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1
Hózer, Zoltán, Péter Windberg, Imre Nagy, et al. "Interaction of Failed Fuel Rods Under Air Ingress Conditions." Nuclear Technology 141, no. 3 (2003): 244–56. http://dx.doi.org/10.13182/nt03-a3365.
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ApSimon, H. T., A. Bartlett, R. Phadke, and M. S. Khangure. "Spontaneous Polymerisation of Cyanoacrylate/Lipiodol Mixtures Causing Failed Embolisation." Interventional Neuroradiology 2, no. 4 (1996): 255–61. http://dx.doi.org/10.1177/159101999600200402.
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In vitro experiments to investigate spontaneous polymerisation of n-butyl-2-cyanoacrylate when mixed with non ionic oily contrast medium are reported. The results suggest an interaction between the mixture components which is unique to one particular batch of Lipiodol Ultrafluid and Histoacryl n butyl cyanoacrylate. The interaction cannot be reproduced with other batches of Lipiodol Ultrafluid nor with an alternative preparation of n-butyl-2-cyanoacrylate. Implications for the use of the materials in neuro-interventional practice are discussed.
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Johnson, Chris. "Why human error modeling has failed to help systems development." Interacting with Computers 11, no. 5 (1999): 517–24. http://dx.doi.org/10.1016/s0953-5438(98)00041-1.
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Eichas, Kyle, Laura Ferrer-Wreder, Kari Trost, and Maria Rosaria Galanti. "Measuring Dimensions of Family Interaction in Adolescence." European Journal of Psychological Assessment 36, no. 5 (2020): 901–6. http://dx.doi.org/10.1027/1015-5759/a000555.
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Abstract. The aim of this study was to evaluate the convergent and discriminant validity of adolescent and parent ratings of family interaction. Adolescent and parent ratings of democratic parenting, parental warmth, and adolescent free disclosure were measured using a multitrait–multimethod confirmatory factor analytic approach. Participants included 3,959 Swedish youth in seventh grade (average age 13 years), with follow-up measurements in eighth and ninth grades. At each grade, findings provided support for trait discriminant validity and discriminant validity of methods. However, findings failed to provide support for convergent validity. Overall, the present findings suggest that measurement of family interactions should include the perspectives of both parents and adolescents because their perceptions of family interactions may represent different family interaction constructs.
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Jung, Y. H., S. J. Baik, and S. B. Ahn. "Investigation of Zircaloy-fuel interaction in failed spent PWR fuel using EPMA." Journal of Nuclear Materials 517 (April 2019): 349–55. http://dx.doi.org/10.1016/j.jnucmat.2019.02.025.
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Li, Songqing. "Book review: Nancy Bell, We Are Not Amused: Failed Humor in Interaction." Discourse Studies 18, no. 6 (2016): 771–73. http://dx.doi.org/10.1177/1461445616668086g.
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Stenström, Anna-Brita. "Review of Bell (2015): We are not amused. Failed humor in interaction." Functions of Language 23, no. 3 (2016): 384–90. http://dx.doi.org/10.1075/fol.23.3.05ste.
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Lidwell, K., J. Dillon, A. Sihota, V. O'Connor, and B. Pilkington. "Determining calmodulin binding to metabotropic glutamate receptors with distinct protein-interaction methods." Biochemical Society Transactions 32, no. 5 (2004): 868–70. http://dx.doi.org/10.1042/bst0320868.
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mGluRs (metabotropic glutamate receptors) are G-protein-coupled receptors that modulate synaptic transmission. The eight mammalian mGluRs form three groups based on sequence and functional similarities: group I (1 and 5), group II (2 and 3) and group III (4, 6–8) mGluRs. In the present study, we used a Y2H (yeast two hybrid) screen to identify proteins that interact with the C-terminal intracellular tail of mGluR3. Prominent among the candidate receptor interacting proteins was calmodulin, a Ca2+ sensor known to bind identifiable sequences in group I and III mGluRs. The Y2H method was used to investigate calmodulin binding to mGluRs but failed to confirm the documented interaction with group III mGluRs. Furthermore, subsequent biochemical analysis showed that calmodulin does not interact with group II mGluRs. This illustrates that certain Ca2+-dependent interactions are not recapitulated in yeast. Moreover, it highlights the necessity for supporting biochemical data to substantiate interactions identified with Y2H methods.
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Okada, Yusuke. "Repairing "Failed" Questions in Foreign Language Classrooms." JALT Journal 32, no. 1 (2010): 55. http://dx.doi.org/10.37546/jaltjj32.1-3.
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While the pedagogical value of teachers’ questioning in classrooms has been well studied, what exactly teachers do if a question fails to obtain an adequate response has not yet been sufficiently addressed from an interactional perspective. This study examines how and why foreign language teachers deal with this problem and how they pursue a response. Conversation analysis of EFL classroom interactions demonstrated that teachers employed three strategies to repair a question: a modification of the failed question in the target language, codeswitching into L1 as a further step of the modification, and proffering candidate responses to the failed question. Teachers do not merely simplify and sharpen the focus of the original question successively to pursue a response, but they teach English in interaction by trying to help students understand the meaning of the questions in English. Implications for teachers who face the problem of failed questions are discussed. これまで教師による「質問」の教育上の価値はよく研究されてきたものの、「質問」が学生の回答を得ることに失敗した場合に教師はどうするのかということは相互行為的視点からは十分に研究されてこなかった。本稿は外国語教室の中で教師が、失敗となった質問をどのようにそしてなぜその方法で扱い回答を求めていくのかを研究するものである。外国語としての英語教室での相互行為の会話分析により、教師は次の３つの方略を取ることが分かった。失敗となった質問の目標言語による改良、そしてその次の段階としての学生の第１言語へのコードスイッチイング、回答例の提案である。教師はターン毎に質問を易しくしたり焦点を絞ったりするだけでなく、目標言語である英語で「質問」を理解させようと試みることによって、英語を相互行為の中で教授していることが明らかとなった。最後に失敗となった質問という問題に直面する教師への示唆を議論し、本稿の結びとする。
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Holcomb, Valerie B., Hannes Vogel, and Paul Hasty. "Unlike p53, p27 failed to exhibit an anti-tumor genetic interaction with Ku80." Cell Cycle 8, no. 15 (2009): 2463–66. http://dx.doi.org/10.4161/cc.8.15.9249.
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Yook, Karen J., Stephen R. Proulx, and Erik M. Jorgensen. "Rules of Nonallelic Noncomplementation at the Synapse in Caenorhabditis elegans." Genetics 158, no. 1 (2001): 209–20. http://dx.doi.org/10.1093/genetics/158.1.209.
