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Academic literature on the topic 'Feulgen'

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

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Journal articles on the topic "Feulgen"

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Ebnet, Klaus, and D. Vestweber. "Robert Feulgen Lecture 1998." Histochemistry and Cell Biology 112, no. 1 (July 20, 1999): 1–23. http://dx.doi.org/10.1007/s004180050387.

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Rasp, E., and J. E. Dumont. "1 Robert Feulgen Lecture 1991." Progress in Histochemistry and Cytochemistry 26, no. 1-4 (January 1992): 1–29. http://dx.doi.org/10.1016/s0079-6336(11)80074-4.

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Patil, Shankargouda, and Anveeta Agarwal. "Comparison and Evaluation of Mitotic Figures in Oral Epithelial Dysplasia using Crystal Violet and Feulgen Stain." Journal of Contemporary Dental Practice 15, no. 3 (2014): 273–77. http://dx.doi.org/10.5005/jp-journals-10024-1527.

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ABSTRACT Background Routine staining procedures often pose a problem in differentiating a mitotic cell from an apoptotic cell, deteriorating the reliability of histology grading. Although various new methods have been recommended for identifying mitotic figures (MFs) in tissues, the time factor and cost makes them less feasible. Thus, an attempt was made to evaluate the efficacy of crystal violet and Feulgen reaction in identifying MFs and also to see for any variation in the number of MFs in various grades of Epithelial dysplasia. Objectives: 1. Using crystal violet and Feulgen stain in the identification and counting of MFs on diagnosed cases of epithelial dysplasia and thereby to evaluate their efficacy. 2. To evaluate the variation in the number of MFs in various grades of epithelial dysplasia. Materials and methods The study sample includes retrieval of 30 formalin fixed paraffin embedded tissue sections diagnosed for different grades of epithelial dysplasia (WHO grading system, 2005) from the archives, Department of Oral Pathology, MSRDC, Bengaluru. Ten tissue sections each of mild, moderate and severe epithelial dysplasia were stained with H&E, Feulgen and 1% crystal violet stains and the number of MFs were counted. Five cases of cervical carcinoma were taken as control. Stained sections were compared, and data obtained was statistically analyzed using the Kruskal-Wallis test. Results A significant increase in the number of MFs (p = 0.02) was observed in Feulgen stained sections as compared to H&E stain. Conclusion Feulgen stain can be considered as a simple, reliable, cost-effective and reproducible method of staining MFs. How to cite this article Rao RS, Patil S, Agarwal A. Comparison and Evaluation of Mitotic Figures in Oral Epithelial Dysplasia using Crystal Violet and Feulgen Stain. J Contemp Dent Pract 2014;15(3):273-277.

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Lin, P., and D. C. Allison. "Measurement of DNA content and of tritiated thymidine and bromodeoxyuridine incorporation by the same cells." Journal of Histochemistry & Cytochemistry 41, no. 9 (September 1993): 1435–39. http://dx.doi.org/10.1177/41.9.8354883.

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We tested a method of measuring DNA content (Feulgen) and tritiated thymidine ([3H]-T) and bromodeoxyuridine (BrdU) incorporation by the same cell. Initial experiments showed that Feulgen hydrolysis denatured the DNA of fixed cells sufficiently to allow detection of incorporated BrdU with monoclonal antibodies. MCa-11 cells were then double-labeled with [3H]-T and BrdU, placed on slides, and Feulgen stained. Next, absorption cytometry was performed to measure the DNA content of randomly selected cells. Feulgen staining and the development and removal of either the [3H]-T or the BrdU grains after DNA measurements did not interfere with subsequent detection of the grains from the other label, and BrdU and [3H]-T can be used reliably in combination for identification of S-phase cells. This method may eventually allow the use of microscope-based image analysis to selectively measure the DNA contents and the BrdU/[3H]-T labeling of non-transformed stromal and cancer cells in solid tumors, thereby providing new insights into the growth kinetics of these heterogeneous cell populations.

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Foucrier, J., J. P. Rigaut, and D. Pechinot. "A combined AgNOR-Feulgen staining technique." Journal of Histochemistry & Cytochemistry 38, no. 11 (November 1990): 1591–97. http://dx.doi.org/10.1177/38.11.1698851.

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We describe a new staining technique (H-Ag-S) which allows observation and counting of active nucleolus organizer regions (NORs) and evaluation of the amount of DNA in the same cell nucleus. The procedure consists of combining a modified AgNOR staining method with the Feulgen reaction. A sequential procedure is proposed, based on the determination of optimal staining conditions. The technique, which was designed to allow studies of correlations between the transcriptional activity of rDNA genes and the cell ploidy, was primarily developed for rat liver smears. It should be applicable to most biological preparations, but the optimal conditions might be variable.

