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Journal articles on the topic 'Fibrolytic'

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1

Lynch, J. P., D. Prema, J. D. Van Hamme, J. S. Church, and K. A. Beauchemin. "Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant." Canadian Journal of Animal Science 94, no. 4 (2014): 697–704. http://dx.doi.org/10.4141/cjas-2014-086.

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Lynch, J. P., Prema, D., Van Hamme, J. D., Church, J. S. and Beauchemin, K. A. 2014. Fiber degradability, chemical composition and conservation characteristics of alfalfa haylage ensiled with exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant. Can. J. Anim. Sci. 94: 697–704. This study investigated the effects of two fibrolytic enzyme products, applied at baling alone or in combination with a ferulic acid esterase-producing bacterial additive, on the ensilage dynamics, chemical composition and digestibility of alfalfa haylage. Five replicate wrapped bales were produce
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Matshogo, Tumisang Ben, Caven Mguvane Mnisi, and Victor Mlambo. "Effect of Pre-Treating Dietary Green Seaweed with Proteolytic and Fibrolytic Enzymes on Physiological and Meat Quality Parameters of Broiler Chickens." Foods 10, no. 8 (2021): 1862. http://dx.doi.org/10.3390/foods10081862.

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The use of seaweeds as nutraceuticals in chicken diets is limited by high fibre levels and low protein digestibility. Therefore, we tested the effect of pre-treating dietary seaweed (Ulva sp.) with a combination of protease and fibrolytic enzymes on physiological and meat quality parameters of Cobb 500 broilers. Five dietary treatments were formulated by including untreated (T1); fibrolytic (12 g/kg) enzyme-treated (T2); fibrolytic (12 g/kg) and protease (5 g/kg) enzyme-treated (T3); fibrolytic (12 g/kg) and protease (10 g/kg) enzyme-treated (T4); fibrolytic (12 g/kg) and protease (15 g/kg) en
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Collinet, Axelle, Pauline Grimm, Samy Julliand, and Véronique Julliand. "Sequential Modulation of the Equine Fecal Microbiota and Fibrolytic Capacity Following Two Consecutive Abrupt Dietary Changes and Bacterial Supplementation." Animals 11, no. 5 (2021): 1278. http://dx.doi.org/10.3390/ani11051278.

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In horses, abrupt changes from high-fiber (HF) to high-starch (HS) diets can affect the cecal and colonic microbiota. This study investigated modifications and recovery of fecal microbiota after two consecutive abrupt dietary changes. Twelve horses fed HF for 2 weeks were changed to HS for 5 days then returned to HF for 7 weeks. Six received lactic acid bacteria supplementation. Bacterial population diversity, structure, and activity, especially fibrolysis, were assessed to obtain an overview of alteration in hindgut microbiota. Two days after the abrupt change from HF to HS, the findings in f
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4

Montgomery, L. "Isolation of human colonic fibrolytic bacteria." Letters in Applied Microbiology 6, no. 3 (1988): 55–57. http://dx.doi.org/10.1111/j.1472-765x.1988.tb01214.x.

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5

Freiria, Lucien Bissi da, Joanis Tilemahos Zervoudakis, Nelcino Francisco de Paula, et al. "Kinetic parameters of ruminal degradation in vitro with combinations of exogenous enzymes in diets of production systems simulated." Revista Brasileira de Saúde e Produção Animal 19, no. 1 (2018): 69–82. http://dx.doi.org/10.1590/s1519-99402018000100007.

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SUMMARY This study evaluated the effects of eight combinations of three exogenous enzymes with fibrolytic activity (FIB), amylolytic activity (AMZ), and proteolytic activity (PRO) on the accumulated gas production (PG) and kinetic parameters in simulated fermentations in two diet production systems using the in vitro gas production technique. In experiment 1, ruminal fluid was obtained from two Santa Ines sheep, cannulated in the rumen, kept in a feedlot and fed a diet containing a roughage:concentrate ratio of 20:80. In experiment 2, the ruminal fluid was obtained from two Santa Ines sheep, c
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Dong, Y., H. D. Bae, T. A. McAllister, G. W. Mathison та K. J. Cheng. "Effects of exogenous fibrolytic enzymes, α-bromoethanesulfonate and monensin on fermentation in a rumen simulation (RUSITEC) system". Canadian Journal of Animal Science 79, № 4 (1999): 491–98. http://dx.doi.org/10.4141/a99-024.