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Abstract Nonallelic noncomplementation occurs when recessive mutations in two different loci fail to complement one another, in other words, the double heterozygote exhibits a phenotype. We observed that mutations in the genes encoding the physically interacting synaptic proteins UNC-13 and syntaxin/UNC-64 failed to complement one another in the nematode Caenorhabditis elegans. Noncomplementation was not observed between null alleles of these genes and thus this genetic interaction does not occur with a simple decrease in dosage at the two loci. However, noncomplementation was observed if at least one gene encoded a partially functional gene product. Thus, this genetic interaction requires a poisonous gene product to sensitize the genetic background. Nonallelic noncomplementation was not limited to interacting proteins: Although the strongest effects were observed between loci encoding gene products that bind to one another, interactions were also observed between proteins that do not directly interact but are members of the same complex. We also observed noncomplementation between genes that function at distant points in the same pathway, implying that physical interactions are not required for nonallelic noncomplementation. Finally, we observed that mutations in genes that function in different processes such as neurotransmitter synthesis or synaptic development complement one another. Thus, this genetic interaction is specific for genes acting in the same pathway, that is, for genes acting in synaptic vesicle trafficking.
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Artymowicz, Pawel. "Growth and interaction of planets." Symposium - International Astronomical Union 202 (2004): 149–58. http://dx.doi.org/10.1017/s0074180900217737.
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We discuss theories of origin and evolution of the newly discovered extrasolar planetary systems. As these systems failed to fulfill prior expectations concerning their orbital structure, we are challenged to extend and/or revise many preexisting theories. Important extensions include migration of bodies in disks and planetary eccentricity pumping by planet-planet interaction and primordial disk-planet interaction. Progress in observational techniques will allow us to find which of these two types of interaction is responsible for the observed variety of orbits and masses of planets. New insights into the formation of giant planets in our system can be obtained by asking why Jupiter and Saturn are not larger, closer to the sun and/or do not follow noticeably elliptic orbits.
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Andres, Michael, Samuel Di Luca, and Mauro Pesenti. "Finger counting: The missing tool?" Behavioral and Brain Sciences 31, no. 6 (2008): 642–43. http://dx.doi.org/10.1017/s0140525x08005578.
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AbstractRips et al. claim that the principles underlying the structure of natural numbers cannot be inferred from interactions with the physical world. However, in their target article they failed to consider an important source of interaction: finger counting. Here, we show that finger counting satisfies all the conditions required for allowing the concept of numbers to emerge from sensorimotor experience through a bottom-up process.
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Yuan, Haiyue, Janko Ćalić, and Ahmet Kondoz. "Analysis of User Requirements in Interactive 3D Video Systems." Advances in Human-Computer Interaction 2012 (2012): 1–11. http://dx.doi.org/10.1155/2012/343197.
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The recent development of three dimensional (3D) display technologies has resulted in a proliferation of 3D video production and broadcasting, attracting a lot of research into capture, compression and delivery of stereoscopic content. However, the predominant design practice of interactions with 3D video content has failed to address its differences and possibilities in comparison to the existing 2D video interactions. This paper presents a study of user requirements related to interaction with the stereoscopic 3D video. The study suggests that the change of view, zoom in/out, dynamic video browsing, and textual information are the most relevant interactions with stereoscopic 3D video. In addition, we identified a strong demand for object selection that resulted in a follow-up study of user preferences in 3D selection using virtual-hand and ray-casting metaphors. These results indicate that interaction modality affects users’ decision of object selection in terms of chosen location in 3D, while user attitudes do not have significant impact. Furthermore, the ray-casting-based interaction modality using Wiimote can outperform the volume-based interaction modality using mouse and keyboard for object positioning accuracy.
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Al-Zahrani, Mona Yousef, and Abdullah Al-Bargi. "The Impact of Teacher Questioning on Creating Interaction in EFL: A Discourse Analysis." English Language Teaching 10, no. 6 (2017): 135. http://dx.doi.org/10.5539/elt.v10n6p135.
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This study examines the effect of questions on fostering interaction in English as a Foreign Language (EFL) classrooms. It also seeks to determine the characteristics of questions that promote increased classroom interaction. Data were collected through video recordings of EFL classrooms which were analyzed using Discourse Analysis techniques. Participants in the study are consisted of a group of intermediate-level English students at the English Language Institute (ELI) of a Saudi Arabian university. First, participating classes were video-recorded and the data gathered was transcribed. The questions asked in each class were then divided into two groups: questions that were deemed to promote classroom interaction and questions that failed to create classroom interaction. Finally, the defining features of each group of questions were determined. Results showed a correlation between the questions’ characteristics and the creation of classroom interaction. In other words, some question types significantly improved classroom interaction while others failed to do so.
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Josephs, Godwin C., and John O. Akerele. "Drug-drug interaction may explain failed antibiotic effectiveness - an in vivo study." Journal of Pharmacy & Bioresources 13, no. 2 (2016): 176. http://dx.doi.org/10.4314/jpb.v13i2.14.
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YANAGISAWA, Kazuaki, and Tore JOHNSEN. "In-Pile Eddy Current Test on PWR Fuel Rod Failed by Pellet-Cladding Interaction." Journal of Nuclear Science and Technology 23, no. 8 (1986): 752–55. http://dx.doi.org/10.1080/18811248.1986.9735049.
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Hiebert, Ted. "Hallucinating Ted Serios: the impossibility of failed performativity." Technoetic Arts 3, no. 3 (2005): 135–53. http://dx.doi.org/10.1386/tear.3.3.135/1.
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Hays, T. S., R. Deuring, B. Robertson, M. Prout, and M. T. Fuller. "Interacting proteins identified by genetic interactions: a missense mutation in alpha-tubulin fails to complement alleles of the testis-specific beta-tubulin gene of Drosophila melanogaster." Molecular and Cellular Biology 9, no. 3 (1989): 875–84. http://dx.doi.org/10.1128/mcb.9.3.875.
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In this paper we demonstrate that failure to complement between mutations at separate loci can be used to identify genes that encode interacting structural proteins. A mutation (nc33) identified because it failed to complement mutant alleles of the gene encoding the testis-specific beta 2-tubulin of Drosophila melanogaster (B2t) did not map to the B2t locus. We show that this second-site noncomplementing mutation is a missense mutation in alpha-tubulin that results in substitution of methionine in place of valine at amino acid 177. Because alpha- and beta-tubulin form a heterodimer, our results suggest that the genetic interaction, failure to complement, is based on the structural interaction between the protein products of the two genes. Although the nc33 mutation failed to complement a null allele of B2t (B2tn), a deletion of the alpha-tubulin gene to which nc33 mapped complemented B2tn. Thus, the failure to complement appears to require the presence of the altered alpha-tubulin encoded by the nc33 allele, which may act as a structural poison when incorporated into either the tubulin heterodimer or microtubules.
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Hays, T. S., R. Deuring, B. Robertson, M. Prout, and M. T. Fuller. "Interacting proteins identified by genetic interactions: a missense mutation in alpha-tubulin fails to complement alleles of the testis-specific beta-tubulin gene of Drosophila melanogaster." Molecular and Cellular Biology 9, no. 3 (1989): 875–84. http://dx.doi.org/10.1128/mcb.9.3.875-884.1989.