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Chieco, Pasquale, and M. Derenzini. "The Feulgen reaction 75 years on." Histochemistry and Cell Biology 111, no. 5 (May 20, 1999): 345–58. http://dx.doi.org/10.1007/s004180050367.

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Schulte, E., and D. Wittekind. "Standardization of the Feulgen-Schiff technique." Histochemistry 91, no. 4 (1989): 321–31. http://dx.doi.org/10.1007/bf00493008.

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Kesarkar, Kashmira, Avinash Tamgadge, Treville Peirera, Sandhya Tamgadge, Swati Gotmare, and Pooja Kamat. "Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains." Sultan Qaboos University Medical Journal [SQUMJ] 18, no. 2 (September 9, 2018): 149. http://dx.doi.org/10.18295/squmj.2018.18.02.005.

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Objectives: The objectives of this study were to quantitatively estimate the number of mitotic figures (MFs) and evaluate the cellular and nuclear features of various histological grades of oral squamous cell carcinoma (OSCC) using Feulgen and 1% crystal violet stains. Methods: This case-control study took place at the Dr D. Y. Patil Dental College & Hospital in Mumbai, Maharashtra, India, between June and December 2016. A total of 51 samples were retrieved from the hospital archives. Of these, 15 well-differentiated, 15 moderately-differentiated and six poorly-differentiated OSCC samples formed the case group while 15 samples of normal gingival mucosa constituted the control group. Each sample was dyed using Feulgen and 1% crystal violet stains and the mitotic count, nuclear area (NA), cellular area (CA), nuclear perimeter (NP), cellular perimeter (CP) and nuclear-to-cytoplasmic (N/C) ratio was calculated using computeraided morphometry techniques. Results: The number of MFs visible per field was significantly higher in Feulgen-stained sections as compared to those stained with crystal violet (P = 0.050). In addition, the NA, NP, CA and CP values and N/C ratios of samples in the experimental group increased significantly in accordance with an increase in OSCC grade (P <0.001). Conclusion: The Feulgen stain is more reliable than 1% crystal violet in terms of the selective staining of MFs. Moreover, the findings of this study indicate that computer-based morphometric analysis is an effective tool for differentiating between various grades of OSCC.Keywords: Crystal Violet; Feulgen Stain; Mitotic Index; Image Cytometry; Squamous Cell Carcinoma; Oral Cancers.

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Mikhaylova, V. T., and D. V. Markov. "An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections." Journal of Histochemistry & Cytochemistry 42, no. 12 (December 1994): 1643–49. http://dx.doi.org/10.1177/42.12.7983365.

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Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff-like reagent. Conditions for obtaining specific and sensitive Feulgen-like staining are determined.

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Gawlik, Anna, George Lee, Michael D. Feldman, Guangjing Zhu, Robert W. Veltri, and Anant Madabhushi. "Computer extracted nuclear features from tumor and benign regions of Feulgen and H&E images to help predict recurrence in prostate cancer patients following radical prostatectomy." Journal of Clinical Oncology 35, no. 15_suppl (May 20, 2017): e16556-e16556. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.e16556.

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e16556 Background: Following radical prostatectomy, around 30% of prostate cancer (PCa) patients experience biochemical recurrence (BCR). H&E highlights nuclear morphology and Feulgen reflects nuclear DNA content, a feature linked to PCa presence and aggressiveness. In this work we sought to explore whether computer extracted measurements of tumor morphology and tumor adjacent benign regions on H&E and Feulgen tissue images could predict BCR. Methods: We used 108 patients (59 BCR and 49 non-recurrence (NR)) and each patient had 242 QH features calculated from both the tumor and benign region of stained TMA core images. Feature selection was performed on a training set (30 BCR, 24 NR) to select the 10 most discriminating tumor and tumor adjacent benign features of each stain. A random forest classifier was trained with features so identified and validated on a test set (29 BCR, 25 NR) to predict BCR. Predictions were displayed using Kaplan-Meier analysis and area under the ROC curve (AUC). Results: The most discriminating feature from the tumor regions of the H&E stain was Fourier descriptors of nuclear shape and from the Feulgen stain was texture intensity while from the benign regions it was invariant moments of nuclear shape and texture contrast energy. Combining the significant features from tumor and tumor adjacent benign regions from H&E and Feulgen resulted in the highest accuracy and a statistically significant difference (p < 0.05) via a log-rank test (Table 1). Gleason score did not show statistically significant differences and had the lowest AUC. Conclusions: Combining nuclear morphology and DNA related features of the tumor and tumor adjacent benign regions enabled accurate prediction of BCR. With additional multi-site validation, the combined H&E + Feulgen classifier could allow better risk stratification and post-surgical patient management. [Table: see text]

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Dissertations / Theses on the topic "Feulgen"

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Zamani, Neviaty Putri. "Effects of environmental stress on cell division and other cellular parameters of zooxanthellae in the tropical symbiotic anemone Heteractis malu, Haddon and Shackleton." Thesis, University of Newcastle Upon Tyne, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294899.

