Relevant bibliographies by topics / Extracellular recording

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Extracellular recording'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Extracellular recording"

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Du, Jiangang, Ingmar H. Riedel-Kruse, Janna C. Nawroth, Michael L. Roukes, Gilles Laurent, and Sotiris C. Masmanidis. "High-Resolution Three-Dimensional Extracellular Recording of Neuronal Activity With Microfabricated Electrode Arrays." Journal of Neurophysiology 101, no. 3 (March 2009): 1671–78. http://dx.doi.org/10.1152/jn.90992.2008.

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Microelectrode array recordings of neuronal activity present significant opportunities for studying the brain with single-cell and spike-time precision. However, challenges in device manufacturing constrain dense multisite recordings to two spatial dimensions, whereas access to the three-dimensional (3D) structure of many brain regions appears to remain a challenge. To overcome this limitation, we present two novel recording modalities of silicon-based devices aimed at establishing 3D functionality. First, we fabricated a dual-side electrode array by patterning recording sites on both the front and back of an implantable microstructure. We found that the majority of single-unit spikes could not be simultaneously detected from both sides, suggesting that in addition to providing higher spatial resolution measurements than that of single-side devices, dual-side arrays also lead to increased recording yield. Second, we obtained recordings along three principal directions with a multilayer array and demonstrated 3D spike source localization within the enclosed measurement space. The large-scale integration of such dual-side and multilayer arrays is expected to provide massively parallel recording capabilities in the brain.
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Allen, Brian D., Caroline Moore-Kochlacs, Jacob G. Bernstein, Justin P. Kinney, Jorg Scholvin, Luís F. Seoane, Chris Chronopoulos, et al. "Automated in vivo patch-clamp evaluation of extracellular multielectrode array spike recording capability." Journal of Neurophysiology 120, no. 5 (November 1, 2018): 2182–200. http://dx.doi.org/10.1152/jn.00650.2017.

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Much innovation is currently aimed at improving the number, density, and geometry of electrodes on extracellular multielectrode arrays for in vivo recording of neural activity in the mammalian brain. To choose a multielectrode array configuration for a given neuroscience purpose, or to reveal design principles of future multielectrode arrays, it would be useful to have a systematic way of evaluating the spike recording capability of such arrays. We describe an automated system that performs robotic patch-clamp recording of a neuron being simultaneously recorded via an extracellular multielectrode array. By recording a patch-clamp data set from a neuron while acquiring extracellular recordings from the same neuron, we can evaluate how well the extracellular multielectrode array captures the spiking information from that neuron. To demonstrate the utility of our system, we show that it can provide data from the mammalian cortex to evaluate how the spike sorting performance of a close-packed extracellular multielectrode array is affected by bursting, which alters the shape and amplitude of spikes in a train. We also introduce an algorithmic framework to help evaluate how the number of electrodes in a multielectrode array affects spike sorting, examining how adding more electrodes yields data that can be spike sorted more easily. Our automated methodology may thus help with the evaluation of new electrode designs and configurations, providing empirical guidance on the kinds of electrodes that will be optimal for different brain regions, cell types, and species, for improving the accuracy of spike sorting. NEW & NOTEWORTHY We present an automated strategy for evaluating the spike recording performance of an extracellular multielectrode array, by enabling simultaneous recording of a neuron with both such an array and with patch clamp. We use our robot and accompanying algorithms to evaluate the performance of multielectrode arrays on supporting spike sorting.
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Piironen, Arto, Matti Weckström, and Mikko Vähäsöyrinki. "Ultrasmall and customizable multichannel electrodes for extracellular recordings." Journal of Neurophysiology 105, no. 3 (March 2011): 1416–21. http://dx.doi.org/10.1152/jn.00790.2010.

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Increasing demand exists for smaller multichannel electrodes that enable simultaneous recordings of many neurons in a noninvasive manner. We report a novel method for manufacturing ultrasmall carbon fiber electrodes with up to seven closely spaced recording sites. The electrodes were designed to minimize damage to neuronal circuitry and to be fully customizable in three dimensions so that their dimensions can be optimally matched to those of the targeted neuron population.
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Huizinga, Jan D. "The powerful advantages of extracellular electrical recording." Nature Reviews Gastroenterology & Hepatology 14, no. 6 (March 30, 2017): 372. http://dx.doi.org/10.1038/nrgastro.2017.16.

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Hai, Aviad, Joseph Shappir, and Micha E. Spira. "Long-Term, Multisite, Parallel, In-Cell Recording and Stimulation by an Array of Extracellular Microelectrodes." Journal of Neurophysiology 104, no. 1 (July 2010): 559–68. http://dx.doi.org/10.1152/jn.00265.2010.

