Journal articles on the topic 'Feng shen yen i'

A new unambiguous tracking technique based on combined correlation functions for sine Binary Offset Carrier (BOC) signals is proposed in this paper. The key to this method is to exploit two types of local reference signals: the BOC signal and a linear combination of a series of BOC signals with different delays. They are both correlated with the received signals. Then, a correlation function without any positive side peaks is obtained by multiplying the two correlation results to make tracking completely unambiguous. Theoretical analysis and simulation in the tracking stage show that the proposed method has the best code tracking accuracy among the method tracking BOC signals like Binary Phase-Shift Keying signals (BPSK-LIKE), the Pseudo correlation function based Unambiguous Delay Lock Loop (PUDLL), Symmetrical Pulse Ambiguity Removing (SPAR) technique, the method proposed by Shen Feng (SF) and the two methods proposed by Yan Tao (YT-V1 and YT-V2). In multipath environments, the proposed method has the best anti-multipath performance of all the tracking methods mentioned above. In conclusion, the proposed method can completely eliminate ambiguity and has significant performance advantages compared with the methods mentioned above.

En este artículo se presenta una versión condicionada de la generalización del Lema de Borel-Cantelli debida a Feng, Li y Shen. Este resultado mejora el Lema de Borel-Cantelli, en tanto que implica otras versiones condicionadas que aparecen en la literatura. Para demostrar el resultado principal se da una versión condicionada de la Desigualdad de Chung-Erdös.

Background. This study aimed to investigate the distribution and characteristics of traditional Chinese medicine (TCM) syndrome and its elements on respiratory diseases (RDs) based on real-world data (RWD). Methods. A real-world study was performed to explore the relationships among TCM syndrome and RDs based on electronic medical information. A total of 26,074 medical records with complete data were available for data analysis. Factor analyses were used to reduce dimensions of TCM syndrome elements and detect common factors. Additionally, cluster analyses were employed to assess combinations of TCM syndrome elements. Finally, association rule analyses were performed to investigate the structures of TCM syndrome elements to estimate the patterns of TCM syndrome. Results. A total of 27 TCM syndromes were extracted from RWD in this work. There were four TCM syndromes with >5.0% frequency based on the distribution frequency. The top five pathogenesis TCM syndrome elements were Tan, Huo, Feng, Qi_Xu, and Han. Factor analysis, cluster analysis, and association rule analysis demonstrated that Tan, Huo, Feng, Qi_Xu, Shen, and Fei were the core TCM syndrome elements. Conclusion. Four common Shi TCM syndromes on RDs were identified: Tan_Re_Yong_Fei, Tan_Zhuo_Zu_Fei, Feng_Re_Fan_Fei, and Feng_Han_Xi_Fei; two core common Xu TCM syndromes (Fei_Shen_Qi_Xu and Fei_Yin_Xu) and two core common Mix TCM syndromes (Fei_Pi_Qi_Xu-Tan_Shi_Yun_Fei and Fei_Shen_Qi_Xu-Tan_Yu_Zu_Fei) were also determined. The core TCM syndrome elements of Tan, Huo, Feng, Qi_Xu, Shen, and Fei were identified in this work.

Up to this point I have attempted to show how Shen Nung was portrayed in late Warring States and early Han texts. I have also reviewed what we know about Nung (or Chu) and Lieh Shan shih, Keng-fu (Plough Father), and T'ien-tsu (Field Ancestor), since it may be one or all four of these figures that comes to be known as Shen Nung.We can argue nothing for certain on this, and certainly T'ien-tsu in the Shih, was, we assume, a god of the Chou. But that point aside, there seems to be good reason to think that Shen Nung was originally a farming god of the Shang people. He is the hero who invented the plough, and the god to whom they turned when they needed rain. Ultimately, this may have been Hsieh, the first-born of the Shang people, known to us in our texts as Shu-chün or Yi-chün. That Shen Nung would become prominent only in texts that date from the late Chou should be no surprise; we should expect him to be overshadowed in early Chou times by their hero Hou Chi.It is important to keep in mind as we proceed that in the materials we have examined so far, we have seen repeated references to ‘fire’ and ‘rain’ and ‘drought’. Ritual ways of producing rainfall and combating the drought could involve fire (yen-huo, in Shih, 211); they might also involve ‘invocations’, charging the drought demon to ‘Go to the north!’ (Shan-hai ching).

Objective: To investigate the distribution and characteristics of traditional Chinese medicine (TCM) syndromes and its elements on chronic bronchitis (CB) based on real-world data (RWD) so as to optimize the treatment strategies. Methods: A real-world study based on 2207 medical records collected from five hospitals in China, to explore the relationship between TCM syndrome and CB using the big data methods. Factor analyses were used to reduce the dimensions of TCM syndrome elements and found common factors. Additionally, cluster analyses were performed to value combinations of TCM syndrome element. Finally, association rule analyses were employed to assess the structures of TCM syndromes elements and estimate the patterns of TCM syndrome. Results: A total of 21 TCM syndromes were extracted from RWD in this work. There were four TCM syndromes consisting of Tan_Zhuo_Zu_Fei, Tan_Re_Yong_Fei, Feng_Han_Xi_Fei, and Feng_Re_Fan_Fei with [Formula: see text]% frequency based on the distribution frequency. The two top Xu TCM syndromes of Fei_Yin_Xu and Fei_Shen_Qi_Xu were identified. The top six pathogenesis TCM syndrome elements were Tan, Huo, Feng, Han, Qi_Xu, and Yin_Xu. Factor analyses, cluster analyses, and association rule analyses demonstrated that Tan, Huo, Feng, Han, Qi-Xu, Yin-Xu, Fei, and Shen were the core TCM syndrome elements. Conclusion: The four common Shi TCM syndromes of Tan_Zhuo_Zu_Fei, Tan_Re_Yong_Fei, Feng_Han_Xi_Fei, and Feng_Re_Fan_Fei for CB were detected in the real world study, and the two Xu TCM syndromes of Fei_Yin_Xu and Fei_Shen_Qi_Xu were identified. The Mix TCM syndrome of Fei_Pi_Qi_Xu_Tan_Shi_Yun_Fei was the main syndrome. The core TCM syndrome elements of Tan, Huo, Feng, Han, Qi_Xu, and Yin_Xu, Fei, and Shen were determined in the entire sample.

Musty and earthy odor are present in the source water of Feng-Shen waterworks (FSW) in south Taiwan all year round. Although 2-methylisoborneol (2-MIB) and geosmin are responsible for the musty and earthy odor, respectively, the possible odor producers remained unknown. In this study, actinomycetes that produce 2-MIB and geosmin were studied. Based on the 16S rDNA sequence, three species were identified after comparison with the GenBank database at the NCBI. The three species are Streptomyces malaysiensis as a 2-MIB and geosmin producer, Streptomyces caelestis as a 2-MIB producer, and Streptomyces roseoflavus as a geosmin producer. For S. malaysiensis and S. caelestis, the production of MIB and/or geosmin was increased with incubation temperature (20–30 °C) in starch–glutamate broth medium. The optimal MIB, geosmin and biomass occurred at 30 °C. The maximal geosmin/biomass (G/B) and/or MIB/biomass (M/B) also occurred at 30 °C, and sporulating cultures contained more geosmin and/or MIB than nonsporulatig cultures.

While modeling our late time cosmically accelerated universe, it is popular to involve different dark energy (DE) models, the equation of state (EoS) of which can be taken as a function of the redshift and some unknown parameters. Barboza and Alcaniz have proposed one such kind of DE EoS model. We use some new parametrizations like Feng, Shen, Li, Li I and II and Polynomial parametrizations to get more accurate concepts about the fate of our expanding universe. We try to find how the hypothesis of the fate of our universe behaves in the above background of DE models. Possibilities of occurrences of future cosmic singularities are studied.

The Chin-so liu-chu yin is a text of twenty-nine fascicles preserved in the Taoist canon in the form of a revelation to Chang Tao-ling, the late Han founder of the Taoist religion, to which comments by Li Ch‘un-feng (602–670) are attached. Though scholars have not so far addressed the question of the origins of the text itself, a certain willingness to accept the attribution of the commentary has already been made manifest. Yet to the eye of the expert in Sung Taoism this attribution raises serious doubts: text and commentary display features much more reminiscent of Sung religion than that of the early T'ang. It has already been noticed that Li Ch'un-feng provides information on the cult of the city gods (ch'eng-huang shen) and on Thunder magic; the former religious development may be traced back before the T‘ang but only achieved prominence towards the end of the dynasty, while the latter is unattested in T‘ang Taoist literature. One might add that Li displays a detailed knowledge of the legend of Hsü Sun in a developed form—again a cult v with early origins hardly noticed until the late T‘ang. The text also provides information (though separately) on the Buddhist warrior-king Vaisravana (P‘isha-men PI) and on the seventh-century warrior-hero Li Ching (571–649): these two figures were eventually to merge.

This article examines the historical conditions, causes, evolution and options for solving one of the topical problems of the spatial historical dynamics of China in the late XX - early XXI centuries. - uneven regional development. The purpose of this article is to review in Chinese the works of such Chinese scholars as Wang Ke, Wang Feng, Wang Yunhui, Gan Chunhui, Li Zhuni, Liang Longbin, Xia Wanjun, Fan Jianyong, Hu Dali, Hu Shudong, Tsai Fang, Zhou Zhe, Zhu Degui, Chen Rong, Chen Changshi, Shi Lin, Shen Zhongqiang on the history of the uneven regional development of the PRC and highlighting the leading approaches and practical recommendations for overcoming disproportionate trends in the regional historical and modern development of China based on the use of system analysis and a comparative historical method. The main factors that significantly affect the uneven regional development of China in the modern period of history include: uneven industrial development, income distribution by provinces, foreign investment, agriculture, human capital and labor mobility.

