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Journal articles on the topic 'Faecal samples'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Vulich, S. A., and J. P. Hanrahan. "Faecal sampling for the estimation of herbage intake using n-alkanes: evaluation of sample pooling and the use of rectal grab samples." Journal of Agricultural Science 124, no. 1 (1995): 79–86. http://dx.doi.org/10.1017/s0021859600071276.

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SUMMARYHerbage intake estimation, using the n-alkane technique, requires the determination of the ratio of ‘dosed’ to ‘natural’ n-alkane concentrations in faeces. The present study was designed to evaluate the accuracy and precision of intake estimates based on the analysis of pooled samples of total daily faecal output and those derived from rectal grab samples.Wether lambs maintained in individual metabolism crates, offered fresh herbage ad libitumand dosed daily with 120 mg of n-dotriacontane (C32), were used. Actual intake was recorded daily and samples of total faecal output were retained

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2

Mellen, Samantha, Maria de Ferrars, Claire Chapman, Sarah Bevan, James Turvill, and Daniel Turnock. "Evaluation of sample stability for a quantitative faecal immunochemical test and comparison of two sample collection approaches." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 55, no. 6 (2018): 657–64. http://dx.doi.org/10.1177/0004563218766393.

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Background Faecal immunochemical testing is increasingly being used to triage symptomatic patients for suspected colorectal cancer. However, there are limited data on the effect of preanalytical factors on faecal haemoglobin when measured by faecal immunochemical testing. The aim of this work was to evaluate the stability of faecal haemoglobin in faeces and to compare two methods of faecal haemoglobin sampling for faecal immunochemical testing. Methods Six patients provided faeces for faecal haemoglobin measurement which were transferred into specialized collection devices at baseline and at 1

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3

Rehbein, S., T. Lindner, M. Visser, and R. Winter. "Evaluation of a double centrifugation technique for the detection of Anoplocephala eggs in horse faeces." Journal of Helminthology 85, no. 4 (2010): 409–14. http://dx.doi.org/10.1017/s0022149x10000751.

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AbstractFaecal samples of 250 horses from farms with a known history of tapeworm infection were examined comparatively for cestode eggs using a double centrifugation/combined sedimentation–floatation technique. From each faecal sample, three 5 g and three 15 g subsamples were processed, each using either saturated NaCl solution, specific gravity (sp. g.) 1.2 [NaCl]; concentrated sugar solution, sp. g. 1.26 [sugar]; or concentrated ZnSO4 solution, sp. g. 1.3 [ZnSO4] for floatation. In total, faeces from 187 horses ( = 74.8%) tested ‘positive’ for Anoplocephala eggs. Percentages of samples testi

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4

Mioto da Costa, M. C., L. C. V. Ítavo, C. C. B. Ferreira Ítavo, et al. "Evaluation of internal and external markers to estimate faecal output and feed intake in sheep fed fresh forage." Animal Production Science 59, no. 4 (2019): 741. http://dx.doi.org/10.1071/an16567.

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The objectives of the present study were to estimate dry-matter intake and output of faeces using two external and four internal markers in sheep fed Brachiaria brizantha cv. Marandu on an ad libitum basis over 3- and 5-day periods. Six sheep fitted with ruminal cannulas were randomly assigned in a crossover design experiment to receive either of two treatments of external markers, namely titanium dioxide (TiO2) and chromic oxide (Cr2O3). Faecal output was obtained by total faecal collection and faecal grab sampling. Faeces were collected for 3- or 5-day periods, and, for each collection perio

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5

Piggott, Maxine P. "Effect of sample age and season of collection on the reliability of microsatellite genotyping of faecal DNA." Wildlife Research 31, no. 5 (2004): 485. http://dx.doi.org/10.1071/wr03096.

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Individual identification of animals from DNA in field-collected faecal samples is becoming an increasingly important tool in wildlife population monitoring. A major issue relevant to the application of this technique is the reliability of the genotypes obtained. I investigated the effect of sample age and season of collection on amplification rates and reliability of microsatellite genotypes amplified from faecal DNA of a marsupial herbivore, the brush-tailed rock-wallaby (Petrogale penicillata) and a eutherian carnivore, the red fox (Vulpes vulpes). Comparison of DNA profiles from 1 day to 6

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Luiselli, Luca, Valentín Pérez-Mellado, Mario Garrido, Ana Pérez-Cembranos, and Claudia Corti. "Using faecal samples in lizard dietary studies." Amphibia-Reptilia 32, no. 1 (2011): 1–7. http://dx.doi.org/10.1163/017353710x530212.

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AbstractWhile the use of faecal pellets is widely accepted as a primary methodological source of data for dietary studies, a recent paper advocated for the use of gut contents. This was due to the fact that faecal samples would give biased results of the diet of arthropod predators, due to a lower representation of soft-bodied prey in faecal pellets. To test this assumption, we compared the spring diet of several populations of two insular lizards from the Balearic Islands (Spain), Podarcis lilfordi and Podarcis pityusensis, using both faecal pellets and gut contents. Our results do not suppor

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Furnival, EP, JL Corbett, and MW Inskip. "Evaluation of controlled release devices for administration of chromium sesquioxide using fistulated grazing sheep. 1. Variation in marker concentration in faeces." Australian Journal of Agricultural Research 41, no. 5 (1990): 969. http://dx.doi.org/10.1071/ar9900969.