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Here we report on the development of a novel neuroelectronic interface consisting of an array of noninvasive gold-mushroom-shaped microelectrodes (gMμEs) that practically provide intracellular recordings and stimulation of many individual neurons, while the electrodes maintain an extracellular position. The development of this interface allows simultaneous, multisite, long-term recordings of action potentials and subthreshold potentials with matching quality and signal-to-noise ratio of conventional intracellular sharp glass microelectrodes or patch electrodes. We refer to the novel approach as “in-cell recording and stimulation by extracellular electrodes” to differentiate it from the classical intracellular recording and stimulation methods. This novel technique is expected to revolutionize the analysis of neuronal networks in relations to learning, information storage and can be used to develop novel drugs as well as high fidelity neural prosthetics and brain-machine systems.
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Cohen, M. L., R. H. Hoyt, J. E. Saffitz, and P. B. Corr. "A high density in vitro extracellular electrode array: description and implementation." American Journal of Physiology-Heart and Circulatory Physiology 257, no. 2 (August 1, 1989): H681—H689. http://dx.doi.org/10.1152/ajpheart.1989.257.2.h681.

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The detailed activation sequence in myocardium provides information critical to the understanding of the mechanisms of cardiac arrhythmias and the influence of interventions. Despite the pivotal role of activation mapping, the interpretation of isochronic maps and the correlation to detailed tissue morphology may be limited when the interelectrode distances are large with respect to cell dimensions. Additionally, dynamic beat-to-beat variations in the activation pattern or the effect of interventions such as single extra stimuli cannot be assessed adequately without recording from all sites simultaneously. To surmount these limitations, we have fabricated and tested an extracellular recording array consisting of 224 bipolar tungsten wire electrodes with a 350-microns interelectrode distance (140 microns edge-to-edge distance), and used signal processing equipment to record from all electrodes simultaneously at a 2-kHz sample rate. Stimulation can be performed sequentially from 12 different sites at 30 degree angles around the periphery of the recording array. Transarray bipoles can be recorded from any combination of eight radially oriented sites. Activation maps recorded in normal tissue after programmed stimulation and activation maps from an area of fixed anatomic block in the epicardial border zone of infarcted tissue are presented. The results demonstrate a lack of influence of the recording array on the electrophysiological properties of the tissue as verified with transmembrane action potential recordings and sequential extracellular maps. This electrode permits precise assessment of transient details of the activation sequence with unparalleled anatomic resolution.
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Liu, Xinyu, Hong Wan, and Li Shi. "Quality Metrics of Spike Sorting Using Neighborhood Components Analysis." Open Biomedical Engineering Journal 8, no. 1 (September 17, 2014): 60–67. http://dx.doi.org/10.2174/1874120701408010060.

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While an electrode has allowed for simultaneously recording the activity of many neurons in microelectrode extracellular recording techniques, quantitative metrics of cluster quality after sorting to identify clusters suited for single unit analysis are lacking. In this paper, an objective measure based on the idea of neighborhood component analysis was described for evaluating cluster quality of spikes. The proposed method was tested with experimental and simulated extracellular recordings as well as compared to isolation distance and Lratio. The results of simulation and real data from the rodent primary visual cortex have shown that values of the proposed method were related to the accuracy of spike sorting, which could discriminate well- and poorly-separated clusters. It can apply on any study based on the activity of single neurons.
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Camuñas-Mesa, Luis A., and Rodrigo Quian Quiroga. "A Detailed and Fast Model of Extracellular Recordings." Neural Computation 25, no. 5 (May 2013): 1191–212. http://dx.doi.org/10.1162/neco_a_00433.

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We present a novel method to generate realistic simulations of extracellular recordings. The simulations were obtained by superimposing the activity of neurons placed randomly in a cube of brain tissue. Detailed models of individual neurons were used to reproduce the extracellular action potentials of close-by neurons. To reduce the computational load, the contributions of neurons further away were simulated using previously recorded spikes with their amplitude normalized by the distance to the recording electrode. For making the simulations more realistic, we also considered a model of a finite-size electrode by averaging the potential along the electrode surface and modeling the electrode-tissue interface with a capacitive filter. This model allowed studying the effect of the electrode diameter on the quality of the recordings and how it affects the number of identified neurons after spike sorting. Given that not all neurons are active at a time, we also generated simulations with different ratios of active neurons and estimated the ratio that matches the signal-to-noise values observed in real data. Finally, we used the model to simulate tetrode recordings.
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Ioffe, S., A. H. Jansen, and V. Chernick. "Technique for repetitive recording from fetal respiratory neurons." Journal of Applied Physiology 80, no. 3 (March 1, 1996): 1057–60. http://dx.doi.org/10.1152/jappl.1996.80.3.1057.