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The effects of exogenous fibrolytic enzymes, α-bromoethanesulfonate (BES) and monensin on fermentation and digestibility of grass hay were examined using two rumen simulation technique (RUSITEC) devices, each equipped with eight 820-mL fermenters with a liquid dilution rate of 0.73 d−1. Grass hay (10 g d−1) was fed either untreated or pre-treated with 0.2 g each of cellulase and xylanase, and in combination with no chemical or daily dosing of 20.5 µmol monensin, 20.5 µmol BES or 41 µmol BES. Pretreatment of hay with the fibrolytic enzymes increased (P < 0.05) organic matter (OM), cellulose
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Lemos, Maikon Figueredo, Alexandro Pereira Andrade, Pedro Henrique Ferreira da Silva, et al. "Nutritional value, fermentation losses and aerobic stability of elephant grass (Pennisetum purpureum Schum.) silage treated with exogenous fibrolytic enzymes." Acta Scientiarum. Animal Sciences 42 (June 8, 2020): e48272. http://dx.doi.org/10.4025/actascianimsci.v42i1.48272.

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The aim of this study was to evaluate nutritional value, fermentation losses, and aerobic stability of elephant grass silage (Pennisetum purpureum Schum.) treated with exogenous fibrolytic enzymes. The experiment was conducted in a completely randomized design with four replicates (experimental silos) and five levels of fibrolytic enzymes (0, 1.5, 3.0, 4.5 and 6.0%). For this, the elephant grass was ensiled at 70 days of age in plastic buckets with 20L capacity. Silos were opened 60 days after sealing. Analyses were made for chemical composition, in vitro dry matter digestibility (IVDMD), effl
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8

Mendoza, Germán D., Octavio Loera-Corral, Fernando X. Plata-Pérez, Pedro A. Hernández-García, and Mónica Ramírez-Mella. "Considerations on the Use of Exogenous Fibrolytic Enzymes to Improve Forage Utilization." Scientific World Journal 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/247437.

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Digestion of cell wall fractions of forage in the rumen is incomplete due to the complex links which limit their degradation. It is therefore necessary to find options to optimize the use of forages in ruminant production systems. One alternative is to use exogenous enzymes. Exogenous fibrolytic enzymes are of fungal or bacterial origin and increase nutrient availability from the cell wall, which consists of three fractions in different proportions depending on the species of forage: digestible, potentially digestible, and indigestible. The response to addition of exogenous enzymes varies with
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9

Koike, Satoshi, and Yasuo Kobayashi. "Fibrolytic Rumen Bacteria: Their Ecology and Functions." Asian-Australasian Journal of Animal Sciences 22, no. 1 (2009): 131–38. http://dx.doi.org/10.5713/ajas.2009.r.01.

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10

Ribeiro, G. O., R. J. Gruninger, A. Badhan, and T. A. McAllister. "Mining the rumen for fibrolytic feed enzymes." Animal Frontiers 6, no. 2 (2016): 20–26. http://dx.doi.org/10.2527/af.2016-0019.

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11

Nsereko, V. L., K. A. Beauchemin, D. P. Morgavi, et al. "Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows." Canadian Journal of Microbiology 48, no. 1 (2002): 14–20. http://dx.doi.org/10.1139/w01-131.

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The effects of supplementing a dairy cow diet with incremental levels of a fibrolytic enzyme preparation (preparation B) from Trichoderma longibrachiatum on the rumen microbial population were investigated. Two cows fitted with rumen cannulae were each fed a diet containing barley-based concentrate (52%), maize silage (29%), and chopped alfalfa hay (19%), supplemented with 0, 1, 2, 5, or 10 L of preparation B per tonne of dry matter (DM). Preparation B stimulated numbers of total viable bacteria in a quadratic manner (P < 0.05), to approximately 230, 330, 390, and 250% at 1, 2, 5, and 10 L·
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12

Dash, Jeevan R., Tapas K. Sar, Rinku Buragohain, Indranil Samanta, and Rahul Nanotkar. "Evaluation of Fibrolytic Effect of Bauhinia purpurea L." Clinical Cancer Drugs 6, no. 1 (2019): 21–32. http://dx.doi.org/10.2174/2212697x06666190131155256.

