Academic literature on the topic 'Fish-culture'

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Journal articles on the topic "Fish-culture"

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Battaglene, Stephen C. "Marine Fish Culture." Journal of Experimental Marine Biology and Ecology 254, no. 1 (November 2000): 124–26. http://dx.doi.org/10.1016/s0022-0981(00)00273-2.

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Billard, R. "Endocrinology and fish culture." Fish Physiology and Biochemistry 7, no. 1-6 (June 1989): 49–58. http://dx.doi.org/10.1007/bf00004689.

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Rocha Bispo, Aline Simões da, Jéssica Ferreira Mafra, Paulo Sérgio Pedroso Costa Júnior, Thiago Alves Santos de Oliveira, Elizabeth Amélia Alves Duarte, and Norma Suely Evangelista Barreto. "Formulation of culture media using fish scale bioconversion." SDRP Journal of Earth Sciences & Environmental Studies 4, no. 1 (2018): 534–40. http://dx.doi.org/10.25177/jeses.4.1.ra.433.

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Milstein, A., M. Zoran, and H. J. Krambeck. "Seasonal stratification in fish culture and irrigation reservoirs: potential dangers for fish culture." Aquaculture International 3, no. 2 (June 1995): 116–22. http://dx.doi.org/10.1007/bf00117878.

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Meade, James W. "Allowable Ammonia for Fish Culture." Progressive Fish-Culturist 47, no. 3 (July 1985): 135–45. http://dx.doi.org/10.1577/1548-8640(1985)47<135:aaffc>2.0.co;2.

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dos Santos, Hugo Leandro. "Larva Culture for Fish Species." Environmental Sciences and Ecology: Current Research (ESECR 3, no. 4 (June 7, 2022): 1–2. http://dx.doi.org/10.54026/esecr/1060.

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Sun, Na, and Galina V. Alekseeva. "Fish in Traditional Hezhe Culture." Observatory of Culture 20, no. 6 (December 21, 2023): 574–81. http://dx.doi.org/10.25281/2072-3156-2023-20-6-574-581.

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Representatives of the Hezhe people (Nanai people) live in Russia and China. This article is about fish in their traditional culture. Fish and everything related to it can be considered a cultural symbol of the Hezhe people. This is due to the peculiarities of the culture of the region located along the Amur, Sungari and Ussuri rivers. Analyses of historical documents and artistic works show the great importance of working with fish skin in the life of the Hezhe. Material production and spiritual life of the Hezhe have been closely connected with fish for many generations. It has been revealed that the high fertility of fish, one of the main sources of food and material for sewing clothes, caused the Hezhe people to revere fish in ancient times. It has been established that the Hezhe people had a rich culture. Fish became one of the most widespread zoomorphic motifs of Hezhe ornamental art. The image of fish on the products was given a high cultural meaning. This image left a vivid trace in both Chinese and Russian culture — from totemism to decorative items. The article presents an analysis of fish as a cultural symbol based on the materials of Chinese and Russian museum collections. The study of fish in the traditional culture of Hezhe will expand the existing knowledge about the nature and specificity of ethno-cultural interaction between China and the peoples of the Amur region for many centuries. An attempt is made to tell about the specifics of Chinese cultural meaning of decorative items based on fish skin. For the first time the authors treat the ornamentation of fish patterns in the traditional culture of Hezhe in a systematized form. This will allow researchers to familiarize themselves with information from Chinese sources to identify typical images of fish in Hezhe for further study of the topic of fish skin processing as art in Russia.
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De Groot, S. J. "Textbook of fish culture — Breeding and cultivation of fish." Aquaculture 65, no. 1 (August 1987): 91. http://dx.doi.org/10.1016/0044-8486(87)90274-2.

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Halim, Abdullah. "Cage Fish Culture as Sustainable Fish Farms on Salma Dam." Asian Journal of Research in Animal and Veterinary Sciences 3, no. 4 (September 10, 2020): 292–99. http://dx.doi.org/10.9734/ajravs/2020/v3i4134.

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Suguna, T. "Management of Stress in Culture Fish." International Journal of Bio-resource and Stress Management 11, no. 6 (December 31, 2020): 607–12. http://dx.doi.org/10.23910/1.2020.2152a.

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Over the last three decades, the commercial aquaculture has experienced spectacular growth. Many species have gone from small scale regional production to large scale global production. Concomitant with the rapid growth there also has been the increased occurrence of problems that accompany all agricultural endeavours. All the problems are stress influenced leading to diseases, impacting the profitability of the industries. In aquaculture also inspite of the unprecedented development of the intensified culture practices many economical problems have arise that are threatening the sustainability of culture systems. The root cause for all is stress. The word, “stress” is very common butreflects vast effective results. It is an invisible factor, influencing the survivality, growth, reproduction, production in culture fish especially. It is much easier for diseases to proliferate in the culture environment than in wild. Defining what levels of stressors are normal and acceptable is not easy. A level of stressor that is problematic under one set of environmental conditions might not be the same under another. The susceptibility of disease occurrence differs within species and age groups. Different stress factor such as inadequate physico chemical and microbial quality of culture water, poor nutritional stems and high stocking density can cause infection by opportunistic pathogens. In aquaculture, the stress plays major role on production, productivity, sustainability of the culture, economic loss and degradation of economic standards. A summation of causes for the acute and chronic stressors will enlighten the aqua farmers, scientists and fishery officials in designing environmentally friendly controlling measures, in obtaining higher yields.
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Dissertations / Theses on the topic "Fish-culture"

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Wong, Yuen-yee Queenie. "Mariculture practices in relation to water quality and the nearshore marine environment in Hong Kong /." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22264188.