This study presents a methodology for improving the efficiency of Baptist and Stone and Shen models in predicting the global water flow resistance of a reclamation channel partly vegetated by rigid and emergent riparian plants. The results of the two resistance models are compared with the measurements collected during an experimental campaign conducted in a reclamation channel colonized by Common reed (Phragmites australis (Cav.) Trin. ex Steud.). Experimental vegetative Chézy’s flow resistance coefficients have been retrieved from the analysis of instantaneous flow velocity measurements, acquired by means of a downlooking 3-component acoustic Doppler velocimeter (ADV) located at the channel upstream cross section, and by water level measurements obtained through four piezometers distributed along the reclamation channel. The main morphometrical vegetation features (i.e., stem diameters and heights, and bed surface density) have been measured at six cross sections of the vegetated reclamation channel. Following the theoretical assumptions of the divided channel method (DCM), three sub-sections have been delineated in the reference cross section to represent the impact of the partial vegetation cover on the cross sectional variability of the flow field, as observed with the ADV measurements. The global vegetative Chézy’s flow resistance coefficients have been then computed by combining each resistance model with four different composite cross section methods, respectively suggested by Colebatch, Horton, Pavlovskii, and Yen. The comparative analysis between the modeled and the experimental vegetative Chézy’s coefficients has been performed by computing the relative prediction error (εr, expressed in %) under two flow rate regimes. Stone and Shen model combined with the Horton composite cross section method provides vegetative Chézy’s coefficients with the lowest εr.

In the late Ming dynasty, a new genre of drama arose, which presented on stage recent political events, featuring real historical persons; this genre continued across the Ming-Qing transition. The earliest and one of the best known examples is The Cry of the Phoenix (Ming feng ji), dramatising the conflict between corrupt minister Yan Song (1481-1568) and upright official Yang Jisheng (1516-1555), and probably written by someone in the literary circle of Wang Shizhen (1526-1590). The genre reached its apogee in Kong Shangren’s (1648-1718) The Peach Blossom Fan (Taohua shan). Around the Ming-Qing transition, in the Chongzhen and Shunzhi reigns, a considerable number of plays focused on the conflict during the preceding Tianqi reign between the Eastern Grove (Donglin) faction and the chief eunuch Wei Zhongxian (1568-1627). Eleven plays on this subject are known, of which three survive: Fan Shiyan’s Eunuch Wei Grinds Down the Loyal (Wei jian mo zhong ji), the Clear-Whistling Scholar’s (Qingxiaosheng) A Happy Encounter with Spring (Xi feng chun), and Li Yu’s 李玉 A Roster of the Pure and Loyal (Qing zhong pu). Basing my argument on an examination of these plays and of another play by Li Yu, Reunion across Ten Thousand Miles (Wan li yuan), also based on contemporary events, I suggest that the lively version of events given by these political dramas both reflected and helped to develop and spread the popularly accepted view of late-Ming and Southern Ming factional conflict leading to the fall of the Ming dynasty. According to this view, broadly following the Eastern Grove and Revival Society (Fushe) narrative, the decline and fall of the Ming dynasty was the fault of corrupt officials and evil palace eunuchs who misled the Emperor and were bravely resisted by righteous and incorruptible officials who fell as martyrs to their unprincipled opponents. This simplistic view, endorsed to a great extent in the official Ming History (Ming shi), which was mostly written by former Eastern Grove and Revival Society adherents, has persisted in the popular mind to the present day. I also argue that, after the establishment of the Qing, political drama could serve as a vehicle for the covert expression of Ming loyalism.

The theory of Emotion Coaching was put forward by Feng Menglong in Late-Ming Dynasty. Since Mid-Ming, the cognition of Ch’ing (emotion) has gone through three phases: rediscovery, development, and explanation on the level of the mind. Deeply influenced by Yangming, the theory of Emotion Coaching focuses neither on Ch’ing (emotion) nor on Li (justice) but the balance of the two. That “Ch’ing (emotion) is the basis of Li (justice) and Li (justice) is the criterion of Ch’ing (emotion)” is taken as the tenet of the theory, which means that Ch’ing (emotion) gives rise to moral behaviour and Li (justice) is the standard. The book The History of Ch’ing (《情史》) fully reflects the theory. Separating emotion and desire, Li (justice) is different from Li (rite). The former is based on human nature. Justice and destiny are the two important principles, rather than the political purposes which infuse the latter. The theory of Emotion Coaching is also reflected in the collection of short novels San Yen (“三言”). In the novel, the details of emotion are fantastic, trying to make sense of emotional varieties. Affective and wanton behaviour are shown differently in order to illustrate the discrepancy between emotion and desire. Justice and destiny are also emphasized. Chiang Hsingge Regained His Pearl Shirt (《蒋兴 哥重会珍珠衫) serves as an example here.

As a reviewer, John Brown received the first Lepidoptera manuscript submitted to Zootaxa in 2002. Within a year he was persuaded by a colleague to volunteer as its first Lepidoptera section editor. As submissions increased, he realized that he needed assistance, so in July 2005 he enlisted Robert Robbins (U.S. National Museum of National History), and the two editors split the submissions—Brown covered moths and Robbins butterflies. As submissions continued to grow, Robbins stepped down and Brown was again the sole editor. Owing to the ever-increasing manuscript load, in 2007 Brown submitted a proposal to several colleagues, inviting them to become Lepidoptera section editors, with the concept that the more editors there were, the fewer manuscripts each would have to handle, and their duties would include papers primarily in their area of expertise. The solicitation was successful, with four new subject editors coming on board in 2007: Lawrence Gall for macrolepidoptera families, Michael Toliver for butterflies, Jean-François Landry for microlepidoptera families, and Shen-Horn Yen for Pyraloidea and Zygaenoidea; the last two are still section editors today. Over the next 13 years, numerous editors came and went—turnover in editorship was always viewed as a positive way to involve new scientists and interject fresh ideas. From 2001 to 2020, a cumulative total of 21 scientists have served as Lepidoptera Section editors (Table 1), representing 14 different countries.

In this study, the effect of three oxidants, sodium hypochlorite, potassium permanganate, and ozone, were tested for the removal of 2-MIB with presence of cyanobacteria. Algae in water samples from the source water of Feng-Shen waterworks (FSW), Taiwan were cultivated at 30¡C with continuous light at an intensity between 2,500 and 3,400 lux. During the cultivating process, water samples were analyzed for nutrients, light absorbance at 665nm (A665), and 2-MIB concentration. The 2-MIB concentrations within the incubated samples increased to as high as 1,000 ng/L to 2,000 ng/L, although no extra nutrients were added to the raw water. After 2 to 3 days incubation, the intracellular 2-MIB concentration was as high as 70% of the total 2-MIB in the samples. The algae that developed were mainly cyanobateria, and more than 90% belonged to the Genus Oscillatorias. An almost 100% removal of both 2-MIB and geosmin in the raw water was observed after ozonation for 10 minutes at a dosing rate of 0.91 mg/l-min. Chlorine and permanganate were much less effective, both removing only about 11% of the 2-MIB within 60 minutes at oxidant concentration of 10 mg/l. Oxidation of the cultivated samples showed that chlorine and permanganate may damage algae cells causing them to release intracellular 2-MIB. During the 60 minutes of reaction time, the total 2-MIB concentrations (intracellular plus dissolved) varied by no more than 10%, however, the ratios between dissolved and total 2-MIB concentrations increased. Two effects of ozonation on the 2-MIB concentration in the cultivated samples were observed when the algae were young, namely 2-MIB release from damaged cells and 2-MIB oxidization. The rates of 2-MIB release and 2-MIB destruction were similar. However, old algae cells were more easily damaged. As a result, intracellular 2-MIB was released faster, and the soluble 2-MIB was destroyed more quickly by ozonation.

Целью данной работы является синтез и исследование свойств синтетического айкинита, PbCuBiS3.Синтез проводили в откачанных кварцевых ампулах в течение 7–8 ч, максимальная температура составляла 1250–1325 К. Далее образцы охлаждали и выдерживали при 600 К в течение недели. Потом ампулы вскрывали, образцы тщательно перетирали и после плавки отжигали при 600–800 К в зависимости от состава не менее двух недель для приведения образцов в равновесное состояние. Отожженные образцы исследовали методами дифференциально-термического (ДТА), рентгенофазового (РФА), микроструктурного (МСА) анализов, а также измерением микротвердости и определением плотности. РФА проводили на рентгеновском приборе модели Д 2 PHASER с использованием CuKa- излучении Ni-фильтр.Комплексом методов физико-химического анализа изучены разрезы CuBiS2–PbS, Cu2S–PbCuBiS3, Bi2S3–PbCuBiS3, PbBi2S4–PbCuBiS3, PbBi4S7–PbCuBiS3 квазитройной системы Cu2S–Bi2S3–PbS и построены их фазовые диаграммы.Установлено, что кроме сечения PbBi2S4–PbCuBiS3 все разрезы квазибинарные и характеризуются наличием ограниченных областей растворимости на основе исходных компонентов.При изучении разреза CuBiS2–PbS установлено образование четверного соединения состава PbCuBiS3, встречающееся в природе в виде минерала айкинита, плавящегося конгруэнтно при 980 К. Установлено, что соединение PbCuBiS3 кристаллизуется в ромбической сингонии с параметрами решетки: а = 1.1632, b = 1.166, с = 0.401 нм, прост. группа Pnma, Z = 4. Методами ДТА и РФА установлено, что соединение PbCuBiS3 является фазой переменного состава с областью гомогенности от 45 до 52 мол. % PbS. 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Режим доступа: https://cyberleninka.ru/article/n/nonstoichiometryin-pbcusbs3-compound24. Aliev O. M., Ajdarova D. S., Agayeva R. M., Ragimova V. M. Phaseformation in quasiternary systemCu2S–PbS–Sb2S3. Intern Journal of Application and Fundamental Research. 2016;(12): 1482–1488. Режимдоступа: https://applied-research.ru/pdf/2016/2016_12_8.pdf25. Алиев О. М., Аждарова Д. С., Агаева Р. М., Максудова Т. Ф. Фазообразование на разрезахCu2S(Sb2S3, PbSb2S4, Pb5Sb4S11)–PbCuSbS3 квазитройной системы Cu2S–Sb2S3–PbS и физические свой-ства твердых растворов (Sb2S3)1–x(PbCuSbS3)x. Неорган. материалы. 2018;54(12): 1275–1280. DOI: https://doi.org/10.1134/S002016851812001426. Рзагулуев В. А., Керимли О. Ш., Аждарова Д. С., Мамедов Ш. Г., Алиев О. М. Фазовые равновесия в системах Ag8SnS6–Cu2SnS3 и Ag2SnS3–Cu2Sn4S9. Конденсированные среды и межфазныеграницы. 2019; 21(4): 544–551. DOI: https://doi.org/10.17308/kcmf.2019.21/2365

