Journal articles: 'Feulgen'

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Ebnet, Klaus, and D. Vestweber. "Robert Feulgen Lecture 1998." Histochemistry and Cell Biology 112, no. 1 (July 20, 1999): 1–23. http://dx.doi.org/10.1007/s004180050387.

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Rasp, E., and J. E. Dumont. "1 Robert Feulgen Lecture 1991." Progress in Histochemistry and Cytochemistry 26, no. 1-4 (January 1992): 1–29. http://dx.doi.org/10.1016/s0079-6336(11)80074-4.

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Patil, Shankargouda, and Anveeta Agarwal. "Comparison and Evaluation of Mitotic Figures in Oral Epithelial Dysplasia using Crystal Violet and Feulgen Stain." Journal of Contemporary Dental Practice 15, no. 3 (2014): 273–77. http://dx.doi.org/10.5005/jp-journals-10024-1527.

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ABSTRACT Background Routine staining procedures often pose a problem in differentiating a mitotic cell from an apoptotic cell, deteriorating the reliability of histology grading. Although various new methods have been recommended for identifying mitotic figures (MFs) in tissues, the time factor and cost makes them less feasible. Thus, an attempt was made to evaluate the efficacy of crystal violet and Feulgen reaction in identifying MFs and also to see for any variation in the number of MFs in various grades of Epithelial dysplasia. Objectives: 1. Using crystal violet and Feulgen stain in the identification and counting of MFs on diagnosed cases of epithelial dysplasia and thereby to evaluate their efficacy. 2. To evaluate the variation in the number of MFs in various grades of epithelial dysplasia. Materials and methods The study sample includes retrieval of 30 formalin fixed paraffin embedded tissue sections diagnosed for different grades of epithelial dysplasia (WHO grading system, 2005) from the archives, Department of Oral Pathology, MSRDC, Bengaluru. Ten tissue sections each of mild, moderate and severe epithelial dysplasia were stained with H&E, Feulgen and 1% crystal violet stains and the number of MFs were counted. Five cases of cervical carcinoma were taken as control. Stained sections were compared, and data obtained was statistically analyzed using the Kruskal-Wallis test. Results A significant increase in the number of MFs (p = 0.02) was observed in Feulgen stained sections as compared to H&E stain. Conclusion Feulgen stain can be considered as a simple, reliable, cost-effective and reproducible method of staining MFs. How to cite this article Rao RS, Patil S, Agarwal A. Comparison and Evaluation of Mitotic Figures in Oral Epithelial Dysplasia using Crystal Violet and Feulgen Stain. J Contemp Dent Pract 2014;15(3):273-277.

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Lin, P., and D. C. Allison. "Measurement of DNA content and of tritiated thymidine and bromodeoxyuridine incorporation by the same cells." Journal of Histochemistry & Cytochemistry 41, no. 9 (September 1993): 1435–39. http://dx.doi.org/10.1177/41.9.8354883.

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We tested a method of measuring DNA content (Feulgen) and tritiated thymidine ([3H]-T) and bromodeoxyuridine (BrdU) incorporation by the same cell. Initial experiments showed that Feulgen hydrolysis denatured the DNA of fixed cells sufficiently to allow detection of incorporated BrdU with monoclonal antibodies. MCa-11 cells were then double-labeled with [3H]-T and BrdU, placed on slides, and Feulgen stained. Next, absorption cytometry was performed to measure the DNA content of randomly selected cells. Feulgen staining and the development and removal of either the [3H]-T or the BrdU grains after DNA measurements did not interfere with subsequent detection of the grains from the other label, and BrdU and [3H]-T can be used reliably in combination for identification of S-phase cells. This method may eventually allow the use of microscope-based image analysis to selectively measure the DNA contents and the BrdU/[3H]-T labeling of non-transformed stromal and cancer cells in solid tumors, thereby providing new insights into the growth kinetics of these heterogeneous cell populations.

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Foucrier, J., J. P. Rigaut, and D. Pechinot. "A combined AgNOR-Feulgen staining technique." Journal of Histochemistry & Cytochemistry 38, no. 11 (November 1990): 1591–97. http://dx.doi.org/10.1177/38.11.1698851.

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We describe a new staining technique (H-Ag-S) which allows observation and counting of active nucleolus organizer regions (NORs) and evaluation of the amount of DNA in the same cell nucleus. The procedure consists of combining a modified AgNOR staining method with the Feulgen reaction. A sequential procedure is proposed, based on the determination of optimal staining conditions. The technique, which was designed to allow studies of correlations between the transcriptional activity of rDNA genes and the cell ploidy, was primarily developed for rat liver smears. It should be applicable to most biological preparations, but the optimal conditions might be variable.

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Chieco, Pasquale, and M. Derenzini. "The Feulgen reaction 75 years on." Histochemistry and Cell Biology 111, no. 5 (May 20, 1999): 345–58. http://dx.doi.org/10.1007/s004180050367.