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Wu, Mei-yee. "Study on the feasibility of setting up a fish fry hatchery in Hong Kong." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43784501.

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Varvarigos, Panagiotis. "Microcomputer based fish farm production planning : the development of a microcomputerised data recording and production decision support system for individual fish farmers and its implementation on a fresh water trout hatchery." Thesis, University of Stirling, 1987. http://hdl.handle.net/1893/24822.

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In order to develop a system that could provide fish farmers with information to monitor stock performance and plan for a timely production, a computerised recording system was designed and tested on a trout hatchery in Scotland. It facilitated routine data capture on site with a small hand-held computer, programmed in BASIC language, which subsequently downloaded the data to a central desk-top microcomputer for further processing. Both direct and long-distance transmission via the telephone network using modems were possible. The LOTUS 1-2-3 general purpose software package, running on the microcomputer, was customised using 'macro' commands to accept the transmitted data and create files for storage on 'floppy' or 'hard' magnetic disks. Further information could be calculated and graphs and summary reports for stock control could be generated at will. Other customised LOTUS worksheets were developed to allow identification and access of specific historical data in order to calibrate regression equations and provide growth predictions for particular fish types. This information combined with cost and pricing details was further utilised by a linear programming package. Guidelines on optimum policies were formulated and sensitivity analyses could be performed. This production information system was implemented on the IBM-PC and the OLIVETTI M24 desk-top microcomputers and as field devices the SHARP PC 1500A and the HUSKY HUNTER were used. Since existing technology and 'off-the-shelf' software were utilised, the developed information system can be easily adjusted to suit the individual needs of different fish farms. However, the most important requirement for successful implementation would be the commitment and enthusiasm of the fish farm manager.
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Van, der Merwe Johan Philip. "Vergelyking van Tilapia (Oreochromis Mossambicus)en Koi (Ciprinus Carpio) in verskillende produksiestelsels." Thesis, Port Elizabeth Technikon, 2002. http://hdl.handle.net/10948/114.

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Die studie was uitgevoer om te bepaal of warmwaterspesies soos tilapia (Oreochromis mossambicus) en koi (Ciprinus carpio) gebruik kan word as deel van 'n roterende akwakultuur produksie sisteem in die Wes-Kaap. Drie produksiestelsels (drywende hokstelsel; gronddamme en hersirkuleringseenheid) was ge-evalueer vir beide tilapia en koi produksie. Twee verskillende diëte (tilapia, 32% proteien, 'n lae waarde dieet en koi, 32% proteien, 'n hoë waarde dieet) was ook ge-evalueer vir die effek op koi produksie. Tilapia- en koi vingerlinge was teen dieselfde lading (250/hok) in elke produksiestelsel geplaas. By die begin van die proewe het die tilapia vingerlinge 'n gemiddelde gewig van 60-80g en die koi vingerlinge 'n gemiddelde lengte van 30-40 mm gehad. Die visse is twee keer per dag gevoer en data is op 'n maandelikse basis oor 'n periode van 84 dae versamel. Produksie data vir die tilapia versamel was: 1) die gemiddelde daaglikse toename, 2) die totale voer verbruik, 3) water temperature en 4) mortaliteite is daagliks aangeteken. Die produksie data versamel vir koi wat twee verskillende diëte ontvang het in drie verskillende produksiestelsels was: 1) die gemiddelde daaglikse toename in lengte, 2) die totale voer verbruik, 3) water temperature en 4) mortaliteite is daagliks aangeteken. Die resultate van die tilapia studie het aangedui dat tilapia in die hersirkuleeringseenheid het deurgaans 'n swaarder gewig gehad (p<0.05) as die van die drywende hokstelsel en gronddam stelsel. Terwyl die gronddam stelsel weer 'n swaarder gewig gehad het as die drywende hokstelsel behalwe vir April (171,67 / 39,49 vs 159,33 / 22,15). Nieteenstaande die verskil in begin gewig en eind gewig in die guns van die tilapia in die hersirkuleringseenheid teenoor die ander twee stelsels het die GDT tussen die drywende hokstelsel en die hersirkuleringseenheid nie noemenswaardig verskil nie (1,20 / 0,15 vs 1,40 / 0,24). Die tilapia in die gronddamme het egter swakker (p<0,05) gewigstoename van 0,72 / 0,32 g gehad teenoor die tilapia in die drywende hokstesel 1,40 / 0,24 g en 1,20 / 0,15 g van die tilapia in die hersirkuleringseenheid. Tilapia in die gronddamme het dus ongeveer 45% swakker gemiddelde gewigstoename getoon as die van die drywende hokstelsel en hersirkuleringseenheid. Die produksiestelsel het 'n hoogs betekenisvolle invloed (p<0,001) op die groeipotensiaal van tilapia gehad in hierdie studie. Die gemiddelde totale hoeveelheid voer verbruik was nie statisties verskillend tussen die drywende hokstelsel en gronddam stelsel nie (29,83 / 2,66 vs 30,83 / 5,42), maar wel (p<0,05) vir die hersirkuleringseenheid (29,94 / 0,88). Die VOV het nie verskil tussen die drywende hokstelsel en hersirkuleringeenheid, maar daar was wel 'n verskil (p<0,05) tussen hierdie twee stelsels en die van die gronddamme. Die totale gemiddelde mortaliteite van tilapia was die hoogste (p<0,05) vir die drywende hokstelsel in vergelyking met die gronddamme en hersirkuleringseenheid. Die drywende hokstelsel het 14,67 / 14,61% meer mortaliteite gehad as die gronddamme en hersirkuleringeenheid. Die koi studie se resultate dui aan dat die tipe stelsel het 'n hoogs betekenisvolle (p<0,001) invloed gehad op die gemiddelde toename in lengte van die koi vis vir die somerseisoen. Die tipe dieet het geen invloed op die toename in lengte van koi vis vir al drie die stelsels ge-evalueer, maar die interaksie tussen dieet en die tipe stelsel was wel betekenisvol (p<0.05). Die interaksie tussen die tipe dieet en die tipe stelsel was die hoogste vir die groddamme 136,41 / 32,46 mm en 136,25 / 33,08 mm vir beide tilapia en die koi diëte gevoer. Die gemiddelde totale voer wat deur koi visse in die drie stelsels verbruik is, het wel statisties van mekaar verskil (p<0,05). Koi visse in die gronddam stelsel het meer voer verbruik vir beide die tilapia- en koi dieet (31,03 / 2,01 en 29,67 / 3,57) in vergelyking met die (26,35 / 2,44 ; 26,98 / 0,49 en 5,50 # 0,52 en 6,13 / 0,22) vir die drywende hokke en hersirkuleringseenheid stelsels onderskiedelik. Die % mortaliteite was betekenisvol (p<0,05) vir die verskillende stelsels. Koi visse in die hersirkuleringseenheid het die hoogste mortaliteite gehad vir beide die tilapia en die koi diëte (65,50 / 18,07% en 79,50 / 38,27%) terwyl koi visse in die gronddamme die laagste mortaliteite ondervind het (1,20 / 1,20% en 2,33 / 4,89%) vir beide die tilapia en koi diëte. Die koi in die gronddam stelsel het die beste resultate gelewer en dit is ook moontlik om koi 'n lae waarde dieet te voer sonder nadelige effekte op produksie.
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Lam, Kwok-hung Ken. "Sustainable development and property rights : a case study of pond fish culture in Hong Kong /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B20353170.

