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Journal articles on the topic 'FCS-free culture'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Looney, Ben, and ChangQing Xia. "Murine bone marrow-derived dendritic cells generated in serum free conditions display an immature phenotype and protect from T1D. (107.11)." Journal of Immunology 186, no. 1_Supplement (2011): 107.11. http://dx.doi.org/10.4049/jimmunol.186.supp.107.11.

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Abstract Dendritic cells possess the ability to modulate immune responses and therefore have been investigated as a cell based therapy to prevent or reverse Type 1 Diabetes. In the NOD mouse model, bone marrow-derived DCs (BM-DC) are generated from bone marrow precursors cultured with GM-CSF and IL-4 and also typically containing fetal calf serum (FCS). The effect of FCS on murine BM-DC has not been extensively investigated. In the present study we compare BM-DC generated in FCS free culture media (X-VIVO20) with BM-DC generated in culture media (RPMI1640) containing 10% FCS. Data show that CD

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2

Kočiková, Alena, Andrea Kolesarić, Frieder Koszik, Georg Stingl, and Adelheid Elbe-Bürger. "Murine Langerhans Cells Cultured Under Serum-Free Conditions Mature into Potent Stimulators of Primary Immune Responses In Vitro and In Vivo." Journal of Immunology 161, no. 8 (1998): 4033–41. http://dx.doi.org/10.4049/jimmunol.161.8.4033.

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Abstract The ability of Ag-pulsed dendritic cells (DC) to induce primary immune responses has led them to be used for vaccination purposes. However, irrelevant Ags (e.g., FCS) can also be taken up by DC during their isolation and culture and then presented in vivo. To circumvent this, we have established a serum-free (SF) culture system. Murine epidermal cell (EC) suspensions were prepared with and without FCS and cultured for 3 days either in SF or FCS-containing medium. In spite of the lower Langerhans cell (LC) yields under SF conditions, both SF- and FCS-cultured LC (SF-cLC, FCS-cLC) under

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3

Fujimori, Y., M. Ogawa, SC Clark, and GJ Dover. "Serum-free culture of enriched hematopoietic progenitors reflects physiologic levels of fetal hemoglobin biosynthesis." Blood 75, no. 8 (1990): 1718–22. http://dx.doi.org/10.1182/blood.v75.8.1718.1718.

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Abstract Adult erythroid progenitors produce significantly higher fetal hemoglobin (HbF) levels in cultures containing fetal calf serum (FCS) and erythropoietin (Ep) than in vivo. The precise mechanisms for this increased HbF production in culture have not been elucidated. We examined HbF biosynthesis by enriched human progenitors in serum-free (SF) culture. We measured globin chain biosynthesis by combination of isoelectric focusing and autoradiography and examined percent nucleated erythrocytes containing HbF (%FNRBC) using microscopic immunodiffusion. CD34 (My10)-positive marrow cells from

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Fujimori, Y., M. Ogawa, SC Clark, and GJ Dover. "Serum-free culture of enriched hematopoietic progenitors reflects physiologic levels of fetal hemoglobin biosynthesis." Blood 75, no. 8 (1990): 1718–22. http://dx.doi.org/10.1182/blood.v75.8.1718.bloodjournal7581718.

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Adult erythroid progenitors produce significantly higher fetal hemoglobin (HbF) levels in cultures containing fetal calf serum (FCS) and erythropoietin (Ep) than in vivo. The precise mechanisms for this increased HbF production in culture have not been elucidated. We examined HbF biosynthesis by enriched human progenitors in serum-free (SF) culture. We measured globin chain biosynthesis by combination of isoelectric focusing and autoradiography and examined percent nucleated erythrocytes containing HbF (%FNRBC) using microscopic immunodiffusion. CD34 (My10)-positive marrow cells from a normal

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5

Yeo, C. X., J. Rathjen, and D. K. Gardner. "112. FETAL CALF SERUM AFFECTS hESC METABOLISM AND GENE EXPRESSION LEADING TO DIFFERENTIATION IN CULTURE." Reproduction, Fertility and Development 22, no. 9 (2010): 30. http://dx.doi.org/10.1071/srb10abs112.

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Fetal calf serum (FCS) has conventionally been used to support the growth and maintenance of human embryonic stem cells (hESCs). FCS however, is an undefined complex mixture containing factors which potentially alter the functionality of hESCs. Inclusion of FCS during embryo culture negatively impacts embryo metabolism and viability but comparative studies on hESCs have been hindered by the lack of serum and feeder independent culture systems. Using a recently available defined culture system, the effects of FCS on hESC metabolism and pluripotentcy were investigated. Mel2 hESCs were grown at 3

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6

Daniaux, C., B. Verhaeghe, and I. Donnay. "133 INSULIN, TRANSFERRIN AND SELENIUM WITH OR WITHOUT BSA IN A SERUM-FREE CULTURE SYSTEM FOR BOVINE EMBRYO, AND ITS SUITABILITY FOR EMBRYOS CULTURED IN SMALL GROUPS." Reproduction, Fertility and Development 17, no. 2 (2005): 217. http://dx.doi.org/10.1071/rdv17n2ab133.