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A group of 10 sheep grazed as a single flock; five were dosed with chromium sesquioxide (Cr2O3) using an experimental controlled release device, and five were dosed twice a day with gelatin capsules. Total faecal output was collected from each sheep over a period of 13 days, and in addition, a sample was taken twice daily from the rectum (grab sample). In a second experiment total faeces were collected every 2 h for 24 h. Total faecal output was compared with estimates of faecal output calculated from the quantity of Cr2O3 in the dose, and the concentrations in representative samples and in gr

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8

Gray, Jessica, Nicole Masters, Aaron Wiegand, and Mohammad Katouli. "Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters." Canadian Journal of Microbiology 66, no. 11 (2020): 623–30. http://dx.doi.org/10.1139/cjm-2019-0499.

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We investigated the specificity and sensitivity of two horse-associated markers, HoF597 and Horse mtCytb, and 12 mitochondrial and bacterial markers of six animal species (human, cow, pig, bird, dog, chicken) in the faecal samples of 50 individual horses. Both horse markers were detected in 48 (96%) faecal samples. Cross-reactivity with dog (BacCan545) and pig (P23-2) occurred in 88% and 72% of horse faecal samples, respectively. Several other bacterial and mitochondrial markers of non-target hosts were also detected; however, their specificities were >80%. Analyses of samples from surface

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Pestell, Angela J. L., and Sophie Petit. "Diet of the western pygmy possum, Cercartetus concinnus Gould (Marsupialia: Burramyidae), at Innes National Park, South Australia, and evaluation of diet sampling methods." Australian Journal of Zoology 55, no. 5 (2007): 275. http://dx.doi.org/10.1071/zo07037.

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The diet of a population of western pygmy possums, Cercartetus concinnus Gould (Marsupialia: Burramyidae), at Innes National Park, South Australia, was examined using faecal and fur pollen swab samples in relation to monthly plant phenological data. Eucalyptus pollen was the most abundant in both faeces and in fur swab samples, followed by Melaleuca pollen; plant exudates could not be examined by this study. Moth scales were found in 26% of the scat samples. Faecal samples comprised most plant species identified (15 of 17), but up to 25% of plant species recorded from fur pollen swabs were not

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10

Tolleson, D. R., and J. P. Angerer. "The application of near infrared spectroscopy to predict faecal nitrogen and phosphorus in multiple ruminant herbivore species." Rangeland Journal 42, no. 6 (2020): 415. http://dx.doi.org/10.1071/rj20071.

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Near infrared spectroscopy (NIRS) was applied to determine faecal nitrogen and phosphorus using a temporo-spatially diverse dataset derived from multiple ruminant herbivore species (i.e. cattle, bison, deer, elk, goats, and sheep). Single-species NIRS calibrations have previously been developed to predict faecal constituents. Multi-species NIRS calibrations have previously been developed for herbivore faecal nitrogen but not for faecal phosphorus. Faecal samples representing a herd or flock composite were analysed via NIRS (400–2498nm). Calibration sets for faecal nitrogen and phosphorus were

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Bünger, Moritz, René Renzhammer, Anja Joachim, et al. "Trichurosis on a Conventional Swine Fattening Farm with Extensive Husbandry—A Case Report." Pathogens 11, no. 7 (2022): 775. http://dx.doi.org/10.3390/pathogens11070775.

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Helminth infections of swine regain clinical and economic importance due to the increasing demand for pork from extensive husbandry. Infections with Trichuris suis in pigs can lead to wasting and diarrhoea. This was demonstrated by a case of clinical trichurosis on a conventional fattening farm, where pigs were kept on pasture. While all pre-fattening pigs, which had not been on the pasture yet, had a good body condition and firm faeces, diarrhoea and poor body condition were observed in approximately half of the fattening pigs kept on pasture. Rectally collected faecal samples from all animal

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12

Devane, M., B. Robson, F. Nourozi, D. Wood, and B. J. Gilpin. "Distinguishing human and possum faeces using PCR markers." Journal of Water and Health 11, no. 3 (2013): 397–409. http://dx.doi.org/10.2166/wh.2013.122.

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Specificity testing of two published polymerase chain reaction (PCR) markers for the detection of human faecal pollution, revealed 100% false-positive rates to brush-tailed possum faeces (n = 10), but low false-positive rates against other potential pollution sources. Cross-reaction with possums could be a problem with other human-specific markers; therefore, a possum PCR marker was developed for use in conjunction with human PCR markers. The possum PCR marker was based on Bacteroidales 16S ribosomal ribonucleic acid sequences, and was tested on 233 individual faecal samples from 11 other anim

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13

Garg, Rajni, Prasanna Kumar Patil, Shoor Vir Singh, et al. "Comparative Evaluation of Different Test Combinations for Diagnosis ofMycobacterium aviumSubspeciesparatuberculosisInfecting Dairy Herds in India." BioMed Research International 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/983978.

