION." Thesis, The University of Arizona, 2008. http://hdl.handle.net/10150/193455.

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The whitefly Bemisia tabaci (Gennadius) (Hemiptera) complex is the sole arthropod vector of the genus, Begomovirus (family, Geminiviridae), which causes debilitating diseases of plants, worldwide. Virus-vector specificity is conferred through co-evolved, whitefly vector-viral capsid protein-protein interactions. Membrane-bound receptors are thought to facilitate virion passage across the gut-hemolymph and hemolymph-salivary gland interfaces, and virion circulation is expected to elicit innate defense and stress-related proteins. Our goal was to select and validate genes involved in whitefly-m
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Baudet, Christian. "Uma abordagem para detecção e remoção de artefatos em sequencias ESTs." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/276264.

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Orientador: Zanoni Dias<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Computação<br>Made available in DSpace on 2018-08-08T07:27:54Z (GMT). No. of bitstreams: 1 Baudet_Christian_M.pdf: 13612079 bytes, checksum: 648d18039dc13dcd5a2f422cc7863666 (MD5) Previous issue date: 2006<br>Resumo: O sequenciamento de ESTs (Expressed Sequence Tag) [2] e uma tecnica que trabalha com bibliotecas de cDNAs tendo como objetivo a obtençao de uma boa aproximaçao para o ?ndice genico, que e a listagem de genes existentes no genoma do organismo estudado. Antes da serem analisadas, as
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Nagai, Junichi. "Analysis of expressed sequence tags (ESTs) from female-sexual-organ cDNA library of a liverwort, Marchantia polymorpha." Kyoto University, 2000. http://hdl.handle.net/2433/181045.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第8416号<br>農博第1100号<br>新制||農||798(附属図書館)<br>学位論文||H12||N3373(農学部図書室)<br>UT51-2000-F320<br>京都大学大学院農学研究科農芸化学専攻<br>(主査)教授 大山 莞爾, 教授 關谷 次郎, 教授 佐藤 文彦<br>学位規則第4条第1項該当
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Weng, Huang Ju Lin. "Developing and using expressed sequence tags to study the predatory mite Phytoseiulus persimilis Athias-Henriot (Paraistiformes, Mesostigmata, Phytoseiidae)." Diss., Kansas State University, 2011. http://hdl.handle.net/2097/9193.

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Doctor of Philosophy<br>Department of Entomology<br>David C. Margolies<br>Yoonseong Park<br>The predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae) is one of the most frequently released natural enemies for biological control of spider mites in greenhouse and outdoors crops. In this research, I utilized Expresses Sequence Tags (ESTs), the most cost effective approach for transcriptome exploration, to study three different aspects of this arachnid species for which there is little genomic information. I combined two EST datasets from different whole body cDNA libraries and analyzed by
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Jaimes, Oscar O. "Construction and characterization of cDNA libraries bearing expressed sequence tags induced after treatment of Vicia faba leaves with saliylic acid." Berlin VWF, 2005. http://deposit.ddb.de/cgi-bin/dokserv?id=2709287&prov=M&dok_var=1&dok_ext=htm.

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Steller, Matthew Michael. "A comparative analysis of gene expression among castes of the termite Reticulitermes flavipes using expressed sequence tags (ESTs) and a microarray." Thesis, Manhattan, Kan. : Kansas State University, 2009. http://hdl.handle.net/2097/1471.

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Wamalwa, Mark. "Development of a comprehensive annotation and curation framework for analysis of Glossina Morsitans Morsitans expresses sequence tags." Thesis, University of the Western Cape, 2011. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_5497_1325767222.

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This study has successfully identified transcripts differentially expressed in the salivary gland and midgut and provides candidate genes that are critical to response to parasite invasion. Furthermore, an open-source Glossina resource (G-ESTMAP) was developed that provides interactive features and browsing of functional genomics data for researchers working in the field of Trypanosomiasis on the African continent.
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McManus, Andrew. "The identification of equine genes represented by expressed sequence tags and the subsequent characterisation of two immunologically relevant genes, RANTES and CD19." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627443.