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Serum in embryo culture medium may be a potential cause of abnormal accumulation of lipid droplets, which is correlated to a higher sensitivity to cryopreservation. Moreover, serum may introduce pathogens. With the aim of developing a serum-free culture medium, we first (Experiment 1) investigated the effect of adding ITS (5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium) as a serum substitute in SOF medium on embryos cultured in large groups (20 embryos per culture drop of 20 μL) and we then (Experiment 2) analyzed the effect of adding BSA. In this second experiment, our serum-free cult

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7

Suzuki, Takao, Masatomo Takahashi, Tsukasa Miyagi, et al. "A Study on the Induction of Mesoderm Differentiation of Mouse Embryonic Stem Cell in Serum Free Culture." Blood 108, no. 11 (2006): 4161. http://dx.doi.org/10.1182/blood.v108.11.4161.4161.

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Abstract (Introduction) Several products which can maintain the growth of ES (Embryonic stem) cell with no feeder cells or fetal calf serum (FCS) is now commercially available. Culti Cell ®(CC) can be a substitute for FCS and has ability of mainitaining mouse ES cells without feeder cells. Previously, it was reported that ES cell differentiate into FLk (fetal liver kinase) positive cells derived from mesoderm in methylcellulose and FCS. We investigated whether ES cell is able to differentiate into Flk positive cell in CC and methylcellulose without FCS. (Materials and Methods) Thawed ES cells

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8

Wanner, Yvonne, Felix Umrath, Marc Waidmann, Siegmar Reinert, and Dorothea Alexander. "Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization." Stem Cells International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/8303959.

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Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we detected significantly higher population doubling times of PL-cultured JPCs in comparison to FCS-cultured JPCs. However, this result was not based on lower proliferation activities but on diminished cell si

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Sébire, G., D. Emilie, C. Wallon, et al. "In vitro production of IL-6, IL-1 beta, and tumor necrosis factor-alpha by human embryonic microglial and neural cells." Journal of Immunology 150, no. 4 (1993): 1517–23. http://dx.doi.org/10.4049/jimmunol.150.4.1517.

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Abstract The abilities of the different cells from human central nervous system (CNS) to produce IL-6, IL-1 beta, and TNF-alpha were tested in vitro using either cultures enriched in human embryonic microglial cells or primary cultures of human embryonic CNS cells. High amounts of IL-6, low amounts of IL-1 beta but no TNF-alpha were detected in supernatants of microglial cells, kept either in FCS-free conditions or in FCS-containing medium. Moreover, IL-6 mRNA was also present in 45 to 55% of microglial cells cultured in the presence of FCS as visualized by in situ hybridization, whereas IL-1

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Pöhland, R., S. Lenz, J. Vanselow, and W. Tomek. "Detection of gene expression and enzyme activity of Cytochrom P450 arom (aromatase) in preantral and early antral bovine follicles depending on culture conditions in vitro." Archives Animal Breeding 49, no. 1 (2006): 29–40. http://dx.doi.org/10.5194/aab-49-29-2006.

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Abstract. This study reveals that cultivation of preantral and early antral (< 500 μm) follicles in culture medium containing FCS results in an expression of cytochrome P450 arom. (aromatase). The enzymatic activity of aromatase, measured in terms of the estradiol synthesis, was proved to be present in follicles greater than 100 μm, could further be stimulated by FSH in follicles greater than 300 μm diameter. The enzyme protein and the gene expression were studied by means of western blot and immunohistochemistry as well as by means of realtime PCR. Neither the protein nor the corresponding

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11

Danalache, Marina, Sophie-Maria Kliesch, Marita Munz, Andreas Naros, Siegmar Reinert, and Dorothea Alexander. "Quality Analysis of Minerals Formed by Jaw Periosteal Cells under Different Culture Conditions." International Journal of Molecular Sciences 20, no. 17 (2019): 4193. http://dx.doi.org/10.3390/ijms20174193.

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Previously, we detected a higher degree of mineralization in fetal calf serum (FCS) compared to serum-free cultured jaw periosteum derived osteoprogenitor cells (JPCs). By Raman spectroscopy, we detected an earlier formation of mineralized extracellular matrix (ECM) of higher quality under serum-free media conditions. However, mineralization potential remained too low. In the present study, we aimed to investigate the biochemical composition and subsequent biomechanical properties of the JPC-formed ECM and minerals under human platelet lysate (hPL) and FCS supplementation. JPCs were isolated (

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12

George, F., C. Daniaux, G. Genicot, et al. "161 LIPID CONTENT, RESISTANCE TO CRYOPRESERVATION, AND SEX RATIO OF IN VITRO BOVINE BLASTOCYSTS PRODUCED IN A SERUM-FREE SYSTEM." Reproduction, Fertility and Development 18, no. 2 (2006): 188. http://dx.doi.org/10.1071/rdv18n2ab161.

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In vitro-produced (IVP) bovine blastocysts are known to be more sensitive to cryopreservation than their in vivo counterparts. Removing serum from the culture medium decreases sanitary risk and could improve embryo resistance to cryopreservation by preventing the accumulation of intracellular lipids. Our objectives were to evaluate the lipid content, resistance to cryopreservation, and sex ratio of IVP embryos cultured in a serum-free system. Oocytes from slaughterhouse ovaries were matured in a serum-free enriched medium (Donnay et al. 2004 Reprod. Fertil. Dev. 16, 274) and cultured in 5% O2

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13

Hirst, S. J., P. J. Barnes, and C. H. Twort. "PDGF isoform-induced proliferation and receptor expression in human cultured airway smooth muscle cells." American Journal of Physiology-Lung Cellular and Molecular Physiology 270, no. 3 (1996): L415—L428. http://dx.doi.org/10.1152/ajplung.1996.270.3.l415.

