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1

Huang, Tao, Wilfred M. Wollheim, and Stephen H. Jones. "Removal of Fecal Indicator Bacteria by River Networks." Water 14, no. 4 (2022): 617. http://dx.doi.org/10.3390/w14040617.

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Fecal contamination is a significant source of water quality impairment globally. Aquatic ecosystems can provide an important ecosystem service of fecal contamination removal. Understanding the processes that regulate the removal of fecal contamination among river networks across flow conditions is critical. We applied a river network model, the Framework for Aquatic Modeling in the Earth System (FrAMES-Ecoli), to quantify removal of fecal indicator bacteria by river networks across flow conditions during summers in a series of New England watersheds of different characteristics. FrAMES-Ecoli
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2

COOK, DAVID W., and R. D. ELLENDER. "Relaying to Decrease the Concentration of Oyster-Associated Pathogens." Journal of Food Protection 49, no. 3 (1986): 196–202. http://dx.doi.org/10.4315/0362-028x-49.3.196.

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Oysters experimentally contaminated with indicator bacteria, Salmonella and poliovirus were used in relaying studies designed to measure microbial elimination under a variety of environmental conditions. Two factors, level of microorganism in the oyster and temperature of the water, were important in determining the length of time necessary to purge the contaminating organisms. Oysters under physiological stress cleansed at a slower rate than did healthy oysters. Based on the expected level of pathogen contamination in naturally polluted oysters, healthy relaid oysters were capable of cleansin
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3

Stewart, J. R., M. D. Sobsey, T. M. Gambling, and J. L. Carson. "Morphological Identification of Indicator Viruses from a Ground Water Aquifer Recharged with Reclaimed Wastewater." Microscopy and Microanalysis 4, S2 (1998): 500–501. http://dx.doi.org/10.1017/s1431927600022625.

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Protection of public health requires routine monitoring of environmental waters for fecal contamination, especially waters serving as drinking supplies. Municipalities typically rely on assays of indicator microorganisms to detect fecal contamination. Ideally, indicators and pathogens share a common origin. However, the indicators should have higher numbers, survive longer, and be easier to detect and quantify in the laboratory than pathogens. Coliform bacteria are the standard microbial indicator currently used in the United States. Enteric viruses are smaller and survive longer than coliform
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4

Afianti, Nur Fitriah, Lies Indah Sutiknowati, and Risky Ayu Kristanti. "ABUNDANCE OF POLLUTION-INDICATOR BACTERIA IN CIMANUK WATERS, INDRAMAYU." Suranaree Journal of Science and Technology 31, no. 6 (2025): 030235(1–7). https://doi.org/10.55766/sujst-2024-06-e04798.

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Cimanuk waters are one of Indonesia’s areas with abundant natural resource potential that have been threatened with contamination due to impulsive population growth and high human activities. Microbiological aspects are one of the parameters that can indicate environmental pollution. Therefore, pollution-indicator bacteria such as coliform and pathogenic bacteria have become one of the most important indicators of environmental conditions. This study conducted an analysis of pollution indicator bacteria at several sampling sites around the Cimanuk estuary to assess the environmental status. Su
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5

Rosario, Karyna, Erin M. Symonds, Christopher Sinigalliano, Jill Stewart, and Mya Breitbart. "Pepper Mild Mottle Virus as an Indicator of Fecal Pollution." Applied and Environmental Microbiology 75, no. 22 (2009): 7261–67. http://dx.doi.org/10.1128/aem.00410-09.

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ABSTRACT Accurate indicators of fecal pollution are needed in order to minimize public health risks associated with wastewater contamination in recreational waters. However, the bacterial indicators currently used for monitoring water quality do not correlate with the presence of pathogens. Here we demonstrate that the plant pathogen Pepper mild mottle virus (PMMoV) is widespread and abundant in wastewater from the United States, suggesting the utility of this virus as an indicator of human fecal pollution. Quantitative PCR was used to determine the abundance of PMMoV in raw sewage, treated wa
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6

Naphtali, Paul, Mahi M. Mohiuddin, Athanasios Paschos, and Herb E. Schellhorn. "Application of high-throughput 16S rRNA sequencing to identify fecal contamination sources and to complement the detection of fecal indicator bacteria in rural groundwater." Journal of Water and Health 17, no. 3 (2019): 393–403. http://dx.doi.org/10.2166/wh.2019.295.

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Abstract Residents in rural communities across Canada collect potable water from aquifers. Fecal contaminants from sewage and agricultural runoffs can penetrate aquifers, posing a public health risk. Standard methods for detecting fecal contamination test for fecal indicator bacteria (FIB), but the presence of these do not identify sources of contamination. In contrast, DNA-based diagnostic tools can achieve this important objective. We employed quantitative polymerase chain reaction (qPCR) and high-throughput DNA sequencing to trace fecal contamination sources in Wainfleet, a rural Ontario to
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7

Zarić, G., S. Cocoli, D. Šarčević, et al. "Escherichia coli as Microbiological Quality Water Indicator:A High Importance for Human and Animal Health." Journal of the Hellenic Veterinary Medical Society 74, no. 3 (2023): 6117–24. http://dx.doi.org/10.12681/jhvms.30878.