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Schulte, E., and D. Wittekind. "Standardization of the Feulgen-Schiff technique." Histochemistry 91, no. 4 (1989): 321–31. http://dx.doi.org/10.1007/bf00493008.

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Kesarkar, Kashmira, Avinash Tamgadge, Treville Peirera, Sandhya Tamgadge, Swati Gotmare, and Pooja Kamat. "Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains." Sultan Qaboos University Medical Journal [SQUMJ] 18, no. 2 (September 9, 2018): 149. http://dx.doi.org/10.18295/squmj.2018.18.02.005.

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Objectives: The objectives of this study were to quantitatively estimate the number of mitotic figures (MFs) and evaluate the cellular and nuclear features of various histological grades of oral squamous cell carcinoma (OSCC) using Feulgen and 1% crystal violet stains. Methods: This case-control study took place at the Dr D. Y. Patil Dental College & Hospital in Mumbai, Maharashtra, India, between June and December 2016. A total of 51 samples were retrieved from the hospital archives. Of these, 15 well-differentiated, 15 moderately-differentiated and six poorly-differentiated OSCC samples formed the case group while 15 samples of normal gingival mucosa constituted the control group. Each sample was dyed using Feulgen and 1% crystal violet stains and the mitotic count, nuclear area (NA), cellular area (CA), nuclear perimeter (NP), cellular perimeter (CP) and nuclear-to-cytoplasmic (N/C) ratio was calculated using computeraided morphometry techniques. Results: The number of MFs visible per field was significantly higher in Feulgen-stained sections as compared to those stained with crystal violet (P = 0.050). In addition, the NA, NP, CA and CP values and N/C ratios of samples in the experimental group increased significantly in accordance with an increase in OSCC grade (P <0.001). Conclusion: The Feulgen stain is more reliable than 1% crystal violet in terms of the selective staining of MFs. Moreover, the findings of this study indicate that computer-based morphometric analysis is an effective tool for differentiating between various grades of OSCC.Keywords: Crystal Violet; Feulgen Stain; Mitotic Index; Image Cytometry; Squamous Cell Carcinoma; Oral Cancers.

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Mikhaylova, V. T., and D. V. Markov. "An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections." Journal of Histochemistry & Cytochemistry 42, no. 12 (December 1994): 1643–49. http://dx.doi.org/10.1177/42.12.7983365.

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Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff-like reagent. Conditions for obtaining specific and sensitive Feulgen-like staining are determined.

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Gawlik, Anna, George Lee, Michael D. Feldman, Guangjing Zhu, Robert W. Veltri, and Anant Madabhushi. "Computer extracted nuclear features from tumor and benign regions of Feulgen and H&E images to help predict recurrence in prostate cancer patients following radical prostatectomy." Journal of Clinical Oncology 35, no. 15_suppl (May 20, 2017): e16556-e16556. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.e16556.

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e16556 Background: Following radical prostatectomy, around 30% of prostate cancer (PCa) patients experience biochemical recurrence (BCR). H&E highlights nuclear morphology and Feulgen reflects nuclear DNA content, a feature linked to PCa presence and aggressiveness. In this work we sought to explore whether computer extracted measurements of tumor morphology and tumor adjacent benign regions on H&E and Feulgen tissue images could predict BCR. Methods: We used 108 patients (59 BCR and 49 non-recurrence (NR)) and each patient had 242 QH features calculated from both the tumor and benign region of stained TMA core images. Feature selection was performed on a training set (30 BCR, 24 NR) to select the 10 most discriminating tumor and tumor adjacent benign features of each stain. A random forest classifier was trained with features so identified and validated on a test set (29 BCR, 25 NR) to predict BCR. Predictions were displayed using Kaplan-Meier analysis and area under the ROC curve (AUC). Results: The most discriminating feature from the tumor regions of the H&E stain was Fourier descriptors of nuclear shape and from the Feulgen stain was texture intensity while from the benign regions it was invariant moments of nuclear shape and texture contrast energy. Combining the significant features from tumor and tumor adjacent benign regions from H&E and Feulgen resulted in the highest accuracy and a statistically significant difference (p < 0.05) via a log-rank test (Table 1). Gleason score did not show statistically significant differences and had the lowest AUC. Conclusions: Combining nuclear morphology and DNA related features of the tumor and tumor adjacent benign regions enabled accurate prediction of BCR. With additional multi-site validation, the combined H&E + Feulgen classifier could allow better risk stratification and post-surgical patient management. [Table: see text]

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Temsch, Eva M., and Johann Greilhuber. "Genome size variation in Arachis hypogaea and A. monticola re-evaluated." Genome 43, no. 3 (June 1, 2000): 449–51. http://dx.doi.org/10.1139/g99-130.

