Academic literature on the topic 'Extended-spectrum β-lactamase-producing strains of Enterobacteriacae'
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Journal articles on the topic "Extended-spectrum β-lactamase-producing strains of Enterobacteriacae"
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Amare, Azanaw, Setegn Eshetie, Desie Kasew, and Feleke Moges. "High prevalence of fecal carriage of Extended-spectrum beta-lactamase and carbapenemase-producing Enterobacteriaceae among food handlers at the University of Gondar, Northwest Ethiopia." PLOS ONE 17, no. 3 (2022): e0264818. http://dx.doi.org/10.1371/journal.pone.0264818.
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Background Fecal carriage of extended-spectrum beta-lactamase and Carbapenemase-producing Enterobacteriaceae is a potential risk for the transmission of infection with resistant strains. Understanding the burden of these resistant strains in asymptomatic people is essential to reduce the chain of infection transmission. However, data on the fecal carriage of Extended-spectrum Beta-lactamase and Carbapenemase-producing Enterobacteriaceae among food handlers were limited in developing countries especially in Ethiopia. The aim of the present study is, therefore, to assess fecal carriage rate, associated factors, and antimicrobial resistance patterns of Extended-spectrum Beta-lactamase and Carbapenemase-producing Enterobacteriaceae among food handlers at the University of Gondar Cafeterias, Northwest Ethiopia. Materials and methods An institution-based cross-sectional study was conducted from February to June 2021 at the University of Gondar cafeterias. A total of 290 stool samples were collected, transported using Cary Blair transport medium, and processed. All isolates were cultured and identified by using MacConkey agar, and routine biochemical tests. Antimicrobial susceptibility testing was done to each isolate following the Kirby Bauer disk diffusion method. If the zone of inhibition was ≤ 22 mm for ceftazidime, ≤25 mm for ceftriaxone, and ≤27 for cefotaxime they were considered as potential ESBL strain and selected for a further phenotypic confirmatory. Moreover, the double-disc diffusion test and the modified carbapenem inactivation method were used for confirmations of Extended-spectrum β-lactamase and Carbapenemase-producing Enterobacteriaceae respectively. If a ≥5mm difference in zone diameter for either antimicrobial agent in combination with clavulanic acid versus the zone diameter of the agent when tested alone (without B-lactamase inhibitor), was confirmed as ESBL-PE and if the zone of inhibition diameter between 6-15mm and 16- 18mm with a pinpoint colony, it was considered as carbapenem resistance Enterobacteriaceae. Data were entered using Epi-data version 4.6 and then exported to SPSS version 26 for analysis. Potential risk factors were assessed using multivariable logistic regression and a p-value less than 0.05 was considered statistically significant. Results Out of 290 stool samples, 63 (21.7%) and 7 (2.4%) were confirmed as Extended-spectrum β-lactamase and Carbapenemase-producing Enterobacteriaceae. The most predominant ESBL-PE was E. coli 43 (14.8%) followed by K. pneumoniae 17 (5.9%). Most of the Extended-spectrum β-lactamase and Carbapenemase-producing isolates were resistant to tetracycline, cefotaxime, ceftazidime, and ceftriaxone (100% each). In contrast, a low resistance level was recorded for Meropenem and cefoxitin. The overall Multi-drug resistant Enterobacteriaceae (MDR) was 147 (42.3%). Antibiotics usage in the last 3 months and drinking unpasteurized milk were associated with the carriage of the Extended-spectrum beta-lactamase-Producing Enterobacteriaceae. Conclusions and recommendations The high fecal carriage rate of Multi-drug resistance isolate, Extended-spectrum β-lactamase, and Carbapenemase-producing Enterobacteriaceae were recorded among food handlers. Therefore, this study gives signals in the spread of drug-resistant bacteria easily to the community. Hence, the need for adjusting and promotion of infection prevention measures to prevent the spread of drug-resistant bacteria should not be underestimated.
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Cantón, Rafael, María Pérez-Vázquez, Antonio Oliver, et al. "Evaluation of the Wider System, a New Computer-Assisted Image-Processing Device for Bacterial Identification and Susceptibility Testing." Journal of Clinical Microbiology 38, no. 4 (2000): 1339–46. http://dx.doi.org/10.1128/jcm.38.4.1339-1346.2000.