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In this paper we demonstrate that failure to complement between mutations at separate loci can be used to identify genes that encode interacting structural proteins. A mutation (nc33) identified because it failed to complement mutant alleles of the gene encoding the testis-specific beta 2-tubulin of Drosophila melanogaster (B2t) did not map to the B2t locus. We show that this second-site noncomplementing mutation is a missense mutation in alpha-tubulin that results in substitution of methionine in place of valine at amino acid 177. Because alpha- and beta-tubulin form a heterodimer, our results suggest that the genetic interaction, failure to complement, is based on the structural interaction between the protein products of the two genes. Although the nc33 mutation failed to complement a null allele of B2t (B2tn), a deletion of the alpha-tubulin gene to which nc33 mapped complemented B2tn. Thus, the failure to complement appears to require the presence of the altered alpha-tubulin encoded by the nc33 allele, which may act as a structural poison when incorporated into either the tubulin heterodimer or microtubules.
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Atapek, Ş. Hakan, Spiros G. Pantelakis, Apostolos N. Chamos, and Gülşah Aktaş Çelik. "Characterization of fatigue failed aged Cu-Ni-Si alloys." MATEC Web of Conferences 188 (2018): 02011. http://dx.doi.org/10.1051/matecconf/201818802011.
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The precipitation hardenable and non-toxic Cu-Ni-Si alloys are good alternatives to Cu-Be and Cu-Co-Ni-Be alloys due to their high strength and high conductivity that can be attained by not only alloying but also thermo-mechanical routes. In this study, the fractographic analysis was carried out to understand the fatigue failure of aged 2.55Ni-0.55Si-0.25Zr-0.25Cr (wt-%) alloy which is a member of Corson family. In fatigue tests, a constant amplitude loading was applied at a stress ratio (R = σmin/σmax) of -1 and different stress levels (400, 350, 200 and 175 MPa) were used. The fracture response of the alloy was discussed depending on the applied stress levels and microstructural features. It was concluded that (i) Ni,Zr-rich precipitates and Cr-rich precipitates at the grain boundaries caused crack nucleation at all stress levels and (ii) the interaction between Ni-rich silicides and dislocations at lower stress level resulted in localized shearing and fine striations.
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Lee, Byung Cheol, Jangwoon Park, Heejin Jeong, and Jaehyun Park. "Validation of Trade-Off in Human–Automation Interaction: An Empirical Study of Contrasting Office Automation Effects on Task Performance and Workload." Applied Sciences 10, no. 4 (2020): 1288. http://dx.doi.org/10.3390/app10041288.
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Automation aims to improve the task performance and the safety of human operators. The success of automation can be facilitated with well-designed human–automation interaction (HAI), which includes the consideration of a trade-off between the benefits of reliable automation and the cost of Failed automation. This study evaluated four different types of HAIs in order to validate the automation trade-off, and HAI types were configured by the levels and the statuses of office automation. The levels of automation were determined by information amount (i.e., Low and High), and the statues were decided by automation function (i.e., Routine and Failed). Task performance including task completion time and accuracy and subjective workload of participants were measured in the evaluation of the HAIs. Relatively better task performance (short task completion time and high accuracy) were presented in the High level in Routine automation, while no significant effects of automation level were reported in Failed automation. The subjective workload by the National Aeronautics and Space Administration (NASA) Task Load Index (TLX) showed higher workload in High and Failed automation than Low and Failed automation. The type of sub-functions and the task classification can be estimated as major causes of automation trade-off, and dissimilar results between empirical and subjective measures need to be considered in the design of effective HAI.
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Krause, Neal, and Estina Thompson. "Negative Interaction and Body Weight in Later Life." International Journal of Aging and Human Development 45, no. 4 (1997): 305–21. http://dx.doi.org/10.2190/qf0f-98g4-3jvk-nuxe.
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This study assesses the relationship between negative interaction and body mass index values among older adults. Throughout, an emphasis is placed on probing for individual differences in response to unpleasant encounters with significant others. Individual variations in personality (introversion-extraversion) as well as social status (gender) are evaluated within this context. Tests of the complex three-way interaction between negative interaction, gender, and introversion reveal that more negative interaction is associated with higher body mass index values among elderly women who are introverted. In contrast, a significant relationship between negative interaction, introversion, and body mass failed to emerge for older men.
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Pu, W., Q. Luo, L. Palaniyappan, et al. "Failed cooperative, but not competitive, interaction between large-scale brain networks impairs working memory in schizophrenia." Psychological Medicine 46, no. 6 (2016): 1211–24. http://dx.doi.org/10.1017/s0033291715002755.
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BackgroundA large-scale network named the default mode network (DMN) dynamically cooperates and competes with an external attention system (EAS) to facilitate various cognitive functioning that is prominently impaired in schizophrenia. However, it is unclear whether the cognitive deficit in schizophrenia is related to the disrupted competition and/or cooperation between these two networks.MethodA total of 35 schizophrenia patients and 30 healthy controls were scanned using gradient-echo echo-planar imaging during n-back working memory (WM) processing. Brain activities of the DMN and EAS were measured using general linear modelling of the functional magnetic resonance imaging data. Dynamic interaction between the DMN and EAS was decomposed into two directions using Granger causality analysis.ResultsWe observed a significant failure of DMN suppression in patients with schizophrenia, which was significantly related to WM/attentional deficit. Granger causality modelling showed that in healthy controls, while the EAS inhibitorily influenced the DMN, the DMN exerted an ‘excitatory’ or cooperative influence back on the EAS, especially in those with lower WM accuracy. In schizophrenia, this ‘excitatory’ DMN→EAS influence within the reciprocal EAS–DMN loop was significantly reduced, especially in patients with WM/attentional deficit.ConclusionsThe dynamic interaction between the DMN and EAS is likely to be comprised of both competitive and cooperative influences. In healthy controls, both the ‘inhibitory’ EAS→DMN interaction and ‘excitatory’ DMN→EAS interaction are correlated with WM performance. In schizophrenia, reduced ‘cooperative’ influence from the DMN to dorsal nodes of the EAS occurs in the context of non-suppression of the DMN and may form a possible pathophysiological substrate of WM deficit and attention disorder.
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Miranda, H. F., E. Silva, and G. Pinardi. "Synergy between the antinociceptive effects of morphine and NSAIDs." Canadian Journal of Physiology and Pharmacology 82, no. 5 (2004): 331–38. http://dx.doi.org/10.1139/y04-027.
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The intraperitoneal administration of morphine, diclofenac, ketoprofen, meloxicam, metamizol, paracetamol and piroxicam induced dose-dependent antinociception in mice tested with the acetic acid writhing test. The isobolographic analysis of the simultaneous intraperitoneal administration of fractions of the ED50's of morphine with each nonsteroidal anti-inflammatory drug (NSAID) demonstrated the existence of a supra-additive interaction (synergy). The selective antagonist of µ-opioid receptors naltrexone partially reversed the supra-additive interactions to additive interactions; however, the combinations of morphine/metamizol and morphine/paracetamol were completely antagonized, resulting in subadditive interactions. The selective antagonist of δ-opioid receptors naltrindole failed to significantly attenuate the combinations of morphine with ketoprofen, meloxicam and piroxicam, but decreased the activity of the combinations of morphine with diclofenac, metamizol and paracetamol, transforming the interactions from supra-additive to additive. Nor-binaltorphimine was used to evaluate the involvement of κ-opioid receptors. Nor-binaltor phimine did not modify the supra-additive interaction of morphine and NSAIDs and the additive interaction of the co-administration of morphine and metamizol. The synergy between morphine and NSAIDs could be related to different pathways of pain transmission, probably related to the different intracellular signal transduction mechanisms of action of opioid and non-opioid agents.Key words: writhing test, NSAIDs, morphine, antinociception, isobolographic analysis, synergy.