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A total of 355 cows were sampled (serum,n=315; faeces,n=355; milk,n=209) from dairy farms located in the Punjab state of India. Faeces and serum/milk samples were screened by acid fast staining and “indigenous ELISA,” respectively. IS900PCR was used to screen faeces and milk samples. Bio-load of MAP in dairy cows was 36.9, 15.6, 16.3, and 14.4%, using microscopy, serum ELISA, milk ELISA and milk PCR, respectively. Estimated kappa values between different test combinations: serum and milk ELISA, faecal microscopy and faecal PCR, milk ELISA and milk PCR, faecal PCR and serum ELISA were 0.325, 0.

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14

Touyama, M., J. S. Jin, R. Kibe, H. Hayashi, and Y. Benno. "Quantification of Blautia wexlerae and Blautia luti in human faeces by real-time PCR using specific primers." Beneficial Microbes 6, no. 4 (2015): 583–90. http://dx.doi.org/10.3920/bm2014.0133.

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The Clostridium coccoides group, including the genus Blautia and other genera, is one of the predominant bacterial groups in the human intestine. We re-examined 266 human faecal clones and 58 isolates in the C. coccoides group isolated by Hayashi et al. (2002) in order to elucidate the detailed distribution of Blautia wexlerae and Blautia luti in human faeces. Subsequently, we designed a primer pair specific for B. wexlerae and B. luti based on the 16S ribosomal RNA (16S rRNA) gene sequence. The number of B. wexlerae and B. luti in faecal samples of 12 healthy Japanese subjects was examined by

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15

Hesta, M., S. Arnouts, and G. P. J. Janssens. "Dietary supplementation of coated butyrate in healthy dogs: effect on apparent digestibility, faecal flora and faecal volatile fatty acids." Veterinární Medicína 53, No. 3 (2008): 147–52. http://dx.doi.org/10.17221/1941-vetmed.

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The aim of the present study was to determine the effect of dietary addition of coated butyrate on gut health related faecal traits in dogs. A food with or without coated butyrate was tested in a crossover study with 17 dogs. Faecal samples were collected and frozen until analysis for proximate components. Fresh faecal samples were collected for bacterial culturing and short chain fatty acids (SCFA) analysis. The addition of coated butyrate to a dog diet had neither effect on faecal consistency score, dry matter content and faecal production nor on digestibility coefficients. Absolute and rela

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16

Inglis, G. D., L. D. Kalischuk, and H. W. Busz. "A survey ofCampylobacterspecies shed in faeces of beef cattle using polymerase chain reaction." Canadian Journal of Microbiology 49, no. 11 (2003): 655–61. http://dx.doi.org/10.1139/w03-087.

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A polymerase chain reaction (PCR)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. To ensure the removal of PCR inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control DNA (i.e., DNA designed to amplify with the Campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. In only two samples (0.5%) were the internal control or Campylobacter genus amplicons not detected. In the remaining 380 faecal samples, Campylobacter DNA was detected in 83%

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Taučer-Kapteijn, Maja, Wim Hoogenboezem, Remco Hoogenboezem, Sander de Haas, and Gertjan Medema. "Source tracking of Enterococcus moraviensis and E. haemoperoxidus." Journal of Water and Health 15, no. 1 (2016): 41–49. http://dx.doi.org/10.2166/wh.2016.209.

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Enterococci were detected occasionally in 100 L samples of water abstracted from a shallow aquifer in a natural dune infiltration area for drinking water production. Enterococcus moraviensis was the species most frequently identified in these samples. Because there are no existing reports of faecal sources of E. moraviensis and the closely related E. hemoperoxidus, this study aimed to find such sources of these two species in the dunes. Faecal samples from various animal species living in the vicinity of abstraction wells, were analysed for enterococci on Slanetz and Bartley Agar. From these s

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18

Suehiro, Yutaka, Yibo Zhang, Shinichi Hashimoto, et al. "Highly sensitive faecal DNA testing of TWIST1 methylation in combination with faecal immunochemical test for haemoglobin is a promising marker for detection of colorectal neoplasia." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 55, no. 1 (2017): 59–68. http://dx.doi.org/10.1177/0004563217691064.

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Background As TWIST1 methylation is specific to colorectal neoplasia, detection of TWIST1 methylation from faeces samples might be useful for colorectal neoplasia screening. However, because the content of human DNA in faeces is very small, it is very difficult to detect TWIST1 methylation by conventional bisulphite-based methylation assays. Therefore, we developed a new methylation assay without bisulphite treatment, the combined restriction digital PCR assay, and evaluated its sensitivity and specificity in combination with and without the faecal immunochemical test for haemoglobin for color

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Frosini, Siân-Marie, Georgina Gallow, Amanda Gibson, Juliana Menezes, Constança Pomba, and Anette Loeffler. "Detecting mecA in Faecal Samples: A Tool for Assessing Carriage of Meticillin-Resistant Staphylococci in Pets and Owners in the Microbiological ‘Fast Age’?" Microbiology Research 14, no. 1 (2023): 60–66. http://dx.doi.org/10.3390/microbiolres14010005.

