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Journal articles on the topic 'Expression; Function; Histone-like protein H-NS'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Nishino, Kunihiko, and Akihito Yamaguchi. "Role of Histone-Like Protein H-NS in Multidrug Resistance of Escherichia coli." Journal of Bacteriology 186, no. 5 (2004): 1423–29. http://dx.doi.org/10.1128/jb.186.5.1423-1429.2004.

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ABSTRACT The histone-like protein H-NS is a major component of the bacterial nucleoid and plays a crucial role in global gene regulation of enteric bacteria. It is known that the expression of a variety of genes is repressed by H-NS, and mutations in hns result in various phenotypes, but the role of H-NS in the drug resistance of Escherichia coli has not been known. Here we present data showing that H-NS contributes to multidrug resistance by regulating the expression of multidrug exporter genes. Deletion of the hns gene from the ΔacrAB mutant increased levels of resistance against antibiotics

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2

Jian, Huahua, Guanpeng Xu, Yingbao Gai, Jun Xu, and Xiang Xiao. "The Histone-Like Nucleoid Structuring Protein (H-NS) Is a Negative Regulator of the Lateral Flagellar System in the Deep-Sea Bacterium Shewanella piezotolerans WP3." Applied and Environmental Microbiology 82, no. 8 (2016): 2388–98. http://dx.doi.org/10.1128/aem.00297-16.

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ABSTRACTAlthough the histone-like nucleoid structuring protein (H-NS) is well known for its involvement in the adaptation of mesophilic bacteria, such asEscherichia coli, to cold environments and high-pressure stress, an understanding of the role of H-NS in the cold-adapted benthic microorganisms that live in the deep-sea ecosystem, which covers approximately 60% of the earth's surface, is still lacking. In this study, we characterized the function of H-NS inShewanella piezotoleransWP3, which was isolated from West Pacific sediment at a depth of 1,914 m. Anhnsgene deletion mutant (WP3Δhns) was

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Kim, Eun A., and David F. Blair. "Function of the Histone-Like Protein H-NS in Motility of Escherichia coli: Multiple Regulatory Roles Rather than Direct Action at the Flagellar Motor." Journal of Bacteriology 197, no. 19 (2015): 3110–20. http://dx.doi.org/10.1128/jb.00309-15.

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ABSTRACTA number of investigations ofEscherichia colihave suggested that the DNA-binding protein H-NS, in addition to its well-known functions in chromosome organization and gene regulation, interacts directly with the flagellar motor to modulate its function. Here, in a study initially aimed at characterizing the H-NS/motor interaction further, we identify problems and limitations in the previous work that substantially weaken the case for a direct H-NS/motor interaction. Nullhnsmutants are immotile, largely owing to the downregulation of the flagellar master regulators FlhD and FlhC. We, and

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Paytubi, Sonia, Jesús García, and Antonio Juárez. "Bacterial Hha-like proteins facilitate incorporation of horizontally transferred DNA." Open Life Sciences 6, no. 6 (2011): 879–86. http://dx.doi.org/10.2478/s11535-011-0071-3.

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AbstractHorizontal gene transfer (HGT), non-hereditary transfer of genetic material between organisms, accounts for a significant proportion of the genetic variability in bacteria. In Gram negative bacteria, the nucleoid-associated protein H-NS silences unwanted expression of recently acquired foreign DNA. This, in turn, facilitates integration of the incoming genes into the regulatory networks of the recipient cell. Bacteria belonging to the family Enterobacteriaceae express an additional protein, the Hha protein that, by binding to H-NS, potentiates silencing of HGT DNA. We provide here an o

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5

Castang, Sandra, and Simon L. Dove. "Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa." Journal of Bacteriology 194, no. 18 (2012): 5101–9. http://dx.doi.org/10.1128/jb.00932-12.

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ABSTRACTMembers of the histone-like nucleoid-structuring (H-NS) family of proteins have been shown to play important roles in silencing gene expression and in nucleoid compaction. InPseudomonas aeruginosa, the two H-NS family members MvaT and MvaU are thought to bind the same AT-rich regions of the chromosome and function coordinately to control a common set of genes. Here we present evidence that the loss of both MvaT and MvaU cannot be tolerated because it results in the production of Pf4 phage that superinfect and kill cells or inhibit their growth. Using a ClpXP-based protein depletion sys

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Stratmann, Thomas, S. Madhusudan, and Karin Schnetz. "Regulation of the yjjQ-bglJ Operon, Encoding LuxR-Type Transcription Factors, and the Divergent yjjP Gene by H-NS and LeuO." Journal of Bacteriology 190, no. 3 (2007): 926–35. http://dx.doi.org/10.1128/jb.01447-07.

