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1

Li, Ningjie, Linbo Fu, Lei Wu, Zhongwei Chen, and Qi Lan. "Influence of culture conditions on extracellular polymeric substances production by the white rot fungi Phanerochaete chrysosporium." MATEC Web of Conferences 175 (2018): 01004. http://dx.doi.org/10.1051/matecconf/201817501004.

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The extracellular polymeric substances of white rot fungi play an important role in the adsorption of heavy metals, but the influence of culture conditions on extracellular polymeric substances production is still unknown. In this paper, we researched on the influence of temperature, incubation time, the rotational speed and the inoculation volume on the yield of extracellular polymeric substances produced by Phanerochaete chrysosporium, a model strain of white rot fungi. The results show that the optimum culture conditions for Phanerochaete chrysosporium to produce extracellular polymeric sub
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2

Bello-Morales, Raquel, Sabina Andreu, Vicente Ruiz-Carpio, Inés Ripa, and José Antonio López-Guerrero. "Extracellular Polymeric Substances: Still Promising Antivirals." Viruses 14, no. 6 (2022): 1337. http://dx.doi.org/10.3390/v14061337.

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Sulfated polysaccharides and other polyanions have been promising candidates in antiviral research for decades. These substances gained attention as antivirals when they demonstrated a high inhibitory effect in vitro against human immunodeficiency virus (HIV) and other enveloped viruses. However, that initial interest was followed by wide skepticism when in vivo assays refuted the initial results. In this paper we review the use of sulfated polysaccharides, and other polyanions, in antiviral therapy, focusing on extracellular polymeric substances (EPSs). We maintain that, in spite of those ear
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3

Zhang, Xiaoqi, and Paul L. Bishop. "Biodegradability of biofilm extracellular polymeric substances." Chemosphere 50, no. 1 (2003): 63–69. http://dx.doi.org/10.1016/s0045-6535(02)00319-3.

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4

Chen, Ming‐Yuan, Duu‐Jong Lee, and J. H. Tay. "Extracellular Polymeric Substances in Fouling Layer." Separation Science and Technology 41, no. 7 (2006): 1467–74. http://dx.doi.org/10.1080/01496390600683597.

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5

Gong, Amy S., Carl H. Bolster, Magda Benavides, and Sharon L. Walker. "Extraction and Analysis of Extracellular Polymeric Substances: Comparison of Methods and Extracellular Polymeric Substance Levels inSalmonella pullorumSA 1685." Environmental Engineering Science 26, no. 10 (2009): 1523–32. http://dx.doi.org/10.1089/ees.2008.0398.

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6

Li, Qiang, Ge Hu, Peng Song, et al. "Membrane fouling of actual extracellular polymeric substances." IOP Conference Series: Earth and Environmental Science 647 (January 27, 2021): 012112. http://dx.doi.org/10.1088/1755-1315/647/1/012112.

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7

Ahsan, Nazmul, Kashfia Faruque, Farah Shamma, Nazrul Islam, and Anwarul A. Akhand. "Arsenic adsorption by Bacterial Extracellular Polymeric Substances." Bangladesh Journal of Microbiology 28, no. 2 (2012): 80–83. http://dx.doi.org/10.3329/bjm.v28i2.11821.

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The main objective of this work was to isolate arsenic resistant bacteria from contaminated soil, followed by screening for their ability to adsorb arsenic. Six bacterial isolates (S1 to S6) were obtained from arsenic contaminated soil samples and among these, five (S1, S2, S3, S5 and S6) were characterized as bacillus and the rest one (S4) was cocci depending on shape. All the isolates except S6 produced extracellular polymeric substances (EPS) in the culture medium and displayed arsenic adsorbing activities demonstrated by adsorption of around 90% from initial concentration of 1 mg/L sodium
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8

Zhang, Guojun, Shulan Ji, Xue Gao, and Zhongzhou Liu. "Adsorptive fouling of extracellular polymeric substances with polymeric ultrafiltration membranes." Journal of Membrane Science 309, no. 1-2 (2008): 28–35. http://dx.doi.org/10.1016/j.memsci.2007.10.012.

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9

Yu, Guang-Hui, Pin-Jing He, Li-Ming Shao, Duu-Jong Lee, and Arun S. Mujumdar. "Extracellular Polymeric Substances (EPS) and Extracellular Enzymes in Aerobic Granules." Drying Technology 28, no. 7 (2010): 910–15. http://dx.doi.org/10.1080/07373937.2010.490766.