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Roberts, Laura Jayne. "Improving the survival and fitness of hatchery-reared salmonids in restoration programmes." Thesis, Swansea University, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678416.

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Bolduc, Melanie B. "Long-term effects of habitat and management changes on steelhead production results from an individual-based model." Link to electronic thesis, 2006. http://www.wpi.edu/Pubs/ETD/Available/etd-050406-112805/.

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Wang, Yin-Han. "Model and software development for predicting fish growth in trout raceways." Morgantown, W. Va. : [West Virginia University Libraries], 2006. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4751.

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Thesis (M.S.)--West Virginia University, 2006.
Title from document title page. Document formatted into pages; contains xii, 105 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 47).
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Triminio, de Meyer Suyapa. "Tilapia fingerling production in Honduras." Auburn, Ala., 2005. http://repo.lib.auburn.edu/2005%20Summer/master's/MEYER_SUYAPA_0.pdf.

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Chu, Yat-chun Jackson. "Education units of marine fish farming." Hong Kong : University of Hong Kong, 1995. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2594650x.

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Books on the topic "Fish-culture"

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Tucker, John W. Marine Fish Culture. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6.

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Tucker, John W. Marine fish culture. Boston: Kluwer Academic Pub., 1998.

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Lakra, W. S. Fish cell & tissue culture. Lucknow: National Bureau of Fish Genetic Resources, 2012.

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Soderberg, Richard W. Flowing water fish culture. Boca Raton: Lewis Publishers, 1995.

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Anibeze, Chike I. Fish farming in Nigeria. Enugu, Nigeria: Delta of Nigeria, 1995.

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Pouomogne, Victor. Pisciculture en milieu tropical africain: Comment produire du poisson à cout modéré : (des exemples du Cameroun). Yaoundé: Presses universitaires d'Afrique, 1998.

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Motohiro, Ohashi, Malawi National Aquaculture Center, Malawi. Fisheries Dept., Kokusai kyōryoku Jigyōdan, and Research Project for Small-Scale Aquaculture of Malawian Indigenous Species., eds. Technical report and instruction of aquaculture in Malawi on rearing technique development. [Domasi, Malawi]: National Aquaculture Center Domasi, 1999.

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S, Duvarova A., and Skli͡a︡rov V. I͡A︡, eds. Intensivnoe ryborazvedenie v Krasnodarskom krae. Sankt-Peterburg: GosNIORKh, 1993.

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Angell, Charles L. Silvipisciculture project in Sunderbans, West Bengal: A summary report of BOBP's assistance. Madras: Bay of Bengal Programme for Fisheries Development, 1990.

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Moskul, G. A. Rybokhozi͡a︡ĭstvennoe osvoenie Krasnodarskogo vodokhranilishcha. Sankt-Peterburg: KrasNIIRKh, 1994.

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Book chapters on the topic "Fish-culture"

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Tucker, John W. "Culture Units." In Marine Fish Culture, 175–210. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_6.

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Tucker, John W. "Introduction." In Marine Fish Culture, 1–16. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_1.

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Tucker, John W. "Energetics." In Marine Fish Culture, 469–79. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_10.

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Tucker, John W. "Maintaining Healthy Marine Fish." In Marine Fish Culture, 481–520. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_11.

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Tucker, John W. "Handling and Transporting Marine Fish." In Marine Fish Culture, 521–32. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_12.

