Relevant bibliographies by topics / Fatty acids (Analysis; Determination)

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Academic literature on the topic 'Fatty acids (Analysis; Determination)'

Author: Grafiati
Published: 4 June 2021
Last updated: 17 February 2022

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Journal articles on the topic "Fatty acids (Analysis; Determination)"

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Gabriel, Hancu, Tero-Vescan Amelia, Filip Cristina, and Rusu Aura. "Capillary Electrophoresis in the Analysis of Polyunsaturated Fatty Acids." Acta Medica Marisiensis 61, no. 4 (December 1, 2015): 378–81. http://dx.doi.org/10.1515/amma-2015-0103.

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AbstractThe aim of this study to inventory the main electrophoretic methods for identification and quantitative determination of fatty acids from different biological matrices. Critical analysis of electrophoretic methods reported in the literature show that the determination of polyunsaturated fatty acids can be made by: capillary zone electrophoresis, micellar electrokinetic chromatography and microemulsion electrokinetic chromatography using different detection systems such as ultraviolet diode array detection, laser induced fluorescence or mass – spectrometry. Capillary electrophoresis is a fast, low-cost technique used for polyunsaturated fatty acids analysis although their determination is mostly based on gas chromatography.
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Preet, Raman, and Raghbir Chand Gupta. "DETERMINATION OF FATTY ACIDS IN SOLANUM SURATTENSE BURM. F. BY USING GAS CHROMATOGRAPHY." Asian Journal of Pharmaceutical and Clinical Research 10, no. 8 (August 1, 2017): 60. http://dx.doi.org/10.22159/ajpcr.2017.v10i8.18314.

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Objective: This study aims to document the fatty acid composition of Solanum surattense Burm. f. collected from hot desert of India, Rajasthan.Methods: The fatty acid analysis was performed by gas chromatography-flame ionization detector (GC-FID). The operating conditions used to examine methyl esters of fatty acids are as follows. Fatty acids were converted into methyl esters (FAMEs) before GC analysis according to the standard methods by Ranganna (1986). Quantitative determinations of FAMEs were conducted using GC-FID and capillary column HP-88 Agilent Technologies.Results: The most abundant fatty was palmitic acid (13.2%), oleic acid (22.9%), and linoleic acid (11.9%). This plant is good source of important fatty acids including all the groups of saturated, monounsaturated, and polyunsaturated fatty acids (MUFAs and PUFAs) and can be used as a commercial source of fatty acids especially MUFAs and PUFAs.Conclusion: The plant is well studied for various pharmacological activities such as antiasthmatic, anticancer, cardiovascular, and hepatoprotective. Determination of fatty acid profiles in nutritional and clinical research with precision and fastness has become popular for human health and basic research.
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Pörschmann, S., J. Pörschmann, L. Liebetrau, I. Kühn, and R. Pätz. "Gas chromatographic analysis of free fatty acids. Part 2: Determination of free fatty acids during anaerobic putrefaction." Acta Biotechnologica 7, no. 5 (1987): 479–83. http://dx.doi.org/10.1002/abio.370070523.

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Savych, Alona, Svetlana Marchyshyn, and Roksolana Basaraba. "Determination of fatty acid composition content in the herbal antidiabetic collections." Pharmacia 67, no. 3 (September 14, 2020): 153–59. http://dx.doi.org/10.3897/pharmacia.67.e51812.

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It was determined the qualitative composition and the quantitative content of fatty acids in the herbal antidiabetic collection № 3, № 4, № 7, № 13, № 19, which are used in folk medicine for prevention and treatment of diabetes mellitus type 2 in Ukraine by GC/MS method. According to the results it was identified 8 fatty acids in the herbal antidiabetic collection № 3, 13 fatty acids in the herbal antidiabetic collection № 4, 12 fatty acids in the herbal antidiabetic collection № 7, 13 fatty acids in the herbal antidiabetic collection № 13 and 11 fatty acids in the herbal antidiabetic collection № 19. The saturated fatty acids were found to be dominated in the raw plant material. The results of the quantitative research showed that the herbal antidiabetic collection № 3 contains 16.13 mg/g of fatty acids, the herbal antidiabetic collection № 4 – 27.17 mg/g, the herbal antidiabetic collection № 7 – 31.98 mg/g, the herbal antidiabetic collection № 13 – 27.37 mg/g, the herbal antidiabetic collection № 19 – 18.79 mg/g. The GC/MS analysis of the fatty acid composition in the herbal antidiabetic collections has shown that this raw material has a lot of fatty acids, which can have a positive effect for the treatment and prevention of diabetes mellitus type 2.
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Müller, M., Š. Horníčková, P. Hrabě, and J. Mařík. "Analysis of physical, mechanical and chemical properties of seeds and kernels of Jatropha curcas  ." Research in Agricultural Engineering 61, No. 3 (June 2, 2016): 99–105. http://dx.doi.org/10.17221/10/2014-rae.

