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Relevant bibliographies by topics / Expression plasmid

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Academic literature on the topic 'Expression plasmid'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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Journal articles on the topic "Expression plasmid"

1

Mitchell-Felton, Heather, and Susan C. Kandarian. "Normalization of muscle plasmid uptake by Southern blot: application to SERCA1 promoter analysis." American Journal of Physiology-Cell Physiology 277, no. 6 (1999): C1269—C1276. http://dx.doi.org/10.1152/ajpcell.1999.277.6.c1269.

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Direct injection of plasmid DNA into muscle allows the study of promoters in a physiological environment. Because of the variability of reporter gene activity, attempts have been made to normalize activity to muscle plasmid uptake by coinjection of a second “control” plasmid whose reporter gene is constitutively expressed by a viral promoter. The purpose of this study was to evaluate the use of a control plasmid vs. Southern blot to normalize for differences in uptake of plasmids containing promoter fragments of the skeletal muscle-specific sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1) gen

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Clark, Leann, Isabel Martinez-Argudo, Tom J. Humphrey, and Mark A. Jepson. "GFP plasmid-induced defects in Salmonella invasion depend on plasmid architecture, not protein expression." Microbiology 155, no. 2 (2009): 461–67. http://dx.doi.org/10.1099/mic.0.025700-0.

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We have investigated the impact of plasmids and GFP expression on invasion of cultured epithelial cells by Salmonella enterica Typhimurium strain SL1344. The invasiveness of SL1344 carrying plasmids derived from pBR322, encoding promoterless GFP or constitutively expressed rpsM-GFP, was compared under optimal growth conditions with that of SL1344(pBR322), unmodified SL1344 and a strain with chromosome-integrated rpsM-GFP. The strain carrying pBR322 exhibited normal invasion, but the presence of modified plasmids impaired invasiveness, and impairment was exacerbated by plasmid-encoded chloramph

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Galen, James E., Jay Nair, Jin Yuang Wang, et al. "Optimization of Plasmid Maintenance in the Attenuated Live Vector Vaccine Strain Salmonella typhiCVD 908-htrA." Infection and Immunity 67, no. 12 (1999): 6424–33. http://dx.doi.org/10.1128/iai.67.12.6424-6433.1999.

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ABSTRACT The broad objective of the research presented here is to develop a noncatalytic plasmid maintenance system for the stabilization of multicopy expression plasmids encoding foreign antigens in aSalmonella typhi live-vector vaccine strain such as CVD 908-htrA. We have enhanced the maintenance of expression plasmids at two independent levels. First, we removed dependence upon balanced-lethal maintenance systems that involve catalytic enzymes expressed from multicopy plasmids; we accomplished this through incorporation into expression plasmids of a postsegregational killing system based on

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Harth, Günter, Saša Masleša-Galić, and Marcus A. Horwitz. "A two-plasmid system for stable, selective-pressure-independent expression of multiple extracellular proteins in mycobacteria." Microbiology 150, no. 7 (2004): 2143–51. http://dx.doi.org/10.1099/mic.0.27113-0.

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Recombinant mycobacteria expressing Mycobacterium tuberculosis extracellular proteins are leading candidates for new vaccines against tuberculosis and other mycobacterial diseases, and important tools both in antimycobacterial drug development and basic research in mycobacterial pathogenesis. Recombinant mycobacteria that stably overexpress and secrete major extracellular proteins of M. tuberculosis in native form on plasmids pSMT3 and pNBV1 were previously constructed by the authors. To enhance the versatility of this plasmid-based approach for mycobacterial protein expression, the Escherichi

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Nakashima, Nobutaka, and Tomohiro Tamura. "Isolation and Characterization of a Rolling-Circle-Type Plasmid from Rhodococcus erythropolis and Application of the Plasmid to Multiple-Recombinant-Protein Expression." Applied and Environmental Microbiology 70, no. 9 (2004): 5557–68. http://dx.doi.org/10.1128/aem.70.9.5557-5568.2004.