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Tucker, John W. "Culture of Established and Potential Species—Food Fish." In Marine Fish Culture, 533–74. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_13.

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Tucker, John W. "Culture of Established and Potential Species—Bait Fish." In Marine Fish Culture, 575–76. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_14.

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Tucker, John W. "Culture of Established and Potential Species—Ornamental Fish." In Marine Fish Culture, 577–86. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_15.

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Tucker, John W. "The Future of Marine Fish Culture." In Marine Fish Culture, 587–88. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_16.

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Tucker, John W. "Characteristics of Marine Fish." In Marine Fish Culture, 17–48. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-4911-6_2.

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Conference papers on the topic "Fish-culture"

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Lopez-Tejeida, Samuel, Leticia Felix-Cuencas, Jesus Josafat De Leon-Ramirez, Laura Berenice Flores-Tejeida, Maribel Villegas-Villegas, and Juan Fernando Garcia-Trejo. "Methods to measure nitrogen content in fish culture: A review." In 2021 XVII International Engineering Congress (CONIIN). IEEE, 2021. http://dx.doi.org/10.1109/coniin54356.2021.9634790.

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Sung, Wen-Tsai, Jui-Ho Chen, and Sung-Jung Hsiao. "Fish pond culture via fuzzy and self-adaptive data fusion application." In 2017 IEEE International Conference on Systems, Man and Cybernetics (SMC). IEEE, 2017. http://dx.doi.org/10.1109/smc.2017.8123082.

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Tribowo, R. Ismu, and Fithria Novianti. "Drainage design on peat land and its utilization for fish culture." In PROCEEDINGS OF THE 3RD INTERNATIONAL SYMPOSIUM ON APPLIED CHEMISTRY 2017. Author(s), 2017. http://dx.doi.org/10.1063/1.5011860.

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Li, Daoliang, and Xiuna Zhu. "CDMA-Based Remote Wireless Water Quality Monitoring System for Intensive Fish Culture." In 2009 WRI International Conference on Communications and Mobile Computing (CMC). IEEE, 2009. http://dx.doi.org/10.1109/cmc.2009.241.

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Nedyalkov, Ivaylo, Todd Guerdat, Drue Seksinsky, Sylvia Romero, Justin Stickney, and Ethan Pirie. "Numerical and Experimental Investigation of Flow in Fish Tanks for Small-Scale Aquaponic Systems." In ASME 2017 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/fedsm2017-69395.

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Aquaponic systems combine recirculating aquaculture (growing of fish) with hydroponics (growing of plants in water). The fish in the recirculating aquaculture systems provide nutrients for the plants and the plants remove excess nutrients from the water, making these systems more efficient than traditional farming methods in terms of nutrient utilization. Small, recirculating aquaponic systems may provide a more sustainable and cost-effective alternative for securing food supply in both developing and developed nations. Recirculating aquaculture systems tend to be capital-intensive and require significant power to circulate the water in the fish tanks, which helps with the removal of waste and the distribution of oxygen. To reduce capital costs, alternative, culture vessels made from locally available materials were investigated (i.e. square-shaped tanks, and international bulk containers - IBC). These non-standard shaped culture tanks, pose an additional challenge for proper circulation of the water as compared to traditional round tanks. To address the issue of circulation, numerical and experimental data were obtained for rectangular containers. The numerical results were obtained using OpenFoam models of the experimental setup. The experimental data were obtained by measuring flow velocities in an IBC tank using Acoustic Doppler Velocimetry. Currently the experimental data show good repeatability when data are taken for at least five minutes at each position in the tank. The focus of the continuing work is to establish a good agreement between numerical and experimental results. Ultimately the study will contribute to the design of cost-effective recirculating aquaponic fish and plant systems which require lower capital expenditures and achieve energy-efficient circulation of water in the fish culture tanks.
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Roomiani, Laleh, Sara Nikbakht, and Aboalfazl Askary Sary. "Heavy Metal Bioaccumulation by the Aquatic Macrophyte, Phragmites Australis in Fish Culture Ponds." In 6th Annual International Conference on Sustainable Energy and Environmental Sciences (SEES 2017). Global Science & Technology Forum (GSTF), 2017. http://dx.doi.org/10.5176/2251-189x_sees17.10.

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Jino Ramson, S. R., Deepthi Bhavanam, Srirupa Draksharam, anjeet Kumar, D. Jackuline Moni, and A. Alfred Kirubaraj. "Sensor Networks based Water Quality Monitoring Systems for Intensive Fish Culture -A Review." In 2018 4th International Conference on Devices, Circuits and Systems (ICDCS). IEEE, 2018. http://dx.doi.org/10.1109/icdcsyst.2018.8605146.

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Hansen, Irina-Emily, Ola Jon Mork, and Paul Steffen Kleppe. "Design, testing and operation of fish processing lines - can simulation tools and artificial intelligence be a gamechanger?" In 38th ECMS International Conference on Modelling and Simulation. ECMS, 2024. http://dx.doi.org/10.7148/2024-0013.