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ABSTRACT The yjjQ and bglJ genes encode LuxR-type transcription factors conserved in several enterobacterial species. YjjQ is a potential virulence factor in avian pathogenic Escherichia coli. BglJ counteracts the silencing of the bgl (β-glucoside) operon by H-NS in E. coli K-12. Here we show that yjjQ and bglJ form an operon carried by E. coli K-12, whose expression is repressed by the histone-like nucleoid structuring (H-NS) protein. The LysR-type transcription factor LeuO counteracts this repression. Furthermore, the yjjP gene, encoding a membrane protein of unknown function and located ups

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Forns, Núria, Rosa C. Baños, Carlos Balsalobre, Antonio Juárez, and Cristina Madrid. "Temperature-Dependent Conjugative Transfer of R27: Role of Chromosome- and Plasmid-Encoded Hha and H-NS Proteins." Journal of Bacteriology 187, no. 12 (2005): 3950–59. http://dx.doi.org/10.1128/jb.187.12.3950-3959.2005.

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ABSTRACT IncHI plasmids encode multiple-antibiotic resistance in Salmonella enterica serovar Typhi. These plasmids have been considered to play a relevant role in the persistence and reemergence of this microorganism. The IncHI1 plasmid R27, which can be considered the prototype of IncHI plasmids, is thermosensitive for transfer. Conjugation frequency is highest at low temperature (25 to 30°C), decreasing when temperature increases. R27 codifies an H-NS-like protein (open reading frame 164 [ORF164]) and an Hha-like protein (ORF182). The H-NS and Hha proteins participate in the thermoregulation

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8

Sonden, B., and B. E. Uhlin. "Coordinated and differential expression of histone-like proteins in Escherichia coli: regulation and function of the H-NS analog StpA." EMBO Journal 15, no. 18 (1996): 4970–80. http://dx.doi.org/10.1002/j.1460-2075.1996.tb00877.x.

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9

Wang, Yan, Yiquan Zhang, Zhe Yin, et al. "H-NS represses transcription of the flagellin gene lafA of lateral flagella in Vibrio parahaemolyticus." Canadian Journal of Microbiology 64, no. 1 (2018): 69–74. http://dx.doi.org/10.1139/cjm-2017-0315.

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Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf genes is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS-like protein-dependent expression of laf genes has been identified in Vibrio cholerae and V. parahaemolyticus. The data presented here show that H-NS acts as a repressor of the swarming motility in V. parahaemolyticus. A single σ28-depende

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10

Liu, Baomo, Lili Shui, Kai Zhou, et al. "Impact of Plasmid-Encoded H-NS–like Protein on blaNDM-1-Bearing IncX3 Plasmid in Escherichia coli." Journal of Infectious Diseases 221, Supplement_2 (2020): S229—S236. http://dx.doi.org/10.1093/infdis/jiz567.

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Abstract Background This study was performed to assess the role of the histone-like nucleoid-structuring (H-NS)–like protein, carried by blaNDM-1-encoding IncX3-type plasmids, in the dissemination of IncX3 plasmids. Methods The blaNDM-1-encoding IncX3 plasmids were analyzed using southern blot, conjugation, and competition assays. Virulence was evaluated with a Galleria mellonella infection model. An hns-knockout IncX3 plasmid was also constructed to identify the functions of plasmid-borne H-NS–like protein in Escherichia coli. Results The assasys detected blaNDM-1-encoding IncX3-type plasmids

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Wagner, Karin, Jennifer Schilling, Stefan Fälker, M. Alexander Schmidt, and Gerhard Heusipp. "A Regulatory Network Controls Expression of the In Vivo-Expressed HreP Protease of Yersinia enterocolitica." Journal of Bacteriology 191, no. 5 (2008): 1666–76. http://dx.doi.org/10.1128/jb.01517-08.

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ABSTRACT The human enteropathogen Yersinia enterocolitica survives and replicates in the lymphoid tissues of its host. Previous in vivo analyses of gene expression revealed that various chromosomal genes are expressed at this stage of infection, but not in vitro. One of these, termed hreP, encodes a protease that is necessary for full virulence of Y. enterocolitica. Using transposon mutagenesis, we identified three genes, pypA, pypB, and pypC, as positive regulators of hreP transcription. PypA is an inner membrane protein with no significant similarity to any known proteins; PypB is a ToxR-lik

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Ohta, Tomoko, Chiharu Ueguchi, and Takeshi Mizuno. "rpoS Function Is Essential forbgl Silencing Caused by C-Terminally Truncated H-NS inEscherichia coli." Journal of Bacteriology 181, no. 20 (1999): 6278–83. http://dx.doi.org/10.1128/jb.181.20.6278-6283.1999.

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ABSTRACT From evolutionary and physiological viewpoints, theEscherichia coli bgl operon is intriguing because its expression is silent (Bgl− phenotype), at least under several laboratory conditions. H-NS, a nucleoid protein, is known as a DNA-binding protein involved in bgl silencing. However, we previously found that bgl expression is still silent in a certain subset of hns mutations, each of which results in a defect in its DNA-binding ability. Based on this fact, we proposed a model in which a postulated DNA-binding protein(s) has an adapter function by interacting with both the cis-acting

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13

Easton, Donna M., Luke P. Allsopp, Minh-Duy Phan, et al. "The Intimin-Like Protein FdeC Is Regulated by H-NS and Temperature in Enterohemorrhagic Escherichia coli." Applied and Environmental Microbiology 80, no. 23 (2014): 7337–47. http://dx.doi.org/10.1128/aem.02114-14.