Abstract 58 Background: In 2011, an international panel of breast cancer experts developed the first Advanced Breast Cancer (ABC) Consensus Guidelines to provide standards and improved care for the multidisciplinary care of patients with this common disease. We sought to adapt the ABC guidelines for China, incorporating cultural standards and available Chinese resource, and identifying suitable formed guideline. Methods: We organized the Chinese Consensus Guidelines Conference for ABC (CABC) yearly from 2013 through 2015 in Beijing as a joint effort between the China Medical Women's Association, the Organization of Beijing Sunshine Great Wall Oncology Program, Peking University, The panel included 50 breast oncology and surgery experts from 20 provinces, as well as two external consultant oncologists from the U.S. and Singapore. Permission was obtained from the ABC Chair to use the guidelines as a basis for our discussion. All questions were presented and discussed in detail, including a review of current applicable data, and panel members voted on each question. Results: The main issues discussed included; 1. In China the patient treatment decision making generally by family members. 2. Use of sequential single agent chemotherapy for standard risk in China most experts still prefer combination therapy. 3. The trastuzumab are not covered by health insurance in China and/or pertuzumab is not yet available. 5. For hormone receptor positive ABC, some physicians in China prefer to start with chemotherapy . 7. Not well accepted by Chinese patients. Details of final voting and Chinese consensus will be presented. Conclusions: Standard guidelines are critical, but must be tailored to be used effectively in specific countries. The CABC has effectively discussed, modified and distributed guidelines for the treatment of ABC in China. AUTHORS' DISCLOSURES OF POTENTIAL CONFLICTS OF INTEREST: Huiping Li No relationship to disclose Hope S. Rugo Honoraria: Genomic Health Speakers' Bureau: Genomic Health Research Funding: Plexxikon, Macrogenics, OBI Pharma, Eisai, Pfizer, Novartis, Eli Lilly, GlaxoSmithKline, Genentech, Celsion, Nektar, Merck, Amgen Travel, Accommodations, Expenses: Novartis, Nektar, Roche/Genentech, OBI Pharma, Mylan Jin Zhang No relationship to disclose Zhimin Shao No relationship to disclose Zhenzhou Shen No relationship to disclose Binhe Xu No relationship to disclose Jiong Wu No relationship to disclose Zefei Jiang No relationship to disclose Erwei Song No relationship to disclose Yinhua Liu No relationship to disclose Xichun Hu No relationship to disclose Cuizhi Geng No relationship to disclose Bo Li No relationship to disclose Jinhai Tang No relationship to disclose Jifeng Feng No relationship to disclose Pin Zhang No relationship to disclose Junlan Yang No relationship to disclose Qingyuan Zhang No relationship to disclose Jian Liu No relationship to disclose Yuee Teng No relationship to disclose Yongsheng Wang No relationship to disclose Zhongsheng Tong No relationship to disclose Guohong Song No relationship to disclose Peng Yuan No relationship to disclose Hongmei Zhao No relationship to disclose Wuyun Su No relationship to disclose Tao Sun No relationship to disclose Seng-Weng Wong Consulting or Advisory Role: MSD Oncology, Novartis, Roche, Pfizer Speakers' Bureau: MSD Oncology, Bayer, Novartis Travel, Accommodations, Expenses: Bayer, Roche, Merck Serono, Boehringer Ingelheim Yanshen Lu No relationship to disclose Yongchang Zhou No relationship to disclose

Abstract Abstract 3168 Background: Peripheral neuropathy (PN) is the dose-limiting toxicity of bortezomib in MM patients, affecting >30% patients, including painful PN. Treatment is mostly supportive with narcotics, antidepressants, and anticonvulsants. Acupuncture has been reported to be effective in treating neuropathic pain in diabetic and cancer patients. Methods: We initiated a pilot study to assess the feasibility, safety and efficacy of acupuncture in reducing the severity of BIPN in MM patients. Patients had acupuncture twice weekly for 2 weeks, weekly for 4 weeks, and then biweekly for 4 weeks. All patients continued their prescribed PN medications. Clinical Total Neuropathy Score (TNSc), Functional Assessment of Cancer Therapy/Gynecologic Oncology Group-Neurotoxicity (FACT/GOG-Ntx) questionnaire and Neuropathy Pain Scale (NPS) were used to assess patients at study entry and week 1, 2, 3, 4, 5, 6, 8, 10 during and after 4 weeks of completion of therapy. Serum was obtained from the patients at baseline, right after first acupuncture and before acupuncture session on week 2, 4, 8, and at follow up visit on week 14. Serum samples were used to measure cytokines concentrations. Before and after acupuncture treatments, a complete neurological examination was performed. Nerve conduction studies at baseline and end of study were conducted. Treatment included insertion of disposable sterile acupuncture needles, which retained for 25 minutes, at the following points: bilateral ear points: shen men, point zero, two auricular points where electro-dermal signal was detected, and bilateral body acupuncture points: LI4, SJ5, LI11, ST40, and Ba Feng in upper and lower extremities. Results: From May 17, 2011 to February 28, 2012, 27 MM patients were enrolled in the study. Thirteen patients were women; median age was 63 years (range: 49–77); nine were black, one Asian, 17 Caucasian, and eight had diabetes. All patients had grade 2–4 sensorimotor PN: grade 2 (n=12), grade 3 (n=14), and grade 4 (n=1); 8 had painful PN. All patients had persistant PN despite discontinuation of bortezomib for a median of 19 months (range: 1–83). Eight patients were enrolled within 6 months of stopping bortezomib, all of them had grade 3–4 painful PN. Median time from diagnosis of MM to study entry was 30 months (range: 5–178). Nineteen patients were in remission and eight had progressive disease. MM therapy at study entry and through the follow up period included: revlimid (n=12), carflizomib (n=2). Therapy of PN included narcotics (n=13), gabapentin (12), amitriptyline (n=3), pregabalin (n=2), and duloxetine (n=2). Two patients withdrew after first acupuncture treatment. Twenty-five patients were evaluable for response after completion of 3 weeks (n=2), 6 weeks (n=2), 9 weeks (n=1) and 10 weeks of acupuncture (n=20). There were no adverse events associated with the acupuncture treatment. All but five patients maintained the same dose of pain medications throughout the study. Fourteen patients (56%) reported improved daily functions (e.g. walking and coordination). Ten patients (40%) reported > 50% decrease in average NPS during treatment (week 1–10). Seven patients reported 50% reduction in FACT/GOG-Ntx total scores. The decrease in NPS and FACT/GOG-Ntx, was statistically significant between baseline assessments through week 14; p-values are 0.001 and <0.0001, respectively. On the other hand, TNSc scores, an objective clinical assessment, did not significantly change. Of fifteen patients who had nerve conduction studies, five showed >10% increase in motor nerve amplitude but there was no correlation observed between symptoms/function improvements and nerve conduction studies. At week 8, one of the measured cytokines: Brain-Derived Neurotrophic Factor (BDNF) significantly increased from baseline (p=0.043). This increase was no longer significant at week 14 follow up. Conclusions: Acupuncture is safe and effective in treating persistent moderate to severe BIPN, with improvements of patient reported outcomes, pain and function. The increased BDNF during acupuncture treatment suggests that acupuncture may work through promoting survival and growth of neurons, a lengthy process that may explain the lag of significant objective clinical improvement in the short follow up of our patients. The mechanism of acupuncture working through BDNF to treat PN warrants further evaluation. Disclosures: No relevant conflicts of interest to declare.

