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Relevant bibliographies by topics / Fields of Research – 270000 Biological Sciences – 270100 Biochemistry and Cell Biology

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Dissertations / Theses

Academic literature on the topic 'Fields of Research – 270000 Biological Sciences – 270100 Biochemistry and Cell Biology'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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Dissertations / Theses on the topic "Fields of Research – 270000 Biological Sciences – 270100 Biochemistry and Cell Biology"

1

Cooney, Terrence Patrick. "Studies on the biosynthesis of indole-3-acetic acid in tomato shoots." Thesis, University of Auckland, 1989. http://hdl.handle.net/2292/2071.

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The relative contributions of the three main intermediates of indole-3-acetic acid (IAA) biosynthesis from L-tryptophan (L-Trp); indole-3-pyruvate (IPyA), tryptamine (TNH2) and indole-3-acetaldoxime (IAOX), were investigated in vivo in tomato shoots. Initially, L-Trp, D-Trp, IPyA, TNH2 and IAA were purified from shoots, identified by full-scan mass spectrometry and their concentrations measured using gas chromatography with an electron capture detector. High specific activity [5-3H]IAOX and [5-3H]IPyA were synthesized from L-[5-3H]Trp and used as internal standards. Purification of endogenous

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2

Snowden, Kimberley Cathryn. "The molecular response of wheat roots to aluminium stress." Thesis, University of Auckland, 1994. http://hdl.handle.net/2292/1967.

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Aluminium (Al) toxicity to plants is a significant problem, limiting agricultural production in up to 40% of the world's arable soils. In spite of a large amount of research, there is still no consensus on the physiological mechanisms of Al toxicity in plants. In addition, very little is known about the molecular response of plants to Al stress. This body of research was aimed at identifying the changes in gene expression that occurred in the root tips of plants that had been stressed with Al. A cDNA library made from the root tips of Al-treated wheat (Triticum aestivum L., cultivar Warigal) p

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3

Henry, Stephen Michael. "Further insight into the Lewis histo-blood-group system as revealed from study of Polynesian and Caucasian plasma and erythrocyte glycosphingolipids." Thesis, University of Auckland, 1993. http://hdl.handle.net/2292/1975.

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This project involved the study of Lewis and related blood group glycosphingolipid isolated from individuals with normal and aberrant Lewis/secretor phenotypes. The objective was to find a biochemical basis for the unusual expression of Lewis and secretor phenotypes in Polynesians and to use this information to shed light on the "normal" expression of Lewis antigens. By using purified glycolipids, presenting them in the cell free environment of thin layer chromatography to Lewis antibodies and by determining structures by mass spectrometry it has been shown that: l. The Lec epitope is a termin

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4

Hieber, A. David (Andrew David) 1966. "Characterisation of glycoprotein II from bovine adrenal medulla." Thesis, University of Auckland, 1993. http://hdl.handle.net/2292/1988.

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Glycoprotein II (GpII) is a glycoprotein isolated from the membranes of chromaffin granules in the adrenal medulla. The chromaffin granules of the adrenal medulla are responsible for the biosynthesis, storage and secretion of catecholamines, neuropeptides and various proteins. The abundance of chromaffin granules makes them an excellent model to further study the organelles specialized in the synthesis and secretion of hormones and neurotransmitters, from both endocrine and synaptic vesicles. When viewed by two-dimensional electrophoresis GpII is a heterogeneoug glycoprotein (80000-100000 dalt

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5

Hiyama, Jun. "Isolation and characterisation of N-glycans of ovine and human luteinizing hormones." Thesis, University of Auckland, 1991. http://hdl.handle.net/2292/1989.

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Gonadotrophic hormones are heterodimeric glycoproteins and their N-glycans attached to specific amino acid residues are currently thought to play important roles in hormonal biosynthesis, secretion and function. The studies reported in this thesis aimed at isolation and characterisation of structural properties of the N-glycans on ovine and human luteinizing hormones. Initially, chromatographic methods were developed using reverse-phase HPLC for the analytical separation of the three human pituitary glycoprotein hormones and their subunits. Separation of intact oLH and its subunits was also ef

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6

Podivinsky, Ellen. "Molecular studies on actinidin, a cysteine protease from kiwifruit." Thesis, University of Auckland, 1991. http://hdl.handle.net/2292/2001.

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Research in this thesis describes the characterisation of mRNA sequences coding for actinidin, a cysteine protease found in abundance in the fruit of kiwifruit (Actinidia deliciosa). The first step in the characterisation required the isolation of mRNA from ripe kiwifruit tissue. The suitability of a number of RNA extraction procedures was investigated. The method finally adopted differed from that used for unripe fruit tissue, and was chosen as a result of the nature of the polysaccharide that contaminated nucleic acids prepared from extracts of kiwifruit fruit tissue. RNA extracted from ripe

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7

Spiers, Andrew J. (Andrew Julian). "Molecular and genetic analysis of RepA from the P307 RepFIB replicon." Thesis, University of Auckland, 1992. http://hdl.handle.net/2292/2044.

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The work in this Thesis concerns the replication control system of the P307 plasmid RepFIB replicon. The basic replicon occupies ≈ 1.6kb of DNA and contains a single large open reading frame (repA) flanked on either side by a series DNA repeat elements. The organisational structure of fie replicon has placed RepFIB into the Step function class of replicons. The placement of RepFIB within this group, as well as a strong homology between RepFIB and mini-P1, has resulted in a series of predictions concerning the control elements of RepFIB replication. The aim of this work was to test some of thes

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8

Daja, Mirella Maria. "Enzyme activities associated with gonadotropic hormones." Thesis, University of Auckland, 1993. http://hdl.handle.net/2292/2311.

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A structural relationship between gonadotropic hormones and certain types of enzymes has been suggested in previous studies and an investigation into the possibility of enzymatic activity associated with the gonadotropic hormones has been the primary focus of the research presented in this thesis. Partial sequence homology between human chorionic gonadotropin (hCG) and α-chymotrypsin prompted the recent proposal of a tertiary structure of hCG using α-chymotrypsin as a folding template, which suggested the possibility of intrinsic peptidase activity associated with hCG. Highly purified hCG (CR1

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9

Janssen, Bart-Jan. "Agrobacterium-mediated gene transfer into kiwifruit." Thesis, University of Auckland, 1991. http://hdl.handle.net/2292/2313.

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A system has been developed to aid in the establishment of Agrobacterium-mediated transformation for new plant species. A series of binary vectors have been constructed that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from transformed plant cells to be assayed in the presence of Agrobacterium. Preliminary experiments examined the expression of these chimaeric GUS genes in transformed petunia leaf discs. GUS expression was detectable 2 days after inoculation, peaked at 3 – 4 days and then declined; if selection wa

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10

Merriman-Smith, B. Rachelle. "Glucose Transporters in Diabetic Complications of the Lens." Thesis, University of Auckland, 2001. http://hdl.handle.net/2292/2338.

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Lens transparency is primarily maintained by the anaerobic metabolism of glucose. Glucose is transported from the aqueous humuor to the lens epithelial cells however, it has not yet been established how glucose penetrates to the inner part of the lens. The core of the lens is acidic, approximately pH 6.5, as an effect of the accumulation of lactate, the end-product of glycolysis. This confirms that glucose is drawn deep into the core of the lens. Until recently it was assumed that glucose was transported to the core via a gap junction-mediated route by cell-cell diffusion. However, passive dif

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