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Sampling animals for carriage of meticillin-resistant, coagulase-positive staphylococci (MRCoPS), considered zoonotic pathogens, can be challenging and time-consuming. Developing methods to identify mecA from non-invasive samples, e.g., faeces, would benefit AMR surveillance and management of MRS carrier animals. This study aimed to distinguish MRS carriers from non-carriers from faecal samples using quantitative polymerase chain reaction (qPCR) for mecA. Paired faecal and nasal swab samples (n = 86) were obtained from 13 dogs and 20 humans as part of a longitudinal study. Nasal MRCoPS carriag

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Hama-Ali, Mohammed A., and Ayad H. Hasan. "Profiling of Bacterial Species from Covid-19 Faecal Samples in Kurdistan Region-Iraq." Science Journal of University of Zakho 11, no. 1 (2023): 11–15. http://dx.doi.org/10.25271/sjuoz.2023.11.1.1031.

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The invasion of intestinal cells by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may have an impact on the gut bacteria. This study investigated the alteration of gut bacteria during SARS-CoV-2 viral infection and after recovery. Faecal samples were collected from ten RT-PCR-conﬁrmed COVID-19 patients and five healthy participants (served as a control group) from November 21st, 2021 to April 1st, 2022. The faeces samples were collected three times, at the time of infection, after seven days of the infection and on day fifty after clearance of SARS-CoV-2. Serum samples were

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Tsuji, Yamato, Risma Yanti, Atsushi Takizawa, and Toshio Hagiwara. "Interspecific Difference in Seed Dispersal Characteristics between Japanese Macaques (Macaca fuscata) and Sympatric Japanese Martens (Martes melampus)." Folia Primatologica 91, no. 6 (2020): 711–20. http://dx.doi.org/10.1159/000509385.

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We compared the characteristics of seeds within faeces between semi-terrestrial Japanese macaques (<i>Macaca fuscata</i>) and sympatric arboreal Japanese martens (<i>Martes melampus</i>) in Shiga Heights, central Japan. We collected faecal samples of the two mammalian species for 1 year (<i>n</i> = 229 for macaques and <i>n</i> = 22 for martens). We then compared the proportion of seed occurrence, life-form composition, number of seeds and species richness within single faecal samples, and the seed intact ratio between the two mammalian species.

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RICHMOND, A. S., A. R. G. WYLIE, A. S. LAIDLAW, and F. O. LIVELY. "An evaluation of contrasting C32 alkane dosing and faecal sampling regimes to estimate herbage dry matter intake by beef cattle." Journal of Agricultural Science 153, no. 2 (2014): 353–60. http://dx.doi.org/10.1017/s0021859614000410.

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SUMMARYTwo experiments were carried out to evaluate different dosing and sampling regimes for estimating the dry matter intake (DMI) of vegetation by grazing beef cattle. Experiment 1 compared the DMI of a perennial ryegrass pasture by 48 grazing beef cattle dosed with C32n-alkane and faecally sampled at different time intervals. The cattle, of dairy and suckler origin, were balanced by origin and allocated to one of three alkane-dosing treatments (n=16): T1, dosed once daily (10.30 h) with two 500 mg boluses of C32 alkanes; T2, dosed twice daily (09.00 and 16.00 h) with a 500 mg bolus of C32n

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23

MIYASAKA, J., S. YAHIRO, Y. ARAHIRA, H. TOKUNAGA, K. KATSUKI, and Y. HARA-KUDO. "Isolation of Vibrio parahaemolyticus and Vibrio vulnificus from wild aquatic birds in Japan." Epidemiology and Infection 134, no. 4 (2005): 780–85. http://dx.doi.org/10.1017/s0950268805005674.

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Vibrio parahaemolyticus and Vibrio vulnificus were isolated from faecal samples of wild aquatic birds in winter. Although V. parahaemolyticus and V. vulnificus were present in low numbers in seawater in the area where the faecal samples of the birds were collected, the pathogens were isolated from the faeces of the birds. This study demonstrates that wild aquatic birds are a vehicle for V. parahaemolyticus and V. vulnificus to survive in winter.

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Allan, J. C., G. Avila, J. Garcia Noval, A. Flisser, and P. S. Craig. "Immunodiagnosis of taeniasis by coproantigen detection." Parasitology 101, no. 3 (1990): 473–77. http://dx.doi.org/10.1017/s0031182000060686.

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SUMMARYImmunodiagnostic tests for Taenia-specific faecal antigen based on polyclonal rabbit antisera against Taenia saginata or Taenia solium proglottid extracts in capture-type ELISA assays have been developed. Taenia-specific antigen was detected in detergent-solubilized faecal extracts from T. solium- and T. saginata-infected hosts. Coproantigen from T. solium-infected hamsters did not cross-react with faeces from rodents infected with Hymenolepis diminuta, H. citelli, H. micro-stoma, Necator americanus, Strongyloides ratti or Nematospiroides dubius and faeces from uninfected animals. When

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25

TUTENEL, A. V., D. PIERARD, J. URADZINSKI, et al. "Isolation and characterization of enterohaemorrhagic Escherichia coli O157[ratio ]H7 from cattle in Belgium and Poland." Epidemiology and Infection 129, no. 1 (2002): 41–47. http://dx.doi.org/10.1017/s0950268802007197.