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We developed a new method for repetitive recording of medullary neurons in fetal sheep in situ. The technique involves chronically fixing the fetal head to the flank of the ewe by way of a Teflon plate that has a removable window. This window allows direct access of a recording electrode to the floor of the fourth ventricle of the fetus. In four of six fetuses, repetitive recordings lasting 3-4 h were possible for up to 6 days. By operating on younger fetuses and with care, this time span could be extended. This novel method should be useful in the future for extracellular and intracellular recordings of neurons in the developing fetus without disturbing the fetal state and for the study of putative neurotransmitters during development with iontophoretic techniques.
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Tokuno, Hironobu, Yoko Ikeuchi, Atsushi Nambu, Toshikazu Akazawa, Michiko Imanishi, Ikuma Hamada, and Naomi Hasegawa. "A modified microsyringe for extracellular recording of neuronal activity." Neuroscience Research 31, no. 3 (July 1998): 251–55. http://dx.doi.org/10.1016/s0168-0102(98)00041-8.

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Dissertations / Theses on the topic "Extracellular recording"

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Blum, Richard Alan. "An Electronic System for Extracellular Neural Stimulation and Recording." Diss., Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/16192.

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A system for extracellular neural interfacing that had the capability for stimulation and recording at multiple electrodes was presented. As the core of this system was a custom integrated circuit (IC) that contained low-noise amplifiers, stimulation buffers, and artifact-elimination circuitry. The artifact-elimination circuitry was necessary to prevent the activity of the stimulation buffers from interfering with the normal functioning of the low-noise amplifiers. The integrated circuits were fabricated in in a 0.35 micron CMOS process. We measured input-referred noise levels for the amplifiers as low as 3.50 microvolts (rms) in the in the bandwidth 30 Hz-3 kHz, corresponding to the frequency range of neural action potentials. The power consumption was 120 microwatts, corresponding to a noise-efficiency factor of 14.5. It was possible to resume recording signals within 2 ms of a stimulation, using the same electrode for both stimulation and recording. A filtering algorithm to remove the post-discharge artifact was also presented. The filtering was implemented using a field-programmable gate array (FPGA). The filtering algorithm itself consisted of blanking for the duration of the stimulation and artifact-elimination, followed by a wavelet de-noising. The wavelet de-noising split the signal into frequency ranges, discarded those ranges that did not correspond to neural signals, applied a threshold to the retained signals, and recombined the different frequency ranges into a single signal. The combination of the filtering with the artifact-elimination IC resulted in the capability for artifact-free recordings.
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Bernstein, Jacob (Jacob Gold). "Development of extracellular electrophysiology methods for scalable neural recording." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/107581.

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Thesis: Ph. D., Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2016.
Cataloged from PDF version of thesis.
Includes bibliographical references.
In order to map the dynamics of neural circuits in mammalian brains, there is a need for tools that can record activity over large volumes of tissue and correctly attribute the recorded signals to the individual neurons that generated them. High-resolution neural activity maps will be critical for the discovery of new principles of neural coding and neural computation, and to test computational models of neural circuits. Extracellular electrophysiology is a neural recording method that has been developed to record from large populations of neurons, but well-known problems with signal attribution pose an existential threat to the viability of further system scaling, as analyses of network function become more sensitive to errors in attribution. A key insight is that blind-source separation algorithms such as Independent Component Analysis may ameliorate problems with signal attribution. These algorithms require recording signals at much finer spatial resolutions than existing probes have accomplished, which places demands on recording system bandwidth. We present several advances to technologies in neural recording systems, and a complete neural recording system designed to investigate the challenges of scaling electrophysiology to whole brain recording. We have developed close-packed microelectrode arrays with the highest density of recording sites yet achieved, for which we built our own data acquisition hardware, developed with a computational architecture specifically designed to scale to over several orders of magnitude. We also present results from validation experiments using colocalized patch clamp recording to obtain ground-truth activity data. This dataset provides immediate insight into the nature of electrophysiological signals and the interpretation of data collected from any electrophysiology recording system. This data is also essential in order to optimize probe development and data analysis algorithms which will one day enable whole-brain activity mapping.
by Jacob G. Bernstein.
Ph. D.
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Sayed, Herbawi Abdalrahman [Verfasser], and Oliver [Akademischer Betreuer] Paul. "High-density CMOS probes for large-scale extracellular neural recording." Freiburg : Universität, 2020. http://d-nb.info/1226657265/34.