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ABSTRACTEnterohemorrhagicEscherichia coli(EHEC) is a Shiga-toxigenic pathogen capable of inducing severe forms of enteritis (e.g., hemorrhagic colitis) and extraintestinal sequelae (e.g., hemolytic-uremic syndrome). The molecular basis of colonization of human and animal hosts by EHEC is not yet completely understood, and an improved understanding of EHEC mucosal adherence may lead to the development of interventions that could disrupt host colonization. FdeC, also referred to by its IHE3034 locus tag ECOK1_0290, is an intimin-like protein that was recently shown to contribute to kidney coloni

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Lippa, Andrew M., Michael J. Gebhardt, and Simon L. Dove. "1192. H-NS-like Proteins in Pseudomonas aeruginosa Coordinately Silence Intragenic and Antisense Transcription." Open Forum Infectious Diseases 7, Supplement_1 (2020): S618—S619. http://dx.doi.org/10.1093/ofid/ofaa439.1377.

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Abstract Background The H-NS-like proteins MvaT and MvaU act coordinately as global repressors in Pseudomonas aeruginosa by binding to AT-rich regions of the chromosome, which include horizontally acquired genes and numerous virulence factors. Although cells can tolerate the loss of either protein, identifying their combined regulatory effects has been challenging because the loss of both proteins is lethal due to induction of the prophage Pf4 and subsequent superinfection of the cell. Although in other bacteria, H-NS promotes cellular fitness by inhibiting intragenic transcription from AT-ric

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Qu, Xueqi, Christiane Neuhoff, Mehmet Ulas Cinar, et al. "Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells." Biology 10, no. 6 (2021): 490. http://dx.doi.org/10.3390/biology10060490.

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Inflammation is regulated by epigenetic modifications, including DNA methylation and histone acetylation. Sulforaphane (SFN), a histone deacetylase (HDAC) inhibitor, is also a potent immunomodulatory agent, but its anti-inflammatory functions through epigenetic modifications remain unclear. Therefore, this study aimed to investigate the epigenetic effects of SFN in maintaining the immunomodulatory homeostasis of innate immunity during acute inflammation. For this purpose, SFN-induced epigenetic changes and expression levels of immune-related genes in response to lipopolysaccharide (LPS) stimul

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Caballero-Flores, Gustavo G., Matthew A. Croxen, Verónica I. Martínez-Santos, B. Brett Finlay, and José L. Puente. "Identification and Regulation of a Novel Citrobacter rodentium Gut Colonization Fimbria (Gcf)." Journal of Bacteriology 197, no. 8 (2015): 1478–91. http://dx.doi.org/10.1128/jb.02486-14.

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ABSTRACTThe Gram-negative enteric bacteriumCitrobacter rodentiumis a natural mouse pathogen that has been extensively used as a surrogate model for studying the human pathogens enteropathogenic and enterohemorrhagicEscherichia coli. All three pathogens produce similar attaching and effacing (A/E) lesions in the intestinal epithelium. During infection, these bacteria employ surface structures called fimbriae to adhere and colonize the host intestinal epithelium. ForC. rodentium, the roles of only a small number of its genome-carried fimbrial operons have been evaluated. Here, we report the iden

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Grainger, David C. "Structure and function of bacterial H-NS protein." Biochemical Society Transactions 44, no. 6 (2016): 1561–69. http://dx.doi.org/10.1042/bst20160190.

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The histone-like nucleoid structuring (H-NS) protein is a major component of the folded chromosome in Escherichia coli and related bacteria. Functions attributed to H-NS include management of genome evolution, DNA condensation, and transcription. The wide-ranging influence of H-NS is remarkable given the simplicity of the protein, a small peptide, possessing rudimentary determinants for self-association, hetero-oligomerisation and DNA binding. In this review, I will discuss our understanding of H-NS with a focus on these structural elements. In particular, I will consider how these interaction

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Sun, Yaping, Elizabeth Weisiger, Tomomi Toubai, Charles Dinarello, James L. Ferrara, and Pavan Reddy. "A Critical Role for STAT-3 Dependent Induction of Indoleamine 2, 3 Dioxygenase in Histone Deacetylase Inhibitors Mediated the Regulation of GVHD." Blood 110, no. 11 (2007): 351. http://dx.doi.org/10.1182/blood.v110.11.351.351.

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Abstract Histone deacetylase (HDAC) inhibitors reduce experimental acute graft-versus-host disease (GVHD) and recent work by us and others suggest that HDAC inhibitors regulate dendritic cell (DC) function. However, the critical cellular and molecular mechanisms underpinning these observations are not known. We investigated the mechanisms by utilizing two HDAC inhibitors, suberoylanilide hydroxamic acid (SAHA) and ITF 2357. Pretreatment of murine bone marrow (BM) and human peripheral blood mononuclear cell derived DCs with either HDAC inhibtors and stimulated with TLR ligands such as LPS cause

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Madan, P., W. A. King, and D. H. Betts. "91 INHIBITION OF TELOMERASE REVERSE TRANSCRIPTASE (TERT) INDUCES EMBRYO ARREST IN BOVINE PRE-IMPLANTATION EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 146. http://dx.doi.org/10.1071/rdv21n1ab91.