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Genome size variation within species is a frequently reported, but still a controversial problem. In the present study, we re-evaluated recently published Feulgen densitometric data on genome size and its infraspecific variation in Arachis hypogaea, and also conducted measurements in one accession of its wild relative A. monticola. The methods applied were propidium iodide flow cytometry and Feulgen densitometry using Pisum sativum as an internal standard. The 2C DNA contents previously published cannot be confirmed, but values obtained in this study are about half as large. Additionally, we could not reproduce the previously reported 1.15-fold variation within A. hypogaea; our data indicate genome size stability between respective accessions of this species. Based on 8.84 pg (2C) for Pisum sativum the DNA amounts (2C) were: 5.914 pg in A. hypogaea, and 5.979 pg in A. monticola.Key words: Arachis, genome size, flow cytometry, Feulgen densitometry.

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Maria, Silvya Stuchi, Benedicto de Campos Vidal, and Maria Luiza S. Mello. "Image analysis of DNA fragmentation and loss in V79 cells under apoptosis." Genetics and Molecular Biology 23, no. 1 (March 2000): 109–12. http://dx.doi.org/10.1590/s1415-47572000000100020.

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Nuclear image analysis of Feulgen-stained V79 fibroblasts after three days in culture was used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as the Feulgen-DNA content, degree of chromatin condensation and nuclear areas, in association with visual morphology. The fibroblasts were initially plated at a density of 10(5) cells/ml and incubated under optimal culture conditions without subculturing. Following confluency, the cells underwent contact inhibition apoptosis. Image analysis revealed three nuclear phenotypes which were defined in terms of their morphological characteristics and levels of chromatin condensation. A decrease in the amount of Feulgen-DNA was detected in apoptotic cells and in cells suspected of undergoing apoptosis. This decrease was assumed to indicate DNA loss. Image analysis procedures may therefore provide a useful tool for discriminating cells in the early stages of apoptosis.

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Greilhuber, Johann. "Severely distorted Feulgen-DNA amounts in Pinus (Coniferophytina) after nonadditive fixations as a result of meristematic self-tanning with vacuole contents." Canadian Journal of Genetics and Cytology 28, no. 3 (June 1, 1986): 409–15. http://dx.doi.org/10.1139/g86-060.

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Highly divergent nuclear DNA amounts were obtained in Pinus mugo and Pinus cembra when meristematic tissue from root tips was fixed either with neutral formaldehyde or various nonadditive agents as methanol – acetic acid, ethanol – acetic acid, alcohols alone, Carnoy's fluid, acetone, or was directly hydrolyzed with 5 M HCl. After formaldehyde fixation, the 1C values in P. mugo and P. cembra amount to 20.16 and 24.16 pg, respectively, when calibrated against Allium cepa as internal standard, but 1C values after application of nonadditive fixatives are strongly reduced to 25–41% of the former values. This phenomenon is explained by the observation that in Pinus a large fraction of the meristematic cells contains a considerably large vacuome, whose content (probably condensed tannins) becomes immobilized after formaldehyde fixation and further on does not interfere with the Feulgen reaction, whereas after nonadditive fixations the vacuole contents extravasate and strongly tan the whole meristem and especially the nuclei. The Feulgen reaction is impaired. The tint and absorbance spectra are different in pine and Allium cepa nuclei. Pinus Feulgen-DNA values obtained after fixations other than formaldehyde must be regarded as highly distorted from the true genomic DNA content. Self-tanning as a source of methodical error in DNA content determinations by cytochemical techniques may be widespread because of the frequent occurrence of tannins in the plant kingdom.Key words: Feulgen reaction, DNA contents, tannins, Pinus, conifers.

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Benedum, J., and Matthias Meusch. "Robert Feulgen (1884-1955) - some biographical thoughts." Histochemistry and Cell Biology 111, no. 5 (May 20, 1999): 337–43. http://dx.doi.org/10.1007/s004180050366.

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NOKKALA, SEPPO. "Staining the centrioles with Feulgen and Giemsa." Hereditas 99, no. 1 (June 28, 2008): 161–63. http://dx.doi.org/10.1111/j.1601-5223.1983.tb00743.x.

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LIMA-de-FARIA, A. "THE FEULGEN TEST APPLIED TO CENTROMERIC CHROMOMERES." Hereditas 36, no. 1 (July 9, 2010): 60–74. http://dx.doi.org/10.1111/j.1601-5223.1950.tb03363.x.

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Hardie, David C., T. Ryan Gregory, and Paul D. N. Hebert. "From Pixels to Picograms." Journal of Histochemistry & Cytochemistry 50, no. 6 (June 2002): 735–49. http://dx.doi.org/10.1177/002215540205000601.