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The oceans play an important role in the world's food supply, so it is very important that we can develop sustainable, safe, and cost-effective production and processing of fish that also safeguards fish welfare. The aim of this research work is to explore if state of the art simulation tools and artificial intelligence, can improve the design, manufacturing, commissioning, and operation of fish processing. The research work takes a broad perspective and describes overall challenges related to fish processing, where the product – the fish – has numerous species, sizes, shapes, and seasonal variations. Processing of fish can take place on trawlers operating on the sea and on land for example processing of aqua culture salmon from. The owners of fish processing lines, have their unique approach and experience, and every fish processing line will be a one-of-a-kind design. Design, testing and operation of fish processing lines can therefore be time consuming, expensive with use of dead fishes either in a physical set up in a workshop or in worst case happen under fish operation in the Barents Sea. If it is possible to set up a simulation scenario with a set of random fish types and random batches of fish and fish processing line operation in rolling sea environment, this can reveal weaknesses with the fish processing line already in the design stage. The research team has set up three early-stage trials of simulation; first the flow of fish on a transport roller, secondly design and test of a singulation machine – machine organizing fishes in a queue for processing –in simulation environment, thirdly shows how simulation can contribute to a more precise and intelligent processing of the fish. The research work indicates that simulation is a promising tool for solving challenges within fish processing. The research work also suggests the possible benefits of simulation for the fish processing industry.
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SHITSUKANE, Aggrey, Mathew EGESSA, Paul ORINA, Kelvin OMIENO, and Mathews AMUTI. "Integrating Fuzzy Logic, Solar Power and IoT for Real-Time Water Quality Monitoring in Cage Fish Culture." In 2024 IST-Africa Conference (IST-Africa). IEEE, 2024. http://dx.doi.org/10.23919/ist-africa63983.2024.10569527.

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Le, Ngoc-Tran, Claude Vo, and Ngoc Dang Khoa Tran. "Design and implement a monitoring and early warning system of water quality for cage fish culture on Chava river." In THE 1ST INTERNATIONAL CONFERENCE ON INNOVATIONS FOR COMPUTING, ENGINEERING AND MATERIALS, 2021: ICEM, 2021. AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0068352.

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Reports on the topic "Fish-culture"

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Colt, John, Kris Orwicz, and Gerald R. Bouck. A Survey and Resource Materials on the Use of Oxygen Supplementation in Fish Culture. Office of Scientific and Technical Information (OSTI), September 1988. http://dx.doi.org/10.2172/920031.

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Harry, Raymond. Effects of Types 1 and 2 Herpes Simplex Viruses on Several Fish Tissue Culture Systems. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.2602.

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Mitchell, Brian G., Amir Neori, Charles Yarish, D. Allen Davis, Tzachi Samocha, and Lior Guttman. The use of aquaculture effluents in spray culture for the production of high protein macroalgae for shrimp aqua-feeds. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7597934.bard.

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The FAO has projected a doubling in world demand for seafood during the 21 ed from aquaculture of marine fish and shrimps fed primarily on fishmeal-based aquafeeds. However, current practices of high intensity monoculture of shrimp in coastal ponds and fish in offshore pens have been strongly criticized as being ecologically and socially unsustainable. This view derives from un- checked eutrophication of coastal marine ecosystems from fish farm effluents, and the destruction of coastal estuarine ecosystems by shrimp farm constructions, plus aquaculture’s reliance on wild-caught small fish - which are excellent food for humans, but instead are rendered into fishmeal and fish oil for formulating aquafeeds. Fishmeal-sparing and waste- reduction aquafeeds can only delay the time when fed aquaculture product are priced out of affordability for most consumers. Additionally, replacement of fishmeal protein and fish oil by terrestrial plant sources such as soybean meal and oil directly raises food costs for human communities in developing nations. New formulations incorporating sustainably-produced marine algal proteins and oils are growing in acceptance as viable and practical alternatives. This BARD collaborative research project investigated a sustainable water-sparing spray/drip culture method for producing high-protein marine macrophyte meals for incorporation into marine shrimp and fish diets. The spray culture work was conducted at laboratory-scale in the USA (UCSD-SIO) using selected Gracilariaand Ulvastrains isolated and supplied by UCONN, and outdoors at pilot-scale in Israel (IOLR-NCM) using local strains of Ulvasp., and nitrogen/phosphorus-enriched fish farm effluent to fertilize the spray cultures and produce seaweed biomass and meals containing up to 27% raw protein (dry weight content). Auburn University (USA) in consultation with TAMUS (USA) used the IOLR meals to formulate diets and conduct marine shrimp feeding trials, which resulted in mixed outcomes, indicating further work was needed to chemically identify and remove anti-nutritional elements present in the IOLR-produced seaweed meals.
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BONJOUR, Lucas, Myriam STERNBERG, and Élisabeth VEYRAT. Study of Cod reserves from the La Hougue Battle Shipwrecks (1692) through ichthyofauna remains: Supply and food aboard. Honor Frost Foundation, 2023. http://dx.doi.org/10.33583/mags2021.02.

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In 1692, in the context of the Nine Years War (1689-1697), Admiral Tourville’s French fleet faced the Anglo-Dutch alliance along the Norman coast. Five large French men-of-war were reduced to ashes close to Tatihou island in St-Vaast-La Hougue bay (Manche county, France). Discovered in 1985, these shipwrecks have been excavated from 1990 to 1995 by Michel L’Hour and Élisabeth Veyrat, from the Département des Recherches Archéologiques Subaquatiques et Sous-Marines (DRASSM, Ministry of Culture). Among the numerous remains, fish bones were found in large quantities on one of the wrecks. Preliminary studies conducted between 1990 and 1992 by Myriam Sternberg determined that the fish remains were cod (Gadus morhua L.) which were reserved for food aboard. The data recovery during the year 2020-2021 allowed the acquisition of new knowledge on the supplying and the process carried out on this fish.
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Bacharach, Eran, W. Ian Lipkin, and Avigdor Eldar. Identification of the etiological agent of tilapia disease in the Lake of Galillee. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7597932.bard.