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10

Cui, You-Wei, Yun-Peng Shi, and Xiao-Yu Gong. "Effects of C/N in the substrate on the simultaneous production of polyhydroxyalkanoates and extracellular polymeric substances by Haloferax mediterranei via kinetic model analysis." RSC Advances 7, no. 31 (2017): 18953–61. http://dx.doi.org/10.1039/c7ra02131c.

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11

Kumar Singha, Tapan. "Microbial Extracellular Polymeric Substances: Production, Isolation and Applications." IOSR Journal of Pharmacy (IOSRPHR) 2, no. 2 (2012): 276–81. http://dx.doi.org/10.9790/3013-0220276281.

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12

Flemming, H. C., T. R. Neu, and J. Wingender. "The Perfect Slime: Microbial Extracellular Polymeric Substances (EPS)." Water Intelligence Online 15 (August 18, 2016): 9781780407425. http://dx.doi.org/10.2166/9781780407425.

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13

Xiao, Yong, and Feng Zhao. "Electrochemical roles of extracellular polymeric substances in biofilms." Current Opinion in Electrochemistry 4, no. 1 (2017): 206–11. http://dx.doi.org/10.1016/j.coelec.2017.09.016.

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14

Liu, Hong, and Herbert H. P. Fang. "Extraction of extracellular polymeric substances (EPS) of sludges." Journal of Biotechnology 95, no. 3 (2002): 249–56. http://dx.doi.org/10.1016/s0168-1656(02)00025-1.

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15

Yu, Qiang, and Jeremy B. Fein. "Sulfhydryl Binding Sites within Bacterial Extracellular Polymeric Substances." Environmental Science & Technology 50, no. 11 (2016): 5498–505. http://dx.doi.org/10.1021/acs.est.6b00347.

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16

Zhang, Xiaoqi, and Paul L. Bishop. "Spatial Distribution of Extracellular Polymeric Substances in Biofilms." Journal of Environmental Engineering 127, no. 9 (2001): 850–56. http://dx.doi.org/10.1061/(asce)0733-9372(2001)127:9(850).

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17

Chen, M. Y., D. J. Lee, and J. H. Tay. "Distribution of extracellular polymeric substances in aerobic granules." Applied Microbiology and Biotechnology 73, no. 6 (2007): 1463–69. http://dx.doi.org/10.1007/s00253-006-0617-x.

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18

Suh, J. H., J. W. Yun, and D. S. Kim. "Effect of extracellular polymeric substances (EPS) on Pb." Bioprocess Engineering 21, no. 1 (1999): 1. http://dx.doi.org/10.1007/s004490050631.

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19

Peng, Meng, Jiayu Xu, Guang Yang, and Hongzhang Xu. "Digestion Properties of Intracellular Polymers and Extracellular Polymeric Substances and Influences of Extracellular Polymeric Substances on Anaerobic Digestion of Sludge." Journal of Environmental Engineering 146, no. 10 (2020): 04020112. http://dx.doi.org/10.1061/(asce)ee.1943-7870.0001787.

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20

Xiao, Yong, Enhua Zhang, Jingdong Zhang, et al. "Extracellular polymeric substances are transient media for microbial extracellular electron transfer." Science Advances 3, no. 7 (2017): e1700623. http://dx.doi.org/10.1126/sciadv.1700623.

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21

Xia, Peng-Fei, Qian Li, Lin-Rui Tan, Xue-Fei Sun, Chao Song, and Shu-Guang Wang. "Extracellular polymeric substances protect Escherichia coli from organic solvents." RSC Advances 6, no. 64 (2016): 59438–44. http://dx.doi.org/10.1039/c6ra11707d.

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22

Watanabe, Cláudia Hitomi, Rute Ferreira Domingos, Marc Fabien Benedetti, and André Henrique Rosa. "Dissolution and fate of silver nanoparticles in the presence of natural aquatic organic matter." Journal of Environmental Exposure Assessment 2, no. 1 (2023): 6. http://dx.doi.org/10.20517/jeea.2022.24.

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Despite increasing interest in and use of nanoparticles (NP), the environmental consequences of using NP are poorly understood because most relevant studies have not taken the effects of natural coatings on NP into consideration. The aim of this study was to improve our understanding of the fates of NP in aquatic systems. The fates of silver NP (AgNP) capped with citrate and polyethylene glycol dispersed in ecotoxicological matrices in the presence of environmentally relevant components of natural water (humic substances and extracellular polymeric substances) were investigated. Interactions b
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23

Yang, Yi, Shimei Zheng, Ruixuan Li, et al. "New insights into the facilitated dissolution and sulfidation of silver nanoparticles under simulated sunlight irradiation in aquatic environments by extracellular polymeric substances." Environmental Science: Nano 8, no. 3 (2021): 748–57. http://dx.doi.org/10.1039/d0en01142h.