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The study of genome size variation is important from a number of practical and theoretical perspectives. For example, the long-standing “C-value enigma” relating to the more than 200,000-fold range in eukaryotic genome sizes is best studied from a broad comparative standpoint. Genome size data are also required in detailed analyses of genome structure and evolution. The choice of future genome sequencing projects will be dependent on knowledge regarding the sizes of genomes to be sequenced, and so on. To date, genome size data have been acquired primarily by Feulgen microdensitometry or flow cytometry. Each has several advantages but also important limitations. In this review, we provide a practical guide to the new technique of Feulgen image analysis densitometry. The review is designed for those interested in genome size measurements but not extensively experienced in histochemistry, densitometry, or microscopy. Therefore, relevant historical and technical background information is included. For easy reference, we provide recipes for required reagents, guidelines for cell staining, and a checklist of steps for successful image analysis. We hope that the accuracy, rapidity, and cost-effectiveness of Feulgen image analysis demonstrated here will stimulate further surveys of genome sizes in a variety of taxa.

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Greilhuber, J., Monika Baranyi, and J. Greilhuber. "Feulgen Densitometry: Importance of a Stringent Hydrolysis Regime." Plant Biology 1, no. 5 (September 1999): 538–40. http://dx.doi.org/10.1111/j.1438-8677.1999.tb00780.x.

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&NA;. "Robert Feulgen Prize 1991—Applications Now Being Accepted." American Journal of Surgical Pathology 14, no. 7 (July 1990): 702. http://dx.doi.org/10.1097/00000478-199007000-00019.

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Symonds, Daniel A., David P. Johnson, and Clifford R. Wheeless. "Feulgen cytometry in simultaneous endometrial and ovarian carcinoma." Cancer 61, no. 12 (June 15, 1988): 2511–16. http://dx.doi.org/10.1002/1097-0142(19880615)61:12<2511::aid-cncr2820611220>3.0.co;2-y.

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Kasten, Frederick H. "Robert Feulgen and his histochemical reaction for DNA." Biotechnic & Histochemistry 78, no. 1 (January 2003): 45–49. http://dx.doi.org/10.1080/10520290312120009.

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Bauer, Henrik C. F., and Andris Kreicbergs. "Feulgen DNA stainability of bone tumors after demineralization." Cytometry 8, no. 6 (November 1987): 590–94. http://dx.doi.org/10.1002/cyto.990080610.

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Kerr, A. R., B. Å. Risberg, D. A. Sirois, K. E. Fleisher, J. Mo, and P. G. Sack. "Comparison of two DNA-specific staining protocols, Feulgen-Thionin and Feulgen-PAS for DNA ploidy measurements of oral epithelial cells." Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 102, no. 3 (September 2006): 339. http://dx.doi.org/10.1016/j.tripleo.2006.06.044.

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Melo-Junior, Mario Ribeiro, Marcos Cezar Feitosa de Paula Machado, Jorge Luiz Silva Araújo-Filho, Vasco José Ramos Malta-Patu, and Adriana Maria Da Silva Telles. "Análise morfométrica de micronúcleos e colágeno intersticial em tumores de mama." Revista de Ciências Médicas e Biológicas 7, no. 1 (August 17, 2008): 49. http://dx.doi.org/10.9771/cmbio.v7i1.4357.

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O presente trabalho estabeleceu a densidade de micronúcleos e a distribuição do colágeno intersticial no tecido tumoral benigno e maligno de mama humana. Foram utilizadas a reação histoquímica de Feulgen e tricrômico de Masson, e a análise digital de imagens. Foram selecionados fragmentos de tecido mamário, diagnosticados como Fibroadenoma (FA), Doença fibrocística (DF) e Carcinoma Ductal Infiltrante (CDI). Para controle, foram utilizadas amostras de plástica mamária com tecido normal. A reação histoquímica foi realizada através da reação de Feulgen, para evidenciação dos micronúcleos, e, para a análise de colágeno intersticial, foi utilizada a coloração tricrômico de Masson. O número médio de micronúcleos exibiu valores semelhantes em amostra de FA (90,8 ± 4,5), DF (80,3 ± 4,0) e tecido normal (83,0 ± 4,1). Entretanto, valores significativamente menores que aqueles foram encontrados no CDI (176,5 ± 88,3). Quanto à distribuição do colágeno intersticial, constatou-se um aumento estatisticamente significante (p< 0,001) nos casos de FA (8101,4 ± 405,07) e DF (7046,78 ± 352,33), quando comparados ao tecido mamário normal (1733,13 ± 86,65) e ao CDI (2165,94 ± 108,29). Pode-se concluir que tanto o método de análise de imagem como a reação de Feulgen mostraram-se eficientes para evidenciar as alterações do núcleo celular e da matriz extracelular de tumores de mama.

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Schifino-Wittmann, Maria Teresa. "Determinação da quantidade de DNA nuclear em plantas." Ciência Rural 31, no. 5 (October 2001): 897–902. http://dx.doi.org/10.1590/s0103-84782001000500028.