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Background: Mammary gland tissue (left half) revealed the presence of excess proliferation of fibrous tissue with disorganization of alveolar structures and the right half showed extensive fibrous tissue proliferation of mammary gland following intramammary inoculation of 2000 c.f.u. of Staphylococcus aureus. However, oral dosing of Bauhinia variegata L. bark powder at 6 gm/kg for 7 days and 3 gm/kg for another 7 days exhibited reduction of fibrous tissue in chronic mastitis. Objective: The fibrolytic effect of one week oral dosing of Bauhinia purpurea L. bark powder was studied in chronic mas
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13

Bestion, Eloïne, Zuzana Macek Jilkova, Jean-Louis Mège та ін. "GNS561 acts as a potent anti-fibrotic and pro-fibrolytic agent in liver fibrosis through TGF-β1 inhibition". Therapeutic Advances in Chronic Disease 11 (січень 2020): 204062232094204. http://dx.doi.org/10.1177/2040622320942042.

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Background: Hepatic fibrosis is the result of chronic liver injury that can progress to cirrhosis and lead to liver failure. Nevertheless, there are no anti-fibrotic drugs licensed for human use. Here, we investigated the anti-fibrotic activity of GNS561, a new lysosomotropic molecule with high liver tropism. Methods: The anti-fibrotic effect of GNS561 was determined in vitro using LX-2 hepatic stellate cells (HSCs) and primary human HSCs by studying cell viability, activity of caspases 3/7, autophagic flux, cathepsin maturation and activity, HSC activation and transforming growth factor-β1 (T
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14

Sun, X. Z., K. N. Joblin, I. G. Andrew, S. O. Hoskin, and P. J. Harris. "Degradation of forage chicory by ruminal fibrolytic bacteria." Journal of Applied Microbiology 105, no. 5 (2008): 1286–97. http://dx.doi.org/10.1111/j.1365-2672.2008.03861.x.

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15

Chu, Agustín Rascón, Carmen Armida Contreras Vergara, Ciria Guadalupe Figueroa Soto, Tania Elisa González Soto, Martín Esqueda Valle, and José Alfonso Sánchez Villegas. "FIBROLYTIC ACTIVITY OF PODAXIS PISTILLARIS FUNGUS IN SUBMERGED CULTURE." Biotecnia 21, no. 1 (2019): 120–26. http://dx.doi.org/10.18633/biotecnia.v21i1.874.

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Podaxis pistillaris is a fungus commonly found in most desert areas worldwide. The oval shaped peridium, the rigid woody stipe, plus a 10-15 μm spore size stand out among its morphological features. Even though this fungus is used for human consumption and for several traditional remedies, a lack of knowledge regarding its fibrolytic enzymatic system still prevails. This fungus was collected from the central region of the Sonoran desert (29° 07.23´ 97” LN and 110° 53.58´ 02” LW, 238 masl). In order to study its enzymatic system on common fibers, P. pistillaris was grown in a specific submerged
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16

FARENZENA, R., G. V. KOZLOSKI, M. P. MEZZOMO, and A. C. FLUCK. "Forage degradability, rumen bacterial adherence and fibrolytic enzyme activityin vitro: effect of pH or glucose concentration." Journal of Agricultural Science 152, no. 2 (2013): 325–32. http://dx.doi.org/10.1017/s0021859613000427.