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Previous studies from our laboratory have detected telomerase activity in embryos from all stages of early bovine development. However, the regulation of the telomerase subunits remains poorly understood. The objective of this study was to characterize the expression and function of the bovine telomerase reverse transcriptase (bTERT) subunit during bovine pre-implantation embryogenesis. Using RT-PCR and immunofluorescence staining procedures (n = 20 embryos at timed stages of development; r = 3), we demonstrate that mRNA transcripts and protein for bTERT were detected in pre-implantation bovin

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Hulton, Christopher S. J., Alexander Seirafi, Jay C. D. Hinton, et al. "Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria." Cell 63, no. 3 (1990): 631–42. http://dx.doi.org/10.1016/0092-8674(90)90458-q.

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Duysak, Taner, Thanh Tuyen Tran, Aqeel Rana Afzal, and Che-Hun Jung. "Fluorescence Spectroscopic Analysis of ppGpp Binding to cAMP Receptor Protein and Histone-Like Nucleoid Structuring Protein." International Journal of Molecular Sciences 22, no. 15 (2021): 7871. http://dx.doi.org/10.3390/ijms22157871.

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The cyclic AMP receptor protein (CRP) is one of the best-known transcription factors, regulating about 400 genes. The histone-like nucleoid structuring protein (H-NS) is one of the nucleoid-forming proteins and is responsible for DNA packaging and gene repression in prokaryotes. In this study, the binding of ppGpp to CRP and H-NS was determined by fluorescence spectroscopy. CRP from Escherichia coli exhibited intrinsic fluorescence at 341 nm when excited at 280 nm. The fluorescence intensity decreased in the presence of ppGpp. The dissociation constant of 35 ± 3 µM suggests that ppGpp binds to

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Ito, K., T. Oshima, T. Mizuno, and Y. Nakamura. "Regulation of lysyl-tRNA synthetase expression by histone-like protein H-NS of Escherichia coli." Journal of Bacteriology 176, no. 23 (1994): 7383–86. http://dx.doi.org/10.1128/jb.176.23.7383-7386.1994.

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Silva, Anisia J., Syed Zafar Sultan, Weili Liang, and Jorge A. Benitez. "Role of the Histone-Like Nucleoid Structuring Protein in the Regulation of rpoS and RpoS-Dependent Genes in Vibrio cholerae." Journal of Bacteriology 190, no. 22 (2008): 7335–45. http://dx.doi.org/10.1128/jb.00360-08.

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ABSTRACT Production of the Zn-metalloprotease hemagglutinin (HA)/protease by Vibrio cholerae has been reported to enhance enterotoxicity in rabbit ileal loops and the reactogenicity of live cholera vaccine candidates. Expression of HA/protease requires the alternate sigma factor σS (RpoS), encoded by rpoS. The histone-like nucleoid structuring protein (H-NS) has been shown to repress rpoS expression in Escherichia coli. In V. cholerae strains of the classical biotype, H-NS has been reported to silence virulence gene expression. In this study we examined the role of H-NS in the expression of HA

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Müller, Claudia M., Ulrich Dobrindt, Gábor Nagy, Levente Emödy, Bernt Eric Uhlin, and Jörg Hacker. "Role of Histone-Like Proteins H-NS and StpA in Expression of Virulence Determinants of Uropathogenic Escherichia coli." Journal of Bacteriology 188, no. 15 (2006): 5428–38. http://dx.doi.org/10.1128/jb.01956-05.

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ABSTRACT The histone-like protein H-NS is a global regulator in Escherichia coli that has been intensively studied in nonpathogenic strains. However, no comprehensive study on the role of H-NS and its paralogue, StpA, in gene expression in pathogenic E. coli has been carried out so far. Here, we monitored the global effects of H-NS and StpA in a uropathogenic E. coli isolate by using DNA arrays. Expression profiling revealed that more than 500 genes were affected by an hns mutation, whereas no effect of StpA alone was observed. An hns stpA double mutant showed a distinct gene expression patter

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Mertens, Daniel, Angela Philippen, Nupur Bhattacharya, et al. "Epimutation of the Tumor Suppressor Mechanism in 13q14.3 Involves Monoallelic Expression, Non-Coding RNA Genes and Deregulation of NFkB Signalling." Blood 112, no. 11 (2008): 783. http://dx.doi.org/10.1182/blood.v112.11.783.783.

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Abstract Deletions in chromosomal band 13q14.3 occur in a variety of human neoplasms like chronic lymphocytic leukaemia (CLL), indicating a tumor suppressor mechanism (TSM) in this region. Intriguingly, several characteristics of the region of interest point to an epigenetic pathomechanism: candidate protein-coding genes and non-coding RNA genes including miR15a and miR16-1 lack point mutations in the majority of patients, yet these genes are significantly downregulated in almost all CLL patients the presence of large non-coding RNA genes in 13q14.3 is reminiscent of imprinted regions where on

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Tippner, Detlev, Henning Afflerbach, Christiane Bradaczek, and Rolf Wagner. "Evidence for a regulatory function of the histone-like Escherichia coli protein H-NS in ribosomal RNA synthesis." Molecular Microbiology 11, no. 3 (1994): 589–604. http://dx.doi.org/10.1111/j.1365-2958.1994.tb00339.x.