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Gawlik, Anna S. "Computer Extracted Nuclear Morphologic Features from Tumor and Benign Regions of H&E and Feulgen Stained Pathology Images Predict Biochemical Recurrence and Metastasis in Prostate Cancer Patients Post-Surgery." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1498721871812207.

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Futterleib, Alexandre. "Acurácia das técnicas de Papanicolaou por citomorfologia e de Feulgen por citometria digital no diagnóstico de atipias em exames citopatológicos da mucosa oral." Pontifícia Universidade Católica do Rio Grande do Sul, 2007. http://hdl.handle.net/10923/377.

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The aim of the present study was to compare the accuracy of the methods of Papanicolaou with cytomorphology and of Feulgen with digital cytometry in the detection of cellular atypias in cytopathologic examinations of the oral mucosa. The sample comprised 68 patients with lesions as indication for biopsy, and were distributed based on clinical diagnosis as follows: 25 squamous carcinomas, one erythroplakia, three fibromas, 23 fibroepithelial hyperplasias, 14 leukoplakias and nine cases of lichen planus. The lesions were submitted to exfoliative cytology by liquid-based system, and were immediately biopsied after collection of the cytologic specimen. The cytologic specimens were processed by the methods of Papanicolaou and of Feulgen, and the histopathologic specimens by the technique of paraffin embedding and staining with hematoxylin and eosin, which totaled 225 slides, 75 for each method. The slides processed by the Papanicolaou method were submitted to cytomorphologic analysis using a light microscope and classified as positive or negative for cellular atypia. In the Feulgen method, the analysis was carried out by digital cytometry, calculating the degree of DNA ploidy. The histopathologic specimens constituted the gold standard from which were calculated the indices of sensitivity, specificity, positive predictive value, negative predictive value and accuracy for the two cytopathologic methods. The Papanicolaou method had a sensitivity of 79. 4% and specificity of 92. 1%, while for the Feulgen these indices were respectively 96. 8% and 53. 1%. The indices of positive and negative predictive value were respectively 90% and 83. 3% for the Papanicolaou and 66. 7% and 94. 4% for the Feulgen. The accuracy was 86. 1% for the Papanicolaou and 74. 6% for the Feulgen. If the leukoplakias were excluded from the evaluation, the Papanicolaou method increased in sensitivity (96. 2%) without differing significantly for the Feulgen in this respect. If all the premalignant lesions were excluded from the evaluation, the two cytopathologic methods exhibited 100% sensitivity. The results allowed us to conclude the following. (1) The methods of Feulgen combined with digital cytometry and of Papanicolaou with cytomorphology exihibit a similar accuracy in the detection of atypias in cytopathologic examinations of the oral mucosa (p=0. 093). (2) the Papanicolaou method combined with cytomorphologic analysis has a positive predictive value (p=0. 023) and specificity (p<0. 001) superior to those for Feulgen, while the latter has greater sensitivity (p=0. 037). The methods did not differ significantly with regard to the negative predictive value (p=0. 251). (3) In the Feulgen method, the cytometric nuclear variables integrated optical density, area and diameter are capable of differentiating positive and negative lesions for atypia in cytopathologic examinations of the oral mucosa. (4) The difference in sensitivity and specificity suggests that the two cytopathologic methods should be applied in combination.
A presente pesquisa teve por objetivo comparar a acurácia dos métodos de Papanicolaou por citomorfologia e de Feulgen por citometria digital na detecção de atipias celulares em exames citopatológicos da mucosa oral. A amostra foi constituída por 68 pacientes portadores de lesões com indicação de biópsia assim distribuídas de acordo com o diagnóstico clínico: 25 carcinomas espinocelulares, uma eritroplasia, três fibromas, 23 hiperplasias fibroepiteliais, 14 leucoplasias e nove casos de líquen plano. As lesões foram submetidas à citologia esfoliativa em meio líquido e, imediatamente após a coleta citológica, foram biopsiadas. As amostras citológicas foram processadas pelos métodos de Papanicolaou e de Feulgen, e as amostras histopatológicas, pela técnica da parafina e coradas com hematoxilina e eosina, o que totalizou 225 lâminas, 75 para cada método. As lâminas processadas pelo método de Papanicolaou foram submetidas à análise citomorfológica em microscópio ótico e classificadas em positivas e negativas para atipia celular. No método de Feulgen, a análise foi feita por citometria digital, calculando-se o grau de ploidia do DNA. As amostras histopatológicas constituíram o padrão-ouro, a partir do qual foram calculados os índices de sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e acurácia para ambos os métodos citopatológicos. O método de Papanicolaou teve sensibilidade de 79,4% e especificidade de 92,1%, enquanto para o Feulgen esses índices foram, respectivamente, 96,8% e 53,1%.Os índices de valor preditivo positivo e valor preditivo negativo foram, respectivamente, de 90% e 83,3% para o Papanicolaou e de 66,7% e 94,4% para o Feulgen. A acurácia de Papanicolaou foi de 86,1% e de Feulgen 74,6%. Ao serem excluídas as leucoplasias da análise, o Papanicolaou sofreu incremento de sensibilidade (96,2%) sem diferir significativamente do Feulgen neste quesito. Ao serem excluídas todas as lesões cancerizáveis da análise, os dois métodos citopatológicos exibiram 100% de sensibilidade. Os resultados permitiram concluir que: (1) os métodos de Feulgen associado à citometria digital e de Papanicolaou por citomorfologia exibem acurácia semelhante na detecção de atipias em exames citopatológicos da mucosa oral (p=0,093); (2) o método de Papanicolaou associado à análise citomorfológica tem valor preditivo positivo (p=0,023) e especificidade (p<0,001) superiores aos do Feulgen, enquanto este exibe maior sensibilidade (p=0,037). Os métodos não diferem significativamente no quesito valor preditivo negativo (p=0,251); (3) no método de Feulgen, as variáveis citométricas nucleares densidade ótica integrada, área e diâmetro são capazes de diferenciar lesões positivas e negativas para atipia em exames citopatológicos da mucosa oral; (4) as diferenças de sensibilidade e especificidade sugerem que ambos os métodos citopatológicos sejam aplicados de forma combinada.