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Nakachi, Tatsuya, Shun Kohsaka, Masahisa Yamane, et al. "Scoring System for Identification of “Survival Advantage” after Successful Percutaneous Coronary Intervention in Patients with Chronic Total Occlusion." Journal of Clinical Medicine 9, no. 5 (2020): 1319. http://dx.doi.org/10.3390/jcm9051319.
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Background: Percutaneous coronary intervention (PCI) is widely used in patients with chronic total occlusion (CTO), but its benefit in improving long-term outcomes is controversial. We aimed to develop a prediction score for grading “survival advantage” conferred by successful results of CTO-PCI and a scoring system for prediction of the influence of CTO-PCI results on major adverse cardiac and cerebrovascular events (MACCEs). Methods: Follow-up data of 2625 patients who underwent CTO-PCI at 65 Japanese centers were analyzed. An integer scoring system was developed by including statistical effect modifiers on the association between successful CTO-PCI and one-year mortality. Results: Follow-up at 12 months was completed in 2034 patients. During follow-up, 76 deaths (3.7%) occurred. Patients with successful CTO-PCI had a better one-year survival than patients with failed CTO-PCI (log rank P = 0.016). Effect modifiers for the association between successful procedure and one-year mortality included diabetes (P interaction = 0.043), multivessel disease (P interaction = 0.175), Canadian Cardiovascular Society class ≥2 (P interaction = 0.088), and prior myocardial infarction (MI) (P interaction = 0.117). Each component was assigned a single point and summed to develop the scoring system. The patients were then categorized to specify the prediction of survival advantage by successful PCI: ≤2 (normal) and ≥3 (distinct). The differences in one-year mortality between patients with successful and failed treatment were −0.7% and 11.3% for normal and distinct score categories, respectively. In the scoring system for MACCE, score components were prior MI (P interaction = 0.19), left anterior descending artery (LAD)-CTO (P interaction = 0.079), and reattempt of CTO-PCI (P interaction = 0.18). The differences in one-year MACCEs between successful and failed patients for each score category (0, 1, and ≥2) were −1.7%, 7.5%, and 15.1%, respectively. Conclusions: The novel scoring system assessing the advantage of successful PCI can be easily applied in patients with CTO. It is a valid instrument for clinical decision-making while assessing the survival advantage of CTO-PCI and the influence of procedural results on MACCEs.
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Matsuyama, Sho, Yohko Kage, Noriko Fujimoto, et al. "Interaction between cardiac myosin-binding protein C and formin Fhod3." Proceedings of the National Academy of Sciences 115, no. 19 (2018): E4386—E4395. http://dx.doi.org/10.1073/pnas.1716498115.
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Mutations in cardiac myosin-binding protein C (cMyBP-C) are a major cause of familial hypertrophic cardiomyopathy. Although cMyBP-C has been considered to regulate the cardiac function via cross-bridge arrangement at the C-zone of the myosin-containing A-band, the mechanism by which cMyBP-C functions remains unclear. We identified formin Fhod3, an actin organizer essential for the formation and maintenance of cardiac sarcomeres, as a cMyBP-C–binding protein. The cardiac-specific N-terminal Ig-like domain of cMyBP-C directly interacts with the cardiac-specific N-terminal region of Fhod3. The interaction seems to direct the localization of Fhod3 to the C-zone, since a noncardiac Fhod3 variant lacking the cMyBP-C–binding region failed to localize to the C-zone. Conversely, the cardiac variant of Fhod3 failed to localize to the C-zone in the cMyBP-C–null mice, which display a phenotype of hypertrophic cardiomyopathy. The cardiomyopathic phenotype of cMyBP-C–null mice was further exacerbated by Fhod3 overexpression with a defect of sarcomere integrity, whereas that was partially ameliorated by a reduction in the Fhod3 protein levels, suggesting that Fhod3 has a deleterious effect on cardiac function under cMyBP-C–null conditions where Fhod3 is aberrantly mislocalized. Together, these findings suggest the possibility that Fhod3 contributes to the pathogenesis of cMyBP-C–related cardiomyopathy and that Fhod3 is critically involved in cMyBP-C–mediated regulation of cardiac function via direct interaction.
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Khu, Yee-Ling, Esther Koh, Siew Pheng Lim, et al. "Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase." Journal of Virology 75, no. 1 (2001): 205–14. http://dx.doi.org/10.1128/jvi.75.1.205-214.2001.
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ABSTRACT Interaction between viral proteins is necessary for viral replication and viral particle assembly. We used the yeast two-hybrid assay to identify interactions among all the mature proteins of the hepatitis C virus. The interaction between NS3 and NS3 was one of the strongest viral protein-protein interactions detected. The minimal region required for this interaction was mapped to a specific subdomain of 174 amino acids in the N terminus of the helicase region. Random mutations in the minimal region were generated by PCR, and mutants that failed to interact with a wild-type minimal fragment were isolated using the yeast two-hybrid assay as a screen. Three of these mutations resulted in a reduction or a loss of interaction between helicases. Analytical gel filtration showed that in the presence of an oligonucleotide, wild-type helicases form dimers whereas the mutants remain mostly monomeric. All three mutants were partially or almost inactive when assayed for helicase activity in vitro. Mixing a mutant helicase (Y267S) with wild-type helicase did not dramatically affect helicase activity. These data indicate that dimerization of the helicase is important for helicase activity. The mutations that reduce self-association of the helicase may define the key residues involved in NS3-NS3 dimerization.
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Bernat, Agnieszka, Paola Massimi, and Lawrence Banks. "Complementation of a p300/CBP defective-binding mutant of adenovirus E1a by human papillomavirus E6 proteins." Journal of General Virology 83, no. 4 (2002): 829–33. http://dx.doi.org/10.1099/0022-1317-83-4-829.
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Previous studies have shown that the human papillomavirus type 16 (HPV-16) E6 protein binds to p300/CBP and abrogates its transcriptional co-activator function. However, there is little information on the biological consequences of this interaction and discrepancy as to whether the interaction is high-risk E6 specific or not. We performed a series of studies to compare the interactions of HPV-18 and HPV-11 E6 with p300, and showed that both high- and low- risk E6 proteins bind p300. In addition, using a transformation-deficient mutant of adenovirus E1a, which cannot interact with p300, we demonstrated that HPV-16, HPV-18 and, to a lesser extent, HPV-11 E6, can complement this mutant in cell transformation assays. In contrast, a mutant of HPV-16 E6 which does not bind p300 failed to rescue the E1a mutant. These results suggest that the E6–p300 interaction may be important for the ability of HPV E6 to contribute towards cell transformation.