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Franzon, J. H., and D. S. Santos. "A role for histone-like protein H1 (H-NS) in the regulation of hemolysin expression by Serratia marcescens." Brazilian Journal of Medical and Biological Research 37, no. 12 (2004): 1763–69. http://dx.doi.org/10.1590/s0100-879x2004001200001.

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Shiga, Yasuyuki, Yasuhiko Sekine, Yasunobu Kano, and Eiichi Ohtsubo. "Involvement of H-NS in Transpositional Recombination Mediated by IS1." Journal of Bacteriology 183, no. 8 (2001): 2476–84. http://dx.doi.org/10.1128/jb.183.8.2476-2484.2001.

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ABSTRACT IS1, the smallest active transposable element in bacteria, encodes a transposase that promotes inter- and intramolecular transposition. Host-encoded factors, e.g., histone-like proteins HU and integration host factor (IHF), are involved in the transposition reactions of some bacterial transposable elements. Host factors involved in the IS1 transposition reaction, however, are not known. We show that a plasmid with an IS1 derivative that efficiently produces transposase did not generate miniplasmids, the products of intramolecular transposition, in mutants deficient in a nucleoid-assoc

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Ono, Shusuke, Martin D. Goldberg, Tjelvar Olsson, Diego Esposito, Jay C. D. Hinton, and John E. Ladbury. "H-NS is a part of a thermally controlled mechanism for bacterial gene regulation." Biochemical Journal 391, no. 2 (2005): 203–13. http://dx.doi.org/10.1042/bj20050453.

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Temperature is a primary environmental stress to which micro-organisms must be able to adapt and respond rapidly. Whereas some bacteria are restricted to specific niches and have limited abilities to survive changes in their environment, others, such as members of the Enterobacteriaceae, can withstand wide fluctuations in temperature. In addition to regulating cellular physiology, pathogenic bacteria use temperature as a cue for activating virulence gene expression. This work confirms that the nucleoid-associated protein H-NS (histone-like nucleoid structuring protein) is an essential componen

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Coker, Christopher, Olubunmi O. Bakare, and Harry L. T. Mobley. "H-NS Is a Repressor of the Proteus mirabilis Urease Transcriptional Activator GeneureR." Journal of Bacteriology 182, no. 9 (2000): 2649–53. http://dx.doi.org/10.1128/jb.182.9.2649-2653.2000.

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ABSTRACT Expression of Proteus mirabilis urease is governed by UreR, an AraC-like positive transcriptional activator. A poly(A) tract nucleotide sequence, consisting of A6TA2CA2TGGTA5GA6TGA5, is located 16 bp upstream of the ς70-likeureR promoter P2. Since poly(A) tracts of DNA serve as binding sites for the gene repressor histone-like nucleoid structuring protein (H-NS), we measured β-galactosidase activity of wild-typeEscherichia coli MC4100 (H-NS+) and its isogenic derivative ATM121 (hns::Tn10) (H-NS−) harboring a ureR-lacZ operon fusion plasmid (pLC9801). β-Galactosidase activity in the H-

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Eijkelkamp, Bart A., Uwe H. Stroeher, Karl A. Hassan, Liam D. H. Elbourne, Ian T. Paulsen, and Melissa H. Brown. "H-NS Plays a Role in Expression of Acinetobacter baumannii Virulence Features." Infection and Immunity 81, no. 7 (2013): 2574–83. http://dx.doi.org/10.1128/iai.00065-13.

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ABSTRACTAcinetobacter baumanniihas become a major problem in the clinical setting with the prevalence of infections caused by multidrug-resistant strains on the increase. Nevertheless, only a limited number of molecular mechanisms involved in the success ofA. baumanniias a human pathogen have been described. In this study, we examined the virulence features of a hypermotile derivative ofA. baumanniistrain ATCC 17978, which was found to display enhanced adherence to human pneumocytes and elevated levels of lethality towardCaenorhabditis elegansnematodes. Analysis of cellular lipids revealed mod

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Park, Kwon-Sam, Michiko Arita, Tetsuya Iida, and Takeshi Honda. "vpaH, a Gene Encoding a Novel Histone-Like Nucleoid Structure-Like Protein That Was Possibly Horizontally Acquired, Regulates the Biogenesis of Lateral Flagella in trh-Positive Vibrio parahaemolyticus TH3996." Infection and Immunity 73, no. 9 (2005): 5754–61. http://dx.doi.org/10.1128/iai.73.9.5754-5761.2005.