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ABSTRACT We isolated, sequenced, and characterized the cryptic plasmid pRE8424 from Rhodococcus erythropolis DSM8424. Plasmid pRE8424 is a 5,987-bp circular plasmid; it carries six open reading frames and also contains cis-acting elements, specifically a single-stranded origin and a double-stranded origin, which are characteristic of rolling-circle-replication plasmids. Experiments with pRE8424 derivatives carrying a mutated single-stranded origin sequence showed that single-stranded DNA intermediates accumulated in the cells because of inefficient conversion from single-stranded DNA to double

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Hingston, Patricia, Thomas Brenner, Lisbeth Truelstrup Hansen, and Siyun Wang. "Comparative Analysis of Listeria monocytogenes Plasmids and Expression Levels of Plasmid-Encoded Genes during Growth under Salt and Acid Stress Conditions." Toxins 11, no. 7 (2019): 426. http://dx.doi.org/10.3390/toxins11070426.

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Listeria monocytogenes strains are known to harbour plasmids that confer resistance to sanitizers, heavy metals, and antibiotics; however, very little research has been conducted into how plasmids may influence L. monocytogenes’ ability to tolerate food-related stresses. To investigate this, a library (n = 93) of L. monocytogenes plasmid sequences were compared. Plasmid sequences were divided into two groups (G1 and G2) based on a repA phylogeny. Twenty-six unique plasmid types were observed, with 13 belonging to each of the two repA-based groups. G1 plasmids were significantly (p < 0.05) s

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Cook, L. C., and G. M. Dunny. "Effects of Biofilm Growth on Plasmid Copy Number and Expression of Antibiotic Resistance Genes in Enterococcus faecalis." Antimicrobial Agents and Chemotherapy 57, no. 4 (2013): 1850–56. http://dx.doi.org/10.1128/aac.02010-12.

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ABSTRACTBiofilm growth causes increased average plasmid copy number as well as increased copy number heterogeneity inEnterococcus faecaliscells carrying plasmid pCF10. In this study, we examined whether biofilm growth affected the copy number and expression of antibiotic resistance determinants for several plasmids with diverse replication systems. Four differentE. faecalisplasmids, unrelated to pCF10, demonstrated increased copy number in biofilm cells. In biofilm cells, we also observed increased transcription of antibiotic resistance genes present on these plasmids. The increase in plasmid

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O'Flaherty, Sarah, and Todd R. Klaenhammer. "Multivalent Chromosomal Expression of the Clostridium botulinum Serotype A Neurotoxin Heavy-Chain Antigen and the Bacillus anthracis Protective Antigen in Lactobacillus acidophilus." Applied and Environmental Microbiology 82, no. 20 (2016): 6091–101. http://dx.doi.org/10.1128/aem.01533-16.

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ABSTRACTClostridium botulinumandBacillus anthracisproduce potent toxins that cause severe disease in humans. New and improved vaccines are needed for both of these pathogens. For mucosal vaccine delivery using lactic acid bacteria, chromosomal expression of antigens is preferred over plasmid-based expression systems, as chromosomal expression circumvents plasmid instability and the need for antibiotic pressure. In this study, we constructed three strains ofLactobacillus acidophilusNCFM expressing from the chromosome (i) the nontoxic host receptor-binding domain of the heavy chain ofClostridium

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Kitten, T., and A. G. Barbour. "The relapsing fever agent Borrelia hermsii has multiple copies of its chromosome and linear plasmids." Genetics 132, no. 2 (1992): 311–24. http://dx.doi.org/10.1093/genetics/132.2.311.

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Abstract Borrelia hermsii, a spirochete which causes relapsing fever in humans and other mammals, eludes the immune response by antigenic variation of the "Vmp" proteins. This occurs by replacement of an expressed vmp gene with a copy of a silent vmp gene. Silent and expressed vmp genes are located on separate linear plasmids. To further characterize vmp recombination, copy numbers were determined for two linear plasmids and for the 1-megabase chromosome by comparing hybridization of probes to native DNA with hybridization to recombinant plasmids containing borrelial DNA. Plasmid copy numbers

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Madriz, Xochil, Máximo B. Martínez, Mario A. Rodríguez, et al. "Expression in fibroblasts and in live animals of Entamoeba histolytica polypeptides EhCP112 and EhADH112." Microbiology 150, no. 5 (2004): 1251–60. http://dx.doi.org/10.1099/mic.0.26938-0.