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EHEC O157 were isolated from faeces of Belgian and Polish beef slaughter cattle. In Belgium, 1281 faecal samples were analysed by immunomagnetic separation [IMS] after enrichment in buffered peptone water from June 1998 till July 1999. Eighty-one samples (6.3%) were positive for E. coli O157. Phage type 8 was most frequently found. Bulls between 1 and 2 years old, slaughtered in September and October were most frequently found positive. Atypical biochemical features were observed in some isolates: 22 (27%) isolates were urease positive and 1 (1.2%) isolate was unable to ferment lactose. In Pol

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MES, T. H. M., H. W. PLOEGER, M. TERLOU, F. N. J. KOOYMAN, M. P. J. VAN DER PLOEG, and M. EYSKER. "A novel method for the isolation of gastro-intestinal nematode eggs that allows automated analysis of digital images of egg preparations and high throughput screening." Parasitology 123, no. 3 (2001): 309–14. http://dx.doi.org/10.1017/s0031182001008496.

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A simple and robust method for the isolation of gastro-intestinal nematode eggs from faeces is described that uses both salt- and sugar solutions for flotation. Application of this ‘salt–sugar’; isolation method to large numbers of faecal samples of adult dairy cows indicates a 3- or 4-fold reduction in the proportion of e.p.g.-negative cows relative to studies that used other techniques for egg isolation. The procedure detects more eggs than the Wisconsin flotation method in replicate samples and in spiked egg-free faeces. The number of recovered eggs in spiked faecal samples is linear over a

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Chezu, David Nnemakpanheme Samuel, Joan Gbonhinbor, and Patience Ndidi Amadi. "Prevalence of fascioliasis in cattle slaughtered at a municipal abattoir in Choba, Port Harcourt, Nigeria." Journal of Health, Applied Sciences and Management 7, no. 2 (2024): 220–26. http://dx.doi.org/10.4314/johasam.v7i2.22.

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The study was aimed at determining the prevalence and intensity of fascioliasis in cattle slaughtered at the abattoir in Choba Port Harcourt, Rivers State. A total of one hundred and forty-two (142) samples of cattle slaughtered at the abattoir were examined for fascioliasis, by direct microscopy of faeces and bile. Formol-ether concentration technique was used to examine the faecal and bile samples for the eggs of Fasciola gigantica. Out of the 142 samples examined, a total of 34 (23.9%) of faecal samples were positive for eggs of F. gigantic while a total of 90 (63.4%) of bile samples showed

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Norbury, GL. "A Comparison of Stomach and Fecal Samples for Diet Analysis of Grey Kangaroos." Wildlife Research 15, no. 3 (1988): 249. http://dx.doi.org/10.1071/wr9880249.

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In an experiment with four captive eastern grey kangaroos, Macropus giganteus, microscopic analysis of both stomach contents and faeces did not reflect the ingested dry weight proportion of most plant species in a fed diet. However, close agreement was obtained by correcting for unidentifiable microscopic fragments of plants. This suggests that differential digestion may not be as important an artefact of microscopic diet analysis as previously reported. Provided corrections are made, faecal analysis may be as reliable as stomach content analysis for describing relative proportions of plant sp

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Martinek, K., L. Kolářová, E. Hapl, I. Literák, and M. Uhrin. "Echinococcus multilocularis in European wolves (Canis lupus)." Parasitology research 87 (June 7, 2001): 838–39. https://doi.org/10.1007/s004360100452.

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Nested PCR was used as a diagnostic tool to investigate the presence of Echinococcus multilocularis tapeworm in protected species of carnivores in Slovakia. Twenty-three faecal samples from wolves, four samples from bears and three samples from lynx from the Muránska Planina mountains and eight samples from wolves from the Bukovské Vrchy mountains were examined. E. multilocularis DNA was detected in two samples of wolves' faeces from the Muránska Planina mountains and one sample from the Bukovské Vrchy mountains. Examination of bears' and lynx faecal samples gave negative results. The detectio

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DRIMTZIA, A., та E. PAPADOPOULOS. "Reduction rate of nematode egg counts and third-stage larvae development from sheep and goat faeces preserved at 4οC". Journal of the Hellenic Veterinary Medical Society 67, № 3 (2018): 177. http://dx.doi.org/10.12681/jhvms.15636.

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Gastrointestinal nematode parasites cause major production losses to small ruminants. The most common way to diagnose or monitor the worm burdens in sheep and goats remains the quantitative parasitological examinations, i.e. the faecal egg counts. However, the reliability of the results of such methods depends greatly on the conditions and duration of the storage of the faecal samples prior to examination. The aim of this research was to evaluate the reduction rate and the maximum storage period, without significant losses, of nematode egg counts and third-stage larvae development from sheep a

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Afonso, E., and A. C. Goydadin. "Molecular detection of Anaplasma phagocytophilum DNA in the lesser horseshoe bat (Rhinolophus hipposideros) guano." Epidemiology and Infection 146, no. 10 (2018): 1253–58. http://dx.doi.org/10.1017/s0950268818001279.