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The effect of recombinant platelet-derived growth factor (PDGF) isoforms (PDGF-AA, -BB and -AB) on mitogenesis of human cultured airway smooth muscle (ASM) was examined using the MTT-reduction assay and [3H]thymidine incorporation. Results were correlated with expression of PDGF receptor (PDGFR)-alpha and -beta subunits in the absence and presence of fetal calf serum (FCS). When FCS was absent PDGF-AB and -BB were potent mitogens, whereas PDGF-AA was weakly mitogenic, evoking <20% of the maximum response induced by the B-chain isoforms. When FCS (2.5%) was present, all PDGF isoforms stimula

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14

Schmetzer, H., R. Pelka-Fleischer, T. Kröll, W. Hiddemann, and V. Nüssler. "Dentritic cells in AML and MDS-comparison of different FCS-free culture conditions." European Journal of Cancer 37 (September 2001): S36. http://dx.doi.org/10.1016/s0959-8049(01)80396-8.

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15

Simon, Melinda, Bálint Major, Gabriella Vácz, et al. "The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche." Stem Cells International 2018 (April 16, 2018): 1–12. http://dx.doi.org/10.1155/2018/4854619.

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Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as “prolifer

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16

Moreno, D., A. Neira, L. Dubreil, et al. "80 DEVELOPMENT OF A SYNTHETIC MEDIUM FOR THE IN VITRO CULTURE OF BOVINE EMBRYOS." Reproduction, Fertility and Development 26, no. 1 (2014): 154. http://dx.doi.org/10.1071/rdv26n1ab80.

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In the majority of media for embryo culture, 2 of typical components used are FCS or BSA; however, the presence of FCS in the culture medium has been shown to have a negative effect on embryo quality and the use of animal-derived proteins in culture media increases the risks of disease transmission through in vitro embryo production. The aim of this study was to develop an in vitro embryo culture medium free from FCS and BSA, but with the addition of various growth factors and cytokines (GF-CYK: IGF-I, IGF-II, bFGF, LIF, GM-CSF) 50 ng mL–1 and (TGF-β1) 100 ng mL–1 supplemented with hyaluronan

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17

Nõmm, M., E. Mark, O. Sarv, S. Kõks, and Ü. Jaakma. "193 IMPROVED POST-THAW SURVIVAL OF BOVINE EMBRYOS PRODUCED IN SERUM-FREE IN VITRO PRODUCTION SYSTEM." Reproduction, Fertility and Development 28, no. 2 (2016): 227. http://dx.doi.org/10.1071/rdv28n2ab193.

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Over a few decades the bovine in vitro embryo production (IVP) systems have been improving rapidly. Still, the goal to produce the same quality embryos in vitro as in vivo has not yet been reached. The FCS is usually added to media during IVP to provide growth factors and energy sources. Currently, serum-free culture systems are often preferred due to the lower risk of contamination and prevention of the development of large offspring syndrome. The aim of this study was to establish whether complete elimination of FCS from the bovine IVP system has an effect on blastocyst rates, embryo quality

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18

Abeydeera, Lalantha R., Hiroaki Funahashi, Nam-Hyung Kim, and Billy N. Day. "Chlortetracycline fluorescence patterns and in vitro fertilisation of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations." Zygote 5, no. 2 (1997): 117–25. http://dx.doi.org/10.1017/s0967199400003798.

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SummaryPorcine oocyte-cumulus complexes were cultured in bovine serum albumin(BSA)-free North Carolina State University (NCSU)23 medium containing porcine follicular fluid (10%), cysteine (0.1 mg/ml)and hormonal supplements (eCG and hCG: 10IU/ml each) for 22h. They were then cultured in the same medium but without hormonal supplements for an additional 22h. After culture, cumulus cells were removed and oocytes were co-incubated with frozen-thawed ejaculated boar spermatozoa in tissue culture medium (TCM)199 containing caffeine (5mM), fetal calf serum (FCS; 10%) and varying concentrations (26–5

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Revelen, Ronan, Anne Bordron, Maryvonne Dueymes, Pierre Youinou, and Josiane Arvieux. "False Positivity in a Cyto-ELISA for Anti-Endothelial Cell Antibodies Caused by Heterophile Antibodies to Bovine Serum Proteins." Clinical Chemistry 46, no. 2 (2000): 273–78. http://dx.doi.org/10.1093/clinchem/46.2.273.

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Abstract Background: ELISAs with fixed endothelial cells or cell lines are widely used screening tests for anti-endothelial cell antibodies (AECAs), but spurious increases occur. We examined interferences by heteroantibodies and means to eliminate them. Methods: AECAs were measured by ELISA on fixed layers of the human endothelial cell line, EA.hy 926, in a panel of 60 patient serum samples diluted in bovine serum albumin. Heteroantibodies against fetal calf serum (FCS) proteins were demonstrated and characterized in an ELISA—the interference assay—that used FCS-coated plates and Tween 20-cont

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Caputcu, A. T., S. Arat, M. Cevik, T. Akkoc, G. Cetinkaya, and H. Bagis. "32 ALLOCATION OF INNER CELL MASS AND TROPHECTODERM CELLS OF NUCLEAR TRANSFER EMBRYOS CULTURED IN MEDIUM SUPPLEMENTED WITH EPIDERMAL GROWTH FACTOR AND INSULIN-LIKE GROWTH FACTOR-I." Reproduction, Fertility and Development 27, no. 1 (2015): 109. http://dx.doi.org/10.1071/rdv27n1ab32.