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The purpose of this review is to demonstrate the importance of monitoring microbiological quality in water for animals and humans, which relies primarily on coliforms, mostly Escherichia coli. Fecal coliforms, such as E. coli, are more specific indicators of fecal pollution. A fecal contamination detection can be performed not just in drinking water, but also in the environment. As a result of improved detection methods for E. coli, drinking water is becoming more reliable as a result of the use of E. coli as an indicator of fecal pollution. Drinking water contamination by feces is currently b
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8

Newton, Ryan J., Jessica L. VandeWalle, Mark A. Borchardt, Marc H. Gorelick, and Sandra L. McLellan. "Lachnospiraceae and Bacteroidales Alternative Fecal Indicators Reveal Chronic Human Sewage Contamination in an Urban Harbor." Applied and Environmental Microbiology 77, no. 19 (2011): 6972–81. http://dx.doi.org/10.1128/aem.05480-11.

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ABSTRACTThe complexity of fecal microbial communities and overlap among human and other animal sources have made it difficult to identify source-specific fecal indicator bacteria. However, the advent of next-generation sequencing technologies now provides increased sequencing power to resolve microbial community composition within and among environments. These data can be mined for information on source-specific phylotypes and/or assemblages of phylotypes (i.e., microbial signatures). We report the development of a new genetic marker for human fecal contamination identified through microbial p
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9

Santiago-Rodriguez, Tasha M., Gary A. Toranzos, and Javier A. Arce-Nazario. "Assessing the microbial quality of a tropical watershed with an urbanization gradient using traditional and alternate fecal indicators." Journal of Water and Health 14, no. 5 (2016): 796–807. http://dx.doi.org/10.2166/wh.2016.041.

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Urbanization affects the microbial loading into tropical streams, but its impact on water quality varies across watersheds. Rainfall in tropical environments also complicates microbial dynamics due to high seasonal and annual variations. Understanding the dynamics of fecal contamination in tropical surface waters may be further hindered by limitations from the utilization of traditional microbial indicators. We measured traditional (Enterococcus spp. and Escherichia coli), as well as alternate (enterophages and coliphages) indicators of fecal contamination in a tropical watershed in Puerto Ric
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10

Martins, César de Castro, Rosalinda Carmela Montone, Rosa Carvalho Gamba, and Vivian Helena Pellizari. "Sterols and fecal indicator microorganisms in sediments from Admiralty Bay, Antarctica." Brazilian Journal of Oceanography 53, no. 1-2 (2005): 1–12. http://dx.doi.org/10.1590/s1679-87592005000100001.

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Sediments from the proximity of Ferraz station outfall, located in Admiralty Bay, Antarctica, were analyzed for fecal indicator microorganisms and sterols during the austral summer of 1999/2000 in order to assess human sewage input. Concentrations of total sterols and coprostanol ranged from 0.09 to 19.6 ¼g.g-1 and < 0.01 and 14.0 ¼g.g-1 dry weight (dw), respectively. Our analyses indicated that only sites within to 400m of the sewage outfall exhibited a sterol signal characteristic of human sewage input. Low levels of coprostanol and high levels of cholesterol in distant sites could be att
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11

Chavarria, Karina, Jorge Batista, and Kristin Saltonstall. "Widespread occurrence of fecal indicator bacteria in oligotrophic tropical streams. Are common culture-based coliform tests appropriate?" PeerJ 12 (September 6, 2024): e18007. http://dx.doi.org/10.7717/peerj.18007.

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Monitoring of stream water quality is a key element of water resource management worldwide, but methods that are commonly used in temperate habitats may not be appropriate in humid tropical systems. We assessed the influence of four land uses on microbial water quality in 21 streams in the Panama Canal Watershed over a one-year period, using a common culture-based fecal indicator test and 16S rDNA metabarcoding. Each stream was located within one of four land uses: mature forest, secondary forest, silvopasture, and traditional cattle pasture. Culturing detected total coliforms and Escherichia
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12

Kirschner, Alexander K. T., Thomas C. Zechmeister, Gerhard G. Kavka, et al. "Integral Strategy for Evaluation of Fecal Indicator Performance in Bird-Influenced Saline Inland Waters." Applied and Environmental Microbiology 70, no. 12 (2004): 7396–403. http://dx.doi.org/10.1128/aem.70.12.7396-7403.2004.

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ABSTRACT Wild birds are an important nonpoint source of fecal contamination of surface waters, but their contribution to fecal pollution is mostly difficult to estimate. Thus, to evaluate the relation between feces production and input of fecal indicator bacteria (FIB) into aquatic environments by wild waterfowl, we introduced a new holistic approach for evaluating the performance of FIB in six shallow saline habitats. For this, we monitored bird abundance, fecal pellet production, and the abundance of FIB concomitantly with a set of environmental variables over a 9-month period. For estimatin
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13

Pettus, Paul, Eugene Foster, and Yangdong Pan. "Predicting fecal indicator organism contamination in Oregon coastal streams." Environmental Pollution 207 (December 2015): 68–78. http://dx.doi.org/10.1016/j.envpol.2015.08.025.