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Bonaccorsi, Eric D\'Alessandro. "Regulação da expressão gênica por oxigênio em microrganismos eucariotos: análises de ESTs (Expressed Sequence Tags) e microrrays de cDNA de Trichoderma reesei." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-04052018-110230/.

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Glicose e oxigênio são moléculas essenciais para a maioria dos organismos vivos. Além de sua importância nos processos de produção de energia - glicose como fonte de carbono e energia e oxigênio como aceptor dos elétrons doados por NADH e FADH2 - estes dois compostos funcionam como efetuadores, modulando vários processos metabólicos e fisiológicos nas células. Visto que a mitocôndria é um dos alvos afetados pelas disponibilidades destas duas moléculas, nós isolamos e seqüenciamos o genoma mitocondrial de Trichoderma reesei, um fungo multicelular empregado neste trabalho como sistema modelo. Fo
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Garg, Kavita. "Genome-wide comparison of evolutionarily conserved alternative and constitutive splice sites /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10260.

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Geirardsdottir, Kristin. "Identifying and analysing alternative splice variants by aligning ESTs and mRNAs to the genomic sequence." Thesis, University of Skövde, School of Humanities and Informatics, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-963.

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<p>Questions have been raised about the genomic complexity of the human genome, since it was reported that it only consisted of 32,000 genes. Alternative splicing is considered the explanation of the enormous difference between the number of genes and the number of proteins. Aligning expressed sequence tags (ESTs) to the genomic sequence has become a popular approach for gene prediction, revealing alternative splice variants. The aim in this thesis is to identify and analyse splice variants of the adhesion family of G protein-coupled receptors using EST data. 75% of the genes in the data set o
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Sharanowski, Barbara J. "HYMENOPTERAN MOLECULAR PHYLOGENETICS: FROM APOCRITA TO BRACONIDAE (ICHNEUMONOIDEA)." Lexington, Ky. : [University of Kentucky Libraries], 2009. http://hdl.handle.net/10225/1013.

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Thesis (Ph. D.)--University of Kentucky, 2009.<br>Title from document title page (viewed on July 28, 2009). Document formatted into pages; contains: viii, 113 p. : ill. Includes abstract and vita. Includes bibliographical references (p. 101-111).
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Da, Silva Carla Cristina 1978. "Construction of a functional map for rubber tree (Hevea brasiliensis) = Construção de um mapa funcional em seringueira (Hevea brasiliensis)." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316470.

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Orientador: Anete Pereira de Souza.<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-25T14:31:52Z (GMT). No. of bitstreams: 1 DaSilva_CarlaCristina_D.pdf: 7310053 bytes, checksum: 1acc7f4e6eb7811b25670f85f9563217 (MD5) Previous issue date: 2014<br>Resumo: A seringueira (Hevea brasiliensis), espécie nativa da Amazônia, é a maior fonte de borracha natural do mundo. Programas de melhoramento genético da seringueira têm sido cruciais para a obtenção de caracteres desejáveis. Entretanto, o ciclo de melhoramento da seringueira é
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Desclés, Julien. "Phaeodactylum tricornutum : un nouveau modèle de diatomée pour l'étude du rôle du silicium dans le monde vivant ?" Paris 6, 2007. http://www.theses.fr/2007PA066070.

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Les diatomées sont des microalgues unicellulaires qui ont des rôles écologiques essentiels dans les cycles biogéochimiques du carbone et aussi du silicium, grâce à leur exosquelette en silice appelé frustule. Nous avons travaillé sur l’espèce modèle Phaeodactylum tricornutum, dont le génome est en cours d’annotation, et qui a la particularité de se présenter sous trois morphotypes différents : fusiforme, triradié et ovale. Seule la forme ovale peut synthétiser un exosquelette silicifié. Dans un premier temps, nous avons cherché à purifier par des méthodes biochimiques directes les partenaires
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Serres-Giardi, Laurana. "Diversité et évolution des paysages nucléotidiques des plantes." Thesis, Montpellier, SupAgro, 2012. http://www.theses.fr/2012NSAM0045/document.