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Background to the topic. Tilapines serve as the second most important group of farmed fish worldwide. Massive mortality of wild and cultured tilapia has been observed recently in Israel but the pathogen of this disease has not been identified. We proposed to identify the agent responsible for disease.  Major conclusions, solutions, achievements. We characterized the lesions in diseased fish and found that the brain was one of the affected organs. We found conditions to isolate from brains of diseased fish the etiological agent of the tilapia disease and to propagate it in cell culture. This led to the identification of the pathogen as a novel RNA virus, which we named Tilapia Lake Virus (TiLV). Electron microscopy of TiLV revealed virion-like particles and ether/chloroform-sensitivity assays demonstrated that TiLV is enveloped. Low passage TiLV, injected intra-peritoneally to tilapia, induced a disease with over 80% mortality. Cohabitation of healthy with diseased fish demonstrated that the disease is contagious, and that mortalities occur within few days. Fish surviving initial mortality were immune to further TiLV infections, suggesting the mounting of protective immune response. Screening cDNA libraries and high throughput sequencing determined the sequence of TiLV genome. This demonstrated that TiLV is indeed a novel virus and allowed the design of a PCRbased diagnostic test.  Implications, both scientific and agricultural. The characterization of a novel, emerging RNA virus that imposes major threat to the tilapia industry, enables the specific identification of the virus in tilapines. This allows prompt screening and surveillance of TiLV, epidemiological studies, and disease containment. This also potentially opens the way for the development of vaccines against TiLV.
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Kotler, Moshe, Larry Hanson, and Shane Burgess. Replication Defective Cyprinid Herpes Virus-3 (CyHV-3) as a Combined Prophylactic Vaccine in Carps. United States Department of Agriculture, December 2010. http://dx.doi.org/10.32747/2010.7697104.bard.

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Aquacultured koi and common carp fish (Cyprinus carpio) are intensively bred as ornamental and food fish in many countries worldwide. Hatcheries of carp and koi have recently suffered massive financial damages due to two viral diseases caused by the Cyprinid herpesvirus-3 (CyHV-3), previously designated as Carp Interstitial Nephritis and Gill Necrosis Virus (CNGV) and Koi herpesvirus (KHV), and by the Spring Viremia of Carp Virus (SVCV). CyHV-3 is a large dsDNA virus, which is infectious mostly to koi and common carp, while SVCV is a rhabdovirus with a relatively broad host range. Both viruses induce contagious disease with mortality rate up to 90%. Strategies for the control of viral infection in fish are of limited use. While efforts to prevent introduction of infectious agents into culture facilities are desirable, such exclusion strategies are far from fail-safe. Extensive vaccination methods that are useful for use in aquaculture facilities produce weak immunity, when used with proteins or inactivated viruses. Methods to overcome this obstacle are to vaccinate the fish with large amounts of antigen and/or use adjuvant and immune modulators over a long period. These techniques usually require individual handling of the fish. On the other hand, live attenuated virus is efficient and economical when used as an immersionvaccine. However, this technique poses certain environmental risks and thus may be difficult to license and scale up. Another option is a vaccine based on the replication defective virus (RDV) (pseudovirus), which can infect cells, but is unable to produce infectious particles. This vaccine may circumvent many of the problems related to attenuated-live vaccine (e.g., inadvertent infection and reversion to the virulent strain).
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Saillant, Eric, Jason Lemus, and James Franks. Culture of Lobotes surinamensis (Tripletail). Mississippi Department of Marine Resources, January 2014. http://dx.doi.org/10.18785/ose.001.

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The Tripletail, Lobotes surinamensis, is a pelagic fish found in tropical and sub-tropical waters of all oceans. Tripletails are often associated with floating debris and make frequent incursions in bays and estuaries where they are targeted by recreational fishermen. In Mississippi waters the species is typically present during the late spring and summer season that also correspond to the period of sexual maturation and spawning (Brown-Peterson and Franks 2001). Tripletail is appreciated as a gamefish but is also prized for its flesh of superior quality. The fast growth rate of juveniles in captivity documented by Franks et al. (2001) and the excellent quality of Tripletail flesh both contribute to the potential of this species for marine aquaculture. In addition, the production of cultured juveniles would be precious to develop a better understanding of the biology, early life history and habitat use of Tripletail larvae and juveniles, a topic largely undocumented to date, through experimental releases and controlled studies. The culture of tripletail thus supports the Tidelands Trust Fund Program through improved conservation of natural resources, potential enhancement of fisheries productivity and potential development of a new economic activity on the Gulf coast producing tripletail via aquaculture. The Objective of this project was to initiate development of methods and techniques needed to spawn captive held tripletail broodfish and raise their offspring to evaluate their growth and development in captivity. In this report we will present the results of studies aiming to develop methods and protocols for captive spawning of tripletail and the first data obtained on the early development of tripletail larvae. A major issue that was encountered with tripletail broodstock development during the project lied in the difficulties associated with identifying the sex of adults caught in the wild and candidates for being incorporated in mating sets for spawning. This issue was addressed during the course of the project by examining the potential of a non-lethal method of hormonal sexing. The results of these preliminary investigations are presented in the third part of this report. All protocols used in the project were determined with the guidance of the Institutional Animal Care and Use Committee (IACUC) of the University of Southern Mississippi (USM IACUC protocol number 10100108).
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Bercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7695593.bard.