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The Wider system is a newly developed computer-assisted image-processing device for both bacterial identification and antimicrobial susceptibility testing. It has been adapted to be able to read and interpret commercial MicroScan panels. Two hundred forty-four fresh consecutive clinical isolates (138 isolates of the familyEnterobacteriaceae, 25 nonfermentative gram-negative rods [NFGNRs], and 81 gram-positive cocci) were tested. In addition, 100 enterobacterial strains with known β-lactam resistance mechanisms (22 strains with chromosomal AmpC β-lactamase, 8 strains with chromosomal class A β-lactamase, 21 broad-spectrum and IRT β-lactamase-producing strains, 41 extended-spectrum β-lactamase-producing strains, and 8 permeability mutants) were tested. API galleries and National Committee for Clinical Laboratory Standards (NCCLS) microdilution methods were used as reference methods. The Wider system correctly identified 97.5% of the clinical isolates at the species level. Overall essential agreement (±1 log2dilution for 3,719 organism-antimicrobial drug combinations) was 95.6% (isolates of the family Enterobacteriaceae, 96.6%; NFGNRs, 88.0%; gram-positive cocci, 95.6%). The lowest essential agreement was observed with Enterobacteriaceae versus imipenem (84.0%), NFGNR versus piperacillin (88.0%) and cefepime (88.0%), and gram-positive isolates versus penicillin (80.4%). The category error rate (NCCLS criteria) was 4.2% (2.0% very major errors, 0.6% major errors, and 1.5% minor errors). Essential agreement and interpretive error rates for eight β-lactam antibiotics against isolates of the family Enterobacteriaceae with known β-lactam resistance mechanisms were 94.8 and 5.4%, respectively. Interestingly, the very major error rate was only 0.8%. Minor errors (3.6%) were mainly observed with amoxicillin-clavulanate and cefepime against extended-spectrum β-lactamase-producing isolates. The Wider system is a new reliable tool which applies the image-processing technology to the reading of commercial trays for both bacterial identification and susceptibility testing.
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Stewardson, Andrew J., Gesuele Renzi, Nathalie Maury та ін. "Extended-Spectrum β-Lactamase–Producing Enterobacteriaceae in Hospital Food: A Risk Assessment". Infection Control & Hospital Epidemiology 35, № 4 (2014): 375–83. http://dx.doi.org/10.1086/675609.
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Objective.Determine the prevalence of extended-spectrum β-lactamase (ESBL)–producing Enterobacteriaceae (ESBL-PE) contamination of food and colonization of food handlers in a hospital kitchen and compare retrieved ESBL-PE strains with patient isolates.Design.Cross-sectional study.Setting.A 2,200-bed tertiary care university hospital in Switzerland.Participants.Food handlers.Methods.Raw and prepared food samples were obtained from the hospital kitchen, with a comparator group from local supermarkets. Fecal samples collected from food handlers and selectively pre-enriched homogenized food samples were inoculated onto selective chromogenic media. Phenotypic confirmation of ESBL production was performed using the double disk method. Representative ESBL-PE were characterized using polymerase chain reaction (PCR) and sequencing for blaCTX-M, blaSHV, and blaTEM genes, and Escherichia coli strains were typed using phylotyping, repetitive element palindromic PCR, and multilocus sequence typing. Meat samples were screened for antibiotic residues using liquid chromatography time-of-flight mass spectrometry.Results.Sixty (92%) of the raw chicken samples were ESBL-PE positive, including 30 (86%) of the hospital samples and all supermarket samples. No egg, beef, rabbit, or cooked chicken samples were ESBL-PE positive. No antibiotic residues were detected. Six (6.5%) of 93 food handlers were ESBL-PE carriers. ESBL-PE strains from chicken meat more commonly possessed blaCTX-M-1 and blaCTX-M-2, whereas blaCTX-M-14and blaCTX-M-15 were predominant among strains of human origin. There was partial overlap in the sequence type of E. coli strains of chicken and human origin. No E. coli ST131 strains or blaCTX-M-15 genes were isolated from meat.Conclusions.Although there is significant ESBL-PE contamination of delivered chicken meat, current preventive strategies minimize risks to food handlers, hospital staff, and patients.
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Gekenidis, Maria-Theresia, Anita Kläui, Kornelia Smalla та David Drissner. "Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in Enterobacteriaceae from Irrigation Water". Microorganisms 8, № 7 (2020): 978. http://dx.doi.org/10.3390/microorganisms8070978.