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Wong, Alvaro Yat Set. "´´Evaluación de la compatibilidad de tinciones no fluorescentes de Diffquik, Giemsa, Fastblast y de Feulgen con el Bioensayo Cometa en el ADN espermático humano´´." Bachelor's thesis, Universidad Ricardo Palma, 2016. http://cybertesis.urp.edu.pe/handle/urp/826.

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La fertilidad masculina puede ser medida mediante un espermatograma convencional, sin embargo este examen no incluye la valoración de la integridad del ADN espermático. Esta variable ha sido correlacionada con las tasas de fertilización, viabilidad y desarrollo del embrión, convirtiéndose en una herramienta de importancia clínica tanto para los programas de reproducción animal como los tratamientos de fertilidad asistida. El bioensayo Cometa es capaz de determinar de una manera exacta el valor de la integridad del ADN espermático, lamentablemente este examen no es de uso rutinario por su elevado costo de implementación ya que utiliza microscopia especializada y tinciones fluorescentes para evidenciar la migración del ADN. El objetivo de esta investigación fue evaluar la compatibilidad de las tinciones no fluorescentes Diffquik, Giemsa, de Feulgen y FastBlast en el bioensayo Cometa usando un método visual y automatizado. Se utilizaron 15 eyaculados previamente seleccionados de acuerdo al manual OMS 2010, para luego ser capacitados en búsqueda de homogeneidad adecuada para la experimentación. Cada muestra fue expuesta a una gradiente de Peróxido de hidrogeno (0, 10, 30,60 y 100 mM) por 1 hora a 4°C para luego evaluar el coeficiente de daño mediante el método visual y porcentaje de ADN en la cola mediante el método automatizado. Las pendientes de la regresión lineal en el método visual indican que los valores obtenidos por la tinción control SybrGreen (m=3,69) difieren con Giemsa (m=3,45) y Diffquik (m=2,57). En el método automatizado de igual manera SybrGreen (m=0.83), Giemsa (m=0,79) y Diffquik (m=0,77). Sin embargo SybrGreen es 1,06 veces más efectivo que Giemsa en el visual y 1,05 veces en el automatizado, sugiriendo una compatibilidad con el bioensayo cometa. De igual manera SybrGreen es 1,07 veces más efectivo que Diffquik en el visual y 1,44 veces en el automatizado, concluyendo una compatibilidad solo en el método visual.Male fertility can be measured by a conventional semen analysis, however, this examination does not include the assessment of sperm DNA integrity. This variable has been correlated with fertilization rates, embryo viability and development, becoming a tool of clinical importance for both animal breeding programs and assisted fertility treatments. Comet bioassay is able to determine an exact way the value of sperm DNA integrity, unfortunately this test is not routinely used because of its high cost of implementation because it uses specialized microscopy and fluorescent dyes to demonstrate DNA migration. The objective of this research was to evaluate the compatibility of non-fluorescent dyes Diffquik, Giemsa, Feulgen and Comet FastBlast in the bioassay using a visual and automated method. 15 ejaculates were used previously manually selected according to WHO 2010 and then be trained in finding adequate homogeneity for experimentation. Each sample was exposed to a hydrogen peroxide gradient (0, 10, 30,60 and 100 mM) for 1 hour at 4 ° C and then assess the damage coefficient by visual method and percentage of DNA in the tail by automated method. The slopes of the linear regressions on the visual method indicate that the values obtained by the SybrGreen Control staining (m = 3.69) differ with Giemsa (m = 3.45) and Diffquik (m = 2.57). In the same way automated method SybrGreen (m = 0.83), Giemsa (m = 0.79) and Diffquik (m = 0.77). However SybrGreen is 1.06 times more effective than Giemsa visual and 1.05 times in the automated, suggesting a comet support bioassay. Similarly SybrGreen is 1.07 times more effective than Diffquik visual and 1.44 times in the automated, concluding compatibility only in the visual method. Keywords:

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Puech, Magali. "Standardisation de la mesure du contenu nucléaire en ADN par microscopie quantitative." Université Joseph Fourier (Grenoble), 1996. http://www.theses.fr/1996GRE19007.

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Silva, Brito Lima Jacqueline. "Quantificação de DNA em células com neoplasias intraepiteliais de graus I e II do colo uterino pela reação de feulgen em indivíduos do Nordeste brasileiro." Universidade Federal de Pernambuco, 2001. https://repositorio.ufpe.br/handle/123456789/2166.

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A presente pesquisa tem como objetivo detectar variações de DNA nos núcleos das células marcadas pela Reação Histoquímica de Feulgen, em indivíduos com diagnóstico de Neoplasias Intraepiteliais Cervicais de Graus I e II (NIC I e II) do colo do útero. A microcitometria foi realizada em lâminas preparadas provenientes de hospitais estaduais e universitário, submetidas à Reação Histoquímica de Feulgen, sendo analisadas no sistema Image Lab para a mensuração e absorbância. Os resultados encontrados entre as células neoplásicas dos graus I (NIC I) e II (NIC II) demonstraram que o método é sensível e eficiente na ajuda da quantificação entre as diferentes classificações, podendo, desta forma, auxiliar no diagnóstico das lesões do colo do útero em seu estágio inicial

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Futterleib, Alexandre. "Acur?cia das t?cnicas de Papanicolaou por citomorfologia e de Feulgen por citometria digital no diagn?stico de atipias em exames citopatol?gicos da mucosa oral." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2007. http://tede2.pucrs.br/tede2/handle/tede/965.

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A presente pesquisa teve por objetivo comparar a acur?cia dos m?todos de Papanicolaou por citomorfologia e de Feulgen por citometria digital na detec??o de atipias celulares em exames citopatol?gicos da mucosa oral. A amostra foi constitu?da por 68 pacientes portadores de les?es com indica??o de bi?psia assim distribu?das de acordo com o diagn?stico cl?nico: 25 carcinomas espinocelulares, uma eritroplasia, tr?s fibromas, 23 hiperplasias fibroepiteliais, 14 leucoplasias e nove casos de l?quen plano. As les?es foram submetidas ? citologia esfoliativa em meio l?quido e, imediatamente ap?s a coleta citol?gica, foram biopsiadas. As amostras citol?gicas foram processadas pelos m?todos de Papanicolaou e de Feulgen, e as amostras histopatol?gicas, pela t?cnica da parafina e coradas com hematoxilina e eosina, o que totalizou 225 l?minas, 75 para cada m?todo. As l?minas processadas pelo m?todo de Papanicolaou foram submetidas ? an?lise citomorfol?gica em microsc?pio ?tico e classificadas em positivas e negativas para atipia celular. No m?todo de Feulgen, a an?lise foi feita por citometria digital, calculando-se o grau de ploidia do DNA. As amostras histopatol?gicas constitu?ram o padr?o-ouro, a partir do qual foram calculados os ?ndices de sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e acur?cia para ambos os m?todos citopatol?gicos. O m?todo de Papanicolaou teve sensibilidade de 79,4% e especificidade de 92,1%, enquanto para o Feulgen esses ?ndices foram, respectivamente, 96,8% e 53,1%. Os ?ndices de valor preditivo positivo e valor preditivo negativo foram, respectivamente, de 90% e 83,3% para o Papanicolaou e de 66,7% e 94,4% para o Feulgen. A acur?cia de Papanicolaou foi de 86,1% e de Feulgen 74,6%. Ao serem exclu?das as leucoplasias da an?lise, o Papanicolaou sofreu incremento de sensibilidade (96,2%) sem diferir significativamente do Feulgen neste quesito. Ao serem exclu?das todas as les?es canceriz?veis da an?lise, os dois m?todos citopatol?gicos exibiram 100% de sensibilidade. Os resultados permitiram concluir que: (1) os m?todos de Feulgen associado ? citometria digital e de Papanicolaou por citomorfologia exibem acur?cia semelhante na detec??o de atipias em exames citopatol?gicos da mucosa oral (p=0,093); (2) o m?todo de Papanicolaou associado ? an?lise citomorfol?gica tem valor preditivo positivo (p=0,023) e especificidade (p<0,001) superiores aos do Feulgen, enquanto este exibe maior sensibilidade (p=0,037). Os m?todos n?o diferem significativamente no quesito valor preditivo negativo (p=0,251); (3) no m?todo de Feulgen, as vari?veis citom?tricas nucleares densidade ?tica integrada, ?rea e di?metro s?o capazes de diferenciar les?es positivas e negativas para atipia em exames citopatol?gicos da mucosa oral; (4) as diferen?as de sensibilidade e especificidade sugerem que ambos os m?todos citopatol?gicos sejam aplicados de forma combinada.