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He, Wei, Linwei Guo, Lei Wang, et al. "Host Genotype and Precipitation Influence of Fungal Endophyte Symbiosis and Mycotoxin Abundance in a Locoweed." International Journal of Molecular Sciences 20, no. 21 (2019): 5285. http://dx.doi.org/10.3390/ijms20215285.
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Many plant endophytes produce mycotoxins, but how host genetic variation influences endophyte colonization and mycotoxin production under natural conditions is poorly understood. This interaction has not been fully considered in many previous studies which used controlled experiments with agronomic or model plant species. Here, we investigated this interaction in a naturally occurring forb (a locoweed species) Oxytropis ochrocephala, its symbiotic endophyte Alternaria oxytropis, and the mycotoxin swainsonine. Host genetic variation was characterized by microsatellite markers. Endophyte infection rate and swainsonine levels were determined by PCR and HPLC, respectively. Genetic markers defined two distinct host populations and revealed that host genetics were significantly correlated with geographical location, elevation, and precipitation. As the host diverged, symbiotic interactions were reduced or failed to produce detectable swainsonine in one host population. Host genotype and precipitation had a significant impact in shaping swainsonine production at the population level. This study highlights the effect of host genotype in influencing this interaction in locoweeds.
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Leighton, Tiffany L., Neha Dayalani, Liliana M. Sampaleanu, P. Lynne Howell, and Lori L. Burrows. "Novel Role for PilNO in Type IV Pilus Retraction Revealed by Alignment Subcomplex Mutations." Journal of Bacteriology 197, no. 13 (2015): 2229–38. http://dx.doi.org/10.1128/jb.00220-15.
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ABSTRACTType IV pili (T4P) are dynamic protein filaments that mediate bacterial adhesion, biofilm formation, and twitching motility. The highly conserved PilMNOP proteins form an inner membrane alignment subcomplex required for function of the T4P system, though their exact roles are unclear. Three potential interaction interfaces for PilNO were identified: core-core, coiled coils (CC), and the transmembrane segments (TMSs). A high-confidence PilNO heterodimer model was used to select key residues for mutation, and the resulting effects on protein-protein interactions were examined both in a bacterial two-hybrid (BTH) system and in their nativePseudomonas aeruginosacontext. Mutations in the oppositely charged CC regions or the TMS disrupted PilNO heterodimer formation in the BTH assay, while up to six combined mutations in the core failed to disrupt the interaction. When the mutations were introduced into theP. aeruginosachromosome at thepilNorpilOlocus, specific changes at each of the three interfaces—including core mutations that failed to disrupt interactions in the BTH system—abrogated surface piliation and/or impaired twitching motility. Unexpectedly, specific CC mutants were hyperpiliated but nonmotile, a hallmark of pilus retraction defects. These data suggest that PilNO participate in both the extension and retraction of T4P. Our findings support a model of multiple, precise interaction interfaces between PilNO; emphasize the importance of studying protein function in a minimally perturbed context and stoichiometry; and highlight potential target sites for development of small-molecule inhibitors of the T4P system.IMPORTANCEPseudomonas aeruginosais an opportunistic pathogen that uses type IV pili (T4P) for host attachment. The T4P machinery is composed of four cell envelope-spanning subcomplexes. PilN and PilO heterodimers are part of the alignment subcomplex and essential for T4P function. Three potential PilNO interaction interfaces (the core-core, coiled-coil, and transmembrane segment interfaces) were probed using site-directed mutagenesis followed by functional assays in anEscherichia colitwo-hybrid system and inP. aeruginosa. Several mutations blocked T4P assembly and/or motility, including two that revealed a novel role for PilNO in pilus retraction, while other mutations affected extension dynamics. These critical PilNO interaction interfaces represent novel targets for small-molecule inhibitors with the potential to disrupt T4P function.
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Montes de Oca, Rocio, Paul R. Andreassen, Steven P. Margossian, et al. "Regulated interaction of the Fanconi anemia protein, FANCD2, with chromatin." Blood 105, no. 3 (2005): 1003–9. http://dx.doi.org/10.1182/blood-2003-11-3997.
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Abstract DNA damage activates the monoubiquitination of the Fanconi anemia (FA) protein, FANCD2, resulting in the assembly of FANCD2 nuclear foci. In the current study, we characterize structural features of FANCD2 required for this intranuclear translocation. We have previously identified 2 normal mRNA splice variants of FANCD2, one containing exon 44 sequence at the 3′ end (FANCD2-44) and one containing exon 43 sequence (FANCD2-43). The 2 predicted FANCD2 proteins differ in their carboxy terminal 24 amino acids. In stably transfected FANCD2—/— fibroblasts, FANCD2-44 and FANCD2-43 proteins were monoubiquitinated on K561. Only FANCD2-44 corrected the mitomycin C (MMC) sensitivity of the transfected cells. We find that monoubiquitinated FANCD2-44 was translocated from the soluble nuclear compartment into chromatin. A mutant form of FANCD2-44 (FANCD2-K561R) was not monoubiquitinated and failed to bind chromatin. A truncated FANCD2 protein (Exon44-T), lacking the carboxy terminal 24 amino acids encoded by exon 44 but retaining K561, and another mutant FANCD2 protein, with a single amino acid substitution at a conserved residue within the C-terminal 24 amino acids (D1428A), were monoubiquitinated. Both mutants were targeted to chromatin but failed to correct MMC sensitivity. Taken together, our results indicate that monoubiquitination of FANCD2 regulates chromatin binding and that D1428 within the carboxy terminal acidic sequence encoded by exon 44 is independently required for functional complementation of FA-D2 cells. We hypothesize that the carboxy terminus of FANCD2-44 plays a critical role in sensing or repairing DNA damage.
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Fard Jahromi, Soudabeh Sabetian, and Mohd Shahir Shamsir. "Construction and Analysis of the Cell Surface’s Protein Network for Human Sperm-Egg Interaction." ISRN Bioinformatics 2013 (August 12, 2013): 1–8. http://dx.doi.org/10.1155/2013/962760.
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Sperm-egg interaction is one of the most impressive processes in sexual reproduction, and understanding the molecular mechanism is crucial in solving problems in infertility and failed in vitro fertilization. The main purpose of this study is to map the sperm-egg interaction network between cell-surface proteins and perform an interaction analysis on this new network. We built the first protein interaction network of human sperm-egg binding and fusion proteins that consists of 84 protein nodes and 112 interactions. The gene ontology analysis identified a number of functional clusters that may be involved in the sperm-egg interaction. These include G-protein coupled receptor protein signaling pathway, cellular membrane fusion, and single fertilization. The PPI network showed a highly interconnected network and identified a set of candidate proteins: ADAM-ZP3, ZP3-CLGN, IZUMO1-CD9, and ADAM2-IZUMO1 that may have an important role in sperm-egg interaction. The result showed that the ADAM2 may mediate interaction between two essential factors CD9 and IZUMO1. The KEGG analysis showed 12 statistically significant pathways with 10 proteins associated with cancer, suggesting a common pathway between tumor fusion and sperm-egg fusion. We believe that the availability of this map will assist future researches in the fertilization mechanism and will also facilitate biological interpretation of sperm-egg interaction.