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Kuykendal, Michelle Lea. "Closed-loop optimization of extracellular electrical stimulation for targeted neuronal activation." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/52303.

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We have developed a high-throughput system of closed-loop electrical stimulation and optical recording that facilitates the rapid characterization of extracellular stimulus-evoked neural activity. The ability to selectively stimulate a neuron is a defining characteristic of next-generation neural prostheses. Greater stimulus control and differential activation of specific neuronal populations allows for prostheses that better mimic their biological counterparts. In our system, we deliver square current pulses using a microelectrode array; automated real-time image processing of high-speed digital video identifies the neuronal response; and a feedback controller alters the applied stimulus to achieve a targeted response. The system controller performs directed searches within the strength-duration (SD) stimulus parameter space to build probabilistic neuronal activation curves. An important feature of this closed-loop system is a reduction in the number of stimuli needed to derive the activation curves when compared to the more commonly used open-loop system: this allows the closed-loop system to spend more time probing stimulus regions of interest in the multi-parameter waveform space, facilitating high resolution analysis. The stimulus-evoked activation data were well-fit to a sigmoid model in both the stimulus strength (current) and duration (pulse width) slices through the waveform space. The 2-D analysis produced a set of probability isoclines corresponding to each neuron-electrode pairing, which were fit to the SD threshold model described by Lapique (1907). We show that stimulus selectivity within a given neuron pair is possible in the one-parameter search space by using multiple stimulation electrodes. Additionally, by applying simultaneous stimuli to adjacent electrodes, the interaction between stimuli alters the neuronal activation threshold. The interaction between simultaneous multi-electrode multi-parameter stimulus waveforms creates an opportunity for increased stimulus selectivity within a population. We demonstrated that closed-loop imaging and micro-stimulation technology enable the study of neuronal excitation across a large parameter space, which is requisite for controlling neuronal activation in next generation clinical solutions.
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Czeschik, Anna [Verfasser], Bernhard [Akademischer Betreuer] Wolfrum, and Jörg [Akademischer Betreuer] Fitter. "Nanocavity arrays for extracellular recording and stimulation of electroactive cell systems / Anna Czeschik ; Bernhard Wolfrum, Jörg Fitter." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/1130151530/34.

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Patel, Manoj Kumar. "An investigation into electrophysiological changes associated with myocardial ischaemia and reperfusion using extracellular and intracellular recording techniques." Thesis, Coventry University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308953.

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Sörensen, Sören Per. "Development of a cell-based drug screening platform : extracellular recording and electrochemical impedance spectroscopy on microelectrode array chips." Thesis, University of Bath, 2007. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.486476.

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Two established methods, Electrochemical Impedance Spectroscopy (EIS) and extracellular recording, were implemented into a technology platform for non-invasive whole-cell biosensing. Electrical activity of cardiomyocytes and cell-substrate interaction of human ovarian cancer cells was monitored on electrode array chips. The performance of cells inside a microfluidic or closed low volume environment was investigated. Prior to the development of the entire microfluidic platform the two transducing methods were evaluated in single experiments. Processes as cellular attachment and detachment were monitored using EIS and single frequency impedance sensing. Electrodes of different size and structure were employed and compared for their impedance response. It was shown that small electrodes (A = 9·10-6 cm²) are more sensitive to cell-substrate interaction than larger ones (A = 9·10-5 cm²) and that the frequency used for analysis has a profound influence on the sensitivity. Data were modelled using a common equivalent circuit that represents a cell layer on an electrode resulting in an increase of the impedance magnitude by <170 % due to cell attachment. In order to demonstrate the potential of this method for biomedical applications, experiments related to anti-cancer strategies were performed. Cell detachment was induced by addition of synthetic integrin ligands and by hypericin mediated photodynamic therapy and monitored with impedance-based biosensing. Electrical activity of cardiomyocytes cultured on microelectrode arrays was monitored inside a microfluidic system. The chronotropic drug isoproterenol was applied using a robotic dispensing machine, and the resulting changes in spike rate and duration were compared with results gained by experiments with a large scale MEA chip. The experimental findings inspired the development of a technology platform that was finally evaluated by monitoring extracellular signals from myocytes in response to Isoproterenol. Another topic was the comparison of cell-substrate interaction monitored on various electrode structures.
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Bradley, Peter Mark James. "A Novel Fibre-Optic Probe for Simultaneous Extracellular Electrical and Intracellular Fluorescence Recording in Neurones In Situ and In Vito." Thesis, University of Bristol, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503894.