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Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are classified as serious threats to human health by the U.S. Centers for Disease Control and Prevention. Water used for irrigation of fresh produce can transmit such resistant bacteria directly to edible plant parts. We screened ESBL-producing Escherichia coli, Enterobacter cloacae, and Citrobacter freundii isolated from irrigation water for their potential to transmit resistance to antibiotic-susceptible E. coli. All strains were genome-sequenced and tested in vitro for transmission of resistance to third-generation cephalosporins on solid agar as well as in liquid culture. Of the 19 screened isolates, five ESBL-producing E. coli were able to transfer resistance with different efficiency to susceptible recipient E. coli. Transconjugant strains were sequenced for detection of transferred antibiotic resistance genes (ARGs) and compared to the known ARG pattern of their respective donors. Additionally, phenotypic resistance patterns were obtained for both transconjugant and corresponding donor strains, confirming ESBL-producing phenotypes of all obtained transconjugants.
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Rahma, Trabelsi, Yengui Mariem, Mhaya Amel, Rebai Ahmed, Arpin Corinne, and Gdoura Radhouane. "Detection of extended-spectrum beta-lactamase and scarbapenemase-producing Enterobacteriaceae in Tunisia." Archives of Biotechnology and Biomedicine 7, no. 1 (2023): 001–11. http://dx.doi.org/10.29328/journal.abb.1001034.
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The emergence of dramatic urinary tract infections (UTIs) caused by the members of the Enterobacteriales is an important public health problem in the community as well as in Tunisian hospitals. This study aims to investigate the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing uropathogenic isolates of Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae). Based on decreased susceptibility to β-lactams antibiotics and analyzed for the presence of ESBL and carbapenemase genes by Real Time- polymerase chain reaction (RT-PCR), 56 uropathogenic isolates of E. coli (n = 36) and K. pneumoniae (n = 20) were confirmed positive for ESBLs. The CTX-M-type β-lactamases were mostly detected in E. coli isolates (21 strains, 58.33% [95% CI 38.09% - 72.06%]) followed by blaSHV-like (18 strains, 50% [95% CI 32.92% - 67.07%]), blaTEM-like and blaCMY-2-like simultaneously (15 strains, 41.67% [95% CI 25.51% - 59.24%]). Furthermore, the RT-PCR system on the K. pneumoniae strains demonstrated that blaSHV-12-like was the most predominant (16 strains, 80% [95% CI 56.33% - 94.26%]) followed by blaTEM-like (14 strains, 70% [95% CI 45.72% - 88.10%]), blaCTX-M belonging to groups 9 and 1 (11 strains, 55% [95% CI 31.52% - 76.94%]) and finally blaCMY-2-like (10 strains, 50% [95% CI 27.19% - 72.80%]). In addition, E. coli and K. pneumoniae strains harbored a carbapenemase gene blaOXA-48-like with 22.2% [95% CI 10.11% - 39.15%]; 20% [95% CI 12.83% - 43.66%], respectively. Our results confirm the need to monitor the resistance to extended-spectrum β-lactams and to carbapenems among enterobacteria in Tunisia.
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GESER, N., R. STEPHAN, P. KUHNERT та ін. "Fecal Carriage of Extended-Spectrum β-Lactamase–Producing Enterobacteriaceae in Swine and Cattle at Slaughter in Switzerland". Journal of Food Protection 74, № 3 (2011): 446–49. http://dx.doi.org/10.4315/0362-028x.jfp-10-372.
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During the past decade, extended-spectrum β-lactamase (ESBL)–producing Enterobacteriaceae have become a matter of great concern in human medicine. ESBL-producing strains are found in the community, not just in hospital-associated patients, which raises a question about possible reservoirs. Recent studies describe the occurrence of ESBL-producing Enterobacteriaceae in meat, fish, and raw milk; therefore, the impact of food animals as reservoirs for and disseminators of such strains into the food production chain must be assessed. In this pilot study, fecal samples of 59 pigs and 64 cattle were investigated to determine the occurrence of ESBL-producing Enterobacteriaceae in farm animals at slaughter in Switzerland. Presumptive-positive colonies on Brilliance ESBL agar were subjected to identification and antibiotic susceptibility testing including the disc diffusion method and E-test ESBL strips. As many as 15.2% of the porcine and 17.1% of the bovine samples, predominantly from calves, yielded ESBL producers. Of the 21 isolated strains, 20 were Escherichia coli, and one was Citrobacter youngae. PCR analysis revealed that 18 strains including C. youngae produced CTX-M group 1 ESBLs, and three strains carried genes encoding for CTX-M group 9 enzymes. In addition, eight isolates were PCR positive for TEM β-lactamase, but no blaSHV genes were detected. Pulsed-field gel electrophoresis showed a high genetic diversity within the strains. The relatively high rates of occurrence of ESBL-producing strains in food animals and the high genetic diversity among these strains indicate that there is an established reservoir of these organisms in farm animals. Further studies are necessary to assess future trends.