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24

Wei, Shuyin, Feng Zeng, Yingyue Zhou, et al. "Phototransformation of extracellular polymeric substances in activated sludge and their interaction with microplastics." RSC Advances 13, no. 38 (2023): 26574–80. http://dx.doi.org/10.1039/d3ra04027e.

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25

Gopalakrishnan, Kishore, and Donna R. Kashian. "Extracellular polymeric substances in green alga facilitate microplastic deposition." Chemosphere 286 (January 2022): 131814. http://dx.doi.org/10.1016/j.chemosphere.2021.131814.

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26

Zhu, Liang, Haitian Yu, Yimei Liu, Hanying Qi, and Xiangyang Xu. "Optimization for extracellular polymeric substances extraction of microbial aggregates." Water Science and Technology 71, no. 7 (2015): 1106–12. http://dx.doi.org/10.2166/wst.2015.043.

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The extracellular polymeric substances (EPS) are important macromolecular components in microbial aggregates. The three EPS extraction methods – ultrasound + cation exchange resins (CER) + sulfide, ultrasound + formamide + NaOH, and ultrasound + heat – were investigated in the study, and the component differences of extracted EPS from the loose flocs and dense aerobic granules were compared using chemical analysis and three-dimensional excitation-emission matrix (3D-EEM). Results showed that the contents of EPS were extracted effectively by ultrasound + formamide + NaOH and ultrasound + heat m
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27

Jiao, Yongqin, George D. Cody, Anna K. Harding, et al. "Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms." Applied and Environmental Microbiology 76, no. 9 (2010): 2916–22. http://dx.doi.org/10.1128/aem.02289-09.

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ABSTRACT We examined the chemical composition of extracellular polymeric substances (EPS) extracted from two natural microbial pellicle biofilms growing on acid mine drainage (AMD) solutions. The EPS obtained from a mid-developmental-stage biofilm (DS1) and a mature biofilm (DS2) were qualitatively and quantitatively compared. More than twice as much EPS was derived from DS2 as from DS1 (approximately 340 and 150 mg of EPS per g [dry weight] for DS2 and DS1, respectively). Composition analyses indicated the presence of carbohydrates, metals, proteins, and minor quantities of DNA and lipids, al
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28

Adav, Sunil S., Duu-Jong Lee, and Joo-Hwa Tay. "Extracellular polymeric substances and structural stability of aerobic granule." Water Research 42, no. 6-7 (2008): 1644–50. http://dx.doi.org/10.1016/j.watres.2007.10.013.

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29

Yu, Han-Qing. "Molecular Insights into Extracellular Polymeric Substances in Activated Sludge." Environmental Science & Technology 54, no. 13 (2020): 7742–50. http://dx.doi.org/10.1021/acs.est.0c00850.

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30

Gerbersdorf, Sabine Ulrike, Bernhard Westrich, and David M. Paterson. "Microbial Extracellular Polymeric Substances (EPS) in Fresh Water Sediments." Microbial Ecology 58, no. 2 (2009): 334–49. http://dx.doi.org/10.1007/s00248-009-9498-8.

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31

Perkins, R. G., D. M. Paterson, H. Sun, J. Watson, and M. A. Player. "Extracellular polymeric substances: quantification and use in erosion experiments." Continental Shelf Research 24, no. 15 (2004): 1623–35. http://dx.doi.org/10.1016/j.csr.2004.06.001.

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32

Tourney, Janette, and Bryne T. Ngwenya. "The role of bacterial extracellular polymeric substances in geomicrobiology." Chemical Geology 386 (October 2014): 115–32. http://dx.doi.org/10.1016/j.chemgeo.2014.08.011.

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33

Lee, Chun-Chi, Duu-Jong Lee, and Juin-Yih Lai. "Labeling enzymes and extracellular polymeric substances in aerobic granules." Journal of the Taiwan Institute of Chemical Engineers 40, no. 5 (2009): 505–10. http://dx.doi.org/10.1016/j.jtice.2009.04.002.

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34

Ramirez-Mora, Tatiana, Cristina Retana-Lobo, and Grettel Valle-Bourrouet. "Biochemical characterization of extracellular polymeric substances from endodontic biofilms." PLOS ONE 13, no. 11 (2018): e0204081. http://dx.doi.org/10.1371/journal.pone.0204081.

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35

Khandeparker, Rakhee DS, and Narayan B. Bhosle. "Extracellular polymeric substances of the marine fouling diatomamphora rostrataWm.Sm." Biofouling 17, no. 2 (2001): 117–27. http://dx.doi.org/10.1080/08927010109378471.

