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Dissertations / Theses on the topic 'Feruloil esterases'

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1

Lopes, Vânia Alexandra da Silva Cardoso. "High-Throughput production and characterization of Carbohydrate-Active enZYmes for animal nutrition." Doctoral thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, 2020. http://hdl.handle.net/10400.5/19760.

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Tese de Doutoramento em Ciências Veterinárias na especialidade Produção Animal<br>The biodegradation of plant cell wall (PCW) carbohydrates is performed by microbial enzymes that are generally referred to as CAZymes. In animal nutrition, it is now well established that the monogastric animals produce a limited repertoire of CAZymes and as such cannot use efficiently some dietary ingredients that sometimes display antinutritional properties. The dietary supplementation with exogenous CAZymes improves the nutritive value of diets and increases animal’s performance. In particular, this study demo
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2

Santos, Ana Paula Almeida dos. "Desenvolvimento de um método para a determinação da atividade de esterases usando material lignocelulósico como substrato." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/97/97132/tde-07112014-154425/.

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As feruloil esterases são enzimas relevantes para o processo de hidrólise de materiais lignocelulósicos oriundos de gramíneas. Estas enzimas são tradicionalmente determinadas com o emprego de substratos sintéticos de baixa massa molar. Entretanto, as atividades determinadas a partir desses ensaios não se correlacionam adequadamente com a ação das enzimas em substratos complexos e insolúveis como os materiais lignocelulósicos. Neste sentido, o presente trabalho visou desenvolver um método apropriado para medir a atividade de feruloil esterases utilizando um material lignocelulósico como substra
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Braga, Cleiton Márcio Pinto. "Produção das enzimas acessórias feruloil esterase e xilanase por fungos filamentosos isolados da região amazônica e sua aplicação na hidrólise do bagaço de canade-açúcar." Universidade Federal de São Carlos, 2013. https://repositorio.ufscar.br/handle/ufscar/7038.

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Made available in DSpace on 2016-08-17T18:39:51Z (GMT). No. of bitstreams: 1 6317.pdf: 1890056 bytes, checksum: dc49c9c95213e912864488dff97a859c (MD5) Previous issue date: 2013-12-13<br>Financiadora de Estudos e Projetos<br>Xylanase and feruloyl esterase (FAE) are two enzymes of great importance for plant biomass decomposition. The hemicellulose, one of the biomass constituents, has a great structural variety, so that for its complete deconstruction, several groups of enzymes are required, including mainly xylanases. These enzymes act on the &#946;-1,4 glycosidic bonds between the xylose res
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4

Alves, Luana de Fátima. "Criação de uma enzima multifuncional feruloil esterase/acetil-xilano esterase por desenho racional." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17131/tde-21072016-153003/.

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A parede celular das plantas inclui componentes polissacarídeos complexos, e a sacarificação destes polímeros necessita da ação de conjuntos de enzimas que atuem em sinergia. Enzimas podem formar complexos multi-enzimáticos que possuem mais de uma atividade catalítica derivada de domínios distintos de uma mesma cadeia polipeptídica. O objetivo deste trabalho foi construir uma enzima bifuncional com os domínios catalíticos: acetilxilano esterase (Axe) e feruloil esterase (Fae) para desconstrução de material lignocelulósico de cana-de-açúcar. Para isso, dois diferentes domínios catalíticos: acet
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5

Antonopoulou, Io. "Use of feruloyl esterases for chemoenzymatic synthesis of bioactive compounds." Licentiate thesis, Luleå tekniska universitet, Kemiteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-62836.

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Feruloyl esterases (FAEs, EC 3.1.1.73) represent a subclass of carboxylic acid esterases that under normal conditions catalyze the hydrolysis of the ester bond between hydroxycinnamic acids (ferulic acid, sinapic acid, caffeic acid, p-coumaric acid) and arabinose residues in plant cell walls. Based on their specificity towards monoferulates and diferulates, substitutions on the phenolic ring and on their amino acid sequence identity, they have been classified into four types (A-D). The use of FAEs as accessory enzymes for the degradation of lignocellulosic biomass and their synergism with othe
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6

Crepin, Valérie. "Ferulic acid esterases for effective processing of plant carbohydrates." Thesis, University of Nottingham, 2003. http://eprints.nottingham.ac.uk/28964/.

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Feruloyl esterases (E.C. 3.1.1.73), a subclass of the carboxylic acid esterases (E.C. 3.1.1.1), are able to hydrolyse the ester bond between the hydroxycinnamic acids and sugars present in the plant cell walls, and have been classified as Types A or B based on their substrate specificity for aromatic moieties. They constitute an interesting group of enzymes that have the potential for use over a broad range of applications in the agri-food industries. In order to expand the range of available enzymes, we have examined the production of feruloyl esterases by the filamentous fungi Talaromyces sl
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7

Rumbold, Karl 1973. "Isolation and properties of a feruloyl esterase from Aureobasidium pullulans and its mechanism in lignocellulose degradation." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53480.

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Dissertation (PhD)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: The production, purification and functional characterisation of feruloyl esterase from Aureobasidium pullulans were set as the primary objectives of this study. A further objective was to investigate a possible co-operative effect with other selected lignocellulolytic enzymes on substrates relevant to industry. In a comprehensive review, feruloyl esterases from various micro-organisms were compared both functionally and with regard to their primary structure, where applicable. Feruloyl esterases show intriguing
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8

Aliwan, Fraj O. "Mechanism, structure and specificity of a feruloyl esterase from Aspergillus niger." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267727.