A quitosana, polissacarídeo linear obtido a partir do exoesqueleto de crustáceos e artrópodes, tem sido pesquisada em Odontologia por suas diversas propriedades terapêuticas. O objetivo do presente estudo foi realizar uma revisão da literatura sobre as aplicações atuais e as possibilidades terapêuticas da quitosana na odontologia. A busca foi realizada através do banco de dados eletrônico do Pubmed, utilizando os descritores Quitosana, Odontologia e Biomateriais. Foram incluídas pesquisas científicas utilizando quitosana em diversas áreas da odontologia e excluídas revisões de literatura e estudos não odontológicos, sendo selecionados 13 artigos. A quitosana induz resposta transcricional e anti-inflamatória em fibroblastos gengivais sobre citocinas inflamatórias, fatores transformadores do crescimento (TGF -β) e fatores de crescimento tumoral (TNF-α) que estão diretamente relacionados à atividade patológica periodontal. Nas infecções endodônticas persistentes, a substância atua criando ligações de hidrogênio e liberação de íons cálcio, o que potencializa a ação dos irrigadores intracanal, além de causar menos estresse oxidativo. Para a odontologia restauradora, a quitosana demonstrou eficácia como auxiliar no condicionamento da dentina e mostrou potencial para induzir a migração de odontoblastos na proteção do complexo dentino-pulpar. A substância atua como uma cura de feridas orais devido à sua capacidade de estimular a formação de fibroblastos e novos vasos sanguíneos, além de células anti-inflamatórias. Descritores: Biopolímeros; Biomateriais; Biotecnologia. Referências Zhao X, Li P, Guo B, Ma PX. Antibacterial and conductive injectable hydrogels based on quaternized chitosan-graft-polyaniline/oxidized dextran for tissue engineering. Acta Biomater. 2015;26:236-48. Tomihata K, Ikada Y. In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials. 1997;18(7):567-75 Citgez B, Cengiz AN, Akgun I, Uludag M, Yetkin G, Bahat N, Ozcan O, Polat N, Akcakaya A, Karatepe O. Effects of chitosan on healing and strength of colonic anastomosis in rats. Acta Cir Bras. 2012;27(10):707-12. Azevedo VVC, Chaves SA, Bezerra DC, Lia Fook MV, Costa ACFM. Quitina e Quitosana: aplicações como biomateriais. Rev Eletr Mater Proc. 2007;2(3):27-34. Tavaria FK, Costa EM, Pina-Vaz I, Carvalho MF, Pintado MM. A quitosana como biomaterial odontológico: estado da arte. Rev Bras Eng Bioméd. 2013;29(1):110-20. Ueno H, Nakamura F, Murakami M, Okumura M, Kadosawa T, Fujinag T. Evaluation effects of chitosan for the extracellular matrix production by fibroblasts and the growth factors production by macrophages. Biomaterials. 2001;22(15):2125-30. Shahid F, Abuzaytoun R. Chitin, chitosan, and co-products: chemistry, production, applications, and health effects. Adv Food Nutr Res. 2005;49(1):93-135. Croisier F, Jerome C. Chitosan-based biomaterials for tissue engineering. Eur Polym J. 2013;49(1):780-92. Giovino C, Ayensu I, Tetteh J, Boateng JS. An integrated buccal delivery system combining chitosan films impregnated with peptide loaded PEG-b-PLA nanoparticles. Colloids Surf B Biointerfaces. 2013;112(1):9-15. Wieckiewicz M, Boening KW, Grychowska N, Paradowska-Stolarz,U. Clinical Application of Chitosan in Dental Specialities. Mini Rev Med Chem. 2017;17(5):401-9. Ravi Kumar MNV. A análise dos pedidos de quitina e quitosana. R React Funct 2000;46(1):1-27. Chen CK, Chang NJ, Wu YT, Fu E, Shen EC, Feng CW, Wen ZH. Bone Formation Using Cross-Linked Chitosan Scaffolds in Rat Calvarial Defects. Implant Dent. 2018;27(1):15-21 Pavez L, Tobar N, Chacon C, Arancibia R, Martinez C, Tapia et al. Chitosan triclosan particles modulate inflammatory signaling in gingival fibroblasts. J Periodontal Res. 2017; 53(2):232-39. Moraes PC, Marques ICS, Basso FG, Rosseto HL, Pires de Sousa FCP, Costa CAS et al. Repair of Bone Defects with Chitosan- Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement. Braz Dent J. 2017;28(3):287-95. Aydin UZ, Akpinar KE, Hepokur C, Erdönmez D. Assessment of toxicity and oxidative DNA damage of sodium hypochlorite, chitosan and propolis on fibroblast cells. Braz Oral Res. 2018;32(1):1-8. Özdoğan AI, Ilarslan YD, Kösemehmetoğlu K, Acka G, Kutlu HB, Comerdov E et al. In Vivo Evaluation of Chitosan Based Local Delivery Systems for Atorvastatin in Treatment of Periodontitis. Int J Pharm. 2018;25(1):470-76. Paiola FG, Lopes FC, Mazzi-Chaves JF, Pereira RD, Oliveira HF, Queiroz AM et al. How to improve root canal filling in teeth subjected to radiation therapy for câncer. Braz Oral Res. 2018;32(1):1-9. Farhadian N, Godiny M, Moradi S, Hemati Azandaryani A, Shahlaei M. Chitosan/gelatin as a new nano-carrier system for calcium hydroxide delivery in endodontic applications: Development, characterization and process optimization. Mater Sci Eng C Mater Biol Appl. 2018;92:540-46. Subhi H, Reza F, Husein A, Al Shehadat SA, Nurul AA. Gypsum-Based Material for Dental Pulp Capping: Effect of Chitosan and BMP-2 on Physical, Mechanical, and Cellular Properties. Int J Biomater. 2018;2018:3804293. Soares DG, Anovazzi G, Bordini EAF, Zuta UO, Silva Leite MLA, Basso FG, Hebling J, de Souza Costa CA. Biological Analysis of Simvastatin-releasing Chitosan Scaffold as a Cell-free System for Pulp-dentin Regeneration. J Endod. 2018;44(6):971-76. Işılay Özdoğan A, Akca G, Şenel S. Development and in vitro evaluation of chitosan based system for local delivery of atorvastatin for treatment of periodontitis. Eur J Pharm Sci. 2018;124:208-16. Kesim B, Burak AK, Ustun Y, Delikan E, Gungor A. Effect of chitosan on sealer penetration into the dentinal tubules. Niger J Clin Pract. 2018;21(10):1284-90. Guo JM, Makvandi P, Wei CC, Chen JH, Xu HK, Breschi L, Pashley DH, Huang C, Niu LN, Tay FR. Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen. Acta Biomater. 2019;90:424-40. Susanto A, Susanah S, Priosoeryanto BP, Satari MH, Komara I. The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect. J Indian Soc Periodontol. 2019;23(2):113-18.

Summary Densely-fractured oil-wet carbonate fields pose a true challenge for oil recovery that traditional primary and secondary processes fail to meet. The difficulty arises from the combination of two unfavorable characteristics: First, the dense fracturing frustrates an efficient waterflood; second, because of the oil-wetness, the water pressure exceeds the oil pressure inside the matrix blocks, thus inhibiting spontaneous imbibition of water. In the past decade, using a new class of surfactants, enhanced oil recovery (EOR) researchers have studied the options to chemically revert the wettability of carbonate rock without drastically decreasing the oil-water interfacial tension. These chemicals, termed "wettability modifiers" (WMs), effectively reverse the sign of capillary pressure at the prevalent saturation. With the oil pressure exceeding the water pressure, the capillary pressure becomes the driving force for oil expulsion from the matrix and into the fracture system. Previous publications on chemical wettability modification focused on the performance of different chemical wettability modifiers for a chosen rock/oil/brine system. In some cases, they demonstrated an almost full oil recovery from core plugs. Little attention, however, has been given to the mechanism underlying the transport of the chemical into the matrix block and to the proper scaling of laboratory results to reservoir size. The present study aims to demonstrate that imbibition after wettability modification is diffusion-limited. To this end, the recovery profiles for spontaneous capillary imbibition, as well as for imbibition after wettability modification, are calculated. The results are then used to compare with the data of Amott cell imbibition experiments. It is confirmed that in both cases, the cumulative recovery is initially proportional to the square root of time. Imbibition after wettability modification, however, takes approximately 1,000 times longer than spontaneous capillary imbibition into a water-wet medium. The slow recovery observed in the case of imbibition after wettability modification is in excellent agreement with the assumption that, in the absence of significant spontaneous imbibition, the WM, to unfold its action, must first diffuse into the porous medium. In any diffusion process, the time scale is linked to the square of the length scale of the medium. Therefore, it would take up to 1,000 times longer (an equivalent of 200 years) before the same recovery is obtained from a meter-scale matrix block as is obtained from a centimeter-scale plug in a laboratory in 100 days. Consequently, unless a significantly faster transport mechanism for the wettability modifier is identified, or unless viscous forces or buoyancy enable forced imbibition, the chemical wettability modification of fractured oil-wet carbonate rock does not provide an economically interesting opportunity. Introduction Rock fractures provide comparatively highly permeable flow paths through oil reservoirs. In a densely fractured reservoir, the permeability contrast between the fracture network and the oil-bearing matrix can be significant. In that case, the viscous pressure differential across individual matrix blocks can be too small to release oil from the blocks under waterflood, thus leading to a poor recovery. Depending on the wetting state of the matrix and its initial water saturation, Swi, capillary action can cause imbibition of water up to a "spontaneous" equilibrium saturation, commonly denoted as Sspw. At this saturation, however, the capillary pressure inside the matrix block coincides with that in the fracture, and the recovery ceases. Experience has shown that carbonate fields often range from intermediate-wet to preferentially oil-wet (Treiber et al. 1972; Chilingar and Yen 1983), which is synonymous with Sspw being close or equal to Swi ; thus, they exhibit very limited recovery during primary and secondary production. Recently, a new EOR technique, designed specifically to tackle the challenges outlined previously, has been suggested by Austad and coworkers (Austad and Milter 1997; Standnes and Austad 2000a, b). In their pioneering work, these authors show that certain chemicals, when dissolved in the surrounding brine, can initiate water imbibition into oil-saturated core plugs and, hence, lead to the recovery of oil. One possible mechanism that explains these observations is the solubilization of adsorbed hydrocarbon components from the pore surface—as demonstrated by an atomic force microscopy study by Kumar et al. (2005), this exposes the intrinsically hydrophilic matrix. Another possibility is the formation of an additional chemical layer covering the adsorbed hydrophobic material. In either case, the pore surface becomes more hydrophilic, and the wettability of the matrix is thus modified. In a capillary rise experiment into parallel plates, Kumar et al. also observed different time scales for different types of wettability-modifying chemicals (2005). Using the cationic wettability modifier dodecyl trimethyl ammonium bromide (DTAB, also known as C12TAB), Standnes and Austad deduced that wettability modification was achieved through the comparatively slow process of partitioning the chemical into the oil phase, followed by desorption and solubilization of anionic hydrocarbon components (2000a, b). Shen et al. (2006) and Rao et al. (2006) measured the effect of surfactants on the relative water/oil permeabilities at different interfacial tensions. Wu et al. (2006) studied the properties and ranked the efficiency of chemical model compounds, based on their chemical structure, to modify the wettability and enhance recoveries. Several groups have taken initiative to model wettability modification in numerical simulators (Adibhatla et al. 2005; Delshad et al. 2006). So far, no significant attention has been given to time dependence and to the subsequent upscaling of the laboratory results to matrix block scale. This subject will be addressed in the present work. The structure of the article is as follows: In the Theory section, the basic results for countercurrent capillary imbibition will be briefly reviewed and compared to Fick's law of molecular diffusion. The oil recovery as a function of time for both capillary imbibition and imbibition after wettability modification will be predicted. The experimental approach to imbibition at different wetting situations will be described in the section Materials and Preparation. The recovery results will then be analyzed using the previously derived equations. Finally, tentative conclusions for the upscaling will be drawn.