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AbstractAlthough bats are increasingly recognised as potential reservoir hosts of human zoonotic pathogens, bacteria in bats are still poorly studied. To investigate the DNA faecal prevalence of the bacterium Anaplasma phagocytophilum, we sampled 23 lesser horseshoe bat (Rhinolophus hipposideros) maternity colonies located in buildings (churches, barns) in rural villages of eastern France. A total of 552 faecal samples were collected from 278 individuals. Anaplasma phagocytophilum DNA was detected in the faeces of 63 individuals (22.7%). Such high prevalence might suggest persistent infection

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Afonso, E., and A.-C. Goydadin. "Molecular detection of Anaplasma phagocytophilum DNA in the lesser horseshoe bat (Rhinolophus hipposideros) guano." Epidemiology and Infection 146, no. 10 (2018): 1253–58. https://doi.org/10.5281/zenodo.13469476.

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(Uploaded by Plazi for the Bat Literature Project) Although bats are increasingly recognised as potential reservoir hosts of human zoonotic pathogens, bacteria in bats are still poorly studied. To investigate the DNA faecal prevalence of the bacterium Anaplasma phagocytophilum, we sampled 23 lesser horseshoe bat (Rhinolophus hipposideros) maternity colonies located in buildings (churches, barns) in rural villages of eastern France. A total of 552 faecal samples were collected from 278 individuals. Anaplasma phagocytophilum DNA was detected in the faeces of 63 individuals (22.7%). Such high pre

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Afonso, E., and A.-C. Goydadin. "Molecular detection of Anaplasma phagocytophilum DNA in the lesser horseshoe bat (Rhinolophus hipposideros) guano." Epidemiology and Infection 146, no. 10 (2018): 1253–58. https://doi.org/10.5281/zenodo.13469476.

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Abstract:

(Uploaded by Plazi for the Bat Literature Project) Although bats are increasingly recognised as potential reservoir hosts of human zoonotic pathogens, bacteria in bats are still poorly studied. To investigate the DNA faecal prevalence of the bacterium Anaplasma phagocytophilum, we sampled 23 lesser horseshoe bat (Rhinolophus hipposideros) maternity colonies located in buildings (churches, barns) in rural villages of eastern France. A total of 552 faecal samples were collected from 278 individuals. Anaplasma phagocytophilum DNA was detected in the faeces of 63 individuals (22.7%). Such high pre

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Afonso, E., and A.-C. Goydadin. "Molecular detection of Anaplasma phagocytophilum DNA in the lesser horseshoe bat (Rhinolophus hipposideros) guano." Epidemiology and Infection 146, no. 10 (2018): 1253–58. https://doi.org/10.5281/zenodo.13469476.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Although bats are increasingly recognised as potential reservoir hosts of human zoonotic pathogens, bacteria in bats are still poorly studied. To investigate the DNA faecal prevalence of the bacterium Anaplasma phagocytophilum, we sampled 23 lesser horseshoe bat (Rhinolophus hipposideros) maternity colonies located in buildings (churches, barns) in rural villages of eastern France. A total of 552 faecal samples were collected from 278 individuals. Anaplasma phagocytophilum DNA was detected in the faeces of 63 individuals (22.7%). Such high pre

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35

Afonso, E., and A.-C. Goydadin. "Molecular detection of Anaplasma phagocytophilum DNA in the lesser horseshoe bat (Rhinolophus hipposideros) guano." Epidemiology and Infection 146, no. 10 (2018): 1253–58. https://doi.org/10.5281/zenodo.13469476.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Although bats are increasingly recognised as potential reservoir hosts of human zoonotic pathogens, bacteria in bats are still poorly studied. To investigate the DNA faecal prevalence of the bacterium Anaplasma phagocytophilum, we sampled 23 lesser horseshoe bat (Rhinolophus hipposideros) maternity colonies located in buildings (churches, barns) in rural villages of eastern France. A total of 552 faecal samples were collected from 278 individuals. Anaplasma phagocytophilum DNA was detected in the faeces of 63 individuals (22.7%). Such high pre

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36

Afonso, E., and A.-C. Goydadin. "Molecular detection of Anaplasma phagocytophilum DNA in the lesser horseshoe bat (Rhinolophus hipposideros) guano." Epidemiology and Infection 146, no. 10 (2018): 1253–58. https://doi.org/10.5281/zenodo.13469476.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Although bats are increasingly recognised as potential reservoir hosts of human zoonotic pathogens, bacteria in bats are still poorly studied. To investigate the DNA faecal prevalence of the bacterium Anaplasma phagocytophilum, we sampled 23 lesser horseshoe bat (Rhinolophus hipposideros) maternity colonies located in buildings (churches, barns) in rural villages of eastern France. A total of 552 faecal samples were collected from 278 individuals. Anaplasma phagocytophilum DNA was detected in the faeces of 63 individuals (22.7%). Such high pre

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Gies, Anton, Tobias Niedermaier, Korbinian Weigl, Petra Schrotz-King, Michael Hoffmeister, and Hermann Brenner. "Effect of long-term frozen storage and thawing of stool samples on faecal haemoglobin concentration and diagnostic performance of faecal immunochemical tests." Clinical Chemistry and Laboratory Medicine (CCLM) 58, no. 3 (2020): 390–98. http://dx.doi.org/10.1515/cclm-2019-0878.