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O valor C de DNA é um caráter de significado biológico fundamental e o conhecimento da quantidade de DNA nuclear de um grupo de organismos pode ser útil em vários campos da ciência. Do ponto de vista prático, a determinação da quantidade de DNA nuclear, que é mais comumente realizada por microdensitometria de Feulgen e citometria de fluxo, pode substituir a contagem de cromossomos, especialmente quando se trabalha com um número muito grande de indivíduos. A microdensitometria por Feulgen baseia-se na ligação específica do DNA a esse corante, havendo uma proporcionalidade entre a quantidade de DNA existente e a quantidade de corante que o núcleo incorporou. A citometria de fluxo envolve a análise das propriedades óticas de partículas em fluxo. Em plantas, basicamente consiste no isolamento dos núcleos, coloração destes com um fluorocromo e leitura da fluorescência emitida. As grandes vantagens desta técnica, em relação à microdensitometria de Feulgen, são a relativa facilidade e a rapidez da preparação das amostras, o grande número de núcleos que podem ser analisados, a necessidade de pequenas quantidades de tecido e a possibilidade de detecção de pequenas diferenças na quantidade de DNA. Relatos de resultados conflitantes entre determinações de diferentes autores mostram a necessidade de uma criteriosa padronização das técnicas, para que problemas metodológicos não venham a ser interpretados como eventuais diferenças reais na quantidade de DNA.

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Gregory, T. Ryan. "Genome size estimates for two important freshwater molluscs, the zebra mussel (Dreissena polymorpha) and the schistosomiasis vector snail (Biomphalaria glabrata)." Genome 46, no. 5 (October 1, 2003): 841–44. http://dx.doi.org/10.1139/g03-069.

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The haploid genome sizes of two important molluscs were assessed by Feulgen image analysis densitometry. The genome size of the zebra mussel (Dreissena polymorpha), a prolific invader of North American lakes, was estimated to be 1C = 1.70 ± 0.03 pg, and that of the freshwater snail Biomphalaria glabrata, the predominant intermediate vector of the human parasite Schistosoma mansoni, was estimated at 0.95 ± 0.01 pg. These estimates will be important in future efforts in molluscan genomics, which at present lags far behind work being carried out with vertebrate and arthropod models. B. glabrata in particular, which has one of the smallest known gastropod genomes, is recommended as a highly suitable target for future genome sequencing.Key words: densitometry, DNA content, DNA sequencing, Feulgen, image analysis, Great Lakes, invading species, molluscs, Schistosoma mansoni.

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Erlandsen, S. L., and E. M. Rasch. "The DNA content of trophozoites and cysts of Giardia lamblia by microdensitometric quantitation of Feulgen staining and examination by laser scanning confocal microscopy." Journal of Histochemistry & Cytochemistry 42, no. 11 (November 1994): 1413–16. http://dx.doi.org/10.1177/42.11.7930524.

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We investigated direct measurement of the DNA content of the parasitic intestinal flagellate Giardia lamblia through quantitation by Feulgen microspectrophotometry and also by visualization of Feulgen-stained DNA chromosomes within dividing cells by laser scanning confocal microscopy. Individual trophozoites of Giardia (binucleate) contained 0.144 +/- 0.018 pg of DNA/cell or 0.072 pg DNA/nucleus. Giardia lamblia cysts (quadranucleate) contained 0.313 +/- 0.003 pg DNA or 0.078 pg DNA/nucleus. The genome size (C) value per nucleus ranged between 6.5-7.1 x 10(7) BP for trophozoites and cysts, respectively. Confocal microscopic examination of Giardia trophozoites undergoing binary fission revealed five chromosome-like bodies within each nucleus. Further information about genome size and DNA content within different Giardia species may help to clarify the pivotal role of these primitive eukaryotic cells in evolutionary development.

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Schneller, Janet, Elisabeth Eppich, Ellen Greenebaum, Flora Elequin, Andrew Sherman, Robert Wersto, and Leopold G. Koss. "Flow cytometry and feulgen cytophotometry in evaluation of effusions." Cancer 59, no. 7 (April 1, 1987): 1307–13. http://dx.doi.org/10.1002/1097-0142(19870401)59:7<1307::aid-cncr2820590713>3.0.co;2-q.

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Braselton, James P., Michael J. Wilkinson, and Stephen A. Clulow. "Feulgen Staining of Intact Plant Tissues for Confocal Microscopy." Biotechnic & Histochemistry 71, no. 2 (January 1996): 84–87. http://dx.doi.org/10.3109/10520299609117139.

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ANAMTHAWAT-JONSSON, KESARA, LADAWAN ATIPANUMPAI, PETER M. A. TIGERSTEDT, and THORSTEINN TOMASSON. "The Feulgen-Giemsa method for chromosomes of Betula species." Hereditas 104, no. 2 (February 14, 2008): 321–22. http://dx.doi.org/10.1111/j.1601-5223.1986.tb00547.x.