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EhCPADH is an immunogenic, heterodimeric protein that is formed by EhCP112 (cysteine protease) and EhADH112 (adhesin), polypeptides involved in Entamoeba histolytica's cytopathic effect, target-cell adherence and phagocytosis. The EhCPADH complex is located in the plasma membrane and cytoplasmic vacuoles. Here, the independent expression of EhCP112 and EhADH112 in fibroblasts and hamsters was analysed. Also investigated was the immunological response in animals independently inoculated with plasmid pcDNA-Ehcp112, which carries the complete cysteine protease-encoding gene, or with plasmid pcDNA

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Dissertations / Theses on the topic "Expression plasmid"

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Carrington, Francis James. "Plasmid instability and gene expression in E. coli." Thesis, University of Surrey, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293874.

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Eriksson, Jennifer. "Generation of mutated expression plasmid KRT1 and comparison of HaCaT cells transfected with expression plasmid KRT1 or KRT10 concerning keratin aggregates." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-176426.

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Introduction The genetic skin disease epidermolytic ichtyosis is caused by mutations in either keratin gene 1 or 10 and leads to blisters and hyperkeratosis of the epidermis. At cellular level the disease is seen as aggregates in the keratin filaments. Since medicines are hard to investigate and produce mainly due to lack of reproducible model systems, there is no good treatment available for this disease today. In this article we describe how an in vitro model consisting of cells from a stable cell line transfected with expression plasmids to mimic patient cells, may be a possible alternative

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Kühnl, Andrea. "Expression und Reinigung des Plasmid-kodierten Shigellen-Proteins CldpHS₋₂ /." Berlin, 2008. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000253701.

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Salmonowicz, Daniel J. "Creation of a Unique GST-FAK Plasmid for Protein Expression." Ohio Dominican University Honors Theses / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1588678705964411.

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Coons, Terry M. "Restriction mapping and expression of recombinant plasmids containing the arsenic resistance genes of the plasmid R45." PDXScholar, 1986. https://pdxscholar.library.pdx.edu/open_access_etds/3597.

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The trivalent (arsenite) and pentavalent (arsenate) forms of arsenic are introduced into the environment through the use of arsenic in herbicides, pesticides, fertilizers, and the smelting of arsenic-bearing ores. Bacteria resistant to arsenic are readily isolated from surface waters, sewage, and clinical infections. Although some bacterial resistance is provided by inducible phosphate transport systems that discriminate against arsenate, marked resistance is carried on bacterial plasmids. A 6.9 kilobase fragment previously derived from one such plasmid, R45, and containing the genes for induc

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Kwong, Wing Tai Jenny. "Aerosol delivery of cationic lipid/plasmid expression cassettes to lung epithelium." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ29286.pdf.

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Chakravarty, Ashok Hans. "Integration, inheritance and expression of the Agrobacterium rhizogenes Ri plasmid T-DNA." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334788.

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Snaith, Michael. "Applications and expression of proteins encoded by the yeast 2#mu# plasmid." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337410.

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Oliveira, Catarina A. "An investigation into persistent transgene expression from plasmid vectors in the lung." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:033ea326-3e49-4715-9b39-00226792bbba.

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Gene therapy for chronic lung diseases will require vectors capable of persistent transgene expression in the absence of inflammation but few promoters have been identified that can fulfil both of these requirements. The development of plasmids devoid of CG dinucleotides (CpGs) has resulted in markedly reduced levels of pro-inflammatory cytokines. Furthermore, a novel synthetic enhancer/promoter, termed hCEFI, has been shown to direct high levels of sustained transgene expression in the murine lung, following aerosol delivery of non-viral formulations. A CpG-free plasmid containing the hCEFI e

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Pretorius, Inge-Martine. "Studies of the cloning and expression of Thiobacillus Ferrooxidans Plasmid and Nitrogenase genes." Doctoral thesis, University of Cape Town, 1986. http://hdl.handle.net/11427/21904.