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Timco, G. W., and A. M. Cornett. "Laboratory tests of ice interaction with steel booms." Canadian Journal of Civil Engineering 23, no. 2 (1996): 560–66. http://dx.doi.org/10.1139/l96-060.
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A test program was carried out to study the interaction of ice with sections of ice-control booms. Two different boom shapes and four different boom sizes were tested. The results indicate that there can be several different failure modes for the ice during the interaction. Particularly high loads were obtained when the ice failed in a buckling failure mode. To minimize the ice-induced loads, the boom must be designed to prevent this failure mode and induce flexural failures in the ice. This note presents quantitative information on the loads measured for different boom shapes and sizes, and for different ice thickness. The results can be used to help optimize the design of a suitable boom for ice control. Key words: ice, ice control, ice booms, St. Lawrence River, loads, forces, design.
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Korgaonkar, Pradeep, and Bay O'Leary. "Product, Marketing and Web Site Attributes Discrimanating Between Successful and Failed Internet Businesses." Journal of Internet Commerce 7, no. 4 (2008): 485–512. http://dx.doi.org/10.1080/15332860802507347.
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Dayawansa, S., S. Peckins, S. Ruch, and R. Norgren. "Parabrachial and hypothalamic interaction in sodium appetite." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 300, no. 5 (2011): R1091—R1099. http://dx.doi.org/10.1152/ajpregu.00615.2010.
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Rats with bilateral lesions of the lateral hypothalamus (LH) fail to exhibit sodium appetite. Lesions of the parabrachial nuclei (PBN) also block salt appetite. The PBN projection to the LH is largely ipsilateral. If these deficits are functionally dependent, damaging the PBN on one side and the LH on the other should also block Na appetite. First, bilateral ibotenic acid lesions of the LH were needed because the electrolytic damage used previously destroyed both cells and axons. The ibotenic LH lesions produced substantial weight loss and eliminated Na appetite. Controls with ipsilateral PBN and LH lesions gained weight and displayed robust sodium appetite. The rats with asymmetric PBN-LH lesions also gained weight, but after sodium depletion consistently failed to increase intake of 0.5 M NaCl. These results dissociate loss of sodium appetite from the classic weight loss after LH damage and prove that Na appetite requires communication between neurons in the LH and the PBN.
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Fitzsimons, Kathleen, Ana Maria Acosta, Julius P. A. Dewald, and Todd D. Murphey. "Ergodicity reveals assistance and learning from physical human-robot interaction." Science Robotics 4, no. 29 (2019): eaav6079. http://dx.doi.org/10.1126/scirobotics.aav6079.
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This paper applies information theoretic principles to the investigation of physical human-robot interaction. Drawing from the study of human perception and neural encoding, information theoretic approaches offer a perspective that enables quantitatively interpreting the body as an information channel and bodily motion as an information-carrying signal. We show that ergodicity, which can be interpreted as the degree to which a trajectory encodes information about a task, correctly predicts changes due to reduction of a person’s existing deficit or the addition of algorithmic assistance. The measure also captures changes from training with robotic assistance. Other common measures for assessment failed to capture at least one of these effects. This information-based interpretation of motion can be applied broadly, in the evaluation and design of human-machine interactions, in learning by demonstration paradigms, or in human motion analysis.
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Nunez, Eleuda, Soichiro Matsuda, Masakazu Hirokawa, Junichi Yamamoto, and Kenji Suzuki. "Effect of Sensory Feedback on Turn-Taking Using Paired Devices for Children with ASD." Multimodal Technologies and Interaction 2, no. 4 (2018): 61. http://dx.doi.org/10.3390/mti2040061.
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Most children can naturally engage in play and by this, develop skills while interacting with their peers and toys. However, children with Autism Spectrum Disorder (ASD) often show impairments in play skills which result in limited opportunities for interaction with others and the learning of social skills. In this regard, robotic devices/toys that can provide simple and attractive indications are advantageous to engage children with ASD in play activities that require social and interaction skills. This project proposes a new interaction method using paired robotic devices called COLOLO to facilitate a fundamental exchange of intention in communication so-called turn-taking. These tangible devices are designed to sense the user’s manipulation, send a message to the paired device, and display visual cues for assisting children to achieve turn-taking through play. On the sessions with COLOLO there are two devices, one held by the therapist and one by the child, and they take turns to manipulate the toys and change their colors. In this article, two experimental conditions or interaction rules: the “two-sided lighting rule” and the “one-sided lighting rule" were introduced. The two interactions rules differ from each on the way the devices used the visual cues to indicate the turn-holder. The effect of each interaction rule on children’s turn-taking behaviors was investigated through an experimental study with four children with ASD. From the results, we found that with the one-sided lighting rule participants tended to shift their gaze more and to decrease the failed attempts of turn-taking. The discussion covers the possibilities of using paired devices to describe participants’ behaviors related to turn-taking quantitatively.
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Heath, Michèle C., Barbara Valent, Richard J. Howard, and Forrest G. Chumley. "Interactions of two strains of Magnaporthe grisea with rice, goosegrass, and weeping lovegrass." Canadian Journal of Botany 68, no. 8 (1990): 1627–37. http://dx.doi.org/10.1139/b90-209.
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Two strains of the fungus Magnaporthe grisea caused responses in goosegrass, weeping lovegrass, and rice that varied from no visible symptoms, through isolated discrete lesions with or without brown margins, to coalescing lesions that completely killed the inoculated areas of the leaf. The fungus sporulated under conducive conditions in all of the five plant–strain combinations that produced visible symptoms, but the degree of sporulation varied considerably. Microscopical examination revealed a previously unreported dimorphism in the infection hypha in all three plant species. In all combinations, some appressoria failed to form infection hyphae. In all but one combination, some infection hyphae failed to differentiate and (or) became restricted to a single epidermal cell with autofluorescent walls. Quantitative data showed that the presence and density of lesions reflected the frequency with which appressoria formed growing fungal colonies; lesion appearance depended on the interaction between such colonies and the leaf tissue. The results suggest that the different plant–strain combinations represent a continuum of plant–fungus interactions, and that small differences in the frequency of different plant–fungus interactions at the cellular level may have significant effects on macroscopic symptom development.
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Chang, Chiung-Ying, and Wei-Pang Huang. "Atg19 Mediates a Dual Interaction Cargo Sorting Mechanism in Selective Autophagy." Molecular Biology of the Cell 18, no. 3 (2007): 919–29. http://dx.doi.org/10.1091/mbc.e06-08-0683.