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ABSTRACT A histone-like nucleoid structure (H-NS) is a major component of the bacterial nucleoid and plays a crucial role in the global gene regulation of enteric bacteria. Here, we cloned and characterized the gene for the H-NS-like protein VpaH in Vibrio parahaemolyticus. vpaH encodes a protein of 134 amino acids that shows approximately 55%, 54%, and 41% identities with VicH in Vibrio cholerae, H-NS in V. parahaemolyticus, and H-NS in Escherichia coli, respectively. The vpaH gene was found in only trh-positive V. parahaemolyticus strains and not in Kanagawa-positive or in trh-negative envir

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Yun, Choong-Soo, Chiho Suzuki, Kunihiko Naito, et al. "Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded by the IncP-7 Plasmid pCAR1, Is a Key Global Regulator That Alters Host Function." Journal of Bacteriology 192, no. 18 (2010): 4720–31. http://dx.doi.org/10.1128/jb.00591-10.

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ABSTRACT Histone-like protein H1 (H-NS) family proteins are nucleoid-associated proteins (NAPs) conserved among many bacterial species. The IncP-7 plasmid pCAR1 is transmissible among various Pseudomonas strains and carries a gene encoding the H-NS family protein, Pmr. Pseudomonas putida KT2440 is a host of pCAR1, which harbors five genes encoding the H-NS family proteins PP_1366 (TurA), PP_3765 (TurB), PP_0017 (TurC), PP_3693 (TurD), and PP_2947 (TurE). Quantitative reverse transcription-PCR (qRT-PCR) demonstrated that the presence of pCAR1 does not affect the transcription of these five gene

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Gowrishankar, J., and A. J. Pittard. "Superimposition of TyrR Protein-Mediated Regulation on Osmoresponsive Transcription of Escherichia coli proUIn Vivo." Journal of Bacteriology 180, no. 24 (1998): 6743–48. http://dx.doi.org/10.1128/jb.180.24.6743-6748.1998.

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ABSTRACT Osmotic regulation of proU expression in the enterobacteria is achieved, at least in part, by a repression mechanism involving the histone-like nucleoid protein H-NS. By the creation of binding sites for the TyrR regulator protein in the vicinity of the ς70-controlled promoter of proU inEscherichia coli, we were able to demonstrate a superposed TyrR-mediated activation by l-phenylalanine (Phe), as well as repression by l-tyrosine, of proU expression in vivo. Based on the facts that pronounced activation in the presence of Phe was observed even at a low osmolarity and that the affinity

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Cabello, Olga A., Elena Eliseeva, WeiGong He, et al. "Cell Cycle-dependent Expression and Nucleolar Localization of hCAP-H." Molecular Biology of the Cell 12, no. 11 (2001): 3527–37. http://dx.doi.org/10.1091/mbc.12.11.3527.

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Condensin is a conserved 13S heteropentamer composed of two nonidentical structural maintenance of chromosome (SMC) family proteins, in Xenopus XCAP-C and XCAP-E, and three regulatory subunits, XCAP-D2, XCAP-G, and XCAP-H. Both biochemical and genetic analyses have demonstrated an essential role for the 13S condensin complex in mitotic chromosome condensation. Further, a potential requirement for condensin in completion of chromatid arm separation in early anaphase is demonstrated by the mutational phenotypes of the Drosophila homologues ofXCAP-H, barren and XCAP-C,DmSMC4. In this study we hav

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Japaridze, Aleksandre, Sylvain Renevey, Patrick Sobetzko, et al. "Spatial organization of DNA sequences directs the assembly of bacterial chromatin by a nucleoid-associated protein." Journal of Biological Chemistry 292, no. 18 (2017): 7607–18. http://dx.doi.org/10.1074/jbc.m117.780239.

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Structural differentiation of bacterial chromatin depends on cooperative binding of abundant nucleoid-associated proteins at numerous genomic DNA sites and stabilization of distinct long-range nucleoprotein structures. Histone-like nucleoid-structuring protein (H-NS) is an abundant DNA-bridging, nucleoid-associated protein that binds to an AT-rich conserved DNA sequence motif and regulates both the shape and the genetic expression of the bacterial chromosome. Although there is ample evidence that the mode of H-NS binding depends on environmental conditions, the role of the spatial organization

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Feng, Jia-Xun, Zhi-Zhong Song, Cheng-Jie Duan, et al. "The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice." Microbiology 155, no. 9 (2009): 3033–44. http://dx.doi.org/10.1099/mic.0.028910-0.

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Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice, one of the most serious rice diseases. The xrvA gene from Xoo strain 13751 encodes a protein containing a histone-like nucleoid-structuring protein (H-NS) domain. The expression of xrvA in strain 13751 was enhanced in XOM2 minimal medium. Mutation of the xrvA gene of strain 13751 led to a significant reduction in virulence in the host plant rice, a delayed hypersensitive response in the nonhost castor-oil plant, a decrease in extracellular polysaccharide and diffusible signal factor production, and an increase in intr

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Salomon, Dor, John A. Klimko, and Kim Orth. "H-NS regulates the Vibrio parahaemolyticus type VI secretion system 1." Microbiology 160, no. 9 (2014): 1867–73. http://dx.doi.org/10.1099/mic.0.080028-0.