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Steidl, Esther. "Mise au point d’une plateforme de tests in vitro pour l’évaluation du potentiel proconvulsivant de façon précoce au cours du développement de médicaments." Thesis, Aix-Marseille, 2019. http://www.theses.fr/2019AIXM0050.

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L’objectif des travaux de recherche présentés ici était la mise au point d’une plateforme de tests pour identifier le potentiel proconvulsivant de composés de façon précoce au cours de leur développement. Ces tests ont été réalisés in vitro à partir de tranches d’hippocampes enregistrées à l’aide de multi-electrode arrays (MEA). La technique du MEA est particulièrement adaptée pour ce type d’investigations car elle permet d’évaluer l’effet de composés sur une large partie d’un réseau neuronal natif. De plus, cette technique permet de réaliser des tests à moyen débit et à partir de faibles quantités de composé.Tout d’abord, l’évaluation de composés de référence a permis de déterminer les paramètres d’intérêt, qui sont modifiés par des composés proconvulsivants. Les composés proconvulsivants testés ont causé une augmentation de l’aire des potentiels d’action de population et l’apparition de potentiels d’action additionnels, déclenché des décharges épileptiformes et/ou augmenté la fréquence de décharge des neurones de la région CA1. Les conditions expérimentales des tests réalisés ont ensuite été modifiées pour augmenter leur sensibilité et permettre la détection de proconvulsivants faibles. Cette plateforme de 3 tests complémentaires a été nommée NS-PC set. Pour valider la plateforme NS-PC set, 15 composés fournis par des sociétés pharmaceutiques partenaires, incluant des contrôles positifs et négatifs, ont été testés à l’aveugle. Un nouveau type de test plus rapide et abordable appelé NS-PC screen a ensuite été mis au point, sur la base d’enregistrement de décharges épileptiformes induites par la 4-aminopyridine dans des tranches d’hippocampe
The goal of the present work was to develop a platform of tests that could predict the proconvulsive potential of compounds in development as early as possible during preclinical phases. These tests were carried out in vitro from hippocampal slices recorded with multi-electrode arrays (MEAs). The MEA technology is particularly adapted because it allows to investigate compounds’ effect on a wide area of a native neural network, including all the complexity and organization of the different cell types. In addition, rapidity and low compound consumption of the MEA-based assays make them suitable for early stages of development.First, the evaluation of reference proconvulsive/seizurogenic compounds allowed to determine the parameters that should be monitored to detect proconvulsive properties. It appeared that reference compounds triggered one or several of the following effects: increase of the population spikes area and repetition of spikes, triggering of epileptiform discharges and/or increase of the CA1 neurons firing. The experimental conditions of the assays were then modified to increase their sensitivity and thus detect even weak proconvulsive compounds. This platform of 3 complementary assays was termed NS-PC set.15 compounds, including positive and negative controls, were provided by partner pharmaceutical companies to be tested under blind conditions on NS-PC set. A new faster and cheaper assay, termed NS-PC screen, was also designed based on the recording of 4-aminopyridine-induced epileptiform discharges in hippocampal slices
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Krüger, Hagen. "Elektrophysiologische Untersuchungen zu Einflüssen von ionotropen Glutamatantagonisten sowie 5-HT1A-Agonisten auf die Kaliumchlorid-induzierte "spreading depression" im neokortikalen Hirnschnittpräparat der adulten Ratte." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2000. http://dx.doi.org/10.18452/14462.