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This study was conducted to determine the additive effects of exogenous growth factors and different macromolecules during in vitro oocyte maturation (IVM) and sequential embryo culture of nuclear transfer (NT) embryos. Oocytes were matured in TCM-199 supplemented with 10% fetal calf serum (FCS), 50 mg mL–1 sodium pyruvate, 1% penicillin/streptomycin (10 000 U mL–1 penicillin G, 10 000 mg mL–1 streptomycin), 5 mg mL–1 LH, and 0.5 mg mL–1 FSH without growth factors (Treatment 1) or with 50 ng mL–1 epidermal growth factor (EGF; Treatment 2) or with 50 ng mL–1 EGF and 100 ng mL–1 insulin-like gro

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Ordoñez-Leon, E. A., G. Cancino, J. Hernandez-Ceron, J. A. Medrano, Y. C. Ducolomb, and S. Romo. "292 EVALUATION OF THREE PROTEIN SOURCES FOR THE IN VITRO PRODUCTION OF BOVINE EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 302. http://dx.doi.org/10.1071/rdv22n1ab292.

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Bovine embryo development in vitro can be affected by many factors, including protein source, which can cause embryo development failure. The use of in vitro culture media supplemented with serum-free compounds could allow a better understanding of embryo requirements during the preimplantation stages by eliminating a highly variable and undefined compound such as serum. The objective of this study was to evaluate the effect of 3 different protein supplements used during IVM, IVF, and IVC on embryo production. Ovaries were collected from slaughtered cows and then aspirated to obtain oocytes fo

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Valtieri, M., M. Gabbianelli, E. Pelosi, et al. "Erythropoietin alone induces erythroid burst formation by human embryonic but not adult BFU-E in unicellular serum-free culture [see comments]." Blood 74, no. 1 (1989): 460–70. http://dx.doi.org/10.1182/blood.v74.1.460.460.

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Abstract Erythroid progenitors (BFU-E) from adult human peripheral blood generate erythroid bursts in semisolid culture supplemented with at least two growth factors, ie, erythropoietin (Ep) and interleukin-3 (IL- 3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). We have analyzed the hematopoietin(s) requirement of human embryonic BFU- E, as compared to that of adult peripheral blood progenitors: This was basically evaluated in fetal calf serum-free (FCS-) methylcellulose culture of partially or highly purified progenitors treated with human recombinant hemopoietins. At a low se

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Valtieri, M., M. Gabbianelli, E. Pelosi, et al. "Erythropoietin alone induces erythroid burst formation by human embryonic but not adult BFU-E in unicellular serum-free culture [see comments]." Blood 74, no. 1 (1989): 460–70. http://dx.doi.org/10.1182/blood.v74.1.460.bloodjournal741460.

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Erythroid progenitors (BFU-E) from adult human peripheral blood generate erythroid bursts in semisolid culture supplemented with at least two growth factors, ie, erythropoietin (Ep) and interleukin-3 (IL- 3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). We have analyzed the hematopoietin(s) requirement of human embryonic BFU- E, as compared to that of adult peripheral blood progenitors: This was basically evaluated in fetal calf serum-free (FCS-) methylcellulose culture of partially or highly purified progenitors treated with human recombinant hemopoietins. At a low seeding con

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Hamilton-Valensky, Melisa, Vivian Lam, Judit Banáth, Gerald Krystal, and Kevin Bennewith. "Myeloid-derived suppressor cells isolated from 4T1 tumor-bearing mice are only immunosuppressive under serum-free conditions (147.25)." Journal of Immunology 186, no. 1_Supplement (2011): 147.25. http://dx.doi.org/10.4049/jimmunol.186.supp.147.25.

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Abstract Myeloid-derived suppressor cells (MDSCs) are defined by their ability to suppress T cell responses. However, different in vitro culture conditions have been used to assay MDSC function, which may explain why some labs have been unable to demonstrate the suppressive nature of these cells. To address this, we isolated MDSCs from BALB/c mice bearing syngeneic 4T1 metastatic murine mammary tumors and compared the ability of these 4T1-MDSCs to suppress T cell proliferation under serum-containing (10% FCS) and serum-free conditions. Interestingly, we found that these MDSCs only suppressed T

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Hamdi, Meriem, Ricaurte Lopera-Vasquez, Veronica Maillo, et al. "Bovine oviductal and uterine fluid support in vitro embryo development." Reproduction, Fertility and Development 30, no. 7 (2018): 935. http://dx.doi.org/10.1071/rd17286.

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In order to mimic the maternal oviductal environment, we evaluated the effect of oviductal fluid (OF) and/or uterine fluid (UF) supplementation on in vitro embryo development and quality. In vitro-produced zygotes were cultured with 1.25% OF from Day 1 to Day 4 after insemination (OF group), 1.25% OF from Day 1 to Day 4 followed by 1.25% UF from Day 4 to Day 9 (OF+UF group) or 1.25% UF only from Day 4 to Day 9 (UF group). Control groups were cultured in the presence of synthetic oviduct fluid (SOF) supplemented with 3 mg mL−1 bovine serum albumin (BSA) or 5% fetal calf serum (FCS). Supplementa

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Alexopoulos, Natalie I., Gábor Vajta, Poul Maddox-Hyttel, Andrew J. French, and Alan O. Trounson. "Stereomicroscopic and histological examination of bovine embryos following extended in vitro culture." Reproduction, Fertility and Development 17, no. 8 (2005): 799. http://dx.doi.org/10.1071/rd04104.