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The research was performed to examine the physical, mechanical and chemical properties of seeds and kernels of Jatropha curcas. The test parameters were the dimensions of the seeds and kernels, required energy for oil extraction, determination of fatty acids in the oil by gas chromatography method, determination of the iodine value, determination of the acid value, determination of total polyphenols by the Folin & Ciocault reagent and determination of tocopherols and tocotrienols (vitamin E) by High-performance liquid chromatography. It was ascertained that the size of the seed and kernel varies considerably. Pressing of whole seeds needs more energy (50%) than pressing of kernels. From a chemical point of view it seems to be very appropriate for a production of biofuels. Jatropha curcas contains more polyphenols and vitamin E, which act as antioxidants, than the rape. Due to the low content of unsaturated fatty acids it is chemically suitable to replace the rape-seed oil with Jatropha curcas oil.
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Belichovska, Daniela, Zehra Hajrulai-Musliu, Risto Uzunov, Katerina Belichovska, and Mila Arapcheska. "Fatty Acid Composition of Ostrich (Struthio Camelus) Abdominal Adipose Tissue." Macedonian Veterinary Review 38, no. 1 (March 1, 2015): 53–59. http://dx.doi.org/10.14432/j.macvetrev.2014.11.028.

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Abstract Fatty acid composition of foods has a great impact on nutrition and health. Therefore, thе determination and knowledge of the fatty acid composition of food is very important for nutrition. Due to the high nutritional characteristics of ostrich meat and its products, the research determining their quality is of topical interest. The aim of the present investigation was the determination of fatty acid composition of ostrich adipose tissue. The content of fatty acids was determined according to AOAC Official Methods of Analysis and determination was performed using a gas chromatograph with a flame-ionization detector (GC-FID). The results are expressed as a percentage of the total content of fatty acids. The method was validated and whereupon the following parameters were determined: linearity, precision, recovery, limit of detection and limit of quantification. The repeatability was within of 0.99 to 2.15%, reproducibility from 2.01 to 4.57%, while recovery ranged from 94.89 to 101.03%. According to these results, this method is accurate and precise and can be used for analysis of fatty acids in foods. It was concluded that the content of saturated fatty acids (SFA) accounted 34.75%, of monounsaturated fatty acids (MUFA) 38.37%, of polyunsaturated fatty acids (PUFA) 26.88%, of total unsaturated fatty acids (UFA) 65.25% and of desirable fatty acids (DFA) (total unsaturated + stearic acid) 70.37% of the analysed samples. The ratio polyunsaturated/saturated fatty acids accounted 0.77. The most present fatty acid is the oleic (C18:1n9c) with 28.31%, followed by palmitic (C16:0) with 27.12% and linoleic (C18:2n6c) acid with 25.08%. Other fatty acids are contained in significantly lower quantities.
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Nishiyama-Naruke, A., J. A. Souza, M. Carnelós, and R. Curi. "HPLC Determination of Underivatized Fatty Acids Saponified at 37°C Analysis of Fatty Acids in Oils and Tissues." Analytical Letters 31, no. 14 (November 1998): 2565–76. http://dx.doi.org/10.1080/00032719808005325.

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Taufik, Moh, Hanifah Nuryani Lioe, and Nancy Dewi Yuliana. "Evaluation of Major Fatty Acids Determination in Palm Oil by Gas Chromatography-Flame Ionization Detection." Jurnal Agritech 36, no. 03 (December 21, 2016): 308. http://dx.doi.org/10.22146/agritech.16603.

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The fatty acid composition of palm oil is the major factor influencing its physical and chemical properties. The purpose of this research was to evaluate the analytical performance of major fatty acids (palmitic acid, stearic acid, oleic acid, and linoleic acid) analysis in palm oil. Triglycerides of palm oil were derivatized to fatty acid methyl esters (FAMEs) by using boron trifluoride (BF3) in methanol. FAMEs were determined by gas chromatography-flame ionization detection (GC-FID) using DB-23 capillary column as stationary phase. The studied parameters were instrument performance analysis, the efficiency of fatty acid derivatization, stability of derivatized analytes, accuracy, repeatability, intra-lab reproducibility, ruggedness, and method uncertainty. The evaluation results showed the instrument linearity at a working range of 5 to 40 mg/mL marked by coefficient of determination (R2) between 0.991-0.995. Instrument limits of detection (LOD) and instrument limits of quantification (LOQ) for 4 major fatty acids analysis were 26-35 µg/mLand 86-128 µg/mL, respectively. The increase of fatty acid concentration led to the decrease of derivatization efficiency in the fatty acids analysis. The result also showed that derivatized analytes were stable during 24 h storage at freeze temperature. The average recovery values by spiking method with the spiking concentration at 50 and 90 mg/g sample were at 75-94 % for stearic and linoleic acids analysis, however those for palmitic and oleic acids analysis were considered very low (<40 %), due to their low derivatization efficiency. Repeatability and intra-lab reproducibility of 4 major fatty acids analysis were at acceptable ranges, 0.45-1.38 % and 1.15-2.03 %, respectively. Determination by varying the volume of derivatizing agent showed the rugged method. Uncertainty of repeatability (Ur) and uncertainty of reproducibility (Ur) were ranged at 1.84-9.02 mg/g and 1.40-10.65 mg/g, respectively. This method was considerably reliable for the analysis of less abundance fatty acids in palm oil, stearic and linoleic acids.
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Jensen, Nancy J., and Michael L. Gross. "Mass spectrometry methods for structural determination and analysis of fatty acids." Mass Spectrometry Reviews 6, no. 4 (1987): 497–536. http://dx.doi.org/10.1002/mas.1280060403.

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Zeile, W. L., D. A. Knauft, and C. B. Kelly. "A Rapid Non-Destructive Technique for Fatty Acid Determination in Individual Peanut Seed1." Peanut Science 20, no. 1 (January 1, 1993): 9–11. http://dx.doi.org/10.3146/i0095-3679-20-1-3.