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Original objectives and revisions-The proposed research included these original objectives: field validation of diagnostic tests (PCR), the development and evaluation of new sensitive tools (LC-PCR/TaqManPCR, antibody detection by ELISA) including their use to study the ecology and the epidemiology of KHV (virus distribution in the environment and native cyprinids) and the carrier status of fish exposed experimentally or naturally to KHV (sites of virus replication and potential persistence or latency). In the course of the study we completed the genome sequence of KHV and developed a DNA array to study the expression of KHV genes in different conditions. Background to the topics-Mass mortality of koi or common carp has been observed in Israel, USA, Europe and Asia. These outbreaks have reduced exports of koi from Israel and have created fear about production, import, and movements of koi and have raised concerns about potential impacts on native cyprinid populations in the U.S.A. Major conclusions-A suite of new diagnostic tools was developed that included 3 PCR assays for detection of KHV DNA in cell culture and fish tissues and an ELISA assay capable of detecting anti-KHV antibodies in the serum of koi and common carp. The TKPCR assay developed during the grant has become an internationally accepted gold standard for detection of viral DNA. Additionally, the ELISA developed for detecting serum anti-KHV antibodies is now in wide use as a major nonlethal screening tool for evaluating virus status of koi and common carp populations. Real time PCR assays have been able to detect viral DNA in the internal organs of survivors of natural and wild type vaccine exposures at 1 and 10³ genome equivalents at 7 months after exposure. In addition, vaccinated fish were able to transmit the virus to naive fish. Potential control utilizing hybrids of goldfish and common carp for production demonstrated they were considerably more resistant than pure common carp or koi to both KHV (CyHV-3). There was no evidence that goldfish or other tested endemic cyprinids species were susceptible to KHV. The complete genomic sequencing of 3 strains from Japan, the USA, and Israel revealed a 295 kbp genome containing a 22 kbp terminal direct repeat encoding clear gene homologs to other fish herpesviruses in the family Herpesviridae. The genome encodes156 unique protein-coding genes, eight of which are duplicated in the terminal repeat. Four to seven genes are fragmented and the loss of these genes may be associated with the high virulence of the virus. Viral gene expression was studies by a newly developed chip which has allowed verification of transcription of most all hypothetical genes (ORFs) as well as their kinetics. Implications, both scientific and agricultural- The results from this study have immediate application for the control and management of KHV. The proposal provides elements key to disease management with improved diagnostic tools. Studies on the ecology of the virus also provide insights into management of the virus at the farms that farmers will be able to apply immediately to reduce risks of infections. Lastly, critical issues that surround present procedures used to create “resistant fish” must be be resolved (e.g. carriers, risks, etc.). Currently stamping out may be effective in eradicating the disease. The emerging disease caused by KHV continues to spread. With the economic importance of koi and carp and the vast international movements of koi for the hobby, this disease has the potential for even further spread. The results from our studies form a critical component of a comprehensive program to curtail this emerging pathogen at the local, regional and international levels.
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Funkenstein, Bruria, and Cunming Duan. GH-IGF Axis in Sparus aurata: Possible Applications to Genetic Selection. United States Department of Agriculture, November 2000. http://dx.doi.org/10.32747/2000.7580665.bard.

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Many factors affect growth rate in fish: environmental, nutritional, genetics and endogenous (physiological) factors. Endogenous control of growth is very complex and many hormone systems are involved. Nevertheless, it is well accepted that growth hormone (GH) plays a major role in stimulating somatic growth. Although it is now clear that most, if not all, components of the GH-IGF axis exist in fish, we are still far from understanding how fish grow. In our project we used as the experimental system a marine fish, the gilthead sea bream (Sparus aurata), which inhabits lagoons along the Mediterranean and Atlantic coasts of Europe, and represents one of the most important fish species used in the mariculture industry in the Mediterranean region, including Israel. Production of Sparus is rapidly growing, however, in order for this production to stay competitive, the farming of this fish species has to intensify and become more efficient. One drawback, still, in Sparus extensive culture is that it grows relatively slow. In addition, it is now clear that growth and reproduction are physiological interrelated processes that affect each other. In particular sexual maturation (puberty) is known to be closely related to growth rate in fish as it is in mammals, indicating interactions between the somatotropic and gonadotropic axes. The goal of our project was to try to identify the rate-limiting components(s) in Sparus aurata GH-IGF system which might explain its slow growth by studying the ontogeny of growth-related genes: GH, GH receptor, IGF-I, IGF-II, IGF receptor, IGF-binding proteins (IGFBPs) and Pit-1 during early stages of development of Sparus aurata larvae from slow and fast growing lines. Our project was a continuation of a previous BARD project and could be divided into five major parts: i) obtaining additional tools to those obtained in the previous project that are necessary to carry out the developmental study; ii) the developmental expression of growth-related genes and their cellular localization; iii) tissue-specific expression and effect of GH on expression of growth-related genes; iv) possible relationship between GH gene structure, growth rate and genetic selection; v) the possible role of the IGF system in gonadal development. The major findings of our research can be summarized as follows: 1) The cDNAs (complete or partial) coding for Sparus IGFBP-2, GH receptor and Pit-1 were cloned. Sequence comparison reveals that the primary structure of IGFBP-2 protein is 43-49% identical to that of zebrafish and other vertebrates. Intensive efforts resulted in cloning a fragment of 138 nucleotides, coding for 46 amino acids in the proximal end of the intracellular domain of GH receptor. This is the first fish GH receptor cDNA that had been cloned to date. The cloned fragment will enable us to complete the GH - receptor cloning. 2) IGF-I, IGF-II, IGFBP-2, and IGF receptor transcripts were detected by RT-PCR method throughout development in unfertilized eggs, embryos, and larvae suggesting that these mRNAs are products of both the maternal and the embryonic genomes. Preliminary RT-PCR analysis suggest that GH receptor transcript is present in post-hatching larvae already on day 1. 3) IGF-1R transcripts were detected in all tissues tested by RT-PCR with highest levels in gill cartilage, skin, kidney, heart, pyloric caeca, and brain. Northern blot analysis detected IGF receptor only in gonads, brain and gill cartilage but not in muscle; GH increased slightly brain and gill cartilage IGF-1R mRNA levels. 4) IGFBP-2 transcript were detected only in liver and gonads, when analyzed by Northern blots; RT-PCR analysis revealed expression in all tissues studied, with the highest levels found in liver, skin, gonad and pyloric caeca. 5) Expression of IGF-I, IGF-II, IGF-1R and IGFBP-2 was analyzed during gonadal development. High levels of IGF-I and IGFBP-2 expression were found in bisexual young gonads, which decreased during gonadal development. Regardless of maturational stage, IGF-II levels were higher than those of IGF-L 6) The GH gene was cloned and its structure was characterized. It contains minisatellites of tandem repeats in the first and third introns that result in high level of genetic polymorphism. 7) Analysis of the presence of IGF-I and two types of IGF receptor by immunohistochemistry revealed tissue- and stage-specific expression during larval development. Immunohistochemistry also showed that IGF-I and its receptors are present in both testicular and ovarian cells. Although at this stage we are not able to pinpoint which is the rate-limiting step causing the slow growth of Sparus aurata, our project (together with the previous BARD) yielded a great number of experimental tools both DNA probes and antibodies that will enable further studies on the factors regulating growth in Sparus aurata. Our expression studies and cellular localization shed new light on the tissue and developmental expression of growth-related genes in fish.
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Yaron, Zvi, Martin P. Schreibman, Abigail Elizur, and Yonathan Zohar. Advancing Puberty in the Black Carp (Mylopharyngodon Piceus) and the Striped Bass (Morone Saxatilis). United States Department of Agriculture, August 1993. http://dx.doi.org/10.32747/1993.7568102.bard.