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Mello, Maria Luiza S., and Benedicto de Campos Vidal. "The Feulgen reaction: A brief review and new perspectives." Acta Histochemica 119, no. 6 (July 2017): 603–9. http://dx.doi.org/10.1016/j.acthis.2017.07.002.

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Coq, C. Le, C. Guervin, M. Esclapez, and J. Moret. "Méthode d'étude des quantités d'ADN métaphasique après écrasement de méristèmes radiculaires." Genome 35, no. 4 (August 1, 1992): 706–8. http://dx.doi.org/10.1139/g92-108.

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A method is described for standardized preparation of meristematic root cells treated with colchicine for cytophotométrie analysis of metaphase DNA. Deoxyribonucleic acid has been stained by the Feulgen reaction prior to crushing of the cells.Key words: cytophotometry, Ornithogalum, nuclear DNA content.

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Mello, Maria Luiza S., and Jose Russo. "Image analysis of Feulgen-stained c-H-ras-transformed NIH/3T3 cells." Biochemistry and Cell Biology 68, no. 7-8 (July 1, 1990): 1026–31. http://dx.doi.org/10.1139/o90-151.

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Feulgen–DNA content, nuclear phenotypes, and levels of chromatin condensation were evaluated by image analysis in NIH/3T3 cells transformed with the c-H-ras oncogene of T24 cells. Three nuclear phenotypes, differing from those of untransformed control cells and defined in terms of patterns of chromatin condensation, were demonstrated microspectrophotometrically for the tumor cells. Polyploidy could only be observed in nuclei with extensive and deeply stained areas covered with condensed chromatin, i.e., only in a small fraction of the tumor cell nuclear population. The increased chromatin condensation that appeared with cell transformation affected the euchromatin zones. The image analysis provided data that, compared with those obtained in other situations involving cell transformation, could be relevant to the understanding of changes in chromatin supraorganization related to tumorigenesis and to tumor cell diagnosis.Key words: cell transformation, c-H-ras oncogene, NIH/3T3 cells, image analysis, Feulgen staining.

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Kiss, R., I. Salmon, I. Camby, S. Gras, and J. L. Pasteels. "Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction." Journal of Histochemistry & Cytochemistry 41, no. 6 (June 1993): 935–45. http://dx.doi.org/10.1177/41.6.8315284.

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We investigated the parameters that could affect the cytophotometric analysis of cell nuclei stained by the Feulgen reaction. These parameters included: the hydrolysis temperature (in the normal "room temperature" range); the composition of the Schiff's reagent; the speed of centrifugation of the cell suspensions; the mode of preservation [air-drying or ethanol-formalin-acetic acid (EFA) fixation]; the fixation time; the pronase digestion time; and the concentration of pronase used to obtain cell suspensions from archival (formalin-fixed, paraffin-embedded) materials. Relatively homogeneous material was studied: the MXT mouse mammary adenocarcinoma growing in vivo as tumors with both small and hyperchromatic cell nuclei and in vitro as monolayers with larger and less hyperchromatic cell nuclei. The results of these investigations demonstrate the necessity for the precise definition of a protocol for such procedures as sampling, fixation, and staining of cell nuclei if computerized cell image analyses are to be objective and reproducible. For present purposes this protocol differs depending on whether fresh or archival material is studied. For fresh tissue the protocol is immersion of the sample in EFA within 10 sec, fixation for 30 min, and staining by the Feulgen reaction in which hydrolysis is performed with 6 N HCl at 24 degrees C for 60 min. For archival tissue, the protocol becomes fixation in formol (or EFA), embedding, sectioning at 80 microns, digestion with 0.05% pronase for 2 hr, centrifugation at 1200 x g on glass slides, and staining by the Feulgen reaction in which hydrolysis is performed with 6 N HCl for 60 min at 24 degrees C.

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35

Fominaya, A., and N. Jouve. "C-banding at meiosis as a means of analyzing cytogenetic structure in wheat." Canadian Journal of Genetics and Cytology 27, no. 6 (December 1, 1985): 689–96. http://dx.doi.org/10.1139/g85-103.

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The characterization of reciprocal interchanges between chromosomes of wheat in the progeny of the backcross ((T. aestivum H-53 × S. cereale) × T. aestivum H-53) using Feulgen and C-banding staining techniques has been attempted. The translocated chromosomes were studied in detail in three lines using monosomic analysis. In the F1 of the crosses between these lines and the 21 monosomic lines of 'Chinese Spring' a total of five interchanges were identified. Using a Giemsa C-banding procedure it was possible to confirm the identity of the interchange chromosome pairs that were previously identified by monosomic analysis. Moreover, a total of 12 new interchanges were characterized or identified. C-banding also facilitated the identification of the arms involved in translocations and permitted the quantitative analysis of each multivalent in multiple interchange heterozygotes. The comparative availability of Feulgen and C-banding in studies of cytogenetic structure in wheat is discussed.Key words: C-banding, meiosis, heterochromatin, translocations, wheat, Triticum.