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36

Wang, Zhi-Wu, Yu Liu, and Joo-Hwa Tay. "Biodegradability of extracellular polymeric substances produced by aerobic granules." Applied Microbiology and Biotechnology 74, no. 2 (2007): 462–66. http://dx.doi.org/10.1007/s00253-006-0686-x.

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37

Chen, Ming-Yuan, Duu-Jong Lee, Joo-Hwa Tay, and Kuan-Yeow Show. "Staining of extracellular polymeric substances and cells in bioaggregates." Applied Microbiology and Biotechnology 75, no. 2 (2007): 467–74. http://dx.doi.org/10.1007/s00253-006-0816-5.

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38

Sayahi, Naima, Bouthaina Othmani, Wissem Mnif, Zaina Algarni, Moncef Khadhraoui, and Faouzi Ben Rebah. "Microbial Extracellular Polymeric Substances as Corrosion Inhibitors: A Review." Surfaces 8, no. 3 (2025): 49. https://doi.org/10.3390/surfaces8030049.

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Microbial extracellular polymeric substances (EPSs) are emerging as sustainable alternatives to conventional corrosion inhibitors due to their eco-friendly nature, biodegradability, and functional versatility. Secreted by diverse microorganisms including bacteria, fungi, archaea, and algae, EPSs are composed mainly of polysaccharides, proteins, lipids, and nucleic acids. These biopolymers, chiefly polysaccharides and proteins, are accountable for surface corrosion prevention through biofilm formation, allowing microbial survival and promoting their environmental adaptation. Usually, EPS-mediat
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39

Wolcott, R. "Disrupting the biofilm matrix improves wound healing outcomes." Journal of Wound Care 24, no. 8 (2015): 366–71. http://dx.doi.org/10.12968/jowc.2015.24.8.366.

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Objective: The most unyielding molecular component of biofilm communities is the matrix structure that it can create around the individual microbes that constitute the biofilm. The type of polymeric substances (polymeric sugars, bacterial proteins, bacterial DNA and even co-opted host substances) are dependent on the microbial species present within the biofilm. The extracellular polymeric substances that make up the matrix give the wound biofilm incredible colony defences against host immunity, host healing and wound care treatments. This polymeric slime layer, which is secreted by bacteria,
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40

Zhang, Wan You, Xin Yan Wang, and Li Juan Xi. "Effect of Extracellular Polymeric Substances on Operation of Membrane Bioreactor." Advanced Materials Research 549 (July 2012): 491–95. http://dx.doi.org/10.4028/www.scientific.net/amr.549.491.

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In order to study the relationship between extracellular polymeric substances (EPS) and membrane fouling, the effect of extracellular polymeric substances (EPS) on the operation of membrane bioreactor (MBR) was investigated in this paper. The operation of membrane was analyzed by evaluating sludge volume index (SVI), modified fouling index (MFI), and membrane resistance (Rt), respectively. The results showed that SVI, MFI, and Rt increased with the accumulation of EPS, and membrane fouling aggravated with the increase of EPS, this illustrated that the content of EPS had a direct influence on S
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41

Bao, Peng, Mingchen Xia, Ajuan Liu, et al. "Extracellular polymeric substances (EPS) secreted byPurpureocillium lilacinumstrain Y3 promote biosynthesis of jarosite." RSC Advances 8, no. 40 (2018): 22635–42. http://dx.doi.org/10.1039/c8ra03060j.

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42

Elhadidy, Ahmed M., Michele I. Van Dyke, Fei Chen, Sigrid Peldszus, and Peter M. Huck. "Development and application of an improved protocol to characterize biofilms in biologically active drinking water filters." Environmental Science: Water Research & Technology 3, no. 2 (2017): 249–61. http://dx.doi.org/10.1039/c6ew00279j.

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43

Xu, Rui, Zhaohui Yang, Ting Chen, et al. "Anaerobic co-digestion of municipal wastewater sludge with food waste with different fat, oil, and grease contents: study of reactor performance and extracellular polymeric substances." RSC Advances 5, no. 125 (2015): 103547–56. http://dx.doi.org/10.1039/c5ra21459a.

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44

Palomares-Navarro, Julian J., Ariadna T. Bernal-Mercado, Gustavo A. González-Aguilar, Luis A. Ortega-Ramirez, Miguel A. Martínez-Téllez, and Jesús F. Ayala-Zavala. "Antibiofilm Action of Plant Terpenes in Salmonella Strains: Potential Inhibitors of the Synthesis of Extracellular Polymeric Substances." Pathogens 12, no. 1 (2022): 35. http://dx.doi.org/10.3390/pathogens12010035.