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Bibliography : pages 145-156.<br>This dissertation forms part of a fundamental investigation into the molecular biology of the industrially important bacterium, Thiobacillus ferrooxidans. The expression of T. ferrooxidans plasmid encoded functions, as well as the identification, cloning, sequencing and expression in a variety of heterotrophic bacteria and in vitro systems of the T. ferrooxidans nitrogenase structural genes were studied.

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Books on the topic "Expression plasmid"

1

Larson, Jean A. Biotechnology: Ti-plasmid and other plant gene vectors 1985-1988. U.S. Dept. of Agriculture, National Agricultural Library, 1988.

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Larson, Jean A. Biotechnology: Ti-plasmid and other plant gene vectors 1985-1988. U.S. Dept. of Agriculture, National Agricultural Library, 1988.

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Larson, Jean A. Biotechnology: Ti-plasmid and other plant gene vectors 1985-1988. U.S. Dept. of Agriculture, National Agricultural Library, 1988.

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Kwong, Wing Tai Jenny. Aerosol delivery of cationic lipid/plasmid expression cassettes to lung epithelium. National Library of Canada = Bibliothèque nationale du Canada, 1999.

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Theophilus, Bimal David Madhukar. Studies on the expression and regulation of trfA: A gene essential for the replication and maintenance of broad host range plasmid RK2. University of Birmingham, 1987.

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6

Lee, See-Lai. Studies on regulation of expression of the verotoxin operon and of the 39K replication protein of plasmid pFA3 using gene and operon fusions to lac Z. National Library of Canada, 1990.

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7

Chak, Kin-fu. Expression and regulation of ColE plasmids. University of East Anglia, 1985.

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Untersuchungen zur Expression und Funktion des linearen, mitochondrialen Plasmides pC1K1 von Claviceps purpurea. J. Cramer, 1992.

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9

Farnham, Emily. Hofmann: Abstraction as plastic expression and notes made in Hofmann's classes. E. Farnham, 1999.

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Saskia Maria van der Vies. Isolation and expression of a plastid alpha chaperonin cDNA sequence from "triticum aestivum". typescript, 1989.

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Book chapters on the topic "Expression plasmid"

1

Lu, Quinn. "Plasmid Vectors for Gene Cloning and Expression." In Plasmid Biology. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817732.ch27.

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Lara, Alvaro R., and Octavio T. Ramírez. "Plasmid DNA Production for Therapeutic Applications." In Recombinant Gene Expression. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-433-9_14.

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Lara, Alvaro R., and Octavio T. Ramírez. "Plasmid DNA Production for Therapeutic Applications." In Recombinant Gene Expression. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-433-9_35.

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Fioretti, Daniela, Sandra Iurescia, and Monica Rinaldi. "Enhancement of Plasmid-Mediated Transgene Expression." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0410-5_2.

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Gunsalus, I. C. "Notes on Metabolic Plasmid Organization and Expression." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_48.

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Manthorpe, Marston, Jukka Hartikka, H. Lee Vahlsing, and Michael Sawdey. "Quantification of Plasmid DNA Expression in Vivo." In Gene Quantification. Birkhäuser Boston, 1998. http://dx.doi.org/10.1007/978-1-4612-4164-5_20.

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Nordstrom, J. L. "Plasmid-Based Gene Transfer and Antiprogestin-Controllable Transgene Expression." In Human Gene Therapy: Current Opportunities and Future Trends. Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-662-05352-2_14.

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Ponder, Katherine Parker. "Gene Delivery for Systemic Expression: Plasmid, Retroviral, and Adenoviral Approaches." In Gene Transfer in the Cardiovascular System. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-6277-1_18.

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Lale, Rahmi, Trygve Brautaset, and Svein Valla. "Broad-Host-Range Plasmid Vectors for Gene Expression in Bacteria." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-197-0_19.