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14

Shanks, Orin C., Christopher Nietch, Michael Simonich, Melissa Younger, Don Reynolds, and Katharine G. Field. "Basin-Wide Analysis of the Dynamics of Fecal Contamination and Fecal Source Identification in Tillamook Bay, Oregon." Applied and Environmental Microbiology 72, no. 8 (2006): 5537–46. http://dx.doi.org/10.1128/aem.03059-05.

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ABSTRACT The objectives of this study were to elucidate spatial and temporal dynamics in source-specific Bacteroidales 16S rRNA genetic marker data across a watershed; to compare these dynamics to fecal indicator counts, general measurements of water quality, and climatic forces; and to identify geographic areas of intense exposure to specific sources of contamination. Samples were collected during a 2-year period in the Tillamook basin in Oregon at 30 sites along five river tributaries and in Tillamook Bay. We performed Bacteroidales PCR assays with general, ruminant-source-specific, and huma
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15

Krolik, Julia, Allison Maier, Shawna Thompson, and Anna Majury. "Microbial source tracking of private well water samples across at-risk regions in southern Ontario and analysis of traditional fecal indicator bacteria assays including culture and qPCR." Journal of Water and Health 14, no. 6 (2016): 1047–58. http://dx.doi.org/10.2166/wh.2016.024.

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Many people living in rural areas rely on privately owned wells as their primary source of drinking water. These water sources are at risk for fecal contamination of human, wildlife, and livestock origin. While traditional bacteriological testing involves culture-based methods, microbial source tracking (MST) assays present an opportunity to additionally determine the source of fecal contamination. This study investigated the main host sources of contamination in private well water samples with high levels of Escherichia coli (E. coli), using MST with human and multi-species specific markers.
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16

Cha, Sung Min, Seung Won Lee, Yong Eun Park, Kyung Hwa Cho, Seungyoon Lee, and Joon Ha Kim. "Spatial and temporal variability of fecal indicator bacteria in an urban stream under different meteorological regimes." Water Science and Technology 61, no. 12 (2010): 3102–8. http://dx.doi.org/10.2166/wst.2010.261.

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As a representative urban stream in Korea, the Gwangju (GJ) stream suffers from chronic fecal contamination. In this study, to characterize levels of fecal pollution in the GJ stream, the monthly monitoring data for seven years (from 2001 to 2007) and the hourly monitoring data from two field experiments were examined with respect to seasonal/daily variations and spatial distribution under wet and dry weather conditions. This research revealed that concentrations of fecal indicator bacteria strongly varied depending on the prevalent meteorological conditions. That is, during the dry daytime, f
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17

MERINO-MASCORRO, JOSE ANGEL, LINDA G. HERNÁNDEZ-RANGEL, NORMA HEREDIA, and SANTOS GARCÍA. "Bacteroidales as Indicators and Source Trackers of Fecal Contamination in Tomatoes and Strawberries." Journal of Food Protection 81, no. 9 (2018): 1439–44. http://dx.doi.org/10.4315/0362-028x.jfp-18-073.

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ABSTRACT Most methods that investigate fecal contamination of vegetables do not address the origin of contamination. Because host-specific sequences are conserved in their genomes, bacteria of the order Bacteroidales are regarded as alternative indicators for tracking sources of contamination of produce. The objective of this study was to determine the efficacy of host-specific Bacteroidales markers to identify sources of fecal contamination and to determine whether detection of Bacteroidales markers correlated with traditional fecal indicator bacteria (FIB) in strawberries and tomatoes. Tomat
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18

Oliveira, M., D. Freire, and N. M. Pedroso. "Escherichia coli is not a suitable fecal indicator to assess water fecal contamination by otters." Brazilian Journal of Biology 78, no. 1 (2017): 155–59. http://dx.doi.org/10.1590/1519-6984.167279.

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Abstract The detection of pathogenic microorganisms in aquatic environments is extremely relevant in terms of public health. As these laboratorial methodologies are usually difficult, expensive and time-consuming, they are frequently replaced by the assessment of fecal indicator bacteria, such as Escherichia coli. This study aimed to assess the presence of E. coli in fecal samples from Neotropical otters, to evaluate its potential as fecal indicator to be applied to the determination of water microbiological quality in areas where otters’ populations are high. Twenty-six otter fecal samples, c
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19

Ley, Victoria, James Higgins, and Ronald Fayer. "Bovine Enteroviruses as Indicators of Fecal Contamination." Applied and Environmental Microbiology 68, no. 7 (2002): 3455–61. http://dx.doi.org/10.1128/aem.68.7.3455-3461.2002.