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SUMMARYA set of independent assays were conducted to assess the effects of either pH or glucose concentration on forage degradation, bacterial adherence and on fibrolytic enzyme activityin vitro. For measuring degradation and bacterial adherence, ryegrass (Lolium multiflorum) and bermudagrass (Cynodon dactylon) samples were incubatedin vitrofor 24 h in the medium at different pH (5·5, 6·0, 6·5 or 7·0) or with different initial glucose concentrations (0, 1000, 2000 and 3000 mg/l). For fibrolytic enzyme activity evaluation, forage samples were incubatedin situand the extracted enzymes were incub
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17

Popov, Yury, Deanna Y. Sverdlov, K. Ramakrishnan Bhaskar, et al. "Macrophage-mediated phagocytosis of apoptotic cholangiocytes contributes to reversal of experimental biliary fibrosis." American Journal of Physiology-Gastrointestinal and Liver Physiology 298, no. 3 (2010): G323—G334. http://dx.doi.org/10.1152/ajpgi.00394.2009.

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Studies have suggested the reversibility of liver fibrosis, but the mechanisms of fibrosis reversal are poorly understood. We investigated the possible functional link between apoptosis, macrophages, and matrix turnover in rat liver during reversal of fibrosis secondary to bile duct ligation (BDL). Biliary fibrosis was induced by BDL for 4 wk. After Roux-en-Y (RY)-bilio-jejunal-anastomosis, resolution of fibrosis was monitored for up to 12 wk by hepatic collagen content, matrix metalloproteinase (MMP) expression and activities, and fibrosis-related gene expression. MMP expression and activitie
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18

Rode, L. M., W. Z. Yang, and K. A. Beauchemin. "Fibrolytic Enzyme Supplements for Dairy Cows in Early Lactation." Journal of Dairy Science 82, no. 10 (1999): 2121–26. http://dx.doi.org/10.3168/jds.s0022-0302(99)75455-x.

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19

Padberg, K., S. Y. Weng, and D. Schuppan. "352 ADENOSINE SWITCHES (LIVER) MACROPHAGES TOWARDS A FIBROLYTIC PHENOTYPE." Journal of Hepatology 58 (April 2013): S146. http://dx.doi.org/10.1016/s0168-8278(13)60354-2.

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20

Titgemeyer, Evan C., B. J. Johnson, and John E. Shirley. "Performanceof lactating dairy cows fed yeast and fibrolytic enzymes." Kansas Agricultural Experiment Station Research Reports, no. 2 (January 1, 2004): 55–58. http://dx.doi.org/10.4148/2378-5977.3175.

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21

Józefiak, D., M. Komosa, P. Maćkowiak, et al. "Exogenous fibrolytic enzymes improve carbohydrate digestion in exercising horses." Journal of Animal and Feed Sciences 29, no. 1 (2020): 35–45. http://dx.doi.org/10.22358/jafs/118207/2020.

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22

Dai, Xin, Yaxin Zhu, Yingfeng Luo, et al. "Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen." PLoS ONE 7, no. 7 (2012): e40430. http://dx.doi.org/10.1371/journal.pone.0040430.

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23

SINGH, KRISHNA M., AJAI K. TRIPATHI, PARESH R. PANDYA, et al. "Use of Real-Time PCR Technique in Determination of Major Fibrolytic and non Fibrolytic Bacteria Present in Indian Surti Buffaloes (Bubalus bubalis)." Polish Journal of Microbiology 62, no. 2 (2013): 195–200. http://dx.doi.org/10.33073/pjm-2013-025.

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In the milk industry in India, buffalo breeds are most commonly used for milk production. Efficiency of fiber digestion in ruminants is critical for animal productivity. Bacteria play an important role in fiber digestion and utilization. Absolute quantification real-time PCR was used to quantify ten bacterial species in rumen fluid of Surti buffalo fed green fodder, dry roughage and compound concentrate mixture. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific primers. Bacterial populations showed a clear predomi
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Dagar, S. S., N. Singh, N. Goel, S. Kumar, and A. K. Puniya. "Role of anaerobic fungi in wheat straw degradation and effects of plant feed additives on rumen fermentation parameters in vitro." Beneficial Microbes 6, no. 3 (2015): 353–60. http://dx.doi.org/10.3920/bm2014.0071.