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Le paysage nucléotidique – la manière dont la composition nucléotidique varie le long du génome – est une caractéristique marquante de l'organisation des génomes et varie fortement entre espèces. Plusieurs hypothèses émergent des nombreux débats autour des mécanismes évolutifs à l'origine de ces hétérogénéités du taux de GC, parmi lesquelles la conversion génique biaisée vers G et C (BGC) et la sélection sur l'usage du code (SUC). La BGC est un processus neutre associé à la recombinaison qui favorise les allèles en G ou C au détriment des allèles en A ou T. La SUC est une force de sélection qu
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Pedro, Uribe Mejia. "Dissection of defense responses of skl, an ethylene insensitive mutant of Medicago truncatula." Texas A&M University, 2004. http://hdl.handle.net/1969.1/1241.

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The interactions between Medicago truncatula and Phytophthora medicaginis were examined using skl, a mutant blocked in ethylene perception, and a range of wild accessions of this plant species. P. medicaginis infection of M. truncatula plants resulted in compatible responses, whereas the mutant genotype was found to be hyper-susceptible to the pathogen. Phytophthora reproduction and colonization rates of Medicago tissues supported this conclusion. Infection of skl with different pathogens reinforced this observation. Ethylene production in infected A17 and skl roots showed reduced ethylene e
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Burkhart, Tandace L. "The Search for Novel Sponge genes: Comparative Analysis of Gene Expression in Multiple Sponges." NSUWorks, 2012. http://nsuworks.nova.edu/occ_stuetd/194.

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This project focuses on the use of sponge genetic transcripts in the form of expressed sequence tags (ESTs) readily available in Genbank to search for novel genes using bioinformatics analysis tools. Marine sponge species are known to house a diversity of marine microbes and are known as the ‘living fossils’ of the animal kingdom because of the large number of ancient genes they house. Genomic mining can be a useful tool in discovering these orthologous genes. This study utilized the techniques of genomic mining of 11 previously described sponge species transcripts. The results of this study p
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Ferro, Milene. "Desenvolvimento e validação de protocolos para a anotação automática de sequências ORESTES de Eimeria spp. de galinha doméstica." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-31032009-114017/.

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A coccidiose aviária é uma doença entérica causada por protozoários parasitas do gênero Eimeria. Visando uma maior compreensão dos mecanismos moleculares envolvidos na regulação do ciclo de vida dos parasitas, foram geradas 15.000 seqüências expressas (ORESTES) para cada uma das três espécies mais importantes: E. tenella, E. maxima e E. acervulina. O presente trabalho consistiu no desenvolvimento de componentes de anotação automática de seqüências para o sistema EGene, plataforma previamente desenvolvida pelo nosso grupo (Durham et al. Bioinformatics 21: 2812-2813, 2005) para a construção de
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"EST expression and proteomic studies on mycelia of cordyceps militaris." 2004. http://library.cuhk.edu.hk/record=b5891998.

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Chan Ching-Man.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2004.<br>Includes bibliographical references (leaves 109-123).<br>Abstracts in English and Chinese.<br>Thesis committee --- p.i<br>Statement --- p.ii<br>Abstract --- p.iii<br>Acknowledgements --- p.vi<br>Abbreviations --- p.vii<br>Table of contents --- p.xi<br>List of figures --- p.xiv<br>List of tables --- p.xv<br>Chapter 1. --- Introduction --- p.1<br>Chapter 2. --- Literature review --- p.3<br>Chapter 2.1 --- History --- p.3<br>Chapter 2.2 --- The living environment and life cycles of Cordyccps --- p.3<br>Chap
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