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Abstract Modification of fatty acid composition can improve the keeping quality of peanut oil and may expand markets for peanut usage. Modification may involve hybridization to recombine existing genes, or the creation of new variability through mutagenesis or transformation with genes from other organisms. Identification of the fatty acid composition of individual seed could improve the chances of obtaining peanut genotypes with desired fatty acid composition. Published techniques for fatty acid analysis of individual peanut seed require the use of approximately half the cotyledonary seed tissue and utilize a process of solvent extraction and esterification. We have used a procedure that requires a small fraction of seed tissue and analyzes fatty acids through direct transmethylation. Comparisons were made between procedures using seven genotypes representing a wide range of fatty acid composition variability. Quantities of fatty acids with C&lt;20 were not statistically different between tests. While some differences were observed in longer-chained fatty acids (C≥20), the relative values among genotypes were similar. This procedure maintains greater integrity of the seed for planting purposes, requires less time and cost for fatty acid analysis, and can improve efficiency of individual seed analysis.
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Dissertations / Theses on the topic "Fatty acids (Analysis; Determination)"

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Jenske, Ramona. "Compound specific and enantioselective determination of 2- and 3-hydroxy fatty acids in food." Aachen Shaker, 2009. http://d-nb.info/996919910/04.

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Gao, Lei. "Determination of quantitative nutritional labeling compositional data of lipids by Nuclear Magnetic Resonance (NMR) spectroscopy." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111577.

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The application of Nuclear Magnetic Resonance (NMR) spectroscopy in the determination of nutrition labeling component data (NLCD) was investigated, with the intent of using this methodology as a primary method to calibrate FTIR instrumentation for NLCD confirmation or screening on a routine basis. Unlike previous NMR studies, this work used three strategies to attain accuracy and reproducibility of NLCD through: (i) appropriate setting of operational parameters for spectral acquisition; (ii) resonance selection by optimizing the signal in proportion to the nuclei population and (iii) integration of resonances by pre-defined fixed chemical shift ranges. Both of 13C NMR spectra and 1H NMR spectra were shown to provide robust and acceptable results on the condition of appropriate acquisition of spectra for quantization purposes and the adoption of standard procedures for spectral processing, integration and calculation purposes. A quantitative approach of NLCD including trans content was determined by the interpretation resonance signals of 13C's and 1H's from methylene groups presented in triglyceride complex of fats and oils. An alternative method based on partial-least-squares (PLS) calibrations was provided as well, the latter proved to be especially useful in dealing with overlapping bands frequently found in 1H spectra. With the diagnostic provided by PLS, the trans and cis signals were shown to be separated in 1H spectra. It is the premise for the trans fat determination based on 1H spectra. Unit conversion from mole to weight % was addressed and a solution was developed based on NMR data per se, without significant assumptions. Validation involving the analysis of three different lipid types (model triacylglycerols, refined and hydrogenated oils) demonstrated that NMR predictions of NLCD were in good agreement with those results either from samples' actual values as well as those obtained using GC and FTIR predictions. Thus with appropriate integration of instrumentation, software and spectral processing accessories, both 13C and 1H NMR can determine NLCD, but with the capability to determine trans, 1H NMR is more practical than 13C NMR due to its much shorter spectral acquisition time. Thus NMR can serve as a primary method for the calibration of FTIR instrumentation, a practical instrumental method for routine NLCD determination and screening.
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Al-Alawi, Ahmed Ali. "Novel approaches to automated quality control analyses of edible oils by Fourier transform infrared spectroscopy : determination of free fatty acid and moisture content." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100311.

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Three new quantitative Fourier transform infrared (FTIR) spectroscopic methods were developed for the analysis of edible oils: two procedures to measure free fatty acids (FFA) and one to measure moisture (H2O), the latter two methods ultimately being automated and implemented on an auto-sampler equipped FTIR spectrometer. The methods developed for FFA determination both convert FFAs to their carboxylate salts by means of acid/base reaction without causing oil saponification, one approach using 1-propanol, an oil-miscible solvent, and the other using methanol, an oil-immiscible solvent into which the FFA salts are extracted. The first method involves splitting oil samples into two halves, with one half treated with propanol containing base and the other half with propanol only. The spectra of each half is collected and a differential spectrum obtained, from which quantization is performed. The methanol procedure simply involves extracting FFA into methanol containing a weak base and quantitating the FFA salts produced. Both FFA methods determine the FFA content by measuring the v (COO-) absorbance at ∼1570 cm-1 relative to a reference wavelength of 1820 cm-1 from a differential spectrum relative to the solvent, the extraction procedure being superior in terms of both speed and sensitivity, being able to measure FFA levels down to ∼0.001%. The method developed for moisture determination involves extracting water in edible oils into dry acetonitrile and then quantitating it by measuring the absorbance of the OH stretching band (3629 cm-1) and/or the HOH bending band (1631 cm -1). All three methods were validated by standard addition experiments, evaluated for potential interferences, and, in the case of FFA determination, compared to the performance of AOCS official methods. The results indicated that the extraction-based procedures were superior to conventional wet chemical methods in both sensitivity and reproducibility. The FFA and H2O extraction procedures were subsequently automated by connecting an auto-sampler to the FTIR spectrometer and developing procedures and software algorithms to enable the analysis of up to 100 samples/h. The methods developed and implemented are a substantive improvement over conventional methods for the analysis of FFA and H2O in edible oils and provide a means by which QC and process laboratories can analyze large volumes of edible oils for these two important parameters.
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Oliveira, Claudimar de Jesus. "Avaliação comparativa, \"in vitro\" e \"in vivo\", das propriedades antioxidantes da dieta do estado de São Paulo com a Dieta Mediterrânea." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/9/9132/tde-22092017-110415/.