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In the present study, rumen microbial groups, i.e. total rumen microbes (TRM), total anaerobic fungi (TAF), avicel enriched bacteria (AEB) and neutral detergent fibre enriched bacteria (NEB) were evaluated for wheat straw (WS) degradability and different fermentation parameters in vitro. Highest WS degradation was shown for TRM, followed by TAF, NEB and least by AEB. Similar patterns were observed with total gas production and short chain fatty acid profiles. Overall, TAF emerged as the most potent individual microbial group. In order to enhance the fibrolytic and rumen fermentation potential
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Erdoğan, Sibel, and Murat Demirel. "Conservation Characteristics and Nutritive Value of Sunflower Silages as Affected by The Maturity Stages and Fibrolytic Enzymes." Turkish Journal of Agriculture - Food Science and Technology 4, no. 6 (2016): 464. http://dx.doi.org/10.24925/turjaf.v4i6.464-469.652.

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Silage quality and nutritive value of sunflower silages ensiled with different level of fibrolytic enzymes at blooming, milk and dough stages were investigated. Fibrolytic enzyme complex (Viscozyme® L, V2010 Sigma Novozyme) was applied 0, 1, 1.5 and 2.5 ml/kgDM at ensiling. The dry matter (DM) yield increased with each increment of the maturity stage. Dry matter (DM), ether extract (EE) and crude cellulose (CC) contents of silages increased also in each delay in harvesting, while crude protein (CP), NDF and ADF contents of silages were the highest in blooming stage. The pH was higher in sunflo
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26

Kondratovich, Lucas B., Jhones O. Sarturi, Carly A. Hoffmann, Michael A. Ballou, Sara J. Trojan, and Pedro R. B. Campanili. "Effects of dietary exogenous fibrolytic enzymes on ruminal fermentation characteristics of beef steers fed high- and low-quality growing diets1." Journal of Animal Science 97, no. 7 (2019): 3089–102. http://dx.doi.org/10.1093/jas/skz165.

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Abstract The effects of dietary pretreatment with fibrolytic enzyme-based cocktail were evaluated in 2 studies: (1) in vitro true digestibility; and (2) intake, digestibility, feeding behavior, and ruminal fermentation of beef steers fed growing diets. For the in vitro assessment, the ruminal inoculum was collected from 2 steers (BW = 543 ± 45 kg; 4-h after feeding; growing diets) and enzymes included or not (Trichoderma reesei fermentation extract; 0.75 µL/g of substrate DM). Within in vitro batches (n = 4), 12 substrates were incubated and in vitro true nutrient digestibility was evaluated.
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27

Kumar, Brishketu, and Sunil Sirohi. "Effect of isolate of ruminal fibrolytic bacterial culture supplementation on fibrolytic bacterial population and survivability of inoculated bacterial strain in lactating Murrah buffaloes." Veterinary World 5, no. 12 (2013): 14. http://dx.doi.org/10.5455/vetworld.2013.14-17.

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28

Cai, Shichun, Jiabao Li, Fen Ze Hu, et al. "Cellulosilyticum ruminicola, a Newly Described Rumen Bacterium That Possesses Redundant Fibrolytic-Protein-Encoding Genes and Degrades Lignocellulose with Multiple Carbohydrate- Borne Fibrolytic Enzymes." Applied and Environmental Microbiology 76, no. 12 (2010): 3818–24. http://dx.doi.org/10.1128/aem.03124-09.

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ABSTRACT Cellulosilyticum ruminicola H1 is a newly described bacterium isolated from yak (Bos grunniens) rumen and is characterized by its ability to grow on a variety of hemicelluloses and degrade cellulosic materials. In this study, we performed the whole-genome sequencing of C. ruminicola H1 and observed a comprehensive set of genes encoding the enzymes essential for hydrolyzing plant cell wall. The corresponding enzymatic activities were also determined in strain H1; these included endoglucanases, cellobiohydrolases, xylanases, mannanase, pectinases, and feruloyl esterases and acetyl ester
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29

Freiria, Lucien Bissi da, Joanis Tilemahos Zervoudakis, Nelcino Franciso de Paula, et al. "Do fibrolytic, proteolytic and amylolytic enzymes influence the in vitro fermentation characteristics of forage?" Semina: Ciências Agrárias 39, no. 3 (2018): 1143. http://dx.doi.org/10.5433/1679-0359.2018v39n3p1143.