Green gram (Vigna radiata) is considered the chief legume in Pakistan. Thus, current study was conducted to examine the ameliorating effect of phytohormones pre-treatments under salt stress on proteome profile of green gram by sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The soluble green gram seedlings proteins were resolved on 4% stacking and 12% resolving gels. The SDS-PAGE resolved 24 polypeptide bands ranging from 200 to 17kDa. Among these, 12 out of 24 bands of proteins were essentials house-keeping or growth proteins of green grams. While, 120, 114.6, 51.8, 29.1, and 22.8 kDa bands were over-expressed under 50 to 350mM salt with phytohormones treatments. The others 104.5 kDa, 99.8 kDa, 95.3 kDa, 91.0 kDa, 55 kDa, 46 kDa, and 17kDa bands were related to the GAᴣ, IAA, and SA induced tolerance. Overall 120 kDa, 114.6 kDa, 104.5 kDa, 99.8, 95.3 kDa, 51.8 kDa, 29.1 kDa and 22.8kDa bands were first time identified in the current study. The information retrieved from NCBI protein database, the resolved peptides were principally belonging to 7S and 8S vicilin, 2S, 8S, 11S, and 16.5S globulins. It is determined that seed priming with SA enhanced tolerance in green gram by rapidly synthesizing stress alleviating peptides.Key word: Cluster analysis, dendrogram, mungbean, salt stress, SDS-PAGEINTRODUCTIONVarious world-wide health concerning organization recommended the use of high graded plant protein such as legumes to prevent the risk of metabolic disorder (Hou et al., 2019). Legumes are most important protein crop on the earth. Among the legumes, the green gram is the major pulses. Its seeds are rich in superior quality storage protein, which account 85% of the total protein while, another 15% have not been broadly studied (Yi-Shen et al., 2018). The soluble storage protein comprises of 60% globulins, 25% albumin and 15% prolamins. Globulins are further divided into 3.4% basic-type (7S), 7.6% legumin-type (11S), and 89% vicilin-type (8S) (Mendoza et al., 2001; Itoh et al., 2006). Other than proteins, the green gram seeds also contain starch, fiber, phenolic compound, saponins, vitamins, calcium zinc, potassium, folate, magnesium, manganese and very low in fat that made it meager man’s meat (Hou et al., 2019). It is also a good source of green manure and fodder (El-Kafafi et al., 2015). Its root has ability to fix 30 to 50 Kg/ha atmospheric nitrogen in the soil which is essential for maintaining soil fertility (Chadha, 2010). The green gram is the valuable and the major Rabi pulse crop of Pakistan. Its cultivation area in 2016-2017 was about 179,000 hectares with seed yield of 130,000 tones. In comparison during 2017-2018, it was cultivated on 161,800 hectares land with 118,800 tones seed yield (GOP, 2018). One of the reasons of this 9% decrease in both land and productivity is the shortage of irrigated land due to soil salinity. The salinity induce oxidative bust in the mungbean cells, caused by responsive oxygen species (ROS) such as hydrogen peroxide, singlet oxygen, hydroxyl radical and superoxide radical. The ROS create hindrance in various metabolic processes of plant via interacting with macromolecules like proteins (Alharby et al., 2016). However, phytohormones like gibberellic acid (GAᴣ), indole acetic acid (IAA), and salicylic acid (SA) take part in the biosynthesis of salt tolerance proteins under salinity. These salt tolerance proteins acclimate plants under salinity stress. Application of biotechnology plays a significant role in agriculture (Khan et al., 2017). Therefore, production of particular proteins under salt stress is a specific response of cell which can be analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is the simple, valid, and cost-effective biochemical marker (Mushtaq et al., 2018). This marker has been widely used to determine the extent of evolutionary variations in crops (El-Kafafi et al., 2015).OBJECTIVES The present study was directed first time with the aim to investigate the toxic effect of sodium chloride (0-350 mM) and stress acclimation by pre-treatment of GAᴣ, IAA, and SA on the proteome profile of NM-92 cultivar of a Pakistani green gram.MATERIALS AND METHODSThe present study was replicated thrice in the plant laboratory of Department of Genetics, Faculty of Science, and University of Karachi. The seeds of mung bean cultivar NM-92 were acquired from National Agricultural Research Centre (NARC), Islamabad. These freshly collected 15 seedsˉ1 treatment / replication were divided into two sets. The first was named as sodium chloride (SC) stress treatments were imbibed in sterile distilled water (DW) whereas, second set soaked in gibberellic acid (GAᴣ) (BDH Chemicals, England), indole acetic acid (IAA) (Fluka, Switzerland), and salicylic acid (SA) (J.T. Baker, Holland) in the separate beaker for 24 hours under dark condition. After 24 hours, given ample time to both the sets at room temperature. After recovery, all 20 treatments were sown in the 150 X 30 mm sized petri-dishes containing 0, 50, 150, 250, and 350 millimolar (mM) sodium chloride solution (Fisher Scientific, UK) for 72 hours.Protein extraction: Protein extraction was done by taking 0.3g of seedlings in an ice chilled mortar and crushed by adding 600µL 0.2 M Tris-HCl buffer having pH 7.5 contained 5% SDS (w/v) and 5% 2-mercaptoethanol (v/v). The homogenate was incubated at 0oC for 30 min., boiled in the water bath for 3 min. at 100oC. Samples were centrifuged in Heraeus Biofuge D-37520, Germany for 30 min. at 8000 rpm. The protein supernatant was saved at below 0°C for quantitative and qualitative determination with minor modifications. The total soluble protein content of the samples was estimated via “Bovine Serum Albumin (BSA) standard curve” and explicit in µg protein milligramˉ1 fresh weight of mung seedlings.Bovine serum albumin standard curve (2000 μg/mL): Total protein standard curve was made by dissolving 0.05g of Bovine Serum Albumin (BSA) in 25mL of distilled water. Ten serial dilutions were made from 0.1 mL to 1mL by BSA solution then performed Lowry. A standard curve of total proteins was plotted by taking BSA absorbance at Y-axis and 2000 μg BSA / mL at X-axisSample preparation for SDS-PAGE: For qualitative assessment of total proteins; the 35μL of saved protein supernatant was combined with 15μL of sample diluting buffer (SDB). The SDB was made up of 0.0625 M Tris-HCl pH 6.8 with 2% of SDS, 10% of glycerol, 0.003% of bromophenol blue dye and 5% of 2-mercaptoethanol. Boil the 50μL protein SDB supernatant at 100oC in water bath for 3 min., centrifuged at 6000 rpm for 4 min. The supernatant was loaded on SDS-PAGE gel with the given formulae. The SDS- PAGE: Total proteins were fractionated via SDS-PAGE with 4% stacking and 12% resolving gel. The resolving gel of 12% was made by taking 6mL solution A, 1.8 mL 3 M Tris 1 M HCl buffer pH 8.8, 144μL 10% SDS, 5.74 mL sterile distilled water, 720μL 1.5% ammonium persulphate (APS) in deionized water and 10μL TEMED. While, stacking was composed of 1.25mL of solution A, 2.5mL of 0.5M Tris 1M HCl buffer pH 6.8, 100μL 10% SDS, 1.8 mL of distilled water, 500μL 1.5% APS and 12μL TEMED. Solution A was prepared by conjoining 30% acrylamide and 0.8% N, N’-methylene-bisacrylamide in deionized water. To avoid polymerization in the beaker; the prepared solution was quickly poured into the 3 mm thick gel plates after adding TEMED. The stacking was lined over resolving gel, then combs were inserted between the gel plates of SCIE-PLAS TV-100 separation system, UK, and allowed to polymerize for ½ an hour. After polymerization gel was placed in the tank which were filled with Tris-Glycine buffer (electrode buffer) pH 8.4 then combs were removed. The electrode buffer contained 0.3% Tris, 1.41% Glycine and 0.1% SDS in 2000mL d/w. The gel was pre-run for 15 min. at 60 volts and 120 mA currents. The prepared SDS-PAGE samples were loaded in wells with BlueStepTM Broad Range Protein Marker, AMRESCO, USA as standard and run at 60 volts & 120 mA for about 45 min. When samples entered in resolving gel, and then gave 100 volts and 200 mA currents for around 2.5 hours. Furthermore, electrophoresis was carried out at a constant watt.The Gel was washed with 30% ethanol on Uni Thermo Shaker NTS-1300 EYELA, Japan at the