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Attempts to support survival of mammalian embryos after hatching have met with limited success, although some mouse studies have reported growth at the post-implantation stage. The aim of the present research was to establish and characterise an in vitro culture system that could support extended growth and differentiation of bovine embryos. Abattoir-derived oocytes were matured and fertilised in vitro. Presumptive zygotes were cultured in modified synthetic oviduct fluid (SOFaaci) medium supplemented with 5% cow serum (CS). On Day 9, single hatched blastocysts (n = 160) were randomly allocate

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Ikenouchi, H., L. Zhao, M. McMillan, E. M. Hammond, and W. H. Barry. "ATP depletion causes a reversible decrease in Na+ pump density in cultured ventricular myocytes." American Journal of Physiology-Heart and Circulatory Physiology 264, no. 4 (1993): H1208—H1214. http://dx.doi.org/10.1152/ajpheart.1993.264.4.h1208.

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To examine factors contributing to impaired K+ homeostasis induced by prolonged but sublethal ATP depletion, we subjected cultured chick ventricular myocytes to metabolic inhibition with 20 mM 2-deoxy-D-glucose plus 1 mM NaCN for 2 h and then allowed myocytes to recover for 5 days in medium containing 6% fetal calf serum (FCS) or in hormone-supplemented serum-free medium. We measured spontaneous contractions (with a video motion detector), K+ content, K+ uptake, membrane potential, and Na+ pump density ([3H]ouabain binding). Exposure to metabolic inhibition for 2 h caused an acute decrease in

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Zhang, S., A. J. French, and R. T. Tecirlioglu. "255. In vitro maturation of bovine oocytes in serum-free media." Reproduction, Fertility and Development 17, no. 9 (2005): 102. http://dx.doi.org/10.1071/srb05abs255.

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Culture medium supplemented with sera is commonly used for the in vitro production (IVP) of livestock embryos. However, serum induced complications including batch variation, the potential risk of virus and mycoplasma contamination and the implication in the large offspring syndrome in domestic animals impels the development of a serum-free culture system. In this study, we investigated whether replacement of fetal bovine serum (FBS) with bovine serum albumin (BSA) in three maturation media, tissue culture medium-199 (TCM-199), a modified synthetic oviduct fluid (mSOF) routinely used in our la

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Wani, N. A., J. A. Skidmore, and U. Wernery. "55 PRELIMINARY STUDIES ON THE DEVELOPMENT OF RECONSTRUCTED EMBRYOS AFTER NUCLEAR TRANSFER IN DROMEDARY CAMEL (CAMELUS DROMEDARIUS)." Reproduction, Fertility and Development 21, no. 1 (2009): 128. http://dx.doi.org/10.1071/rdv21n1ab55.

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Experiments were conducted to study the in vitro development of reconstructed dromedary camel embryos after nuclear transfer by a modified zona-free method. Cumulus oocyte complexes, collected from slaughterhouse ovaries were cultured in TCM199 at 38.5°C in an atmosphere of 5% CO2 in air for 32 to 36 h. Matured oocytes were denuded of cumulus cells by repeated pipetting and the zona pellucida was removed by brief incubation in 5 mg mL–1 pronase dissolved in Ca- and Mg-free PBS. Zona-free oocytes were stained with 5 mg mL–1 Hoechst 33342 in H199 supplemented with 7.5 μg mL–1 cytochalasin B and

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Wen, Yujie, Yiming Huang, Thomas O. Miller, Mariusz Z. Ratajczak, and Suzanne T. Ildstad. "CD8+TCR- Bone Marrow Facilitating Cells Prime Migration and Enhance Homing of Hematopoietic Stem Cells." Blood 124, no. 21 (2014): 2412. http://dx.doi.org/10.1182/blood.v124.21.2412.2412.

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Abstract CD8+TCR- bone marrow facilitating cells (FCs) facilitate engraftment of hematopoietic stem cells (HSCs) in both allogeneic and syngeneic recipients. We recently reported that co-administration of FCs with HSCs established chimerism and induced tolerance to renal allografts without graft-versus-host disease and engraftment syndrome in HLA-mismatched living donor renal transplant recipients. Homing of HSCs to the bone marrow niche is believed to be a crucial prerequisite for engraftment. Therefore, we evaluated whether FCs enhance functional HSC homing and retention in the hematopoietic

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Chen, Y., M. Allars, C. Abou-Seif, and R. C. Nicholson. "110. EFFECTS OF cAMP AND SERUM ON HUMAN TROPHOBLAST CELL VIABILITY AND DIFFERENTIATION." Reproduction, Fertility and Development 21, no. 9 (2009): 29. http://dx.doi.org/10.1071/srb09abs110.

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The formation of syncytium is a pivotal event for trophoblast cells to interact with the placental bed. While cAMP is regarded as an inducer of syncytialisation, the affect of different culture conditions on this cAMP effect has not been explored. Therefore, the effects of cAMP on cell differentiation and viability in the presence or absence of serum were investigated in the human choriocarcinoma cell lines, BeWo and JEG-3. We observed that in the absence of cAMP, BeWo cells grew best in media containing 10% FCS, followed by media containing charcoal-stripped 10% FCS (10%CCS), and less well in

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Triwulanningsih, Endang, M. R. Toelihere, J. J. Rutledge, T. L. Yusuf, B. Purwantara, and K. Diwyanto. "Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle." Jurnal Ilmu Ternak dan Veteriner 7, no. 1 (2014): 30–37. https://doi.org/10.14334/jitv.v7i1.272.