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ABSTRACT Surface waters frequently have been contaminated with human enteric viruses, and it is likely that animal enteric viruses have contaminated surface waters also. Bovine enteroviruses (BEV), found in cattle worldwide, usually cause asymptomatic infections and are excreted in the feces of infected animals in large numbers. In this study, the prevalence and genotype of BEV in a closed herd of cattle were evaluated and compared with BEV found in animals in the immediate environment and in environmental specimens. BEV was found in feces from 76% of cattle, 38% of white-tailed deer, and one
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20

Vithanage, Gayatri, Roger S. Fujioka, and Gary Ueunten. "Innovative Strategy Using Alternative Fecal Indicators (F+RNA/Somatic Coliphages, Clostridium perfringens) to Detect Cesspool Discharge Pollution in Streams and Receiving Coastal Waters Within a Tropical Environment." Marine Technology Society Journal 45, no. 2 (2011): 101–11. http://dx.doi.org/10.4031/mtsj.45.2.12.

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AbstractStandards based on fecal indicator bacteria (FIB) have traditionally been used by the U.S. Environmental Protection Agency to determine when recreational waters are contaminated by fecal and sewage inputs. Studies in tropical and subtropical areas, however, have shown that these same standards cannot reliably be used in these environments to determine when waters are contaminated by sewage. This is because soil, sediments, water, and plants have been shown to be significant indigenous sources of FIB in tropical/subtropical climates. In these climates, alternative fecal indicators, such
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GIOMBELLI, AUDECIR, and MARIA BEATRIZ ABREU GLORIA. "Prevalence of Salmonella and Campylobacter on Broiler Chickens from Farm to Slaughter and Efficiency of Methods To Remove Visible Fecal Contamination." Journal of Food Protection 77, no. 11 (2014): 1851–59. http://dx.doi.org/10.4315/0362-028x.jfp-14-200.

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A study was conducted to investigate the prevalence of Salmonella and Campylobacter from farm to slaughter. The efficiency of trimming and water spray (490 to 588 kPa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. Drag swabs were used to sample litter from the farm houses. Samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken during slaughter of the flocks previously visited at the farms. There was a low prevalence of Salmonell
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Rolle, Kenya, Margaret W. Gitau, Gang Chen, and Ashvini Chauhan. "Assessing fecal coliform fate and transport in a coastal watershed using HSPF." Water Science and Technology 66, no. 5 (2012): 1096–102. http://dx.doi.org/10.2166/wst.2012.282.

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There is increasing concern regarding spread and proliferation of pathogenic microorganisms in watersheds and their impacts on water quality. In agricultural and rural watersheds fecal coliform occurrence, an indicator of pathogenic contamination, is often thought to be a result of land applications of animal waste. This study used the Hydrologic Simulation Program Fortran (HSPF) along with the Bacterial Indicator Tool to model fecal coliform transport in a coastal watershed with a view to identifying contaminant sources and key contributing areas. Results indicated that the highest levels of
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23

Borrusso, Patricia A., and Jennifer J. Quinlan. "Prevalence of Pathogens and Indicator Organisms in Home Kitchens and Correlation with Unsafe Food Handling Practices and Conditions." Journal of Food Protection 80, no. 4 (2017): 590–97. http://dx.doi.org/10.4315/0362-028x.jfp-16-354.

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ABSTRACT Despite education efforts, consumers often practice unsafe food handling and storage behaviors. Little is known about how these unsafe practices contribute to contamination of the home kitchen with foodborne pathogens. In addition, only a limited number of studies have examined the role of the kitchen as a reservoir for pathogens. The purpose of this study was to characterize microbial contamination and foodborne pathogens found in home kitchens and determine whether contamination was significantly associated with unsafe or unsanitary conditions observed in the kitchen. Swab samples w
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24

Wade, Timothy J., Benjamin F. Arnold, Ken Schiff, et al. "Health risks to children from exposure to fecally-contaminated recreational water." PLOS ONE 17, no. 4 (2022): e0266749. http://dx.doi.org/10.1371/journal.pone.0266749.

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Background Children may be at higher risk for swimming-associated illness following exposure to fecally-contaminated recreational waters. We analyzed a pooled data set of over 80,000 beachgoers from 13 beach sites across the United States to compare risks associated with the fecal indicator bacteria Enterococcus spp. (measured by colony forming units, CFU and quantitative polymerase chain reaction cell equivalents, qPCR CE) for different age groups across different exposures, sites and health endpoints. Methods Sites were categorized according to the predominant type of fecal contamination (hu
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Lévesque, Benoît, Fabien Gagnon, Alexandra Valentin, et al. "Étude de la contamination microbienne des myes (Mya arenaria) de la rive nord de l'estuaire maritime du fleuve Saint-Laurent (Québec, Canada)." Canadian Journal of Microbiology 52, no. 10 (2006): 984–91. http://dx.doi.org/10.1139/w06-061.