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9

Castanares, Arantza. "Xylan-degrading enzymes from Penicillium pinophilum : purification and characterization of a feruloyl/ρ-coumaroylester esterase". Thesis, University of Aberdeen, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314624.

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Growth of <i>Penicillium pinophilum</i> in solid state culture on oat straw and wheat bran mixtures was found suitable for the production of xylan-degrading enzymes. An extracellular phenolic acid esterase and a xylanase were purified to homogeneity by a combination of anion-exchange and hydrophobic interaction chromatography. The physicochemical properties and the mode of action of the esterase on various xylan polysaccharides were investigated. The esterase had an apparent molecular mass of 57 kDa by SDS-PAGE and an isoelectric pH of 4.6. The enzyme released ferulic and ρ-coumaric acid from
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10

Faulds, C. B. "Ferulic acid esterases from Aspergillus niger : their application in the biotransformation of agricultural residues." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338044.

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11

Couto, Jessica. "Development of a novel biocatalytic approach for the synthesis of feruloylated glycosides by feruloyl esterase in selected multi-enzymatic extracts." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97035.

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Methyl ferulate and isolated feruloylated non-digestible oligosaccharides (NDOs) from sugar-beet pulp and wheat bran were used as substrates to evaluate the levels of feruloyl esterase (FAE) activity and substrate specificity of twenty multi-enzymatic preparations. The synthesis of feruloylated monosaccharides by FAEs expressed in the six best multi-enzymatic preparations from Bacillus spp. (Ceremix), Humicola spp. (Depol 740L), Aspergillus oryzae (Flavourzyme), Bacillus amyloliquefaciens (Multifect P 3000), Bacillus subtilis (RP-1), and Trichoderma reesei (Depol 670L) was investigated using a
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12

Tomaro-Duchesneau, Catherine. "Microencapsulated ferulic acid esterase active «Lactobacillus fermentum» for the reduction of inflammation and cholesterol in metabolic syndrome." Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=121276.

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Metabolic syndrome (MetS) is an important public health concern of industrialized countries. MetS is a cluster of metabolic disturbances, including abdominal obesity, atherogenic dyslipidemia, elevated blood pressure, insulin resistance and the presence of a proinflammatory and prothrombotic state. Currently, there is no successful therapeutic intervention for the prevention and treatment of this disorder. Chronic systemic inflammation and hypercholesterolemia are two important therapeutic targets of MetS. The goal of this work is to develop a novel microencapsulated ferulic acid-producing pro
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13

Hassan, Sozan. "Caractérisation de nouvelles enzymes impliquées dans la dégradation de polysaccharides végétaux à partir de la bactérie Dickeya dadantii 3937." Thesis, Lyon, INSA, 2011. http://www.theses.fr/2011ISAL0115.

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La bactérie phytopathogène Dickeya dadantii est responsable de la pourriture molle de nombreux végétaux. Elle sécrète dans le milieu extérieur toute une batterie d’enzymes capables de dégrader les constituants des parois végétales. La première partie de mon travail concerne les féruloyl estérases FaeD et FaeT. Les féruloyl estérases sont responsables de l’hydrolyse de liaisons ester entre l’acide férulique et les chaînes de xylane ou de pectine. En clivant ces liaisons, elles favorisent une dégradation complète de la paroi végétale. L'importance de ces enzymes nous a conduit à rechercher si D.
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14

McClendon, Shara Demetria. "Molecular design, construction, and characterization of a xylanosome: a protein nanostructure for biomass utilization." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/43610.

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Lignocellulosic biomass is an abundant renewable resource targeted for biofuel production. Cellulose and hemicellulose from biomass both contain fermentable sugars and other moieties that can be converted to biofuels or other commodity chemicals. Enzymatic hydrolysis of these biopolymers is a critical step in the liberation of sugars for fermentation into desired products. In nature, anaerobic microbes produce protein nanostructures called cellulosomes that efficiently degrade cellulose substrates by combining multiple enzyme activities onto a scaffolding protein. However, current enzyme c
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15

Kheder, Fadi. "Production et purification d'acide férulique estérases. Application à la synthèse d'esters phénoliques." Thesis, Vandoeuvre-les-Nancy, INPL, 2007. http://www.theses.fr/2007INPL075N/document.

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L’induction de la synthèse d’une acide férulique estérase (AFE) a été étudiée chez Streptomyces ambofaciens ATCC 23877. L’activité la plus élevée a été détectée en présence de son de blé désamidonné ou de xylane d’avoine (0,22, 0,21 mU/mg protéine, respectivement). Des productions d’AFE en bioréacteur ont également été réalisées en utilisant 1% (p/v) de son de blé comme inducteur. Le niveau de production de l’AFE a été trois fois plus important en bioréacteur qu’en fiole d’Erlenmeyer. L’AFE de Streptomyces ambofaciens ATCC 23877 et celle de Humicola sp., présente dans un mélange enzymatique co
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16

Ngobeni, Rhulani. "Functional characterisation of a novel ferulic acid esterase from Malawian hot spring metagenome." Thesis, 2011. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_6762_1338892842.

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17

Ciou, Yung-Ping, and 邱詠評. "Production of Thermostable Ferulic Acid Esterase from Thermobifida fusca and Its Application in Lignocellulose Degradation." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/33036370519882893535.

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