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Peretti, Steven W., and James D. Bryers. "Effects of Biofilm Formation on Plasmid Segregational Stability and Expression." In Biofilms — Science and Technology. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1824-8_18.

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Conference papers on the topic "Expression plasmid"

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Paproski, Robert J., and Roger J. Zemp. "Inducible expression of photoacoustic reporter gene tyrosinase in cells using a single plasmid." In SPIE BiOS, edited by Alexander A. Oraevsky and Lihong V. Wang. SPIE, 2012. http://dx.doi.org/10.1117/12.909644.

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Bjorklid, E., T. Johansen, U. R. Pendurthi, L. V. M. Rao, B. Warn-Cramer, and S. T. Rapaport. "HUMAN cDNA CLONES FOR THROMBOPLASTIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643735.

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A rabbit polyclonal antibody which was monospecific for thromboplastin (TP) apoprotein from human brain according to a number of criteria was used to screen two human placenta cDNA libraries in the expression vector λgt11, one randomly primed and one oligo-dT primed, by the method of Young and Davis. 23 positive clones expressing TP related antigen were isolated and plaque purified. DNA from the different clones was isolated and the TPcDNA inserts released by EcoRl digestion. The inserts could be classified into11 size classes ranging from approx. 300-1100 base pairs.The largest insert (1100 b

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Wha Lin, Shu, J. Ware, H. Roberts, N. McGraw, W. McAllister, and D. Stafford. "EXPRESSION OF HUMAN FACTOR IX IN MAMMALIAN CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643567.

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Human factor IX has been expressed in mammalian cells. A cloned factor IX cDNA missing the first 15 nucleotides of the 5’ end was modified by in vitro mutagenesis to restore the missing codons and add the translation consensus sequence, CCACC, proposed by Kozak to be optimal for translational initiation. Additionally, Bgl II and BamHI sites were added immediately upstream of the CCACC sequence for ease of portability of the fragment. This modified cDNA was inserted into a bovine papillomavirus (BPV) vector under the control of a mouse met alio thionein promoter. The constructed plasmid pBPV-IX

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Klueva, Svetlana N., Vladimir N. Korsukov, Tatyana N. Schukovskaya, and Alexander L. Kravtsov. "Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content." In SPIE Proceedings, edited by Valery V. Tuchin. SPIE, 2004. http://dx.doi.org/10.1117/12.579011.

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Himmah, Karimatul, Nurul Dluha, Nadya V. M. Anyndita, Muhaimin Rifa’i, and Widodo. "Design and evaluation of protein expression in a recombinant plasmid encoding epitope gp 350/220 of the Epstein-Barr virus (EBV)." In 2ND INTERNATIONAL CONFERENCE ON COMPOSITE MATERIALS AND MATERIAL ENGINEERING (ICCMME 2017). Author(s), 2017. http://dx.doi.org/10.1063/1.4983432.

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Sirr, Noel, Doina Ciobanu, Ronan Grimes, and Mark Davies. "A Continuous Flow Polymerase Chain Reactor for DNA Expression Analysis." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96180.

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The polymerase chain reaction (PCR) has revolutionised molecular biology, and is at the forefront of many current efforts to document and understand human genetic diversity. Recent years has seen a move towards incorporating the PCR technique into a micro Total Analysis System (μTAS) thus exploiting its full potential. Micro scale PCR design offers the opportunity to integrate all functional steps of DNA expression analysis into a miniaturised device allowing for high throughput and reduced analysis times, reduced sample volume requirements and cost efficiency. Consequently, it is desirable to

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Kang, Young Bok (Abraham), Joseph Cirillo, Siddhartha Rawat, Michael Bouchard, and Hongseok (Moses) Noh. "Layered Hepatocytes and Endothelial Cells on a Transwell Membrane: Toward Engineering the Liver Sinusoid." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-89413.