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Wang, Hui, Swathi Kelkar, Weiyuan Wu, Minjun Chen та John P. Quinn. "Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China". Antimicrobial Agents and Chemotherapy 47, № 2 (2003): 790–93. http://dx.doi.org/10.1128/aac.47.2.790-793.2003.
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ABSTRACT The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.
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Arpin, Corinne, Véronique Dubois, Laure Coulange та ін. "Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Community and Private Health Care Centers". Antimicrobial Agents and Chemotherapy 47, № 11 (2003): 3506–14. http://dx.doi.org/10.1128/aac.47.11.3506-3514.2003.
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ABSTRACT In 1999, 39 of 2,599 isolates of the family Enterobacteriaceae (1.5%) collected by eight private laboratories in the Aquitaine region in France produced an extended-spectrum β-lactamase (ESBL). Among these were 19 Enterobacter aerogenes isolates; 8 Klebsiella pneumoniae isolates; 6 Escherichia coli isolates; 3 Proteus mirabilis isolates; and 1 isolate each of Serratia marcescens, Morganella morganii, and Providencia stuartii. ESBL producers were isolated from 38 patients, including 33 residents of 11 clinics or nursing homes and 5 ambulatory patients. Seven different ESBLs were characterized. These mainly consisted of TEM-24 (25 isolates) and TEM-21 (9 isolates), but TEM-15 (2 isolates) and TEM-3, TEM-19, SHV-4, and CTX-M-1 (1 isolate each) were also characterized. Seven strains showed the coexistence of different TEM- and/or SHV-encoding genes, including a new SHV-1 variant, SHV-44, defined by the substitution R205L previously reported for SHV-3 in association with S238G. The epidemiology of the ESBL producers was investigated by random amplification of polymorphic DNA, typing by enterobacterial repetitive intergenic consensus PCR, analysis of resistance cotransferred with the ESBL, and analysis of the restriction profiles of the ESBL-encoding plasmids. Of the TEM-24-expressing strains, 18 were E. aerogenes isolates, including 9 from the same clinic, that were representatives of the epidemic clone disseminating in France. Of the TEM-21-producing strains that belonged to different species of the family Enterobacteriaceae (E. coli, K. pneumoniae, and P. mirabilis), 8 were isolated in the same nursing home. Outbreaks due to strain and/or plasmid dissemination in these clinic and nursing home were demonstrated. The presence of ESBL producers in five ambulatory patients probably resulted from nosocomial acquisition. Our data highlight the serious need to monitor patients for ESBL-producing Enterobacteriaceae in general practice.
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Spanu, T., F. Luzzaro, M. Perilli та ін. "Occurrence of Extended-Spectrum β-Lactamases in Members of the Family Enterobacteriaceae in Italy: Implications for Resistance to β-Lactams and Other Antimicrobial Drugs". Antimicrobial Agents and Chemotherapy 46, № 1 (2002): 196–202. http://dx.doi.org/10.1128/aac.46.1.196-202.2002.