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O bom funcionamento do organismo está intimamente relacionado com o consumo alimentar, pois através dos alimentos obtemos os nutrientes essenciais para processos biológicos vitais. Várias pesquisas vêm sendo realizadas com a finalidade de se determinar a atividade antioxidante de compostos em alimentos e de forma geral, condutas e hábitos alimentares. Alguns padrões dietéticos são seguidos como modelo, sendo o caso da Dieta Mediterrânea. No presente estudo, avaliou-se a atividade antioxidante de rações alimentares (comumente denominadas dietas) consumidas em duas localidades: 1) estado de São Paulo e 2) Grécia. As rações foram elaboradas com alimentos coccionados e alimentos in natura, inerentes a cada população, obtidos através de relatórios de órgãos oficiais - Instituto Brasileiro de Geografia e Estatística (IBGE) e Eurapean Commission (Health Monitoring Programme - Data Food Networking - DAFNE). Após processamento, esses alimentos foram liofilizados e então, triturados. Para determinação da atividade antioxidante in vitro, alíquotas das rações foram desengorduradas, devido à natureza dos ensaios antioxidantes in vitro. Para determinação da atividade antioxidante in vitro foram realizados dois ensaios: cooxidação β-caroteno/ácido linoléico e 2,2-difenil-picrilhidrazil (DPPH). Além da atividade antioxidante in vitro, foram executados ensaios nas dietas para a determinação da composição centesimal, perfil de ácidos graxos e determinação dos compostos fenólicos totais. Após esse delineamento, realizou-se o ensaio biológico, onde foram utilizados 30 ratos machos, da linhagem Wistar, recém desmamados e submetidos a 30 dias de tratamento, sob condições ambientais controladas, após os quais, os animais foram sacrificados para a retirada de cérebro, fígado e plasma. Ensaios foram realizados nesses tecidos com a finalidade de se avaliar a eficácia da conduta dietoterápica: atividade de enzimas antioxidantes em tecidos alvo (fígado e cérebro), peroxidação de tecidos (fígado, cérebro e plasma) e perfil de ácidos graxos (fígado, cérebro e plasma). Os resultados obtidos indicaram que as duas dietas estudadas mantiveram a integridade orgânica. Um fato relevante é que a dieta do Estado de São Paulo foi composta por alimentos acessíveis e de hábito regular na população nacional estudada, sendo essa equiparada qualitativamente com a Dieta Mediterrânea, uma referência mundial. A Dieta Nacional do Estado de São Paulo (DNSP) possui atividade antioxidante similar à Dieta Mediterrânea (DM), utilizada como modelo de referência.
The good operation of the organism is intimately related with the alimentary consumption , since through the victuals we obtain essential nutrients for vital biological processes. Several researches have been accomplished with the purpose of being determined the antioxidant activity of composed in victuals and of general form, conducts and alimentary habits. Some dietary patterns are followed as model, being the case of the Mediterranean Diet. In the present study, the antioxidant activity of alimentary diets was evaluated (commonly denominated diets) consumed at two places: 1) state of São Paulo and 2) Greece. The diets were elaborated with victuals cooked and victuals in natura, inherent to each population, obtained through reports of official organs - Brazilian Institute of Geography and Statistics (IBGE) and European Commission (Health Monitoring Programme - it Dates Food Networking -DAFNE). After processing, those victuals were Iyophilized and then, triturated. For determination of the activity antioxidant in vitro, aliquots of the rations were degreased, due to the nature of the tests antioxidant in vitro. For determination of the activity antioxidant in vitro two rehearsals were accomplished: co-oxidation of linoléico acid and β-carotene and 2,2-difenil-picrilhidrazil (DPPH). Besides activity antioxidant in vitro, were made executed in the diets for the determination of the centesimal composition, profile of fat acids and determination of compositions total phenolics compounds. After that out line, took place the biological rehearsal, where 30 male mice were used, of the lineage Wistar, recently weaned and submitted to 30 days of treatment, under controlled environmental conditions, after the ones which, the animals were sacrificed for the brain retreat, liver and it shapes. Rehearsals were accomplished in those tissues with the purpose of evaluating the effectiveness of the diets: activity of antioxidant enzymes in woven objective (Iiver and brain), peroxidation of tissues (Iiver, brain and it shapes) and profile of fat acids (Iiver, brain and it shapes). The obtained results indicated that the two studied diets maintained the organic integrity. An important fact is that the diet of the State of São Paulo was composed by accessible victuals and of regular habit in the studied national population, being that comparison qualitative with the Mediterranean Diet, a world reference. The National Diet of the State of São Paulo (DNSP) it possesses similar antioxidant activity to a reference model, Mediterranean Diet (DM).
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Marais, Christiaan De Wet. "The determination of cis and trans fatty acid isomers in partially hodrogenated plant oils /." Link to online version, 2007. http://hdl.handle.net/10019/399.