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The aims of the present study were to assess the microbial quality of Mya arenaria clams from the north shore of the St. Lawrence River estuary and to validate various microbial indicator microorganisms of bivalve mollusks contamination. Clams were collected from nine sites, including four harvesting sites closed by virtue of the Canadian Shellfish Sanitation Program (CSSP). Six contamination indicators (fecal coliforms, somatic coliphages, F-specific coliphages, fecal streptococci, Clostridium perfringens, and Escherichia coli) and four pathogens (Campylobacter sp., Cryptosporidium parvum, Gi
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26

Field, R., M. O'Shea, and M. P. Brown. "The Detection and Disinfection of Pathogens in Storm-Generated Flows." Water Science and Technology 28, no. 3-5 (1993): 311–15. http://dx.doi.org/10.2166/wst.1993.0432.

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A recreational water's disease-producing potential is usually assessed by bacterial indicators of human fecal contamination, however many of these indicator bacteria also originate from soils, vegetation, and animal feces. Stormwater runoff can contain high densities of the nonhuman indicator bacteria and epidemiological studies of recreational waters receiving stormwater runoff have found little correlation between indicator densities and swimming related illnesses. In addition a number of non-enteric pathogens found in stormwater runoff have been linked to respiratory illnesses and skin infe
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27

Linden, R., MV Antunes, LS Heinzelmann, et al. "Caffeine as an indicator of human fecal contamination in the Sinos River: a preliminary study." Brazilian Journal of Biology 75, no. 2 suppl (2015): 81–84. http://dx.doi.org/10.1590/1519-6984.0513.

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<p>The preservation of hydric resources is directly related to fecal contamination monitoring, in order to allow the development of strategies for the management of polluting sources. In the present study, twenty-five water samples from six water public supply collection sites were used for the evaluation of the presence of caffeine, total and fecal coliforms. Caffeine was detected in all samples, with concentrations ranging from 0.15 ng mL<sup>–1</sup> to 16.72 ng mL<sup>–1</sup>. Total coliforms were detected in all samples, with concentrations in the range of 5
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Stelma, Gerard N., and Larry J. Wymer. "Research considerations for more effective groundwater monitoring." Journal of Water and Health 10, no. 4 (2012): 511–21. http://dx.doi.org/10.2166/wh.2012.016.

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Since numerous pathogens occur in feces, water is monitored for fecal contamination using indicator organisms rather than individual pathogens. Although this approach is supported by health effects data in recreational waters, it is questionable when used for drinking water. Most outbreaks in groundwater occur in systems that have not violated the US EPA's maximum contaminant limit (MCL) for total coliforms within 12 months before the outbreak. Additionally, environmentally stable viruses and parasites are often detected in drinking water samples with no detectable indicators. Recent detection
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Alrazig, Hala Krim Eldin Abd, Ahmed Mohammed Ahmed, Leila M. Ahmed Abdelgader, et al. "Isolation and Identification of Bacterial Contamination Water Supply in Shendi City-Sudan." Middle East Research Journal Microbiology and Biotechnology 4, no. 01 (2024): 6–9. http://dx.doi.org/10.36348/merjmb.2024.v04i01.002.

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Background: Microbial contamination of water persists to be a significant problem; the usual source is human and animal fecal matter that has contaminated the water systems. The presence of bacteria and pathogenic organisms is of great concern when considering the safety of drinking water, as pathogenic organisms can cause watery diarrhea, cholera, typhoid fever, and other illnesses. Objective: This is a prospective tap water examination study to find groundwater microbiological fecal contamination. Materials and Methods: Water samples for laboratory examination were collected from a different
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Bofill-Mas, Sílvia, Marta Rusiñol, Xavier Fernandez-Cassi, Anna Carratalà, Ayalkibet Hundesa, and Rosina Girones. "Quantification of Human and Animal Viruses to Differentiate the Origin of the Fecal Contamination Present in Environmental Samples." BioMed Research International 2013 (2013): 1–11. http://dx.doi.org/10.1155/2013/192089.

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Many different viruses are excreted by humans and animals and are frequently detected in fecal contaminated waters causing public health concerns. Classical bacterial indicator such asE. coliand enterococci could fail to predict the risk for waterborne pathogens such as viruses. Moreover, the presence and levels of bacterial indicators do not always correlate with the presence and concentration of viruses, especially when these indicators are present in low concentrations. Our research group has proposed new viral indicators and methodologies for determining the presence of fecal pollution in
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Eifan, Saleh, Khalid Maniah, Islam Nour, et al. "Pepper Mild Mottle Virus as a Potential Indicator of Fecal Contamination in Influents of Wastewater Treatment Plants in Riyadh, Saudi Arabia." Microorganisms 11, no. 4 (2023): 1038. http://dx.doi.org/10.3390/microorganisms11041038.