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Die kortikale spreading depression (SD), wie sie von Leão 1944 zuerst beschrieben wurde, ist ein elektrophysiologisches Phänomen, das in der Pathophysiologie der Aurasymptomatik einer Mi-gräneattacke und Ischämie-induzierter Zellschäden diskutiert wird. Während der akuten fokalen zerebralen Ischämie treten eine Reihe von Ereignissen wie eine massive Entzündungsreaktion und die allmähliche Einbeziehung einer zunächst viablen ischämischen Randzone - der Penum-bra - in das infarzierte Hirngewebe auf. Da an diesen Ereignissen SD-ähnliche Depolarisationen kausal beteiligt sind, ist die pharmakologische Verringerung von SD-Episoden bzw. eine Ver-kleinerung ihrer Amplitude und Dauer unter in vitro als auch tierexperimentellen in vivo Bedin-gungen eine mögliche neuroprotektive Strategie. In der vorliegenden Arbeit wurde ein in vitro Modell beschrieben, das am Hirnschnittpräparat des Neokortex der adulten Ratte eine reproduzierbare Auslösung von SD-Wellen unter normoxi-schen Bedingungen gestattet. Anhand von charakteristischen elektrophysiologischen Parametern einer SD wie Amplitude, Dauer und Ausbreitungsgeschwindigkeit wurden die gute Überein-stimmungen dieses in vitro Modells mit in vivo Modellen gezeigt. Obwohl SD Wellen am nicht-ischämischen Kortex keine morphologischen Schäden verursachen, zeigte sich in den hier vorge-stellten Experimenten eine funktionelle Unterdrückung der GABAergen hemmenden Mechanis-men des Neokortex nach repetitiven SDs auch bei ausreichender Energie- und Sauerstoffversor-gung. Die hier diskutierten Ergebnisse demonstrierten, daß unter in vitro Bedingungen der AMPA-Glutamatrezeptor für die Auslösung und Ausbreitung einer SD eine untergeordnete Rolle spielt. Demgegenüber erwies sich die NMDA-Rezeptoraktivierung als herausragend für eine SD, da die Blockade dieses Rezeptors mit dem nicht-kompetitiven Antagonisten Ketamin die SD-Amplitude und SD-Dauer signifikant verringerte. Die Anwendung der selektiven 5-HT1A-Agonisten 8-OH-DPAT und BAY x 3702 erwies sich als eine neue Möglichkeit, die Zeitdauer einer SD zu verringern. Die aufgezeigte SD-induzierte neuronale Hyperexzitabilität kann unter normoxischen Bedingun-gen zelluläre Dysfunktionen verursachen und auch an einer Generierung der Aura eines Migrä-neanfalls beteiligt sein. Unter hypoxisch-ischämischen Bedingungen könnte eine SD-induzierte Dysfunktion GABAerger Kontrollmechanismen die Ausweitung ischämischer Zellschäden be-wirken. Die Hoffnungen auf eine effektive Schlaganfalltherapie haben sich mit den bisherigen NMDA-Antagonisten trotz ihrer hier bestätigten guten in vitro Wirksamkeit aufgrund der Interferenz mit physiologischen Glutamatfunktionen im Kortex nicht erfüllt. Die hier gezeigte konzentrationsab-hängige Verkürzung der SD-Dauer durch die Aktivierung des 5-HT1A-Serotoninrezeptors unter in vitro Bedingungen kann bei der bekannten hohen 5-HT1A-Rezeptor-mRNA-dichte an beson-ders ischämievulnerablen Neuronen einen neuen neuroprotektiven Ansatz auch beim Menschen darstellen. Weitere Untersuchungen müssen zeigen, ob die hier beschriebene enge Verflechtung des serotonergen Systems mit der glutamatergen Neurotransmission eventuell auch zu uner-wünschte Wirkungen unter in vivo Bedingungen führt.
Repetitive cortical spreading depression (SD) and SD-like events, associated with a massive de-polarization of neuronal and glial cells, is thought to play a key role in the induction of neuronal damage in the peri-infarct zone following experimental focal cerebral ischemia. In addition, ex-perimental and clinical data suggest that SD is the underlying mechanism of neurological distur-bances during migraine auras as well. However, detailed analyses on the consequences of repeti-tive SDs on cortical function and involved receptors are lacking. Using an in vitro rat model of SD I investigated in this thesis the electrophysiological properties of repetitive potassium chloride (KCl)-induced SDs, their influence on synaptic neurotransmis-sion and the effects of ionotropic glutamate antagonists and 5-HT1A agonists in neocortical slices obtained from adult rats. Whereas repetitive SDs revealed only non-significant variations in du-ration, amplitude and integral when elicited at intervals of 30 min, paired-pulse inhibition of ex-tracellularly recorded field potential responses was significantly affected by repetitive SD even under normoxic conditions. Compared to the control recordings, each SD episode caused a sig-nificant decrease in the efficacy of intracortical GABAergic inhibition by approximately 10%. Since excitatory synaptic transmission was unaffected, these data indicate that repetitive SDs cause a selective suppression of GABAergic function even in the non-ischemic brain. None of the compounds tested prevented the SD-induced cortical disinhibition. However, the SD-associated negative shift in the extracellular DC potential was reduced by ketamine, a selective N-methyl-D-aspartic acid (NMDA-) receptor antagonist. Ketamine significantly (p < 0.01) re-duced the amplitude of the first SD peak and blocked the second SD peak. Ketamine also de-creased the SD duration at half maximal amplitude (p < 0.05). NBQX, a selective a-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonist did not affect the SD-accompanied cortical depolarization, whereas selective 5-hydroxytryptamine (5-HT)1A receptor agonists 8-OH-DPAT and BAY x 3702 shortened concentration-dependently the duration of the SD up to 50 %. Nevertheless, both 5-HT1A receptor agonists caused a strong disinhibition of neu-ronal function with a tendency towards paired-pulse facilitation as well. Thus, repetitive SD and SD-like events may induce neuronal hyperexcitability due to a selective suppression of intrinsic inhibitory GABAergic function. Under normoxic conditions, SD-induced disinhibition may be involved in the generation and maintenance of migraine or associated neurological disturbances. Under hypoxic-ischemic conditions, neuronal hyperexcitability may contribute to the gradual expansion of the ischemic core and the metabolic deterioration of the penumbral tissue after SD episodes. This underlines the deleterious effect of SD to the outcome of focal cerebral ischemia. Although the precise mecha-nisms of SD generation and propagation remains far from established, the present pharmacologi-cal profile of KCl-induced SD in vitro links the induction and propagation of SD in rat neocorti-cal slices mainly to a local increase of [K + ] e and a subsequent activation of NMDA- receptors. This corroborates the neuroprotective effect of a NMDA- receptor blockade observed in various in vitro and in vivo models. However, as it has been demonstrated in clinical trials, NMDA- re-ceptor antagonists in use today cause psychomimetic and cardiovascular side effects in humans and are therefore currently of low clinical benefit. The activation of 5-HT1A receptors by selective agonists represents a new pharmacological strategy in the treatment of acute ischemic stroke, since shortened SD waves may represent a less energy-consuming process under conditions of limited energy supply and are probably associated with an efflux of excitatory neurotransmitters to a lesser extent. The potential clinical benefit of 5-HT 1A receptor agonists remains to be investi-gated in clinical trials, since systemic administration of these compounds after the onset of acute focal cerebral ischemia might interfere with normal functions of glutamatergic neurotransmission in the intact, non-ischemic brain.
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Books on the topic "Extracellular recording"