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The marine bacterium Vibrio parahaemolyticus, a major cause of food-borne gastroenteritis, employs a type VI secretion system 1 (T6SS1), a recently discovered protein secretion system, to combat competing bacteria. Environmental signals such as temperature, salinity, cell density and surface sensing, as well as the quorum-sensing master regulator OpaR, were previously reported to regulate T6SS1 activity and expression. In this work, we set out to identify additional transcription regulators that control the tightly regulated T6SS1 activity. To this end, we determined the effect of deletions in

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Ricci, Dante P., Michael D. Melfi, Keren Lasker, David L. Dill, Harley H. McAdams, and Lucy Shapiro. "Cell cycle progression inCaulobacterrequires a nucleoid-associated protein with high AT sequence recognition." Proceedings of the National Academy of Sciences 113, no. 40 (2016): E5952—E5961. http://dx.doi.org/10.1073/pnas.1612579113.

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Faithful cell cycle progression in the dimorphic bacteriumCaulobacter crescentusrequires spatiotemporal regulation of gene expression and cell pole differentiation. We discovered an essential DNA-associated protein, GapR, that is required forCaulobactergrowth and asymmetric division. GapR interacts with adenine and thymine (AT)-rich chromosomal loci, associates with the promoter regions of cell cycle-regulated genes, and shares hundreds of recognition sites in common with known master regulators of cell cycle-dependent gene expression. GapR target loci are especially enriched in binding sites

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Brunet, Yannick R., Ahmad Khodr, Laureen Logger, et al. "H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing." Infection and Immunity 83, no. 7 (2015): 2738–50. http://dx.doi.org/10.1128/iai.00198-15.

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The secretion of bacterial toxin proteins is achieved by dedicated machineries called secretion systems. The type VI secretion system (T6SS) is a widespread versatile machine used for the delivery of protein toxins to both prokaryotic and eukaryotic cells. InSalmonella entericaserovar Typhimurium, the expression of the T6SS genes is activated during macrophage or mouse infection. Here, we show that the T6SS gene cluster is silenced by the histone-like nucleoid structuring H-NS protein using a combination of reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluore

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Soutourina, O., A. Kolb, E. Krin, et al. "Multiple Control of Flagellum Biosynthesis in Escherichia coli: Role of H-NS Protein and the Cyclic AMP-Catabolite Activator Protein Complex in Transcription of the flhDC Master Operon." Journal of Bacteriology 181, no. 24 (1999): 7500–7508. http://dx.doi.org/10.1128/jb.181.24.7500-7508.1999.

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ABSTRACT Little is known about the molecular mechanism by which histone-like nucleoid-structuring (H-NS) protein and cyclic AMP-catabolite activator protein (CAP) complex control bacterial motility. In the present paper, we show that crp and hns mutants are nonmotile due to a complete lack of flagellin accumulation. This results from a reduced expression in vivo of fliA and fliC, which encode the specific flagellar sigma factor and flagellin, respectively. Overexpression of the flhDC master operon restored, at least in part, motility in crp andhns mutant strains, suggesting that this operon is

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Hekmat, Azadeh, Mojtaba Sadeghi Manesh, Zahra Hajebrahimi, and Shadie Hatamie. "Microgravity-Induced Alterations in the H3.3B (H3F3B) Gene Expression and the Histone H3 Structure." Advanced Science, Engineering and Medicine 12, no. 8 (2020): 1084–94. http://dx.doi.org/10.1166/asem.2020.2672.

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It has been believed that microgravity directly can modify the structure, function, and morphology of biosystems and numerous researches have been performed to recognize these alterations. Since histone H3 is an essential protein in the field of epigenetics, this research aimed to evaluate the effects of simulated microgravity on the human H3.3B (H3F3B) gene expression and histone H3 structure. The two-dimensional clinostat was applied for simulating microgravity. Analysis of the gene expression by real-time quantitative PCR revealed that simulated microgravity diminished the expression level

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Erdő-Bonyár, Szabina, Judit Rapp, Tünde Minier, et al. "Toll-Like Receptor Mediated Activation of Natural Autoantibody Producing B Cell Subpopulations in an Autoimmune Disease Model." International Journal of Molecular Sciences 20, no. 24 (2019): 6152. http://dx.doi.org/10.3390/ijms20246152.

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Altered expression and function of the Toll-like receptor (TLR) homologue CD180 molecule in B cells have been associated with autoimmune disorders. In this study, we report decreased expression of CD180 at protein and mRNA levels in peripheral blood B cells of diffuse cutaneous systemic sclerosis (dcSSc) patients. To analyze the effect of CD180 stimulation, together with CpG (TLR9 ligand) treatment, on the phenotype defined by CD19/CD27/IgD/CD24/CD38 staining, and function (CD69 and CD180 expression, cytokine and antibody secretion) of B cell subpopulations, we used tonsillar B cells. After st

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Martínez-Santos, Verónica I., Abraham Medrano-López, Zeus Saldaña, Jorge A. Girón, and José L. Puente. "Transcriptional Regulation of theecpOperon by EcpR, IHF, and H-NS in Attaching and Effacing Escherichia coli." Journal of Bacteriology 194, no. 18 (2012): 5020–33. http://dx.doi.org/10.1128/jb.00915-12.