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Several indicators of fecal pollution in water resources are continuously monitored for their reliability and, of particular interest, their correlation to human enteric viruses—not justified by traditional bacterial indicators. Pepper mild mottle virus (PMMoV) has recently been proposed as a successful viral surrogate of human waterborne viruses; however, in Saudi Arabia there are no available data in terms of its prevalence and concentration in water bodies. The concentration of PMMoV in three different wastewater treatment plants (King Saud University (KSU), Manfoha (MN), and Embassy (EMB)
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Ahmed, W., R. Neller, and M. Katouli. "Population similarity of enterococci and Escherichia coli in surface waters: A predictive tool to trace the sources of fecal contamination." Journal of Water and Health 4, no. 3 (2006): 347–56. http://dx.doi.org/10.2166/wh.2006.042.

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A biochemical fingerprinting method (the PhPlate system) was used to compare similarities between Escherichia coli and enterococci populations from surface water samples with those found in different animal species during the wet and the dry seasons in order to predict the dominant source(s) of fecal contamination in a local creek. A significant increase in the number and diversity of enterococci was observed in the creek during the wet season. Enterococci population from water samples also showed a higher population similarity with animal species than did E. coli. A higher population similari
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FLISS, I., R. E. SIMARD, and A. ETTRIKI. "Microbiological Quality of Different Fresh Meat Species in Tunisian Slaughterhouses and Markets." Journal of Food Protection 54, no. 10 (1991): 773–77. http://dx.doi.org/10.4315/0362-028x-54.10.773.

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This study was undertaken to evaluate the bacterial contamination level of fresh meat from four different (meat samples) animal species, namely bovine, ovine, poultry, and equine (270 carcasses) collected in various Tunisian slaughterhouses and markets. The level of contamination was assessed using the excised skin technique. All meat surface samples were analyzed for total aerobic microflora, total coliforms, fecal coliforms, and Escherichia coli as an indicator of fecal contamination. Regardless of animal species considered, counts were relatively high for fresh prepared meat. The mean level
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34

Savichtcheva, O., and S. Okabe. "Qualitative and quantitative estimation of host-specific fecal pollution using Bacteroides–Prevotella 16S rRNA genetic markers by T-RFLP and real-time PCR analyses." Water Science and Technology 59, no. 9 (2009): 1831–40. http://dx.doi.org/10.2166/wst.2009.213.

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Rapid and reliable determination of the non-point sources of fecal pollution is a critical issue for the environmental microbiologists all over the world. In this work we evaluated the use of anaerobic bacterial group Bacteroides–Prevotella as an alternative fecal pollution indicator. Terminal restriction fragment length polymorphism (T-RFLP) and real-time polymerase chain reaction (RT-PCR) analyses were used to monitor and quantify human-, cow- and pig-specific fecal contamination in natural river waters. We also included DNA sequence analysis of the identified fecal markers revealed by T-RFL
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Green, Hyatt, Daniel Weller, Stephanie Johnson, and Edward Michalenko. "Microbial Source-Tracking Reveals Origins of Fecal Contamination in a Recovering Watershed." Water 11, no. 10 (2019): 2162. http://dx.doi.org/10.3390/w11102162.

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Fecal contamination of waterbodies due to poorly managed human and animal waste is a pervasive problem that can be particularly costly to address, especially if mitigation strategies are ineffective at sufficiently reducing the level of contamination. Identifying the most worrisome sources of contamination is particularly difficult in periurban streams with multiple land uses and requires the distinction of municipal, agricultural, domestic pet, and natural (i.e., wildlife) wastes. Microbial source-tracking (MST) methods that target host-specific members of the bacterial order Bacteroidales an
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Malla, Bikash, Rajani Ghaju Shrestha, Sarmila Tandukar, et al. "Identification of Human and Animal Fecal Contamination in Drinking Water Sources in the Kathmandu Valley, Nepal, Using Host-Associated Bacteroidales Quantitative PCR Assays." Water 10, no. 12 (2018): 1796. http://dx.doi.org/10.3390/w10121796.

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This study identified the sources of fecal contamination in the groundwater of different land covers. A total of 300 groundwater samples were collected in the Kathmandu Valley, Nepal, in the dry (n = 152) and wet (n = 148) seasons of 2016. Fecal indicator bacteria were initially enumerated, and then fecal contamination sources were identified using human (BacHum), ruminant (BacR), and pig-associated (Pig2Bac) Bacteroidales quantitative polymerase chain reaction assays. Sixty-six percent (197/300) of the tested groundwater samples had Escherichia coli concentrations higher than the World Health
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Noble, Rachel T., John F. Griffith, A. Denene Blackwood, et al. "Multitiered Approach Using Quantitative PCR To Track Sources of Fecal Pollution Affecting Santa Monica Bay, California." Applied and Environmental Microbiology 72, no. 2 (2006): 1604–12. http://dx.doi.org/10.1128/aem.72.2.1604-1612.2006.