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Heijden, Luuk van der. "Determination of the food sources and of the role of meiofauna in soft-bottom intertidal habitats of the Marennes-Oléron Bay, France, and the Sylt-Rømø Bight, Germany : importance of the microphytobenthos-meiofauna pathway, highlighted by community structure, trophic markers and linear inverse food web models." Thesis, La Rochelle, 2018. http://www.theses.fr/2018LAROS030/document.

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La méiofaune joue un rôle important dans le fonctionnement des habitats benthiques à substrat meuble (ex. flux de matière) en relation avec sa production élevée, sa position trophique intermédiaire et les importants transferts d’énergie vers les niveaux trophiques supérieurs qui y sont lié. Les relations trophiques et les flux de matière organique liés à la méiofaune restent néanmoins mal connus ou peu pris en compte. Afin de mieux appréhender le rôle de la méiofaune, la structure des communautés et les relations trophiques entre les sources de nourriture et ces consommateurs ont été déterminées dans cinq habitats à substrat meuble (i.e., vasière nue, herbier, zone à sables) de la baie de Marennes-Oléron, France, et de la baie de Sylt-Rømø, Allemagne, en tenant compte des variations temporelles. Le peuplement de méiofaune s’est trouvé être dominé par les nématodes et les copépodes benthiques. Les biomasses de microphytobenthos et de matière organique du sédiment sont apparues comme étant deux facteurs structurants pour les communautés. L’utilisation combinée de différents traceurs de la matière (i.e., isotopes stables, acides gras) a démontré que le microphytobenthos et les bactéries étaient les ressources trophiques majeures de la méiofaune dans les cinq habitats étudiés. Les mesures réalisées sur la structure des communautés et les données issues des traceurs de la matière ont été implémentées dans des modèles de réseaux trophiques. Dans tous les habitats, ces modèles ont mis en évidence que le flux de carbone dominant était issu du microphytobenthos, ceci démontrant les très faibles changements de comportements alimentaires malgré les importantes différences de sources trophiques en termes de disponibilité et de production des sources de nourriture entre ces différents habitats. Tous les groupes trophiques de nématodes, à l’exception des déposivores sélectifs, étaient particulièrement sélectifs et s’alimentaient majoritairement à partir de microphytobenthos, ceci étant à l’origine d’une forte production et d’un court temps de renouvellement de la méiofaune. En conclusion, cette thèse démontre le rôle important de la méiofaune dans les habitats à substrat meuble ainsi que l’importance de la relation trophique entre le microphytobenthos et la méiofaune dans le fonctionnement de ces réseaux trophiques
Meiofauna play an important role in ecosystem processes in soft-bottom benthic habitats, e.g. food web dynamics, related to their highproduction, their intermediate trophic position and the energy they transfer towards higher trophic levels. The trophic linkages and flows of organic matter related to the meiofauna remain poorly known or taken into account. To better assess the role of meiofauna, the community structure and trophic relationships between food sources and meiofauna were determined in five intertidal soft-bottom habitats (i.e., mudflat, seagrass bed, sandflat) of the Marennes-Oléron Bay, France, and the Sylt-Rømø Bight, Germany, taking temporal variations into account. Meiofauna communities were dominated by nematodes and benthic copepods. Biomass of microphytobenthos and of sediment organic matter were two of the major drivers of community structure. The combination of trophic markers (i.e., stable isotopes, fatty acids) demonstrated that microphytobenthos and bacteria were the major food sources of meiofauna in the five habitats. Information from community structure assessments and trophic marker analyses were implemented in food web models. In all habitats, these models demonstrated that the main flow of carbon to meiofauna originated from microphytobenthos, highlighting negligible changes in meiofauna feeding behavior besides the large differences in availability and productivity of food sources between these habitats. All trophic groups of nematodes, except for selective deposit feeding nematodes, were highly selective and mainly fed on microphytobenthos, resulting in a high production and a short turn-over time of meiofauna. In conclusion, this thesis demonstrated the important role of meiofauna in soft-bottom habitats as well as the importance of the trophic pathway from microphytobenthos to meiofauna in the functioning of these food webs
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Thambugala, Dinushika. "Analysis of genetic diversity and expression of genes involved in fatty acid composition in flax (Linum usitatissimum L.) and comparative genomic analysis of their loci." Theoretical and Applied Genetics, 2013. http://hdl.handle.net/1993/30665.

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Flax (Linum usitatissimum L.) is one of the richest plant sources of omega-3 fatty acids praised for their health benefits. In this study, the extent of the genetic variability for genes encoding stearoyl-ACP desaturase (SAD), fatty acid desaturase 2 (FAD2) and 3 (FAD3) was determined by sequencing the six paralogous genes from 120 flax accessions representing a broad range of germplasm including some EMS mutant lines. A total of 6 alleles for sad1 and sad2, 21 for fad2a, 5 for fad2b, 15 for fad3a and 18 for fad3b were identified. Deduced amino acid sequences of the alleles predicted 4, 2, 3, 4, 6, and 7 isoforms, respectively. Allele frequencies varied greatly across genes. Fad3a, with 110 SNPs and 19 indels, and fad3b, with 50 SNPs and 5 indels, showed the highest levels of genetic variation. While most of the SNPs and all the indels were silent mutations, both genes carried non-sense SNP mutations resulting in premature stop codons, a feature not observed in sad and fad2 genes. Some alleles and isoforms discovered in induced mutant lines were absent in the natural germplasm. Correlation of these genotypic data with fatty acid composition data of 120 flax accessions phenotyped in six field experiments revealed statistically significant correlations of some of the SAD and FAD isoforms on fatty acid composition, oil content and iodine value. The novel allelic variants and isoforms identified for the six desaturases will be a resource for the development of oilseed flax with unique and useful fatty acid profiles.
October 2015
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Jenske, Ramona [Verfasser]. "Compound-Specific and Enantioselective Determination of 2- and 3-Hydroxy Fatty Acids in Food / Ramona Jenske." Aachen : Shaker, 2009. http://d-nb.info/1159835012/34.