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36

Greilhuber, J., and I. Ebert. "Genome size variation in Pisum sativum." Genome 37, no. 4 (August 1, 1994): 646–55. http://dx.doi.org/10.1139/g94-092.

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Pisum sativum L. is one of the plant species where infraspecific genome size variation, up to 1.29-fold between cultivars, has been reported. The present investigation deals with a Feulgen cytophotometric analysis of this phenomenon in 25 wild accessions, landraces, and cultivars of widely different geographic origin. Differences between accessions were maximally 1.054-fold in single experiments but proved to be nonreproducible upon repeated measurements. Seedlings of the same accession often differed significantly, up to 1.056-fold, but values from root and shoot tips in one individual were not significantly correlated, indicating the absence of true genome size variation between plants. Upon calibration against Allium cepa a 1C value of 4.42 pg is estimated for Pisum sativum. Altogether the data suggest that, contrary to the divergence in the literature data and recent reports on DNA content variation, the pea has a stable genome size.Key words: Pisum sativum L., genome size variation, Feulgen cytophotometry.

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37

Oppedal, B. R., A. Glomstein, and A. Zetterberg. "Feulgen DNA Values in Wilms' Tumour in Relation to Prognosis." Pathology - Research and Practice 183, no. 6 (November 1988): 756–60. http://dx.doi.org/10.1016/s0344-0338(88)80061-x.

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38

Salih, Magdi M., Basem Elesawy, Salman M. Al-Ayli, Awad A. Al-Otaibi, Fares M. Al-Thobiti, and Mazen Almehmadi. "Demonstration of Deoxyribonucleic Acid in Colorectal Cancer Using Feulgen reaction." Annals of Cancer Research and Therapy 28, no. 2 (July 9, 2020): 88–92. http://dx.doi.org/10.4993/acrt.28.88.

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39

Voglmayr, Hermann, and Johann Greilhuber. "Genome Size Determination in Peronosporales (Oomycota) by Feulgen Image Analysis." Fungal Genetics and Biology 25, no. 3 (December 1998): 181–95. http://dx.doi.org/10.1006/fgbi.1998.1097.

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40

Whitaker, B. P., W. W. Stigliano, F. L. Carson, and J. A. Lynn. "Thionin Feulgen Stain for DNA (Ploidy) Quantitation by Image Analysis." Journal of Histotechnology 16, no. 2 (June 1993): 113–16. http://dx.doi.org/10.1179/his.1993.16.2.113.

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41

MELLO, M., and A. CHAMBERS. "Image analysis of feulgen-stained -transformed NIH 3T3 cell clones." Cell Biology International Reports 14 (September 1990): 174. http://dx.doi.org/10.1016/0309-1651(90)90795-z.

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Hömmö, Leena M., and Eliisa M. Särkilahti. "A method of counting chromosomes of hardwood trees using root tips and young leaves." Canadian Journal of Forest Research 16, no. 2 (April 1, 1986): 401–3. http://dx.doi.org/10.1139/x86-070.

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Double staining with Feulgen and Giemsa was used for root tips and young leaves and it allowed counts of the mitotic chromosomes of Betulapapyrifera Marsh, and Populustremula L. The following chromosome numbers were easily determined: B. papyrifera, 2n = 70, 2n = 84, and 2n = 140; P. tremula, 2n = 38 and 2n = 57.

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43

Coutinho, Libério França, Juliano Batista do Amaral, Érico Brito dos Santos, Elizabeth Ferreira Martinez, Victor Angelo M. Montalli, José Luiz Cintra Junqueira, Vera Cavalcanti de Araújo, and Marcelo Henrique Napimoga. "Presence of Cells in Fresh-Frozen Allogeneic Bone Grafts from Different Tissue Banks." Brazilian Dental Journal 28, no. 2 (April 2017): 152–57. http://dx.doi.org/10.1590/0103-6440201701206.

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Bone replacement materials have been widely used to reconstruct atrophic jawbones. Based on previous reports demonstrating the presence of viable cells in bone blocks even after processing by musculoskeletal tissue banks for orthopedic use, the aim of this study was to evaluate the presence of cells in bone blocks from three Brazilian tissue banks for maxillary reconstructions. All samples were processed by the respective tissue banks, according to the guidelines of the Brazilian National Sanitary Surveillance Agency. Three samples were removed from each block for subsequent histological processing and stained using hematoxylin & eosin. Further evaluation included section staining by the Feulgen method and ultrastructural analysis using scanning electron microscopy (SEM). Light microscopy images from all bone samples showed presence of osteocyte-like cells in all groups and intense Feulgen staining, demonstrating presence of DNA in bone even after tissue processing. The ultrastructural analysis showed red blood cells in lacunae within the bone tissue. In conclusion, despite bone tissue processing by the musculoskeletal tissue banks, cells may be found within the bone used for allogeneic grafts.