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Aagaard, Sunniva Margrethe Due. "Reticulate Evolution in Diphasiastrum (Lycopodiaceae)." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-99584.

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Kim, Andreas Verfasser], Peter A. [Akademischer Betreuer] Feulner, Martin [Akademischer Betreuer] Zacharias, and Reinhard [Akademischer Betreuer] [Kienberger. "Attosecond time-resolved photoemission from solid samples / Andreas Kim. Betreuer: Peter A. Feulner. Gutachter: Peter A. Feulner ; Martin Zacharias ; Reinhard Kienberger." München : Universitätsbibliothek der TU München, 2015. http://d-nb.info/1077605498/34.

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Feulner, Johannes [Verfasser], and Joachim [Akademischer Betreuer] Hornegger. "Machine Learning Methods in Computed Tomography Image Analysis / Johannes Feulner. Betreuer: Joachim Hornegger." Erlangen : Universitätsbibliothek der Universität Erlangen-Nürnberg, 2012. http://d-nb.info/1022931482/34.

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Books on the topic "Feulgen"

1

Edwards, Lee. Leading the way: The story of Ed Feulner and the Heritage Foundation. New York: Crown Forum, 2013.

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Virelles, Patrick. Un puma feule au fond de ma mémoire: Roman. Bruxelles: Labor, 2004.

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Book chapters on the topic "Feulgen"

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Wick, Mark R. "Feulgen, Robert (1884–1955)." In Encyclopedia of Pathology, 175–77. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-41995-4_800.

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Palchikova, Irina G., Elena A. Ivankina, Valery F. Semeshin, Leonid V. Omelyanchuk, Igor F. Zhimulev, and Eugeny S. Smirnov. "DNA Feulgen Cytophotometry and Chromatin Diminution." In International Multidisciplinary Microscopy Congress, 233–39. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-04639-6_33.

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Piętka, Bogusław D., and Annamonika Dulewicz. "Detecting Overlapped Nuclei Regions in the Feulgen-Stained Cytological Smears." In Proceedings of the 8th International Conference on Computer Recognition Systems CORES 2013, 621–28. Heidelberg: Springer International Publishing, 2013. http://dx.doi.org/10.1007/978-3-319-00969-8_61.

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Kalinowska, Kamila, Junyi Chen, and Thomas Dresselhaus. "Imaging of Embryo Sac and Early Seed Development in Maize after Feulgen Staining." In Methods in Molecular Biology, 191–203. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0342-0_14.

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Hofstädter, F., G. Jakse, and R. Delgado. "Mikroskopisch gezielte DNS-Feulgen-Zytophotometrie zur Standardisierung der Diagnose „Dysplasie“ und „Carcinoma in situ“ des Urothels." In Experimentelle Urologie, 398–402. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70524-3_55.

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"Feulgen, Robert (1884–1955)." In Encyclopedia of Parasitology, 995. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-43978-4_1189.

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Bolis, Laise Maria, Melissa Postal, and Daniela Copetti Santos. "AVALIAÇÃO DA CITOTOXICIDADE E DA GENOTOXICIDADE DA ÁGUA DOS RIOS FIÚZA E JACUÍ-MIRIM USANDO BIOENSAIO COM ALLIUM CEPA L." In Meio ambiente e sociedade: análises, diálogos e conflitos ambientais, 147–60. Editora Amplla, 2020. http://dx.doi.org/10.51859/amplla.mas078.1120-10.

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Os rios Fiúza e Jacuí-Mirim são afetados em praticamente toda a sua extensão no território de Santa Bárbara do Sul/RS por atividades agrícolas e pecuárias. O presente estudo analisou os possíveis efeitos citotóxicos e genotóxicos provocados pela água destes rios no trajeto pelo território do município usando bioensaio com Allium cepa L. As amostras foram obtidas em quatro pontos de coleta em cada rio. Para cada amostra foram colocados bulbos de cebola em triplicata para crescimento das raízes. As raízes meristemáticas foram coletadas após sete dias de exposição aos tratamentos. Para tratamento controle negativo foi utilizado água fornecida pelo abastecimento público e para controle positivo o herbicida glifosato diluído nas concentrações 0,5 e 1,0%/L. As lâminas foram preparadas através da reação de Feulgen e reativo de Schiff, coradas com orceína acética a 1% e aquecidas próximo ao fogo para melhor visualização das células. Foram analisadas no mínimo cem células por lâmina. Não foram verificadas diferença de crescimento e desenvolvimento das raízes expostas aos tratamentos com água dos rios em relação ao tratamento controle negativo, além de não serem verificados efeitos genotóxicos causados pela exposição das raízes a estas águas. Para o controle positivo não foi possível analisar o efeito genotóxico, uma vez que as raízes de Allium cepa L. não se desenvolveram no nível de concentração a que foram expostas, fator que evidencia o efeito citotóxico do herbicida glifosato.