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The effects of increasing doses of three exogenous enzymes preparations with fibrolytic activity (FIB - 0, 0.6, 1.2, 1.8, and 2.4 mg mL-1liquid volume incubated), amylolytic activity (AMZ - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-1liquid volume incubated), and proteolytic activity (PRO - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-1 liquid volume incubated ) on gas production (GP), kinetic parameters, and fermentation profile of Brachiaria brizantha cv. Marandu were evaluated using the in vitro gas production technique. Ruminal liquid was obtained from two rumen-cannulated Santa Inês sheep maintained on p
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30

Morgavi, D. P., K. A. Beauchemin, V. L. Nsereko, et al. "Synergy Between Ruminal Fibrolytic Enzymes and Enzymes from Trichoderma Longibrachiatum." Journal of Dairy Science 83, no. 6 (2000): 1310–21. http://dx.doi.org/10.3168/jds.s0022-0302(00)74997-6.

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31

Elwakeel, E. A., E. C. Titgemeyer, B. J. Johnson, C. K. Armendariz, and J. E. Shirley. "Fibrolytic Enzymes to Increase the Nutritive Value of Dairy Feedstuffs." Journal of Dairy Science 90, no. 11 (2007): 5226–36. http://dx.doi.org/10.3168/jds.2007-0305.

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32

Satapathy, Debasish, Tapas Dutta, and Anupam Chatterjee. "Application and Limitations of Exogenous Fibrolytic Enzymes in Animal Nutrition." International Journal of Livestock Research 8, no. 5 (2018): 21. http://dx.doi.org/10.5455/ijlr.20170926065352.

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cherla, Mo, V. A. N. Suryanarayana, and P. Kavitha. "Role of Exogenous Fibrolytic Enzymes in Ruminant Digestion - A Review." International Journal of Current Microbiology and Applied Sciences 6, no. 11 (2017): 1400–1408. http://dx.doi.org/10.20546/ijcmas.2017.611.167.

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34

Sakita, Gabriel Zanuto, Thiago Francisco Ventoso Bompadre, Dhanasekaran Dineshkumar, et al. "Fibrolytic enzymes improving in vitro rumen degradability of tropical forages." Journal of Animal Physiology and Animal Nutrition 104, no. 5 (2020): 1267–76. http://dx.doi.org/10.1111/jpn.13373.

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35

Beauchemin, KA, LM Rode, and VJH Sewalt. "Fibrolytic enzymes improve growth of steers fed forage-based diets." Annales de Zootechnie 44, Suppl. 1 (1995): 69. http://dx.doi.org/10.1051/animres:19950539.

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36

Tirado-González, Deli Nazmín, Luis Alberto Miranda-Romero, Agustín Ruíz-Flores, Sergio Ernesto Medina-Cuéllar, Rodolfo Ramírez-Valverde, and Gustavo Tirado-Estrada. "Meta-analysis: effects of exogenous fibrolytic enzymes in ruminant diets." Journal of Applied Animal Research 46, no. 1 (2017): 771–83. http://dx.doi.org/10.1080/09712119.2017.1399135.

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37

Abid, Khalil, Jihene Jabri, Yves Beckers, et al. "Influence of adding fibrolytic enzymes on the ruminal fermentation of date palm by-products." Archives Animal Breeding 62, no. 1 (2019): 1–8. http://dx.doi.org/10.5194/aab-62-1-2019.

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Abstract. This study was conducted in order to assess the influence of four doses (0, 0.5, 1, and 2 mg (g dry matter)−1 of commercial fibrolytic enzymes (MAXFIBER-I®, SHAUMANN GmbH, Wahlstedt, Germany) on in vitro fermentation of date palm (Phoenix dactylifera) by-products: date kernels, wasted dates, floral stems, and palm fronds. Rumen contents were obtained from two non-lactating Holstein cows. Enzyme supplementation to by-products was carried out 12 h prior to incubation. Compared to the control, the enzymatic supplementation quadratically increased the extent but not the gas production ra
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38

Urdaneta, A. Barrios, M. Fondevila, J. Balcells, C. Dapoza, and C. Castrillo. "In vitro microbial digestion of straw cell wall polysaccharides in response to supplementation with different sources of carbohydrates." Australian Journal of Agricultural Research 51, no. 3 (2000): 393. http://dx.doi.org/10.1071/ar99079.