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Salmonella can form biofilms that contribute to its resistance in food processing environments. Biofilms are a dense population of cells that adhere to the surface, creating a matrix composed of extracellular polymeric substances (EPS) consisting mainly of polysaccharides, proteins, and eDNA. Remarkably, the secreted substances, including cellulose, curli, and colanic acid, act as protective barriers for Salmonella and contribute to its resistance and persistence when exposed to disinfectants. Conventional treatments are mostly ineffective in controlling this problem; therefore, exploring anti
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45

Huang, Xuquan, Jun Wang, Fei Xue, et al. "Investigating the Dewatering Efficiency of Sewage Sludge with Optimized Ratios of Electrolytic Manganese Residue Components." Materials 17, no. 14 (2024): 3605. http://dx.doi.org/10.3390/ma17143605.

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As an industrial waste residue, Electrolytic Manganese Residue (EMR) can greatly promote sludge dewatering and further particle-size optimization can significantly strengthen sludge dewaterability. In this study, the effects of ammonium sulfate, calcium sulphate dihydrate, and manganese carbonate in EMR on sludge dewatering performance were investigated using the response surface optimization method. It was found that the optimized ratio of three components in EMR was 1.0:1.6:2.2 based on capillary suction time (CST), specific resistance of filtration (SRF), and zeta potential of dewatered slu
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46

Arregui, Lucía, María Linares, Blanca Pέrez-Uz, Almudena Guinea, and Susana Serrano. "Involvement of Crawling and Attached Ciliates in the Aggregation of Particles in Wastewater Treatment Plants." Air, Soil and Water Research 1 (January 2008): ASWR.S752. http://dx.doi.org/10.4137/aswr.s752.

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The biological community in activated sludge wastewater plants is organized within this ecosystem as bioaggregates or flocs, in which the biotic component is embedded in a complex matrix comprised of extracellular polymeric substances mainly of microbial origin. The aim of this work is to study the role of different floc-associated ciliates commonly reported in wastewater treatment plants-crawling Euplotes and sessile Vorticella- in the formation of aggregates. Flocs, in experiments with ciliates and latex beads, showed more compactation and cohesion among particles than those in the absence o
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47

Shen, Li, Ran Chen, Junjun Wang, et al. "Biosorption behavior and mechanism of cadmium from aqueous solutions by Synechocystis sp. PCC6803." RSC Advances 11, no. 30 (2021): 18637–50. http://dx.doi.org/10.1039/d1ra02366g.

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The results of extracellular polymeric substances (EPS) extraction, physiological and biochemical determination and gene expression revealed the adsorption mechanism of Synechocystis sp. PCC6803 under cadmium stress.
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48

Peng, Jing, Kaili Wen, Wenzong Liu, Xiuping Yue, Aijie Wang, and Aijuan Zhou. "EPS solubilization and waste activated sludge acidification enhanced by alkaline-assisted bi-frequency ultrasonic pretreatment revealed by 3D-EEM fluorescence." RSC Advances 6, no. 84 (2016): 80493–500. http://dx.doi.org/10.1039/c6ra19521k.

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The effect of alkaline-assisted bi-frequency (28 + 40 kHz) ultrasonic pretreatment on extracellular polymeric substances (EPS) solubilization and waste activated sludge (WAS) acidification was investigated.
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49

Liu, Ying, Wenzhou Lv, Zhiqiang Zhang, and Siqing Xia. "Influencing characteristics of short-time aerobic digestion on spatial distribution and adsorption capacity of extracellular polymeric substances in waste activated sludge." RSC Advances 8, no. 56 (2018): 32172–77. http://dx.doi.org/10.1039/c8ra06277c.

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The spatial distribution and adsorption capacity of extracellular polymeric substances (EPS) were systematically investigated for waste activated sludge (WAS) treated by a short-time aerobic digestion (STAD) process.
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50

Zhang, Hongyu, Xuecheng Zheng, and Dongmin Lai. "Analysis of the Effects of Surfactants on Extracellular Polymeric Substances." Processes 11, no. 11 (2023): 3212. http://dx.doi.org/10.3390/pr11113212.

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Reservoirs after chemical flooding usually have residual chemicals, which can affect the driving effect of subsequent microbial drives. Among them, the effect of surfactants on the metabolites of oil-recovering bacteria is the most obvious. Therefore, this paper investigates the influence mechanism of sodium dodecyl sulfate (SDS) on the nature and structure of Extracellular Polymeric Substances (EPS) produced by metabolism of Enterobacter cloacae, through a variety of characterization to analysis the components and structure of EPS under SDS stress. The results showed that Enterobacter cloacae
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