constant shaking for 30 min. Then gels were placed in 10% glacial acetic acid in 50% methanol solution (Fixative) for 24 hours. SDS Gel was stained until protein bands were visible thereat placed as 5% of Methanol in 7.5% acetic acid glacial solution to destain the bands background. SDS-PAGE stain composed of 0.125% coomassie brilliant blue R-250 dissolved in 40% of Methanol and 7% acetic acid glacial solution. The stain was stirred on Magnetic stirrer & hot plate M6/1, Germany for 6-10 hours before used. Photographs were taken by Sanyo digital camera VPC-T1284BL and bands were scored through numbering pattern. Gels preserved in 10% acetic acid solution at 4°C.Interpretation of bands and data analysis: The total soluble protein bands relative mobility calculated by below formulae and Dendrogram was constructed via SPSS v. 20Where,F=(Migrated distance of protein band)/(Migrated distance of dye front)Slop=(Log MW of protein marker lower limit band–log〖MW of protein marker upper limit band )/(RF protein marker lower limit band –RF of protein marker upper limit band)RESULTS:The total soluble proteins extracted from green gram were perceived by SDS-PAGE Blue StepTm broad range biochemical markers. The protein-based marker was used to evaluate the toxic effect of sodium chloride along with pre-treatments of GAᴣ, IAA, and SA on proteome assay. In the current work, seedlings total soluble proteome resolved 24 polypeptide bands ranging from 200 to 17.1 kDa were recognized by using SDS-PAGE. The figure 1 showed Dendrogram assay, which classified the 20 treatments of SC, GAᴣ, IAA and SA into two major clusters where, the cluster I was the largest one (figure 1). Cluster I consisted of 15 treatments that further divided into I-A, and I-B. The pre-treatments of SC50+SA, SC150+SA, SC250+SA, and SC350+IAA were grouped together into C-1 of sub-cluster I-A. The C-2 of sub-cluster I-A, pre-treatment SC350+SA was most diverse among 20 treatments. The C-1 treatments showed 99% homology when compared with each other while, it was 97% similar with C-2. The sub-cluster I-B comprised another 10 treatments, SC0+GAᴣ, SC50+GAᴣ, SC150+GAᴣ, SC250+GAᴣ, SC350+GAᴣ, SC0+IAA, SC50+IAA, SC150+IAA, SC250+IAA, and SC0+SA that were also 99% similar for total proteins. Sub-cluster I-B pre-treatments was exhibiting 94% homology with the sub-cluster I-A. The second cluster was the smallest one that was divided into two sub-clusters, II-A and II-B. The II-A was comprised of SC50, SC150, and SC250 while, sub-cluster II-B consisted of SC0 and SC350. Within each sub-cluster, pre-treatments expressed 99% homology whereas, II-A was 97 different from II-B. Furthermore, cluster I showed 75% similarities with cluster II (figure 1). The seedlings storage proteome profile of green gram was shown in table 1.The results showed that 120kDa, 114.6 kDa, 51.8 kDa, 29.1 kDa and 22.8 kDa proteins bands were not induced at 0 mM SC, GAᴣ, IAA, and SA. The table 1 depicted the presence of 120 kDa and 114.6 kDa bands only at 350 mM SC level with all phytohormones treatments. Similarly, 51.8 kDa protein bands were appearing at 150SC, 250SC and 350SC stress with phytohormones. Based on the information collected from the NCBI protein database, this peptide was related to the 8S globulin alpha subunits. The two other, 7S globulins sub-units having 29.1kDa and 22.8 kDa molecular weights bands were synthesized under 50mM, 150mM, 250mM, 350mM SC stress with phytohormones. Concerning protein polypeptide of molecular weight 104.5 kDa, 99.8 kDa, 91.0 kDa, 55.0 kDa, and 46.0 kDa, those were induced by GAᴣ, IAA and SA at 0 to 350 mM SC. While, 17kDa protein band was appearing in SA, and IAA treated samples and 95.3kDa band was only present in SA treatment. Other 12 protein bands were present in all treatments proved as house-keeping proteins of green gram (table 1).DISCUSSIONThe SDS-PAGE profiling for proteome is the reliable and applied biochemical approach that has been used as biochemical marker in various crop differentiation, and characterization. In the current study, first time SDS-PAGE was utilized to investigate the impact of GAᴣ, IAA, and SA pre-soaking on green gram under salt toxicity. The salt toxicity adversely affects all seed, seedling, and plant metabolic process (Parveen et al., 2016). At salt toxicity, the endogenous GAᴣ, IAA, and SA levels markedly decrease (El-Khallal et al., 2009). In such condition, exogenous application of GAᴣ, IAA, and SA enhance seedlings survival rate by increasing synthesis of seed storage proteins. Likewise, our Dendrogram characterization based on 20 treatments showed significant diversity under 0 to 350 mM SC stress. The salicylic acid treatments were grouped together except SC0+SA treatment, exhibiting a close relationship, which proved its acclimating role under salt stress. These findings will help plant breeder toward enhancing food quality and quantity of green gram in future breeding programme on saline sodic land.The SDS-PAGE assay revealed 200. kDa, 109.4 kDa, 77 kDa, 68 kDa, 49 kDa, 38 kDa, 33 kDa, 26 kDa, 24 kDa, 22 kDa, 21 kDa and 19 kDa fractions as essential green gram proteins. Among these, 68 kDa, 49 kDa, 33 kDa, 26 kDa, 24 kDa and 21 kDa peptides were seed biotinylated isoform protein (Riascos et al., 2009), putative NADH-ubiquinone oxidoreductase subunit H (Gostinčar et al., 2019), heat shock protein 33 (Hamidian et al., 2015), globulin protein, seed coat / maturation protein (Dhaubhadel et al., 2005), and protein for dimerization. While, 22 kDa proteins belonged to the class of prolamin alpha zein Z1C1_2, Z1C1_4, and Z1C1_8 precursors, and 19kDa peptide was related with Z1A1_2, Z1A2_2, and Z1B_6 precursors (Miclaus et al., 2011). Further, the 91 kDa peptide is sucrose synthase SS1 protein, and 77kDa protein is the NADPH-cytochrome P450 reductase (Wang et al., 2004). Also, the phosphatase-associated two other proteins having 46 and 55 kDa molecular weight were reported earlier in Mucuna pruriens. Hameed et al. (2012) and Malviya et al. (2008) found 55 and 46kDa peptides as 7S vicilin small sub-units and 17kDa as 11S globulins sub-unit in the studied Vigna radiata. Some other molecular weight proteome such as 68 kDa and 49kDa are 7S vicilin, 33kDa is 8S vicilin, 38 and 26kDa 8S globulins, 24kDa 11S globulins, and 22kDa 16.5S globulins. These proteins required for germination and seed establishment of green gram plant (Hameed et al., 2012).The vast accumulation of 23kDa and 22kDa peptides under salt stress by salicylic acid, were reported previously in the mangrove Bruguiera parviffora and Zea mays (El-Khallal et al., 2009). Correspondingly, El-Kafafi et al. (2015) reported the presence of 115kDa, 23kDa, and 22kDa bands in the salt tolerant lines of green gram. These proteomes induced under salt stress may play a pivotal part in the stress acclimation and osmotic adjustment. Similarly, the induction of 104 kDa and 100kDa MW polypeptide by SC stress in the salt tolerant genotypes of green gram indicated the functional role of phytohormones in various metabolic and defense response El-Kafafi et al. (2015); Alharby et al. (2016), El-Khallal et al. (2009), Qados (2010). Ali et al. (2007), Alharby et al. (2016), and El-Kafafi et al. (2015) observed 17kDa, 26kDa, 33kDa and 77kDa bands involving in salt tolerance and can be considered as a positive biochemical marker for salt stress. Further, 26 kDa MW peptide also functions as osmotin under the salt stress that involved in enhancing the accumulation of glycine betaine and proline in the cells. Hence, proteome assay of green gram showed that GAᴣ, IAA, and SA could regulate the expression of salt stress proteins that are anticipated to play a crucial part in the salt tolerance mechanism. Likewise, the involvement of phytohormones in the induction of changes in the proteome profile pattern was attributed to their part in managing cell division by regulating some genes of apical meristems.CONCLUSIONFinally, the results revealed the presence of the ten new bands with MW of 200kDa, 120 kDa, 114.6 kDa, 109.4kDa, 104.5kDa, 99.8kDa, 95.3kDa, 51.8kDa, 29.1kDa and 22.8kDa have not reported previously under salt stress with phytohormones treatments in green gram. Furthermore, it was observed that phytohormones alleviate the negative impact of salt stress on green gram by enhancing synthesis of salt defense polypeptides. Hence, higher accumulation of proteins was observed in salicylic acid treated seedlings. Thus, present work recommended the pre-soaking of phytohormones to overcome the toxic impact of sodium chloride on green gram. 