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Conference papers on the topic "Feulgen"

1

Friedrich, David, Matthias Brozio, André Bell, Stefan Biesterfeld, Alfred Böcking, and Til Aach. "Nucleus Fingerprinting for the unique identification of Feulgen-stained Nuclei." In SPIE Medical Imaging, edited by Bram van Ginneken and Carol L. Novak. SPIE, 2012. http://dx.doi.org/10.1117/12.911304.

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Antonio Buschetto Macarini, Luiz, Aldo Von Wangenheim, Felipe Perozzo Daltoé, Alexandre Sherlley Casimiro Onofre, Fabiana Botelho de Miranda Onofre, and Marcelo Ricardo Stemmer. "Towards a Complete Pipeline for Segmenting Nuclei in Feulgen-Stained Images." In Computer on the Beach. Itajaí: Universidade do Vale do Itajaí, 2020. http://dx.doi.org/10.14210/cotb.v11n1.p169-175.

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Cervical cancer is the second most common cancer type in womenaround the world. In some countries, due to non-existent or inadequatescreening, it is often detected at late stages, making standardtreatment options often absent or unaffordable. It is a deadlydisease that could benefit from early detection approaches. It isusually done by cytological exams which consist of visually inspectingthe nuclei searching for morphological alteration. Since itis done by humans, naturally, some subjectivity is introduced. Computationalmethods could be used to reduce this, where the firststage of the process would be the nuclei segmentation. In this context,we present a complete pipeline for the segmentation of nucleiin Feulgen-stained images using Convolutional Neural Networks.Here we show the entire process of segmentation, since the collectionof the samples, passing through pre-processing, training thenetwork, post-processing and results evaluation. We achieved anoverall IoU of 0.78, showing the affordability of the approach of nucleisegmentation on Feulgen-stained images. The code is availablein: https://github.com/luizbuschetto/feulgen_nuclei_segmentation

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Agarwal, Nitin, Yiting Xie, Florence W. Patten, Anthony P. Reeves, and Eric J. Seibel. "DNA ploidy measure of Feulgen-stained cancer cells using three-dimensional image cytometry." In 2014 Health Innovations and POCT. IEEE, 2014. http://dx.doi.org/10.1109/hic.2014.7038861.

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4

Levine, R. F., and P. K. Shoff. "STIMULATED MEGAKARYOCYTES ARE FOUND IN CHILDHOOD ITP BUT NOT IN ADULT ITP." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644584.

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ITP is thought to be caused primarily by peripheral platelet destruction, but recent work has suggested that platelet production may also be impaired. Although the clinical course in children usually differs from that in adults, no distinctions have been established with regard to marrow characteristics. To evaluate megakaryocyte (mega) responses in this disease we examined mega size, ploidy, maturation and morphology in 8 children and in 8 adults with ITP and in 8 "normal" marrows (4 children, 4 adults). Marrows were prepared by a buffy coat wedge smear or by a cover slip squash method. Control values differed according to the type of marrow smear used. From 100-300 Feulgen-stained megas were examined in each specimen, as previously described. Wright-stained material was also examined. With the squash method megas from normal children and adults had similar characteristics. The megas of each child with acute ITP showed marked increases in size (volumes were 4X normal), ploidy (as high as 1024 N; medians were 64N, or 2 doublings higher than normals), and maturation stage (86% mature forms vs 43%). In contrast, none of the marrows of adults with acute or chronic ITP (1 with mild, 4 moderate, and 3 with severe thrombocytopenias) showed any stimulation of megas. Overall, their megas were normal in size, ploidy and maturation. Occasional dissociation of mega ploidy and maturation was seen, but not enough to alter the profiles of any one parameter. There were no obvious or suggestive signs of "damage" to the megas of children or adults with ITP. In conclusion, the megas of childhood ITP showed a pattern of marked stimulation of size, ploidy and maturation, as seen in animals injected with antiplatelet serum. The failure of adult marrows to response in these parameters to thrombocytopenia may be pathogenetically related to the chronicity of adult ITP.

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Hoffman, R., B. J. Roth, G. W. Sledge, J. Straneva, and J. Brandt. "ANALYSIS OF PHORBOL ESTER STIMULATED HUMAN MEGAKARYOCYTE DEVELOPMENT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642951.