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The effect of carbohydrate supplementation on microbial fibre digestion was studied in vitro, by measuring the disappearance of cell wall monosaccharides, bacterial adhesion (mmol purine bases per g residue), and total (per g residue) and bacterial (per mmol purine bases) polysaccharidase activity. Straw cell walls (CW, 0.5% w/v) were cultured in medium supplemented with (0.275% w/v) or without starch, a sugar mixture, or pectin. Supplementation with these constituents did not cause a drop in pH below 6.1, and increased all parameters investigated with the exception of bacterial polysaccharida
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Chaucheyras-Durand, Frédérique, Sébastien Masséglia, Gérard Fonty, and Evelyne Forano. "Influence of the Composition of the Cellulolytic Flora on the Development of Hydrogenotrophic Microorganisms, Hydrogen Utilization, and Methane Production in the Rumens of Gnotobiotically Reared Lambs." Applied and Environmental Microbiology 76, no. 24 (2010): 7931–37. http://dx.doi.org/10.1128/aem.01784-10.

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ABSTRACT We investigated the influence of the composition of the fibrolytic microbial community on the development and activities of hydrogen-utilizing microorganisms in the rumens of gnotobiotically reared lambs. Two groups of lambs were reared. The first group was inoculated with Fibrobacter succinogenes, a non-H2-producing species, as the main cellulolytic organism, and the second group was inoculated with Ruminococcus albus, Ruminococcus flavefaciens, and anaerobic fungi that produce hydrogen. The development of hydrogenotrophic bacterial communities, i.e., acetogens, fumarate and sulfate
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40

Nsereko, V. L., D. P. Morgavi, K. A. Beauchemin, and L. M. Rode. "Inhibition of ruminant feed enzyme polysaccharidase activities by extracts from silages." Canadian Journal of Animal Science 80, no. 3 (2000): 523–26. http://dx.doi.org/10.4141/a00-015.

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Extracts from 14 barley silages inhibited endo-1, 4-β-xylanase and α-amylase activities of a ruminant feed enzyme additive from Trichoderma longibrachiatum by 23 to 50% but had little effect on cellulase activity. The inhibitory factor(s) were <10 kDa in size and were stable to autoclaving. These observations may explain why feed enzymes are generally less effective when applied to silages than when applied to dry feeds. Key words: Silage, fibrolytic enzymes, Trichoderma, xylanase, inhibitors
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41

Colombatto, D., F. L. Mould, M. K. Bhat, and E. Owen. "Effects of incubation fluid pH and fibrolytic enzymes on the in vitro fermentation of pure substrates, assessed using the Reading Pressure Technique (RPT)." Proceedings of the British Society of Animal Science 2002 (2002): 208. http://dx.doi.org/10.1017/s1752756200008644.

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Modern feeding practices often lead to ruminal conditions being sub-optimal for fibre digestion. It has been speculated that fibrolytic enzymes, which usually show optimum activity at pH values below 6.0, may be of benefit when applied to diets of high producing animals. This study used a commercial enzyme mixture (EM), already identified as effective; to investigate its optimum pH range with respect to activity and its impact on the fermentation profiles of pure substrates, under differing pH conditions.
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42

Mir, Sayar Ahmad, M. S. Pannu, Ovais Aarif, and Nazam Khan. "In saccodegradability of wheat straw treated with urea and fibrolytic enzymes." Indian Journal of Animal Research 48, no. 1 (2014): 21. http://dx.doi.org/10.5958/j.0976-0555.48.1.005.

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43

Bowman, G. R., K. A. Beauchemin, and J. A. Shelford. "In vitro degradation of fresh substrates treated with exogenous fibrolytic enzymes." Canadian Journal of Animal Science 82, no. 4 (2002): 611–15. http://dx.doi.org/10.4141/a02-030.