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ABSTRACT An Italian nationwide survey was carried out to assess the prevalences and the antimicrobial susceptibilities of members of the family Enterobacteriaceae producing extended-spectrum β-lactamases (ESBLs). Over a 6-month period, 8,015 isolates were obtained from hospitalized patients and screened for resistance to extended-spectrum cephalosporins and monobactams. On the basis of a synergistic effect between clavulanate and selected β-lactams (ceftazidime, aztreonam, cefotaxime, cefepime, and ceftriaxone), 509 isolates were found to be ESBL positive (6.3%). Colony blot hybridization with bla TEM and bla SHV DNA probes allowed one to distinguish four different genotypes: TEM-positive, SHV-positive, TEM- and SHV-positive, and non-TEM, non-SHV ESBL types. MICs for each isolate (E-test) were obtained for widely used β-lactams, combinations of β-lactams with β-lactamase inhibitors, aminoglycosides, and fluoroquinolones. Among ESBL-positive strains, Klebsiella pneumoniae, Proteus mirabilis, and Escherichia coli accounted for 73.6% of isolates. Overall, TEM-type ESBLs were more prevalent than SHV-type enzymes (234 versus 173), whereas the prevalence of strains producing both TEM- and SHV-type ESBLs was similar to that of isolates producing non-TEM, non-SHV enzymes (55 and 38, respectively). In vitro, all but one of the ESBL-producing isolates remained susceptible to imipenem. Susceptibility to other drugs varied: piperacillin-tazobactam, 91%; amoxicillin-clavulanic acid, 85%; cefoxitin, 78%; amikacin, 76%; ampicillin-sulbactam, 61%; ciprofloxacin, 58%; and gentamicin, 56%. Associated resistance to aminoglycosides and ciprofloxacin was observed most frequently among TEM-positive strains. Since therapeutic options for multiresistant Enterobacteriaceae are limited, combinations of β-lactams and β-lactamase inhibitors appear to represent an important alternative for treating infections caused by ESBL-producing Enterobacteriaceae.
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Arpin, Corinne, Laure Coulange, Véronique Dubois та ін. "Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae Strains in Various Types of Private Health Care Centers". Antimicrobial Agents and Chemotherapy 51, № 9 (2007): 3440–44. http://dx.doi.org/10.1128/aac.01431-06.
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ABSTRACT During a 2004 survey, 49 extended-spectrum-β-lactamase-producing enterobacteria were collected in 20 French private health care centers and one local hospital. They included 12 CTX-M-producing Escherichia coli strains (1.8% versus 0.3% in a 1999 survey). Most of them belonged to the same clone and contained a bla CTX-M-15 gene on similar conjugative plasmids.
Dissertations / Theses on the topic "Extended-spectrum β-lactamase-producing strains of Enterobacteriacae"
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Ferreira, Helena Cardoso de Carvalho. "Antibioresistance of Extended-Spectrum β-Lactamase producer Enterobacteriaceae strains from broiler samples". Bachelor's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2008. http://hdl.handle.net/10400.5/878.
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Dissertação de Mestrado Integrado em Medicina Veterinária<br>O objectivo deste estudo foi determinar em que medida a higiene do matadouro influenciava a produção de β-lactamases de espectro alargado (ESBL’s) em Salmonella e outras Enterobacteriaceae. Para tal, foram colhidas amostras em vários pontos da linha de abate de frangos de engorda (“broiler”) num matadouro na Holanda. A partir dos dados recolhidos quantificou-se o número de Enterobacteriaceae e isolou-se Salmonella spp.. Testou-se a resistência aos seguintes antibióticos: ampicillina, ceftiofur, trimethoprim+sulfametoxazol, eritromicina, tetraciclina, gentamicina e enrofloxacina em todos os isolados. Assumiu-se que os isolados resistentes a ceftiofur, eram produtores de β-lactamases de espectro alargado. Do total de isolados recolhidos (n=389) verificou-se que 57.5% eram produtores de ESBL e que 57.1% apresentavam resistência a ceftiofur e outros antibióticos. Já os isolados de Salmonella revelaram uma percentagem de bactérias produtoras de ESBL de 77.3% e de bactérias multiresistentes de 77.3%. A resistência combinada de ceftiofur e enrofloxacina era de 15.7% no total de isolados e de 13.6% nos isolados de Salmonella. O elevado número de isolados resistentes mostrou que deve ser exercido controlo durante toda a cadeia de produção para que seja possível prevenir a transmissão de resistência entre diferentes animais e o Homem. Este estudo confirma a necessidade de avaliar o papel dos isolados bacterianos provenientes de animais como reservatórios de resistência a antibióticos.<br>ABSTRACT
The aim of this study was to evaluate the effect of slaughterhouse contamination on the presence of antimicrobial resistant bacterial isolates, specifically Salmonella and other members of the Enterobacteriaceae family. Samples were collected in different points in a poultry slaughterhouse in The Netherlands, and after quantification of Enterobacteriaceae and isolation of Salmonella spp., antibiograms were made of the identified isolates.The following antibiotics were tested: ampicillin, ceftiofur, trimethoprim+sulfamethoxazole, erythromycin, tetracycline, gentamicin and enrofloxacin. The isolates that were resistant to ceftiofur were considered ESBL positive.