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Autophagy is a catabolic membrane-trafficking mechanism conserved in all eukaryotic cells. In addition to the nonselective transport of bulk cytosol, autophagy is responsible for efficient delivery of the vacuolar enzyme Ape1 precursor (prApe1) in the budding yeast Saccharomyces cerevisiae, suggesting the presence of a prApe1 sorting machinery. Sequential interactions between Atg19-Atg11 and Atg19-Atg8 pairs are thought responsible for targeting prApe1 to the vesicle formation site, the preautophagosomal structure (PAS), and loading it into transport vesicles, respectively. However, the different patterns of prApe1 transport defect seen in the atg11Δ and atg19Δ strains seem to be incompatible with this model. Here we report that prApe1 could not be targeted to the PAS and failed to be delivered into the vacuole in atg8Δ atg11Δ double knockout cells regardless of the nutrient conditions. We postulate that Atg19 mediates a dual interaction prApe1-sorting mechanism through independent, instead of sequential, interactions with Atg11 and Atg8. In addition, to efficiently deliver prApe1 to the vacuole, a proper interaction between Atg11 and Atg9 is indispensable. We speculate that Atg11 may elicit a cargo-loading signal and induce Atg9 shuttling to a specific PAS site, where Atg9 relays the signal and recruits other Atg proteins to induce vesicle formation.
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Dalton, Amanda K., Paul S. Murray, Diana Murray, and Volker M. Vogt. "Biochemical Characterization of Rous Sarcoma Virus MA Protein Interaction with Membranes." Journal of Virology 79, no. 10 (2005): 6227–38. http://dx.doi.org/10.1128/jvi.79.10.6227-6238.2005.
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ABSTRACT The MA domain of retroviral Gag proteins mediates association with the host cell membrane during assembly. The biochemical nature of this interaction is not well understood. We have used an in vitro flotation assay to directly measure Rous sarcoma virus (RSV) MA-membrane interaction in the absence of host cell factors. The association of purified MA and MA-containing proteins with liposomes of defined composition was electrostatic in nature and depended upon the presence of a biologically relevant concentration of negatively charged lipids. A mutant MA protein known to be unable to promote Gag membrane association and budding in vivo failed to bind to liposomes. These results were supported by computational modeling. The intrinsic affinity of RSV MA for negatively charged membranes appears insufficient to promote efficient plasma membrane binding during assembly. However, an artificially dimerized form of MA bound to liposomes by at least an order of magnitude more tightly than monomeric MA. This result suggests that the clustering of MA domains, via Gag-Gag interactions during virus assembly, drives membrane association in vivo.
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Worthen, G. S., L. A. Smedly, M. G. Tonnesen, et al. "Effects of shear stress on adhesive interaction between neutrophils and cultured endothelial cells." Journal of Applied Physiology 63, no. 5 (1987): 2031–41. http://dx.doi.org/10.1152/jappl.1987.63.5.2031.
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The effect of hydrodynamic shear stress on the adhesive interaction between neutrophils and endothelial cells in vitro was investigated using an apparatus similar to a cone-in-plate viscometer. Isolated bovine neutrophils were labeled with 111In and incubated with monolayers of cultured bovine pulmonary arterial endothelial cells in the presence of different degrees of shear stress. Physiologically relevant shear (less than 2 dyn/cm2) was associated with marked decrease in neutrophil adherence. Stimulation with 10% bovine zymosan-activated plasma increased adherence under static conditions but failed to increase adherence conducted during the application of shear stress. Inhibition of endothelial cell prostacyclin production by meclofenamate or aspirin failed to alter the response to shear. Incubation of neutrophils under static conditions for 10, but not 5, min however, markedly enhanced subsequent resistance to shear, suggesting that a time-dependent reaction between neutrophil and endothelial cell was required to induce an increase in the strength of adherence. Analysis of neutrophil migration underneath the monolayer indicated that such migration in no way accounted for resistance to shear, particularly since shear resistance was enhanced on serum-coated plastic as well as endothelial cells. We conclude that hemodynamic factors may play an important role in modulating neutrophil adherence to endothelium in both normal and inflammatory states.
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Mir, Riyaz A., Aditya Bele, Sameer Mirza, et al. "A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression." Molecular and Cellular Biology 36, no. 6 (2015): 886–99. http://dx.doi.org/10.1128/mcb.00594-15.
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Ecdysoneless (ECD) is an evolutionarily conserved protein whose germ line deletion is embryonic lethal. Deletion ofEcdin cells causes cell cycle arrest, which is rescued by exogenousECD, demonstrating a requirement ofECDfor normal mammalian cell cycle progression. However, the exact mechanism by which ECD regulates cell cycle is unknown. Here, we demonstrate that ECD protein levels and subcellular localization are invariant during cell cycle progression, suggesting a potential role of posttranslational modifications or protein-protein interactions. Since phosphorylated ECD was recently shown to interact with the PIH1D1 adaptor component of the R2TP cochaperone complex, we examined the requirement of ECD phosphorylation in cell cycle progression. Notably, phosphorylation-deficient ECD mutants that failed to bind to PIH1D1in vitrofully retained the ability to interact with the R2TP complex and yet exhibited a reduced ability to rescueEcd-deficient cells from cell cycle arrest. Biochemical analyses demonstrated an additional phosphorylation-independent interaction of ECD with the RUVBL1 component of the R2TP complex, and this interaction is essential for ECD's cell cycle progression function. These studies demonstrate that interaction of ECD with RUVBL1, and its CK2-mediated phosphorylation, independent of its interaction with PIH1D1, are important for its cell cycle regulatory function.
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Liu, Bindong, Renke Dai, Chun-Juan Tian, Liza Dawson, Robert Gorelick, and Xiao-Fang Yu. "Interaction of the Human Immunodeficiency Virus Type 1 Nucleocapsid with Actin." Journal of Virology 73, no. 4 (1999): 2901–8. http://dx.doi.org/10.1128/jvi.73.4.2901-2908.1999.
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ABSTRACT The nucleocapsid (NC) domain of the retrovirus Gag protein plays several important roles in the viral life cycle, including virus assembly, viral genomic RNA encapsidation, primer tRNA placement, and enhancement of viral reverse transcription. In this study, deletion of NC domain of human immunodeficiency virus type 1 (HIV-1) Gag was found to drastically reduce virus particle production in CD4+ T cells. Cellular fractionation experiments showed that although most of the uncleaved wild-type HIV-1 Gag, unmyristylated Gag, and p6Gag domain-truncated Gag molecules copurified with the host cell cytoskeleton, most of the mutant Gag molecules lacking both the NC and p6Gag domains failed to cofractionate with cytoskeleton. In wild-type virus-infected cells, in which the viral protease was active, the cleaved NCp7 copurified with the cytoskeleton, whereas most of the MAp17 and CAp24 did not. Monoclonal antibody against actin coimmunoprecipitated full-length Gag and p6Gag domain-truncated Gag molecules from cell lysates but failed to precipitate the truncated mutant Gag molecules lacking NC plus p6Gag. Purified recombinant NCp7, but not CAp24, was able to bind F-actin in cosedimentation experiments. Furthermore, wild-type NCp7 and a zinc finger mutant NCp7(F16A), like a cellular actin-binding protein (the villin headpiece), bound F-actin in a dose-dependent fashion in vitro. Taken together, these results suggest that HIV-1 NCp7 can bind F-actin directly and that interaction between HIV-1 Gag and the actin cytoskeleton through the NC domain may play an important role in HIV-1 assembly and/or other steps of the viral life cycle.