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Rapid urbanization rates impact significantly on the nature of Land Cover patterns of the environment, which has been evident in the depletion of vegetal reserves and in general modifying the human climatic systems (Henderson, et al., 2017; Kumar, Masago, Mishra, & Fukushi, 2018; Luo and Lau, 2017). This study explores remote sensing classification technique and other auxiliary data to determine LULCC for a period of 50 years (1967-2016). The LULCC types identified were quantitatively evaluated using the change detection approach from results of maximum likelihood classification algorithm in GIS. Accuracy assessment results were evaluated and found to be between 56 to 98 percent of the LULC classification. The change detection analysis revealed change in the LULC types in Minna from 1976 to 2016. Built-up area increases from 74.82ha in 1976 to 116.58ha in 2016. Farmlands increased from 2.23 ha to 46.45ha and bared surface increases from 120.00ha to 161.31ha between 1976 to 2016 resulting to decline in vegetation, water body, and wetlands. The Decade of rapid urbanization was found to coincide with the period of increased Public Private Partnership Agreement (PPPA). Increase in farmlands was due to the adoption of urban agriculture which has influence on food security and the environmental sustainability. The observed increase in built up areas, farmlands and bare surfaces has substantially led to reduction in vegetation and water bodies. The oscillatory nature of water bodies LULCC which was not particularly consistent with the rates of urbanization also suggests that beyond the urbanization process, other factors may influence the LULCC of water bodies in urban settlements. Keywords: Minna, Niger State, Remote Sensing, Land Surface Characteristics References Akinrinmade, A., Ibrahim, K., & Abdurrahman, A. (2012). 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Graphene-based composites have received a great deal of attention in recent year because the presence of graphene can enhance the conductivity, strength of bulk materials and help create composites with superior qualities. Moreover, the incorporation of metal oxide nanoparticles such as Fe3O4 can improve the catalytic efficiency of composite material. In this work, we have synthesized a composite material with the combination of reduced graphene oxide (rGO), and Fe3O4 modified tissue-paper (mGO-PP) via a simple hydrothermal method, which improved the removal efficiency of the of methylene blue (MB) in water. MB blue is used as the model of contaminant to evaluate the catalytic efficiency of synthesized material by using a Fenton-like reaction. The obtained materials were characterized by SEM, XRD. The removal of materials with methylene blue is investigated by UV-VIS spectroscopy, and the result shows that mGO-PP composite is the potential composite for the color removed which has the removal efficiency reaching 65% in acetate buffer pH = 3 with the optimal time is 7 h. Keywords Graphene-based composite, methylene blue, Fenton-like reaction. References [1] Ma Joshi, Rue Bansal, Reng Purwar, Colour removal from textile effluents, Indian Journal of Fibre & Textile Research, 29 (2004) 239-259 http://nopr.niscair.res.in/handle/123456789/24631.[2] Kannan Nagar, Sundaram Mariappan, Kinetics and mechanism of removal of methylene blue by adsorption on various carbons-a comparative study, Dyes and pigments, 51 (2001) 25-40 https://doi.org/10.1016/S0143-7208(01)00056-0.[3] K Rastogi, J. N Sahu, B. C Meikap, M. N Biswas, Removal of methylene blue from wastewater using fly ash as an adsorbent by hydrocyclone, Journal of hazardous materials, 158 (2008) 531-540.https://doi.org/10.1016/j.jhazmat.2008.01. 105.[4] Qin Qingdong, Ma Jun, Liu Ke, Adsorption of anionic dyes on ammonium-functionalized MCM-41, Journal of Hazardous Materials, 162 (2009) 133-139 https://doi.org/10.1016/j.jhazmat. 2008.05.016.[5] Mui Muruganandham, Rps Suri, Sh Jafari, Mao Sillanpää, Lee Gang-Juan, Jaj Wu, Muo Swaminathan, Recent developments in homogeneous advanced oxidation processes for water and wastewater treatment, International Journal of Photoenergy, 2014 (2014). http://dx. doi.org/10.1155/2014/821674.[6] Herney Ramirez, Vicente Miguel , Madeira Luis Heterogeneous photo-Fenton oxidation with pillared clay-based catalysts for wastewater treatment: a review, Applied Catalysis B: Environmental, 98 (2010) 10-26 https://doi.org/ 10.1016/j.apcatb.2010.05.004.[7] Guo Rong, Jiao Tifeng, Li Ruifei, Chen Yan, Guo Wanchun, Zhang Lexin, Zhou Jingxin, Zhang Qingrui, Peng Qiuming, Sandwiched Fe3O4/carboxylate graphene oxide nanostructures constructed by layer-by-layer assembly for highly efficient and magnetically recyclable dye removal, ACS Sustainable Chemistry & Engineering, 6 (2017) 1279-1288 https://doi.org/10.1021/acssuschemeng.7b03635.[8] Sun Chao, Yang Sheng-Tao, Gao Zhenjie, Yang Shengnan, Yilihamu Ailimire, Ma Qiang, Zhao Ru-Song, Xue Fumin, Fe3O4/TiO2/reduced graphene oxide composites as highly efficient Fenton-like catalyst for the decoloration of methylene blue, Materials Chemistry and Physics, 223 (2019) 751-757 https://doi.org/ 10.1016/j.matchemphys.2018.11.056.[9] Guo Hui, Ma Xinfeng, Wang Chubei, Zhou Jianwei, Huang Jianxin, Wang Zijin, Sulfhydryl-Functionalized Reduced Graphene Oxide and Adsorption of Methylene Blue, Environmental Engineering Science, 36 (2019) 81-89 https://doi. org/10.1089/ees.2018.0157.[10] Zhao Lianqin, Yang Sheng-Tao, Feng Shicheng, Ma Qiang, Peng Xiaoling, Wu Deyi, Preparation and application of carboxylated graphene oxide sponge in dye removal, International journal of environmental research and public health, 14 (2017) 1301 https://doi.org/10.3390/ijerph14111301.[11] Yu Dandan, Wang Hua, Yang Jie, Niu Zhiqiang, Lu Huiting, Yang Yun, Cheng Liwei, Guo Lin, Dye wastewater cleanup by graphene composite paper for tailorable supercapacitors, ACS applied materials & interfaces, 9 (2017) 21298-21306 https://doi.org/10.1021/acsami.7b05318.[12] Wang Hou, Yuan Xingzhong, Wu Yan, Huang Huajun, Peng Xin, Zeng Guangming, Zhong Hua, Liang Jie, Ren MiaoMiao, Graphene-based materials: fabrication, characterization and application for the decontamination of wastewater and wastegas and hydrogen storage/generation, Advances in Colloid and Interface Science, 195 (2013) 19-40 https://doi. org/10.1016/j.cis.2013.03.009.[13] Marcano Daniela C, Kosynkin Dmitry V, Berlin Jacob M, Sinitskii Alexander, Sun Zhengzong, Slesarev Alexander, Alemany Lawrence B, Lu Wei, Tour James M, Improved synthesis of graphene oxide, ACS nano, 4 (2010) 4806-4814 https://doi.org/10.1021/nn1006368.[14] Zhang Jiali, Yang Haijun, Shen Guangxia, Cheng Ping, Zhang Jingyan, Guo Shouwu, Reduction of graphene oxide via L-ascorbic acid, Chemical Communications, 46 (2010) 1112-1114 http://doi. org/10.1039/B917705A [15] Gong Ming, Zhou Wu, Tsai Mon-Che, Zhou Jigang, Guan Mingyun, Lin Meng-Chang, Zhang Bo, Hu Yongfeng, Wang Di-Yan, Yang Jiang, Nanoscale nickel oxide/nickel heterostructures for active hydrogen evolution electrocatalysis, Nature communications, 5 (2014) 4695 https:// doi.org/10.1038/ncomms5695.[16] Wu Zhong-Shuai, Yang Shubin, Sun Yi, Parvez Khaled, Feng Xinliang, Müllen Klaus, 3D nitrogen-doped graphene aerogel-supported Fe3O4 nanoparticles as efficient electrocatalysts for the oxygen reduction reaction, Journal of the American Chemical Society, 134 (2012) 9082-9085 https://doi.org/10.1021/ja3030565.[17] Nguyen Son Truong, Nguyen Hoa Tien, Rinaldi Ali, Nguyen Nam Van, Fan Zeng, Duong Hai Minh, Morphology control and thermal stability of binderless-graphene aerogels from graphite for energy storage applications, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 414 (2012) 352-358 https://doi.org/ 10.1016/j.colsurfa.2012.08.048.[18] Deng Yang, Englehardt James D, Treatment of landfill leachate by the Fenton process, Water research, 40 (2006) 3683-3694 https://doi.org/ 10.1016/j.watres.2006.08.009.