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ABSTRACTEnteropathogenic (EPEC) and enterohemorrhagic (EHEC)Escherichia coliare clinically important diarrheagenic pathogens that adhere to the intestinal epithelial surface. TheE. colicommon pili (ECP), or meningitis-associated and temperature-regulated (MAT) fimbriae, are ubiquitous among both commensal and pathogenicE. colistrains and play a role as colonization factors by promoting the interaction between bacteria and host epithelial cells and favoring interbacterial interactions in biofilm communities. The first gene of theecpoperon encodes EcpR (also known as MatA), a proposed regulatory

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An, Beum-Soo, Luz E. Tavera-Mendoza, Vassil Dimitrov, et al. "Stimulation of Sirt1-Regulated FoxO Protein Function by the Ligand-Bound Vitamin D Receptor." Molecular and Cellular Biology 30, no. 20 (2010): 4890–900. http://dx.doi.org/10.1128/mcb.00180-10.

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ABSTRACT Hormonal vitamin D, 1,25-dihydroxyvitamin D (1,25D), signals through the nuclear vitamin D receptor (VDR). 1,25D regulates cell proliferation and differentiation and has been identified as a cancer chemopreventive agent. FoxO proteins are transcription factors that control cell proliferation and survival. They function as tumor suppressors and are associated with longevity in several organisms. Accumulating data have revealed that 1,25D and FoxO proteins regulate similarly common target genes. We show here that the ligand-bound VDR regulates the posttranslational modification and func

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Chatterjee, Arpita, Pradeep K. Dutta, and Rukhsana Chowdhury. "Effect of Fatty Acids and Cholesterol Present in Bile on Expression of Virulence Factors and Motility of Vibrio cholerae." Infection and Immunity 75, no. 4 (2007): 1946–53. http://dx.doi.org/10.1128/iai.01435-06.

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ABSTRACT Bile induces pleiotropic responses that affect production of virulence factors, motility, and other phenotypes in the enteric pathogen Vibrio cholerae. Since bile is a heterogeneous mixture, crude bile was fractionated, and the components that mediate virulence gene repression and enhancement of motility were identified by nuclear magnetic resonance, gas chromatography (GC), and GC-mass spectrometry analyses. The unsaturated fatty acids detected in bile, arachidonic, linoleic, and oleic acids, drastically repressed expression of the ctxAB and tcpA genes, which encode cholera toxin and

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Choi, Garam, Kyung Ku Jang, Jong Gyu Lim, Zee-Won Lee, Hanhyeok Im, and Sang Ho Choi. "The transcriptional regulator IscR integrates host-derived nitrosative stress and iron starvation in activation of the vvhBA operon in Vibrio vulnificus." Journal of Biological Chemistry 295, no. 16 (2020): 5350–61. http://dx.doi.org/10.1074/jbc.ra120.012724.

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For successful infection of their hosts, pathogenic bacteria recognize host-derived signals that induce the expression of virulence factors in a spatiotemporal manner. The fulminating food-borne pathogen Vibrio vulnificus produces a cytolysin/hemolysin protein encoded by the vvhBA operon, which is a virulence factor preferentially expressed upon exposure to murine blood and macrophages. The Fe-S cluster containing transcriptional regulator IscR activates the vvhBA operon in response to nitrosative stress and iron starvation, during which the cellular IscR protein level increases. Here, electro

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Naz, R. K., K. Ahmad, and P. Kaplan. "Expression and function of ras proto-oncogene proteins in human sperm cells." Journal of Cell Science 102, no. 3 (1992): 487–94. http://dx.doi.org/10.1242/jcs.102.3.487.

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The presence and role of c-ras proteins were investigated in mature human sperm cells. The v-H-ras monoclonal antibody (mAb) against the c-ras protein, p21, reacted specifically with the acrosomal region of methanol-fixed as well as unfixed-live capacitated and non-capacitated human sperm cell in the indirect immunofluorescence technique. The v-H-ras mAb predominantly recognized c-ras protein of 21 kDa on the Western blot of lithium diiodosalicylate (LIS)-solubilized human sperm preparation. The incubation of sperm cells with v-H-ras mAb affected the sperm cell function in the human sperm pene

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Atlung, T., S. Sund, K. Olesen, and L. Brøndsted. "The histone-like protein H-NS acts as a transcriptional repressor for expression of the anaerobic and growth phase activator AppY of Escherichia coli." Journal of bacteriology 178, no. 12 (1996): 3418–25. http://dx.doi.org/10.1128/jb.178.12.3418-3425.1996.

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Purtov, Yuri A., Olga A. Glazunova, Sergey S. Antipov, et al. "Promoter islandsas a platform for interaction with nucleoid proteins and transcription factors." Journal of Bioinformatics and Computational Biology 12, no. 02 (2014): 1441006. http://dx.doi.org/10.1142/s0219720014410066.

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Seventy-eight promoter islands with an extraordinarily high density of potential promoters have been recently found in the genome of Escherichia coli. It has been shown that RNA polymerase binds internal promoters of these islands and produces short oligonucleotides, while the synthesis of normal mRNAs is suppressed. This quenching may be biologically relevant, as most islands are associated with foreign genes, which expression may deplete cellular resources. However, a molecular mechanism of silencing with the participation of these promoter-rich regions remains obscure. It has been demonstra

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