Fom the total isolated strains (n=389), 57.5% were ESBL positive and 57.1% were resistant to ceftiofur and other antibiotics. Salmonella showed a prevalence of 77.3% ESBL positive isolates and 77.3% presenting a multiresistance profile. The combination of ceftiofur and enrofloxacin resistance in the Salmonella isolates was present in 13.6% and 15.7% on the total collected strains. The high number of resistant strains found shows that control should be assured throughout the production chain in order to prevent transmission of resistant determinants between different animals and humans as much as possible. This study also confirms the need to further evaluate the role of food animal bacterial isolates as reservoirs for antibiotic resistance.
Book chapters on the topic "Extended-spectrum β-lactamase-producing strains of Enterobacteriacae"
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Bonsu Karikari, Akosua, Courage Kosi Setsoafia Saba, and David Yembilla Yamik. "Bacterial Resistance in Urinary Tract Infections: Multidrug Resistant ESBL Producing Gram Negative Uropathogens from Patients." In Urinary Tract Infection and Nephropathy - Insights into Potential Relationship [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98466.
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Urinary tract infection is one of the most common bacterial infectious diseases encountered in clinical practice. The development and spread of multidrug resistant isolates are of great global health burden; among them, extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae has been a prime concern. This topic describes the resistance patterns of eighty three (83) Gram negative uropathogens to different classes of antibiotics. Bacteria isolates were obtained from patients of all age groups who sought medical attention at a secondary and tertiary hospital in Northern Ghana. Culture and isolation methods employed were the quantitative urine culture on Cysteine Lysine Electrolyte Deficient (CLED) agar and standard biochemical tests. ESBL production was detected using the CLSI recommended phenotypic confirmatory test along with routine antibiotic susceptibility test, adopting the Kirby-Bauer disk diffusion method. Out of 83 isolates, seven (7) Gram negative uropathogens were characterized and ESBLs were detected in 32 of the isolates. Escherichia coli was the pathogen with most ESBL positive strains. Generally high and multiple drug resistance were recorded in both ESBL and non-ESBL strains to the empirical drugs, however, ESBL positive strains significantly (p = 0.000) showed greater resistance. A notable finding was the appreciable resistance exhibited by ESBL strains to last line treatment drugs that include aminoglycosides and imipenem.
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Dembélé, René, Wendpoulomdé A.D. Kaboré, Issiaka Soulama, et al. "Beta-Lactamase-Producing Genes and Integrons in Escherichia coli from Diarrheal Children in Ouagadougou, Burkina Faso." In Diarrhea - Novel Advances and Future Perspectives in the Etiological Diagnosis and Management [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.103169.
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This study aimed to determine the resistance of diarrheagenic Escherichia coli (DEC) strains to β-lactams antibiotics and to perform the molecular characterization of extended-spectrum β-lactamases (ESBLs) and integrons genes. It was carried out from August 2013 to October 2015 and involved 31 DEC strains isolated from diarrheal stools samples collected from children less than 5 years. The identification and characterization of DEC strains were done through the standard biochemical tests that were confirmed using API 20E and polymerase chain reaction (PCR). The antibiogram was realized by the disk diffusion method, then an amplification of the β-lactamase resistance genes and integrons by PCR was done. Out of the 419 E. coli, 31 isolates (7.4%) harbored the DEC virulence genes. From these DEC, 21 (67.7%) were ESBL-producing E. coli. Susceptibility to ESBL-producing E. coli showed that the majority of isolates were highly resistant to amoxicillin (77.4%), amoxicillin-clavulanic acid (77.4%), and piperacillin (64.5%). The following antibiotic resistance genes and integron were identified: blaTEM (6.5%), blaSHV (19.4%), blaOXA (38.7%), blaCTX-M (9.7%), Int1 (58.1%), and Int3 (19.4%). No class 2 integron (Int2) was characterized. Because of the high prevalence of multidrug-resistant ESBL organisms found, there is a need of stringent pediatric infection control measures.