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ABSTRACT The ubiquity of fecal indicator bacteria such as Escherichia coli and Enterococcus spp. in urban environments makes tracking of fecal contamination extremely challenging. A multitiered approach was used to assess sources of fecal pollution in Ballona Creek, an urban watershed that drains to the Santa Monica Bay (SMB) near Los Angeles, Calif. A mass-based design at six main-stem sites and four major tributaries over a 6-h period was used (i) to assess the flux of Enterococcus spp. and E. coli by using culture-based methods (tier 1); (ii) to assess levels of Enterococcus spp. by using q
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38

Zhang, Qian, Xia He, and Tao Yan. "Impact of indigenous microbiota of subtidal sand on fecal indicator bacteria decay in beach systems: a microcosm study." Environmental Science: Water Research & Technology 1, no. 3 (2015): 306–15. http://dx.doi.org/10.1039/c5ew00004a.

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Mazhar, Sumaira, Adeeba Syed, Bushra Nisar Khan, and Roheela Yasmin. "Fecal Matter as a Bio-indicator of Heavy Metal Toxification in Punjab Urial." Lahore Garrison University Journal of Life Sciences 1, no. 2 (2020): 99–103. http://dx.doi.org/10.54692/lgujls.2017.010285.

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Heavy metals are a major class of pollutants that are responsible for high level of toxicity in living beings. These metals have the tendency to bio-accumulate in the living tissues; their levels can be determined in the various organs of the body. Most of the old methods used for the indication of heavy metal contamination in the environment usually involve the killing of animals, whereas current study is used to determine the heavy metal contamination in fecal matter, feed, water and soil without causing any harm to the lives of animals. Observed level of heavy metal like cadmium (0.0073 ppm
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Srijew, Supattra, Wilai Chiemchaisri, Chart Chiemchaisri, and Hiroyasu Satoh. "Source identification of fecal contamination in the canals in Bangkok using fecal sterol compounds." Journal of Water, Sanitation and Hygiene for Development 6, no. 1 (2016): 13–22. http://dx.doi.org/10.2166/washdev.2016.033.

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This study aims to apply fecal sterols as a chemical indicator to track feces contamination sources in the canals in Bangkok. The sewage and canal sediments were examined during the dry season. Fecal sterols in the canals were evaluated over a one-year period (2011–2012) for the selected sterol compounds. Several factors including season, canal water levels, rainfall data, and sewage were correlated to fecal contamination. The highest concentrations of cholesterol (CHR) (8.321 μg/L), coprostanol (CPN) (6.490 μg/L), and cholestanol (CHN) (1.205 μg/L) in discharged sewage were found from the com
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Krentz, Corinne A., Natalie Prystajecky, and Judith Isaac-Renton. "Identification of fecal contamination sources in water using host-associated markers." Canadian Journal of Microbiology 59, no. 3 (2013): 210–20. http://dx.doi.org/10.1139/cjm-2012-0618.

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In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water samples for multiple fecal contamination markers simultaneously are currently lacking. The objectives of this study were (i) to develop a qualitative MST tool to identify fecal contamination from different host groups, and (ii) to evaluate the MST tool using water samples with evidence
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Burnet, Jean-Baptiste, Marc Habash, Mounia Hachad, et al. "Automated Targeted Sampling of Waterborne Pathogens and Microbial Source Tracking Markers Using Near-Real Time Monitoring of Microbiological Water Quality." Water 13, no. 15 (2021): 2069. http://dx.doi.org/10.3390/w13152069.

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Waterborne pathogens are heterogeneously distributed across various spatiotemporal scales in water resources, and representative sampling is therefore crucial for accurate risk assessment. Since regulatory monitoring of microbiological water quality is usually conducted at fixed time intervals, it can miss short-term fecal contamination episodes and underestimate underlying microbial risks. In the present paper, we developed a new automated sampling methodology based on near real-time measurement of a biochemical indicator of fecal pollution. Online monitoring of β-D-glucuronidase (GLUC) activ
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Harwood, Valerie J., John Whitlock, and Victoria Withington. "Classification of Antibiotic Resistance Patterns of Indicator Bacteria by Discriminant Analysis: Use in Predicting the Source of Fecal Contamination in Subtropical Waters." Applied and Environmental Microbiology 66, no. 9 (2000): 3698–704. http://dx.doi.org/10.1128/aem.66.9.3698-3704.2000.

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ABSTRACT The antibiotic resistance patterns of fecal streptococci and fecal coliforms isolated from domestic wastewater and animal feces were determined using a battery of antibiotics (amoxicillin, ampicillin, cephalothin, chlortetracycline, oxytetracycline, tetracycline, erythromycin, streptomycin, and vancomycin) at four concentrations each. The sources of animal feces included wild birds, cattle, chickens, dogs, pigs, and raccoons. Antibiotic resistance patterns of fecal streptococci and fecal coliforms from known sources were grouped into two separate databases, and discriminant analysis o
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Bernhard, Anne E., and Katharine G. Field. "Identification of Nonpoint Sources of Fecal Pollution in Coastal Waters by Using Host-Specific 16S Ribosomal DNA Genetic Markers from Fecal Anaerobes." Applied and Environmental Microbiology 66, no. 4 (2000): 1587–94. http://dx.doi.org/10.1128/aem.66.4.1587-1594.2000.