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Skinner, Joanna P. "Determination of Oxidized Lipids in Commonly Consumed Foods and Their Binding Affinity for PPARγ." Digital Archive @ GSU, 2012. http://digitalarchive.gsu.edu/nutrition_theses/32.

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Background: Foods rich in polyunsaturated fatty acids (PUFA) are susceptible to oxidation through heating or storage. Oxidized lipids are known to act as ligands for a transcription factor (PPAR-gamma) that affects adipocyte differentiation and insulin sensitivity. Objective: The purpose of this study was to determine the amounts of oxidation products of a variety of PUFA containing foods over time, and to determine whether extracted fats from these foods act as ligands for PPAR-gamma. Method: To study the effect of room-temperature storage on oxidation, 5 foods (walnuts, sunflower seeds, ground flax, fish oil capsules, and infant formula) were purchased and stored at room temperature for 1, 2, and 3 months. To determine oxidation levels in fried foods, French fries and chicken nuggets were used. Fat was extracted from each food and the levels of oxidation products were analyzed by spectrophotometry and kits designed to measure oxidation products. Using a fluorescence polarization-based ligand screening assay kit, fat extracted from foods was analyzed for its binding affinity for PPAR-gamma. Results: Among foods stored at room temperature, the levels of oxidation products did not change significantly with time. Most foods exhibited the highest levels of oxidation at the purchase date. Infant formula and ground flax demonstrated higher levels of oxidation products than did other foods. In preliminary ligand binding assays, extracted fat from French fries showed the greatest binding affinity for PPAR-gamma; a select few other oils showed slight affinity. Discussion: Surprisingly, storage time did not affect oxidation levels. The greatest amount of oxidation may occur during pre-purchase storage conditions. The processing of formula and ground flax may be the cause of the relatively higher oxidation levels in those foods. The binding affinity for PPAR-gamma demonstrated by French fries needs further investigation. Conclusion: Certain oxidized lipids from foods may act as ligands for PPAR-gamma. Further research is required not only to determine which component of these PUFA-containing foods activates PPAR-gamma but also to determine whether that component acts as an agonist or antagonist for PPAR-gamma.
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Bhagdeo, Mansi Pravin. "Determination of Extractables from Cranberry Seeds Using Supercritical CO₂." Thesis, Virginia Tech, 2004. http://hdl.handle.net/10919/9989.

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An alternative method for extraction of therapeutically beneficial compounds such as sterols, fatty acids, and tocopherols from cranberry seeds with pure SF CO2 has been provided. The supercritical fluid extraction (SFE) operating conditions such as extraction temperature, pressure of CO2, extraction time, and CO2 flow rate were optimized to maximize the extraction yield. The amount and type of SF extractables (pure CO₂) have been compared with Soxhlet extractables (hexane) to evaluate the feasibility of SFE as an alternative extraction method. The extractables obtained via hexane and SF CO₂, which were derivatized and identified by gas chromatography mass spectrometry (GC-MS), contained mostly methylated fatty acids.
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Books on the topic "Fatty acids (Analysis; Determination)"

1

Mossoba, Magdi M. Official methods for the determination of trans fats by gas chromatography and infrared methods. Urbana, IL: AOCS Press, 2008.

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Brown, David Jeremy. Fatty acid signature analysis in the milk of Antarctic fur seals and Southern elephant seals from South Georgia: Implications for diet determination. Birmingham: University of Birmingham, 2000.

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Alvarez, J. G. Practical manual on lipid analysis: A series of monographs. Mays Landing, NJ, USA: Norell Press, 1991.

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Briganti, Louis A. Fatty acid profiling and the identification of environmental bacteria for drinking water utilities. Denver, CO: AWWA Research Foundation and American Water Words Association, 1995.

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Coutteau, Peter. Intercalibration exercise on the qualitative and quantitative analysis of fatty acids in artemia and marine samples used in mariculture. Copenhagen, Denmark: International Council for the Exploration of the Sea, 1995.

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Cussion, Sylvia. Resin and fatty acids and adsorbable organic halides in reagent water and effluents: Report. [Rexdale, Ont.]: Quality Management Office, Ontario Ministry of the Environment, 1992.

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O'Neill, John P. Trans fatty acids: Occurence and measurement in foodstuffs and uptake in a selected Irish population group. Dublin: University College Dublin, 1998.

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Kamal-Eldin, Mohammed Salih Afaf. Seed oils of Sesamum indicum,L. and some wild relatives: A compositional study of the fatty acids, acyl lipids, sterols, tocopherols and lignans. Uppsala: Sveriges Lantbruksuniversitet, 1993.