The MT-ATP8 gene encodes for A6L protein subunit belonging to the proton channel of the ATP synthase. MT-ATP8 gene’s mutations can affect the structure and function of the ATP synthase, which may cause diseases. In this study, alterations of MT-ATP8 gene were investigated in tumor tissues of patients with breast cancer and control blood samples using PCR combined with direct sequencing and PCR-RFLP methods, data were analyzed using bioinformatics tools and statistical methods. Sequencing results revealed 5 variants of MT-ATP8 gene on 35 breast tumor tissues and 26 blood samples of controls, of which two mutations C8414T and C8417T altered the amino acid sequence of the resulting protein. The C8417T was further screened by PCR-RFLP and was found in 0,98% (1/102) of breast tumor samples. This change lead to substitution of lecine to phenylalanine (L18F) in a highly conserved position of A6L and was predicted as probably damaging to the structure and function of the protein. Additionally, a 9 bp deletion was also observed in a non-coding region of mtDNA in 26,5% (27/102) of breast cancer patients and 27% (7/26) of controls. Thus, these results showed that C8417T variant in the conserved position of MT-ATP8 gene was rare and first identified in a group of breast cancer patients in Vietnam. Keywords Breast cancer, mitochondrial DNA, MT-ATP8 References [1] Petros JA, Baumann AK, Ruiz-Pesini E, Amin MB, Sun CQ, Hall J, Lim S, Issa MM, Flanders WD, Hosseini SH, Marshall FF, Wallace DC, mtDNA mutations increase tumorigenicity in prostate cancer, Proc Natl Acad Sci U S A (2005), 102(3):719-24.[2] Wang X, The expanding role of mitochondria in apoptosis, Genes Dev (2001), 15(22):2922-33.[3] Jonckheere AI, Smeitink JA, Rodenburg RJ, Mitochondrial ATP synthase: architecture, function and pathology, J Inherit Metab Dis (2012), 35(2):211-25.[4] Grzybowska-Szatkowska L, Slaska B, Rzymowska J, Brzozowska A, Florianczyk B, Novel mitochondrial mutations in the ATP6 and ATP8 genes in patients with breast cancer, Mol Med Rep (2014), 10(4):1772-8.[5] Adzhubei IA, Schmidt S, Peshkin L, Ramensky VE, Gerasimova A, Bork P, Kondrashov AS, Sunyaev SR, A method and server for predicting damaging missense mutations, Nat Methods (2010), 7(4):248-9.[6] Thapa S, Lalrohlui F, Ghatak S, Zohmingthanga J, Lallawmzuali D, Pautu JL, Senthil Kumar N, Mitochondrial complex I and V gene polymorphisms associated with breast cancer in mizo-mongloid population, Breast Cancer (2016), 23(4):607-16.[7] Warburg O, On the origin of cancer cells, Science (1956), 123:309-14.[8] Dumas JF, Rousse D, Servais S, Mitochondria and cancer, Cellular Bioenergetics in Health and Diseases: New Perspectives in Mitochondrial Biology (2012), 115-47.[9] Mkaouar-Rebai E, Kammoun F, Chamkha I, Kammoun N, Hsairi I, Triki C, Fakhfakh F, A de novo mutation in the adenosine triphosphatase (ATPase) 8 gene in a patient with mitochondrial disorder, J Child Neurol (2010), 25(6):770-5.[10] Jonckheere AI, Hogeveen M, Nijtmans LG et al., A novel mitochondrial ATP8 gene mutation in a patient with apical hypertrophic cardiomyopathy and neuropathy, J Med Genet (2008), 45:129-33.[11] Ware SM, El-Hassan N, Kahler SG et al., Infantile cardiomyopathy caused by a mutation in the overlapping region of mitochondrial ATPase 6 and 8 genes, J Med Genet (2009), 46:308-14.[12] Liu VW, Shi HH, Cheung AN, Chiu PM, Leung TW, Nagley P, Wong LC, Ngan HY, High incidence of somatic mitochondrial DNA mutations in human ovarian carcinomas, Cancer Res (2001), 61(16):5998-6001.[13] Zhuo G, Feng G, Leng J, et al., A 9-bp deletion homoplasmy in women with polycystic ovary syndrome revealed by mitochondrial genome-mutation screen, Biochem Genet (2010), 48:157-163.[14] Abu-Amero KK, Alzahrani AS, Zou M, Shi Y, Association of mitochondrial DNA transversion mutations with familial medullary thyroid carcinoma/multiple endocrine neoplasia type 2 syndrome, Oncogene (2006), 25:677-84.[15] Bonora E, Porcelli AM, Gasparre G, et al., Defective oxidative phosphorylation in thyroid oncocytic carcinoma is associated with pathogenic mitochondrial DNA mutations affecting complexes I and III, Cancer Res (2006), 66:6087-96.[16] Costa-Guda J, Tokura T, Roth SI, Arnold A, Mitochondrial DNA mutations in oxyphilic and chief cell parathyroid adenomas, BMC Endocr Disord (2007); 7:8.[17] Chintha R, Kaipa PR, Sekhar N, Hasan Q, Mitochondria and tumors: A new perspective, Indian J Cancer (2013), 50(3).[18] Tan DJ, Bai RK, Wong LJ, Comprehensive scanning of somatic mitochondrial DNA mutations in breast cancer, Cancer Res (2002), 62(4):972-6.[19] Tipirisetti NR, Lakshmi RK, Govatati S, Govatati S, Vuree S, Singh L, Raghunadha Rao D, Bhanoori M, Vishnupriya S, Mitochondrial genome variations in advanced stage breast cancer: a case-control study, Mitochondrion (2013), 13(4):372-8. [20] Ghaffarpour M, Mahdian R, Fereidooni F, Kamalidehghan B, Moazami N, Houshmand M, The mitochondrial ATPase6 gene is more susceptible to mutation than the ATPase8 gene in breast cancer patients, Cancer Cell Int (2014), 14(1):21.[21] Perucca-Lostanlen D, Narbonne H, Hernandez JB, et al., Mitochondrial DNA variations in patients with maternally inherited diabetes and deafness syndrome, Biochem Biophys Res Commun (2000), 277(3):771-5.[22] Bai Y, Guo Z, Xu J, Zhang J, Cui L, Zhang H, Zhang S, The 9-bp deletion at position 8272 in region V of mitochondrial DNA is associated with renal cell carcinoma outcome, Mitochondrial DNA A DNA Mapp Seq Anal (2014), 27(3):1973-5.[23] Jin Y, Yu Q, Zhou D, Chen L, Huang X, Xu G, Huang J, Gao X, Gao Y, Shen L, The mitochondrial DNA 9-bp deletion polymorphism is a risk factor for hepatocellular carcinoma in the Chinese population, Genet Test Mol Biomarkers (2012), 16(5):330-4.[24] Ren W, Li Y, Li R, Feng H, Wu S, Mao Y, Huang L, Mitochondrial intergenic COII/tRNA(Lys) 9-bp deletion, a biomarker for hepatocellular carcinoma? 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We review our recent work on optical biosensors based on microring resonators (MRR) integrated with 4x4 multimode interference (MMI) couplers for multichannel and highly sensitive chemical and biological sensors. The proposed sensor structure has advantages of compactness, high sensitivity compared with the reported sensing structures. By using the transfer matrix method (TMM) and numerical simulations, the designs of the sensor based on silicon waveguides are optimized and demonstrated in detail. We applied our structure to detect glucose and ethanol concentrations simultaneously. A high sensitivity of 9000 nm/RIU, detection limit of 2x10-4 for glucose sensing and sensitivity of 6000nm/RIU, detection limit of 1.3x10-5 for ethanol sensing are achieved. Keywords Biological sensors, chemical sensors, optical microring resonators, high sensitivity, multimode interference, transfer matrix method, beam propagation method (BPM), multichannel sensor References [1] Vittorio M.N. 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Leonardis, "Ammonia Optical Sensing by Microring Resonators," Sensors, vol. 7, pp. 2741-2749, 2007.[8] C. Lerma Arce, K. De Vos, T. Claes et al., "Silicon-on-insulator microring resonator sensor integrated on an optical fiber facet," IEEE Photonics Technology Letters, vol. 23, pp. 890 - 892, 2011.[9] Trung-Thanh Le, "Realization of a Multichannel Chemical and Biological Sensor Using 6x6 Multimode Interference Structures," International Journal of Information and Electronics Engineering, Singapore, vol. 2, pp. 240-244, 2011.[10] Trung-Thanh Le, "Microring resonator Based on 3x3 General Multimode Interference Structures Using Silicon Waveguides for Highly Sensitive Sensing and Optical Communication Applications," International Journal of Applied Science and Engineering, vol. 11, pp. 31-39, 2013.[11] K. De Vos, J. Girones, T. Claes et al., "Multiplexed Antibody Detection With an Array of Silicon-on-Insulator Microring Resonators," IEEE Photonics Journal, vol. 1, pp. 225 - 235, 2009.[12] Daoxin Dai, "Highly sensitive digital optical sensor based on cascaded high-Q ring-resonators," Optics Express, vol. 17, pp. 23817-23822, 2009.[13] Adrián Fernández Gavela, Daniel Grajales García, C. Jhonattan Ramirez et al., "Last Advances in Silicon-Based Optical Biosensors," Sensors, vol. 16, 2016.[14] Xiuyou Han, Yuchen Shao, Xiaonan Han et al., "Athermal optical waveguide microring biosensor with intensity interrogation," Optics Communications, vol. 356, pp. 41-48, 2015.[15] Yao Chen, Zhengyu Li, Huaxiang Yi et al., "Microring resonator for glucose sensing applications," Frontiers of Optoelectronics in China, vol. 2, pp. 304-307, 2009/09/01 2009.[16] Gun-Duk Kim, Geun-Sik Son, Hak-Soon Lee et al., "Integrated photonic glucose biosensor using a vertically coupled microring resonator in polymers," Optics Communications, vol. 281, pp. 4644-4647, 2008.[17] Carlos Errando-Herranz, Farizah Saharil, Albert Mola Romero et al., "Integration of microfluidics with grating coupled silicon photonic sensors by one-step combined photopatterning and molding of OSTE," Optics Express, vol. 21, pp. 21293-21298, 2013.[18] Trung-Thanh Le, "Two-channel highly sensitive sensors based on 4 × 4 multimode interference couplers," Photonic Sensors, vol. 7, pp. 357-364, 2017/12/01 2017.[19] Duy-Tien Le and Trung-Thanh Le, "Coupled Resonator Induced Transparency (CRIT) Based on Interference Effect in 4x4 MMI Coupler," International Journal of Computer Systems (IJCS), vol. 4, pp. 95-98, May 2017.[20] Trung-Thanh Le, "All-optical Karhunen–Loeve Transform Using Multimode Interference Structures on Silicon Nanowires," Journal of Optical Communications, vol. 32, pp. 217-220, 2011.[21] L.B. Soldano and E.C.M. Pennings, "Optical multi-mode interference devices based on self-imaging :principles and applications," IEEE Journal of Lightwave Technology, vol. 13, pp. 615-627, Apr 1995.[22] Trung-Thanh Le, Multimode Interference Structures for Photonic Signal Processing: LAP Lambert Academic Publishing, 2010.[23] J.M. Heaton and R.M. Jenkins, " General matrix theory of self-imaging in multimode interference(MMI) couplers," IEEE Photonics Technology Letters, vol. 11, pp. 212-214, Feb 1999 1999.[24] Trung-Thanh Le and Laurence Cahill, "Generation of two Fano resonances using 4x4 multimode interference structures on silicon waveguides," Optics Communications, vol. 301-302, pp. 100-105, 2013.[25] W. Green, R. Lee, and G. DeRose et al., "Hybrid InGaAsP-InP Mach-Zehnder Racetrack Resonator for Thermooptic Switching and Coupling Control," Optics Express, vol. 13, pp. 1651-1659, 2005.[26] Trung-Thanh Le and Laurence Cahill, "The Design of 4×4 Multimode Interference Coupler Based Microring Resonators on an SOI Platform," Journal of Telecommunications and Information Technology, Poland, pp. 98-102, 2009.[27] Duy-Tien Le, Manh-Cuong Nguyen, and Trung-Thanh Le, "Fast and slow light enhancement using cascaded microring resonators with the Sagnac reflector," Optik - International Journal for Light and Electron Optics, vol. 131, pp. 292–301, Feb. 2017.[28] Xiaoping Liang, Qizhi Zhang, and Huabei Jiang, "Quantitative reconstruction of refractive index distribution and imaging of glucose concentration by using diffusing light," Applied Optics, vol. 45, pp. 8360-8365, 2006/11/10 2006.[29] C. Ciminelli, F. Dell’Olio, D. Conteduca et al., "High performance SOI microring resonator for biochemical sensing," Optics & Laser Technology, vol. 59, pp. 60-67, 2014.[30] Trung-Thanh Le, "Two-channel highly sensitive sensors based on 4 × 4 multimode interference couplers," Photonic Sensors, pp. 1-8, DOI: 10.1007/s13320-017-0441-1, 2017.[31] O. A. Marsh, Y. Xiong, and W. N. Ye, "Slot Waveguide Ring-Assisted Mach–Zehnder Interferometer for Sensing Applications," IEEE Journal of Selected Topics in Quantum Electronics, vol. 23, pp. 440-443, 2017.[32] Juejun Hu, Xiaochen Sun, Anu Agarwal et al., "Design guidelines for optical resonator biochemical sensors," Journal of the Optical Society of America B, vol. 26, pp. 1032-1041, 2009/05/01 2009.[33] Y. Chen, Y. L. Ding, and Z. Y. Li, "Ethanol Sensor Based on Microring Resonator," Advanced Materials Research, vol. 655-657, pp. 669-672, 2013.[34] Sasikanth Manipatruni, Rajeev K. 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