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AbstractBackgroundFaecal samples collected and stored frozen over years may be a valuable resource for efficient retrospective evaluation of faecal immunochemical tests (FITs). We aimed to assess how prolonged frozen storage and freeze-thaw cycles might affect measures of faecal haemoglobin (Hb) and diagnostic performance of FITs.MethodsFrom 2005 through 2010, participants of screening colonoscopy (n = 2042) and clinical colorectal cancer (CRC) cases (n = 184) provided faecal samples in stool containers (60 mL). The samples were stored at −80 °C for up to 11 years and underwent three freeze-th

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Grabow, W. O. K., T. E. Neubrech, C. S. Holtzhausen, and J. Jofre. "Bacteroides fragilis and Escherichia coli bacteriophages: excretion by humans and animals." Water Science and Technology 31, no. 5-6 (1995): 223–30. http://dx.doi.org/10.2166/wst.1995.0609.

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The faecal excretion of somatic and male-specific coliphages, and phages of Bacteroides fragilis strain HSP40, by humans and a variety of animals has been investigated as part of research on indicator features of phages. Ninety human stool specimens were obtained from black and white male and female individuals varying in age from 6 months to 85 years. Sixty-five faecal samples from domestic animals including cattle, sheep, pigs, horses, dogs, cats, geese and rabbits, were obtained from various agricultural and domestic sources. The National Zoological Gardens in Pretoria kindly supplied 38 st

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Stoops, M. A., L. Vollmer, and T. L. Roth. "167 FAECAL STEROID ANALYSES FOR MONITORING REPRODUCTIVE FUNCTION IN POLAR BEARS (URSUS MARITIMUS)." Reproduction, Fertility and Development 21, no. 1 (2009): 182. http://dx.doi.org/10.1071/rdv21n1ab167.

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Although there has been only limited research on polar bears (Ursus maritimus), it is known that they are seasonal breeders that mate in early spring and experience delayed implantation in the fall, giving birth to cubs in early winter. A non-invasive method for monitoring reproductive function in polar bears would facilitate the management of captive and wild polar bears while expanding the physiological database on this species. Longitudinal faecal steroid analyses were conducted on a captive female polar bear (8 years old) to identify suitable enzyme immunoassay (EIA) techniques for monitor

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Cooke, Andrew S., Kathryn A. Watt, Greg F. Albery, Eric R. Morgan, and Jennifer A. J. Dungait. "Lactoferrin quantification in cattle faeces by ELISA." PeerJ 8 (February 27, 2020): e8631. http://dx.doi.org/10.7717/peerj.8631.

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Background Promoting and maintaining health is critical to ruminant welfare and productivity. Within human medicine, faecal lactoferrin is quantified for routine assessment of various gastrointestinal illnesses avoiding the need for blood sampling. This approach might also be adapted and applied for non-invasive health assessments in animals. Methods In this proof-of-concept study, a bovine lactoferrin enzyme-linked immunosorbent assays (ELISA), designed for serum and milk, was applied to a faecal supernatant to assess its potential for quantifying lactoferrin in the faeces of cattle. Faecal l

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Verdier, Julien, Irene Raphaela Breunig, Margarete Clara Ohse, et al. "Faecal Micro-RNAs in Inflammatory Bowel Diseases." Journal of Crohn's and Colitis 14, no. 1 (2019): 110–17. http://dx.doi.org/10.1093/ecco-jcc/jjz120.

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Abstract Background and Aims Faecal biomarkers are used as indicators of disease activity in inflammatory bowel diseases [IBD], which include Crohn’s disease [CD] and ulcerative colitis [UC]. Micro-RNAs [miRNAs] are small non-coding RNAs detectable in extracellular fluids and can be used as clinical biomarkers. The aim of this study was to determine if faecal miRNA composition is altered in IBD. Methods More than 800 different human faecal miRNAs were measured in stool samples from control individuals and patients with active CD by using NanoString technology. Selected miRNAs were quantified b

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Tende, Talatuxs, Bengt Hansson, Ulf Ottosson, and Staffan Bensch. "Evaluating preservation medium for the storage of DNA in African lion Panthera leo faecal samples." Current Zoology 60, no. 3 (2014): 351–58. http://dx.doi.org/10.1093/czoolo/60.3.351.

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Abstract Lion faecal samples, collected in the field between 1 hour to 1 week after defecation were preserved in three different media (ethanol, ASL buffer and Two-step storage). The aim was to determine which faecal DNA field preservation method best enhances PCR amplification success. Samples stored in ethanol showed a significantly higher amplification success of microsatellite loci than samples stored in the other two media. In contrast, amplification success of a mitochondrial locus was similar among the samples stored in the three types of media. We reviewed twelve previous studies that

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Naser, Sabri M., Marc Vancanneyt, Evelyne De Graef, et al. "Enterococcus canintestini sp. nov., from faecal samples of healthy dogs." International Journal of Systematic and Evolutionary Microbiology 55, no. 5 (2005): 2177–82. http://dx.doi.org/10.1099/ijs.0.63752-0.