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This research has been conducted at the laboratory of in vitro fertilization in the Department of Animal Science University of Wisconsin, USA. These embryos can be used for improving genetic value of Indonesian cattle. Oocytes were matured in TCM- 199 medium (in 5% CO2 incubator and at 390C) enriched with follicle stimulating hormone (FSH) 10 Î¼l/ml, oestradiol 17 Î² 1Î¼l/ml and 10% Fetal Calf Serum (FCS). The oocytes were fertilized in vitro with motile sperm and incubation between sperm and oocytes in fertilization medium Tyroide Albumin Lactate Pyruvate (TALP) for 20 hours. All zygotes were

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Clavreul, Anne, Isabelle Jean, Laurence Preisser, et al. "Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy." In Vitro Cellular & Developmental Biology - Animal 45, no. 9 (2009): 500–511. http://dx.doi.org/10.1007/s11626-009-9215-4.

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Todorova, Tsvetelina, and Tania Todorova. "Effect of follicle size and culture media on in vitro maturation of juvenile porcine oocytes." Bulgarian Journal of Animal Husbandry 60, no. 5 (2023): 12–21. http://dx.doi.org/10.61308/tepf2324.

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In vitro maturation (IVM) of oocytes is a critical step in the assisted reproductive technologies performed in pigs. The aim of the present study was to determine what effect the use of a particular maturation medium has on the maturation of oocytes from follicles of different sizes. The results of SF and LF oocytes cultured in medium containing FCS after 0, 28 and 46 h of IVM were compared. Before the start of culture (0 h), 92% of oocytes from both classes of follicles were in the GV stage and after the first half of IVM (28 h). At the end of culture (46 h), both groups of oocytes completed

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Bernardo, Maria Ester, Maria Antonietta Avanzini, Cesare Perotti, et al. "Platelet-Lysate for In Vitro Expansion of Human Multipotent Mesenchymal Stromal Cells in Approaches of Cell-Therapy." Blood 108, no. 11 (2006): 2577. http://dx.doi.org/10.1182/blood.v108.11.2577.2577.

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Abstract There is large interest in the use of mesenchymal stromal cells (MSCs) in approaches of cell-therapy and tissue engineering. MSCs are currently expanded in vitro in the presence of fetal calf serum (FCS); however, FCS raises concerns in the case of clinical grade cellular preparations because of the theoretical risk of transmission of zoonoses and triggering immune reactions in the host. Therefore, the identification of a serum-free medium appropriate for both the extensive expansion necessary to reach the large numbers of MSCs required for clinical application, and the exclusion of r

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Quarto, R., G. Campanile, R. Cancedda, and B. Dozin. "Thyroid hormone, insulin, and glucocorticoids are sufficient to support chondrocyte differentiation to hypertrophy: a serum-free analysis." Journal of Cell Biology 119, no. 4 (1992): 989–95. http://dx.doi.org/10.1083/jcb.119.4.989.

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Chondrocytes from chicken embryo tibia can be maintained in culture as adherent cells in Coon's modified Ham's F-12 medium supplemented with 10% FCS. In this condition, they dedifferentiate, losing type II collagen expression in favor of type I collagen synthesis. Their differentiation to hypertrophy can be obtained by transferring them to suspension culture. Differentiation is evidenced by the shift from type I to type II and type IX collagen synthesis and the following predominant expression of type X collagen, all markers of specific stages of the differentiation process. To identify the fa

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Rutten, M. J., P. J. Dempsey, T. E. Solomon, and R. J. Coffey. "TGF-alpha is a potent mitogen for primary cultures of guinea pig gastric mucous epithelial cells." American Journal of Physiology-Gastrointestinal and Liver Physiology 265, no. 2 (1993): G361—G369. http://dx.doi.org/10.1152/ajpgi.1993.265.2.g361.

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Transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF) are thought to be important in gastric epithelial proliferation and repair. It was therefore of interest to determine if TGF-alpha and EGF promoted the growth of an in vitro primary culture system of guinea pig gastric mucous epithelial cells (MEC). MEC were isolated from guinea pig stomachs and cultured in 24-well Primaria plates with DMEM with or without 10% fetal calf serum (FCS). Growth of MEC was determined by changes in [3H]thymidine uptake, cell counts, protein, and DNA. The sources of peptides were human rec

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Serapião, R. V., L. S. de Almeida Camargo, A. de Almeida Ramos, et al. "280 EXPRESSION OF HSP70.1 GENE IN IN VITRO-PRODUCED BOVINE EMBRYOS CULTURED IN CR2 MEDIUM SUPPLEMENTED WITH KNOCKOUT™SR." Reproduction, Fertility and Development 19, no. 1 (2007): 255. http://dx.doi.org/10.1071/rdv19n1ab280.