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Mora Cortez, Roberto, and Wesley J. Johnston. "How to recover B2B relationships after a failed online reverse auction." Journal of Business & Industrial Marketing 35, no. 3 (2019): 551–63. http://dx.doi.org/10.1108/jbim-02-2019-0095.
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Purpose This paper aims to explore the possible scenarios after a failed reverse auction to continue a current buyer–seller relationship. Design/methodology/approach The authors developed a further understanding of reverse auctions through the examination of a longitudinal case study in the mining industry based on grounded theory. Findings The study indicates that losing a reverse auction is not a death sentence for the current supplier. Four factors influence the potential scenarios: buyer factors, supplier factors, buyer–seller factors and contextual factors. If the overall evaluation favors the current buyer–seller relationship, the supplier can continue the business interaction by full renegotiation or discrete step-by-step reconsideration. Conversely, the buyer–seller relationship would reach a state of dissolution. Originality/value This manuscript contributes to the understanding of reverse auction, an under-researched theme in organizational buying behavior theory. This paper is the first attempt to link buyer–seller relationship dissolution and reverse auctions. The authors suggest that more academic endeavors are needed to study online reverse auctions.
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Mehrotra, Ravi, Sujay Datta, and Deshdeep Sahdev. "Vortex–Vortex Interaction Mediated Motion of Vortices in Josephson Junction Arrays." Modern Physics Letters B 11, no. 09n10 (1997): 421–29. http://dx.doi.org/10.1142/s0217984997000529.
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We numerically study vortex motion in arrays of over- and under-damped Josephson junctions. We show quantitatively that vortex–vortex interactions (VVI) in a vortex-street are large enough to overcome the pinning potential and cause vortices to move even in a region with no external current. The experiments of van der Zant et al.1 are reinterpreted in terms of VVI without invoking ballistic motion of vortices. The discrepancy with simulations which have all failed to observe any ballistic motion is thus removed. Our results also indicate that the vortex mass is negligible for the McCumber–Stewart parameter, β c < 100.
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Banas, John. "Bell, Nancy (2015). We are not amused: Failed humor in interaction. Berlin, Germany/Boston: De Gruyter Mouton." EuroAmerican Journal of Applied Linguistics and Languages 3, no. 2 (2016): 60–62. http://dx.doi.org/10.21283/2376905x.5.71.
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Yang, Ming, Yixing Wang, Lei Guo, and Chun-Ming Liu. "Complex haploinsufficient interaction between APC11 and CYCLIN A1;2/TARDY ASYNCHRONOUS MEIOSIS in embryo development and seed germination in Arabidopsis." F1000Research 10 (January 28, 2021): 53. http://dx.doi.org/10.12688/f1000research.47692.1.
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Background:Complex haploinsufficiency is characterized by individuals with two heterozygous loci producing a phenotype that is not seen in either of the corresponding single-locus heterozygous individuals. The mutants of the anaphase-promoting complex/cyclosome (APC/C) subunit geneAPC11and the mitotic cyclin geneCYCLIN A1;2/TARDY ASYNCHRONOUS MEIOSIS(TAM) inArabidopsis thalianaare embryo-lethal and defective in meiosis, respectively, but their heterozygous single mutants do not exhibit defective embryo development and seed germination.Methods:Crosses between two heterozygousapc11mutant alleles and two homozygoustammutant alleles, and between two heterozygousapc11mutant alleles and aTAM:TAM-GFPline were conducted. Phenotypes of the F1seeds were analyzed by light microscopy.Results:We found that F1embryos from the crosses between heterozygousapc11-1(APC11/apc11-1) and homozygoustam-2(tam-2/tam-2) or betweenAPC11/apc11-2andtam-2/tam-2were morphologically normal but all the seeds failed to germinate. F1embryos from the crosses betweenAPC11/apc11-2andtam-1/tam-1(weaker allele thantam-2) produced morphologically normal seeds that germinated to form mature plants. However, F1embryos from the crosses betweenAPC11/apc11-1andtam-1/tam-1were abnormal and the seeds failed to germinate. Moreover, F1embryos from the crosses betweenAPC11/apc11-1and aTAM:TAM-GFPline were arrested at early developmental stages while F1embryos from the crosses betweenAPC11/apc11-2and theTAM:TAM-GFPline appeared fully developed but the seeds failed to germinate.Conclusions:Our observations indicate that theapc11andtammutants have an allele-dependent complex haploinsufficient relationship in embryo development and seed germination.
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Hanson, Louise. "Two Dogmas of the Artistic-Ethical Interaction Debate." Canadian Journal of Philosophy 50, no. 2 (2019): 209–22. http://dx.doi.org/10.1017/can.2019.13.
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AbstractCan artworks be morally good or bad? Many philosophers have thought so. Does this moral goodness or badness bear on how good or bad a work is as art? This is very much a live debate. Autonomists argue that moral value is not relevant to artistic value; interactionists argue that it is. In this paper, I argue that the debate between interactionists and autonomists has been conducted unfairly: all parties to the debate have tacitly accepted a set of constraints which prejudices the issue against the interactionist. I identify two demands which are routinely placed on arguments seeking to establish interaction and argue that they are, in fact, mutually conflicting.There are two upshots. First, in light of this, it is unsurprising that arguments for interaction have failed to meet with everybody’s satisfaction. The constraints are such that no argument can meet them. Second, recognizing this helps us uncover a new, promising, but hitherto overlooked strategy for establishing artistic-ethical interaction.
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Li, Jie, Wei Du, Suzette Maynard, Paul R. Andreassen, and Qishen Pang. "Oxidative stress–specific interaction between FANCD2 and FOXO3a." Blood 115, no. 8 (2010): 1545–48. http://dx.doi.org/10.1182/blood-2009-07-234385.
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Abstract The molecular pathway by which Fanconi anemia (FA) proteins function in oxidative stress response has not been defined. Here we report the functional interaction of the FA protein Fanconi anemia complementation group D2 (FANCD2) and the forkhead transcription factor forkhead box O 3a (FOXO3a). FOXO3a colocalized with FANCD2 foci in response to oxidative stress. The FANCD2-FOXO3a complex was not detected in cells deficient for the FA core complex component FANCA but could be restored in corrected cells. Consistent with this, a nonmonoubiquitinated FANCD2 mutant failed to bind FOXO3a. Although both mitomycin C and ionizing radiation induced FANCD2 monoubiquitination, neither could induce the association of FANCD2 and FOXO3a. Overexpression of FOXO3a reduced abnormal accumulation of reactive oxygen species, enhanced cellular resistance to oxidative stress, and increased antioxidant gene expression in corrected but not mutant FA-D2 cells. The novel oxidative stress response pathway identified in this study, in which FANCD2 and FOXO3a converge, probably contributes to cellular antioxidant defense.