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An exogenous fibrolytic enzyme product was applied to three different proportions of a total mixed ration (TMR) and fed to dairy cows. There was no enhancement of the hydrolytic capacity of rumen fluid from cows serving as inoculum donors as a result of enzyme supplementation. In vitro dry matter degradation of the TMR at 12 h of incubation was increased by 15% when enzymes were added to the concentrate portion, and by 17% when added to the premix portion of the TMR (P < 0.05), but adding enzymes to the supplement (pelleted portion of the concentrate) had no effect. Key words: Enzymes, fres
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44

Azzaz, H. H., H. A. Murad, A. M. Kholif, T. A. Morsy, A. M. Mansour, and H. M. El-Sayed. "Increasing Nutrients Bioavailability by Using Fibrolytic Enzymes in Dairy Buffaloes Feeding." Journal of Biological Sciences 13, no. 4 (2013): 234–41. http://dx.doi.org/10.3923/jbs.2013.234.241.

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45

Martin, C., L. Genestoux, and B. Michalet-Doreau. "Effect of pH on fibrolytic activity in rumen solid-adherent microorganisms." CrossRef Listing Of Deleted DOIs 43, Suppl. 1 (1994): 21s—22s. http://dx.doi.org/10.1051/rnd:19940522.

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46

Shinkai, Takumi, Ryohei Ohji, Nobuya Matsumoto, and Yasuo Kobayashi. "Fibrolytic capabilities of ruminal bacteriumFibrobacter succinogenesin relation to its phylogenetic grouping." FEMS Microbiology Letters 294, no. 2 (2009): 183–90. http://dx.doi.org/10.1111/j.1574-6968.2009.01565.x.

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47

Martin, C., L. Genestoux, and B. Michalet-Doreau. "Effect of pH on fibrolytic activity in rumen solid-adherent microorganisms." Annales de Zootechnie 43, Suppl. 1 (1994): 21s—22s. http://dx.doi.org/10.1051/animres:19940522.

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48

Liu, Je-Ruei, Bi Yu, Fu-Hwa Liu, Kuo-Joan Cheng, and Xin Zhao. "Expression of Rumen Microbial Fibrolytic Enzyme Genes in Probiotic Lactobacillus reuteri." Applied and Environmental Microbiology 71, no. 11 (2005): 6769–75. http://dx.doi.org/10.1128/aem.71.11.6769-6775.2005.

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ABSTRACT This study was aimed at evaluating the cloning and expression of three rumen microbial fibrolytic enzyme genes in a strain of Lactobacillus reuteri and investigating the probiotic characteristics of these genetically modified lactobacilli. The Neocallimastix patriciarum xylanase gene xynCDBFV, the Fibrobacter succinogenes β-glucanase (1,3-1,4-β-d-glucan 4-glucanohydrolase [EC 3.2.1.73]) gene, and the Piromyces rhizinflata cellulase gene eglA were cloned in a strain of L. reuteri isolated from the gastrointestinal tract of broilers. The enzymes were expressed and secreted under the con
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49

Ntaikou, I., E. Koutros, and M. Kornaros. "Valorisation of wastepaper using the fibrolytic/hydrogen producing bacterium Ruminococcus albus." Bioresource Technology 100, no. 23 (2009): 5928–33. http://dx.doi.org/10.1016/j.biortech.2009.06.019.

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50

Matulova, M., R. Nouaille, P. Capek, M. Péan, E. Forano, and A. M. Delort. "Degradation of Wheat Straw by Fibrobacter succinogenes S85: a Liquid- and Solid-State Nuclear Magnetic Resonance Study." Applied and Environmental Microbiology 71, no. 3 (2005): 1247–53. http://dx.doi.org/10.1128/aem.71.3.1247-1253.2005.

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ABSTRACT Wheat straw degradation by Fibrobacter succinogenes was monitored by nuclear magnetic resonance (NMR) spectroscopy and chemolytic methods to investigate the activity of an entire fibrolytic system on an intact complex substrate. In situ solid-state NMR with 13C cross-polarization magic angle spinning was used to monitor the modification of the composition and structure of lignocellulosic fibers (of 13C-enriched wheat straw) during the growth of bacteria on this substrate. There was no preferential degradation either of amorphous regions of cellulose versus crystalline regions or of ce
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