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The exposure of embryos to serum during in vitro culture can affect morphology, metabolism, tolerance to cryopreservation, and expression of specific transcripts. On the other hand, serum-free medium seems to avoid some of those serum effects. KnockoutTMSR (GIBCO Laboratories, Grand Island, NY, USA) is a serum replacer optimized to support embryonic stem cells in culture and can also be used to replace serum during culture of in vitro-fertilized bovine embryos. The expression of genes associated with stress response, such as heat shock proteins (HSP), can be affected by in vitro culture condit

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Hopfer, Helmut, Günter Vollmer, Clifford A. Rinehart Jr., and David G. Kaufman. "Basement membrane induced differentiation of HEC-1B(L) endometrial adenocarcinoma cells affects both morphology and gene expression." Biochemistry and Cell Biology 74, no. 2 (1996): 165–77. http://dx.doi.org/10.1139/o96-017.

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In vitro studies of endometrial carcinogenesis have been hampered by dedifferentiation of the cells in culture. Using the endometrial carcinoma cell line HEC-1B(L), we aimed to establish and characterize culture conditions mat preserve a more differentiated state of the tumor cells. HEC-1B(L) cells grown in a serum-free defined medium on plastic (PL/SFDM) on top of a reconstituted basement membrane (Matrigel™, MG/SFDM) or in a thick layer of Matrigel showed pronounced morphological differentiation as compared with HEC-1B(L) cells cultured on plastic in a medium containing serum (PL/10% FCS). F

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Matoba, S., and P. Lonergan. "120 EFFECT OF INDIVIDUAL CULTURE SYSTEM USING WOW OR CELL-TAK ON DEVELOPMENT OF IN VITRO-PRODUCED BOVINE EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 160. http://dx.doi.org/10.1071/rdv21n1ab120.

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The culture of embryos individually in vitro is generally associated with poorer developmental rates. However, the ability to do this successfully would greatly facilitate studies where identification of individual embryos, or the embryos from a particular donor, is necessary. The objective of this study was to examine the effect of culture system on the development of individual IVP bovine embryos. Presumptive zygotes (n = 1301, 6 replicates), produced by IVM/IVF, were used. The aim of Experiment 1 was to compare development of bovine embryos in SOF or CR1aa supplemented with 5% FCS. Zygotes

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Kuvibidila, Solo R., Maria Velez, Lolie Yu, Raj P. Warrier, and B. Surendra Baliga. "Differences in iron requirements by concanavalin A-treated and anti-CD3-treated murine splenic lymphocytes." British Journal of Nutrition 88, no. 1 (2002): 67–72. http://dx.doi.org/10.1079/bjn2002576.

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Fe availability is critical for optimal lymphocyte proliferation; however, the minimum required levels are unknown. Such information is valuable when assessingin vitroimmune responses in Fe-deficient subjects, because serum (Fe) added to the culture medium may replete lymphocytes. To address this issue, splenic lymphocytes obtained from seventeen 3-month-old C57BL/6 mice were incubated without and with 1 mg/l concanavalin A or 50 μg/l anti-CD3 antibody in media that contained between 0·113 and 9·74 μmol Fe/l. Fe was provided by either fetal calf serum (FCS, 0–100 ml/l), newborn calf serum (NBC

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Rajapakse, Dinusha, Tim Curtis, Mei Chen, and Heping Xu. "Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture." Oxidative Medicine and Cellular Longevity 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/6926485.

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Zinc deficiency is known to increase the risk of the development of age-related macular degeneration (AMD), although the underlying mechanism remains poorly defined. In this study, we investigated the effect of zinc on retinal pigment epithelium (RPE) survival and function under oxidative conditions. Zinc level was 5.4 μM in normal culture conditions (DMEM/F12 with 10% FCS) and 1.5 μM in serum-free medium (DMEM/F12). Under serum-free culture conditions, the treatment of RPE cells with oxidized photoreceptor outer segment (oxPOS) significantly increased intracellular ROS production, reduced ATP

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Reis, A., J. A. Rooke, G. J. McCallum, et al. "Consequences of exposure to serum, with or without vitamin E supplementation, in terms of the fatty acid content and viability of bovine blastocysts produced in vitro." Reproduction, Fertility and Development 15, no. 5 (2003): 275. http://dx.doi.org/10.1071/rd03004.

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To determine whether serum supplementation influenced fatty acid content of bovine blastocysts and whether vitamin E addition to culture medium containing serum could improve development in vitro, cleaved eggs were cultured in synthetic oviduct fluid supplemented with bovine serum albumin (BSA, 0.4% w/v, fraction V) (SVBSA), fetal calf serum (FCS, 10% v/v) (SFCS) or FCS (10% v/v) plus 100 μM vitamin E (SFCS + E). Blastocyst yields were recorded and fatty acid composition was determined by gas chromatography. Day 7 blastocysts were incubated with [2-14C] pyruvate for 3 h and then fixed for cell

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Yoshimura, T., and E. J. Leonard. "Secretion by human fibroblasts of monocyte chemoattractant protein-1, the product of gene JE." Journal of Immunology 144, no. 6 (1990): 2377–83. http://dx.doi.org/10.4049/jimmunol.144.6.2377.

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Abstract We recently purified human monocyte chemoattractant protein-1 (MCP-1) from culture fluids of either human glioma cell lines or mitogen-stimulated human peripheral blood mononuclear leukocytes. It has now been shown that MCP-1 is the product of the gene JE, which was first recognized by its expression in fibroblasts stimulated with platelet-derived growth factor (PDGF). We therefore studied secretion of MCP-1 by three human fibroblast cell lines. Monocyte chemotactic activity was found in culture fluids of all three lines after growth to confluence in DMEM-10% FCS, and the amounts secr

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Maziero, R. R. D., D. M. Paschoal, M. D. Guastali, et al. "162 EFFECT OF MEIOTIC ARREST USING BUTYROLACTONE I AND ROSCOVITINE IN RESISTANCE TO EMBRYO CRYOPRESERVATION." Reproduction, Fertility and Development 26, no. 1 (2014): 195. http://dx.doi.org/10.1071/rdv26n1ab162.