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The taxonomic position of strain LMG 13590T, originally isolated from dog faeces and classified as Enterococcus dispar in the BCCM/LMG Bacteria Catalogue, was reinvestigated. This strain and 12 recent isolates from faecal samples of healthy dogs occupied a clearly separate position when investigated with multilocus sequence analysis (MLSA) of the genes encoding the alpha subunit of ATP synthase (atpA), RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS). The 16S rRNA gene sequence of one representative strain showed highest similarities of 98–99 % with E. di

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Shah, Vikaskumar G., R. Hugh Dunstan, Phillip M. Geary, Peter Coombes, Timothy K. Roberts, and Ellak Von Nagy-Felsobuki. "Evaluating potential applications of faecal sterols in distinguishing sources of faecal contamination from mixed faecal samples." Water Research 41, no. 16 (2007): 3691–700. http://dx.doi.org/10.1016/j.watres.2007.04.006.

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Balestrieri, Alessandro, Luigi Remonti, and Claudio Prigioni. "Assessing carnivore diet by faecal samples and stomach contents: a case study with Alpine red foxes." Open Life Sciences 6, no. 2 (2011): 283–92. http://dx.doi.org/10.2478/s11535-010-0106-1.

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AbstractResearch on the feeding habits of mammalian carnivores relies mainly on the analysis of stomach contents and faecal samples, but the outcomes of these two methods have only been compared in a few studies, with contrasting conclusions. In an Alpine area of NW Italy, we analysed both fox faeces collected along standardised transects, and the stomach contents of road-killed individuals. Faecal analysis involved the identification of macroscopic fragments, the identification of earthworm chaetae, and the assessment of relative volumes using Kruuk and Parish’s technique. Use of both methods

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Van Gompel, Liese, Wietske Dohmen, Roosmarijn E. C. Luiken, et al. "Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers." Annals of Work Exposures and Health 64, no. 2 (2019): 125–37. http://dx.doi.org/10.1093/annweh/wxz098.

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Abstract Objectives Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. Methods During 2015–2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory

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Hernández-Dávila, O. A., J. Galindo-González, A. A. Castro-Luna, and V. J. Sosa. "Plastic sheets: a new method for collecting faecal samples with seeds dispersed by birds." Journal of Tropical Ecology 31, no. 2 (2014): 187–90. http://dx.doi.org/10.1017/s0266467414000716.

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Abstract:For birds, we tested the efficacy of a technique used to obtain faecal samples and their seed content from bats by placing plastic sheets below mist nets. This method was compared with that of collecting faecal samples using cotton bags. Plastic sheets were placed below each of eight mist nets to obtain faecal samples from birds caught in cloud forest remnants. Each bird was then placed separately in a cotton bag to catch any other seeds excreted. There were 84 faecal samples in total: 64 with no seeds and 20 with seeds; of the latter 65% were obtained from the plastic sheets. A total

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Bacsur, P., K. Szántó, T. Madácsy, et al. "P357 Importance of stool drug monitoring in anti-TNF-treated patients." Journal of Crohn's and Colitis 14, Supplement_1 (2020): S339. http://dx.doi.org/10.1093/ecco-jcc/jjz203.486.

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Abstract Background Anti-tumour necrosis factor (TNF) therapy has dramatically changed the treatment of inflammatory bowel disease (IBD). The relationship between clinical outcomes and serum anti-TNF levels is controversial. This study aimed to perform simultaneous analyses of the serum, mucosal and faecal infliximab and adalimumab levels to determine the mucosal expression of TNF-α and assess the relationship of the anti-TNF-α levels in these biological samples with the endoscopic and clinical activities and body composition in IBD patients. Methods Consecutive IBD patients who received maint

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Carapeto, Sandra, Eva Cunha, Isa Serrano, et al. "Effect of the Administration of a Lyophilised Faecal Capsules on the Intestinal Microbiome of Dogs: A Pilot Study." Genes 14, no. 9 (2023): 1676. http://dx.doi.org/10.3390/genes14091676.

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Faecal Microbiota Transplantation (FMT) is a promising strategy for modulating the gut microbiome. We aimed to assess the effect of the oral administration of capsules containing lyophilised faeces on dogs with diarrhoea for 2 months as well as evaluate their long-term influence on animals’ faecal consistency and intestinal microbiome. This pilot study included five dogs: two used as controls and three with diarrhoea. Animals were evaluated for four months by performing a monthly faecal samples collection and physical examination, which included faecal consistency determination using the Brist

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Yuan, Qixian, Shuai Zhu, Siqing Yue, et al. "Alterations in Faecal and Serum Metabolic Profiles in Patients with Neovascular Age-Related Macular Degeneration." Nutrients 15, no. 13 (2023): 2984. http://dx.doi.org/10.3390/nu15132984.

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Neovascular age-related macular degeneration (nAMD) is a common and multifactorial disease in the elderly that may lead to irreversible vision loss; yet the pathogenesis of AMD remains unclear. In this study, nontargeted metabolomics profiling using ultra-performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry was applied to discover the metabolic feature differences in both faeces and serum samples between Chinese nonobese subjects with and without nAMD. In faecal samples, a total of 18 metabolites were significantly altered in nAMD patients, and metabolic dysregu

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