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The events that occur during the terminal maturation of human megakaryocytes are poorly characterized. To examine these events, a recently characterized human megakaryocytic cell line (EST-IU, Cancer Res. 46: 2155-2159, 1986) was exposed to 12-0-tetradecanoyl-phorbol-13-acetate (TPA), as well as 2 non-transforming phorbol esters (4 alpha phorbol and 4 beta phorbol 12 alpha, 13 alpha diacetate) at the identical concentrations. Morphologic changes, including cellular attachment to untreated plastic or glass, occurred within 4 hrs of treatment with TPA. Treatment of EST-IU cells with either of the 2 non-transforming phorbols (4-alpha phorbol, or 4-beta phorbol, 12-beta, 13-alpha diacetate) failed to change morphology, DNA content, or expression of surface membrane glycoproteins or alpha-granule constituents when compared to control cells. TPA treatment resulted, however, in j^rofound changes in adherence to plastic by the EST-IU cells, with an obvious dose-response relationship. At a 5 × 10-8 M TPA, cellular attachment was noted as early as 4 hours following treatment, agd was complete by 16 hours, at which time > 95% of treated cells were attached. Following TPA treatment at 5 × 10-8 M, a number of morphologic changes occurred, including marked cellular flattening, the appearance of extensive cytoplasmic budding, and the development of numerous filopodia. Cells treated with either of the non-transforming phorbols as assessed by propidium iodide staining and flow cytometric analysis failed to exhibit a change in ploidy, although TPA reproducibly altered this parameter of megakaryocyte development. Cells treated with 10-9 M TPA have approximately the same proportion of cells in the 4N and 8N peaks as control cells. Following exposure to 10-9 M and 10-8 M TPA, there was an apparent shift of cells out of the 4N peak to 8N and 16N levels, and even the appearance of a small percentage of 32N cells. The DNA content of TPA-treated cells was also assessed by Feulgen staining and microdensitome try. Those cells (5%) which failed to adhere following TPA treatment were analyzed separately, and showed a very different ploidy distribution than the adherent cell population. Over 85% of adherent cells have a ploidy > 16N, with some cells attaining the 128N level. Treatment of cells with either of the 2 non-transforming phorbols failed to affect the expression of Factor V, Factor VIIIrRAg, beta-throraboglobulin, fibrinogen, or platelet glycoproteins. Cells treated with 5 × 10-8 M TPA similarly do not significantly increse the expression of Factor V, fibrinogen, or beta-thromoglobulin over that observed in control cells. The expression of both Factor VIIIrRAg and platelet glycoproteins however, increase in TPA-treated cells. A similar increase in the expression of platelet glycoprotein Ilb/IIIA using the mouse monoclonal C17 was also observed. Those cells that express the highest levels of Factor VIII:RAg and platelet glycoproteins following phorbol treatment also demonstrated the highest ploidy levels and also are the largest cells as measured by forward angle light scatter during flow cytometry.These studies indicate that TPA treatment of EST-IU cells initiates a cascade of events characterized by cellular adherence, increases in cell size and DNA content, and enhanced expression of platelet glycoproteins and Factor VIIIrRAg. These events appear to occur in concert and closely resemble information that is available concerning maturation of normal rodent and human megakaryocytes. Although it is important to emphasize that EST-IU cells are leukemic and thus intrinsically different from normal human megakaryocytes, their availability and dynamic responses to TPA will provide an appropriate cellular model with which to study megakaryocyte maturation.

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Spakovszky, Z. S., J. B. Gertz, O. P. Sharma, J. D. Paduano, A. H. Epstein, and E. M. Greitzer. "Influence of Compressor Deterioration on Engine Dynamic Behavior and Transient Stall-Margin." In ASME 1999 International Gas Turbine and Aeroengine Congress and Exhibition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/99-gt-439.

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This paper presents an experimental and analytical investigation of compressor stability assessment during engine transient operation. A 2-dimensional, linear, compressible, state-space analysis of stall-inception (Feulner et al. (1996)) was modified to account for engine transients and deterioration, with the latter modeled as increased tip-clearance and flow blockage. Experiments were performed on large commercial aircraft engines in both undeteriorated and deteriorated states. Unsteady measurements of pressure in these test engines during rapid accelerations revealed the growth of pre-stall disturbances, which rotate at rotor speed and at approximately half rotor speed. These disturbances are stronger in deteriorated engines. The model showed that the signal at shaft speed was the first compressible system mode, whose frequency is near shaft speed, excited by geometric nonuniformities. The computed behavior of this mode during throttle transients closely matched engine data. The signal increased in strength as stall was approached and as the engine deteriorated. This work firmly establishes the connection between observed signals in the these engines and first principles stability models.

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