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Almeida, Hipolito de Oliveira, Elizabeth Martins, José Umberto Franciscon, Vicente de Paula Antunes Teixeira, Alfredo José Afonso Barbosa, Helenice Gobbi, and Marlene Antonia dos Reis. "Características das células parasitadas pelo Trypanosoma cruzi na parede da veia central das supra-renais de Chagásicos crônicos." Revista da Sociedade Brasileira de Medicina Tropical 19, no. 4 (December 1986): 227–31. http://dx.doi.org/10.1590/s0037-86821986000400005.

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Foram analisados os aspectos das células musculares parasitadas pelo Trypanosoma cruzi, na veia da supra-renal de chagásicos crônicos, através de exame ao microscópio óptico de lâminas coradas pela hematoxilina-eosina (HE), PAS, Feulgen e peroxidase-antiperoxidase (PAP) para antigenos do T. cruzi. Além das modificações nucleares descritas anteriormente, os leiomiócitos parasitados exibem alterações citoplasmáticas que podem ser vistas mesmo em células que albergam poucos parasitas. As formas amastigotas geralmente estão envoltas por halo claro e o citoplasma restante adquire aspecto granuloso ou reticular, basófilopelo HE, sendo sempre PAS e Feulgen negativos. Estes dados sugerem que o material basófilo no citoplasma deve ser RNA ribossômico. A periferia dos ninhos que mostram uma "membrana" com reação do PAP para antigenos do T. cruzi fortemente positiva, poderia ser devida a reação cruzada de material celular rechaçado para a periferia ou a difusão de antigenos do T. cruzi e sua adsorção à periferia celular. O material citoplasmático PAP positivo poderia resultar de artefato, de reação imunocitoquimica cruzada, de antigenos tripanossomóticos difundidos ou de antigenos tripanossoma-simile resultantes de interações entre o leiomiócito e o parasita.

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Ceccarelli, M., and P. G. Cionini. "Tissue-specific nuclear repatterning in plant cells." Genome 36, no. 6 (December 1, 1993): 1092–98. http://dx.doi.org/10.1139/g93-145.

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Tissue-specific nuclear repatternings, consisting of changes in the number and size of the chromocenters, were observed by analyzing, in Feulgen squashes and sections, different tissues of several plant species, particularly of Ionopsidium savianum. Nuclear repatternings occur mainly near the base of the meristems. They are due to associations of chromosomes at their heterochromatic regions. This was confirmed by the results of cytophotometric measurements, showing the same contents of both Feulgen/DNA and heterochromatin in nuclei with a different number of chromocenters. These data also showed that chromosome association does not occur in endoreduplicating or endoreduplicated cells. Autoradiographic results after [3H]thymidine treatments indicated that DNA synthesis does not occur in nuclei with extensive chromosome association. A highly significant, positive correlation was found between the number of chromocenters in each nucleus and the amount of RNA synthesis as indicated by [3H]uridine incorporation. It is suggested that chromosome association plays some role in the regulation of the functional activity of the nucleus and in tissue differentiation.Key words: functional regulation, heterochromatin, nuclear repatterning, plant cell nucleus, tissue differentiation.

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Nicol, Theresa L., and Grover M. Hutchins. "Intraairway Feulgen-Positive Bodies in Hyaline Membrane Disease of the Newborn." Pediatric Pathology & Laboratory Medicine 15, no. 1 (January 1995): 99–107. http://dx.doi.org/10.3109/15513819509026942.

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47

Kuo, Sow-Hsong, Jin-Chuan Sheu, Ding-Shinn Chen, Juei-Low Sung, Chi-Chung Lin, and Hey-Chi Hsu. "DNA clonal heterogeneity of hepatocellular carcinoma demonstrated by Feulgen-DNA analysis." Liver 7, no. 6 (December 10, 2008): 359–63. http://dx.doi.org/10.1111/j.1600-0676.1987.tb00367.x.

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48

D'Amato, Gianni. "Different Types of Heterochromatin in Plant Chromosomes Investigated by Feulgen Banding." Caryologia 39, no. 2 (January 1986): 123–29. http://dx.doi.org/10.1080/00087114.1986.10797773.

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LEE, S., T. CHUNG, and R. MILBRADT. "Feulgen-Nuklearreaktion in Spermatozoenköpfen von jugendlichen Ratten nach Amethopterin-Langzeit-Behandlung." Andrologia 6, no. 3 (April 24, 2009): 269–76. http://dx.doi.org/10.1111/j.1439-0272.1974.tb01214.x.

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50

FALISTOCCO, EGIZIA, and MARCO PICCIRILLI. "The Basic Karyotype of Lotus tenuis C-banding and Feulgen Studies." Annals of Botany 63, no. 4 (April 1989): 401–4. http://dx.doi.org/10.1093/oxfordjournals.aob.a087759.

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Journal articles on the topic 'Feulgen'

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