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Institution, British Standards. British Standard Methods of analysis of fats and fatty oils ... .: Section 1.1 determination of litre weight in air. London: BSI, 1985.

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Mrozik, Agnieszka. Zmiany w składzie bakteryjnych kwasów tłuszczowych w czasie rozkładu fenolu w glebie. Katowice: Wydawn. Uniwersytetu Śląskiego, 2000.

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Book chapters on the topic "Fatty acids (Analysis; Determination)"

1

James, A. T. "Qualitative and Quantitative Determination of The Fatty Acids by Gas-Liquid Chromatography." In Methods of Biochemical Analysis, 1–59. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110249.ch1.

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Gómez-Cortés, Pilar, Manuela Juárez, and Miguel Angel de la Fuente. "Fatty Acids." In Handbook of Dairy Foods Analysis, 235–59. 2nd ed. Second edition. | Boca Raton : CRC Press, 2021.: CRC Press, 2021. http://dx.doi.org/10.1201/9780429342967-11.

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Caster, W. O. "A Critical Evaluation of the Gas Chromatographic Technique for Identification and Determination of Fatty Acid Esters, with Particular Reference to the Use of Analog and Digital Computer Methods." In Methods of Biochemical Analysis, 135–88. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110355.ch4.

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Holman, Ralph T. "Measurement of Polyunsaturated Fatty Acids." In Methods of Biochemical Analysis, 99–138. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110201.ch3.

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Munro, H. N., and A. Fleck. "The Determination of Nucleic Acids." In Methods of Biochemical Analysis, 113–76. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110324.ch5.

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Traitler, H., U. Richli, A. M. Kappeler, H. Winter, R. Munoz-Box, and N. Fournier. "Quantitative Determination of Prostanoids by Stable Isotope Dilution Gas Chromatography / Mass Spectrometry." In Dietary ω3 and ω6 Fatty Acids, 323–32. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-2043-3_29.

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Sjöuvall, Jan. "Separation and Determination of Bile Acids." In Methods of Biochemical Analysis, 97–141. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110300.ch3.

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Dobson, G. "Cyclic fatty acids: qualitative and quantitative analysis." In Lipid Analysis in Oils and Fats, 136–80. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4613-1131-7_5.

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Dabi-Lengyel, Emma, I. Zámbó, P. Tétényi, and Eva Héthelyi. "Analysis of Specific, Unsaturated Plant Fatty Acids." In Biological Role of Plant Lipids, 155–56. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-1303-8_38.

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Gunstone, F. D. "Fatty acids — Nomenclature, structure, isolation and structure determination, biosynthesis and chemical synthesis." In Fatty Acid and Lipid Chemistry, 1–34. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4615-4131-8_1.

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Conference papers on the topic "Fatty acids (Analysis; Determination)"

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Berezin, Kirill V., Konstantin N. Dvoretsky, Maria L. Chernavina, Anna V. Novoselova, Vladimir V. Nechaev, Ekaterina M. Antonova, Anatoly M. Lichter, Vladimir V. Smirnov, Oksana N. Grechukhina, and Maksim V. Kartashov. "The use of theoretical and experimental methods of Raman spectroscopy in the determination of unsaturated fatty acids in vegetable oils." In Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions, edited by Dmitry E. Postnov. SPIE, 2019. http://dx.doi.org/10.1117/12.2523319.

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Heiremans, J., M. Claeys, and A. G. Herman. "DETERMINATION OF CHOLESTERYL HYDROXYOCTADBCADIENOATES IN VASCULAR TISSUE BY HPLC AND ITS RELEVANCE TO ATHEROSCLEROSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643084.

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Accumulation of lipids in the intimal arterial layer, and of cholesterol esters in particular, has been recognised as an early and prominent phenomenon in atherogenesis. Several attempts have been made to link putative peroxidation of these lipids in vivo to causal or deteriorating etiological determinants of plaque formation. The occurrence in advanced human atheromata of oxidized derivatives of cholesteryl linoleate -a major polyunsaturated cholesterol ester species in plasma and vessel wall - has been described by Brooks et al. (Atherosclerosis, 1970,13,223) and a positive correlation between the amount of cholesteryl hydroxyoctadecadienoates (CHODES) and the stage of the lesion has also been reported. In addition Funk and Powell (J. Biol. Chem., 1985,260,7481) have found hydroxyoctadecadienoic acids in normal aorta of different species, wich were strikingly increased after alkaline hydrolysis of total lipids, and this in contrast with the arachidonic acid analogs. The aim of this study was to develop a sensitive and practical method for specific assay of CHODES, without resorting to laborious saponification and derivatisation procedures required for gas chromatographic analysis, which could moreover augment the risk for artefacts.Dog thoracal aorta was homogenised and lipids were extracted using the Folch method with CHCl3/CH30H;2/l containing 0.05mM butylated hydroxytoluene. Fractionation of CHODES from neutral lipids was carried out by thin-layer chromatography. For detection and quantification a high-performance liquid chromatography (HPI/2) assay method was developed, with UV monitoring at 232nm , a wavelength characteristic for conjugated dienes with vicinal hydroxyl function. Reference compounds and the internal standard for HPLC analysis were synthesized from linoleic acid and 10,13,16-docosatrienoic acid, respectively, by preparation of hydroxy fatty acids with soybean lipoxygenase and subsequent esterification to cholesterol esters with pancreas cholesterol esterase. Confirmation of the structural identity was obtained by mass spectrometry. Artefactual formation of CHODES ex vivo was investigated by subjecting radiolabeled cholesteryl linoleate through the analysis procedure. This method allows the specific detection of CHODES in non-atherosclerotic arteries which was hitherto only reported for human advanced atherosclerotic lesions and is proposed as a sensitive and specific probe for prospective survey of lipid peroxidation in atherosclerotic blood vessels.
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Vucane, Sanita, Martins Sabovics, Lauris Leitans, and Ingmars Cinkmanis. "Smartphone-based colorimetric determination of DPPH free radical scavenging activity in vegetable oils." In Research for Rural Development 2020. Latvia University of Life Sciences and Technologies, 2020. http://dx.doi.org/10.22616/rrd.26.2020.016.