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The black carp (bc)GtH IIb cDNA was amplified and isolated, cloned and sequenced. Comparison of the bcGtH IIb deduced a.a. sequence with that of GtH IIb from other teleosts revealed high homology to cyprinid species and a lower homology to salmonid or perciform fish. The gene coding for the GtH IIb was isolated and sequenced. Three bc recombinant phages which hybridized to the goldfish GtH Ib cDNA probe were isolated and are currently being characterized. The region coding for the mature GtH IIb was expressed in a bacterial expression vector resulting in the production of a recombinant protein. In vitro folding resulted in a protein only 1.3% of which displaced the native common carp GtH II in a RIA. Therefore, the common carp GtH RIA was utilized for the physiological studies at the current phase of the project. Two non-functional sites were identified along the brain-pituitary gonadal axis in the immature black carp. The pituitary is refractory to GnRH stimulation due to a block proximal to the activation of PKA and PKC probably at the level of GnRH receptors. The gonads, although capable of producing steroids, are refractory to gonadotropic stimulation but do respond to cAMP antagonists, indicating a block at the GtH receptor level. Attempts to advance puberty in 2 and 3 y old black carp showed that testosterone (T) stimulates GtH synthesis in the pituitary and increases its sensitivity to GnRh. A 2 month treatment combining T+GnRH increased the circulating GFtH level in 3 y old fish. Addition of domperidone to such a treatment facilitated both the accumulation of GtH in the pituitary and its response to GnRH. The cDNA of striped bass GtH a, Ib and IIb subunits were amplified, isolated, cloned and sequenced, and their deduced a.a. sequences were compared with those of other teleosts. A ribonuclease protection assay was developed for a sensitive and simultaneous determination of all GtH subunits, and of b-actin mRNAs of the striped bass. GnRH stimulated dramatically the expression of the a and GtH IIb subunits but the level of GtH Ib mRNA increased only moderately. These findings suggest that GtH-II, considered in salmonids to be involved only in final stages of gametogenesis, can be induced by GnRH to a higher extent than GtH-I in juvenile striped bass. The native GtH II of the striped bass was isolated and purified, and an ELISA for its determination was developed. The production of all recombinant striped bass GtH subunits is in progress using the insect cell (Sf9) culture and the BAC-TO-BAC baculovirus expression system. A recombinant GtH IIb subunit has been produced already, and its similarity to the native subunit was confirmed. The yield of the recombinant glycoprotein can reach 3.5 mg/ml after 3 days culture. All male striped bass reach puberty after 3 y. However, precocious puberty was discovered in 1 and 2 y old males. Females become vitellogenic during their 4th year. In immature 2 y old females, T treatment elevates the pituitary GtH II content while GnRH only potentiates the effect. However, in males GnRH and not T affects GtH accumulation in the pituitary. Neither GnRH, nor T treatment resulted in gonadal growth in 2 y old striped bass, indicating that either the accumulated GtH II was not released, or if released, the gonads were refractory to GtH stimulation, similar to the situation in the immature black carp. In 3 y old female striped bass, 150 day GnRHa treatment resulted in an increase in GSI, while T treatment, with or without GnRHa, resulted in a decrease in oocyte diameter, similar to the effect seen in the black carp. Further attempts to advance puberty in both fish species should take into account the positive effect of T on pituitary GtH and its negative effect of ovarian growth.
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