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This paper presents a novel liver model platform that mimics the liver sinusoid, a functional unit of the liver where most liver activities occur. A key component of the current liver model is a layered co-culture of primary rat hepatocytes (PRH) and primary rat liver sinusoidal endothelial cells (LSEC) or a bovine aortic endothelial cells (BAEC) as an alternative. Poly-dimethylsiloxane (PDMS) microchannels were fabricated and attached to transwell membranes that contain submicroscale pores. Cells were cultured either on one side or on both sides of the transwell membrane, and in both cases ce

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Homes, W. E., H. R. Lijnen, L. Nelles, C. Kluft та D. Collen. "AN ALANINE INSERTION IN α2-ANTIPLASMIN ‘ENSCHEDE’ ABOLISHES ITS PLASM IN INHIBITORY ACTIVITY". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642897.

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Congenital deficiencies of the fibrinolytic inhibitor α2antiplasmin (α2AP) may result in bleeding disorders. An abnormal a AP (α2AP‘Enschede’) is known. 2 siblings with 3% functional activity and normal antigen level have parents with 50% activity and normal antigen. The protein interacts normally with the lysine-binding site(s) of plasmin(ogen) but does not inhibit plasmin irreversibly. α2AP Enschede is a plasmin substrate that like the normal protein releases a M 8,000 peptide upon reaction with plasmin. In the present study, Southern blot analysis, using an α2AP cDNA probe showed a restrict

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Olton, Dana, Dong Hyun Lee, Charles Sfeir, and Prashant N. Kumta. "Novel Nanostructured Calcium Phosphate Based Delivery Systems for Non-Viral Gene Delivery." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176286.

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Calcium phosphate (CaP) based approaches remain an attractive option for delivering plasmid DNA (pDNA) into cultured cells [1, 2]. However, two major limitations associated with this vector exist. First, it yields lower transfection efficiencies with respect to its’ viral counterparts. Second, CaP mediated gene delivery leads to transient transgene expression. Thus, we hypothesized that these concerns could respectively be addressed by: (1) synthesizing particles with precise control of the materials’ parameters including (i.e. Ca/P ratio, particle size, crystal structure, and microstructure)

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Lord, S. T. "DIRECTED MUTAGENESIS OF HUMAN FIBRINOGEN: Aα CHAIN SUBSTITUTIONS THAT ALTER THROMBIN CLEAVAGE AND ANTIBODY RECOGNITION". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642887.

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The initial event in fibrin clot formation is the thrombin catalized cleavage of the Aa chain of fibrinogen between Argl6 and Glyl7, releasing fibrinopeptide A. Previous data indicate that most of the information required for thrombin recognition and cleavage of the Aa chain lies within the amino terminal 51 residue CNBr fragment. In order to use protein engineering techniques to study the interaction of thrombin with the Aa chain, we have constructed a plasmid expression vector which encodes a tripartite protein consisting of amino acids 1-50 of the Aa chain of human fibrinogen followed by 60

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Reports on the topic "Expression plasmid"

1

Coons, Terry. Restriction mapping and expression of recombinant plasmids containing the arsenic resistance genes of the plasmid R45. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.5481.

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Arnett, Clint, Justin Lange, Ashley Boyd, Martin Page, and Donald Cropek. Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41546.

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Cationic polypeptide proteins found in the seeds of the tropical plant Moringa oleifera have coagulation efficiencies similar to aluminum and ferric sulfates without their recalcitrant nature. Although these proteins possess great potential to augment or replace traditional coagulants in water treatment, harvesting active protein from seeds is laborious and not cost-effective. Here, we describe an alternative method to express and secrete active M. oleifera coagulant protein (MO) in Bacillus subtilis. A plasmid library containing the MO gene and 173 different types of secretory signal peptides

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Underwood, J. H., J. J. Keating, E. Troiano, and A. P. Parker. Expression for Calculating Plastic Radius, c, from Slit Opening of a Disk from an Autofrettaged Tube. Defense Technical Information Center, 2010. http://dx.doi.org/10.21236/ada589992.

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VanHouten, Joshua N. Does Increased Expression of the Plasma Membrane Calcium-ATPase Isoform 2 Confer Resistance to Apoptosis on Breast Cancer Cells? Defense Technical Information Center, 2008. http://dx.doi.org/10.21236/ada493700.

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