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Free radicals can rapidly and irreversibly oxidize various structures, including unsaturated fatty acids in vegetable oils, which affect the sensory properties. Spectrophotometry is the most widely used method for the determination of free radical scavenging activity (RSA) using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Barrier to the further use of classical analytical methods to analyze biologically active compounds in foodstuffs is that equipment requires high cost and has limited mobility. One of solutions is to replace classical methods, such as spectroscopy, with smartphonebased colorimetry. Huawei P30 Lite smartphone was used for colorimetric detection. The free radical scavenging activity (RSA) in vegetable oil was detected using an application ‘Color Picker’, with image matching algorithm for red, green, and blue (RGB) model. RSA was expressed as percentage and measured by the DPPH method. The aim of the study was to determinate the total free radical scavenging activity with smartphone-based colorimetry. For the data comparison and accuracy spectrophotometer as analytical optical instrument was used. Eleven vegetable oils: sea buckthorn, sunflower, rice, macadamia nut, hemp, corn, grape, linseed, rapeseed, olive and milk thistle oils were selected for analysis. The best results with no significant differences (p>0.05) compared to smartphone-based colorimetry from spectrophotometry were determined using RG values. The poor results were detected by using B value (p<0.05) and were not suitable for determination of RSA. Smartphone-based colorimetry can be used in the determination of the RSA in vegetable oils.
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Abramova, E. P., and R. R. Ishberdina. "DETERMINATION OF THE AMOUNT OF PHOSPHORUS BY COLORIMETRIC METHOD IN FISH PRODUCTS." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS. DSTU-PRINT, 2020. http://dx.doi.org/10.23947/interagro.2020.1.326-328.

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S. Nimaiyar, M.R. Paulsen, and R. L. Nelson. "Rapid Analysis of Fatty Acids in Soybeans Using FTNIR." In 2004, Ottawa, Canada August 1 - 4, 2004. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2004. http://dx.doi.org/10.13031/2013.16976.

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Erdogan Eliuz, Elif Ayşe, Yusuf Sıcak, and Eyüp Başaran. "Determination of Minimum Inhibitory Concentration of Hypericum perforatum L. fatty acids against Three Pathogen." In 4th International Symposium on Innovative Approaches in Engineering and Natural Sciences. SETSCI, 2019. http://dx.doi.org/10.36287/setsci.4.6.137.

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Luo, Cheng, Suyi Cai, Linyan Jia, Xiang Tang, Ruinan Zhang, Gang Jia, Hua Li, Jiayong Tang, Guangmang Liu, and Caimei Wu. "Study on Accurate Determination of Volatile Fatty Acids in Rumen Fluid by Capillary Gas Chromatography." In 5th International Conference on Information Engineering for Mechanics and Materials. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/icimm-15.2015.73.

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Kuiper, Heather, Cynthia Campbell, and Hubert Vesper. "GC-MS Analysis of Very Long Chain Fatty Acids in Human Plasma." In Virtual 2021 AOCS Annual Meeting & Expo. American Oil Chemists’ Society (AOCS), 2021. http://dx.doi.org/10.21748/am21.22.

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Zygmunt, Bogdan, Anna Banel, Marta Wasielewska, and Monika Felchner-Zwirello. "Studies on development of headspace - gas chromatographic determination of volatile fatty acids in troublesome environmental samples." In 2010 International Conference on Chemistry and Chemical Engineering (ICCCE). IEEE, 2010. http://dx.doi.org/10.1109/iccceng.2010.5560436.

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Khotsena, Chatchawan, and Siraporn Potivichayanon. "Determination of Appropriate Conditions for Volatile Fatty Acids from Rubber Industrial Wastewater by GC-FID: Headspace Technique." In 2020 International Conference and Utility Exhibition on Energy, Environment and Climate Change (ICUE). IEEE, 2020. http://dx.doi.org/10.1109/icue49301.2020.9307095.

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Reports on the topic "Fatty acids (Analysis; Determination)"

1

Márquez-Ruiz, Gloria. Determination of Oxidized Monomeric, Dimeric and Oligomeric Triacylglycerols; Diacylglycerols and Free Fatty Acids. AOCS, September 2009. http://dx.doi.org/10.21748/lipidlibrary.39199.

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Shi, Xiaoyan, Simin Fan, Jia Yao, Yang Gao, and Qiu Chen. Efficacy and safety of omega-3 fatty acids on liver-related outcomes in patients with nonalcoholic fatty liver disease: a protocol for a systematic review and meta-analysis. International Platform of Registered Systematic Review and Meta-analysis Protocols, May 2020. http://dx.doi.org/10.37766/inplasy2020.5.0008.

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