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Sillitoe, Roy V., ed. Extracellular Recording Approaches. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7549-5.

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Extracellular Recording Approaches. Humana, 2018.

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Coleman, William L., and R. Michael Burger. Extracellular Single-Unit Recording and Neuropharmacological Methods. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0003.

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Small biogenic changes in voltage such as action potentials in neurons can be monitored using extracellular single unit recording techniques. This technique allows for investigation of neuronal electrical activity in a manner that is not disruptive to the cell membrane, and individual neurons can be recorded from for extended periods of time. This chapter discusses the basic requirements for an extracellular recording setup, including different types of electrodes, apparatus for controlling electrode position and placement, recording equipment, signal output, data analysis, and the histological confirmation of recording sites usually required for in vivo recordings. A more advanced extracellular recording technique using piggy-back style multibarrel electrodes that allows for localized pharmacological manipulation of neuronal properties is described in detail. Strategies for successful signal isolation, troubleshooting advice such as noise reduction, and suggestions for general laboratory equipment are also discussed.
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Stegeman, Dick F., and Michel J. A. M. Van Putten. Recording of neural signals, neural activation, and signal processing. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199688395.003.0005.

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This chapter discusses recording of electrophysiological signals in the context of clinical neurophysiology. We first discuss the interpretation of signals and differences between signals in terms of their underlying (electro)physiology. As a most prominent aspect of applied electrophysiology, the biophysics of volume conduction in extracellular space is discussed. We also present some basics of advanced procedures to analyse neurophysiological data. Aspects of electrical stimulation are treated too, including recent developments in diagnostic and therapeutic constant current stimulation. We finally discuss the background of hazardous electric currents and the safety of bioelectric equipment. Aspects that are relevant in the digitization and post-processing of data are briefly reviewed.
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Book chapters on the topic "Extracellular recording"

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Ellenbroek, Bart, Alfonso Abizaid, Shimon Amir, Martina de Zwaan, Sarah Parylak, Pietro Cottone, Eric P. Zorrilla, et al. "Extracellular Recording." In Encyclopedia of Psychopharmacology, 522–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_290.

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Wakerley, Jon. "Extracellular Recording." In Essential Guide to Reading Biomedical Papers, 261–69. Chichester, UK: John Wiley & Sons, Ltd, 2012. http://dx.doi.org/10.1002/9781118402184.ch29.

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Harvey, Victoria L., and Anthony H. Dickenson. "Extracellular Recording." In Encyclopedia of Psychopharmacology, 1–5. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27772-6_290-2.