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ABSTRACT We describe a new PCR-based method for distinguishing human and cow fecal contamination in coastal waters without culturing indicator organisms, and we show that the method can be used to track bacterial marker sequences in complex environments. We identified two human-specific genetic markers and five cow-specific genetic markers in fecal samples by amplifying 16S ribosomal DNA (rDNA) fragments from members of the genus Bifidobacterium and theBacteroides-Prevotella group and performing length heterogeneity PCR and terminal restriction fragment length polymorphism analyses. Host-speci
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Pizzi, Nick, and Mary Roland. "Coliform Tests: An Evolution Serving Public Health." Opflow 50, no. 9 (2024): 8–9. http://dx.doi.org/10.1002/opfl.2018.

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It's difficult and expensive to routinely examine waters for the presence of every type of pathogen. It's more practical to screen water for the presence of fecal contamination by testing for the presence of an “indicator” microorganism.
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Paytan, Adina, Alexandria B. Boehm, and Gregory G. Shellenbarger. "Bacterial Contamination and Submarine Groundwater Discharge—A Possible Link." Environmental Chemistry 1, no. 1 (2004): 29. http://dx.doi.org/10.1071/en04002.

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Environmental Context.Pathogenic bacteria and viruses are sometimes detected in coastal waters, high levels of which correlate with occurrence of acute gastrointestinal illness in recreational beach users. The source of the bacterial and viral contamination to the beach is not always easy to decipher, and therefore efforts to prevent these occurrences are limited by lack of full understanding of their cause. Abstract.Beach water contamination by pathogenic agents is monitored by Fecal Indicator Bacteria (FIB) levels. A source of these agents may be submarine groundwater discharge. At Huntingto
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Hathaway, J. M., W. F. Hunt, R. M. Guest, and D. T. McCarthy. "Residual indicator bacteria in autosampler tubing: a field and laboratory assessment." Water Science and Technology 69, no. 5 (2014): 1120–26. http://dx.doi.org/10.2166/wst.2014.035.

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Microbial contamination in surface waters has become a worldwide cause for concern. As efforts are made to reduce this contamination, monitoring is integral to documenting and evaluating water quality improvements. Autosamplers are beneficial in such monitoring efforts, as large data sets can be generated with minimized effort. The extent to which autosamplers can be utilized for microbial monitoring is largely unknown due to concerns over contamination. Strict sterilization regimes for components contacting the water being sampled are difficult, and sometimes logistically implausible, when ut
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ENDLEY, SEEMA, LINGENG LU, EVERARDO VEGA, MICHAEL E. HUME, and SURESH D. PILLAI. "Male-Specific Coliphages as an Additional Fecal Contamination Indicator for Screening Fresh Carrots." Journal of Food Protection 66, no. 1 (2003): 88–93. http://dx.doi.org/10.4315/0362-028x-66.1.88.

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The objective of this study was to evaluate the efficacy of male-specific (F+) coliphages as a fecal-contaminationindicator for fresh carrots. The prevalence of specific pathogens and indicator organisms on the surface of carrots obtained from a farm, truck, and processing shed was studied. Twenty-five carrot samples collected from each of these locations were washed, and aliquots of the wash were analyzed for the presence of F+ coliphages, Escherichia coli, Salmonella, and Shigella. Additionally, the Salmonella isolates were genotyped using pulsed-field gel electrophoresis (PFGE). Our studies
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EL-HACHEMI, Mohammed Fayçal, Merzouk Yamina, Gourari Imene, Hachemi Kaouter, and Ayat Mohamed. "Physico-chemical and bacteriological qualitative study of Mediterranean coastal swimming seas." South Asian Journal of Experimental Biology 12, no. 2 (2022): 245–53. http://dx.doi.org/10.38150/sajeb.12(2).p245-253.

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The beaches of Oran are among the busiest in Algeria. However these beaches are exposed to multiple dangers in particular to pollution which poses serious problems and which can also hinder any proper use of the sea.This paper is an assessment of the physico-chemical and bacteriological quality of some beaches where swimming is allowed along the Oranese coast in Algeria, specifically the Kristel Beach and the beach of Les Andalouses seaside resort. Fecal streptococci are normal hosts of the intestine of humans and warm-blooded animals. Research concerning them associated with that of fecal col
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Korajkic, Asja, Brian McMinn, and Valerie Harwood. "Relationships between Microbial Indicators and Pathogens in Recreational Water Settings." International Journal of Environmental Research and Public Health 15, no. 12 (2018): 2842. http://dx.doi.org/10.3390/ijerph15122842.

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Fecal pollution of recreational waters can cause scenic blight and pose a threat to public health, resulting in beach advisories and closures. Fecal indicator bacteria (total and fecal coliforms, Escherichia coli, and enterococci), and alternative indicators of fecal pollution (Clostridium perfringens and bacteriophages) are routinely used in the assessment of sanitary quality of recreational waters. However, fecal indicator bacteria (FIB), and alternative indicators are found in the gastrointestinal tract of humans, and many other animals and therefore are considered general indicators of fec
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