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In vitro embryo production by maturation, fertilization, and culture has become a valuable assisted reproductive technology in cattle breeding systems. However, even with the notable innovations in this system, the greatest obstacles for the complete success of this biotechnology are the low pregnancy rates after transfer and the greater sensitivity to cryopreservation. Thus, this study evaluated the effect of the addition of a meiotic arrest using butyrolactone I and roscovitine on in vitro production of bovine embryos. Nelore oocytes were matured in TCM-199 with Earle's salt + 10% FCS, FSH,

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46

Polak-Charcon, Sylvie, Mehrdad Hekmati, and Yehuda Ben Shaul. "Ultrastructural Study of a differentiating human colon carcinoma cell line." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 208–9. http://dx.doi.org/10.1017/s0424820100158583.

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The epithelium of normal human colon mucosa “in vivo” exhibits a gradual pattern of differentiation as undifferentiated stem cells from the base of the crypt of “lieberkuhn” rapidly divide, differentiate and migrate toward the free surface. The major differentiated cell type of the intestine observed are: absorptive cells displaying brush border, goblet cells containing mucous granules, Paneth and endocrine cells containing dense secretory granules. These different cell types are also found in the intestine of the 13-14 week old embryo.We present here morphological evidence showing that HT29,

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Ichii, Michiko, Kenji Oritani, Takafumi Yokota, et al. "Establishment of Stroma-Free Cultures for Human B Lymphopoiesis: Roles of High Cell Density Condition and Mesenchymal Stem Cell-Secreted Factors." Blood 110, no. 11 (2007): 1420. http://dx.doi.org/10.1182/blood.v110.11.1420.1420.

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Abstract Background: Due to lacking of appropriate culture systems, it has been difficult to analyze mechanisms of human B lymphocytes. However, we recently found that human mesenchymal stem cells (MSC) have high ability to support human B lymphopoiesis. Although many investigators have believed that stromal layers are essential for supporting the differentiation of human B lymphocyte progenitors, this hypothesis is not a case. By manipulating our co-culture system, we have successfully established stroma-free suspension cultures, which produce CD10+ B cells from human umbilical cord blood (CB

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48

Sonoda, Y., YC Yang, GG Wong, SC Clark, and M. Ogawa. "Erythroid burst-promoting activity of purified recombinant human GM-CSF and interleukin-3: studies with anti-GM-CSF and anti-IL-3 sera and studies in serum-free cultures." Blood 72, no. 4 (1988): 1381–86. http://dx.doi.org/10.1182/blood.v72.4.1381.1381.

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Abstract We studied the erythroid burst-promoting activity (BPA) of recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF) and Interleukin-3 (IL-3) with two experimental approaches. First we studied the effects of polyclonal antisera prepared against human GM-CSF and gibbon IL-3 on colony formation from 1,000 bone marrow null cells/dish in serum-containing culture. Both GM-CSF and IL-3 independently enhanced erythroid burst formation; however, IL-3 showed more BPA activity than GM-CSF. These data are in agreement with an emerging view that the primary targets of IL-3 are p

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49

Sonoda, Y., YC Yang, GG Wong, SC Clark, and M. Ogawa. "Erythroid burst-promoting activity of purified recombinant human GM-CSF and interleukin-3: studies with anti-GM-CSF and anti-IL-3 sera and studies in serum-free cultures." Blood 72, no. 4 (1988): 1381–86. http://dx.doi.org/10.1182/blood.v72.4.1381.bloodjournal7241381.

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We studied the erythroid burst-promoting activity (BPA) of recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF) and Interleukin-3 (IL-3) with two experimental approaches. First we studied the effects of polyclonal antisera prepared against human GM-CSF and gibbon IL-3 on colony formation from 1,000 bone marrow null cells/dish in serum-containing culture. Both GM-CSF and IL-3 independently enhanced erythroid burst formation; however, IL-3 showed more BPA activity than GM-CSF. These data are in agreement with an emerging view that the primary targets of IL-3 are primitive

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Valk, Peter, Sandra Verbakel, Yolanda Vankan, et al. "Anandamide, a Natural Ligand for the Peripheral Cannabinoid Receptor Is a Novel Synergistic Growth Factor for Hematopoietic Cells." Blood 90, no. 4 (1997): 1448–57. http://dx.doi.org/10.1182/blood.v90.4.1448.

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Abstract We recently demonstrated that the gene encoding the peripheral cannabinoid receptor (Cb2) may be a proto-oncogene involved in murine myeloid leukemias. We show here that Cb2 may have a role in hematopoietic development. RNAse protection analysis showed that Cb2 is normally expressed in spleen and thymus. Cb2 mRNA is also expressed in 45 of 51 cell lines of distinct hematopoietic lineages, ie, myeloid, macrophage, mast, B-lymphoid, T-lymphoid, and erythroid cells. The effect of the fatty acid anandamide, an endogenous ligand for cannabinoid receptors, on primary murine marrow cells and

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