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Harvey, Victoria L., and Anthony H. Dickenson. "Extracellular Recording." In Encyclopedia of Psychopharmacology, 665–69. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-36172-2_290.

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Franco, J. C., M. A. Portela, and H. Andrade-Caicedo. "Multichannel Planar Microelectrode Platform for Recording Extracellular Field Potentials." In VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016, 642–45. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4086-3_161.

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Sokoloff, Greta, and Mark S. Blumberg. "Recording Extracellular Activity in the Developing Cerebellum of Behaving Rats." In Neuromethods, 225–47. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7549-5_12.

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Pezaris, John S., Maneesh Sahani, and Richard Andersen. "Extracellular Recording from Multiple Neighboring Cells: Response Properties in Parietal Cortex." In Computational Neuroscience, 483–89. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4831-7_80.

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Asgarifar, Sanaz, Henrique L. Gomes, Ana Mestre, Pedro Inácio, J. Bragança, Jérôme Borme, George Machado, Fátima Cerqueira, and Pedro Alpuim. "Electrochemically Gated Graphene Field-Effect Transistor for Extracellular Cell Signal Recording." In Technological Innovation for Cyber-Physical Systems, 558–64. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-31165-4_53.

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Heiney, Shane A., Shogo Ohmae, Olivia A. Kim, and Javier F. Medina. "Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice." In Neuromethods, 39–71. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7549-5_3.

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Graziane, Nicholas, and Yan Dong. "Extracellular Recordings." In Neuromethods, 249–57. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3274-0_22.

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Conference papers on the topic "Extracellular recording"

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Novak, D., J. Wild, T. Sieger, and R. Jech. "Identifying number of neurons in extracellular recording." In 2009 4th International IEEE/EMBS Conference on Neural Engineering (NER). IEEE, 2009. http://dx.doi.org/10.1109/ner.2009.5109403.

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Anderson, Wise, and Najafi. "Micromachined Silicon Substrate Electrodes For Extracellular Recording." In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1992. http://dx.doi.org/10.1109/iembs.1992.593825.

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Anderson, D. J., K. D. Wise, and K. Najafi. "Micromachined silicon substrate electrodes for extracellular recording." In 1992 14th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1992. http://dx.doi.org/10.1109/iembs.1992.5761722.

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Joye, Neil, Alexandre Schmid, and Yusuf Leblebici. "Extracellular recording system based on amplitude modulation for CMOS microelectrode arrays." In 2010 IEEE Biomedical Circuits and Systems Conference (BioCAS). IEEE, 2010. http://dx.doi.org/10.1109/biocas.2010.5709581.

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Yin Zhou and Zhi Yang. "A robust EC-PC spike detection method for extracellular neural recording." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6609756.

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Myers, F. B., O. J. Abilez, C. K. Zarins, and L. P. Lee. "Stimulation and artifact-free extracellular electrophysiological recording of cells in suspension." In 2011 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2011. http://dx.doi.org/10.1109/iembs.2011.6091001.

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Niemiec, Martin J., and Martin Han. "A Simple Table-Top Technique for Multi-Signal Pseudo-Extracellular Recording." In 2021 10th International IEEE/EMBS Conference on Neural Engineering (NER). IEEE, 2021. http://dx.doi.org/10.1109/ner49283.2021.9441208.

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Delgado-Restituto, Manuel, Alberto Rodriguez-Perez, Angela A. Darie, Angel Rodriguez-Vazquez, Cristina Soto-Sanchez, and Eduardo Fernandez-Jover. "In vivo measurements with a 64-channel extracellular neural recording integrated circuit." In 2014 21st IEEE International Conference on Electronics, Circuits and Systems (ICECS). IEEE, 2014. http://dx.doi.org/10.1109/icecs.2014.7050028.

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Wu, Chunsheng, Liping Du, Zhen Qin, Keqiang Gao, and Ping Wang. "Dual extracellular recording using a light-addressable potentiometric sensor for taste signal transduction." In 2017 ISOCS/IEEE International Symposium on Olfaction and Electronic Nose (ISOEN). IEEE, 2017. http://dx.doi.org/10.1109/isoen.2017.7968920.

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Shaik, Faruk A., Y. Ikeuchi, G. Cathcart, S. Ihida, H. Toshiyoshi, and A. Tixier-Mita. "Extracellular neural stimulation and recording with a Thin-Film-Transistor (TFT) array device." In 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS). IEEE, 2017. http://dx.doi.org/10.1109/transducers.2017.7994024.

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